Family Search for PF07933 (DUF1681)

April 2024: GapMind for amino acid biosynthesis has been updated to include newly-discovered enzymes and comparative genomics predictions (see details)

PF07933 hits 18 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

W8CD89 NECAP-like protein CG9132 from Ceratitis capitata
Aligns to 3:158 / 247 (63.2%), covers 99.4% of PF07933, 212.1 bits

• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...thaliana (Uniprot accession Q84WV7), Trichinella pseudospiralis (A0A0V1JQ20), Caenorhabditis elegans (Q9N489), Echinococcus multilocularis (A0A087VZS0), Ceratitis capitata (W8CD89), Homo sapiens (Q8NC96) and Mus musculus (Q9CR95) identifies conserved motifs and residues for interaction with AP2. Gl NECAP1 presents partial conservation, with a WXXF motif (orange) shifted to the N-terminus...”

Q6DBU3 LOC553536 protein (Fragment) from Danio rerio
Aligns to 7:164 / 258 (61.2%), covers 100.0% of PF07933, 211.9 bits

• Proteomic analysis of zebrafish (Danio rerio) embryos exposed to benzyl benzoate
  Kwon, Environmental science and pollution research international 2023
  • “...type V alpha-3b 0.575 1.6E-03 25 A5PLK2 Phospholysine phosphohistidine inorganic pyrophosphate phosphatase 0.382 9.3E-11 26 Q6DBU3 LOC553536 protein 0.651 3.7E-02 27 Q6PC37 Nucleoside diphosphate kinase 0.65 3.7E-02 28 Q32Q48 Rbx1 protein 0.01 4.1E-16 29 Q6P3I0 HEAT repeat-containing 3 0.624 1.4E-02 30 A4JYG8 Cntn2 0.101 4.1E-16 31...”

NECP2_RAT / Q6P756 Adaptin ear-binding coat-associated protein 2; NECAP-2 from Rattus norvegicus (Rat) (see paper)
Aligns to 6:163 / 263 (60.1%), covers 99.4% of PF07933, 207.3 bits

• function: Involved in endocytosis.
  subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).
• Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome
  Barker, The Biochemical journal 2024 (no snippet)
• Mechanism of electroacupuncture and herb-partitioned moxibustion on ulcerative colitis animal model: A study based on proteomics
  Qi, World journal of gastroenterology 2022
  • “...1.26 0.002 0.80 0.004 P31399 ATP synthase subunit d, mitochondrial Atp5h 1.34 0.003 0.77 0.004 Q6P756 Adaptin ear-binding coat-associated protein 2 Necap2 1.48 0.011 0.83 0.023 P19511 ATP synthase F (0) complex subunit B1, mitochondrial Atp5f1 1.24 0.003 0.83 0.004 A0A0G2K950 3-phosphoadenosine 5-phosphosulfate synthase 2 Papss2...”
• Inflect: Optimizing Computational Workflows for Thermal Proteome Profiling Data Analysis
  McCracken, Journal of proteome research 2021
  • “...Pip5k2c 31543514 Q9R0J8 legumain Lgmn Prsc1 40786432 Q10758 keratin, type II cytoskeletal8 Krt8 Krt28 40018562 Q6P756 adaptin ear-binding coat-associated protein2 Necap2 164519066 P14270 cAMP-specific 3,5-cyclic phosphodiesterase 4D Pde4d 32189350 Q09073 ADP/ATP translocase2 Slc25a5 Ant2 29789307 O88658 kinesin-like protein KIF1B Kif1b 21489985 Q8VHE9 all-trans-retinol 13,14-reductase Retsat Ppsig...”
• Comparative proteomic analysis of the brain and colon in three rat models of irritable bowel syndrome.
  Zhang, Proteome science 2020
  • “...CB Protein Zyx D4A7U1 metal ion binding;zinc ion binding CB Adaptin ear-binding coat-associated protein 2 Q6P756 / protein transport CB Protein Ttc1 Q66H09 unknown CB NSFL1 cofactor p47 O35987 lipid binding; ubiquitin binding CB Toll-interacting protein A2RUW1 /inflammatory response; innate immune response; signal transduction CB Cystatin-C...”
• High throughput metabolomics-proteomics investigation on metabolic phenotype changes in rats caused by Radix Scrophulariae using ultra-performance liquid chromatography with mass spectrometry.
  Lu, RSC advances 2019
  • “...Amacr Alpha-methylacyl-CoA racemase 0.6981 Down** 0.0003 L10 M0RAJ5 Prr14l Protein Prr14l 0.7129 Down** 0.0069 L11 Q6P756 Necap2 Adaptin ear-binding coat-associated protein 2 0.7407 Down* 0.0151 L12 Q80W92 Vac14 Protein VAC14 homolog 0.7429 Down* 0.0338 L13 A0A097PE04 COX2 Cytochrome c oxidase subunit 2 0.7470 Down** 0.0005 L14...”

Q3T093 Adaptin ear-binding coat-associated protein 1 from Bos taurus
Aligns to 7:164 / 275 (57.5%), covers 100.0% of PF07933, 205.5 bits

• Proteomics analysis reveals heat shock proteins involved in caprine parainfluenza virus type 3 infection
  Zhong, BMC veterinary research 2019
  • “...0.594 1.683501684 up E1BN60 Solute carrier family 30 member 1 (SLC30A1) 1 0.77 1.298701299 up Q3T093 Adaptin ear-binding coat-associated protein 1 (NECAP1) 1 0.83 1.204819277 up G3N3D6 Phosphoinositide phospholipase C(PLCH1) 1 0.823 1.215066829 up Q2YDF6 28S ribosomal protein S35, mitochondrial(MRPS35) 1 0.809 1.236093943 up Q08DH9 CCCTC-binding...”

NECP1_MOUSE / Q9CR95 Adaptin ear-binding coat-associated protein 1; NECAP endocytosis-associated protein 1; NECAP-1 from Mus musculus (Mouse) (see 4 papers)
NP_080543 adaptin ear-binding coat-associated protein 1 from Mus musculus
Aligns to 7:164 / 275 (57.5%), covers 100.0% of PF07933, 205.3 bits

• function: Involved in endocytosis.
  subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3.
• NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.
  Alazami, Journal of medical genetics 2014 (PubMed)
  • GeneRIF: NECAP1 mutation links trafficking pathway in early infantile epileptic encephalopathy.
• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...Caenorhabditis elegans (Q9N489), Echinococcus multilocularis (A0A087VZS0), Ceratitis capitata (W8CD89), Homo sapiens (Q8NC96) and Mus musculus (Q9CR95) identifies conserved motifs and residues for interaction with AP2. Gl NECAP1 presents partial conservation, with a WXXF motif (orange) shifted to the N-terminus with respect to other orthologues. (B) Ab...”
• Identification of chronic brain protein changes and protein targets of serum auto-antibodies after blast-mediated traumatic brain injury
  Harper, Heliyon 2020
  • “...kDa 0 1 L7N221 Vmn2r112 Adaptin ear-binding coat-associated protein 1 IPI00225533 30 kDa 0 1 Q9CR95 Necap1 Transcriptional activator protein Pur-beta IPI00128867 34 kDa 0 1 O35295 Purb Isoform 1 of D-aminoacyl-tRNA deacylase 1 IPI00133713 23 kDa 0 1 Q9DD18 Dtd1 Endophilin-A1 IPI00331110 (+3) 40 kDa...”
• SQYZ granules, a traditional Chinese herbal, attenuate cognitive deficits in AD transgenic mice by modulating on multiple pathogenesis processes.
  An, American journal of translational research 2018
• Sex-Specific Differences in Oxytocin Receptor Expression and Function for Parental Behavior.
  Mitre, Gender and the genome 2017
• Integrated analysis of proteome and transcriptome changes in the mucopolysaccharidosis type VII mouse hippocampus
  Parente, Molecular genetics and metabolism 2016
  • “...gene 5835; ribosomal protein L4 P61620 Sec61a1 0.004 2.58 Sec61 alpha 1 subunit (S. cerevisiae) Q9CR95 Necap1 0.016 2.57 NECAP endocytosis associated 1 P03893 Mtnd2 0.028 2.45 NADH-ubiquinone oxidoreductase chain 2 Q9ESW4 Agk 0.004 2.38 predicted gene 8546; acylglycerol kinase Q8JZR6 Slc4a8 0.015 2.35 solute carrier...”
• Sex differences in brain proteomes of neuron-specific STAT3-null mice after cerebral ischemia/reperfusion.
  Di, Journal of neurochemistry 2012
  • “...P42669 1e-015 10/39 Hypoxanthine-guanine phosphoribosyltransferase 1 (HPRT1) P00493 5e-012 3/5 Adaptin ear-binding coat-associated protein (NECAP1) Q9CR95 1e-030 4/8 Mu-crystallin homolog (CRYM) O54983 4e-015 7/13 Malate dehydrogenase, cytoplasmic (MDH1) P14152 1e-030 4/7 Fructose-bisphosphate aldolase C (ALDOC) P05063 6e-015 15/47 Dihydropteridine reductase (QDPR) Q8BVI4 6e-015 4/8 Cytochrome b-c1...”

