Family Search for PF09949 (APP1_cat)

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PF09949 hits 10 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

APP1_YEAST / P53933 Phosphatidate phosphatase APP1; PAP; Actin patch protein 1; EC 3.1.3.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 7 papers)
NP_014305 phosphatidate phosphatase APP1 from Saccharomyces cerevisiae S288C
YNL094W App1p from Saccharomyces cerevisiae
Aligns to 276:426 / 587 (25.7%), covers 100.0% of PF09949, 174.5 bits

• function: Mg(2+)-dependent phosphatidate (PA) phosphatase which catalyzes the dephosphorylation of PA to yield diacylglycerol. May play a role in vesicular trafficking through its PAP activity at cortical actin patches (PubMed:23071111, PubMed:23335564). Can also utilize diacylglycerol pyrophosphate and lyso-PA as substrates with specificity constants 4- and 7-fold lower, respectively, when compared with PA (PubMed:23335564).
  catalytic activity: a 1,2-diacyl-sn-glycero-3-phosphate + H2O = a 1,2-diacyl-sn- glycerol + phosphate (RHEA:27429)
  catalytic activity: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1,2- di-(9Z-octadecenoyl)-sn-glycerol + phosphate (RHEA:43244)
  cofactor: Mg(2+)
  subunit: Monomer (PubMed:23335564). Interacts with ABP1 (PubMed:14737190).
• Protein interaction networks by proteome peptide scanning
  Landgraf, PLoS biology 2004
  • “...affinity in the 1100 M range. Furthermore, fragments of Prk1 (P40494), Yir003w (P40563), and Ynl094w (P53933) were reported to bind to the Abp1 SH3 domain in vitro ( Fazi et al. 2002 ). Surprisingly, we could not identify any tetradecapeptide in the Ynl094w protein with affinity...”
• Characterization of the yeast actin patch protein App1p phosphatidate phosphatase.
  Chae, The Journal of biological chemistry 2013
  • GeneRIF: App1p also utilized diacylglycerol pyrophosphate and lyso-PA as substrates with specificity constants 4- and 7-fold lower, respectively, when compared with PA.
• The Saccharomyces cerevisiae actin patch protein App1p is a phosphatidate phosphatase enzyme.
  Chae, The Journal of biological chemistry 2012
  • GeneRIF: App1p is a phosphatidate phosphatase enzyme.
• RNA-binding proteins that inhibit RNA virus infection.
  Zhu, Proceedings of the National Academy of Sciences of the United States of America 2007
  • GeneRIF: Pus4 and App1 dramatically inhibit Brome Mosaic Virus systemic spread in plants, reduce BMV genomic plus-strand RNA accumulation, and prevent the encapsidation of a BMV RNA.
• Actin and endocytosis in budding yeast
  Goode, Genetics 2015
  • “...YDR129c RVS161 RVS161 END6 , FUS7 , SPE161 , YCR009c RVS167 RVS167 YDR388w APP1 APP1 YNL094w SRV2 SRV2 CAP , YNL138w DRS2 DRS2 FUN38 , SWA3 , YAL026c YPK1 YPK1 SLI2 , YKL126w ARV1 ARV1 YLR242c Table 2 Yeast endocytosis proteins, partners, domains and mammalian homologs...”
• SlopMap: a software application tool for quick and flexible identification of similar sequences using exact k-mer matching
  Zhbannikov, Journal of data mining in genomics & proteomics 2013
  • “...ycgB, dhaR, alkA, yfgF, yphE, mscS, parC ( E-coli , GenBank accession number: U00096.2); YNL095C, YNL094W, YNL093W, YNL092W, YNL091W, YNL090W, YNL089C, YNL088W, YNL087W, YNL085W ( Yeast , GenBank accession number: AF458977.1). We estimate the number of reads found by SlopMap with various thresholds and kmers in...”
• SLA2 mutations cause SWE1-mediated cell cycle phenotypes in Candida albicans and Saccharomyces cerevisiae
  Gale, Microbiology (Reading, England) 2009
  • “...Yang et al. , 1999 ) and all interact by the yeast 2-hybrid method with Ynl094w ( Drees et al. , 2001 ), a protein of unknown function that localizes to cortical actin patches ( Drees et al. , 2001 ) and interacts with Swe1 (...”
  • “...tempting to speculate that the morphogenesis checkpoint monitors defects in endocytosis through its interaction with Ynl094w. Physiological stresses on the yeast cell were thought to induce the morphogenesis checkpoint by perturbation of the actin cytoskeleton. However, exposure of yeast cells to osmotic stress leads to a...”
• Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals new roles for protein glycosylation in eukaryotes
  Kung, Molecular systems biology 2009
  • “...Membrane fraction YGL258W VEL1 Other YIR039C YPS6 Cell wall YDR088C SLU7 Nucleus YNL054W VAC7 Membrane/cytoplasm/vacuole YNL094W APP1 Cell cortex/cytoplasm/cytoskeleton YNL308C KRI1 Nucleolus YOR027W STI1 Cytoplasm YPL004C LSP1 Cytoplasm/cell cortex Table 2 Validated mitochondrial proteins Systematic name Common name Process Function Compartment YMR306W FKS3 Biological_process unknown 1,3--Glucan...”
• The machinery for cell polarity, cell morphogenesis, and the cytoskeleton in the Basidiomycete fungus Ustilago maydis-a survey of the genome sequence
  Banuett, Fungal genetics and biology : FG & B 2008
  • “...e86 Actin-interacting protein; actin patch component 114 APP1 YNL094W 04613 9.7 e22 Actin filament organization 115 ARC15 YIL062C 02388 2.6 e25 Subunit of...”
• Identification of putative regulatory upstream ORFs in the yeast genome using heuristics and evolutionary conservation
  Cvijović, BMC bioinformatics 2007
  • “...uORF1(6) -71 109 YMR145C NDE1 1006 uORF1(4) -165 215 YNL053W MSG5 358 uORF1(4) -104 120 YNL094W APP1 771 uORF1(3) -155 278 YNR016C ACC1 1539 uORF1(3) -342 540 YOL100W PKH2 1317 uORF2(9) uORF1(7) -338 -81 255 YOL130W ALR1 1042 uORF1(5) -103 290 YOR061W CKA2 371 uORF1(5) -103...”
• A genetic dissection of Aip1p's interactions leads to a model for Aip1p-cofilin cooperative activities
  Clark, Molecular biology of the cell 2006
  • “...YIL034c YIL138c YHR114w YIR003w YHR135c YIL095w YKL002w YKL007w YNL094w YNL084c YBR172c ENT2 SLA1 EDE1 EDS1 LSB5 ENT1 RVS161 RGD1 AKR1 BRE4 SAC6 SWA2 PKH1...”
• Protein interaction networks by proteome peptide scanning
  Landgraf, PLoS biology 2004
  • “...an affinity in the 1100 M range. Furthermore, fragments of Prk1 (P40494), Yir003w (P40563), and Ynl094w (P53933) were reported to bind to the Abp1 SH3 domain in vitro ( Fazi et al. 2002 ). Surprisingly, we could not identify any tetradecapeptide in the Ynl094w protein with...”
  • “...tandem affinity purification (TAP) technology ( Rigaut et al. 1999 ) to tag the Prk1, Ynl094w, Scp1, and Yir003w proteins, and we initially asked, by pulldown assays, whether the putative peptide targets of the Abp1 SH3 domain were available for interaction in the protein natural context....”
• More

IHV18_09890 phosphatase domain-containing protein from Bifidobacterium breve
Aligns to 217:368 / 418 (36.4%), covers 100.0% of PF09949, 163.5 bits

• Bifidobacterium breve PRL2020: Antibiotic-Resistant Profile and Genomic Detection of Antibiotic Resistance Determinants
  Di, Microorganisms 2023
  • “...6238 7495 1 NAD(+) diphosphatase IHV18_09885 gcvH 7510 7912 1 glycine cleavage system protein GcvH IHV18_09890 7958 9215 1 DUF2183 domain-containing protein IHV18_09895 9237 11,994 1 S9 family peptidase IHV18_09900 12,060 13,092 1 3-isopropylmalate dehydrogenase IHV18_09905 13,142 14,276 1 lipoateprotein ligase family protein IHV18_09910 14,428 15,148...”

Bbr_0482 phosphatase domain-containing protein from Bifidobacterium breve UCC2003
Aligns to 217:368 / 418 (36.4%), covers 100.0% of PF09949, 161.7 bits

• Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003
  Ruiz, PloS one 2013
  • “...with DUF domain H 502520 gccgacgtc Bbr_0375 Conserv. Hypothetical membrane spanning protein I 633832 cccgatgac Bbr_0482 Conserv. Hypothetical protein J 1771618 gatgaggtt - 223 nt upstream Bbr_1420 K 634533 gttcacgcg Bbr_0482 Conserv. Hypothetical protein UCC1 1167747 gataagaag Bbr_0915 Conserv. Hypothetical protein with DUF558 domain UCC3 325241...”

EX895_002183 hypothetical protein from Sporisorium graminicola
Aligns to 402:555 / 707 (21.8%), covers 99.4% of PF09949, 145.7 bits

• Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes
  Martínez-Soto, Microorganisms 2020
  • “...patch component UMAG_05949 UHOR_08467 UBRO_08467 sr16579 EX895_005119 BCV70DRAFT_213703 Actin filament organization UMAG_04613 UHOR_06638 UBRO_06638 sr15500 EX895_002183 BCV70DRAFT_202237 LIS1 Nuclear migration UMAG_03164 UHOR_04938 UBRO_04938 sr14206 EX895_005820 BCV70DRAFT_200264 Actin regulating Ser/Thr kinase UMAG_03081 UHOR_04824 UBRO_04824 sr14140 EX895_002483 - Formin; actin nucleation UMAG_12254 UHOR_06202 UBRO_06202 sr15020 EX895_001591 BCV70DRAFT_205747 Formin;...”

UMAG_04613 uncharacterized protein from Ustilago maydis 521
Aligns to 402:555 / 708 (21.8%), covers 100.0% of PF09949, 145.5 bits

• Molecular Mechanisms Involved in the Multicellular Growth of Ustilaginomycetes
  Martínez-Soto, Microorganisms 2020
  • “...BCV70DRAFT_201455 Actin-interactin gprotein; actin patch component UMAG_05949 UHOR_08467 UBRO_08467 sr16579 EX895_005119 BCV70DRAFT_213703 Actin filament organization UMAG_04613 UHOR_06638 UBRO_06638 sr15500 EX895_002183 BCV70DRAFT_202237 LIS1 Nuclear migration UMAG_03164 UHOR_04938 UBRO_04938 sr14206 EX895_005820 BCV70DRAFT_200264 Actin regulating Ser/Thr kinase UMAG_03081 UHOR_04824 UBRO_04824 sr14140 EX895_002483 - Formin; actin nucleation UMAG_12254 UHOR_06202 UBRO_06202...”

YNK4_SCHPO / Q9HGP1 Uncharacterized protein C29B5.04c from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
Aligns to 291:441 / 605 (25.0%), covers 100.0% of PF09949, 138.7 bits

SS1G_13385 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
Aligns to 282:427 / 519 (28.1%), covers 99.4% of PF09949, 126.6 bits

• An Interspecies Comparative Analysis of the Predicted Secretomes of the Necrotrophic Plant Pathogens Sclerotinia sclerotiorum and Botrytis cinerea
  Heard, PloS one 2015
  • “...to endoglucanase II C027 SS1G_09363 - SS1G_09365 glucan 1,3-beta-glucosidase precursor SS1G_09366 periplasmic beta-glucosidase precursor C028 SS1G_13385 actin patch protein 1 SS1G_13386 cutinase C029 SS1G_10165 carbohydrate esterase family 8 protein SS1G_10167 polygalacturonase 1 C030 SS1G_03610 CHP SS1G_03611 CFEM domain protein C031 SS1G_11700 chitinase 1 precursor SS1G_11703 GPI...”

TTHERM_00215970 nuclear elongation and deformation protein from Tetrahymena thermophila SB210
Aligns to 141:288 / 335 (44.2%), covers 78.1% of PF09949, 32.2 bits

• An evolutionarily conserved phosphatidate phosphatase maintains lipid droplet number and endoplasmic reticulum morphology but not nuclear morphology
  Pillai, Biology open 2017
  • “...LIPIN ) in the Tetrahymena Genome Database and designated them as TtPAH1 (TTHERM_00189270) and TtPAH2 (TTHERM_00215970). TtPah1 contains 872 amino acids and is comparable to Pah1 proteins in other organisms, whereas TtPah2 (335 amino acids) is smaller than other known lipins. Both TtPah1 and TtPah2 proteins...”
  • “...( Han and Carman, 2010 ). Sequence analysis Sequences of Tetrahymena PAH homologs (TTHERM_00189270 and TTHERM_00215970) were retrieved from the Tetrahymena Genome Database and domains were predicted with Interpro protein sequence analysis and classification tool (EMBL-EBI). Multiple sequence alignment was performed with PRALINE. Percent identity matrix...”

TTHERM_00189270 LNS2 (lipin/Ned1/SMP2) protein from Tetrahymena thermophila SB210
Aligns to 661:808 / 872 (17.0%), covers 89.7% of PF09949, 30.0 bits

• An evolutionarily conserved phosphatidate phosphatase maintains lipid droplet number and endoplasmic reticulum morphology but not nuclear morphology
  Pillai, Biology open 2017
  • “...( PAH / LIPIN ) in the Tetrahymena Genome Database and designated them as TtPAH1 (TTHERM_00189270) and TtPAH2 (TTHERM_00215970). TtPah1 contains 872 amino acids and is comparable to Pah1 proteins in other organisms, whereas TtPah2 (335 amino acids) is smaller than other known lipins. Both TtPah1...”
  • “...standard curve ( Han and Carman, 2010 ). Sequence analysis Sequences of Tetrahymena PAH homologs (TTHERM_00189270 and TTHERM_00215970) were retrieved from the Tetrahymena Genome Database and domains were predicted with Interpro protein sequence analysis and classification tool (EMBL-EBI). Multiple sequence alignment was performed with PRALINE. Percent...”

Q9XXT5 phosphatidate phosphatase (EC 3.1.3.4) from Caenorhabditis elegans (see paper)
NP_506380 LNS2/PITP domain-containing protein from Caenorhabditis elegans
Aligns to 554:692 / 794 (17.5%), covers 76.8% of PF09949, 28.6 bits

• Lipin is required for efficient breakdown of the nuclear envelope in Caenorhabditis elegans.
  Gorjánácz, Journal of cell science 2009 (PubMed)
  • GeneRIF: Lpin-1 (H37A05.1) was required for disassembly of the nuclear lamina during late nuclear enveloppe breakdown
• Inactivation of the C. elegans lipin homolog leads to ER disorganization and to defects in the breakdown and reassembly of the nuclear envelope.
  Golden, Journal of cell science 2009
  • GeneRIF: study examines LPIN-1(H37A05.1) effect on nuclear envelope(NE)dynamics; lpin-1 RNAi causes defects in NE breakdown, abnormal chromosome segregation & nuclear morphology

Or search for genetic data about PF09949 in the Fitness Browser