Family Search for PF10728 (DUF2520)

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PF10728 hits 12 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.

3d1lB / Q5LAQ6 Crystal structure of putative NADP oxidoreductase bf3122 from bacteroides fragilis
Aligns to 131:256 / 259 (48.6%), covers 100.0% of PF10728, 142.0 bits

• Ligand: 2-mercapto-propion aldehyde (3d1lB)

PG0659 conserved hypothetical protein from Porphyromonas gingivalis W83
Aligns to 136:261 / 270 (46.7%), covers 100.0% of PF10728, 130.4 bits

• The Porphyromonas gingivalis Hybrid Cluster Protein Hcp Is Required for Growth with Nitrite and Survival with Host Cells
  Belvin, Infection and immunity 2019
  • “...groEL htpG dnaJ groES PG1811 PG2043 grpE DnaK PyrH PG0659 clpB PG1778 PG1618 PG1649 PG0975 PG0747 aroA aFld, bP fold change of transcript between conditions...”

DET0801 pyrroline-5-carboxylate reductase, putative from Dehalococcoides ethenogenes 195
Aligns to 132:261 / 285 (45.6%), covers 98.4% of PF10728, 127.2 bits

• Global transcriptomic and proteomic responses of Dehalococcoides ethenogenes strain 195 to fixed nitrogen limitation
  Lee, Applied and environmental microbiology 2012
  • “...DET0142 DET0143 DET0198 DET0253 DET0271 DET0275 DET0299 DET0801 DET0802 DET0803 DET0804 DET0813 DET0814 DET0871 DET0908 DET1005 DET1057 DET1101 DET1102 DET1103...”

CBU_1660 hypothetical protein from Coxiella burnetii RSA 493
Aligns to 138:266 / 294 (43.9%), covers 97.6% of PF10728, 118.2 bits

• PanG, a new ketopantoate reductase involved in pantothenate synthesis
  Miller, Journal of bacteriology 2013
  • “...493 PanG is annotated as hypothetical protein CBU_1660, Clostridium botulinum BKT015925 PanG is annotated as hypothetical protein CbC4_0183, and...”

EF1861 conserved domain protein from Enterococcus faecalis V583
Aligns to 132:258 / 281 (45.2%), covers 98.4% of PF10728, 116.9 bits

• High quality draft genome sequence of Corynebacterium ulceribovis type strain IMMIB-L1395(T) (DSM 45146(T))
  Yassin, Standards in genomic sciences 2015
  • “...Homologs of this KPR protein are present in other bacterisa such as Enterococcus faecalis V583 (EF1861), Francisella novicidia (FTT1388) and Clostridium difficile . The KPR protein has been shown to also catalyze the conversion of 2-dehydropantoate to pantoate in Francisella species [ 99 ]. In the...”
• PanG, a new ketopantoate reductase involved in pantothenate synthesis
  Miller, Journal of bacteriology 2013
  • “...Both the homologous gene from E. faecalis V583 (EF1861) and E. coli panE functionally complemented Francisella novicida lacking any KPR. Furthermore, panG from...”
  • “...V583 PanG is annotated as hypothetical protein EF1861, Clostridium difficile 630 PanG is annotated as hypothetical protein CD630-15140, Coxiella burnetii RSA...”

2i76A / Q9X250 Crystal structure of protein tm1727 from thermotoga maritima
Aligns to 112:240 / 247 (52.2%), covers 88.0% of PF10728, 108.9 bits

• Ligand: nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (2i76A)

MAB_0540 hypothetical protein from Mycobacterium abscessus ATCC 19977
Aligns to 164:306 / 326 (43.9%), covers 98.4% of PF10728, 107.9 bits

• Epidemiology of Nontuberculous Mycobacteria in Nanjing and MAB_0540 Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus
  Zhang, Infection and drug resistance 2023
  • “...Resistance 1178-6973 Dove 10171220 408986 10.2147/IDR.S408986 Original Research Epidemiology of Nontuberculous Mycobacteria in Nanjing and MAB_0540 Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus Zhang et al Zhang et al Zhang Ruixian 1 Luo Sha 1 Wang Nan 1 Zhang Hongying 2 http://orcid.org/0000-0002-4896-0209 Wu Xuping 1...”
  • “...of M. abscessus and M. intracellulare . Furthermore, we determined whether MAB_1448, MAB_4384, MAB_2299c, MAB_1483, MAB_0540, rplD, rplC , and rrl were related to drug resistance to provide an experimental basis for the use of these five drugs in the treatment of NTM. Methods We identified...”
• Nontuberculous Mycobacterial Resistance to Antibiotics and Disinfectants: Challenges Still Ahead
  Tarashi, BioMed research international 2022
  • “...Some mutations related to clofazimine resistance were also identified in M. abscessus , such as MAB_0540, MAB_2299c, and MAB_1483 [ 80 ]. M. intracellulare also showed some mutations in the mmpL5 gene. However, M. avium isolates, despite being clofazimine-resistant, did not show any related mutations [...”
• Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
  Dohál, Microorganisms 2021
  • “...of M. abscessus . Their results revealed several high-confidence gene mutations (in MAB_2299c, MAB_1483, and MAB_0540 genes) involved in resistance to this drug, whose use is increasingly preferred due to limited treatment options [ 77 ]. In addition, M. abscessus subsp. abscessus possesses the erm gene,...”
• Current Molecular Therapeutic Agents and Drug Candidates for Mycobacterium abscessus
  Quang, Frontiers in pharmacology 2021
  • “...using 29 laboratory-induced CFZ-resistant Mab strains through whole-genome sequencing, wherein three genes (MAB_2299c, MAB_1483 and MAB_0540) were identified to be most commonly associated with CFZ resistance. However, future studies are needed to address the role of the identified mutations ( Chen et al., 2018 ). CFZ...”
• Novel Mutations Associated with Clofazimine Resistance in Mycobacterium abscessus
  Chen, Antimicrobial agents and chemotherapy 2018
  • “...a possible transcriptional regulatory protein), MAB_1483, and MAB_0540 are most commonly associated with clofazimine resistance. In addition, mutations in...”
  • “...We conclude that mutations in MAB_2299c, MAB_1483, and MAB_0540 are the major mechanisms of clofazimine resistance in M. abscessus. Future studies are needed to...”
• Identification of novel mutations associated with clofazimine resistance inMycobacterium abscessus
  Chen, 2018

O06279 Conserved hypothetical alanine and leucine rich protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
BCG_3667c hypothetical alanine and leucine rich protein from Mycobacterium bovis BCG str. Pasteur 1173P2
Rv3603c CONSERVED HYPOTHETICAL ALANINE AND LEUCINE RICH PROTEIN from Mycobacterium tuberculosis H37Rv
Aligns to 145:287 / 303 (47.2%), covers 99.2% of PF10728, 107.3 bits

• A d-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis
  Pepi, ACS infectious diseases 2022
  • “..., 17 . C-terminally His-tagged variants of Mtb IlvC (UniProt: P9WKJ7) and Mtb PanG (UniProt: O06279) were produced from codon-optimized genes using pET28a(+) expression vectors (GenScript, Piscataway, NJ). The sequences are available in Supporting Table S4 . Mtb IlvC was expressed in E. coli BL21(DE3) and...”
• Mammalian lectin arrays for screening host-microbe interactions
  Jégouzo, The Journal of biological chemistry 2020
  • “...origin of replication, with a hygromycin-resistance cassette and a panCD operon driven by the constitutive BCG_3667c promoter, followed by the multiple-cloning site ( 60 ). Mycobacteria were grown at 37 C in Middlebrook 7H9 broth (BD Biosciences) supplemented with 0.05% Tween 80 (Sigma), 0.2% glycerol (Sigma),...”
• Analysis of Genome-Wide Mutational Dependence in Naturally Evolving Mycobacterium tuberculosis Populations
  Green, Molecular biology and evolution 2023
  • “...protein MoeB2 3894732 Rv3478 ppe60 Host immune response ( Su et al. 2018 ) 4046007 Rv3603c Rv3603c Conserved hypothetical alanine- and leucine-rich protein 4060588 Rv362 c esxW Influencing increased transmission in Beijing lineage ( Holt et al. 2018 ) 4338371 Rv3862c whiB6 Transcriptional regulator with known...”
• The Global Success of Mycobacterium tuberculosis Modern Beijing Family Is Driven by a Few Recently Emerged Strains
  Zhu, Microbiology spectrum 2023
  • “...4215793 A/C Rv3770c : Hypothetical leucine-rich protein Rv3770c : Probable remnant of a transposase L2.3.6 Rv3603c 4046007 C/T V38M Conserved hypothetical alanine- and leucine-rich protein DISCUSSION In this study, we used phylogenetic branching and population dynamics to demonstrate that L2.3 (modern Beijing) diversified into six phylogenetic...”
• A d-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis
  Pepi, ACS infectious diseases 2022
  • “...35015509 9558617 10.1021/acsinfecdis.1c00461 NIHMS1839722 Article A D-Phenylalanine-Benzoxazole Derivative Reveals the Role of the Essential Enzyme Rv3603c in the Pantothenate Biosynthetic Pathway of Mycobacterium tuberculosis Pepi Michael J. 1 Chacko Shibin 2 Marqus Gary M. 1 Singh Vinayak 3 Wang Zhe 4 Planck Kyle 4 Cullinane Ryan...”
  • “...glutamicum , but Mtb IlvC does not display KPR activity. We identified the essential protein Rv3603c as an ortholog of PanG KPR, and demonstrated that purified recombinant Rv3603c has KPR activity. Q112 inhibits Rv3603c, explaining the metabolomic changes. Surprisingly, pantothenate does not rescue Q112 -treated bacteria,...”
• Vitamin in the Crosshairs: Targeting Pantothenate and Coenzyme A Biosynthesis for New Antituberculosis Agents
  Butman, Frontiers in cellular and infection microbiology 2020
  • “...( Ec PanK). The other, Mt CoaX, is a type III PanK encoded by coaX (Rv3603c), which is found in an open reading frame with panD and panC . However, unlike other well-characterized type III PanKs such as the enzymes from Pseudomonas aeruginosa and Bacillus anthracis...”
• Comprehensive Characterization of the Attenuated Double Auxotroph Mycobacterium tuberculosisΔleuDΔpanCD as an Alternative to H37Rv
  Mouton, Frontiers in microbiology 2019
  • “...play a role in dormancy, oxidative and/or nitrosative stress (AhpC, AhpD), pantothenate metabolism (CoaX, PanB, Rv3603c), transcription (Rv2989), methyl transfer (Rv2003c) and lipid catabolism (Rv2037c). This, in addition to increased abundance of DevR in M. tuberculosis leuD panCD during growth at pH 6.5, may suggest that...”
• Mutations in panD encoding aspartate decarboxylase are associated with pyrazinamide resistance in Mycobacterium tuberculosis
  Zhang, Emerging microbes & infections 2013
  • “...the 5 individual mutants. panD is located in an operon lysS-Rv3603c with panC (pantothenate synthetase), Rv3603c (conserved hypothetical alanine and leucine rich protein), Rv3600c (hypothetical protein), Rv3559c (hypothetical protein ), and lysS (lysyl-tRNA synthetase 1) ( Figure 2 ). panD encodes a 139 amino acid (15kD)...”
• Annotation of the M. tuberculosis hypothetical orfeome: adding functional information to more than half of the uncharacterized proteins
  Doerks, PloS one 2012
  • “...(A) Species tree with family representatives and corresponding operon architecture. COG4595 (containing the hypothetical gene rv3603c (red unit)) evidentially linked to several genes of pantothenate (vitamine B5) biosynthesis pathway (variably colored units upstream the red unit) and to folic acid (vitamine B9) biosynthesis pathway (downstream the...”
  • “...synthetase is an attractive chemotherapeutic target [36] . In our genomic context analysis we detect rv3603c (member of COG5495), a gene of unknown function, in a conserved operon with panC and other genes of the pantothenate biosynthesis pathway ( Figure 4a and b ). Interestingly, the...”
• Exploring drug action on Mycobacterium tuberculosis using affymetrix oligonucleotide genechips
  Fu, Tuberculosis (Edinburgh, Scotland) 2006
  • “...0.001927 Rv3362c Probable ATP/GTP-binding protein 2.3 0.000122 Rv3603c Conserved hypothetical alanine and leucine rich protein 3.5 0.000429 Table 3 Functional...”

A9762_03120 DUF2520 domain-containing protein from Pandoraea sp. ISTKB
Aligns to 146:275 / 310 (41.9%), covers 96.8% of PF10728, 102.7 bits

• Genomic and proteomic analysis of lignin degrading and polyhydroxyalkanoate accumulating β-proteobacterium Pandoraea sp. ISTKB
  Kumar, Biotechnology for biofuels 2018
  • “...26.21 1.99E100 A0A1E3LAC9 A9762_09275 NADH oxidoreductase (quinone) subunit F 0.86275 19 70.7 47.093 5.72E135 A0A1E3LMK8 A9762_03120 NADP oxidoreductase 0.82498 10 55.5 32.458 3.02E38 A0A1E3LHK5 A9762_23470 FAD-dependent oxidoreductase 0.33833 3 14.7 38.458 4.28E15 A0A1E3LLW4 A9762_03220 GMC family oxidoreductase 0.24372 23 54.8 64.908 3.74E163 A0A1E3LFG3 A9762_22500 NADP-dependent oxidoreductase...”

FTN_1351 hypothetical protein from Francisella tularensis subsp. novicida U112
Aligns to 118:232 / 238 (48.3%), covers 85.6% of PF10728, 55.4 bits

• PanG, a new ketopantoate reductase involved in pantothenate synthesis
  Miller, Journal of bacteriology 2013
  • “...pulmonary and systemic infections in mice. Inactivation of FTN_1351 (panG) or FTN_1355 Received 24 September 2012 Accepted 6 December 2012 Published ahead of...”
  • “...and panD, as well as the homologs of FTN_1351 (panG) and FTN_1355 (coaX) in strains grown within bone marrow-derived macrophages. This suggests that...”
• Genome-wide screen in Francisella novicida for genes required for pulmonary and systemic infection in mice
  Kraemer, Infection and immunity 2009
  • “...and a gene for a hypothetical protein (FTN_1351). Candidate virulence genes are enriched for unknown/hypothetical genes and genes involved in transcription....”
  • “...IMMUN. TABLE 1--Continued FTN Gene name FTN_1349 FTN_1351 FTN_1355 FTN_1415 FTN_1455 FTN_1459 FTN_1464 FTN_1467 FTN_1471 FTN_1507 FTN_1534 FTN_1536 fadA lepB...”
• Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development
  Pechous, Microbiology and molecular biology reviews : MMBR 2009
  • “...I In Ar Ar/In Tp Tp FTN_1349 FTN_1351 FTN_1355 FTN_1357 FTN_1362 FTN_1400 FTN_1403 FTN_1415 FTN_1417 FTN_1421 FTN_1423 FTN_1425c-FTN_1427c FTN_1432 FTN_1439...”
  • “...FTN_1440 FTN_1444 FTN_1455 FTN_1459 FTN_1464 FTN_1465 FTN_1349 FTN_1351 FTN_1355 recB FTN_1362 FTN_1400 flmF1 FTN_1415 manB wbtH wbtG wbtDEF wbtA fadA FTN_1440...”

FTL_0675 hypothetical protein from Francisella tularensis subsp. holarctica
FTS_0676 2-dehydropantoate 2-reductase PanG from Francisella tularensis subsp. holarctica FSC200
Aligns to 117:231 / 237 (48.5%), covers 85.6% of PF10728, 54.0 bits

• Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
  Horzempa, BMC microbiology 2008
  • “...46 , 69 ] FTL_0672 Aspartate-1-decarboxylase, panD 2.0 [ 14 , 46 , 69 ] FTL_0675 Conserved hypothetical protein 2.3 [ 46 , 69 ] FTL_0837 D-methionine binding transport protein, ABC transporter, membrane and periplasmic protein, metlQ 2.0 [ 13 ] FTL_0885 PhoH-like protein 1.9 [...”
• The Multiple Localized Glyceraldehyde-3-Phosphate Dehydrogenase Contributes to the Attenuation of the Francisella tularensis dsbA Deletion Mutant
  Pavkova, Frontiers in cellular and infection microbiology 2017
  • “...AFT92755 0920 Hypothetical protein FTS_0920 up- S Brotcke et al., 2006 AFT92560 0676 Hypothetical protein FTS_0676 up- S AFT92796 0974 Hypothetical protein FTS_0974 up- * S Ren et al., 2014 AFT92765 0935 GTP-dependent nucleic acid-binding protein EngD up- * J AFT92195 0200 Hypothetical protein FTS_0200 down-...”

panG / Q5NF59 2-dehydropantoate 2-reductase (EC 1.1.1.169) from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4) (see paper)
FTT_1388 hypothetical protein from Francisella tularensis subsp. tularensis SCHU S4
Aligns to 118:232 / 238 (48.3%), covers 85.6% of PF10728, 54.0 bits

• Solving a Bloody Mess: B-Vitamin Independent Metabolic Convergence among Gammaproteobacterial Obligate Endosymbionts from Blood-Feeding Arthropods and the Leech Haementeria officinalis
  Manzano-Marín, Genome biology and evolution 2015
  • “...tularensis subsp. tularensis , a hypothetical protein which can convert 2-dehyropantoate to pantoate (locus tag: FTT_1388), for whose gene they propose the name panG , was identified ( Miller et al. 2013 ). Therefore, panG could substitute the function of the panE gene in B 5...”

Or search for genetic data about PF10728 in the Fitness Browser