NP_060560 adaptin ear-binding coat-associated protein 2 isoform 1 from Homo sapiens
Q9NVZ3 Adaptin ear-binding coat-associated protein 2 from Homo sapiens
Aligns to 6:163 / 263 (60.1%), covers 99.4% of PF07933, 205.3 bits

• NECAP2 controls clathrin coat recruitment to early endosomes for fast endocytic recycling.
  Chamberland, Journal of cell science 2016 (PubMed)
  • GeneRIF: NECAP2 does not regulate the clathrin-mediated endocytosis of these cargos, the degradation of EGFR or the recycling of transferrin along the slow, Rab11-dependent recycling pathway. We show that protein knockdown of NECAP2 leads to enlarged early endosomes and causes the loss of the clathrin adapter AP-1 from the organelle.
• Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif.
  Ritter, EMBO reports 2003
  • GeneRIF: Describes identification and characterization of rat NECAP1 and NECAP2 and their roles in endocytosis.
• Sertoli cell-enriched proteins in mouse and human testicular interstitial fluid
  O'Donnell, PloS one 2023
  • “...member 8 Yes P49006 MARCKSL1 * MARCKS-related protein Yes Q96N66 MBOAT7 Lysophospholipid acyltransferase 7 Yes Q9NVZ3 NECAP2 Adaptin ear-binding coat-associated protein 2 Yes P17858 PFKL ATP-dependent 6-phosphofructokinase, liver type Yes Q9UKY0 PRND Prion-like protein doppel No J3KR55 PTPN7 Tyrosine-protein phosphatase non-receptor type 7 Yes Q96F10 SAT2*...”
• Identification of Human Host Substrates of the SARS-CoV-2 Mpro and PLpro Using Subtiligase N-Terminomics.
  Luo, ACS infectious diseases 2023
• Blood Proteome Profiling Reveals Biomarkers and Pathway Alterations in Fragile X PM at Risk for Developing FXTAS.
  Zafarullah, International journal of molecular sciences 2023
  • “...Collagen alpha-1(VI) chain 116 Q08722 CD47 1.21 15.13 0.001077 0.020052 Leukocyte surface antigen CD47 117 Q9NVZ3 NECAP2 0.6 13.39 0.001083 0.020052 Adaptin ear-binding coat-associated protein 2 118 Q9BRA2 TXNDC17 0.65 13.95 0.001121 0.020576 Thioredoxin domain-containing protein 17 119 Q16401 PSMD5 0.55 12.83 0.001208 0.021994 26S proteasome...”
• Epstein-Barr Virus BGLF2 commandeers RISC to interfere with cellular miRNA function.
  Campbell, PLoS pathogens 2022
  • “...1 0.09217 Q9UKV8 EIF2C2 (Ago2) 0 35|42 5.5 0.04326 SG and PB [ 47 ] Q9NVZ3 NECAP2 0 35|33 1 0.12928 Q8NDV7 TNRC6A (GW182) 0 24|49 1.6 0.01860 SG and PB [ 47 ] Q86UK7 ZNF598 0 24|20 2.6 0.02434 SG and PB [ 47 ]...”
• Proteome of human stem cells from periodontal ligament and dental pulp.
  Eleuterio, PloS one 2013
  • “...8.02_37221 SEP11 Septin-11 Q9NVA2 102 36 12 6.32_50604 6.36_49562 NECP2 Adaptin ear-binding coat-associated protein 2 Q9NVZ3 77 39 7 8.28_31595 8.49_28435 DPSCs and PDLSCs proteome in narrow pH ranges (47, 69) To enrich knowledge of oral stem cell proteome we carried out an analysis in narrow...”

NECP2_MOUSE / Q9D1J1 Adaptin ear-binding coat-associated protein 2; NECAP endocytosis-associated protein 2; NECAP-2 from Mus musculus (Mouse) (see 2 papers)
Aligns to 6:163 / 266 (59.4%), covers 99.4% of PF07933, 205.2 bits

• function: Involved in endocytosis.
  subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3.

XP_038295023 adaptin ear-binding coat-associated protein 1 from Canis lupus familiaris
Aligns to 7:164 / 275 (57.5%), covers 100.0% of PF07933, 205.2 bits

• Whole Genome Sequencing of Giant Schnauzer Dogs with Progressive Retinal Atrophy Establishes NECAP1 as a Novel Candidate Gene for Retinal Degeneration.
  Hitti, Genes 2019
  • GeneRIF: This study, in parallel with the known retinal expression and role of NECAP1 in clathrin mediated endocytosis (CME) in synapses, presents NECAP1 as a novel candidate gene for retinal degeneration in dogs and other species.

NECP1_HUMAN / Q8NC96 Adaptin ear-binding coat-associated protein 1; NECAP endocytosis-associated protein 1; NECAP-1 from Homo sapiens (Human) (see paper)
NP_056324 adaptin ear-binding coat-associated protein 1 from Homo sapiens
Aligns to 7:164 / 275 (57.5%), covers 99.4% of PF07933, 205.0 bits

• function: Involved in endocytosis.
  subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity).
• Effects of Fluid Shear Stress on Human Intervertebral Disc Nucleus Pulposus Cells Based on Label-Free Quantitative Proteomics.
  Xie, Disease markers 2022
  • “...0.015 Down 2 O60507 TPST1 0.49 0.020 Down 3 P09972 ALDOC 0.48 0.022 Down 4 Q8NC96 NECAP1 0.48 0.002 Down 5 O00213 APBB1 0.48 0.023 Down 6 P06737 PYGL 0.47 0.022 Down 7 P98082 DAB2 0.47 0.003 Down 8 P49247 RPIA 0.47 0.007 Down 9 Q9NWM8...”
• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...Q84WV7), Trichinella pseudospiralis (A0A0V1JQ20), Caenorhabditis elegans (Q9N489), Echinococcus multilocularis (A0A087VZS0), Ceratitis capitata (W8CD89), Homo sapiens (Q8NC96) and Mus musculus (Q9CR95) identifies conserved motifs and residues for interaction with AP2. Gl NECAP1 presents partial conservation, with a WXXF motif (orange) shifted to the N-terminus with respect to...”
• Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation.
  Wrobel, Developmental cell 2019
  • GeneRIF: NECAP recruits drivers of late stages of clathrin-coated pit (CCP) formation, including SNX9, via a site distinct from where NECAP binds AP2.
• NECAP1 loss of function leads to a severe infantile epileptic encephalopathy.
  Alazami, Journal of medical genetics 2014 (PubMed)
  • GeneRIF: NECAP1 mutation links trafficking pathway in early infantile epileptic encephalopathy.
• New genetic associations detected in a host response study to hepatitis B vaccine.
  Davila, Genes and immunity 2010 (PubMed)
  • GeneRIF: Observational study of gene-disease association. (HuGE Navigator)

NECP1_RAT / P69682 Adaptin ear-binding coat-associated protein 1; NECAP endocytosis-associated protein 1; NECAP-1 from Rattus norvegicus (Rat) (see 2 papers)
NP_001025090 adaptin ear-binding coat-associated protein 1 from Rattus norvegicus
Aligns to 7:164 / 277 (57.0%), covers 100.0% of PF07933, 204.4 bits

• function: Involved in endocytosis.
  subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with the GAE domain proteins GGA1, GGA2 and GGA3 (By similarity). Interacts with AP2A2.
• Proteomic analysis of spinal cord tissue in a rat model of cancer-induced bone pain.
  Yang, Frontiers in molecular neuroscience 2022
  • “...P63041 Cplx1 12.8 3.7 Up P84087 Cplx2 12.8 3.7 Up Q1AAU6 Asap1 10.2 3.3 Up P69682 Necap1 9.7 3.3 Up P21139 Man2c1 9.6 3.3 Up Q6P7A2 Ube4a 9.4 3.2 Up P21707 Syt1 9.3 3.2 Up O35314 Chgb 8.7 3.1 Up Q4V7C6 Gmps 8.0 3.0 Up Q4AE70...”
• Space Radiation-Induced Alterations in the Hippocampal Ubiquitin-Proteome System
  Tidmore, International journal of molecular sciences 2021
  • “...Q8VHW5 O35179 P29101 P62914 Q5U216 Q9JHL4 O35314 P31647 P63045 Q5XIE8 Q9JJ31 * O35346 * P37285 P69682 Q5XIF3 Q9JKE3 P02650 P40307 P70619 Q5XIG8 Q9JMJ4 P04631 P47863 P84092 Q5XIT1 Q9WV63 P04785 P48768 Q08602 Q62829 * Q9Z0W7 P07153 * P51635 Q32ZG1 Q63635 Q9Z142 P09895 P55051 Q3B8Q0 Q64591 Q9Z1N1 P0C5X8...”
• Proteomic Analysis of Liver Proteins in a Rat Model of Chronic Restraint Stress-Induced Depression.
  Li, BioMed research international 2017
  • “...1 0.6 Vacuolar protein sorting-associated protein 33B Q63616 1 0.6 Adaptin ear-binding coat-associated protein 1 P69682 1 0.6 Cyclin-dependent kinase 9 Q641Z4 1 0.6 Zinc fingers and homeobox protein 1 Q8R515 1 0.6 Pyridoxal phosphate phosphatase Q8VD52 1 0.6 N-alpha-acetyltransferase 11 Q4V8K3 1 0.6 Protein jagged-1...”
• Characterization of the localization and function of NECAP 1 in neurons.
  Murshid, Journal of neurochemistry 2006 (PubMed)
  • GeneRIF: NECAP 1 plays a role in clathrin-mediated neuronal endocytosis, including a role in presynaptic endocytosis. (NECAP 1)

AT1G03900 ATNAP4 (Arabidopsis thaliana non-intrinsic ABC protein 4); ATPase, coupled to transmembrane movement of substances / transporter from Arabidopsis thaliana
Aligns to 12:175 / 272 (60.3%), covers 100.0% of PF07933, 199.1 bits

• Identification of Differentially-Expressed Genes in Response to Mycosphaerella fijiensis in the Resistant Musa Accession 'Calcutta-4' Using Suppression Subtractive Hybridization
  Sánchez, PloS one 2016
  • “...% Identity Locus Description/function E-value % Identity Locus Description/function 1E3 e-156 99% GSMUA_Achr5P03850_001 Uncharacterized protein At1g03900 e-155 98% ITC1587_Bchr5_P11991 serine threonine-protein kinase cbk1-like 1C2 e-135 98% GSMUA_Achr9P23930_001 UTPglucose-1-phosphate uridylyltransferase e-108 95% ITC1587_Bchr9_P27698 udp-glucose pyrophosphorylase 1D8 e-130 98% GSMUA_Achr5P03660_001 Phosphoribulokinase/Uridine kinase family protein, expressed e-130 98% ITC1587_Bchr5_P11976...”
• Dynamic Phosphoproteome Analysis of Seedling Leaves in Brachypodium distachyon L. Reveals Central Phosphorylated Proteins Involved in the Drought Stress Response
  Yuan, Scientific reports 2016
  • “...S _RAVEES(ph)DEEPEEK_ 1893 gi|357135111 uncharacterized protein LOC100843157 isoform X1 S _FTQQSTS(ph)DDDDDKAAAEPPK_ 1927 gi|357135514 uncharacterized protein At1g03900 S _LPPPPPS(ph)PVSPTDSGVPFSPFK_ 5833 gi|357136108 uncharacterized protein LOC100838789 T _GST(ph)PTSAPAHVHPK_ 2004 gi|357136280 GTP-binding protein At2g22870 S _TVDHS(ph)DAEGSR_ 2008 gi|357136419 serine/arginine repetitive matrix protein 2-like S _GFFFHPS(ph)PR_ 2056 gi|357136945 mechanosensitive ion channel...”
• Whole-transcriptome survey of the putative ATP-binding cassette (ABC) transporter family genes in the latex-producing laticifers of Hevea brasiliensis
  Zhiyi, PloS one 2015
  • “...AtABCI15 73 NAP; NO HbABCI17 KM035321 283 NBD At1g67940 AtABCI17 73 HbABCI18 KM035322 280 NBD At1g03900 AtABCI18 76 NAP; NO(ADT) HbABCI19 KM035323 300 NBD At1g03905 AtABCI19 80 HbABCI20 KM035324 329 NBD At5g02270 AtABCI20 87 *ABCA: Similar to human abca1 protein; NBD: Nucleotide binding domain (ATP binding...”
• The automatic detection of homologous regions (ADHoRe) and its application to microcolinearity between Arabidopsis and rice
  Vandepoele, Genome research 2002
  • “...At5g59480 At5g06340 At5g43420 At5g43420 At4g08100 At2g43230 At1g03900 At3g11630 At1g03900 At3g11630 At5g57140 At3g52470 At4g22730 At1g78080 0.988 0.880 0.964...”

Bm1_42615 Uncharacterized protein from Brugia malayi
Aligns to 5:160 / 213 (73.2%), covers 100.0% of PF07933, 188.1 bits

• Multiplex proteomics analysis of gender-associated proteins in Brugia malayi
  Jiang, International journal for parasitology 2012
  • “...and dynamics Bm1_57630 retinoblastoma-binding protein, putative PF00400 Uncharacterized Bm1_38615 hypothetical protein Bm1_51000 conserved hypothetical protein Bm1_42615 MGC64450 protein, putative PF07933 Bm1_41425 BM20, putative PF05823 Nematode fatty acid retinoid binding protein Bm1_21695 hypothetical protein Bm1_26585 Hypothetical UPF0205 protein C40H1.5 in chromosome III precursor, putative PF01060 Defense mechanisms...”
  • “...2 1 1 Bm1_25705 1 2 1 1 1 Bm1_04840 2 2 2 2 1 Bm1_42615 2 2 2 2 1 1 Bm1_08205 1 2 2 2 1 2 Bm1_03430 2 2 2 2 1 1 2 Bm1_51495 2 3 1 3 3 2 2 2...”

Q9N489 NECAP PHear domain-containing protein from Caenorhabditis elegans
NP_494398 NECAP PHear domain-containing protein from Caenorhabditis elegans
Aligns to 4:158 / 236 (65.7%), covers 100.0% of PF07933, 187.8 bits

• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...NECAP1 and NECAP1 orthologues from Arabidopsis thaliana (Uniprot accession Q84WV7), Trichinella pseudospiralis (A0A0V1JQ20), Caenorhabditis elegans (Q9N489), Echinococcus multilocularis (A0A087VZS0), Ceratitis capitata (W8CD89), Homo sapiens (Q8NC96) and Mus musculus (Q9CR95) identifies conserved motifs and residues for interaction with AP2. Gl NECAP1 presents partial conservation, with a WXXF...”
• A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex.
  Partlow, eLife 2019
  • GeneRIF: The site of AP2 phosphorylation is directly coordinated by residues of the NECAP PHear domain that are predicted from genetic screens in Caenorhabditis elegans.
• NECAPs are negative regulators of the AP2 clathrin adaptor complex.
  Beacham, eLife 2018
  • GeneRIF: Consistent with this model, NECAPs preferentially bind open and phosphorylated forms of AP2 in vitro and localize with constitutively open AP2 mutants in vivo. NECAPs do not associate with phosphorylation-defective AP2 mutants, implying that phosphorylation precedes NECAP recruitment.

C0NNW9 NECAP PHear domain-containing protein from Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
Aligns to 18:250 / 354 (65.8%), covers 100.0% of PF07933, 182.5 bits

• Antibody Binding Alters the Characteristics and Contents of Extracellular Vesicles Released by Histoplasma capsulatum.
  Matos, mSphere 2016

6rh6A / Q8NC96 Solution structure and 1h, 13c and 15n chemical shift assignments for the complex of necap1 phear domain with phosphorylated ap2 mu2 148- 163 (see paper)
Aligns to 12:138 / 138 (92.0%), covers 79.1% of PF07933, 182.1 bits

• Ligand: peptide (6rh6A)

AT3G58600 hypothetical protein from Arabidopsis thaliana
Q84WV7 Adaptin ear-binding coat-associated protein 1 NECAP-1 from Arabidopsis thaliana
Aligns to 32:192 / 302 (53.3%), covers 100.0% of PF07933, 178.2 bits

• Adaptor protein complex interaction map in Arabidopsis identifies P34 as a common stability regulator
  Wang, Nature plants 2023
  • “...that can be linked to endocytosis, as witnessed by the co-purification of an Arabidopsis homolog (AT3G58600) of the human ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN-1 (NECAP-1) 40 with three AP-1 subunits in TAP-MS. The interaction between NECAP-1 and AP-1 was confirmed in reciprocal TAP-MS experiments with NECAP-1 as...”
• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...Multiple sequence alignment analysis of Gl NECAP1 and NECAP1 orthologues from Arabidopsis thaliana (Uniprot accession Q84WV7), Trichinella pseudospiralis (A0A0V1JQ20), Caenorhabditis elegans (Q9N489), Echinococcus multilocularis (A0A087VZS0), Ceratitis capitata (W8CD89), Homo sapiens (Q8NC96) and Mus musculus (Q9CR95) identifies conserved motifs and residues for interaction with AP2. Gl NECAP1...”

B5XFU5 Adaptin ear-binding coat-associated protein 1 from Salmo salar
Aligns to 7:152 / 164 (89.0%), covers 79.7% of PF07933, 171.0 bits

• Global Proteomic Profiling of Piscirickettsia salmonis and Salmon Macrophage-Like Cells during Intracellular Infection
  Ortiz-Severín, Microorganisms 2020
  • “...V V C0HAE8 Phosphatidylinositol-binding clathrin assembly protein V V B9ENL7 Ras-related protein Rab-5C V V B5XFU5 Adaptin ear-binding coat-associated protein 1 P P B5X152 AP-3 complex subunit mu-1 P P B5RI38 Cathepsin l-like P P [ 12 ] C0HA36 Endophilin-B2 P P C0H980 Lysosome-associated membrane glycoprotein...”

GL50803_17195 hypothetical protein from Giardia intestinalis
A8BII4 NECAP PHear domain-containing protein from Giardia intestinalis (strain ATCC 50803 / WB clone C6)
Aligns to 3:157 / 178 (87.1%), covers 100.0% of PF07933, 142.3 bits

• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...Gl epsin Pfam: ENTH (4.8e-33) Ventral disc/PVs Ebneter 2014, Touz 2015 GL50803_3256 100/6e-37 PH A8BII4 GL50803_17195 36/61.3 1tqz 100/4.4e-52 36/61.3 NECAP-1 1tqz Hypothetical protein Gl NECAP1 Pfam: DUF1681 (5.5e-43) PVs/Cytosolic Zumthor 2016 (1tqz/Q9CR95) (100/3.6e-55) FYVE A8BDZ8 GL50803_16653 (98.17/2.1e-9) 39/92.1 3mpx 92.07/0.11 19/33.4 FYVE/RhoGEF and PH-domain containing...”
  • “...and GL50803_14855 all code for predicted C2-containing phosphoinositide kinases [ 30 , 31 ]. Protein GL50803_17195 ( Gl NECAP1) is a predicted NECAP1 homolog containing a PH-like domain. Similarly, a conserved PH-like domain found at the C-terminus of FERM proteins was correlated with high confidence to...”
• Phosphoinositide-binding proteins mark, shape and functionally modulate highly-diverged endocytic compartments in the parasitic protist Giardia lamblia
  Cernikova, PLoS pathogens 2020
  • “...2 Gl epsin Pfam: ENTH (4.8e-33) Ventral disc/PVs Ebneter 2014, Touz 2015 GL50803_3256 100/6e-37 PH A8BII4 GL50803_17195 36/61.3 1tqz 100/4.4e-52 36/61.3 NECAP-1 1tqz Hypothetical protein Gl NECAP1 Pfam: DUF1681 (5.5e-43) PVs/Cytosolic Zumthor 2016 (1tqz/Q9CR95) (100/3.6e-55) FYVE A8BDZ8 GL50803_16653 (98.17/2.1e-9) 39/92.1 3mpx 92.07/0.11 19/33.4 FYVE/RhoGEF and PH-domain...”

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