Family Search for PF11847 (DUF3367)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF11847 hits 6 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
AFTD_MYCS2 / A0QPD4 Alpha-(1->3)-arabinofuranosyltransferase; Arabinofuranan 3-O-arabinosyltransferase; EC 2.4.2.47 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
MSMEG_0359 hypothetical protein from Mycobacterium smegmatis str. MC2 155
Aligns to 5:685 / 1414 (48.2%), covers 99.5% of PF11847, 956.2 bits
- function: Involved in the biosynthesis of the arabinogalactan (AG) region of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the addition of an arabinofuranosyl (Araf) residue from the sugar donor decaprenyl-phospho-arabinose (DPA) on the C-3 of an alpha-(1->5)-linked Araf from the arabinan backbone of AG.
catalytic activity: Adds an alpha-D-arabinofuranosyl group from trans,octacis- decaprenylphospho-beta-D-arabinofuranose at the 3-O-position of an alpha-(1->5)-arabinofuranan chain attached to a beta-(1->5)- galactofuranan chain.
disruption phenotype: Cells lacking this gene show cell death, a decrease in its activity, defects in cell division, reduced growth, alteration of colonial morphology, and accumulation of trehalose dimycolates in the cell envelope. - AftD, a novel essential arabinofuranosyltransferase from mycobacteria
Skovierová, Glycobiology 2009 - “...nonpathogenic species, M. smegmatis, by allelic replacement. MSMEG_0359 encodes a 1414-amino-acid protein which is about 70% identical (80% similar) to the...”
- “...taken in constructing the disrupted copy of the MSMEG_0359 gene to delete the entire GT-C motif. Essentially the same strategy with the...”
Rv0236c PROBABLE CONSERVED TRANSMEMBRANE PROTEIN from Mycobacterium tuberculosis H37Rv
Aligns to 15:693 / 1400 (48.5%), covers 99.8% of PF11847, 934.4 bits
- Anti-tuberculosis drug development via targeting the cell envelope of Mycobacterium tuberculosis
Xu, Frontiers in microbiology 2022 - “...linear arabinan of both l LAM and AG ( Zhang et al., 2011 ). AftD (Rv0236c) is the largest glycosyltransferase in the genome of M . tuberculosis . Glycan array analysis shows that AftD binds to complex arabinose glycans ( Figure 1D ; Tan et al.,...”
- Characterization of Drug-Resistant Lipid-Dependent Differentially Detectable Mycobacterium tuberculosis
Mesman, Journal of clinical medicine 2021 - “...Unknown - 6 Rv1721c VapB12 D48E non-synonymous Possible antitoxin VapB12 virulence, detoxification, adaptation 0 6 Rv0236c c aftD F21L non-synonymous Possible arabinofuranosyltransferase AftD cell wall and cell processes 0 a Amino acid substitutions are identified in reference to the consensus sequence of the H37RV strain. b...”
- Antibiotics and resistance: the two-sided coin of the mycobacterial cell wall
Batt, Cell surface (Amsterdam, Netherlands) 2020 - “...branch points in the AG are introduced by AftC (Rv2673) and possibly also by AftD (Rv0236c), which incorporate (13) linked Ara f residues ( Birch et al., 2008 , kovierov et al., 2009 ). Addition of these branches, followed by extensions, leads to a highly branched...”
- “...Ara f residues ( Birch et al., 2008 ). It has been postulated that AftD (Rv0236c) initiates further (13) branching, although this is unconfirmed ( kovierov et al., 2009 ). AftB (Rv3805c) terminates the arabinan with (12) linked Ara f ( Jankute et al., 2017 )....”
- The thick waxy coat of mycobacteria, a protective layer against antibiotics and the host's immune system
Batt, The Biochemical journal 2020 - “...3,5-arabinose branches in both LAM [ 217 ] and AG synthesis [ 218 ]. AftD (Rv0236c) has been shown to have similar activity to AftC and may also introduce branch points [ 219 ], though (15) activity in AG synthesis has also been demonstrated [ 220...”
- In Vivo Methods to Study Protein-Protein Interactions as Key Players in Mycobacterium Tuberculosis Virulence
Veyron-Churlet, Pathogens (Basel, Switzerland) 2019 - “...acid biosynthesis (MmpL11 and Rv0228 = TmaT), peptidoglycanbiosynthesis (Rv3909, Rv3910 and Rv1337), glycolipid biosynthesis (Rv0227c, Rv0236c = AftD and Rv1457c), and cell division (CrgA) [ 67 ]. 3.2.4. Mtb Virulence Factors The BACTH system was used to search for partners of the virulence-associated factor Erp, which...”
- The MmpL3 interactome reveals a complex crosstalk between cell envelope biosynthesis and cell elongation and division in mycobacteria
Belardinelli, Scientific reports 2019 - “...TMS) TMM acetylation mycobacteria-conserved essential in Msmg ; predicted essential in Mtb 15 , 33 Rv0236c (AftD) IM protein (9 TMS) AG and LAM biosynthesis (arabinosyltransferase) mycobacteria-conserved essential in Msmg ; predicted essential in Mtb 41 , 42 Rv0625c IM protein (5 TMS) unknown absent from...”
- “...the elongation of lipomannan (LM) and lipoarabinomannan (LAM) in Corynebacterium glutamicum 40 , while AftD (Rv0236c) plays an essential role in the arabinosylation of LAM and arabinogalactan in mycobacteria 41 , 42 . Figure 4 Quantification of in vivo interactions between MmpL3 binding partners. CrgA, Wag31,...”
- Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex
Birhanu, Scientific reports 2019 - “...Capsule biosynthesis GlgM (Rv1212c), GlgB (Rv1326c), GlgE (Rv1327c), TreZ (Rv1562c) and MalQ (Rv1781c) AG AftD (Rv0236c), DprE1, EmbC and EmbR Lipoglycans (LM, LAM and PI) PimB (Rv2188c), EmbC, Rv1459c and Rv2181 Membrane transport proteins Sec SecA1, SecY, SecD, SecE2, SecF Tat TatB MmpL lipid transporters MmpL1,...”
- AftD functions as an α1 → 5 arabinofuranosyltransferase involved in the biosynthesis of the mycobacterial cell wall core
Alderwick, Cell surface (Amsterdam, Netherlands) 2018 - “...and strains The expression vectors made were pVWEx- Cg-aftD ( NCgl2757 ), pVWEx- Mt-aftD ( Rv0236c ) and the deletion vector pK19mobsacB aftD ( NCgl2757 ) with the gene number of the C. glutamicum and M. tuberculosis aftD orthologs added in parentheses. To enable in-frame deletion...”
- “...1997 ). Results Genome comparison of the aftD locus An in silico analysis of the Rv0236c open reading frame from M. tuberculosis as well as its ortholog ( NCgl2757 ) from C. glutamicum revealed a conserved syntenic region which is present in all species belonging to...”
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6w98A Single-particle cryo-em structure of arabinofuranosyltransferase aftd from mycobacteria (see paper)
2 alignments in 20:629 / 1312 (46.5%), covering up to 49.7% of PF11847, 787.7 bits
- Ligands: calcium ion; [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate; 4'-phosphopantetheine (6w98A)
6wbyA / A0A418KZ72 Single-particle cryo-em structure of arabinofuranosyltransferase aftd from mycobacteria, mutant r1389s class 2 (see paper)
2 alignments in 17:623 / 1250 (45.7%), covering up to 49.4% of PF11847, 766.7 bits
- Ligand: calcium ion (6wbyA)
NCgl2757 DUF3367 domain-containing protein from Corynebacterium glutamicum ATCC 13032
Aligns to 25:631 / 1035 (58.6%), covers 99.5% of PF11847, 686.7 bits
cg3161 hypothetical protein from Corynebacterium glutamicum ATCC 13032
Aligns to 1:603 / 1007 (59.9%), covers 98.9% of PF11847, 684.8 bits
- Genome-wide identification of novel genes involved in Corynebacteriales cell envelope biogenesis using Corynebacterium glutamicum as a model
de, PloS one 2020 - “...2: cg3157 (HsecP), 3: cg3158 (NagA2: putative -N-acetylglucosaminidase), 4: cg3159 (UspA), 5: cg3160 (HsecP), 6: cg3161 (AftD, arabinosyltransferase, AG biosynthesis), 7: cg3162 (HP), 8: cg3163 (TmaT, TMCM mycolylacetyltransferase), 9: cg3164 (HMP), 10: cg3165 (HMP), 11: cg3166 (HP, putative glycosyltransferase), 12: cg3167 (HP), 13: cg3168 (MtrP, methyltransferase),...”
- “...TMM (to give TDM) and AG [ 15 , 16 ] Arabinogalactan biosynthesis aftD (ort- cg3161 ) 2 AftD: arabinosyltransferase D involved in the biosynthesis of the arabinan domain of AG [ 48 ] aftB (ort- cg3187 ) 1 AftB: arabinosyltransferase B involved in the biosynthesis...”
Or search for genetic data about PF11847 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory