Family Search for PF01969 (Ni_insertion)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF01969 hits 13 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
D9S105 Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein from Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P)
Aligns to 4:378 / 391 (95.9%), covers 99.7% of PF01969, 507.7 bits
GSU0141 conserved hypothetical protein from Geobacter sulfurreducens PCA
Aligns to 4:379 / 388 (96.9%), covers 100.0% of PF01969, 484.8 bits
slr1411 unknown protein from Synechocystis sp. PCC 6803
Aligns to 5:404 / 422 (94.8%), covers 99.7% of PF01969, 470.1 bits
- Biochemical elucidation of citrate accumulation in Synechocystis sp. PCC 6803 via kinetic analysis of aconitase
Nishii, Scientific reports 2021 - “...; fatty acid desaturase K10255 25.4 3.9 sll0622 nadA ; quinolinate synthetase K03517 25.0 4.8 slr1411 Unknown protein K09121 25.0 5.2 slr1462 Unknown protein K06883 24.6 6.5 sll8019 Hypothetical protein 24.6 7.9 sll0757 purF ; amidophosphoribosyltransferase K00764 24.3 9.6 BLAST search for ATP-citrate lyase, citryl-CoA synthetase,...”
- Phylogenomic analysis of the Chlamydomonas genome unmasks proteins potentially involved in photosynthetic function and regulation
Grossman, Photosynthesis research 2010 - “...(sll1323), atpF (sll1324), atp D (sll1325), atpA (sll1326), and atpC (sll1327). slr1413 is upstream, and slr1411 and sll0216 are downstream of the ATP synthase operon, respectively, and neither is co-expressed with atp1 . All of the genes of the ATP synthase operon are depicted as light...”
- “...except for atp1 ; this arrow is red-filled . Arrows representing genes outside the operon, slr1411, slr1413, and sll0216, are unfilled and dark gray-filled Phenotypic analysis of GreenCut mutants Identification of numerous proteins potentially involved in photosynthetic function allows for the exploitation of reverse genetic approaches...”
larC2 / F9UST1 pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel chelatase (EC 4.99.1.12) from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) (see 3 papers)
LARC_LACPL / F9UST1 Pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel insertion protein; P2TMN nickel insertion protein; Lactate racemase accessory protein LarC; Lactate racemase activation protein LarC; Lactate racemase maturation protein LarC; Lactate racemization operon protein LarC; Nickel-pincer cofactor biosynthesis protein LarC; EC 4.99.1.12 from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1) (Lactobacillus plantarum) (see 3 papers)
F9UST1 pyridinium-3,5-bisthiocarboxylic acid mononucleotide nickel chelatase (EC 4.99.1.12) from Lactiplantibacillus plantarum (see 2 papers)
Aligns to 4:406 / 420 (96.0%), covers 100.0% of PF01969, 455.6 bits
- function: Involved in the biosynthesis of a nickel-pincer cofactor ((SCS)Ni(II) pincer complex). Binds Ni(2+), and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor. Is required for the activation of the lactate racemase LarA. May also be involved in the activation of other nickel-pincer cofactor-dependent enzymes.
catalytic activity: Ni(II)-pyridinium-3,5-bisthiocarboxylate mononucleotide = Ni(2+) + pyridinium-3,5-bisthiocarboxylate mononucleotide (RHEA:54784)
disruption phenotype: Deletion of this gene leads to a loss of lactate racemase activity. - Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea.
Pfeiffer, Genes 2021 - “...F9UST0 [ 331 ] 27114550 LarB family protein 9h HVO_2381 - possibly 31% lp_0106/ lp_0107 F9UST1 [ 331 ] 27114550 LarC family protein 9h HVO_0190 - possibly 34% lp_0109 F9UST4 [ 331 ] 27114550 LarE family protein 9i HVO_1660 dacZ self [ 37 ] 30884174 9i...”
HVO_2381 conserved hypothetical protein TIGR00299 from Haloferax volcanii DS2
Aligns to 4:479 / 493 (96.6%), covers 100.0% of PF01969, 426.7 bits
EF1827 conserved hypothetical protein from Enterococcus faecalis V583
Aligns to 3:314 / 326 (95.7%), covers 80.3% of PF01969, 359.7 bits
lp_0106 LarC family nickel insertion protein from Lactiplantibacillus plantarum WCFS1
Aligns to 4:263 / 263 (98.9%), covers 62.7% of PF01969, 282.9 bits
- Transcriptional analysis of the molecular mechanism underlying the response of Lactiplantibacillus plantarum to lactic acid stress conditions
Jang, Heliyon 2023 - “...operon protein lp_0104 COG3875 larC1 1.12 0.000485 0.0031477 2.52 1.65E-22 1.09E-20 lactate racemization operon protein lp_0106 COG1641 lp_2993 1.71 1.22E-16 6.55E-15 2.38 3.08E-31 2.82E-29 nucleotide-binding protein lp_2993 COG0589 lp_2448 2.26 0.0285048 0.0826832 2.34 0.0237935 0.0773064 prophage P2a protein regulator lp_2448 COG1396 lp_3178 2.43 4.62E-34 5.39E-32 2.33...”
- “...and membrane proteins (lp_3177, lp_2949, lp_1684). Interestingly, the expression of lactate racemization-related genes (lp_0105, lp_0104, lp_0106, and lp_1017) increased only following dl -lactic acid treatment ( Table 1 ). The results of the transcriptome analysis were validated using qRT-PCR. Gene expression data were similar to those...”
LSA0786 Hypothetical protein from Lactobacillus sakei subsp. sakei 23K
Aligns to 4:274 / 274 (98.9%), covers 62.7% of PF01969, 278.8 bits
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...lsa0757 Hypothetical protein 0.8 LSA0773 lsa0773 Hypothetical protein 0.9 0.6 LSA0784 lsa0784 Hypothetical protein -2.6 LSA0786 lsa0786 Hypothetical protein -2.0 LSA0787 lsa0787 Hypothetical protein -1.7 LSA0790 lsa0790 Hypothetical protein, ATP utilizing enzyme PP-loop family -2.5 LSA0827 lsa0827 Hypothetical lipoprotein precursor 0.8 U LSA0828 lsa0828 Hypothetical protein...”
6bwqA / F9UST1 Larc2, thE C-terminal domain of a cyclometallase involved in the synthesis of the npn cofactor of lactate racemase, in complex with mnctp (see paper)
Aligns to 1:135 / 144 (93.8%), covers 35.7% of PF01969, 162.0 bits
- Ligand: cytidine-5'-triphosphate (6bwqA)
LSA0787 Hypothetical protein from Lactobacillus sakei subsp. sakei 23K
Aligns to 4:148 / 156 (92.9%), covers 36.8% of PF01969, 161.5 bits
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...lsa0773 Hypothetical protein 0.9 0.6 LSA0784 lsa0784 Hypothetical protein -2.6 LSA0786 lsa0786 Hypothetical protein -2.0 LSA0787 lsa0787 Hypothetical protein -1.7 LSA0790 lsa0790 Hypothetical protein, ATP utilizing enzyme PP-loop family -2.5 LSA0827 lsa0827 Hypothetical lipoprotein precursor 0.8 U LSA0828 lsa0828 Hypothetical protein 0.7 LSA0829 lsa0829 Hypothetical integral...”
lp_0107 nickel insertion protein from Lactiplantibacillus plantarum WCFS1
Aligns to 1:129 / 143 (90.2%), covers 34.4% of PF01969, 157.6 bits
MM_1613 conserved protein from Methanosarcina mazei Goe1
Aligns to 1:141 / 196 (71.9%), covers 31.7% of PF01969, 78.6 bits
MK0293 Uncharacterized conserved protein from Methanopyrus kandleri AV19
Aligns to 3:153 / 176 (85.8%), covers 35.2% of PF01969, 75.4 bits
- A five-residue motif for the design of domain swapping in proteins
Nandwani, Nature communications 2019 - “...was next introduced into a -hairpin loop in the C-terminal domain of the hyperthermophilic protein MK0293 (MK-Ctd), a protein domain derived from the protein MK0293 from Methanopyrus kandleri AV19 (PDB ID: 3C19, residues 98178). MK-Ctd was chosen because it is unrelated to the cystatins, folds to...”
- “...5 Domain swapping in MK-Ctd. a Structure of the C-terminal domain of the hyperthermophilic protein MK0293 (MK-Ctd) (PDB ID: 3C19) is shown. Different secondary structural elements, the N-termini and C-termini, and loop1, loop2, and loop3 of MK-Ctd are indicated. b Size exclusion profiles of MK-Ctd, L1MK-Ctd,...”
Or search for genetic data about PF01969 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory