Other Characterized Proteins Similar to serB
Finding the characterized proteins for serB (phosphoserine phosphatase) in L-serine biosynthesis
Or see clustering for step serB
Or see all steps for L-serine biosynthesis
Or cluster curated proteins matching a keyword
Definition of serB
- Curated proteins or TIGRFams with EC 3.1.3.3
- Ignore hits to items matching EC 1.1.1.95 when looking for 'other' hits
- Ignore hits to P60487 when looking for 'other' hits (pyridoxal phosphatase (EC 3.1.3.74). Chronophin; Pyridoxal 5'-phosphate phosphatase; Pyridoxal phosphate phosphatase; PLP phosphatase; EC 3.1.3.16; EC 3.1.3.74)
- Ignore hits to Q96GD0 when looking for 'other' hits (pyridoxal phosphatase (EC 3.1.3.74). Chronophin; Pyridoxal phosphate phosphatase; PLP phosphatase; EC 3.1.3.16; EC 3.1.3.74)
- Ignore hits to Q9P376 when looking for 'other' hits (CTD phosphatase Fcp1; EC 3.1.3.3. RNA polymerase II subunit A C-terminal domain phosphatase; CTD phosphatase fcp1; EC 3.1.3.16)
- Ignore hits to O07014 when looking for 'other' hits (phosphoserine phosphatase (EC 3.1.3.3))
- Ignore hits to Q3ZBF9 when looking for 'other' hits (Chronophin; Protein serine phosphatase; Pyridoxal phosphate phosphatase; PLP phosphatase; EC 3.1.3.16; EC 3.1.3.74)
- Ignore hits to P00634 when looking for 'other' hits (alkaline phosphatase (EC 3.1.3.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.108; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.108; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1))
- Ignore hits to P0AE22 when looking for 'other' hits (acid phosphatase (EC 3.1.3.2). class B acid phosphatase; EC 3.1.3.2. Class B acid phosphatase; CBAP; EC 3.1.3.2. acid phosphatase / phosphotransferase (EC 3.1.3.2; EC 3.1.3.31; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.34; EC 3.1.3.3). acid phosphatase / phosphotransferase (EC 3.1.3.2; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.34; EC 3.1.3.3))
- Ignore hits to P17906 when looking for 'other' hits (phosphoserine phosphatase (EC 3.1.3.3). Phosphoserine phosphatase RsbX; Sigma-B negative effector; EC 3.1.3.3)
- Ignore hits to P40399 when looking for 'other' hits (phosphoserine phosphatase (EC 3.1.3.3). Phosphoserine phosphatase RsbU; Sigma factor SigB regulation protein RsbU; EC 3.1.3.3)
- Ignore hits to P94526 when looking for 'other' hits (Sugar-phosphatase AraL; Arabinose operon protein AraL; Phosphoserine phosphatase; EC 3.1.3.23; EC 3.1.3.3)
- UniProt sequence Q9K8N3: SubName: Full=BH2972 protein {ECO:0000313|EMBL:BAB06691.1};
- UniProt sequence A0A1R2PVY2: RecName: Full=Phosphoserine phosphatase {ECO:0000256|ARBA:ARBA00015196}; EC=3.1.3.3 {ECO:0000256|ARBA:ARBA00012640}; AltName: Full=O-phosphoserine phosphohydrolase {ECO:0000256|ARBA:ARBA00031693};
- UniProt sequence N9V397: SubName: Full=Phosphoglycerate mutase family protein, putative {ECO:0000313|EMBL:ENY63406.1};
- Predicted: UniProt sequence A0A6B4WGC7: SubName: Full=DUF1015 domain-containing protein {ECO:0000313|EMBL:MBD5586605.1};
- Comment: CA265_RS22635 (see Fitness Browser) is diverged serB and is auxotrophic. It also appears to be fused to diverged serA, but there is another serA in the genome (CA265_RS09010), so CA265_RS22635 may not be a functional dehydrogenase. Echvi_2777 (see Fitness Browser) from Echinicola vietnamensis KMM 6221 is a diverged serB and is auxotrophic, rescued by serine. It is fused to serA and probably provides that activity as well. Hits to serA are ignored because of serAB fusions. Some curated resources link this EC number to protein phosphatases, or to non-specific periplasmic phosphatases, that are unlikely to play a role in serine biosynthesis. These are all marked ignore (P60487, Q96GD0, Q3ZBF9, P00634, P0AE22, Q9P376, O07014). Similarly, Swiss-Prot annotates RsbX (P17906) and RsbU (P40399) with this EC number, but they probably act on proteins. And, AraL (P94526) is ignored because although it does have activity on phosphoserine, it is a promiscuous phosphatase (supplementary material of PMC4413258). PMID:25848029 does show that BH2972 (Q9K8N3) and X3MFA4 (now A0A1R2PVY2) have this activity. 5zr2C (N9V397) is serB in Entamoeba histolytica (see PMID:30935984) In Clostridiodes difficile, the serine synthesis operon includes CDIF630erm_01132, a DUF1015 protein which is speculated to replace serB (PMC6110889). This subfamily is usually colocated with serA or serC3 (see ADT23_RS05570 or A0A6B4WGC7), and is structurally related to the N-terminal domain of serine kinases (using foldseek). Furthermore, genomes that encode this subfamily usually lack any known form of serB.
Fetched 35 sequences
Running ublast to find other characterized proteins with 40% identity and 70% coverage
Found hits to 2 other characterized sequences. (Found 33 hits including self hits.)
Removing ignored items reduces this to 1 close sequences.
Close sequences
(Sequences that are similar to these will not be high-confidence candidates for serB.)
C5H3W1 glycerol-3-phosphate dehydrogenase (NAD+) (EC 1.1.1.8) from Dunaliella viridis
PFams: Hydrolase, HAD, NAD_Gly3P_dh_N, NAD_Gly3P_dh_C
701 amino acids: PaperBLAST, CDD, BRENDA::C5H3W1
40% identical to
Q5M819 phosphoserine phosphatase (EC 3.1.3.3) from Rattus norvegicus
PFams: Hydrolase, HAD
225 amino acids: PaperBLAST, CDD
Close but ignored sequences
(Sequences that are similar to these will still be high-confidence candidates for serB.)
A4VGK3 phosphoglycerate dehydrogenase (EC 1.1.1.95) from Pseudomonas stutzeri
PFams: 2-Hacid_dh, 2-Hacid_dh_C
468 amino acids: PaperBLAST, CDD, BRENDA::A4VGK3
51% identical to
Echvi_2777 fused D-3-phosphoglycerate dehydrogenase / phosphoserine phosphatase (EC 1.1.1.95; EC 3.1.3.3) from Echinicola vietnamensis KMM 6221, DSM 17526
PFams: HAD, 2-Hacid_dh, 2-Hacid_dh_C, NAD_binding_2
630 amino acids: PaperBLAST, CDD