Family Search for PF11896 (GlgE_dom_N_S)
April 2024: See Interactive Tools for Functional Annotation of Bacterial Genomes for advice on using these tools.
PF11896 hits 44 sequences in PaperBLAST's database above the trusted cutoff. Showing all hits. Or show only hits to curated sequences or try another family.
Rxyl_0314 alpha amylase, catalytic region from Rubrobacter xylanophilus DSM 9941
Aligns to 6:186 / 646 (28.0%), covers 98.9% of PF11896, 219.4 bits
SSGG_05058 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Streptomyces filamentosus NRRL 15998
Aligns to 3:190 / 672 (28.0%), covers 100.0% of PF11896, 219.2 bits
- Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus
Liao, Microbial cell factories 2013 - “...catalyzes the conversion of maltose into Trehalose was elevated. In addition, gene expression of alpha-amylase (SSGG_05058) was induced, which degrade starch to provide the maltose for synthesis of trehalose. The accumulation of this nonreducing sugar has been previously viewed as an important osmoprotectant and stress protectant...”
- “...description Log 2 stress/control SSGG_01377 putative maltose ABC transporter permease 2.46 SSGG_05057 trehalose synthase 3.09 SSGG_05058 alpha-amylase 2.12 SSGG_03685 fructose-bisphosphate aldolase 1.37 SSGG_06343 phosphopyruvate hydratase 1.63 SSGG_01114 pyruvate kinase 1.30 SSGG_02477 phosphopyruvate hydratase 1.53 Genes encoding proteins involved in the TCA cycle were nearly unchanged. Interestingly,...”
4cn1A / Q9L1K2 Glge isoform 1 from streptomyces coelicolor d394a mutant with maltose- 1-phosphate bound (see paper)
Aligns to 5:192 / 649 (29.0%), covers 100.0% of PF11896, 214.8 bits
- Ligands: 1-o-phosphono-alpha-d-glucopyranose; alpha-d-glucopyranose (4cn1A)
GLGE1_STRCO / Q9L1K2 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 1; GMPMT 1; (1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase 1; EC 2.4.99.16 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
Q9L1K2 starch synthase (maltosyl-transferring) (EC 2.4.99.16) from Streptomyces coelicolor (see 3 papers)
SCO5443 alpha-amylase from Streptomyces coelicolor A3(2)
Aligns to 19:206 / 675 (27.9%), covers 100.0% of PF11896, 214.7 bits
- function: Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE- catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Alpha-D-glucose 1-phosphate cannot serve as a donor substrate, but alpha-maltosyl fluoride is an efficient donor in vitro. Exhibits an alpha-retaining catalytic mechanism, with evidence that maltooligosaccharide acceptors are extended at their non-reducing ends. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen or maltoheptaose in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
catalytic activity: [(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate (RHEA:42692)
subunit: Homodimer. - Quantitative Proteome and Phosphoproteome Analyses of Streptomyces coelicolor Reveal Proteins and Phosphoproteins Modulating Differentiation and Secondary Metabolism
Rioseras, Molecular & cellular proteomics : MCP 2018 - “...4 Proteasome protein - 1.9 - 3.7 SCO1646 n.s. Proteasome protein - 2.2 - 4.7 SCO5443 5 Alpha-amylase n.s. 2.2 n.s. 4.7 SCO6411 5 Hydrolase - 2 - 4 SCO6414 4 Hydrolase 1.2 2.3 2.3 4.9 1 For cell division proteins, variations inside the 1 interval...”
- Development of Series of Affinity Tags in Streptomyces
Mao, Scientific reports 2017 - “...13 0.9149 1 SCO3902, hypothetical protein 14 0.9107 1 SCO2295, hypothetical protein 15 0.9009 1 SCO5443, alpha-amylase 16 0.8982 1 SCO2086, UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase, MurD 17 0.8935 1 SCO2935, transcriptional regulator 18 0.8911 1 SCO1849, cobalamin biosynthesis protein, or cobaltochelatase subunit CobN 19 0.8785 1 SCO3857, regulatory...”
- Mycobacterium tuberculosis maltosyltransferase GlgE, a genetically validated antituberculosis target, is negatively regulated by Ser/Thr phosphorylation
Leiba, The Journal of biological chemistry 2013 - “...February 12, 2017 form I gene from S. coelicolor (Sco5443) was amplified using S. coelicolor M145 chromosomal DNA as the template with the forward and reverse...”
- Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum
Ventura, Microbiology and molecular biology reviews : MMBR 2007 - “...The most studied involves two nearly identical clusters (SCO5443 to -5440 and SCO7335 to -7332) that contain genes encoding functions involved in carbon storage...”
- “...paralogous members of the bldB (SCO5723) and whiJ (SCO5443) families (65). Based on the distribution of these developmental genes among actinobacterial genomes,...”
SVEN_5097 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Streptomyces venezuelae ATCC 10712
Aligns to 2:190 / 661 (28.6%), covers 100.0% of PF11896, 214.5 bits
F2R411 starch synthase (maltosyl-transferring) (EC 2.4.99.16) from Streptomyces venezuelae (see paper)
Aligns to 33:221 / 692 (27.3%), covers 100.0% of PF11896, 214.3 bits
GLGE2_STRCO / Q9KY04 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase 2; GMPMT 2; (1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase 2; EC 2.4.99.16 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
SCO7335 alpha-amylase from Streptomyces coelicolor A3(2)
Aligns to 10:197 / 669 (28.1%), covers 100.0% of PF11896, 213.6 bits
- function: Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP6 being optimal in the GlgE- catalyzed polymerization with M1P. Is probably involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
catalytic activity: [(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate (RHEA:42692)
subunit: Homodimer. - Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics
Dulermo, Microorganisms 2023 - “...of the total lipid content of S. coelicolor M145 (manuscript in preparation). 3.2.5. Deletion of sco7335 Encoding a Putative Amylase Sco7335 is located at the beginning of an operon involved in trehalose biosynthesis [ 38 ]. The deletion of this gene might impair the expression of...”
- Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum
Ventura, Microbiology and molecular biology reviews : MMBR 2007 - “...two nearly identical clusters (SCO5443 to -5440 and SCO7335 to -7332) that contain genes encoding functions involved in carbon storage transactions, with each...”
AOT42_02730 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Corynebacterium diphtheriae bv. gravis
Aligns to 6:197 / 678 (28.3%), covers 99.4% of PF11896, 194.6 bits
AO271_03420 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Corynebacterium diphtheriae
Aligns to 6:197 / 678 (28.3%), covers 99.4% of PF11896, 194.5 bits
DIP1066 Putative alpha-amylase (glucanase) from Corynebacterium diphtheriae NCTC 13129
Aligns to 6:197 / 678 (28.3%), covers 99.4% of PF11896, 194.0 bits
YP_701424 probable alpha amylase from Rhodococcus sp. RHA1
Aligns to 3:195 / 672 (28.7%), covers 100.0% of PF11896, 193.7 bits
5cimB / G7CL00 Structure of mycobacterium thermoresistibile glge in complex with maltose (cocrystallisation with maltose-1-phosphate) at 3.32a resolution (see paper)
Aligns to 4:200 / 675 (29.2%), covers 100.0% of PF11896, 193.4 bits
- Ligand: alpha-d-glucopyranose (5cimB)
PP_4060 alpha-amylase family protein from Pseudomonas putida KT2440
Aligns to 19:201 / 661 (27.7%), covers 100.0% of PF11896, 187.5 bits
WP_003896313 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Mycolicibacterium smegmatis MC2 155
MSMEG_4916 alpha-amylase family protein from Mycobacterium smegmatis str. MC2 155
Aligns to 3:217 / 692 (31.1%), covers 100.0% of PF11896, 187.3 bits
- A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of α-maltose-1-phosphate.
Syson, Biochimica et biophysica acta. General subjects 2021 - GeneRIF: A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of alpha-maltose-1-phosphate.
- Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...Differentially abundant proteins in uspC. Gene name Rv homologue Product Functional category Log 2 FC MSMEG_4916 Rv1327c ( glgE ) 1,4-glucan:maltose-1-phosphate maltosyltransferase Intermediary metabolism and respiration 3.0 MSMEG_1392 alcohol dehydrogenase, class IV Intermediary metabolism and respiration 2.9 MSMEG_0684 aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding Intermediary...”
- “...to class IV. We also observed an~8-fold increase in the abundance of the maltosyltransferase GlgE (MSMEG_4916) in uspC and~3.6-fold increase in uspABC. GlgE is essential in Mtb and uses maltose-1-phosphate (maltose-1P) donor to synthesise -glucan ( Kalscheuer et al., 2010a ). It is interesting to note...”
- A genomic view of sugar transport in Mycobacterium smegmatis and Mycobacterium tuberculosis
Titgemeyer, Journal of bacteriology 2007 - “...M. smegmatis has six homologs (msmeg_3184, msmeg_3576, msmeg_4916, msmeg_4917, msmeg_4696, and msmeg_6515) of MalL of B. subtilis, which hydrolyzes maltose,...”
GLGE_MYCS2 / Q9RP48 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase; GMPMT; (1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase; EC 2.4.99.16 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 3 papers)
Q9RP48 starch synthase (maltosyl-transferring) (EC 2.4.99.16) from Mycolicibacterium smegmatis (see paper)
Aligns to 8:222 / 697 (30.8%), covers 100.0% of PF11896, 187.3 bits
- function: Maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)-glucans. Is also able to catalyze the reverse reaction in vitro. Cannot use glucose 1-phosphate as substrate. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
catalytic activity: [(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate (RHEA:42692)
subunit: Homodimer.
disruption phenotype: Cells lacking this gene display M1P accumulation and trehalose sensitivity. These phenotypes are suppressed in mutants lacking both glgE and treS or both glgE and mak. Deletion of glgE does not increase glycogen content.
Hsero_2325 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Herbaspirillum seropedicae SmR1
Aligns to 444:649 / 1132 (18.2%), covers 100.0% of PF11896, 187.2 bits
- Genome of Herbaspirillum seropedicae strain SmR1, a specialized diazotrophic endophyte of tropical grasses
Pedrosa, PLoS genetics 2011 - “..., coding a trehalose-6-phosphate phosphatase, and a glucoamylase gene. The other system involves an alpha-amylase (Hsero_2325), trehalose synthase (Hsero_2326), and a 1,4-alpha-glucan branching enzyme (Hsero_2327) and constitute an operon. Furthermore, four Na + (K + )/H + antiporter ( nhaA , nhaP , arsB and Hsero_3967)...”
MAP2433 hypothetical protein from Mycobacterium avium subsp. paratuberculosis str. k10
Aligns to 21:237 / 712 (30.5%), covers 100.0% of PF11896, 185.9 bits
4u33A / P9WQ16 Structure of mtb glge bound to maltose (see paper)
Aligns to 2:188 / 661 (28.3%), covers 100.0% of PF11896, 185.1 bits
- Ligand: alpha-d-glucopyranose (4u33A)
PSPTO2760, PSPTO_2760 alpha-amylase family protein from Pseudomonas syringae pv. tomato str. DC3000
Aligns to 31:213 / 672 (27.2%), covers 100.0% of PF11896, 185.0 bits
- Pseudomonas syringae addresses distinct environmental challenges during plant infection through the coordinated deployment of polysaccharides
Krishna, Journal of experimental botany 2022 - “...PSPTO1027 ) and wssC (PSPTO1028) deletion Lammertz et al. (2019) treS Strain with glgE ( PSPTO2760 ), treS ( PSPTO2761 ), and glgB ( PSPTO2762 ) deletion This study treY/Z Strain with glgA ( PSPTO3125 ), treZ ( PSPTO3126 ), malQ ( PSPTO3127 ), treY (...”
- Identification of the trehalose biosynthetic loci of Pseudomonas syringae and their contribution to fitness in the phyllosphere
Freeman, Environmental microbiology 2010 (PubMed)- “...orthologue PSPTO_2761 is flanked by an upstream gene, PSPTO_2760, that is predicted to encode an a-amylase family protein and a downstream gene, PSPTO_2762,...”
- “...trehalose synthesis than GlgB. In contrast, the gene PSPTO_2760 is predicted to encode a protein with an a-amylase catalytic domain, which is a domain...”
H16_B1557 maltotransferase domain-containing protein from Cupriavidus necator H16
H16_B1557 Glycosidase-like protein from Ralstonia eutropha H16
Aligns to 447:650 / 1138 (17.9%), covers 100.0% of PF11896, 183.7 bits
RHE_RS27870 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Rhizobium etli CFN 42
Aligns to 431:614 / 1075 (17.1%), covers 100.0% of PF11896, 183.6 bits
glgE / P9WQ17 starch synthase (maltosyl-transferring) monomer (EC 2.4.99.16) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
GLGE_MYCTU / P9WQ17 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase; GMPMT; (1->4)-alpha-D-glucan:maltose-1-phosphate alpha-D-maltosyltransferase; (1->4)-alpha-D-glucan:phosphate alpha-D-maltosyltransferase; EC 2.4.99.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
P9WQ17 starch synthase (maltosyl-transferring) (EC 2.4.99.16) from Mycobacterium tuberculosis (see 2 papers)
NP_215843 alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase from Mycobacterium tuberculosis H37Rv
Rv1327c PROBABLE GLUCANASE GLGE from Mycobacterium tuberculosis H37Rv
Aligns to 16:226 / 701 (30.1%), covers 100.0% of PF11896, 181.9 bits
- function: Essential maltosyltransferase that uses maltose 1-phosphate (M1P) as the sugar donor to elongate linear or branched alpha-(1->4)- glucans. Maltooligosaccharides with a degree of polymerization (DP) superior or equal to 4 are efficient acceptors, with DP5 being optimal in the GlgE-catalyzed polymerization with M1P. Is specific for the alpha-anomer of M1P as substrate, since the beta-anomer of M1P gives no activity. Exhibits an alpha-retaining catalytic mechanism. Is also able to catalyze the reverse reaction in vitro, releasing M1P from glycogen in the presence of inorganic phosphate. Also catalyzes disproportionation reactions through maltosyl transfer between maltooligosaccharides. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, Mak and GlgB.
catalytic activity: [(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate = [(1->4)-alpha-D-glucosyl](n+2) + phosphate (RHEA:42692)
subunit: Homodimer.
disruption phenotype: GlgE inactivation causes rapid death of M.tuberculosis in vitro and in mice through a self-poisoning accumulation of maltose 1-phosphate, driven by a self-amplifying feedback stress response. - The unfoldase ClpC1 of Mycobacterium tuberculosis regulates the expression of a distinct subset of proteins having intrinsically disordered termini
Lunge, The Journal of biological chemistry 2020 (secret) - Systematic Analysis of Metabolic Pathway Distributions of Bacterial Energy Reserves
Wang, G3 (Bethesda, Md.) 2019 - “...Mycobacterium tuberculosis pep2 Maltokinase 455 Q7DAF6 ! PF18085 Mycobacterium tuberculosis glgE Alpha-1,4-glucan: maltose-1-phosphate Maltosyltransferase 701 P9WQ17 ! PF00128, PF11896 Mycobacterium tuberculosis Rv3032 Glycogen synthase 414 P9WMY9 PF13439, PF00534 Cupriavidus necator phaA Acetyl-CoA acetyltransferase 246 P14611 PF02803, PF00108 Cupriavidus necator phaB Acetoacetyl-CoA reductase 393 P14697 PF00106 Allochromatium...”
- Structural insight into Mycobacterium tuberculosis maltosyl transferase inhibitors: pharmacophore-based virtual screening, docking, and molecular dynamics simulations.
Sengupta, Journal of biomolecular structure & dynamics 2015 (PubMed)- GeneRIF: Studies show stable maltosyl transferasev protein ligand binding, indicating potential target for antitubercular therapeutic development.
- Crystal structures of Mycobacterium tuberculosis GlgE and complexes with non-covalent inhibitors.
Lindenberger, Scientific reports 2015 - GeneRIF: Crystal structures of Mycobacterium tuberculosis GlgE and complexes with non-covalent inhibitors.
- Modeling of a new tubercular maltosyl transferase, GlgE, study of its binding sites and virtual screening.
Sengupta, Molecular biology reports 2014 (PubMed)- GeneRIF: This reveals for the first time the unique 3D structure of mtb GlgE and provides insights into its active sites and substrate binding affinities.
- Mycobacterium tuberculosis maltosyltransferase GlgE, a genetically validated antituberculosis target, is negatively regulated by Ser/Thr phosphorylation.
Leiba, The Journal of biological chemistry 2013 - GeneRIF: GlgE pathway appears to be negatively regulated in actinomycetes through the phosphorylation of GlgE by PknB, a mechanism distinct from that known in the classical glycogen pathway.
- Structure of Streptomyces maltosyltransferase GlgE, a homologue of a genetically validated anti-tuberculosis target.
Syson, The Journal of biological chemistry 2011 - GeneRIF: analysis of GlgE from Streptomyces and Mycobacterium tuberculosis
- Immunogenicity of Mycobacterial Extracellular Vesicles Isolated From Host-Related Conditions Informs About Tuberculosis Disease Status
Schirmer, Frontiers in microbiology 2022 - “...Rv2215 DlaT 0.88 Rv1872c LldD2 1.33 Rv1023 Eno 0.81 Rv3841 BfrB 1.32 Rv3841 BfrB 0.75 Rv1327c GlgE 1.16 Rv3248c SahH 0.74 Rv1133c MetE 1.1 Unknown 1 Rv2818c Hypothetical protein 0.02 0 Regulatory proteins 19 Rv1479 MoxR1 0.16 2 Rv1479 MoxR1 0.26 Rv3295 probably TetR-family 0.11 Rv0144...”
- Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...abundant proteins in uspC. Gene name Rv homologue Product Functional category Log 2 FC MSMEG_4916 Rv1327c ( glgE ) 1,4-glucan:maltose-1-phosphate maltosyltransferase Intermediary metabolism and respiration 3.0 MSMEG_1392 alcohol dehydrogenase, class IV Intermediary metabolism and respiration 2.9 MSMEG_0684 aldehyde oxidase and xanthine dehydrogenase, molybdopterin binding Intermediary metabolism...”
- Effects of Host, Sample, and in vitro Culture on Genomic Diversity of Pathogenic Mycobacteria
Shockey, Frontiers in genetics 2019 - “...( fusA1 ) 2 1 2 3 Rv1164 ( narI ) 2 1 2 5 Rv1327c ( glgE ) 2 1 0 2 Rv1630 ( rpsA ) 2 2 1 2 Rv0667 ( rpoB ) 2 3 1 4 Rv0668 ( rpoC ) 1 1 2...”
- “...rpsA (Rv1630), iron sulfur binding reductase Rv0338c, respiratory nitrate reductase narI (Rv1164), and maltosyltransferase glgE (Rv1327c). Genes in this grouping are annotated as either intermediary metabolism ( n = 3) or information pathways ( n = 5); all but one ( narI /Rv1164) is essential for...”
- Ample glycosylation in membrane and cell envelope proteins may explain the phenotypic diversity and virulence in the Mycobacterium tuberculosis complex
Birhanu, Scientific reports 2019 - “...LprQ, FtsW, MviN, GlmS, GlmM, DacB1 and Wag31 Capsule biosynthesis GlgM (Rv1212c), GlgB (Rv1326c), GlgE (Rv1327c), TreZ (Rv1562c) and MalQ (Rv1781c) AG AftD (Rv0236c), DprE1, EmbC and EmbR Lipoglycans (LM, LAM and PI) PimB (Rv2188c), EmbC, Rv1459c and Rv2181 Membrane transport proteins Sec SecA1, SecY, SecD,...”
- Promising Recent Strategies with Potential Clinical Translational Value to Combat Antibacterial Resistant Surge
Karmakar, Medicines (Basel, Switzerland) 2019 - “..., 213 , 214 , 215 ], and it has been identified that GlgE (GlgE, Rv1327c) is vital for in vitro growth in Mtb [ 216 ]. The importance of GlgE arises from its involvement in the biosynthesis of the -1,4-glucan 47 synthesis via transferring the...”
- Mapping and characterization of G-quadruplexes in Mycobacterium tuberculosis gene promoter regions
Perrone, Scientific reports 2017 - “...40 GGGG AAAT GGG TGAATTAC GG TT GG T GGG C GG TG TGCTCC GGG Rv1327c glgE 12 GGG TGTGATC GG ATACTA GGG T GGG TATC GGG Rv0851c 18 GGG TGACTGCCTGAAATA GGG TTGCGTGCT GT GG AC GGG TTTCCC GGG Rv3634c galE1 4 GGG CGACCC GA GG...”
- A multiple genome analysis of Mycobacterium tuberculosis reveals specific novel genes and mutations associated with pyrazinamide resistance
Sheen, BMC genomics 2017 - “...excluding the strains with critical mutations in pncA , were Rv2505c, Rv2777c, Rv0735, Rv0787A, Rv0994, Rv1327c, Rv1742, Rv2317, Rv2557, Rv2857c, Rv3362c, Rv3393, Rv3410c, Rv3767c, Rv2646, Rv0668 , and Rv0667 . Analysis of mutations in the special set of genes For each of the mutations identified as...”
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MT1369 alpha-amylase family protein from Mycobacterium tuberculosis CDC1551
Aligns to 52:262 / 737 (28.6%), covers 100.0% of PF11896, 181.8 bits
Psyr_2489 Alpha amylase, catalytic region from Pseudomonas syringae pv. syringae B728a
Aligns to 31:213 / 672 (27.2%), covers 100.0% of PF11896, 180.1 bits
HZ99_RS03680 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Pseudomonas fluorescens
Aligns to 17:199 / 657 (27.9%), covers 100.0% of PF11896, 179.3 bits
- Combined Transcriptome and Proteome Analysis of RpoS Regulon Reveals Its Role in Spoilage Potential of Pseudomonas fluorescens
Liu, Frontiers in microbiology 2019 - “...1,4-alpha-glucan branching protein GlgB 0.210 (1.57E-07) Down/ HZ99_RS03675 WP_038441404.1 Maltose alpha-D-glucosyltransferase TreS 0.298 (1.60E-05) Down/ HZ99_RS03680 WP_038441406.1 Alpha-1,4-glucanmaltose-1-phosphate maltosyltransferase GlgE 0.247 (6.95E-07) HZ99_RS10715 WP_038442912.1 Phosphoethanolamine transferase OpgE 0.237 (2.95E-02) 0.553 (1.08E-02) Down/Down HZ99_RS19405 WP_038445324.1 Maltodextrin phosphorylase GlgP 0.190 (1.31E-09) 0.553 (5.91E-03) Down/Down HZ99_RS23185 WP_038446334.1 Trehalose permease...”
blr6766 blr6766 from Bradyrhizobium japonicum USDA 110
Aligns to 15:193 / 648 (27.6%), covers 98.3% of PF11896, 178.3 bits
XAC0154 alpha-amylase from Xanthomonas axonopodis pv. citri str. 306
Aligns to 387:571 / 1038 (17.8%), covers 100.0% of PF11896, 178.3 bits
- The dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses
Piazza, Journal of experimental botany 2015 - “...resulting -M1P is the substrate for synthesis of linear -1,4-glucan chains by glucan synthase (glgE; XAC0154), which are then branched by a branching enzyme (glgB; XAC0156) to form an -1,6-branched -1,4 glucan similar to glycogen, which is used as a storage carbohydrate. The presence of genes...”
- The rsmA-like gene rsmA(Xoo) of Xanthomonas oryzae pv. oryzae regulates bacterial virulence and production of diffusible signal factor
Zhu, Molecular plant pathology 2011 - “...and XOO0175, the homologue of the Xac a-amylase gene XAC0154, were down-regulated in the rsmAXoo mutant. However, the expression of these genes did not differ...”
- “...which is a homologue of the Xac a-amylase gene XAC0154, was down-regulated in the rsmAXoo mutant. The xrvA mutant of Xoo showed significantly reduced virulence...”
BP1026B_I2056 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Burkholderia pseudomallei 1026b
Aligns to 466:652 / 1136 (16.5%), covers 100.0% of PF11896, 177.2 bits
BPSL2074 putative alpha-amylase-related protein from Burkholderia pseudomallei K96243
Aligns to 478:664 / 1148 (16.3%), covers 100.0% of PF11896, 177.0 bits
cg1382 putative alpha-amylase from Corynebacterium glutamicum ATCC 13032
Aligns to 3:194 / 675 (28.4%), covers 100.0% of PF11896, 174.8 bits
CAP2UW1_2662 alpha amylase catalytic region from Candidatus Accumulibacter phosphatis clade IIA str. UW-1
Aligns to 7:195 / 653 (28.9%), covers 100.0% of PF11896, 171.3 bits
- Ancestral genome reconstruction identifies the evolutionary basis for trait acquisition in polyphosphate accumulating bacteria
Oyserman, The ISME journal 2016 - “...distinctive carbon metabolism of the PAO phenotype, including: glycogen degradation (CAP2UW1_0254, CAP2UW1_0255, CAP2UW1_2663), glycolysis (CAP2UW1_21242127, CAP2UW1_2662, CAP2UW1_2666, CAP2UW1_2669, CAP2UW1_3196, CAP2UW1_0487, CAP2UW1_1890), PHB metabolism (phaCCAP2UW1_0143, CAP2UW1_3185, CAP2UW1_3191), pyruvate ferredoxin oxidoreductase (PFORCAP2UW1_25102512) and acetate activation to acetyl-CoA (CAP2UW1_1515, CAP2UW1_2035). Another prominent laterally derived set of genes is P...”
- “...annotations, 31 genes were highly expressed. These included glycogen degradation (CAP2UW1_0255, CAP2UW1_2663), glycolysis (CAP2UW1_2124, CAP2UW1_21262127, CAP2UW1_2662, CAP2UW1_2666, CAP2UW1_3196, CAP2UW1_0487), PHB metabolism (CAP2UW1_3185, CAP2UW1_3191), pyruvate ferredoxin oxidoreductase (PFORCAP2UW1_25102512), ferrous iron transport (FeoACAP2UW1_0420) and NADP/NADPH transhydrogenase (PntABCAP2UW1_4179CAP2UW1_4180) ( Supplementary Spreadsheet 4 , Sheet 1, Column J). Furthermore, the...”
PA2151 hypothetical protein from Pseudomonas aeruginosa PAO1
Aligns to 20:203 / 664 (27.7%), covers 100.0% of PF11896, 165.5 bits
- Discovery of coordinately regulated pathways that provide innate protection against interbacterial antagonism
Ting, eLife 2022 - “...PA2976 0.84 0.83 PA4260 0.84 0.69 PA4265 0.84 0.61 PA4270 0.84 1.04 PA1803 0.78 0.74 PA2151 0.78 0.77 PA3950 0.78 1.19 PA3001 0.73 1.10 PA3656 0.73 0.80 PA5173 0.73 0.76 PA5554 0.73 1.20 PA5556 0.73 2.05 Arc3A-VSV-G sample PA5554 2.92 4.81 PA5113 (Arc3B ) 2.22 >...”
- “...PA4761 0.70 0.44 PA4941 0.65 2.57 PA4265 0.63 0.46 PA3656 0.61 0.67 PA3794 0.61 N.D. PA2151 0.56 0.55 PA3821 0.56 N.D. * Proteins containing at least two peptides identified in a given immunoprecipitation sample are included. Arc3A and Arc3B are bolded. Value corresponds to the abundance...”
- Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function
Babic, PloS one 2021 - “...coverage, 3% disall. res.). I) Superimposition of a homology model of the putative glycoside hydrolase PA2151 with S . pneumoniae SpuA (PDB ID 2YA0, chain A, 100% conf., 17% seq. id., rmsd c = 0.54 , 94% coverage, 1.9% disall. res.). J) Superimposition of a homology...”
- “...be novel virulence factors of P . aeruginosa . Among them are three enzymes, PA2984, PA2151 and PA1640, with putative hydrolytic activities. The C-terminal part (residues 494736) of PA2984 was reliably modelled ( Fig 4H and S7 Table ) with phosphorylcholine esterase Pce from Streptococcus pneumoniae...”
- Trehalose and α-glucan mediate distinct abiotic stress responses in Pseudomonas aeruginosa
Woodcock, PLoS genetics 2021 - “...in this study. Gene name Gene number Predicted enzyme function Reaction catalysed EC number glgE PA2151 Maltosyltransferase Extension of -glucan using M1P as the donor 2.4.99.16 treS / pep2 PA2152 Trehalose-maltose isomerase/maltokinase Isomerisation between trehalose and maltose/phosphorylation of maltose to M1P using ATP 5.4.99.16/2.7.1.175 glgB PA2153...”
- Comparative systems biology analysis to study the mode of action of the isothiocyanate compound Iberin on Pseudomonas aeruginosa
Tan, Antimicrobial agents and chemotherapy 2014 - “...M) Tan et al. TABLE 3 (Continued) Gene PA2144 PA2151 PA2152 PA2153 PA2159 PA2160 PA2162 PA2163 PA2164 PA2167 PA2169 PA2170 PA2177 PA2193 PA2194 PA2195 PA2261...”
- Coexistence and within-host evolution of diversified lineages of hypermutable Pseudomonas aeruginosa in long-term cystic fibrosis infections
Feliziani, PLoS genetics 2014 - “...PA0454 Hypothetical, unclassified, unknown 6 2 4 PA0788 5 0 5 PA2077 5 1 4 PA2151 5 2 3 PA2635 5 1 4 PA3728 5 2 3 PA4735 5 3 2 PA4836 5 1 4 a The categories used for functional classification were as described in...”
- Analysis of the Pseudomonas aeruginosa regulon controlled by the sensor kinase KinB and sigma factor RpoN
Damron, Journal of bacteriology 2012 - “...PA2143 PA2144 PA2145 PA2146 PA2147 PA2148 PA2149 PA2150 PA2151 PA2152 PA2153 PA2154 PA2155 PA2156 PA2157 PA2158 PA2159 PA2160 PA2161 PA2162 PA2163 PA2164 PA2165...”
- A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence
Goldová, BMC genomics 2011 - “...6.01 0.011 HP 9.4 4.6 6.9 4.7 PA2150 5.76 0.000 CHP 5.5 2.4 2.9 2.4 PA2151 3.46 0.041 CHP 7.0 3.6 5.2 3.4 PA2156 2.40 0.001 CHP 3.6 2.4 2.4 2.4 PA2157 4.03 0.002 HP 6.2 3.4 4.2 3.8 PA2158 4.51 0.019 Pr. alcohol dehydrogenase (Zn-dependent)...”
- Effects of antibiotics on quorum sensing in Pseudomonas aeruginosa
Skindersoe, Antimicrobial agents and chemotherapy 2008 - “...PA2300 PA2302 PA2069 PA2137 PA2139 PA2141 PA2142 PA2143 PA2144 PA2146 PA2148 PA2149 PA2151 PA2153 PA2158 hcnA hcnB hcnC chiC glgB glgp C C A A A C C C C C C C D...”
- More
PA14_36740 putative alpha-amylase family protein from Pseudomonas aeruginosa UCBPP-PA14
Aligns to 20:203 / 664 (27.7%), covers 100.0% of PF11896, 164.8 bits
- Impact of Growth Rate on the Protein-mRNA Ratio in Pseudomonas aeruginosa
Zhang, mBio 2023 - “...coxB , and PA14_01310 ), glycogen biosynthesis ( glgB , glgP , PA14_36730 , and PA14_36740 ), and sulfur metabolism ( PA14_19540 , ssuB , ssuD , and PA14_19590 ). FIG1 Differentially expressed mRNAs and proteins show modest overlap. Venn diagrams show the overlap between differentially...”
- Gene Expression Profiling of Pseudomonas aeruginosa Upon Exposure to Colistin and Tobramycin
Cianciulli, Frontiers in microbiology 2021 - “...25.56 6.53 glgB PA14_36710 Glycogen branching protein 38.34 15.21 PA14_36730 PA14_36730 Trehalose synthase 30.78 16.68 PA14_36740 PA14_36740 Hypothetical protein 4.77 5.04 glgP PA14_36840 Glycogen phosphorylase 5.98 2.93 PA14_36850 PA14_36850 Hypothetical protein 2.26 2.9 Pathogenicity and virulence tssL1 PA14_00925 Hypothetical protein 17.71 X tssk1 PA14_00940 Hypothetical protein...”
- Trehalose biosynthesis promotes Pseudomonas aeruginosa pathogenicity in plants
Djonović, PLoS pathogens 2013 - “...Besides the putative cellulase/peptidase, the PA14_3637536830 42.23 kb region encodes putative glucanolytic enzymes (PA14_36590, PA14_36630, PA14_36740) as well as two closely linked predicted operons ( http://www.pseudomonas.com ), PA14_36570-36630 consisting of six genes, and PA14_36710-37640 consisting of three genes, referred to hereafter as the treYZ and treS...”
- “...for trehalose synthesis. In addition to treS , the treS operon contains a predicted -amylase (PA14_36740), and glgB (PA14_36710), a predicted -1,4-branching enzyme ( Figure 1 ). 10.1371/journal.ppat.1003217.g001 Figure 1 Annotation of a 42.23 kb region of the P. aeruginosa PA14 genome encoding 38 genes (PA14_3637536830)...”
Q6L2Z8 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase from Picrophilus torridus (strain ATCC 700027 / DSM 9790 / JCM 10055 / NBRC 100828 / KAW 2/3)
Aligns to 2:183 / 630 (28.9%), covers 98.3% of PF11896, 155.4 bits
- Bioinformatics Analysis of Metabolism Pathways of Archaeal Energy Reserves
Wang, Scientific reports 2019 - “...pep2 Pep2 Maltokinase 455 Q7DAF6 / Picrophilus torridus A glgE GlgE Alpha-1,4-glucan: maltose-1-phosphate maltosyltransferase 630 Q6L2Z8 PF11896 PF00128 Mycobacterium tuberculosis B Rv3032 Rv3032 Glycogen synthase 414 P9WMY9 PF13439 PF00534 Polyhydroxyalkanoates (PHAs) Haloferax mediterranei A phaA PhaA Beta-ketothiolase 383 I3R3D1 PF00108 PF02803 Haloarcula hispanica A phaB PhaB...”
CPA40_RS03955 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Bifidobacterium callitrichos
Aligns to 87:294 / 762 (27.3%), covers 100.0% of PF11896, 151.9 bits
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...2.4.1.25 COO72_RS08655 CPA40_RS05200 BCAL_RS04695 Glycogen phosphorylase 2.4.1.1 COO72_RS08360 CPA40_RS09325 BCAL_RS09090 Starch synthase (maltosyl-transferring) 2.4.99.16 COO72_RS06885 CPA40_RS03955 BCAL_RS11605 Phosphoglucomutase (- d -glucose-1,6-bisphosphate-dependent) 5.4.2.2 COO72_RS10095 CPA40_RS03335 BCAL_RS04990 Protein-N-phosphohistidinecellobiose phosphotransferase 2.7.1.205 COO72_RS05245 CPA40_RS08235 BCAL_RS07995 Amino sugar and nucleotide sugar metabolism UDP- N -acetylmuramate dehydrogenase 1.3.1.98 COO72_RS09690 CPA40_RS03405 BCAL_RS04925 UDP-...”
BCAL_RS11605 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Bifidobacterium callitrichos DSM 23973
Aligns to 87:294 / 762 (27.3%), covers 100.0% of PF11896, 151.9 bits
- The comparative genomics of Bifidobacterium callitrichos reflects dietary carbohydrate utilization within the common marmoset gut
Albert, Microbial genomics 2018 - “...COO72_RS08655 CPA40_RS05200 BCAL_RS04695 Glycogen phosphorylase 2.4.1.1 COO72_RS08360 CPA40_RS09325 BCAL_RS09090 Starch synthase (maltosyl-transferring) 2.4.99.16 COO72_RS06885 CPA40_RS03955 BCAL_RS11605 Phosphoglucomutase (- d -glucose-1,6-bisphosphate-dependent) 5.4.2.2 COO72_RS10095 CPA40_RS03335 BCAL_RS04990 Protein-N-phosphohistidinecellobiose phosphotransferase 2.7.1.205 COO72_RS05245 CPA40_RS08235 BCAL_RS07995 Amino sugar and nucleotide sugar metabolism UDP- N -acetylmuramate dehydrogenase 1.3.1.98 COO72_RS09690 CPA40_RS03405 BCAL_RS04925 UDP- N...”
BAD_RS07575 maltotransferase domain-containing protein from Bifidobacterium adolescentis ATCC 15703
Aligns to 77:284 / 752 (27.7%), covers 100.0% of PF11896, 150.8 bits
BADO_1572 maltotransferase domain-containing protein from Bifidobacterium adolescentis
Aligns to 77:284 / 752 (27.7%), covers 100.0% of PF11896, 150.0 bits
- Development and Preliminary Validation of a Feasible Procedure for Isolating RNA from Fiber-Adherent Bacteria in Human Stool
Neff, Medical science monitor basic research 2019 - “...adolescentis : strains 22L, BBMN23, and ATCC 15703, genes measured: trpA (tryptophan synthase alpha chain), BADO_1572 (RS degrading enzyme). Eubacterium rectale : strains DSM17629, M104/1, ATCC 22656 , genes measured: trpB (tryptophan synthase beta chain), and amy13B (EUR_01860 RS degrader). All primers and probes were designed...”
- “...4 ). There was no effect of diet on expression of the fiber-degrading gene ( BADO_1572 ) Considering both starch conditions together, BADO_1572 exhibited increased expression in the adherent fraction in 9 of 11 samples (p=0.055), corresponding to a 53% increase ( Figure 4 ). TrpA...”
- Deciphering bifidobacterial-mediated metabolic interactions and their impact on gut microbiota by a multi-omics approach
Turroni, The ISME journal 2016 - “...BADO_1638, BADO_1639), one intracellular alpha-amylase (BADO_1572) and one extracellular pullulanase (BADO_0754) (Supplementary Figure S2). The...”
- Genomic characterization and transcriptional studies of the starch-utilizing strain Bifidobacterium adolescentis 22L
Duranti, Applied and environmental microbiology 2014 - “...as starch-derived glycans, including four proteins (BADO_1355, BADO_1572, BADO_1638, and BADO_1639) with predicted -amylase activity (Fig. 2b), which catalyzes...”
- “...and maltodextrin-induced genes that encode -amylase (BADO_1355, BADO_1572, and BADO_1639) as well as two predicted amylopullanases (BADO_0754 and BADO_1451),...”
AURMO_01094 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Aurantimicrobium photophilum
Aligns to 7:194 / 665 (28.3%), covers 100.0% of PF11896, 146.8 bits
BBMN68_1127 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Bifidobacterium longum subsp. longum BBMN68
Aligns to 72:279 / 746 (27.9%), covers 100.0% of PF11896, 142.6 bits
- Resistant starch utilization by Bifidobacterium, the beneficial human gut bacteria
Jung, Food science and biotechnology 2023 - “...pullulanase-like glycosidases (BBMN68_732, BBMN68_749, BBMN68_1610, and BBMN68_1127). The transcription of these genes in the predicted starch pathway revealed...”
- Starch and starch hydrolysates are favorable carbon sources for bifidobacteria in the human gut
Liu, BMC microbiology 2015 - “...starch degrading [ 18 ], were also found in the BBMN68 genome (BBMN68_732, BBMN68_749, BBMN68_1610, BBMN68_1127). However, genes encoding extracellular amylase were not found in the genome of BBMN68. It was speculated that BBMN68 cant directly utilize starch, since this strain does not produce an extracellular...”
Blon_0282 alpha amylase, catalytic region from Bifidobacterium longum subsp. infantis ATCC 15697
Aligns to 23:230 / 697 (29.8%), covers 100.0% of PF11896, 141.1 bits
Bbr_0296 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Bifidobacterium breve UCC2003
Aligns to 68:275 / 742 (28.0%), covers 100.0% of PF11896, 138.7 bits
BIF_01519 maltotransferase domain-containing protein from Bifidobacterium animalis subsp. lactis BB-12
Aligns to 51:258 / 731 (28.5%), covers 100.0% of PF11896, 136.4 bits
- Updated Genome Sequence for the Probiotic Bacterium Bifidobacterium animalis subsp. lactis BB-12
Jensen, Microbiology resource announcements 2021 - “...49bp66bp Intergenic(+77/+63) BIF_01176 / BIF_00941 395195 7bp19bp Intergenic(+120/+62) BIF_01176 / BIF_00941 412279 + CTGAGCACACGGGGGCCG Intergenic(+8/75) BIF_01519 / BIF_01115 412281 GT Intergenic(+10/73) BIF_01519 / BIF_01115 412292 +A Intergenic(+21/62) BIF_01519 / BIF_01115 412906 +54bp Intergenic(+25/+20) BIF_01115 / BIF_01114 416028 +C Intergenic(+371/+25) BIF_01112 / BIF_02188 416031 +GA Intergenic(+374/+22) BIF_01112...”
FSU_2442 alpha-1,4-glucan--maltose-1-phosphate maltosyltransferase from Fibrobacter succinogenes subsp. succinogenes S85
Fisuc_1932 alpha amylase catalytic region from Fibrobacter succinogenes subsp. succinogenes S85
Aligns to 9:100 / 576 (16.0%), covers 48.0% of PF11896, 91.5 bits
- Influence of Substrates on the Surface Characteristics and Membrane Proteome of Fibrobacter succinogenes S85
Raut, PloS one 2015 - “...10 - Unknown -0.336 67.554 5.69 Yes (2122) Proteins found only in cellulose treatments Fisuc_1932 FSU_2442 Alpha amylase catalytic region - - 2 GH13 Unknown -0.564 66.93 6.25 No - FSU_2932 Cadherin domain protein - 2 - - Outer membrane -0.422 317.91 4.69 Yes (2324) -...”
- A global analysis of gene expression in Fibrobacter succinogenes S85 grown on cellulose and soluble sugars at different growth rates
Neumann, Biotechnology for biofuels 2018 - “...phosphorylase), Fisuc_1530 (GH18), Fisuc_2097 (GT35, glycogen phosphorylase), Fisuc_1224 (GH5), Fisuc_3049 (GH2), Fisuc_1219 (GH8), Fisuc_0668 (GH57), Fisuc_1932 (GH13), and Fisuc_2988 (GH23) (Additional file 1 : Table S2). A clustered heatmap showing deviations from the mean r log expression across the samples for genes ( n =32) annotated...”
- Influence of Substrates on the Surface Characteristics and Membrane Proteome of Fibrobacter succinogenes S85
Raut, PloS one 2015 - “...20 10 - Unknown -0.336 67.554 5.69 Yes (2122) Proteins found only in cellulose treatments Fisuc_1932 FSU_2442 Alpha amylase catalytic region - - 2 GH13 Unknown -0.564 66.93 6.25 No - FSU_2932 Cadherin domain protein - 2 - - Outer membrane -0.422 317.91 4.69 Yes (2324)...”
- “...] localised on the surface of F . succinogenes . We also identified alpha amylase (Fisuc_1932) only in AS cellulose treatment, which may suggest partial conversion of cellulose to starch during the preparation of AS cellulose [ 52 ]. The 43 cell envelope proteins identified as...”
Or search for genetic data about PF11896 in the Fitness Browser
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory