Family Search for PF10759 (BPA)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF10759 hits 6 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
BPA_MYCS2 / A0R5Z0 Bacterial proteasome activator from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
MSMEG_6365 hypothetical protein from Mycobacterium smegmatis str. MC2 155
Aligns to 19:175 / 175 (89.7%), covers 97.5% of PF10759, 269.5 bits
- function: Interacts with the core proteasome alpha-subunit (PrcA) through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif) (PubMed:25469515). Interaction of Bpa with the proteasome stimulates proteasomal peptidase and casein degradation activity, which suggests Bpa could play a role in the removal of non-native or damaged proteins by influencing the conformation of the proteasome complex upon interaction (By similarity).
subunit: Forms a homooligomeric, either hexameric or heptameric, ring- like structure which stacks co-axially with the proteasomal alpha- rings. - ATP-independent substrate recruitment to proteasomal degradation in mycobacteria
von, Life science alliance 2023 - “...2015 ) was previously shown to affect the expression of essential downstream genes. Because bpa (MSMEG_6365) in M. smegmatis is located upstream of the orthologous genes (MSMEG_6366, MSMEG_6367, MSMEG_6369) and they were shown to be essential in M. smegmatis ( Dragset et al, 2019 ), we...”
- Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...Functional category Log 2 FC MSMEG_1392 alcohol dehydrogenase, class IV Intermediary metabolism and respiration 2.2 MSMEG_6365 Rv3780 conserved hypothetical protein Conserved hypotheticals 2.1 MSMEG_5915 Rv3516 ( echA19 ) enoyl-CoA hydratase Lipid metabolism 1.2 MSMEG_1530 integral membrane protein Conserved hypotheticals 1.1 MSMEG_0408 ( pks ) type I...”
- Bacterial proteasome activator bpa (rv3780) is a novel ring-shaped interactor of the mycobacterial proteasome
Delley, PloS one 2014 - “...interaction using a bacterial adenylate cyclase two-hybrid system (BACTH, see next paragraph), the target proteins Msmeg_6365 ( Msm Bpa) corresponding to Rv3780 in Mtb and Msmeg_3894 ( Msm PrcA) were fused to the C-terminus of the catalytic adenylate cyclase domains T25 and T18, respectively. Both genes...”
- Investigation of ABC transporter from mycobacterial arabinogalactan biosynthetic cluster
Dianišková, General physiology and biophysics 2011 - “...thus suggests that they are co-transcribed. With only 7 bp separating MSMEG_6366 from the conserved MSMEG_6365 gene (orthologous to Rv3780 from M. tuberculosis ; 3 bp spacing in this species), it is possible that it also belongs to the same transcriptional unit. However, its function is...”
BPA_MYCTU / P9WKX3 Bacterial proteasome activator from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
Aligns to 24:174 / 174 (86.8%), covers 86.9% of PF10759, 263.7 bits
- function: Interacts with the core proteasome alpha-subunit (PrcA) through its C-terminal hydrophobic-tyrosine-X motif (HbYX motif). Interaction of Bpa with the proteasome stimulates proteasomal peptidase and casein degradation activity, which suggests Bpa could play a role in the removal of non-native or damaged proteins by influencing the conformation of the proteasome complex upon interaction. Can inhibit degradation of Pup-tagged substrates in vitro by competing with Mpa for association with the proteasome.
subunit: Forms a homooligomeric, either hexameric or heptameric, ring- like structure which stacks co-axially with the proteasomal alpha- rings. - The unfoldase ClpC1 of Mycobacterium tuberculosis regulates the expression of a distinct subset of proteins having intrinsically disordered termini
Lunge, The Journal of biological chemistry 2020 (secret)
NP_218297 hypothetical protein from Mycobacterium tuberculosis H37Rv
Rv3780 hypothetical protein from Mycobacterium tuberculosis H37Rv
Aligns to 28:178 / 178 (84.8%), covers 86.9% of PF10759, 263.6 bits
- An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis.
Jastrab, Proceedings of the National Academy of Sciences of the United States of America 2015 - GeneRIF: Rv3780 is an ATP-independent proteasome activator in Mycobacterium tuberculosis
- Bacterial proteasome activator bpa (rv3780) is a novel ring-shaped interactor of the mycobacterial proteasome.
Delley, PloS one 2014 - GeneRIF: Characterization of a novel bacterial proteasome activator of Mycobacterium tuberculosis, Rv3780.
- Comprehensive essentiality analysis of the Mycobacterium kansasii genome by saturation transposon mutagenesis and deep sequencing
Levendosky, mBio 2023 - “...prcB ( Rv2110c ) encoding the proteasome subunits PrcA and PrcB, respectively, as well as Rv3780 encoding the bacterial proteasome activator/accessory factor PafE, were identified as ES ( 35 ). Our essentiality analysis, which was carried out using the same solid medium as that of the...”
- Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...category Log 2 FC MSMEG_1392 alcohol dehydrogenase, class IV Intermediary metabolism and respiration 2.2 MSMEG_6365 Rv3780 conserved hypothetical protein Conserved hypotheticals 2.1 MSMEG_5915 Rv3516 ( echA19 ) enoyl-CoA hydratase Lipid metabolism 1.2 MSMEG_1530 integral membrane protein Conserved hypotheticals 1.1 MSMEG_0408 ( pks ) type I modular...”
- Survival in Hostile Conditions: Pupylation and the Proteasome in Actinobacterial Stress Response Pathways
von, Frontiers in molecular biosciences 2021 - “...which the PPS is tightly linked. The alternative ring shaped ATP- and pupylation-independent activator Bpa (Rv3780, light pink) is not found in the Pup-Proteasome gene locus, but strictly co-occurs with the proteasomal subunit genes across Actinobacteria. A subgroup of Actinobacteria is missing the proteasomal subunit genes...”
- “...Ziemski M. Bolten M. Boehringer D. Weber-Ban E. ( 2014 ). Bacterial Proteasome Activator Bpa (Rv3780) Is a Novel Ring-Shaped Interactor of the Mycobacterial Proteasome . PLoS One 9 ( 12 ), e114348 . 10.1371/journal.pone.0114348 25469515 Delley C. L. Mller A. U. Ziemski M. Weber-Ban E....”
- The unfoldase ClpC1 of Mycobacterium tuberculosis regulates the expression of a distinct subset of proteins having intrinsically disordered termini
Lunge, The Journal of biological chemistry 2020 (secret) - Targeting the Proteostasis Network for Mycobacterial Drug Discovery
Lupoli, ACS infectious diseases 2018 - “...protein substrates prior to entry into the proteasome. PafE (proteasome accessory factor E, encoded by rv3780 ), an ATP- and Pup-independent unfolding machine, associates with and activates proteolysis through the proteasome in Mtb. 116 , 117 Similar to Mpa, 118 PafE contains a C-terminal hydrophobic-tyrosine-X-motif (HbYX...”
- “...Bolten M. ; Boehringer D. ; Weber-Ban E. ( 2014 ) Bacterial proteasome activator Bpa (Rv3780) is a novel ring-shaped interactor of the mycobacterial proteasome . PLoS One 9 ( 12 ), e114348 10.1371/journal.pone.0114348 . 25469515 Wu Y. ; Hu K. ; Li D. ; Bai...”
- Bacterial Proteasomes: Mechanistic and Functional Insights
Becker, Microbiology and molecular biology reviews : MMBR 2017 - “...to the Pup proteasomal genes (pafE is annotated Rv3780 for M. tuberculosis). PafE assembles into an unprecedented dodecameric ring with 12-fold symmetry (Fig....”
- “...Weber-Ban E. 2014. Bacterial proteasome activator bpa (rv3780) is a novel ringshaped interactor of the mycobacterial proteasome. PLoS One 9:e114348....”
- Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis
Bai, Proceedings of the National Academy of Sciences of the United States of America 2016 - “...found that proteasome accessory factor E (PafE, Rv3780) activates proteolysis by the Mtb proteasome independently of adenosine triphosphate (ATP). Moreover,...”
- “...discovered that Mtb has an analogous factor encoded by Rv3780 that we call PafE (proteasome accessory factor E; also known as Bpa for bacterial proteasome...”
- PNAS Plus Significance Statements
, Proceedings of the National Academy of Sciences of the United States of America 2016 - More
MKAN_RS13390 bacterial proteasome activator family protein from Mycobacterium kansasii ATCC 12478
Aligns to 26:172 / 173 (85.0%), covers 83.1% of PF10759, 262.3 bits
- Comprehensive essentiality analysis of the Mycobacterium kansasii genome by saturation transposon mutagenesis and deep sequencing
Levendosky, mBio 2023 - “...identified MKAN_RS02165 and MKAN_RS02170 , encoding Mk PrcA and PrcB, respectively, as NE, while the MKAN_RS13390 , encoding Mk PafE, was identified as GA. Notably, however, none of the three Mk genes appear to have identifiable paralogs in the genome. Thus, our findings might suggest that...”
MAV_0239 hypothetical protein from Mycobacterium avium 104
Aligns to 19:168 / 169 (88.8%), covers 83.1% of PF10759, 260.6 bits
ML0115 conserved hypothetical protein from Mycobacterium leprae TN
Aligns to 21:174 / 174 (88.5%), covers 86.2% of PF10759, 256.1 bits
- Developmental biology of Streptomyces from the perspective of 100 actinobacterial genome sequences
Chandra, FEMS microbiology reviews 2014 - “...), 3030 ( ML0762 ), 3576 ( Lxx03620 ), 3647 ( ML0284 ), 3822 ( ML0115 ), 3902 ( ML2687 ), 4043 ( Tfu_0030 ), 4287 ( ML1927 ), 4579 ( ML2064 ), 4590 ( Tfu_1240 ), 5145 ( ML1067) , 5167 ( Tfu_0515 ), 5173...”
Or search for genetic data about PF10759 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory