Family Search for PF01861 (BpsA_C)

April 2024: GapMind for amino acid biosynthesis has been updated to include newly-discovered enzymes and comparative genomics predictions (see details)

Running HMMer for PF01861

PF01861 hits 15 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

MJ1273 conserved hypothetical protein from Methanocaldococcus jannaschii DSM 2661
Q58669 N(4)-bis(aminopropyl)spermidine synthase from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Aligns to 105:349 / 350 (70.0%), covers 100.0% of PF01861, 416.1 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...(Theth1513, YP_004660662), Methanocaldococcus jannaschii (MJ1273, Q58669; MJ0675, Q58088), Desulfurobacterium thermolithotrophum (Dester0229, YP_004280945),...”
  • “...archaeal hyperthermophile, has two T. kodakarensis BpsA orthologs, MJ1273 and MJ0675. MJ1273 is highly homologous to T. kodakarensis BpsA and belongs to...”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...YP_004660662), Methanocaldococcus jannaschii (MJ1273, Q58669; MJ0675, Q58088), Desulfurobacterium thermolithotrophum (Dester0229, YP_004280945), Thermovirga...”

Q8U1U4 N(4)-bis(aminopropyl)spermidine synthase from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
PF1111 hypothetical protein from Pyrococcus furiosus DSM 3638
Aligns to 105:348 / 349 (69.9%), covers 100.0% of PF01861, 413.5 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...TK0147, Q5JFG9), Pyrococcus furiosus (PF1111, Q8U1U4; ACAPT [PF0127], Q8U4G1), Methanotorris igneus (Metig0730, YP_004484345), Thermotoga thermarum (Theth1513,...”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...A) includes the thermophilic T. kodakarensis BpsA orthologs (PF1111 from P. furiosus, Metig0730 from Methanotorris igneus, and TTHC0171 from T. thermophilus). A...”
  • “...Q5JIZ3; TK0147, Q5JFG9), Pyrococcus furiosus (PF1111, Q8U1U4; ACAPT [PF0127], Q8U4G1), Methanotorris igneus (Metig0730, YP_004484345), Thermotoga thermarum...”
• Experimental and computational analysis of the secretome of the hyperthermophilic archaeon Pyrococcus furiosus
  Schmid, Extremophiles : life under extreme conditions 2013
  • “...33 No PF1047 18893110 Hypothetical protein PF1047 FUN14 family (unknown function) 10 2 27 3 PF1111 18893184 Hypothetical protein PF1111 Protein of unknown function DUF43 40 5 13 No PF1500 18893630 Hypothetical protein PF1500 PRC-barrel domain 10 6 61 No PF1837 18894020 Hypothetical protein PF1837 ATP-grasp...”

2qm3A / Q8U1U4 Crystal structure of a predicted methyltransferase from pyrococcus furiosus
Aligns to 101:338 / 338 (70.4%), covers 97.5% of PF01861, 403.7 bits

• Ligand: calcium ion (2qm3A)

bpsA / Q5JIZ3 N⁴-bis(aminopropyl)spermidine synthase (EC 2.5.1.128) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (see 2 papers)
BPSA_THEKO / Q5JIZ3 N(4)-bis(aminopropyl)spermidine synthase; Branched-chain polyamine synthase A; EC 2.5.1.128 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
Q5JIZ3 N4-bis(aminopropyl)spermidine synthase (EC 2.5.1.128) from Thermococcus kodakarensis (see paper)
TK1691 hypothetical protein from Thermococcus kodakaraensis KOD1
Aligns to 106:350 / 351 (69.8%), covers 100.0% of PF01861, 401.9 bits

• function: Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine. Can also use spermine to produce N(4)-aminopropylspermine.
  catalytic activity: 2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 H(+) + N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine (RHEA:44132)
  disruption phenotype: Grows at 85 degrees Celsius with a slightly longer lag phase, but is unable to grow at 93 degrees Celsius.
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...by the MASCOT database. TK0545, TK0548, TK0967, and TK1691 were identified as candidate enzymes, and the corresponding genes were individually cloned and...”
  • “...was measured. Of the four candidates, TK1691 (BpsA) was found to synthesize N4-bis(aminopropyl)spermidine from spermidine via N4-aminopropylspermidine. Compared...”
• Immobilization of a Broad Range of Polypeptides on the Frustule of the Diatom Thalassiosira pseudonana
  Shimakawa, Applied and environmental microbiology 2022 (secret)
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...Q8ZXM4), Thermococcus kodakarensis (TK1691 [BpsA], Q5JIZ3; TK0147, Q5JFG9), Pyrococcus furiosus (PF1111, Q8U1U4; ACAPT [PF0127], Q8U4G1), Methanotorris...”

5xncB / Q5JIZ3 Crystal structure of the branched-chain polyamine synthase (bpsa) in complex with n4-aminopropylspermidine and 5-methylthioadenosine (see paper)
Aligns to 109:353 / 354 (69.2%), covers 100.0% of PF01861, 401.8 bits

• Ligands: fe (iii) ion; 5'-deoxy-5'-methylthioadenosine; n,n-bis(3-aminopropyl)butane-1,4-diamine (5xncB)

O67635 N(4)-bis(aminopropyl)spermidine synthase from Aquifex aeolicus (strain VF5)
Aligns to 110:354 / 355 (69.0%), covers 100.0% of PF01861, 387.8 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...(Tlie1345, YP_004933171), Aquifex aeolicus (aq1754, O67635; aq062, O66473), Thermoanaerobacter tengcongensis (TTE1898, Q8R8U3), Caldicellulosiruptor bescii...”

O28662 N(4)-bis(aminopropyl)spermidine synthase from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)
AF1611 conserved hypothetical protein from Archaeoglobus fulgidus DSM 4304
Aligns to 123:368 / 371 (66.3%), covers 99.6% of PF01861, 372.1 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...SpeE, Q72K55), Archaeoglobus fulgidus (AF1611, O28662), Ferroglobus placidus (Ferp1880, YP_003436295), Archaeoglobus profundus (Arcpr0370, YP_003400112),...”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...Q72L89; SpeE, Q72K55), Archaeoglobus fulgidus (AF1611, O28662), Ferroglobus placidus (Ferp1880, YP_003436295), Archaeoglobus profundus (Arcpr0370,...”
  • “...*Thermus thermophilus (TTC0171) *Archaeoglobus fulgidus (AF1611) 69 100 *Ferroglobus placidus (Ferp1880) *Archaeoglobus profundus (Arcpr0370) 72 Methanococcus...”
• DNA-binding proteins and evolution of transcription regulation in the archaea
  Aravind, Nucleic acids research 1999
  • “...No. 23 A conserved family of archaeal proteins (e.g. AF1611 shown in Fig. 2), which is also represented in thermophilic bacteria, contains a wHTH domain fused...”

TTC0171 No description from Thermus thermophilus HB27
Q72L89 N(4)-bis(aminopropyl)spermidine synthase from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
Aligns to 113:352 / 353 (68.0%), covers 98.8% of PF01861, 369.1 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...marinus (Rmar0533, YP_003289823), Thermus thermophilus (TTC0171, Q72L89; SpeE, Q72K55), Archaeoglobus fulgidus (AF1611, O28662), Ferroglobus placidus (Ferp1880,...”
  • “...marinus (Rmar0533) 100 100 *Thermus thermophilus (TTC0171) *Archaeoglobus fulgidus (AF1611) 69 100 *Ferroglobus placidus (Ferp1880) *Archaeoglobus profundus...”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...(Rmar0533, YP_003289823), Thermus thermophilus (TTC0171, Q72L89; SpeE, Q72K55), Archaeoglobus fulgidus (AF1611, O28662), Ferroglobus placidus (Ferp1880,...”

6j28A / Q72L89 Crystal structure of the branched-chain polyamine synthase c9 mutein from thermus thermophilus (tth-bpsa c9) in complex with n4- aminopropylspermidine and 5'-methylthioadenosine (see paper)
Aligns to 104:344 / 345 (69.9%), covers 98.8% of PF01861, 368.4 bits

• Ligands: n,n-bis(3-aminopropyl)butane-1,4-diamine; 5'-deoxy-5'-methylthioadenosine; fe (iii) ion (6j28A)

MJ0675 conserved hypothetical protein from Methanocaldococcus jannaschii DSM 2661
Q58088 Uncharacterized protein MJ0675 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
Aligns to 129:379 / 391 (64.2%), covers 99.6% of PF01861, 337.5 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...Methanocaldococcus jannaschii (MJ1273, Q58669; MJ0675, Q58088), Desulfurobacterium thermolithotrophum (Dester0229, YP_004280945), Thermovirga lienii (Tlie1345,...”
  • “...has two T. kodakarensis BpsA orthologs, MJ1273 and MJ0675. MJ1273 is highly homologous to T. kodakarensis BpsA and belongs to aminopropyltransferase group A....”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...jannaschii (MJ1273, Q58669; MJ0675, Q58088), Desulfurobacterium thermolithotrophum (Dester0229, YP_004280945), Thermovirga lienii (Tlie1345, YP_004933171),...”

TTE1898 predicted methyltransferases from Thermoanaerobacter tengcongensis MB4
Aligns to 110:352 / 358 (67.9%), covers 99.2% of PF01861, 304.0 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...O67635; aq062, O66473), Thermoanaerobacter tengcongensis (TTE1898, Q8R8U3), Caldicellulosiruptor bescii (Athe2198, YP_0025740 46); Rhodothermus marinus...”
  • “...*Aquifex aeolicus (aq1754) *Thermoanaerobacter tengcongensis (TTE1898) 100 99 *Caldicellulosiruptor bescii (Athe2198) *Rhodothermus marinus (Rmar0533) 100 100...”
• A cross-genomic approach for systematic mapping of phenotypic traits to genes
  Jim, Genome research 2004
  • “...TTE0818 TTE1866 TTE0714 TTE2659 TTE0820 TTE1891 TTE1892 TTE1893 TTE1898 TTE2657 TTE0705 TTE0715 TTE1551 TTE2658 TTE2633 TTE2194 MesJ4 MesJ 5.38 5.06 5.73 4.78...”
• A complete sequence of the T. tengcongensis genome
  Bao, Genome research 2002
  • “...superfamily II (TTE1889), predicted methyltransferase (TTE1898), uncharacterized Fe-S center proteins (TTE0177), uncharacterized Fe-S protein PflX (TTE1779),...”

Q6LWQ1 N(4)-bis(aminopropyl)spermidine synthase C-terminal domain-containing protein from Methanococcus maripaludis (strain S2 / LL)
MMP1657 conserved hypothetical protein from Methanococcus maripaludis S2
Aligns to 128:378 / 390 (64.4%), covers 99.2% of PF01861, 251.9 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...YP_003400112), Methanococcus maripaludis (MMP1657, Q6LWQ1), Methanococcus aeolicus (Maeo0142, YP_001324346), Rhodopseudomonas palustris (RPB2044, Q2IYF9),...”
• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...(Arcpr0370, YP_003400112), Methanococcus maripaludis (MMP1657, Q6LWQ1), Methanococcus aeolicus (Maeo0142, YP_001324346), Rhodopseudomonas palustris (RPB2044,...”
  • “...1874 jb.asm.org to group D. Methanococcus maripaludis MMP1657 is homologous to MJ0675, with both predicted to be RNA methylases. Methyltransferases transfer...”

Q2IYF9 N(4)-bis(aminopropyl)spermidine synthase C-terminal domain-containing protein from Rhodopseudomonas palustris (strain HaA2)
Aligns to 112:314 / 383 (53.0%), covers 74.9% of PF01861, 150.0 bits

• Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
  Okada, Journal of bacteriology 2014
  • “...(Maeo0142, YP_001324346), Rhodopseudomonas palustris (RPB2044, Q2IYF9), Clostridium cellulovorans (Clocel1945, YP_003843453), and Bacillus cereus (IGE05445,...”

PP5395 conserved hypothetical protein from Pseudomonas putida KT2440
Aligns to 9:264 / 281 (91.1%), covers 79.4% of PF01861, 66.4 bits

• In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants
  Fernández, Microbial biotechnology 2013
  • “...regulation. Finally, two of the transcriptional fusions found in the four types the plants tested (PP5395 and PP3698) corresponded to genes of unknown function that are exclusively found in the KT2440 strain. A set of 11 transcriptional fusions were isolated from more than one type of...”

PP_5395 bis-aminopropyl spermidine synthase family protein from Pseudomonas putida KT2440
Aligns to 7:262 / 279 (91.8%), covers 79.4% of PF01861, 66.4 bits

• Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins in Pseudomonas putida
  Huertas-Rosales, Frontiers in molecular biosciences 2021
  • “...domain PP_4793 Hypothetical protein PP_5099 Hypothetical protein PP_5209 Hypothetical protein FliL-like protein PP_5232 Hypothetical protein PP_5395 Hypothetical protein Branched-chain polyamine synthase domain FIGURE 1 Distribution of fold enrichment (left) and 10 Log 10 ( p -value) scores (right) data of overrepresented RNA sequences associated to each...”

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