PaperBLAST
PaperBLAST Hits for sp|B1V932|AMPA_PHYAS Probable cytosol aminopeptidase OS=Phytoplasma australiense OX=59748 GN=pepA PE=3 SV=1 (493 a.a., MKIYFKKNYL...)
Show query sequence
>sp|B1V932|AMPA_PHYAS Probable cytosol aminopeptidase OS=Phytoplasma australiense OX=59748 GN=pepA PE=3 SV=1
MKIYFKKNYLPSMDVDTAVVLQVEKYENSFGLEAVDPKGVAKKSFLRENFKGVFGTQVKL
LYPEGSPVACLQVMGLGKQEEINDQTFLKTGGLCFPQLNKANKVVVFADALGIENQTSQV
MHFALGLLLRSYSFKHYHTQKTKNEKNLEITFITENAELCQKEFDDVKAILGGVNLTKEL
VNEPANILGTNEFVERTQQLQTLGVEVEVLNKETLEKLGMNALLGVAQGSQRPPYLVVMK
WLGGNENEKPVAFVGKGVVFDTGGISLKPSNKMEDMKGDMAGAATVVGLMHALAARKAKV
NVLGVIGLVENMPGSNAQRPGDIVTSMSGQTIEVINTDAEGRLVLADALWYCKTKLQPKM
IIDLATLTGAIVVALGYEYAGLFSNNKELVKQLVHSGEVTEEKVWQFPLGPEYDRLVDGK
FADISNCPVGYGAGSITAAQFLKRFVGDDIPWAHIDIAGVASGKKKNEFNSSWASGFGVR
LLNHLVKDYYENK
Running BLASTp...
Found 221 similar proteins in the literature:
RHE_RS07430 leucyl aminopeptidase from Rhizobium etli CFN 42
55% identity, 96% coverage
- Rhizobium etli CFN42 proteomes showed isoenzymes in free-living and symbiosis with a different transcriptional regulation inferred from a transcriptional regulatory network
Taboada-Castro, Frontiers in microbiology 2022 - “...Bacteroid RHE_RS29645 bglX; beta-glucosidase [EC:3.2.1.21] K01255 MM RHE_RS01080 CARP, pepA; leucyl aminopeptidase [EC:3.4.11.1] K01255 Bacteroid RHE_RS07430 CARP, pepA; leucyl aminopeptidase [EC:3.4.11.1] K00405 MM RHE_RS29065 ccoO; cytochrome c oxidase cbb3-type subunit II K00405 Bacteroid RHE_RS30885 ccoO; cytochrome c oxidase cbb3-type subunit II K03412 MM RHE_RS03250 cheB; two-component...”
- “..., leucyl aminopeptidase [EC:3.4.11.1] enzyme, the RHE_RS01080 was expressed in MM, while in bacteroid the RHE_RS07430 was identified ( Table 1 ). For the gst , glutathione S-transferase [EC:2.5.1.18] in MM RHE_RS01425, RHE_RS05865, RHE_RS06130, RHE_RS06230, and RHE_RS11855 were identified and in bacteroids, RHE_RS05070, RHE_RS07560, RHE_RS25110, and...”
RL1571 putative aminopeptidase from Rhizobium leguminosarum bv. viciae 3841
53% identity, 99% coverage
BAB1_0710 Cytosol aminopeptidase:Aromatic amino acid hydroxylase from Brucella melitensis biovar Abortus 2308
Q2YN45 Probable cytosol aminopeptidase from Brucella abortus (strain 2308)
56% identity, 89% coverage
MexAM1_META1p3908 cytosol aminopeptidase (putative leucine aminopeptidase) from Methylobacterium extorquens AM1
MexAM1_META1p3908 leucyl aminopeptidase from Methylorubrum extorquens AM1
48% identity, 91% coverage
- Gene products and processes contributing to lanthanide homeostasis and methanol metabolism in Methylorubrum extorquens AM1
Roszczenko-Jasińska, Scientific reports 2020 - “...export MexAM1_META1p2734 ccmG c -type cytochrome biogenesis MexAM1_META1p2825 ccmB Heme export MexAM1_META1p2826 ccmA Heme export MexAM1_META1p3908 Leucyl aminopeptidase Table 2 Growth parameters for strains grown in methanol medium with La 3+ . Strain Growth rate (h 1 ) a in MeOH+La 3+ Growth of control strains...”
- “...MexAM1_META1p1771 0.110.01 orf6 0.030.00 orf7 9h lag, 0.080.01 MexAM1_META1p2359 No growth hss 6h lag, 0.100.01 MexAM1_META1p3908 15h lag, 0.070.00 Growth of strains lacking genes with known function pqqBCDE No growth pqqF No growth cycK No growth ccmB No growth ccmC No growth Growth of strains lacking...”
Rru_A0454 Leucyl aminopeptidase from Rhodospirillum rubrum ATCC 11170
47% identity, 98% coverage
- Integration of untargeted metabolomics with transcriptomics reveals active metabolic pathways
Cho, Metabolomics : Official journal of the Metabolomic Society 2014 - “...adduct can be metabolized through the detoxification process by -glutamyl transpeptidase (Rru_A0385) and leucyl aminopeptidase (Rru_A0454), which were not induced but constitutively expressed in our RNAseq experiment. Also, cysteine from the trans -sulfuration can be catabolized into pyruvate metabolism and the TCA cycle, as evidenced by...”
APH_0231 cytosol aminopeptidase from Anaplasma phagocytophilum HZ
49% identity, 87% coverage
CCNA_01763 cytosol aminopeptidase from Caulobacter crescentus NA1000
47% identity, 92% coverage
- Z-Ring-Associated Proteins Regulate Clustering of the Replication Terminus-Binding Protein ZapT in Caulobacter crescentus
Ozaki, mBio 2021 - “...( Fig.1B ). Moreover, binding of ZapT-3F to terminus-proximal regions such as the CCNA_01498, CCNA_01600, CCNA_01763, and CCNA_01936 loci ( Fig.1B , a, b, c, and d, respectively) was confirmed by quantitative PCR (ChIP-qPCR) ( Fig.1C ). The origin region (Cori) was used as a negative...”
AM956 cytosol aminopeptidase from Anaplasma marginale str. St. Maries
52% identity, 78% coverage
- Global transcriptional analysis reveals surface remodeling of Anaplasma marginale in the tick vector
Hammac, Parasites & vectors 2014 - “...RPKM Blood RPKM AM778 Outer membrane protein 9.8 4221.0 430.2 AM090 MSP4 6.7 5950.9 891.8 AM956 Cytosol aminopeptidase; pepA 6.6 1827.0 275.6 AM854 Putative peptidoglycan-associated lipoprotein 5.0 2672.7 532.8 AM097 VirB9-1 4.4 1925.6 434.9 AM197 Undefined product 3.9 54.9 14.2 AM1166 OMP5 3.8 828.7 216.5 AM560...”
- Genome-wide screening and identification of antigens for rickettsial vaccine development
Palmer, FEMS immunology and medical microbiology 2012 - “...+ A E W R AM854 + + + A E W O R N AM956 + A E W N R O AM1096 + A E W N R O Msp1a + 3 + Ac only Msp2 + + 4 A E W Msp3 +...”
- Identification of Anaplasma marginale proteins specifically upregulated during colonization of the tick vector
Ramabu, Infection and immunity 2010 - “...AM842 (dnaK), AM944 (groEL), AM254 (tuf), AM666 (atpD), AM956 (pepA), AM880 (alp2), AM564 (mdh), FIG. 2. Identification of Anaplasma marginale proteins uniquely...”
- Identification of novel antigenic proteins in a complex Anaplasma marginale outer membrane immunogen by mass spectrometry and genomic mapping
Lopez, Infection and immunity 2005 - “...factor Tu 5, 6, 2 18 433 Membrane associated 43 48 AM956 PepA cytosol amino peptidase 1 2 71 Outer membrane 49 49 AM254 AM1096 Elongation factor Tu OMA87 3 1 18...”
- “...15 (AM075, AM097, AM127, AM197, AM254, AM387, AM854, AM956, AM1096, AM1142, AM1164, AM1220, AM1223, AM1314, and AM1315) were recognized by IgG antibodies from...”
AOV_03525 leucyl aminopeptidase from Anaplasma ovis str. Haibei
52% identity, 83% coverage
- The Anaplasma ovis genome reveals a high proportion of pseudogenes
Liu, BMC genomics 2019 - “...X AOV_02195 trxB2 228 X AOV_02205 1023 AOV_02200 trxB2 669 X AOV_02415 pepA 1158 X AOV_03525 1497 AOV_02440 pepA 540 X AOV_02835 lpdA 420 X AOV_02845 1416 AOV_03085 ObgE 747 X AOV_02115 1044 AOV_03660 msp37 444 X AOV_03915 2426 AOV_04305 virB2a 342 X several \documentclass[12pt]{minimal} \usepackage{amsmath}...”
- “...[AOV_01020, AOV_01025] [AOV_02120 and AOV_02180] and [AOV_02195 and AOV_02200]) each for infB (AOV 02590), pepA (AOV_03525), thiE (AOV_01015) purA (AOV_03080) and trxB2 (AOV_02200). There were truncated pseudogenes for methylmalonyl-CoA carboxyltransferase (AOV_02035), sucD (AOV_00515), GTPase ObgE (AOV_03085), and dihydrolipoyl dehydrogenase (AOV_02835). There was a pseudogene (AOV_05010) for...”
ANPL_01025 leucyl aminopeptidase from Anaplasma platys
50% identity, 88% coverage
ZMO1309 leucyl aminopeptidase from Zymomonas mobilis subsp. mobilis ZM4
45% identity, 94% coverage
A8GMB0 Probable cytosol aminopeptidase from Rickettsia akari (strain Hartford)
44% identity, 95% coverage
pepA / AAA26388.1 aminopeptidase A from Rickettsia prowazekii (see paper)
P27888 Cytosol aminopeptidase from Rickettsia prowazekii (strain Madrid E)
47% identity, 84% coverage
PSHAa1484 putative cytosol aminopeptidase 1 (EC 3.4.11.1) (Leucine aminopeptidase 1) (LAP 1) (Leucyl aminopeptidase 1) from Pseudoalteromonas haloplanktis TAC125
40% identity, 86% coverage
SO0959 cytosol aminopeptidase from Shewanella oneidensis MR-1
40% identity, 85% coverage
- Transcriptome profiling of Shewanella oneidensis gene expression following exposure to acidic and alkaline pH
Leaphart, Journal of bacteriology 2006 - “...regulator), so0860 (response regulator), pepA-1 (so0959, cytosol aminopeptidase), nhaA (so1336, Na/H antiporter), phoB (so1558, phosphate regulon...”
- “...kinase/response regulator), so0860 (response regulator), so0959 (cytosol aminopeptidase PepA-1), so1336 (Na/H antiporter NhaA), so1558 (phosphate regulon...”
C3K6G5 Probable cytosol aminopeptidase from Pseudomonas fluorescens (strain SBW25)
41% identity, 84% coverage
Avin_11650 Leucyl aminopeptidase from Azotobacter vinelandii AvOP
40% identity, 84% coverage
NE0441 aminopeptidase A/I from Nitrosomonas europaea ATCC 19718
41% identity, 83% coverage
PP0980 cytosol aminopeptidase from Pseudomonas putida KT2440
38% identity, 91% coverage
O86436 bacterial leucyl aminopeptidase (EC 3.4.11.10) from Pseudomonas putida (see paper)
3h8fA / O86436 High ph native structure of leucine aminopeptidase from pseudomonas putida (see paper)
37% identity, 98% coverage
- Ligands: zinc ion; manganese (ii) ion; bicarbonate ion (3h8fA)
O68822 Cytosol aminopeptidase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
B7UVT6 Probable cytosol aminopeptidase from Pseudomonas aeruginosa (strain LESB58)
PA3831 leucyl aminopeptidase from Pseudomonas aeruginosa PAO1
42% identity, 79% coverage
- Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent
Ozdemir, ACS omega 2020 - “...ribosomes and protein Q9HZC5 aminopeptidase N pepN PA3083 peptide catabolic process, proteolysis ribosomes and protein O68822 cytosol aminopeptidase pepA phpA, PA3831 release of an N-terminal amino acid, processing and regular turnover of intracellular proteins. ribosomes and protein Q9HT06 membrane protein insertase YidC yidC PA5568 membrane insertase...”
- Proteomic profiling spotlights the molecular targets and the impact of the natural antivirulent umbelliferone on stress response, virulence factors, and the quorum sensing network of Pseudomonas aeruginosa
Kasthuri, Frontiers in cellular and infection microbiology 2022 - “...1.63 Q88FB2 SuccinateCoA ligase SucC 0.00 1.51 Q8XVS2 Arabinose import ATP-binding protein AraG 0.01 1.96 B7UVT6 Probable cytosol aminopeptidase PepA 0.02 2.71 B7V8B5 UPF0250 protein PLES_09781 0.02 2.31 Q8XWT3 Pantothenate synthetase PanC 0.02 1.79 Q1I2H5 tRNA modification GTPase MnmE 0.02 4.14 P47203 Cell division protein FtsA...”
- Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase
Liao, Computational and structural biotechnology journal 2021 - “...the ability to block the production of P. fluorescens AHL, we first selected PF4660 and PA3831 from the AHL-producing P. fluorescens 2P24 and P. aeruginosa PAO1 respectively. The introduction of plasmid-borne PF4660 or PA3831 into 2P24 could not inhibit AHL production ( Fig. 4 B &...”
- Pseudomonas aeruginosa Presents Multiple Vital Changes in Its Proteome in the Presence of 3-Hydroxyphenylacetic Acid, a Promising Antimicrobial Agent
Ozdemir, ACS omega 2020 - “...N pepN PA3083 peptide catabolic process, proteolysis ribosomes and protein O68822 cytosol aminopeptidase pepA phpA, PA3831 release of an N-terminal amino acid, processing and regular turnover of intracellular proteins. ribosomes and protein Q9HT06 membrane protein insertase YidC yidC PA5568 membrane insertase activity, insertion and/or proper folding...”
- Acyl Carrier Protein 3 Is Involved in Oxidative Stress Response in Pseudomonas aeruginosa
Chen, Frontiers in microbiology 2018 - “...6.91 8.47E-03 PA5483 AlgB Alginate biosynthesis transcriptional regulator 0 6 6.09 0.00393 Acp1 PAO1 CW22 PA3831 PepA Cytosol aminopeptidase 0 49 46 1.70E-16 PA3583 GlpR Glycerol-3-phosphate regulon repressor 0 23 41 1.64E-08 PA2967 FabG 3-Oxo-acyl-ACP reductase 0 8 15.6 8.68E-04 PA4277 Tuf1 Elongation factor Tu 0...”
- Pseudomonas aeruginosa cells attached to a surface display a typical proteome early as 20 minutes of incubation
Crouzet, PloS one 2017 - “...other proteases such as CtpA (PA5134, R = 0.24), Lon (PA1803, R = 0.09), PepA (PA3831, R = 0.26) and PA3787 (R = 0.02) ( S6B Table ). Altogether our data suggested that neither the las system nor the rhl system are necessary for early attachment...”
- A new transcriptional repressor of the pseudomonas aeruginosa quorum sensing receptor gene lasR
Longo, PloS one 2013 - “...PA0779 lon probable ATP-dependent protease 88.6 276-11 709-34 PA3699 probable transcriptional regulator 26.3 - 279-13 PA3831 pepA leucine aminopeptidase 52.6 891-24 412-20 PA4135 probable transcriptional regulator 16.5 293-10 258-9 PA4232 ssb single-stranded DNA-binding protein 18.5 - 134-6 PA4238 rpoA DNA-directed RNA polymerase alpha chain 39.7 -...”
- Bis-(3'-5')-cyclic dimeric GMP regulates antimicrobial peptide resistance in Pseudomonas aeruginosa
Chua, Antimicrobial agents and chemotherapy 2013 - “...PA0427 oprM PA0018 fmt PA1803 PA3135 PA4231 PA3114 PA3831 PA3159 PA5054 PA4225 PA0426 PA1288 PA4749 PA5213 PA3478 PA2086 PA4336 PA4595 PA4476 PA2290 PA5237...”
- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA3576 PA3620 PA3670 PA3702 PA3704 PA3721 PA3733 PA3826 PA3831 PA3837 PA4016 PA4025 PA4109 PA4204 PA4210 PA4223 PA4292 PA4354 PA4355 PA4392 PA4402 PA4406 PA4429...”
- “...PA0090 PA0958 PA1095 PA1348 PA1549 PA2023 PA2693 PA3224 PA3702 PA3831 PA4109 PA4429c PA4431 PA4490 PA4600 PA5038 PA5193 --c --c Gene name Log2 change in the...”
- Genome diversity of Pseudomonas aeruginosa isolates from cystic fibrosis patients and the hospital environment
Finnan, Journal of clinical microbiology 2004 - “...pvdA pilA pilB Bacto1 Pf1 (PA722- (PA0044)b (PA3831) (PA3841) (PA2191) (PA0051) 1905) (PA4209) 4216) (PA4217) (PA1249) (PA1871) (PA3724) (PA2386) (PA4525)...”
Q02RY8 Probable cytosol aminopeptidase from Pseudomonas aeruginosa (strain UCBPP-PA14)
PA14_14470 leucine aminopeptidase from Pseudomonas aeruginosa UCBPP-PA14
42% identity, 79% coverage
- Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase
Simpson, Biochemistry 2023 - “...metal ion dependence, and substrate selectivity of PepA from Pseudomonas aeruginosa ( Pa PepA, UniProt Q02RY8 ). P. aeruginosa is an exceptionally adaptable organism, successfully colonizing soil, water, human skin, and lungs, as well as plants. In a laboratory setting, P. aeruginosa can survive in a...”
- “...Purification of Pa PepA PepA from P. aeruginosa strain PA14 ( Pa PepA, PA14_14470, UniProt: Q02RY8 ) was synthesized as a codon-optimized gBlock (IDT). The Pa PepA gene was inserted into a pJ414 expression plasmid to encode an N-terminal His 6 tag, followed by a linker...”
- Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase
Simpson, Biochemistry 2023 - “...Expression, and Purification of Pa PepA PepA from P. aeruginosa strain PA14 ( Pa PepA, PA14_14470, UniProt: Q02RY8 ) was synthesized as a codon-optimized gBlock (IDT). The Pa PepA gene was inserted into a pJ414 expression plasmid to encode an N-terminal His 6 tag, followed by...”
- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA14_16500 PA14_16470 PA14_16280 PA14_16130 PA14_14520 PA14_14470 PA14_14380 PA14_11900 PA14_11760 PA14_10800 PA14_09550 PA14_09480 PA14_09300 PA14_55770...”
- “...PA14_44440 PA14_38350 PA14_29290 PA14_22480 PA14_16500 PA14_14470 PA14_10800 PA14_57540 PA14_57570 PA14_58260 PA14_60860 PA14_66600 PA14_68610 PA14_18070...”
8pz0A / Q02RY8 Intracellular leucine aminopeptidase of pseudomonas aeruginosa pa14. (see paper)
42% identity, 78% coverage
- Ligand: bicarbonate ion (8pz0A)
wcw_0972 leucyl aminopeptidase from Waddlia chondrophila WSU 86-1044
38% identity, 87% coverage
- Identification of immunogenic proteins of Waddlia chondrophila
Kebbi-Beghdadi, PloS one 2012 - “...20 (Wim20) wcw_0589 GENE ID: 9277568 rplA 50S ribosomal protein L1 70 58 21 (Wim21) wcw_0972 GENE ID: 9277951 pepA Leucyl aminopeptidase No homolog 46 22 (Wim22) wcw_1647 GENE ID: 9278626 rho Transcription termination factor rho 85 79 23 (Wim23) wcw_0999 GENE ID: 9277978 lpdA2 2-oxoglutarate...”
lpg2631 aminopeptidase A/I from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
40% identity, 84% coverage
- Role of Legionella pneumophila outer membrane vesicles in host-pathogen interaction
Ayesha, Frontiers in microbiology 2023 - “...Protein folding, sorting or degradation; stress Francisella novicida ( Pierson et al., 2011 ) Aminopeptidase lpg2631 Protein folding, sorting or degradation Pseudomonas aeruginosa ( Bauman and Kuehn, 2006 ) Peptidylprolyl cis-trans isomerase B (ppiB) lpg2726 Protein folding, sorting, or degradation Pseudomonas aeruginosa ( Choi et al.,...”
- Epidemiological information is key when interpreting whole genome sequence data - lessons learned from a large Legionella pneumophila outbreak in Warstein, Germany, 2013
Petzold, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 2017 - “...NA b YP_096634.1 1 4 13 lpg2630 2971214 2972215 NA b YP_096635.1 1 4 15 lpg2631 2972463 2973947 pepA YP_096636.1 1 4 25 lpg2633 2974501 2974818 NA b YP_096638.1 3 5 5 lpg2635 2976210 2977781 mviN YP_096640.1 1 4 26 lpg2636 2978139 2978405 rpsT YP_096641.1 1...”
GSU0332 aminopeptidase A/I from Geobacter sulfurreducens PCA
39% identity, 87% coverage
- Importance of c-Type cytochromes for U(VI) reduction by Geobacter sulfurreducens
Shelobolina, BMC microbiology 2007 - “...first genetic characterization of one putative periplasmic cytochrome, GSU0616, and two putative outer membrane cytochromes, GSU0332 and GSU1334. Impact of cytochrome knockouts on U(VI) reduction On average, the impact of knocking out confirmed or putative outer membrane cytochromes on the rate of U(VI) reduction was greater...”
- DNA microarray and proteomic analyses of the RpoS regulon in Geobacter sulfurreducens
Núñez, Journal of bacteriology 2006 - “...5 6.1 5.6 404 719 2.1 2.4 19 2 GSU0332 GSU3096 52,228 23,229 6 4.9 1167 2.1 54 GSU0580 97,111 5.6 Dehydrogenase complex E2-component, dihydrolipamide...”
- “...ET AL. proteins (GSU2435, GSU3294, GSU1921, GSU1158, GSU658, GSU0332, GSU3096, and GSU0580) whose abundance was significantly affected by RpoS but for which the...”
CCNA_01028 cytosol aminopeptidase from Caulobacter crescentus NA1000
41% identity, 73% coverage
ABUW_3646 leucyl aminopeptidase from Acinetobacter baumannii
41% identity, 80% coverage
F3B38_RS08900, J3P46_RS08815 leucyl aminopeptidase from Janthinobacterium lividum
34% identity, 93% coverage
- Janthinobacterium sp. Strain SLB01 as Pathogenic Bacteria for Sponge Lubomirskia baikalensis
Chernogor, Pathogens (Basel, Switzerland) 2022 - “...Annotation RpoN J3P46_RS03515 1479 F3B38_RS03495 1479 100 RNA polymerase factor sigma-54 (RpoN) PepA J3P46_RS08815 1494 F3B38_RS08900 1494 100 leucyl aminopeptidase (PepA Zooglea sp.) Over expression could circumvent the requirement of rpoN, prsK and prsR for the floc-forming phenotype by fixing the exopolysaccharides to bacterial cells. XrtA...”
- “...Identity % Annotation RpoN J3P46_RS03515 1479 F3B38_RS03495 1479 100 RNA polymerase factor sigma-54 (RpoN) PepA J3P46_RS08815 1494 F3B38_RS08900 1494 100 leucyl aminopeptidase (PepA Zooglea sp.) Over expression could circumvent the requirement of rpoN, prsK and prsR for the floc-forming phenotype by fixing the exopolysaccharides to bacterial...”
FN1906 Cytosol aminopeptidase from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
41% identity, 73% coverage
- Proteomics of Fusobacterium nucleatum within a model developing oral microbial community
Hendrickson, MicrobiologyOpen 2014 - “...that several proteases also showed increased levels, though those proteins (FN0590, FN0873, FN1186, FN1280, FN1281, FN1906) are spread across a number of different functions. Of the three annotated endopeptidases (FN0266, FN0477, FN1280), one (FN0477) showed decreased levels in all mixed samples, though not to the same...”
- “...FN1128, FN1145, FN1186, FN1205, FN1271, FN1277, FN1280, FN1281, FN1297, FN1322, FN1426, FN1449, FN1728, FN1804, FN1826, FN1906, FN1931, FN1950, FN1978, FN2014, FN2016, FN2100. Protein secretion As seen in Table S11, the detected protein secretion proteins were virtually all decreased in mixed communities compared to Fn alone. The...”
DVU0415 cytosol aminopeptidase from Desulfovibrio vulgaris Hildenborough
38% identity, 85% coverage
APL_1101 putative cytosol aminopeptidase from Actinobacillus pleuropneumoniae L20
37% identity, 90% coverage
- Effects of growth conditions on biofilm formation by Actinobacillus pleuropneumoniae
Labrie, Veterinary research 2010 - “...APL_1056 APL_1056 Transposase 1.560 APL_0985 APL_0985 Transposase 1.271 Protein fate APL_0871 pepE Peptidase E 2.551 APL_1101 pepA Putative cytosol aminopeptidase 1.913 APL_0254 pepD Aminoacyl-histidine dipeptidase 1.903 APL_1883 ptrA Protease 3 precursor 1.680 APL_0928 hscB Co-chaperone protein HscB-like protein 1.377 APL_1068 secF Protein-export membrane protein SecF 1.496...”
Q8ZBH3 Probable cytosol aminopeptidase from Yersinia pestis
YPO3441 cytosol aminopeptidase from Yersinia pestis CO92
y0746 aminopeptidase A/I from Yersinia pestis KIM
38% identity, 82% coverage
- Investigation of Yersinia pestis Laboratory Adaptation through a Combined Genomics and Proteomics Approach
Leiser, PloS one 2015 - “...ilvC/YPO3888 Ketol-acid reductoisomerase Q8ZAC2 2.48/2.38 NI/NI iscS/YPO2896 Cysteine desulfrase Q7CJN7 2.90/NI NI/NI pepA/YPO344 Leucyl aminopeptidase Q8ZBH3 2.92/2.56 2.89/2.90 pepB/YPO2889 Aminopeptidase B P58475 2.20/2.10 6.18/7.19 proV/YPO2647 Glycine betaine/L-proline transport ATP-binding protein Q0WDN9 NI/NI 0.48/NI selD/YPO2164 Selenophosphate synthase Q8ZEK1 AO/AO NI/NI trpE/YPO2208 Anthranilate synthase component Q7CIM3 NI/NI AO/AO...”
- Investigation of Yersinia pestis Laboratory Adaptation through a Combined Genomics and Proteomics Approach
Leiser, PloS one 2015 - “...the same as ancestral cells. PepA mutations result in downstream changes to CarAB expression PepA (YPO3441) encodes a multifunctional leucyl aminopeptidase/DNA binding protein [ 52 ]. Aside from its eponymous function, it is an accessory protein required for proper plasmid resolution partitioning via the Xer system...”
- Recombinant expression and functional analysis of proteases from Streptococcus pneumoniae, Bacillus anthracis, and Yersinia pestis
Kwon, BMC biochemistry 2011 - “...y0125, sigma cross-reacting protein 27A, 4: y0137, protease DO, 5: y0720, putative PhnP protein, 6: y0746, cytosol aminopeptidase, 7: y1280, pyrrolidone-carboxylate peptidase, 8: y2013, putative pepetidase, 9: y2057, peptidase U7 family SohB protein, 10: y2527, prolyl oligopeptidase family protein, 11: y2694, conserved hypothetical protein, 12: y3230,4-methyl-5(b-hydroxyethyl)-thiazole...”
FTN_0660 cytosol aminopeptidase from Francisella tularensis subsp. novicida U112
39% identity, 89% coverage
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...Cytosolic L-cysteinyl glycine (di-peptide) Breakdown (Figure 1A/B) cytosol leucyl Aminopeptidase A, pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B) Glutamate-CSH...”
- “...2020 ) No effect reported ( Ireland etal., 2019 ) pepA **/$ Amino peptidase A FTN_0660; FTT_1318c; FTL_1479. 1.4 2.51 No Change at 4/8h. Essential ( Ramseyetal., 2020 ) (1.36 log2FC) Not essential ( Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase...”
- Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida
Cui, Scientific reports 2018 - “...is conserved among the F. tularensis subspecies. Genome annotation predicts that the five upstream genes (FTN_0660 to FTN_0664) and the downstream uvrA gene are transcribed in the same orientation, suggesting the potential cotranscription of these genes. We tested this possibility by amplifying the RNA transcripts that...”
- Francisella-arthropod vector interaction and its role in patho-adaptation to infect mammals
Akimana, Frontiers in microbiology 2011 - “...protein, pseudogene FTN_0651 Cytidine deaminase cdd FTN_0652 Uridine phosphorylase udp FTN_0653 tRNA-(ms(2)io(6)a)-hydroxylase miaE FTN_0655 Methylase FTN_0660 Cytosol aminopeptidase pepA FTN_0664 Type IV pili fiber building block protein FTN_0666 Excinuclease ABC, subunit A uvrA FTN_0667 Major facilitator superfamily (MFS) transport protein yieO FTN_0672 Preprotein translocase, subunit A...”
FTT_1318c cytosol aminopeptidase from Francisella tularensis subsp. tularensis SCHU S4
FTL_1479 cytosol aminopeptidase from Francisella tularensis subsp. holarctica
39% identity, 89% coverage
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...FTL_1495/FTL_1496# Cytosolic L-cysteinyl glycine (di-peptide) Breakdown (Figure 1A/B) cytosol leucyl Aminopeptidase A, pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B)...”
- “...) No effect reported ( Ireland etal., 2019 ) pepA **/$ Amino peptidase A FTN_0660; FTT_1318c; FTL_1479. 1.4 2.51 No Change at 4/8h. Essential ( Ramseyetal., 2020 ) (1.36 log2FC) Not essential ( Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase B...”
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...L-cysteinyl glycine (di-peptide) Breakdown (Figure 1A/B) cytosol leucyl Aminopeptidase A, pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B) Glutamate-CSH ligase,...”
- “...No effect reported ( Ireland etal., 2019 ) pepA **/$ Amino peptidase A FTN_0660; FTT_1318c; FTL_1479. 1.4 2.51 No Change at 4/8h. Essential ( Ramseyetal., 2020 ) (1.36 log2FC) Not essential ( Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase B FTN_0780;...”
- Glutathione provides a source of cysteine essential for intracellular multiplication of Francisella tularensis
Alkhuder, PLoS pathogens 2009 - “...cysteinylglycine [39] . We identified the three orthologs of these aminopeptidases in the LVS genome (FTL_1479, FTL_1956, and FTL_1108, sharing 48%, 48% and 38% amino acid identity with PepA, B and N, respectively). It is thus reasonable to assume that LVS has the capacity to efficiently...”
CPn0385 Leucyl Aminopeptidase A from Chlamydophila pneumoniae CWL029
37% identity, 89% coverage
HI1705 aminopeptidase A/I (pepA) from Haemophilus influenzae Rd KW20
P45334 Cytosol aminopeptidase from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
35% identity, 98% coverage
- Effect of multiple mutations in the hemoglobin- and hemoglobin-haptoglobin-binding proteins, HgpA, HgpB, and HgpC, of Haemophilus influenzae type b
Morton, Infection and immunity 1999 - “...of California, Berkeley Strains H. influenzae HI689 HI1704 HI1705 HI1706 HI1707 HI1708 HI1709 HI1710 HI1711 HI1712 HI1713 HI1714 HI1715 HI1716 HI1717 Reference...”
- “...the previously described hgpA single mutant (HI1705, previously designated HI689hgpADBglII), the hgpB single mutant (HI1706, previously designated...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...multocida PepB (NUMG); H.iPepB, H. influenzae PepB (P45334); S.tiPepB, serovar Typhi PepB (SSC); S.tmPepB, serovar Typhimurium PepB (AF201078 [this work]);...”
- Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa
Woolwine, Journal of bacteriology 1999 - “...from the SwissProt database (accession no. P11648, P45334, P27288, and Q10401, respectively). Additional sequence information was obtained from the Pseudomonas...”
WQ49_RS26825 leucyl aminopeptidase from Burkholderia cenocepacia
37% identity, 89% coverage
Hsero_3109 leucyl aminopeptidase from Herbaspirillum seropedicae SmR1
37% identity, 90% coverage
CAB397 leucyl aminopeptidase from Chlamydia abortus S26/3
37% identity, 88% coverage
CD1300 leucyl aminopeptidase from Clostridium difficile 630
33% identity, 98% coverage
HBA75_24805 cytosol aminopeptidase from Bacillus thuringiensis
38% identity, 85% coverage
BAS4792 cytosol aminopeptidase from Bacillus anthracis str. Sterne
C3LC57 Probable cytosol aminopeptidase from Bacillus anthracis (strain CDC 684 / NRRL 3495)
Q81XS5 Probable cytosol aminopeptidase from Bacillus anthracis
BA5155 cytosol aminopeptidase from Bacillus anthracis str. Ames
38% identity, 85% coverage
- Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis
Arora, NPJ biofilms and microbiomes 2017 - “...factor-Ts PrkC-influenced 1 Bas4213, dnaK Molecular chaperone DnaK 2 Bas0253, groEL GroEL, 60kDa chaperone 3 Bas4792, pepA Leucyl aminopeptidase 4 Bas0330 Alkyl hydroperoxide reductase 5 Bas0295 1-Pyrroline-5-carboxylate dehydrogenase 6 Bas3392 DNA topoisomerase IV subunit B, DNA gyrase 7, 8 Bas0108, tuf Elongation factor-Tu 9 Bas4297, aspS...”
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...as B. cereus ATCC 14579 (Q816E3), B. thuringiensis subsp. tolworthi (A0A0G4CV43), B. anthracis CDC 684 (C3LC57), B. mycoides (A0A1X6QF78), B. weihenstephanensis FSL H7-687 (W4E7C7), B. pseudomycoides DSM 12442 (C3BRS1) and B. cytotoxicus DSM 22905 (A7GUC8), but lower identity with other species, e.g., 38% identity with LAP...”
- “...insert found when the C-terminal domains of bcLAP were compared with those of C3BRS1, A7GUC8, C3LC57, A0A0G4CV43 and W4E7C7. The aforementioned M17 peptidases possessed the highly conserved primary structure of the C-terminal domain, which allowed us to predict the active residues and those involved in the...”
- Genomics and Proteomics Analyses Revealed Novel Candidate Pesticidal Proteins in a Lepidopteran-Toxic Bacillus thuringiensis Strain
Khorramnejad, Toxins 2020 - “...70 65 Glucose-6-phosphate isomerase Q816G0 16 41 Other functions DNA protection Q8RPQ1 45 67 Aminopeptidase Q81XS5 37 37 Superoxide dismutase Q81LW0 38 90 Pyridoxal 5-phosphate synthase A9VM99 31 55 Cytosol aminopeptidase Q816E3 32 37 Purine nucleoside phosphorylase Q81T09 8 21 Major cold shock protein Q81TW8 2...”
- Formation and composition of the Bacillus anthracis endospore
Liu, Journal of bacteriology 2004 - “...4.9 5 5 4.8 5 5.4 5.2 5.1 5 5.9 5 BA4815 BA4861 BA4933 BA4947 BA5155 BA5380 9 7 8 10 3 10 39 22 16 29 7 42 24.1 29.6 23.5 45.7 7.5 51.8 361 365 371 357 494 193...”
Q816E3 Probable cytosol aminopeptidase from Bacillus cereus (strain ATCC 14579 / DSM 31 / CCUG 7414 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NCTC 2599 / NRRL B-3711)
38% identity, 85% coverage
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...identity with cytosolic aminopeptidases from the Bacillus cereus group, such as B. cereus ATCC 14579 (Q816E3), B. thuringiensis subsp. tolworthi (A0A0G4CV43), B. anthracis CDC 684 (C3LC57), B. mycoides (A0A1X6QF78), B. weihenstephanensis FSL H7-687 (W4E7C7), B. pseudomycoides DSM 12442 (C3BRS1) and B. cytotoxicus DSM 22905 (A7GUC8), but...”
- Genomics and Proteomics Analyses Revealed Novel Candidate Pesticidal Proteins in a Lepidopteran-Toxic Bacillus thuringiensis Strain
Khorramnejad, Toxins 2020 - “...37 37 Superoxide dismutase Q81LW0 38 90 Pyridoxal 5-phosphate synthase A9VM99 31 55 Cytosol aminopeptidase Q816E3 32 37 Purine nucleoside phosphorylase Q81T09 8 21 Major cold shock protein Q81TW8 2 22 1 The number of distinct matching peptides having at least a 95% level of confidence....”
CT045 Leucyl Aminopeptidase A from Chlamydia trachomatis D/UW-3/CX
36% identity, 88% coverage
- Chlamydia trachomatis Alters Mitochondrial Protein Composition and Secretes Effector Proteins That Target Mitochondria
Dimond, mSphere 2022 - “...rpmE2 2 24 0.047 CTL0283 (CT028) 50S ribosomal protein L19, rplS 3 26 0.146 CTL0301 (CT045) Leucyl aminopeptidase, pepA 4 8 0.015 CTL0321 (CT065) ADP, ATP carrier protein, ntp1 3 4 0.229 CTL0322 (CT066) Hypothetical protein 2 23 0.342 CTL0323 (CT067) ABC transporter substrate-binding protein, ytgA...”
- Outer membrane proteins preferentially load MHC class II peptides: implications for a Chlamydia trachomatis T cell vaccine
Karunakaran, Vaccine 2015 - “...live C. trachomatis for 12 h. Peptide Chlamydia trachomatis locus# Source proteins Protein abbrev. YKLVYQNALSNFSGKK CT045 Leucyl aminopeptidase PepA GPKGRHVVIDKSFGSPQVTKDGVT CT110 Chaperonin GroEL1 41 Hsp60 EERVVGQPFAIAAVSDS CT113 Clp Protease ATPase ClpB DLKVTGPTIHTDLD CT143 Hypothetical protein 33 CT143 GKLIVTNPKSDISFGG CT144 Hypothetical protein 60 CT144 GSPGQTNYAAAKAGIIGFS CT237 3-Ketoacyl-(acyl-carrier-protein)...”
- Autoprocessing and self-activation of the secreted protease CPAF in Chlamydia-infected cells
Chen, Microbial pathogenesis 2010 - “...used: For cloning CT020, 5-CGC GGATCC .ATG.ACG.AGC.AGT.TAC.ATG.AGT-3(forward) and 5-TTTCCTTTT GCGGCCGC .TTA.AGG.GAA.TAG.CCT.ATG.GTT -3 (reverse); For cloning CT045, 5-CGC GGATCC .ATG.AAG.AAT.TCT.AAA.GCT.CAA.GAA-3 (forward) and 5-TTTCCTTTT GCGGCCGC .CTA.TTTAGATAGGAATTTCTCCAT-3 (reverse); For cloning CT072, 5-CCG GAATTC .ATG.ACA.ATA.ATA.TAT.TTT.GTT.CTT-3 (forward) and 5-TTTCCTTTT GCGGCCGC .TCA.CCC.AAC.GAA.AAC.TCT.AGA-3(reverse); For cloning CT082, 5-CGC GGATCC .ATG.TCA.ATT.TCT.GGA.AGT.GGT-3(forward) and 5-TTTCCTTTT GCGGCCGC .TCA.TGA.ATC.GCC.TCC.TGC-3(reverse);...”
- The ompA gene in Chlamydia trachomatis differs in phylogeny and rate of evolution from other regions of the genome
Brunelle, Infection and immunity 2006 - “...araD adk hemZ gap thdF uvrC mrsA CT044 CT045 CT121 CT128 CT485 CT505 CT698 CT791 CT815 Noncoding regions ssb-pepA rpoB-r17 rs4-yceA thdF-psdD glyQ-pgsA porB...”
6omeA / O84049 Crystal structure of a probable cytosol aminopeptidase (leucine aminopeptidase, lap) from chlamydia trachomatis d/uw-3/cx
36% identity, 88% coverage
- Ligands: carbonate ion; zinc ion (6omeA)
W8YIL1 Probable cytosol aminopeptidase from Bacillus thuringiensis DB27
38% identity, 85% coverage
Q6Q833 leucyl aminopeptidase (EC 3.4.11.1) from Trypanosoma brucei brucei (see paper)
49% identity, 42% coverage
Q57W67 leucyl aminopeptidase (EC 3.4.11.1) from Trypanosoma brucei brucei (see paper)
49% identity, 42% coverage
CTL0301 putative aminopeptidase from Chlamydia trachomatis 434/Bu
B0B9F3 Probable cytosol aminopeptidase from Chlamydia trachomatis serovar L2 (strain ATCC VR-902B / DSM 19102 / 434/Bu)
36% identity, 88% coverage
- Requirement of GrgA for Chlamydia infectious progeny production, optimal growth, and efficient plasmid maintenance
Lu, mBio 2024 - “...stress responses in Chlamydia . Similarly, four protease genes ( lon, htrA, ftsH , and ctl0301 ), four protein chaperone genes ( clpB, clpC, groEL , and groES ), and two enzymes that regulate disulfide bonding in proteins ( ctl0152 and trxB ) also displayed increased...”
- Chlamydia trachomatis Alters Mitochondrial Protein Composition and Secretes Effector Proteins That Target Mitochondria
Dimond, mSphere 2022 - “...L31, rpmE2 2 24 0.047 CTL0283 (CT028) 50S ribosomal protein L19, rplS 3 26 0.146 CTL0301 (CT045) Leucyl aminopeptidase, pepA 4 8 0.015 CTL0321 (CT065) ADP, ATP carrier protein, ntp1 3 4 0.229 CTL0322 (CT066) Hypothetical protein 2 23 0.342 CTL0323 (CT067) ABC transporter substrate-binding protein,...”
- Genomewide Transcriptional Responses of Iron-Starved Chlamydia trachomatis Reveal Prioritization of Metabolic Precursor Synthesis over Protein Translation
Brinkworth, mSystems 2018 - “...CTL0549 1.89 0.01 RNase III Transcriptional regulation B0B7L3 CTL0823 CTL0823 1.88 7.21E4 Hypothetical Hypothetical A0A0H3MLF6 CTL0301 CTL0301 1.81 1.32E4 Probable cytosol aminopeptidase PepA Protein processing and folding B0B9F3 CTL0884 CTL0884 1.81 7.04E3 Hypothetical Hypothetical A0A0H3MCL0 CTL0885 CTL0885 1.77 3.48E3 Hypothetical effector Type III secretion A0A0H3MHM9 lpdA...”
- Genomewide Transcriptional Responses of Iron-Starved Chlamydia trachomatis Reveal Prioritization of Metabolic Precursor Synthesis over Protein Translation
Brinkworth, mSystems 2018 - “...Hypothetical Hypothetical A0A0H3MLF6 CTL0301 CTL0301 1.81 1.32E4 Probable cytosol aminopeptidase PepA Protein processing and folding B0B9F3 CTL0884 CTL0884 1.81 7.04E3 Hypothetical Hypothetical A0A0H3MCL0 CTL0885 CTL0885 1.77 3.48E3 Hypothetical effector Type III secretion A0A0H3MHM9 lpdA CTL0820 1.76 3.80E3 Dihydrolipoyl dehydrogenase Energy metabolism A0A0H3MHJ2 CTL0096 CTL0096 1.72 1.98E4...”
A7GUC8 Probable cytosol aminopeptidase from Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98)
36% identity, 85% coverage
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...B. weihenstephanensis FSL H7-687 (W4E7C7), B. pseudomycoides DSM 12442 (C3BRS1) and B. cytotoxicus DSM 22905 (A7GUC8), but lower identity with other species, e.g., 38% identity with LAP from the fungus Coprinus cinereus (Q8TGE4), 35% identity with LAP from Mucor ambiguus (A0A0C9MT45), 33% identity with the 487...”
- “...no insert found when the C-terminal domains of bcLAP were compared with those of C3BRS1, A7GUC8, C3LC57, A0A0G4CV43 and W4E7C7. The aforementioned M17 peptidases possessed the highly conserved primary structure of the C-terminal domain, which allowed us to predict the active residues and those involved in...”
SEET0819_06255, STMMW_44231 leucyl aminopeptidase from Salmonella enterica subsp. enterica serovar Tennessee str.
STM4477 aminopeptidase A from Salmonella typhimurium LT2
SEN4230 cytosol aminopeptidase from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
37% identity, 82% coverage
PepA / b4260 aminopeptidase A/I (EC 3.4.11.9; EC 3.4.11.1; EC 3.4.13.18) from Escherichia coli K-12 substr. MG1655 (see 5 papers)
PepA / P68767 aminopeptidase A/I (EC 3.4.11.9; EC 3.4.13.18) from Escherichia coli (strain K12) (see paper)
AMPA_ECOLI / P68767 Cytosol aminopeptidase; Aminopeptidase A/I; Leucine aminopeptidase; LAP; Leucyl aminopeptidase; EC 3.4.11.1; EC 3.4.11.10 from Escherichia coli (strain K12) (see 2 papers)
P68767 leucyl aminopeptidase (EC 3.4.11.1) from Escherichia coli (see paper)
1gytA / P68767 E. Coli aminopeptidase a (pepa) (see paper)
c5360 Cytosol aminopeptidase from Escherichia coli CFT073
NP_418681 aminopeptidase A/I from Escherichia coli str. K-12 substr. MG1655
P68766 Cytosol aminopeptidase from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
b4260 leucyl aminopeptidase from Escherichia coli str. K-12 substr. MG1655
Z5872 aminopeptidase A/I from Escherichia coli O157:H7 EDL933
38% identity, 78% coverage
- function: Probably involved in the processing and regular turnover of intracellular proteins (PubMed:20067529). Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Required for plasmid ColE1 site-specific recombination but not in its aminopeptidase activity. Could act as a structural component of the putative nucleoprotein complex in which the Xer recombination reaction takes place (PubMed:8057849).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
cofactor: Mn(2+) (Binds 2 manganese ions per subunit.)
subunit: Homohexamer
disruption phenotype: A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529). - Ligands: zinc ion; carbonate ion (1gytA)
- The Promoter of the Immune-Modulating Gene TIR-Containing Protein C of the Uropathogenic Escherichia coli Strain CFT073 Reacts to the Pathogen's Environment
Hemberger, International journal of molecular sciences 2022 - “...the macrophage-sensing, two-component system PhoQ/P CFT073 hns hns c1701 DNA-binding protein H-NS CFT073 pepA pepA c5360 Cytosol aminopeptidase CFT073 c4494 c4494 c4494 Putative transcriptional regulator ijms-23-01148-t003_Table 3 Table 3 Primer names and sequences. Upper case letters represent overhangs. Designation Sequence phoQ a 5-catttaaccggaaaaaag-3 phoQ b 5-GCAGCTCCAGCCTACACttatcactacatcaaggc-3...”
- The trigger enzyme PepA (aminopeptidase A) of Escherichia coli, a transcriptional repressor that generates positive supercoiling.
Nguyen, FEBS letters 2016 (PubMed)- GeneRIF: in vitro topology studies performed with DNA minicircles demonstrate that PepA binding constrains a single positive supercoil in the carP1 control region. Such a topological event is understood to constitute an impediment to transcription initiation and may serve as a mechanism to regulate gene expression.
- Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
Kazakov, Journal of bacteriology 2008 - GeneRIF: Data show that PepA can effectively process heptapeptide-nucleotide microcin C (McC).
- Mutagenesis of PepA suggests a new model for the Xer/cer synaptic complex.
Reijns, Molecular microbiology 2005 (PubMed)- GeneRIF: Random PepA mutants unable to support efficient Xer recombination are reported.
- xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase.
Stirling, The EMBO journal 1989 - GeneRIF: N-terminus verified by Edman degradation on complete protein
- The Promoter of the Immune-Modulating Gene TIR-Containing Protein C of the Uropathogenic Escherichia coli Strain CFT073 Reacts to the Pathogen's Environment
Hemberger, International journal of molecular sciences 2022 - “...l a m i n o p e p- t i d a s e P68766 199.08 121.75 16 8 7 3 7 3 54,880 54,880 DNA-binding protein H -N S P0ACF9 177.18 117.92 31 22 6 2 6 2 15,540 15,540 5-methyl-tetrahydropteroyltriglutamate-homocysteine methyltransferase Q8FBM1 156.12...”
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...(Q9CPY7), 37% identity with mosquito LAP (W5J5J1) and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37% identity with Nicotiana tabacum LAP (U3PLK3). However, when the C-terminal domains of these different aminopeptidases, which contain their respective active sites,...”
- “...insertions, one 4-residue insertion, two 1-residue deletions and one 2-residue deletion between bcLAP and ecLAP (P68767), there were five 1-residue deletions and one 1-residue insertion between bcLAP and fungal LAP (A0A0C9MT45), there were two 2-residue deletions, three 1-residue deletions, two 1-residue insertions and one 5-residue insert...”
- An unexpected role for leucyl aminopeptidase in UV tolerance revealed by a genome-wide fitness assessment in a model cyanobacterium
Weiss, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...mutations in Synpcc7942_1190 , a homolog of the Escherichia coli pepA gene (UniProt accession no. P68767), called lap in Synechocystis sp. PCC 6803 ( 16 ), which encodes a putative leucyl aminopeptidase. We assessed the phenotype of a knockout mutant of S. elongatus Synpcc7942_1190 , hereafter...”
- Nucleoid-associated proteins shape chromatin structure and transcriptional regulation across the bacterial kingdom.
Amemiya, Transcription 2021 (no snippet) - Genome-scale identification and characterization of moonlighting proteins
Khan, Biology direct 2014 - “...interacting proteins was observed for E. coli MP. This peak consists of two proteins, pepA (P68767) and frdB (P0AC47). Figure 5 Interacting proteins of moonlighting and non-moonlighting proteins. Physically interacting proteins were obtained from the STRING database. (A) Histogram of the number of interacting proteins. Five...”
- Letters
, The Ulster medical journal 2016 - “...G Tarpley J How long does it take to train a surgeon? BMJ 2009 339 b4260 19892790 2 Maxwell AJ Crocker M Jones TL Bhagawati B Papadopoulos MC Bell BA Implementation of the European Working Time Directive in neurosurgery reduces continuity of care and training opportunities...”
- Metabolic shift of Escherichia coli under salt stress in the presence of glycine betaine
Metris, Applied and environmental microbiology 2014 - “...kinase Conserved hypothetical protein Putative selenate reductase b1241 b1241 b4260 b3556 b2414 b0031 b2095 b2095 b0809 b3213 b2579 b1237 b1237 b3671 2 5 2 2...”
- Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism
Sharma, BMC microbiology 2017 - “...Peptidyl-tRNA hydrolase domain-containing protein 1.36 0.03 rpmE Z5484 50S ribosomal protein L31 2.20 0.019 pepA Z5872 Leucyl aminopeptidase 1.58 0.02 DNA/RNA metabolism hisT Z3580 tRNA pseudouridine synthase A 1.51 0.02 deoD Z5986 Purine nucleoside phosphorylase 1.37 0.04 mazG Z4096 Nucleoside triphosphate pyrophospho-hydrolase +1.49 0.02 Cell wall/cell...”
P0C6E1 leucyl aminopeptidase (EC 3.4.11.1) from Vibrio cholerae (see paper)
45% identity, 58% coverage
LAP / BAB87833.1 leucine aminopeptidase from Coprinopsis cinerea (see paper)
Q8TGE4 Leucine aminopeptidase from Coprinopsis cinerea
40% identity, 67% coverage
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ.
Liu, International journal of molecular sciences 2023 - “...lower identity with other species, e.g., 38% identity with LAP from the fungus Coprinus cinereus (Q8TGE4), 35% identity with LAP from Mucor ambiguus (A0A0C9MT45), 33% identity with the 487 amino acid sequence of B. taurus LAP (P00727), 32% identity with human leucyl-aminopeptidase (P28838), 36% identity with...”
- “...leucyl-aminopeptidases from Escherichia coli (P68767), Arabidopsis thaliana (Q944P7), Nicotiana tabacum (U3PLK3 ) , C. cinereus (Q8TGE4), Mucor ambiguous ( A0A0C9MT45), mosquito (W5J5J1 ) , mouse (Q9CPY7), human (P28838) and B. taurus (P00727). Asterisk (*): identical amino acid residues in all proteins. Solid boxes: putative catalytic residues....”
XF0138 aminopeptidase A/I from Xylella fastidiosa 9a5c
Q9PH08 Probable cytosol aminopeptidase from Xylella fastidiosa (strain 9a5c)
XF_0138 leucyl aminopeptidase from Xylella fastidiosa 9a5c
36% identity, 90% coverage
- In vitro Determination of Extracellular Proteins from Xylella fastidiosa
Mendes, Frontiers in microbiology 2016 - “...(Virj family protein) 49 XF2679 XF2329 - Yes No 0.171 Xanthomonadales conserved hypothetical pal 51.7 XF0138 XF0106 Q9PH08 No No 0.095 Xanthomonadales conserved hypothetical 17.9 XF0964 XF0796 - No No 0.359 Xanthomonadales conserved hypothetical 42.3 XF0357 XF0278 - No No 0.898 Xanthomonadales conserved hypothetical 42.6 XF0358...”
- Analysis of the biofilm proteome of Xylella fastidiosa
Silva, Proteome science 2011 - “...9a5c] 57835 5.45 61 III.C.2 XF0615 chaperonin GroEL [Xylella fastidiosa 9a5c] 57835 5.45 133 III.C.2 XF0138 leucyl aminopeptidase [Xylella fastidiosa 9a5c] 52147 6.07 77 III.C.3 XF0381 ATP-dependent Clp protease subunit [Xylella fastidiosa 9a5c] 95805 5.36 211 III.C.3 XF0256 glucose-1-phosphate thymidylyltransferase [Xylella fastidiosa 9a5c] 32757 5.70 164...”
- “...sources or to facilitate the migration of the bacteria within xylem vessels [ 30 ]. XF0138 belongs to a cytosol aminopeptidase family and plays a key role in protein degradation and in the metabolism of biologically active peptides. The protein XF0576 is a predicted metalloendopeptidase and...”
- In vitro Determination of Extracellular Proteins from Xylella fastidiosa
Mendes, Frontiers in microbiology 2016 - “...protein) 49 XF2679 XF2329 - Yes No 0.171 Xanthomonadales conserved hypothetical pal 51.7 XF0138 XF0106 Q9PH08 No No 0.095 Xanthomonadales conserved hypothetical 17.9 XF0964 XF0796 - No No 0.359 Xanthomonadales conserved hypothetical 42.3 XF0357 XF0278 - No No 0.898 Xanthomonadales conserved hypothetical 42.6 XF0358 XF0279 -...”
- Transcriptome and Secretome Analyses of Endophyte Methylobacterium mesophilicum and Pathogen Xylella fastidiosa Interacting Show Nutrient Competition
Dourado, Microorganisms 2023 - “...FkpA 32 7 0.74 51 - XF9a_00181 XF_0196 DUF2059 20 20 26|27 52 - XF9a_00120 XF_0138 Aminopeptidase PepA 52 12 53 - XF9a_00760 XF_0838 Chaperone SurA 51 5 25|26 54 32 XF9a_00744 XF_0820 Zn-dependent amino- or carboxypeptidase 58 9 0.52 55 - XF9a_01399 XF_1527 Type II...”
AMPL_ENCCU / Q8SQZ7 Cytosol aminopeptidase; Leucine aminopeptidase; LAP; Leucyl aminopeptidase; Proline aminopeptidase; Prolyl aminopeptidase; EC 3.4.11.1; EC 3.4.11.5 from Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite) (see paper)
40% identity, 73% coverage
- function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides (By similarity).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Zn(2+) (Binds 2 Zn(2+) ions per subunit.)
subunit: Homohexamer.
NMY220_1465 leucyl aminopeptidase from Neisseria meningitidis NM220
41% identity, 73% coverage
Q5H4N2 leucyl aminopeptidase (EC 3.4.11.1) from Xanthomonas oryzae (see paper)
3jruB / Q5H4N2 Crystal structure of leucyl aminopeptidase (pepa) from xoo0834, xanthomonas oryzae pv. Oryzae kacc10331
XOO0834 aminopeptidase A/I from Xanthomonas oryzae pv. oryzae KACC10331
WP_011257713 leucyl aminopeptidase from Xanthomonas oryzae pv. oryzae
45% identity, 64% coverage
- Ligands: carbonate ion; zinc ion (3jruB)
- Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae
Huynh, Acta crystallographica. Section F, Structural biology and crystallization communications 2009 - “...causes the serious disease bacterial blight in rice. The pepA (Xoo0834) gene from Xoo is one of around 100 genes that have been selected for the design of...”
- “...for the rational development of drugs, the pepA (Xoo0834) gene, which encodes leucine aminopeptidase, was cloned and expressed in Escherichia coli. The...”
- Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of leucine aminopeptidase (LAP) from the pepA gene of Xanthomonas oryzae pv. oryzae.
Huynh, Acta crystallographica. Section F, Structural biology and crystallization communications 2009 - GeneRIF: The LAP crystal diffracted to 2.6 A resolution and belonged to the cubic space group P2(1)3.
O32106 leucyl aminopeptidase (EC 3.4.11.1); bacterial leucyl aminopeptidase (EC 3.4.11.10) from Bacillus subtilis (see paper)
35% identity, 86% coverage
8d1xD / B4RJ22 Crystal structure of aminopeptidase a from neisseria gonorrhoeae
40% identity, 73% coverage
- Ligand: manganese (ii) ion (8d1xD)
NGO1227 AmpA from Neisseria gonorrhoeae FA 1090
40% identity, 73% coverage
XAC3556 aminopeptidase A/I from Xanthomonas axonopodis pv. citri str. 306
44% identity, 64% coverage
C5NSV0 leucyl aminopeptidase (EC 3.4.11.1) from Babesia gibsoni (see paper)
41% identity, 68% coverage
TDE0300 cytosol aminopeptidase family protein from Treponema denticola ATCC 35405
38% identity, 84% coverage
7y1sA Crystal structure of apo leucyl aminopeptidase from bacillus amyloliquefaciens
34% identity, 87% coverage
- Ligands: carbonate ion; zinc ion (7y1sA)
D6WAL0 Cytosol aminopeptidase from Tribolium castaneum
44% identity, 68% coverage
Q7KF27 leucyl aminopeptidase (EC 3.4.11.1) from Leishmania major (see paper)
XP_001683430 cytosolic leucyl aminopeptidase from Leishmania major strain Friedlin
45% identity, 49% coverage
cg2419 leucyl aminopeptidase from Corynebacterium glutamicum ATCC 13032
36% identity, 83% coverage
Q5XGB9 Cytosol aminopeptidase from Xenopus tropicalis
42% identity, 62% coverage
alr0237 leucine aminopeptidase from Nostoc sp. PCC 7120
37% identity, 94% coverage
- Proteomic Insights into Starvation of Nitrogen-Replete Cells of Nostoc sp. PCC 7120 under β-N-Methylamino-L-Alanine (BMAA) Treatment
Koksharova, Toxins 2020 - “...3.7 0.0417 33 Glutathione reductase (NADPH) [EC:1.8.1.7] all4968 Glutathione metabolism 1.49 0.0202 34 leucylaminopeptidase [EC:3.4.11.1] alr0237 Glutathione metabolism 1.49 0.0469 35 aldo/ketoreductase all2316 Positive role of AKR in detoxification of reactive carbonyl species (RCS) produced under oxidative stress 2.04 0.0143 36 polyribonucleotidenucleotidyltransferase [EC:2.7.7.8] all4396 RNA degradation...”
- The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box
Oliveira, Life (Basel, Switzerland) 2015 - “...+ Transport and binding proteins - Alr0169 Cyclomaltodextrin glucanotransferase NH 4 + Other categories - Alr0237 Probable cytosol aminopeptidase N 2 , NH 4 + Translation - Alr0267 Alr0267 protein N 2 , NO 3 No similarity N. punctiforme , Anabaena Alr0474 Alr0474 protein NO 3...”
- “...of proteins and peptides, as well as processes regarding amino acid biosynthesis and processing (All2533, Alr0237, Alr0880, Alr0996, Alr1381, All4287, Alr1742, Alr4853, All2315, Alr1004, Alr1313, Alr2771, Alr0608, Alr1080, Alr2328 Alr4907, All1683, All4464), sugar breakdown and processing (Alr3608, Alr4448, All3964, All0167, All0168, All0875, Alr0169, Alr2190, All4539) and...”
RHE_RS01080 leucyl aminopeptidase from Rhizobium etli CFN 42
38% identity, 76% coverage
- Rhizobium etli CFN42 proteomes showed isoenzymes in free-living and symbiosis with a different transcriptional regulation inferred from a transcriptional regulatory network
Taboada-Castro, Frontiers in microbiology 2022 - “...[EC:2.4.2.7] K05349 MM RHE_RS28885 bglX; beta-glucosidase [EC:3.2.1.21] K05349 Bacteroid RHE_RS29645 bglX; beta-glucosidase [EC:3.2.1.21] K01255 MM RHE_RS01080 CARP, pepA; leucyl aminopeptidase [EC:3.4.11.1] K01255 Bacteroid RHE_RS07430 CARP, pepA; leucyl aminopeptidase [EC:3.4.11.1] K00405 MM RHE_RS29065 ccoO; cytochrome c oxidase cbb3-type subunit II K00405 Bacteroid RHE_RS30885 ccoO; cytochrome c oxidase...”
- “...pathway 3, and Supplementary Table 3A ), for pepA , leucyl aminopeptidase [EC:3.4.11.1] enzyme, the RHE_RS01080 was expressed in MM, while in bacteroid the RHE_RS07430 was identified ( Table 1 ). For the gst , glutathione S-transferase [EC:2.5.1.18] in MM RHE_RS01425, RHE_RS05865, RHE_RS06130, RHE_RS06230, and RHE_RS11855...”
ZMO0806 Leucyl aminopeptidase from Zymomonas mobilis subsp. mobilis ZM4
38% identity, 76% coverage
- Investigation of the impact of a broad range of temperatures on the physiological and transcriptional profiles of Zymomonas mobilis ZM4 for high-temperature-tolerant recombinant strain development
Li, Biotechnology for biofuels 2021 - “...genes ( ZMO0957 , ZMO1571 , ZMO1258 , ZMO1255 , ZMO1253 , ZMO1572 , and ZMO0806 ), glucose metabolism-related genes ( ZMO1757 , ZMO1756 , ZMO1649 , ZMO0366 , ZMO1981 , and ZMO0689 ), glutamate, histidine, and cysteine synthesis-related genes ( ZMO1117 , ZMO0457 , ZMO1964...”
Q0SHL0 Probable cytosol aminopeptidase from Rhodococcus jostii (strain RHA1)
36% identity, 86% coverage
- Metaproteomics reveals major microbial players and their biodegradation functions in a large-scale aerobic composting plant
Liu, Microbial biotechnology 2015 - “...Saccharomycetes Probable M18 family aminopeptidase 2 A4VJG1 Protein degradation Pseudomonas stutzeri Gammaproteobacteria Probable cytosol aminopeptidase Q0SHL0 Protein degradation Rhodococcus jostii Actinobacteria ATP-dependent zinc metalloprotease FtsH Q03Z46 Protein degradation Leuconostoc mesenteroides Bacilli; Lactobacillales ATP-dependent Clp protease ATP-binding subunit ClpX B2JGL6 Protein degradation Burkholderia phymatum Betaproteobacteria ATP-dependent protease...”
LAP3 / Q68FS4 cytosolic leucyl aminopeptidase subunit (EC 3.4.13.23; EC 3.4.13.18; EC 3.4.11.9) from Rattus norvegicus (see 5 papers)
AMPL_RAT / Q68FS4 Cytosol aminopeptidase; Cysteinylglycine-S-conjugate dipeptidase; Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; LAP; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; EC 3.4.11.1; EC 3.4.13.23; EC 3.4.11.5 from Rattus norvegicus (Rat) (see 2 papers)
Q68FS4 cysteinylglycine-S-conjugate dipeptidase (EC 3.4.13.23) from Rattus norvegicus (see 2 papers)
NP_001011910 cytosol aminopeptidase from Rattus norvegicus
41% identity, 60% coverage
- function: Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides (PubMed:12675513, PubMed:6108111). The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme (By similarity). For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates (By similarity). Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status (PubMed:12675513).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine (RHEA:60444)
catalytic activity: L-cysteinylglycine + H2O = L-cysteine + glycine (RHEA:28783)
catalytic activity: S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine (RHEA:62568)
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Zn(2+) Mn(2+) (Binds two metal ions per subunit. Two metal binding sites with different affinities are located in the enzyme active site and can be occupied in vitro by different metals: site 1 is occupied by Zn(2+), Mn(2+), Mg(2+) or Co(2+), while the tight binding site 2 can be occupied by only Zn(2+) or Co(2+). One Zn(2+) ion is tightly bound to site 2 and essential for enzyme activity in vivo, while site 1 can be occupied by different metals to give different enzymatic activities. Mn(2+) is required for Cys-Gly hydrolysis activity. A third metal binding site may serve a structural role, possibly stabilizing part of the interface between the N-terminal and the catalytic domain.)
subunit: Homohexamer. - Cholesterol-induced leucine aminopeptidase 3 (LAP3) upregulation inhibits cell autophagy in pathogenesis of NAFLD.
Feng, Aging 2022 - GeneRIF: Cholesterol-induced leucine aminopeptidase 3 (LAP3) upregulation inhibits cell autophagy in pathogenesis of NAFLD.
- PPAR beta/gamma mediates the antihypertensive activity of a synbiotic preparation of Bifidobacterium lactis and Lactobacillus acidophilus in spontaneous hypertensive rats
Huang, Heliyon 2024 - “...1.0280 0.5170 47 Q5SGE0 Leucine-rich PPR motif-containing protein LPPRC 16 1.8707 0.0067 0.7516 0.1874 48 Q68FS4 Cytosol aminopeptidase AMPL 20 1.4997 0.0356 0.8790 0.2671 49 Q6Q0N1 Cytosolic non-specific dipeptidase CNDP2 24 1.2942 0.0067 1.0765 0.7875 50 Q5XHZ0 Heat shock protein 75kDa, mitochondrial TRAP1 18 1.6596 0.0305...”
- Carbonylated Proteins as Key Regulators in the Progression of Metabolic Syndrome.
Kitamura, Antioxidants (Basel, Switzerland) 2023 - “...(+) or Down () CP/WKY CP/Lean 905 P04762 Catalase (CAT) 16.9 7 1.46 1.39 989 Q68FS4 Cytosol aminopeptidase (LAP3) 7.9 3 2.39 2.58 999 O08651 D-3-phosphoglycerate dehydrogenase (PHGDH) 15.0 4 1.69 2.24 1598 O88989 Malate dehydrogenase (MDH1) 26.4 7 1.66 1.93 1888 Q9Z0V6 Thioredoxin-dependent peroxide reductase...”
- Red-Fleshed Apples Rich in Anthocyanins and White-Fleshed Apples Modulate the Aorta and Heart Proteome in Hypercholesterolaemic Rats: The AppleCOR Study.
Catalán, Nutrients 2022 - “...IX 1.183 0.0129 P07943 Akr1b1 Aldose reductase 1.244 0.0134 P27605 Hprt1 Hypoxanthine-guanine phosphoribosyltransferase 1.446 0.0137 Q68FS4 Lap3 Cytosol aminopeptidase 1.443 0.0151 Q62651 Ech1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 1.305 0.0152 M0RBJ7 C3 Complement C3 1.563 0.0153 A0A0H2UHI5 Serpina3n Ab1-233 1.809 0.0161 Q07936 Anxa2 Annexin A2 1.182 0.0176 A0A0G2JY31...”
- Prenatal Stress Impairs Postnatal Learning and Memory Development via Disturbance of the cGMP-PKG Pathway and Oxidative Phosphorylation in the Hippocampus of Rats.
Li, Frontiers in molecular neuroscience 2020 - “...A resistant guanine nucleotide exchange factor 1 (Gbf1) Q5XIC6 Proteasome 26S subunit, non-ATPase 12 (Psmd12) Q68FS4 Leucine aminopeptidase 3 (Lap3) Q6AYL4 RIB43A domain with coiled-coils 1 (Ribc1) Q2TGJ4 Zinc finger, DHHC-type containing 15 (Zdhhc15) P12369 Protein kinase cAMP-dependent type 2 regulatory subunit beta (Prkar2b) Q9Z1P2 Actinin,...”
- Global Proteome Changes in the Rat Diaphragm Induced by Endurance Exercise Training
Sollanek, PloS one 2017 - “...0.7 26 0.016 Protein Nars F1LPV0 Nars 0.6 0.1 0.5 0.1 23 0.048 Cytosol aminopeptidase Q68FS4 Lap3 6.2 0.3 5.3 0.4 16 0.000 Ubiquitin carboxyl-terminal hydrolase D3ZVQ0 Usp5 8.5 0.5 7.9 0.7 9 0.045 Microfilament structure Gamma-synuclein Q63544 Sncg 0.6 0.3 0.4 0.1 72 0.006 Protein...”
- Functional proteomic analysis of corticosteroid pharmacodynamics in rat liver: Relationship to hepatic stress, signaling, energy regulation, and drug metabolism.
Ayyar, Journal of proteomics 2017 - “...and various phosphogens (e.g. creatine phosphate) UP Q63270 Aco1 Cytoplasmic aconitate hydratase Iron sensor UP Q68FS4 Lap3 Cytosol aminopeptidase Processing and regulation of turnover of intracellular proteins UP Q6Q0N1 Cndp2 Cytosolic non-specific dipeptidase Hydrolyzes a variety of dipeptides DOWN/UP P80254 Ddt D-dopachrome decarboxylase Tautomerization of D-dopachrome...”
- Systematic revelation of the protective effect and mechanism of Cordycep sinensis on diethylnitrosamine-induced rat hepatocellular carcinoma with proteomics.
Wang, Oncotarget 2016 - “...the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. 12 Cytosol aminopeptidase Q68FS4 6.77 56.51 129(47%) 20 -3.50.2 0.03 Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides 13 Aldehyde...”
- Protein targets of thioacetamide metabolites in rat hepatocytes.
Koen, Chemical research in toxicology 2013 - “...BB, TB , VCN Carboxylesterase 3 (ES-10) P16303 Mt10, Ms10 (1D) MOL, BB Cytosol aminopeptidase Q68FS4 Ms34 (2D) D-dopachrome decarboxylase P80254 Ct1-3, Mt2, 3, Ms3 (1D); Ct109, 112, 114, 116; Mt113-116; Ms112, 114, 117 (2D) BB, TB , TIE Fructose-bisphosphate aldolase B P00884 Ct9 (1D); Ct56,...”
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Q65FE6 Probable cytosol aminopeptidase from Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)
33% identity, 88% coverage
- Shotgun proteomics as a viable approach for biological discovery in the Pacific oyster.
Timmins-Schiffman, Conservation physiology 2013 - “...CGI_10025180 Peptidyl-prolyl cis-trans isomerase P54985 A,B,C,D CGI_10004092 Extracellular superoxide dismutase Q08420 A,B,C,D CGI_10006610 Cytosol aminopeptidase Q65FE6 A,B,C,D CGI_10013347 ATP synthase subunit Q05825 A,B,D CGI_10012330 Tubulin chain P11833 B,C CGI_10000082 Barrier-to-autointegration factor Q6P026 C,D CGI_10010974 Glyceraldehyde-3-phosphate dehydrogenase P56649 A Protein ID for the oyster is given, as...”
I7GKQ8 Macaca fascicularis brain cDNA clone: QmoA-11343, similar to human leucine aminopeptidase 3 (LAP3), mRNA, RefSeq: NM_015907.2 from Macaca fascicularis
42% identity, 63% coverage
P28838 leucyl aminopeptidase (EC 3.4.11.1); membrane alanyl aminopeptidase (EC 3.4.11.2) from Homo sapiens (see 2 papers)
NP_056991 cytosol aminopeptidase from Homo sapiens
42% identity, 60% coverage
- Identification of early prediction biomarkers of severity in patients with severe fever with thrombocytopenia syndrome based on plasma proteomics.
Zhang, Frontiers in microbiology 2025 - Proteome profiling of cutaneous leishmaniasis lesions due to dermotropic Leishmania donovani in Sri Lanka.
Manamperi, Clinical proteomics 2024 - “...2.54253 0.00032 10 COTL1 Coactosin-like protein1 Q14019 Up-regulated 5.028113 0.00034 11 LAP3 Leucine aminopeptidase 3 P28838 Up-regulated 3.705696 0.00034 12 RPL6 Ribosomal protein L6 Q02878 Up-regulated 2.863634 0.00034 13 MSN Moesin P26038 Up-regulated 3.597755 0.00034 14 IVL Involucrin P07476 Up regulated 3.36279 0.00053 15 TYMP Thymidine...”
- Glioblastoma biomarkers in urinary extracellular vesicles reveal the potential for a 'liquid gold' biopsy.
Hallal, British journal of cancer 2024 - “...[ 40 ] P40227 CCT6A T-complex protein 1 subunit zeta X CUSA-EV [ 3 ] P28838 LAP3 Cytosol aminopeptidase X CUSA-EV [ 3 ] P60174 TPI1 Triosephosphate isomerase X in vitro EV marker [ 40 ] Q92542 NCSTN Nicastrin X CUSA-EV [ 3 ] P50991 CCT4...”
- Differential Proteomic Analysis of Human Sperm: A Systematic Review to Identify Candidate Targets to Monitor Sperm Quality.
Corda, The world journal of men's health 2024 - “...35 ]; AZS [ 26 55 ]; Severe OAT [ 38 ] - - - P28838 LAP3 Cytosol aminopeptidase 4 DF [ 45 ]; IVF failure [ 36 ]; Severe OAT [ 38 ]; AZS [ 26 ] - - - P56730 PRSS12 Neurotrypsin 4 AZS...”
- Proteome profiling of cutaneous leishmaniasis lesions due to dermotropic Leishmania donovani in Sri Lanka.
Manamperi, bioRxiv : the preprint server for biology 2024 - “...P61604 Up-regulated 0.00032 10. COTL1 Coactosin-like protein1 Q14019 Up-regulated 0.00034 11. LAP3 Leucine aminopeptidase 3 P28838 Up-regulated 0.00034 12. RPL6 Ribosomal protein L6 Q02878 Up-regulated 0.00034 13. MSN Moesin P26038 Up-regulated 0.00034 14. IVL Involucrin P07476 Up regulated 0.00053 15. TYMP Thymidine phosphorylase P19971 Up-regulated 0.00053...”
- In Silico Exploration of Metabolically Active Peptides as Potential Therapeutic Agents against Amyotrophic Lateral Sclerosis
Fatoki, International journal of molecular sciences 2023 - “...P41143). I: Neprilysin (MME, P08473). J: kappa opioid receptor (OPRK1, P41145). K: leucine aminopeptidase (LAP3, P28838). L: disks large homolog 4 (DLG4, P78352). M: cathepsin (B and K) (CTSB, P07858). N: lipoxin A4 receptor (by homology) (FPR2, P25090). O: NAD-dependent deacetylase sirtuin 1 (SIRT1/SIRT2, Q96EB6/Q8IXJ6). P:...”
- The proteome and phosphoproteome of circulating extracellular vesicle-enriched preparations are associated with characteristic clinical features in type 1 diabetes.
Casu, Frontiers in endocrinology 2023 - “...0.83 5.49E-10 6.53E-07 P30101 PDIA3 2.87 0.5 6.75E-10 6.53E-07 O75533 SF3B1 -2.64 0.52 1.01E-09 6.53E-07 P28838 LAP3 2.72 0.13 1.24E-09 6.53E-07 P61158 ACTR3 2.78 0.25 1.96E-09 8.23E-07 P00747 PLG 2.48 0.32 5.86E-09 2.05E-06 P02745 C1QA 2.73 -0.03 7.70E-09 2.19E-06 P02794 FTH1 -2.8 -0.21 8.33E-09 2.19E-06 Q9BS26...”
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ.
Liu, International journal of molecular sciences 2023 - “...the 487 amino acid sequence of B. taurus LAP (P00727), 32% identity with human leucyl-aminopeptidase (P28838), 36% identity with mouse LAP3 (Q9CPY7), 37% identity with mosquito LAP (W5J5J1) and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37%...”
- “..., C. cinereus (Q8TGE4), Mucor ambiguous ( A0A0C9MT45), mosquito (W5J5J1 ) , mouse (Q9CPY7), human (P28838) and B. taurus (P00727). Asterisk (*): identical amino acid residues in all proteins. Solid boxes: putative catalytic residues. Dashed boxes: residues involved in coordination with the co-catalytic metal ion. Reverse-coloured...”
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- Cholesterol-induced leucine aminopeptidase 3 (LAP3) upregulation inhibits cell autophagy in pathogenesis of NAFLD.
Feng, Aging 2022 - GeneRIF: Cholesterol-induced leucine aminopeptidase 3 (LAP3) upregulation inhibits cell autophagy in pathogenesis of NAFLD.
- Urinary leucine aminopeptidase 3 in population environmentally exposed to airborne arsenic.
Skoczynska, Human & experimental toxicology 2021 (PubMed)- GeneRIF: Urinary leucine aminopeptidase 3 in population environmentally exposed to airborne arsenic.
- Leucine aminopeptidase 3 promotes migration and invasion of breast cancer cells through upregulation of fascin and matrix metalloproteinases-2/9 expression.
Fang, Journal of cellular biochemistry 2019 (PubMed)- GeneRIF: Leucine aminopeptidase 3 promotes migration and invasion of breast cancer cells through upregulation of fascin and matrix metalloproteinases-2/9 expression.
- LAP3 promotes glioma progression by regulating proliferation, migration and invasion of glioma cells.
He, International journal of biological macromolecules 2015 (PubMed)- GeneRIF: LAP3 might be a new prognostic factor and be close correlation with glioma cell growth, migration, invasion.
- Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma.
Zhang, Journal of molecular histology 2014 (PubMed)- GeneRIF: Overexpression of leucine aminopeptidase 3 contributes to malignant development of human esophageal squamous cell carcinoma.
- Expression of leucine aminopeptidase 3 (LAP3) correlates with prognosis and malignant development of human hepatocellular carcinoma (HCC).
Tian, International journal of clinical and experimental pathology 2014 - GeneRIF: our results indicated that up-regulated expression of LAP3 might contribute to the proliferation and metastasis of hepatocellular carcinoma
- Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson, PloS one 2010 - GeneRIF: Observational study of gene-disease association. (HuGE Navigator)
- Reduced activity of the hypertension-associated Lys528Arg mutant of human adipocyte-derived leucine aminopeptidase (A-LAP)/ER-aminopeptidase-1.
Goto, FEBS letters 2006 (PubMed)- GeneRIF: results suggest that Lys528 is important for maximal activity of adipocyte-derived LAP by maintaining the appropriate structure of the substrate pocket of the enzyme
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AMPL_PLAF7 / Q8IL11 Leucine aminopeptidase; PfLAP; M17 leucyl aminopeptidase; PfA-M17; PfM17LAP; EC 3.4.11.1; EC 3.4.13.- from Plasmodium falciparum (isolate 3D7) (see 12 papers)
Q8IL11 leucyl aminopeptidase (EC 3.4.11.1); aminopeptidase Ey (EC 3.4.11.20) from Plasmodium falciparum (see 5 papers)
PF3D7_1446200, XP_001348613 M17 leucyl aminopeptidase from Plasmodium falciparum 3D7
38% identity, 58% coverage
- function: Aminopeptidase which preferentially cleaves leucine residues from the N-terminus of peptides (PubMed:17107951, PubMed:21844374, PubMed:22359643, PubMed:33536500, PubMed:34133730). Also, has some activity towards tryptophan and methionine and to a lesser extent towards phenylalanine (PubMed:17107951, PubMed:22359643, PubMed:34133730). Has very low activity or no activity towards the other amino acids (PubMed:17107951, PubMed:22359643, PubMed:34133730). In addition, cleaves the Cys-Gly dipeptide, probably as part of the glutathione regulation pathway; cleavage only occurs in the presence of Mn(2+) (PubMed:33303633). During the asexual blood stage, plays a role in the final step of host hemoglobin catabolism, by cleaving hemoglobin-derived oligopeptides providing a source of amino acids for the parasite protein synthesis and for the maintenance of osmotic homeostasis (PubMed:34133730). During the asexual blood stage, may also play a role during the ring-trophozoite transition (PubMed:21844374).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: L-cysteinylglycine + H2O = L-cysteine + glycine (RHEA:28783)
cofactor: Zn(2+) (Binds 2 Zn(2+) ions per subunit (PubMed:19408962, PubMed:20133789, PubMed:21844374, PubMed:23713488, PubMed:25299353, PubMed:25645579, PubMed:35691342). Two metal binding sites with different affinities are located in the enzyme active site and can be occupied in vitro by different metals (PubMed:19408962). Site 1 binds metal with low affinity and can be occupied by Zn(2+), Mn(2+), Co(2+) or Mg(2+) (PubMed:19408962). While Zn(2+) has the highest affinity for site 1, catalytic activity is the highest with Mn(2+) or Co(2+) and less with Mg(2+) (PubMed:19408962, PubMed:33303633). Site 2 binds tightly to the metal ion and can be occupied only by Zn(2+) or Co(2+) (PubMed:19408962). A third metal binding site is also present in an inactive conformation of the hexamer; in this conformation only the metal binding sites 1 and 3 are occupied (PubMed:35691342).)
subunit: Homohexamer composed of dimer of trimers (PubMed:17107951, PubMed:33303633, PubMed:35691342). Both the identity and concentration of metal ions available dictate the extent to which oligomerization occurs; Mn(2+) and Co(2+) induces oligomerization, whereas Mg(2+) has no effect, and Zn(2+) causes irreversible protein aggregation in vitro (PubMed:33303633). - Genetic and chemical validation of Plasmodium falciparum aminopeptidase PfA-M17 as a drug target in the hemoglobin digestion pathway
Edgar, eLife 2022 (no snippet) - Piperaquine-resistant PfCRT mutations differentially impact drug transport, hemoglobin catabolism and parasite physiology in Plasmodium falciparum asexual blood stages
Okombo, PLoS pathogens 2022 - “...as compound 6l in [ 57 ]), a putative dual inhibitor of PfA-M17 leucyl aminopeptidase (PF3D7_1446200) and PfA-M1 alanyl aminopeptidase (PF3D7_1311800), with a 4-fold lower K i for inhibition of the former. Both aminopeptidases have been implicated in hydrolyzing peptides released from Hb, with PfA-M17 and...”
- A metal ion-dependent conformational switch modulates activity of the Plasmodium M17 aminopeptidase.
Webb, The Journal of biological chemistry 2022 - GeneRIF: A metal ion-dependent conformational switch modulates activity of the Plasmodium M17 aminopeptidase.
- Identification of sulfenylation patterns in trophozoite stage Plasmodium falciparum using a non-dimedone based probe
Schipper, Molecular and biochemical parasitology 2021 - “...that those proteins have distinct regulatory mechanisms. One of these proteins is M17 leucyl aminopeptidase (PF3D7_1446200). This protein is a known target for the development of antimalarial drugs, as inhibition leads to death of P. falciparum in culture [ 73 ]. The protein catalyzes the release...”
- “...61 PF3D7_1407800 Plasmepsin IV 51.0 47.0% / 48.8% / 43.0% C370 + + FV 62 PF3D7_1446200 M17 leucyl aminopeptidase 67.8 59.0% / 53.9% / 55.4% C121 + + Protein degradation 63 PF3D7_0317000 Proteasome subunit alpha type-3, putative 29.3 46.0% / 42.9% / 39.3% C222 + 64...”
- Overexpression of Plasmodium falciparum M1 Aminopeptidase Promotes an Increase in Intracellular Proteolysis and Modifies the Asexual Erythrocytic Cycle Development
Hoff, Pathogens (Basel, Switzerland) 2021 - “...25 ]. The remaining five enzymes are a post-prolylaminopeptidase (PF14_0015), a prolyl-aminopeptidase (PF14_0517), a leucyl-aminopeptidase (PF3D7_1446200), an aspartyl-aminopeptidase (PF3D7_0932300) and an alanyl-aminopeptidase (PF3D7_1311800). These aminopeptidases act in concert to hydrolyze hemoglobin [ 11 ]. In this work, we studied the M1 alanyl-aminopeptidase of P. falciparum (PfA-M1),...”
- The apicoplast link to fever-survival and artemisinin-resistance in the malaria parasite
Zhang, Nature communications 2021 - “...vacuole proteases and proteins involved in hemoglobin digestion (PM1, PF3D7_1407900; ATCase, PF3D7_1344800; M1AAP, PF3D7_1311800; LAP, PF3D7_1446200; HSP70, PF3D7_0818900). The key thiamin-synthesis enzyme hydroxyethylthiazole kinase (ThzK, PF3D7_1239600) is absent in P. berghei and P. yoelii , malaria-parasites whose rodent hosts do not present fever. c P. falciparum...”
- Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (PfM1AAP) and M17 leucyl aminopeptidase (PfM17LAP)
Mathew, Scientific reports 2021 - “...different malaria parasite fractions To examine the distribution of Pf M1AAP (PF3D7_1311800) and Pf M17LAP (PF3D7_1446200) in blood stage P. falciparum , we prepared subcellular fractions using modifications from the protocol of Saliba et al. 25 . Parasites released from erythrocytes using saponin detergent where rapidly...”
- Fitness Loss under Amino Acid Starvation in Artemisinin-Resistant Plasmodium falciparum Isolates from Cambodia
Bunditvorapoom, Scientific reports 2018 - “...G A 10.85% PF3D7_1116700 DPAP1 NS T233I 6.0310 13 97 14 1893220 C T 7.45% PF3D7_1446200 PfLAP NS V600I 6.3210 11 96 13 2436013 C A 1.28% PF3D7_1360800 Falcilysin NS A224D 4.6310 4 84 Association with fast artemisinin clearance 8 483714 A G 54.06% PF3D7_0809600 C50...”
- More
- Lasso Proteins-Unifying Cysteine Knots and Miniproteins.
Greń, Polymers 2021 - “...details). For example, there are 120 known chain structures of M17 leucyl aminopeptidase (UniProt ID: Q8IL11), but only one of them has an ester bond. The only exception is Myosin heavy chain, skeletal muscle, adult (UniProt ID: P13538), which has 13 chains with an ester bridge...”
P9WHT3 bacterial leucyl aminopeptidase (EC 3.4.11.10) from Mycobacterium tuberculosis (see paper)
Rv2213 leucyl aminopeptidase from Mycobacterium tuberculosis H37Rv
33% identity, 93% coverage
- Impact of the epoxide hydrolase EphD on the metabolism of mycolic acids in mycobacteria
Madacki, The Journal of biological chemistry 2018 - “...M. tuberculosis H37Rv strain. However, because the gene pepB (Rv2213) located in the downstream vicinity of ephD is oriented in the opposite direction, we do...”
- Biochemical Characterization of Isoniazid-resistant Mycobacterium tuberculosis: Can the Analysis of Clonal Strains Reveal Novel Targetable Pathways?
Nieto, Molecular & cellular proteomics : MCP 2018 - “...NADPH-dependent Rv1908c.1 katG Catalase-peroxidase-peroxynitritase T Rv2213 pepB Aminopeptidase Rv2214 ephD Short-chain type dehydrogenase Rv2346 esxO Esat-6...”
- The Activity of a Hexameric M17 Metallo-Aminopeptidase Is Associated With Survival of Mycobacterium tuberculosis
Correa, Frontiers in microbiology 2017 - “...de Gois (Approved protocol: 027/14). Molecular Cloning and Recombinant Protein Expression Mycobacterium tuberculosis LAP gene (Rv2213 1 ) was subcloned into PCR2.1 TOPO vector (Invitrogen) using a PCR amplified product from the M. tuberculosis H37Rv genome. The recombinant PCR2.1 vector was digested to release the gene...”
- “...mediate aminopeptidolytic activities https://merops.sanger.ac.uk/index.shtml . We identified only one copy of a putative LAP gene (Rv2213 4 ), which has thus far not been characterized. Rv2213 is an open reading frame (ORF) that encodes a protein of 515 amino acids. Multiple amino acid sequence alignments revealed...”
- Mycobacterium tuberculosis Proteome Response to Antituberculosis Compounds Reveals Metabolic "Escape" Pathways That Prolong Bacterial Survival
Danelishvili, Antimicrobial agents and chemotherapy 2017 - “...subunit) SdhA Rv3852, histone-like protein Hns Rv2213, cytosol aminopeptidase PepB Rv0777, adenylosuccinate lyase PurB Rv3443c, 50S ribosomal protein...”
- The endothelin system has a significant role in the pathogenesis and progression of Mycobacterium tuberculosis infection
Correa, Infection and immunity 2014 - “...supernatants were probed for a mycobacterial cytoplasmic enzyme, Rv2213, which was not detected (see Fig. S1 in the supplemental material). Thus, despite having...”
- Survival in nuclear waste, extreme resistance, and potential applications gleaned from the genome sequence of Kineococcus radiotolerans SRS30216
Bagwell, PloS one 2008 - “...DNA binding (independent of Aminopeptidase activity) protein required for maintenance of plasmid monomers. PepA DR_0717 Rv2213 Krad_3276 Krad_1149 PinR Putative recombinase PinR DR_C0005 No homologs Krad_4707 Krad_4374 PolA DNA polymerase I PolA DR_1707 No homologs Krad_2951 PolB DNA polymerase II PolB No homologs No homologs No...”
W5J5J1 Cytosol aminopeptidase from Anopheles darlingi
41% identity, 74% coverage
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ.
Liu, International journal of molecular sciences 2023 - “...with human leucyl-aminopeptidase (P28838), 36% identity with mouse LAP3 (Q9CPY7), 37% identity with mosquito LAP (W5J5J1) and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37% identity with Nicotiana tabacum LAP (U3PLK3). However, when the C-terminal domains of...”
- “...thaliana (Q944P7), Nicotiana tabacum (U3PLK3 ) , C. cinereus (Q8TGE4), Mucor ambiguous ( A0A0C9MT45), mosquito (W5J5J1 ) , mouse (Q9CPY7), human (P28838) and B. taurus (P00727). Asterisk (*): identical amino acid residues in all proteins. Solid boxes: putative catalytic residues. Dashed boxes: residues involved in coordination...”
Q9CPY7 Cytosol aminopeptidase from Mus musculus
NP_077754 cytosol aminopeptidase from Mus musculus
41% identity, 60% coverage
- Abnormal Regulation of Mitochondrial Sphingolipids during Aging and Alzheimer's Disease.
Crivelli, ASN neuro 2024 - “...showed dysregulation of the following proteins: Q9D023, P01831, Q9CQA3, P10605, Q61207, Q9JKC6, P18242, P47738, Q8R404, Q9CPY7, Q6P9K8, O35129, Q8R164, and P28798 which were analyzed for gene enrichment ( Figure 3C ). In this case, the analysis underscored the importance of endo-lysosomal biological functions and lipid-binding protein...”
- Peptidomic Analysis Reveals that Novel Peptide LDP2 Protects Against Hepatic Ischemia/Reperfusion Injury.
Bao, Journal of clinical and translational hepatology 2023 - “...of LDP2 Name Protein Uniprot Accession Site Sequence Molecular weight in kDa Isoelectric point LDP2 Q9CPY7 Lap3 319330 AAKLNLPINIIG 1,237 9 LDP, liver-derived peptide. Discussion Hepatic I/R injury has long been considered a problem in the prognosis of liver surgery, particularly liver transplantation. 24 Several methods...”
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ.
Liu, International journal of molecular sciences 2023 - “...B. taurus LAP (P00727), 32% identity with human leucyl-aminopeptidase (P28838), 36% identity with mouse LAP3 (Q9CPY7), 37% identity with mosquito LAP (W5J5J1) and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37% identity with Nicotiana tabacum LAP (U3PLK3)....”
- “...(U3PLK3 ) , C. cinereus (Q8TGE4), Mucor ambiguous ( A0A0C9MT45), mosquito (W5J5J1 ) , mouse (Q9CPY7), human (P28838) and B. taurus (P00727). Asterisk (*): identical amino acid residues in all proteins. Solid boxes: putative catalytic residues. Dashed boxes: residues involved in coordination with the co-catalytic metal...”
- Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide Isomerase.
Zhang, Cellular and molecular gastroenterology and hepatology 2023 - “...Keratin type II cuticular Hb2 4 2 1 Q99N42 Tymp Thymidine phosphorylase 2 1 1 Q9CPY7 Lap3 Cytosol aminopeptidase 2 1 1 Q9CQD1 Rab5a Ras-related protein Rab-5A 5 1 1 Q9CQV8 Ywhab 14-3-3 protein beta/alpha 16 4 1 Q9CWF2 Tubb2b Tubulin beta-2B chain 31 14 1...”
- Plasma Proteomic Changes of Atherosclerosis after Exercise in ApoE Knockout Mice.
Liao, Biology 2022 - “...homolog 1 7.289 0.0220 DYHC1_MOUSE Q9JHU4 Cytoplasmic dynein 1 heavy chain 1 6.976 0.0232 AMPL_MOUSE Q9CPY7 Cytosol aminopeptidase 5.695 0.0125 NRP1_MOUSE P97333 Neuropilin-1 5.124 0.0163 BIP_MOUSE P20029 Endoplasmic reticulum chaperone BiP 4.781 0.0008 BHMT1_MOUSE O35490 Betainehomocysteine S-methyltransferase 1 4.416 0.0157 PLSL_MOUSE Q61233 Plastin-2 3.783 0.0186 ACE_MOUSE...”
- Integrating Proteomes for Lung Tissues and Lavage Reveals Pathways That Link Responses in Allergen-Challenged Mice
Mahood, ACS omega 2021 - “...protein 2 2.69 0.0125 enriched Q9D7Z6 CLCA1 calcium-activated chloride channel regulator 1 2.68 0.0133 enriched Q9CPY7 LAP3 cytosol aminopeptidase 2.44 0.00181 enriched P24452 CAPG macrophage-capping protein 2.39 0.0172 enriched Q61233 LCP1 plastin-2 2.08 0.00692 enriched Q00612 G6PDX glucose-6-phosphate 1-dehydrogenase X 1.81 0.0102 enriched O88342 WDR1 WD...”
- The Proteomic Signature of Intestinal Acute Rejection in the Mouse.
Oltean, Metabolites 2021 - “...death and survival P52480 Pkm Pyruvate kinase isozymes M1/M2 1.35 Allosteric enzyme, Kinase, Transferase Cancer Q9CPY7 Lap3 Cytosol aminopeptidase 1.32 Aminopeptidase, Hydrolase, Protease Cell death and survival Q9Z1Q5 Clic1 Chloride intracellular channel protein 1 1.32 Ion channel Cellular growth and proliferation Q99JG3 Anxa13 Annexin A13 1.31...”
- Dataset on the mass spectrometry-based proteomic profiling of the kidney from wild type and the dystrophic mdx-4cv mouse model of X-linked muscular dystrophy.
Dowling, Data in brief 2020 - “...A subunit beta Mep1b 2 7.1821 0.00095 1.4 P12658 Calbindin Calb1 2 5.1746 0.01903 1.4 Q9CPY7 Cytosol aminopeptidase Lap3 3 10.4237 0.02227 1.4 Q9DCY0 Glycine N-acyltransferase-like protein Keg1 Keg1 2 4.7402 0.00128 1.39 Q9CR67 Transmembrane protein 33 Tmem33 2 6.1302 0.00735 1.38 Q61207 Prosaposin Psap 2...”
- More
- The aminopeptidase LAP3 suppression accelerates myogenic differentiation via the AKT-TFE3 pathway in C2C12 myoblasts.
Osana, Journal of cellular physiology 2023 (PubMed)- GeneRIF: The aminopeptidase LAP3 suppression accelerates myogenic differentiation via the AKT-TFE3 pathway in C2C12 myoblasts.
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity
Guo, Veterinary research 2020 - “...Brugia malayi (CTP81255), Caenorhabditis elegans (NP_498854). Two sequences of Homo sapiens (AAD17527) and Mus musculus (NP_077754) were used as the outgroups. The phylogenetic analysis was performed in MEGA 7.0 based on the Neighbour-joining (NJ) method as described previously [ 25 ]. Cloning and expression of recombinant...”
Q7QFS4 Cytosol aminopeptidase from Anopheles gambiae
40% identity, 72% coverage
TGME49_090670, TGME49_290670 leucyl aminopeptidase LAP from Toxoplasma gondii ME49
41% identity, 44% coverage
- Metallopeptidases of Toxoplasma gondii: in silico identification and gene expression
Escotte-Binet, Parasite (Paris, France) 2018 - “...]. In this in silico study, one LAP was found in the ToxoDB, referenced as TGME49_290670 and previously described by Jia et al. in 2010 [ 25 ]. This exopeptidase is localized in the cytoplasm of parasites and appears to be involved in free amino acid...”
- Extensive lysine acetylation occurs in evolutionarily conserved metabolic pathways and parasite-specific functions during Plasmodium falciparum intraerythrocytic development
Miao, Molecular microbiology 2013 - “...isoleucine-tRNA synthetase, putative PF13_0141 L-lactate dehydrogenase (LDH) TGME49_032350 lactate dehydrogenase PF14_0439 M17 leucyl aminopeptidase (LAP) TGME49_090670 cytosol aminopeptidase PF10_0036 N-acetyltransferase, putative TGME49_019760 N-terminal acetyltransferase complex subunit ARD1, putative PFE0630c orotate phosphoribosyltransferase (OPRT) TGME49_059660 orotate phosphoribosyltransferase, putative PF13_0234 phosphoenolpyruvate carboxykinase (PEPCK) TGME49_089650 phosphoenolpyruvate carboxykinase PFI1105w phosphoglycerate kinase...”
- Chemical genetic screen identifies Toxoplasma DJ-1 as a regulator of parasite secretion, attachment, and invasion
Hall, Proceedings of the National Academy of Sciences of the United States of America 2011 - “...by TOP-ABPP Gene ID TGME49_110640 TGME49_014290 TGME49_090670 TGME49_029000 TGME49_089690 TGME49_047600 Protein Molecular mass (kDa) Modified peptide Spectral...”
- The Hsp90 co-chaperone p23 of Toxoplasma gondii: Identification, functional analysis and dynamic interactome determination
Echeverria, Molecular and biochemical parasitology 2010 - “...TGME49_088360 80.m00063 4 12 nascent polypeptide-associated complex Tz TGME49_005560 20.m03918 3 13 Cytosol aminopeptidase Bz TGME49_090670 80.m00088 4 Kinases/ Phosphorylases/ Phosphatases 14 Rhoptry protein 4 (protein kinase Rop4)) Bz TGME49_095110 83.m02145 9 15 Rhoptry protein 5 (protein kinase Rop5) Bz TGME49_108080 551.m00238 13 16 Uridine Phosphorylase...”
P73971 Probable cytosol aminopeptidase from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
37% identity, 91% coverage
sll2001 leucine aminopeptidase from Synechocystis sp. PCC 6803
37% identity, 88% coverage
Pdsh_04150 leucyl aminopeptidase family protein from Pyrodictium delaneyi
42% identity, 66% coverage
- The draft genome of the hyperthermophilic archaeon Pyrodictium delaneyi strain hulk, an iron and nitrate reducer, reveals the capacity for sulfate reduction
Demey, Standards in genomic sciences 2017 - “...also identified in the genome 13 putative peptidases, including a metallocarboxypeptidase (M32) (Pdsh_06240), three aminopeptidases (Pdsh_04150, Pdsh_05315, and Pdsh_07085), a metalloprotease (M50) (Pdsh_05275), and several omega peptidases (Pdsh_06520, Pdsh_06735, Pdsh_07355, Pdsh_07465, and Pdsh_07745). We also identified prolyl oligopeptidases (Pdsh_07465 and Pdsh_07695), an isoaspartyl aminopeptidase (Pdsh_07355), pyrrolidone-carboxylate...”
MSMEG_4281 cytosol aminopeptidase from Mycobacterium smegmatis str. MC2 155
35% identity, 78% coverage
8ez4C / Q8IL11 Plasmodium falciparum m17 in complex with inhibitor 9aa (see paper)
38% identity, 67% coverage
- Ligands: n-[(1r)-2-(hydroxyamino)-2-oxo-1-(3',4',5'-trifluoro[1,1'-biphenyl]-4-yl)ethyl]-n~2~-phenylglycinamide; carbonate ion; zinc ion (8ez4C)
MPN572 similar to CYTOSOL AMINOPEPTIDASE (LEUCINE AMINOPEPTIDASE) (LAP)) from Mycoplasma pneumoniae M129
P75206 Probable cytosol aminopeptidase from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
39% identity, 76% coverage
- Protein cleavage influences surface protein presentation in Mycoplasma pneumoniae
Berry, Scientific reports 2021 - “...studies identified several surface accessible peptidases including, MPN197 (oligoendopeptidase F homolog), MPN022 (putative proline iminopeptidase), MPN572 (probable cytosol aminopeptidase), MPN470 (putative Xaa-Pro aminopeptidase), and the two aforementioned putative proteases with trypsin-like domains, MPN592 and MPN083. Our combined analysis identified 13 cleavage fragments of MPN052 using LCMS/MS...”
- Protein quality control and regulated proteolysis in the genome-reduced organism Mycoplasma pneumoniae
Burgos, Molecular systems biology 2020 - “...iminopeptidase Pip (MPN022), oligoendopeptidase F PepF (MPN197), XPro aminopeptidase PepP (MPN470), and leucine aminopeptidase PepA (MPN572). Several peptidases and proteases implicated in protein processing and maturation are also present, including the methionine aminopeptidase Map (MPN186), ribosomalprocessing cysteine protease Prp (MPN326), and the lipoprotein signal peptidase Lsp...”
- “...proteases, and peptidases involved in peptide degradation such as MPN022 (Pip), MPN197 (PepF), MPN470 (PepP), MPN572 (PepA), and in protein processing and maturation, such as MPN186 (Map), MPN326 (Prp), and MPN293 (Lsp). Regulation of specific protease and chaperone genes through the MPN124 (HrcA) regulatory system is...”
- Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae
Trussart, Nature communications 2017 - “...E MPN529 ihf Histone-like bacterial DNA-binding protein F MPN554 ssbB Putative single-stranded DNA-binding protein E MPN572 pepA Probable cytosol aminopeptidase (EC 3.4.11.1) (leucine aminopeptidase) (LAP) E MPN608 phoU Transcriptional regulator involved in phosphate transport system E MPN626 mpn626 Alternative sigma factor NE MPN686 dnaA Chromosomal replication...”
- The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases
Schmidl, Molecular & cellular proteomics : MCP 2010 - “...Ldh MPN677 Soj MPN547 MPN555 PepA MPN547 MPN555 MPN572 MPN665 MPN674 MPN677 MPN688 Protein name Locus name TABLE I--continued Phosphoproteome of M. pneumoniae...”
- Transcriptional heat shock response in the smallest known self-replicating cell, Mycoplasma genitalium
Musatovova, Journal of bacteriology 2006 - “...and the gene encoding putative leucine aminopeptidase (lap, MPN572) was detected. The organization of the groESL region in M. pneumoniae is similar to that...”
- New Insights into the Mechanism of Antibacterial Action of Synthetic Peptide Mo-CBP3-PepI against Klebsiella pneumoniae
Branco, Antibiotics (Basel, Switzerland) 2022 - “...histidine kinase HprS P76339 Escherichia coli (strain K12) Plasma membrane 0.213 Unknown Probable cytosol aminopeptidase P75206 Mycoplasma pneumoniae (strain ATCC 29342/M129) Cytoplasm 2.097 Putative acetyltransferase MPN_114 P75448 Mycoplasma pneumoniae (strain ATCC 29342/M129) Unknown 1.703...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...Mycoplasma genitalium (P47631); M.pneu, Mycoplasma pneumoniae (P75206); N.gonor, Neisseria gonorrhoeae (NUMG); P.aPepA, Pseudomonas aeruginosa PepA; P.mPepB, P....”
NCLIV_042660 probable cytosol aminopeptidase, related from Neospora caninum Liverpool
41% identity, 61% coverage
MAB_1948c Probable cytosol aminopeptidase (leucyl aminopeptidase?) from Mycobacterium abscessus ATCC 19977
37% identity, 81% coverage
AMPL_BOVIN / P00727 Cytosol aminopeptidase; Cysteinylglycine-S-conjugate dipeptidase; Leucine aminopeptidase 3; LAP-3; Leucyl aminopeptidase; LAP; Peptidase S; Proline aminopeptidase; Prolyl aminopeptidase; EC 3.4.11.1; EC 3.4.13.23; EC 3.4.11.5 from Bos taurus (Bovine) (see 3 papers)
P00727 leucyl aminopeptidase (EC 3.4.11.1) from Bos taurus (see 4 papers)
NP_776523 cytosol aminopeptidase from Bos taurus
41% identity, 60% coverage
- function: Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides (PubMed:14583094, PubMed:16519517). The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme (PubMed:16519517). For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates (PubMed:16519517). Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status (PubMed:14583094).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: an S-substituted L-cysteinylglycine + H2O = an S-substituted L-cysteine + glycine (RHEA:60444)
catalytic activity: L-cysteinylglycine + H2O = L-cysteine + glycine (RHEA:28783)
catalytic activity: S-benzyl-L-cysteinylglycine + H2O = S-benzyl-L-cysteine + glycine (RHEA:62568)
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Zn(2+) Mn(2+) (Binds two metal ions per subunit. Two metal binding sites with different affinities are located in the enzyme active site and can be occupied in vitro by different metals: site 1 is occupied by Zn(2+), Mn(2+), Mg(2+) or Co(2+), while the tight binding site 2 can be occupied by only Zn(2+) or Co(2+) (PubMed:16519517). One Zn(2+) ion is tightly bound to site 2 and essential for enzyme activity in vivo, while site 1 can be occupied by different metals to give different enzymatic activities (PubMed:16519517). Mn(2+) is required for Cys-Gly hydrolysis activity (PubMed:16519517). A third metal binding site may serve a structural role, possibly stabilizing part of the interface between the N-terminal and the catalytic domain (PubMed:7619821).)
subunit: Homohexamer. - Mechanism of peptide hydrolysis by co-catalytic metal centers containing leucine aminopeptidase enzyme: a DFT approach.
Zhu, Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 2012 (PubMed)- GeneRIF: In this density functional theory study, reaction mechanisms of a co-catalytic binuclear metal center (Zn1-Zn2) containing enzyme leucine aminopeptidase for two different metal bridging nucleophiles (H(2)O and -OH) have been investigated.
- Functional characterization of genetic polymorphisms identified in the promoter region of the bovine PEPS gene.
Ju, DNA and cell biology 2012 - GeneRIF: promoter polymorphism and milk production
- Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits.
Zheng, Molecular biology reports 2011 (PubMed)- GeneRIF: The LAP3 gene possibly contributed to conducting association analysis and can be used as molecular marker in milk production traits and other performance for animal breeding.
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...Mucor ambiguus (A0A0C9MT45), 33% identity with the 487 amino acid sequence of B. taurus LAP (P00727), 32% identity with human leucyl-aminopeptidase (P28838), 36% identity with mouse LAP3 (Q9CPY7), 37% identity with mosquito LAP (W5J5J1) and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with...”
- “...one 1-residue insert, three 1-residue deletions and one 2-residue deletion between bcLAP and bovine LAP (P00727) ( Figure 1 ). The C-terminal domains of the M17 family of leucylaminopeptidases from the Bacillus cereus family of strains were more conservative, with no residue deletion and no insert...”
- Real-time structural motif searching in proteins using an inverted index strategy.
Bittrich, PLoS computational biology 2020 - “...referring to the number of residues. For BLLAP, all 9 structures curated with UniProt ID P00727 in the PDB were detected with this structural motif search. The remaining 48 hits (R.M.S.D. <1 ) represent leucine aminopeptidases from other organisms or leucine-aminopeptidase-like enzymes, such as the bottromycin...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...aeruginosa PepA (AAD04821); B.tLAP, Bos taurus kidney (P00727); A.pLAP, Aeropyrum pernix LAP (NUMG); SynecLAP, Synechocystis sp. LAP (P73971); B.sLAP, Bacillus...”
- “...pertussis (NUMG); B.tLAP, Bos taurus kidney (P00727); C.cLAP2, C. crescentus LAP2; C.jejun, Campylobacter jejuni (NUMG); C.pneum, Chlamydia pneumoniae...”
2ewbA / P00727 The crystal structure of bovine lens leucine aminopeptidase in complex with zofenoprilat (see paper)
41% identity, 63% coverage
- Ligands: zinc ion; carbonate ion; l-proline, 1-[(2s)-3-mercapto-2-methyl-1-oxopropyl]-4-(phenylthio)-, 4s (2ewbA)
PY17X_1313800 chromosome condensation regulator leucine aminopeptidase from Plasmodium yoelii
39% identity, 54% coverage
AMPL1_ARATH / P30184 Leucine aminopeptidase 1; Leucyl aminopeptidase 1; AtLAP1; Proline aminopeptidase 1; Prolyl aminopeptidase 1; EC 3.4.11.1; EC 3.4.11.5 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
AT2G24200 cytosol aminopeptidase from Arabidopsis thaliana
NP_179997 Cytosol aminopeptidase family protein from Arabidopsis thaliana
35% identity, 89% coverage
- function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides (Probable). Possesses leucine aminopeptidase activity against the model substrate leucine- amido methyl coumarin (PubMed:22493451). Possesses Cys-Gly dipeptidase activity. In addition, can cleave Cys-Leu and Leu-Cys dipeptides (PubMed:25716890).
function: Functions as a molecular chaperone to protect proteins from heat-induced damage.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mn(2+) (Binds 2 Mn(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers). - Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families
Pascual, Marine drugs 2023 - “...thaliana ) - M17.A01 Arabidopsis thaliana At4g30910 ( Arabidopsis thaliana ) - M17.A02 Arabidopsis thaliana At2g24200 ( Arabidopsis thaliana ) - M17.A03 Arabidopsis thaliana CG7340 g.p. ( Drosophila melanogaster ) - M17.A04 Drosophila melanogaster ZK353.6 ( Caenorhabditis elegans ) - M17.A05 Caenorhabditis elegans * IUBMB: International...”
- What Antarctic Plants Can Tell Us about Climate Changes: Temperature as a Driver for Metabolic Reprogramming
Bertini, Biomolecules 2021 - “...subtilase family protein 1.42 10 3 1.76 AT4G35090 CAT2, catalase 2 7.64 10 4 1.61 AT2G24200 Cytosol aminopeptidase family protein (ATLAP1, LAP1, LEUCYL AMINOPEPTIDASE 1) 9.74 10 4 1.60 AT5G58070 ATTIL, TIL, temperature-induced lipocalin 5.70 10 3 1.40 AT1G03090 MCCA, methylcrotonyl-CoA carboxylase alpha chain, mitochondrial/3-methylcrotonyl-CoA carboxylase...”
- Function and Regulation of Chloroplast Peroxiredoxin IIE
Dreyer, Antioxidants (Basel, Switzerland) 2021 - “...aldolase 4 AT4G26530 O65581 Fructose-bisphosphate aldolase 5 AT5G49910 Q9LTX9 Heat shock 70 kDa protein 7 AT2G24200 P30184 Leucine aminopeptidase 1 AT5G45930 Q5XF33 Magnesium-chelatase subunit ChII-2 AT1G70890 Q9SSK5 MLP-like protein 43 AT5G26000 P37702 Myrosinase 1 AT3G62030 P34791 Peptidyl-prolyl cis-trans isomerase CYP20-3 AT2G29630 O82392 Phosphomethylpyrimidine synthase AT5G52920 Q9FLW9...”
- Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae
Shimizu, Philosophical transactions of the Royal Society of London. Series B, Biological sciences 2020 - “...38.367 338353 LVEMHFPLPEGRSPSR peak 10 2254.161 15.379 317337 TANFPQIYAVGRAAASRHAPR AspGluAlaAsp (DEAD)-box ATP-dependent RNA helicase CML137C At2g24200 nucleus 66.4 24.4 peak 4 870.56 19.981 165172 VAVLSLLR peak 5 944.552 11.224 391398 LLAEEISK peak 8 1851.882 53.312 5773 IQSVPGVPQELADTLER similar to GTPase-activating protein CMJ230C At4g15850 vesicle 55.0 23.3...”
- Identifying Early Warning Signals for the Sudden Transition from Mild to Severe Tobacco Etch Disease by Dynamical Network Biomarkers
Tarazona, Viruses 2019 - “...20S PROTEASOME BETA SUBUNIT G1 , PBG1 ), At2g16600 ( CYCLOPHILIN 19 , CYP19 ), At2g24200 ( LEUCYL AMINOPEPTIDASE 1 , LAP1 ), At3g02630 ( ACYL ACYL CARRIER PROTEIN (ACP) DESATURASE 5 , AAD5 ), At4g26130 (hypothetical protein described as a cotton fiber protein), At5g2107 (hypothetical...”
- Increases in activity of proteasome and papain-like cysteine protease in Arabidopsis autophagy mutants: back-up compensatory effect or cell-death promoting effect?
Havé, Journal of experimental botany 2018 - “...https://www.ebi.ac.uk/merops/ ), UniProt ( www.uniprot.org/ ), agriGO (bioinfo.cau.edu.cn/agriGO), and Mapman (mapman.gabipd.org/). Accession numbers AT4G38220; AT2G27020; AT2G24200; AT4G20850; AT5G35590; AT4G31300; AT3G22110; AT2G05840; AT4G14800; AT3G60820; AT1G53750; AT3G05530; AT4G17510; AT5G05780; AT5G58290; AT5G10540; AT1G21720; AT1G56450; AT5G42790; AT4G01610; AT1G47128; AT1G53850; AT5G45890; AT5G51070; AT3G13235; AT1G79340; AT1G50380; AT3G51260; AT5G23540; AT4G38630; AT1G51710; AT5G66140; AT4G30910;...”
- “...1.77 1.84 2.21 XICs AT2G27020 PAG1 Thr 20S (CP ) 1.41 1.79 1.64 1.84 XICs AT2G24200 LAP1 Met PM,C 1.44 1.55 1.42 1.41 XICs AT4G20850 TPP2 Ser Pl 1.38 1.48 1.38 1.55 XICs AT5G35590 PAA1 Thr 20S (CP ) 1.24 1.58 1.28 1.63 XICs AT4G31300 PBA1...”
- DYn-2 Based Identification of Arabidopsis Sulfenomes
Akter, Molecular & cellular proteomics : MCP 2015 - “...protein AT1G16350 AT1G09780 AT1G11840 AT5G13520 AT5G60160 AT2G24200 AT3G06650 AT3G06580 AT2G41530 AT5G58330 Tubulin binding cofactor C domain-containing protein...”
- “...Protein degradation AT5G36210 AT5G13520 AT1G22920 AT2G24200 AT1G09210 AT1G56340 Primary metabolism AT3G06650 AT4G24830 AT3G48000 AT1G24180 AT5G44340 AT5G19770...”
- Proteomic analysis of endoplasmic reticulum stress responses in rice seeds
Qian, Scientific reports 2015 - “...4.019 AT1G63800 ubiquitin-conjugating enzyme LOC_Os10g31000 gi|115474297 3.156 AT3G55410 2-oxoglutarate dehydrogenase E1 component LOC_Os07g49520 gi|75261364 4.276 AT2G24200 leucine aminopeptidase LOC_Os02g55140 gi|311893431 6.100 AT4G33150 saccharopine dehydrogenase LOC_Os02g54254 gi|222616995 4.201 AT1G55860 HECT-domain domain containing protein LOC_Os12g24080 gi|37718894 2.670 AT5G15400 U-box domain-containing protein LOC_Os03g31400 gi|125987818 7.325 Cysteine proteinase inhibitor 2...”
- More
- Fruit ripening-associated leucylaminopeptidase with cysteinylglycine dipeptidase activity from durian suggests its involvement in glutathione recycling
Panpetch, BMC plant biology 2021 - “...tree was constructed with MEGA v. 7 using 1000 bootstrap replicates. Arabidopsis thaliana : AtLAP1 (NP_179997), AtLAP2 (NP_194821), and AtLAP3 (NP_001328632); Solanum lycopersicum : SlLAP1 (NP_001233862.2) and SlLAP2 (NP_001233884.2); Solanum tuberosum : StLAP1 (XP_006350102.1) and StLAP2 (XP_015165363.1); Durio zibethinus: Musang King DzLAP1_MK (NW_019167860.1) and DzLAP2_MK (NW_019168159)...”
- Proteome and Interactome Linked to Metabolism, Genetic Information Processing, and Abiotic Stress in Gametophytes of Two Woodferns
Ojosnegros, International journal of molecular sciences 2023 - “...CLP PROTEASE PROTEOLYTIC SUBUNIT-RELATED PROTEIN 2 32.7 4 1 1 4.49 10 126 Degradation 170504-166_2_ORF2 P30184 LAP1 LEUCINE AMINOPEPTIDASE 1 62.5 4 1 4 0...”
- Function and Regulation of Chloroplast Peroxiredoxin IIE
Dreyer, Antioxidants (Basel, Switzerland) 2021 - “...4 AT4G26530 O65581 Fructose-bisphosphate aldolase 5 AT5G49910 Q9LTX9 Heat shock 70 kDa protein 7 AT2G24200 P30184 Leucine aminopeptidase 1 AT5G45930 Q5XF33 Magnesium-chelatase subunit ChII-2 AT1G70890 Q9SSK5 MLP-like protein 43 AT5G26000 P37702 Myrosinase 1 AT3G62030 P34791 Peptidyl-prolyl cis-trans isomerase CYP20-3 AT2G29630 O82392 Phosphomethylpyrimidine synthase AT5G52920 Q9FLW9 Plastidial...”
- Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
Wang, Scientific reports 2017 - “...0.61 4.6E-06 38 B6T451 Importin subunit alpha Zea mays Q96321 N/A 3 0.57 0.00233 39 P30184 Leucine aminopeptidase 1 Arabidopsis thaliana P30184 LAP1 2 2.38 1.2E-08 40 Q9LXC0 GDP dissociation inhibitor Arabidopsis thaliana Q9LXC0 At5g09550 3 2.40 0.00036 41 A7PZL3 Probable polygalacturonase Vitis vinifera Q9SMT3 GSVIVT00026920001...”
- Salt-induced subcellular kinase relocation and seedling susceptibility caused by overexpression of Medicago SIMKK in Arabidopsis
Ovečka, Journal of experimental botany 2014 - “...thiolase 2 peroxisomal 48548 8.35 4 16.88 303.25 476.75 1.57 0.005 Proteolysis and protein processing P30184 Leucine aminopeptidase 1 54475 5.55 6 22.69 296.75 489.75 1.65 0.002 Q9LMU2 Kunitz type trypsin and protease inhibitor domain-containing protein 22067 8.97 4 18.36 1004.66 541.33 0.54 0.001 Lipid binding...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...A. pernix LAP (NUMG); A.thal, Arabidopsis thaliana (P30184); B.sLAP, Bacillus subtilis LAP; B.pert, Bordetella pertussis (NUMG); B.tLAP, Bos taurus kidney...”
NP_001108319 cytosol aminopeptidase from Danio rerio
41% identity, 60% coverage
P47631 Probable cytosol aminopeptidase from Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)
38% identity, 75% coverage
XP_003377703 aminopeptidase A/I from Trichinella spiralis
35% identity, 85% coverage
ETH_00012380 cytosol aminopeptidase, putative from Eimeria tenella
35% identity, 82% coverage
- Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Katrib, BMC genomics 2012 - “...M16 - PDB: Pitrilysin (M16 family) [ Escherichia coli O157:H7] (E=7e-05) None ER4 Leucine aminopeptidase ETH_00012380 on Supercontig_27 MF M17 Toxoplasma gondii ME49 cytosol aminopeptidase, mRNA (E=4e-54) PDB: E. coli Aminopeptidase A (Pepa) (E=1e-53) Complete ER2 O-sialoglycoprotease ETH_00020530 on Supercontig_5 MK M22 Toxoplasma gondii ME49 glycoprotease...”
6wvvF / A5K3U9 Plasmodium vivax m17 leucyl aminopeptidase (see paper)
35% identity, 88% coverage
B4DQG5 cDNA FLJ54122, highly similar to Cytosol aminopeptidase from Homo sapiens
42% identity, 59% coverage
A0A060A630 leucyl aminopeptidase (EC 3.4.11.1) from Acanthamoeba castellanii (see paper)
40% identity, 57% coverage
P28839 Cytosol aminopeptidase from Sus scrofa
40% identity, 60% coverage
U3PLK3 Leucine aminopeptidase from Nicotiana tabacum
35% identity, 87% coverage
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...(P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37% identity with Nicotiana tabacum LAP (U3PLK3). However, when the C-terminal domains of these different aminopeptidases, which contain their respective active sites, were considered, the sequence similarity was relatively higher (39, 43, 39, 39, 40, 39, 45,...”
- “...domain sequences of bcLAP and leucyl-aminopeptidases from Escherichia coli (P68767), Arabidopsis thaliana (Q944P7), Nicotiana tabacum (U3PLK3 ) , C. cinereus (Q8TGE4), Mucor ambiguous ( A0A0C9MT45), mosquito (W5J5J1 ) , mouse (Q9CPY7), human (P28838) and B. taurus (P00727). Asterisk (*): identical amino acid residues in all proteins....”
AMPL_PLAVS / A5K3U9 Leucine aminopeptidase; PvLAP; M17 leucyl aminopeptidase; Pv-M17; EC 3.4.11.1; EC 3.4.13.- from Plasmodium vivax (strain Salvador I) (see 3 papers)
A5K3U9 leucyl aminopeptidase (EC 3.4.11.1) from Plasmodium vivax (see 2 papers)
38% identity, 55% coverage
- function: Aminopeptidase which preferentially cleaves leucine residues from the N-terminus of peptides (PubMed:19931315, PubMed:34133730). Also, has some activity towards tryptophan and methionine and has very low activity towards alanine, arginine, asparagine, phenylalanine and tyrosine (PubMed:19931315, PubMed:34133730). No activity towards histidine, serine, valine, isoleucine, glycine, aspartic acid and glutamic acid (PubMed:34133730). In addition, cleaves the Cys-Gly dipeptide, probably as part of the glutathione regulation pathway; cleavage only occurs in the presence of Mn(2+) (PubMed:33303633). Plays a role in the final step of host hemoglobin catabolism, by cleaving hemoglobin-derived oligopeptides providing a source of amino acids for the parasite protein synthesis and for the maintenance of osmotic homeostasis (PubMed:34133730).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: L-cysteinylglycine + H2O = L-cysteine + glycine (RHEA:28783)
cofactor: Zn(2+) (Binds 2 Zn(2+) ions per subunit (PubMed:33303633). Two metal binding sites with different affinities are located in the enzyme active site and can be occupied in vitro by different metals (PubMed:33303633). Site 1 binds metal with low affinity and can be occupied by Zn(2+), Mn(2+), Co(2+) or Mg(2+) (By similarity). While Zn(2+) has the highest affinity for site 1, catalytic activity is the highest with Mn(2+) or Co(2+) and less with Mg(2+) (PubMed:33303633). Site 2 binds tightly to the metal ion and can be occupied only by Zn(2+) or Co(2+) (By similarity). A third metal binding site is also present in an inactive conformation of the hexamer; in this conformation only the metal binding sites 1 and 3 are occupied (By similarity).)
subunit: Homohexamer composed of dimer of trimers (PubMed:33303633). Both the identity and concentration of metal ions available dictate the extent to which oligomerization occurs; Mn(2+) and Co(2+) induces oligomerization, whereas Mg(2+) has no effect, and Zn(2+) causes irreversible protein aggregation in vitro (PubMed:33303633).
Q6K669 Leucine aminopeptidase 2, chloroplastic from Oryza sativa subsp. japonica
37% identity, 77% coverage
- ITRAQ-based quantitative proteomic analysis of japonica rice seedling during cold stress
Qing, Breeding science 2022 - “...19.11 3.73 O64437 Inositol-3-phosphate synthase 1 4 8.43 2.40 Q84TA3 Leucine aminopeptidase 15 25.20 0.65 Q6K669 Leucine aminopeptidase 2, chloroplastic 27 39.63 1.29 P38419 Lipoxygenase 7, chloroplastic 16 18.94 1.54 Q7XZW5 Malate dehydrogenase 34 85.31 1.64 Q2QM23 Methyl-CpG binding domain containing protein, expressed 13 56.44 0.52...”
- OsVTC1-1 Gene Silencing Promotes a Defense Response in Rice and Enhances Resistance to Magnaporthe oryzae.
Lamanchai, Plants (Basel, Switzerland) 2022 - “...9 13.81 0.00 12.71 13.43 15 Q7XPY5 Putative beta-glucosidase 15 11.39 18.11 10.45 15.45 16 Q6K669 Leucine aminopeptidase 2 0.00 12.06 15.04 17.61 17 Q3EBC8 Endoribonuclease dicer homolog 2 13.73 18.60 14.93 16.27 18 Q0DV66 Pheophorbide a oxygenase 15.81 0.00 14.65 16.10 19 Q0D5R3 Cysteine-rich receptor-like...”
- Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds
Zhang, Frontiers in plant science 2016 (no snippet) - A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and its wild type Mn1 in maize
Silva-Sanchez, Frontiers in plant science 2014 - “...9 2 0.02 M 403 C0P2V1 Simillar to Leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 5.22 5.62 61.20 28.37 1.48 0.76 5 4 3 0.13 Matsushita-Morita et al., 2011 B4F8W6 Simillar to UTPglucose-1-phosphate uridylyltransferase from Zea mays (B6T4R3) 5.3 52.09 4 5 2 0.16 Eimert...”
- Proteomic comparison of basal endosperm in maize miniature1 mutant and its wild-type Mn1
Silva-Sanchez, Frontiers in plant science 2013 - “...Calnexin 0.98 0.08 1.24 0.01 C0P2V1 Similar to leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 0.98 0.05 1.35 0.03 C0HGV5 Enolase 1.01 0.02 1.46 0.08 Q5EUD6 b Protein disulfide isomerase 1.02 0.01 1.32 0.04 B7ZWY9 Citrate synthase 1.02 0.02 1.35 0.03 B4G0S0 Similar to UMP...”
- “...chain 1.22 0.02 1.15 0.03 C0P2V1 Similar to leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 1.23 0.02 1.03 0.06 B4FTP4 Putative uncharacterized protein 1.26 0.01 1.65 0.05 C0PDB6 Similar to 3-N-debenzoyl-2-deoxytaxol N-benzoyltransferase from Zea mays (B6TJ78) 1.27 0.00 1.39 0.08 Q9AXG8 Lipoxygenase 1.28 0.01 1.11...”
7k5kA / A5K3U9 Plasmodium vivax m17 leucyl aminopeptidase pv-m17 (see paper)
38% identity, 63% coverage
- Ligands: carbonate ion; manganese (ii) ion (7k5kA)
Cj0929 aminopeptidase from Campylobacter jejuni subsp. jejuni NCTC 11168
37% identity, 73% coverage
CJJ81176_0936 cytosol aminopeptidase family protein from Campylobacter jejuni subsp. jejuni 81-176
37% identity, 73% coverage
PBANKA_1309900 M17 leucyl aminopeptidase, putative from Plasmodium berghei ANKA
38% identity, 54% coverage
- Mapping the substrate specificity of the Plasmodium M1 and M17 aminopeptidases
Malcolm, The Biochemical journal 2021 - “...and methods Reagents Genes encoding Pb -M1 (PlasmoDB ID: PBANKA_1410300) and Pb -M17 (PlasmoDB ID: PBANKA_1309900) were purchased from GenScript. The synthesized genes were codon optimized for gene expression in Escherichia coli, encoded an in-frame C-terminal hexa-histidine tag and were cloned into pET-21d expression vectors. All...”
XP_021338349 leucyl aminopeptidase from Babesia microti strain RI
41% identity, 61% coverage
- In Silico Survey and Characterization of Babesia microti Functional and Non-Functional Proteases
Florin-Christensen, Pathogens (Basel, Switzerland) 2021 - “...BBM_III03610 MER0393111subfamily M16C non-peptidase homologs COG1026 Cym1 Zn-dependent peptidase, M16 family 54-450 Inactive T M17 XP_021338349 leucyl aminopeptidase BMR1_02g03960 BBM_II03960 MER0340008family M17 unassigned peptidases PRK00913 multifunctional aminopeptidase a154-499 K300,R376 metal ligand(s): A288, D293, D312, H372, Q374 T/H M18 XP_021338536 aminopeptidase BMR1_03g01710 BBM_III01710 MER0340957 MER1122391 aspartyl aminopeptidase...”
Q0KKA6 leucyl aminopeptidase (EC 3.4.11.1) from Haemaphysalis longicornis (see paper)
43% identity, 60% coverage
C5XUF6 Cytosol aminopeptidase domain-containing protein from Sorghum bicolor
39% identity, 64% coverage
MSB_A0207 M17 family metallopeptidase from Mycoplasma leachii PG50
44% identity, 61% coverage
6wvvK / A5K3U9 Plasmodium vivax m17 leucyl aminopeptidase (see paper)
39% identity, 69% coverage
SACOL0945 cytosol aminopeptidase from Staphylococcus aureus subsp. aureus COL
33% identity, 89% coverage
LOC108983616 leucine aminopeptidase 1-like from Juglans regia
38% identity, 65% coverage
- Metabolomics and Transcriptomics Analyses Reveals the Molecular Regulatory Mechanisms of Walnut (Juglans regia L.) Embryos in Response to Shade Treatment
Liang, International journal of molecular sciences 2023 - “...in the shaded group compared to the control group. The gene encoding leucine aminopeptidase 1 (LOC108983616) was significantly downregulated at 90 DAP in the shaded group compared to the control group. The genes encoding glutathione peroxidase (LOC109009198, LOC109009230) and glutathione S-transferase (LOC109019544) were significantly upregulated at...”
- “...vitamin C (pos-M194T54) was positively correlated with glutathione S-transferase L3 (LOC109021119) and leucine aminopeptidase 1 (LOC108983616) levels. L-Glutathione (neg-M306T69) was positively correlated with glutathione S-transferase U8 (LOC109012036). These results show a complex regulatory mechanism occurring between the accumulation of metabolites in walnut embryos and the abundance...”
AMPL2_ARATH / Q944P7 Leucine aminopeptidase 2, chloroplastic; Leucyl aminopeptidase 2; AtLAP2; Proline aminopeptidase 2; Prolyl aminopeptidase 2; EC 3.4.11.1; EC 3.4.11.5 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
AT4G30920 cytosol aminopeptidase family protein from Arabidopsis thaliana
NP_194821 Cytosol aminopeptidase family protein from Arabidopsis thaliana
37% identity, 66% coverage
- function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides (By similarity). Possesses leucine aminopeptidase activity against the model substrate leucine- amido methyl coumarin (PubMed:22493451). Does not seem to possess Cys- Gly dipeptidase activity (PubMed:25716890).
function: Functions as a molecular chaperone to protect proteins from heat-induced damage.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mn(2+) (Binds 2 Mn(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers). - The tomato chloroplast stromal proteome compendium elucidated by leveraging a plastid protein-localization prediction Atlas
Bhattacharya, Frontiers in plant science 2023 - “...( LapA2 ), and Solyc12g10040 ( LapN ). LAP-A proteins are not present in Arabidopsis; At4g30920 is the ortholog to tomato LAP-N . See Table S2 for additional information. D Some Sol Genomics descriptors were updated with NCBI or Arabidopsis gene annotations based on the literature...”
- Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families
Pascual, Marine drugs 2023 - “...M17.016 Helicobacter pylori similar to cytosol aminopeptidase ( Rattus norvegicus ) - M17.950 Rattus norvegicus At4g30920 ( Arabidopsis thaliana ) - M17.A01 Arabidopsis thaliana At4g30910 ( Arabidopsis thaliana ) - M17.A02 Arabidopsis thaliana At2g24200 ( Arabidopsis thaliana ) - M17.A03 Arabidopsis thaliana CG7340 g.p. ( Drosophila...”
- Protein-protein interactions in plant antioxidant defense
Melicher, Frontiers in plant science 2022 - “...cytoplasm, peroxisome IP/MS (H) Li etal., 2017b IP/WB (L) CAT activity assay (L) BiFC (L) AT4G30920 LAP2, leucyl aminopeptidase 2 senescence, stress response and amino acid turnover cytoplasm, chloroplast Y2H (L) Zhang etal., 2021 BiFC (L) IP/WB (L) SLC (L) CAT2/3 AT5G35410 SOS2, salt overly sensitive...”
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms in Arabidopsis Thaliana
Willems, Frontiers in plant science 2021 - “...2 -ML AT5G46210 Cullin4 (CUL4) Met26 NH 2 /Ac-MK AT2G45170 Autophagy 8E (ATG8E) Met9 Ac-MD AT4G30920 Leucine aminopeptidase 2 (LAP2) Met57 NH 2 /Ac-A AT1G76140 Prolyl endopeptidase Met65 Ac-G Reduction-oxidation processes AT1G60950 Ferredoxin-2 (FD2) Met52 NH 2 -A AT2G17420 NADPH-dependent TRX reductase A (NTRA) Met49 Ac-ME...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...14 O80860, At2g30950 Chaperone protein ClpC2 8, 9 Q9SXJ7, At3g48870 Leucine aminopeptidase 2 47 Q944P7, At4g30920 Leucine aminopeptidase 3 47 Q8RX72, At4g30910 Folding Chaperonin 60 subunit alpha 1 22, 24, 25, 27, 28, 30, 49 P21238, At2g28000 Chaperonin 60 subunit beta 2 22, 23, 28, 89...”
- iTRAQ-Based Quantitative Proteomic Analysis Reveals Cold Responsive Proteins Involved in Leaf Senescence in Upland Cotton (Gossypium hirsutum L.)
Zheng, International journal of molecular sciences 2017 - “...differentially accumulated for the regulation of programmed cell death (PCD) and aging (AT5G60360, AT5G60360, and AT4G30920), as shown in Table S6 , and they may be direct mediators of premature leaf senescence. More importantly, the functions of 12 proteins were verified on the basis of relevant...”
- Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses
Lin, Plant & cell physiology 2016 - “...Table 3 ) ( Floyd and Bowman 2004 ), and Mpo-miR390 targets a LEUCYL AMINOPEPTIDASE2 (AT4G30920) homolog-LW5937 in M. polymorpha (48 degradome reads at the cleavage sites) ( Fig. 4 C, panel ii; Fig. 4 D, panel ii; Table 3 ). Notably, TAS3 -like sequence has...”
- “...Function miRNA Precursor ID LW6010 Mp C3HDZ1 AT1G52150 Homeobox-leucine zipper family Mpo-miR166a LW8433 LW5937 NA AT4G30920 Leucine aminopeptidase chloroplastic-like Mpo-miR390 LW10802 LW5678 NA AT2G39260 RNA binding LW1323 NA CAN66130 NA a Mpo-miR529c.1 LW4860 LW7434 NA AT4G16210 Enoyl-CoA hydratase/isomerase A LW8291 NA AT5G56110 R2R3 MYB transcription factor...”
- Expression analysis of drought stress specific genes in Peanut (Arachis hypogaea , L.)
Pruthvi, Physiology and molecular biology of plants : an international journal of functional plant biology 2013 - “...AhDR 185-Aldehyde reductase (At1g10310); AhDR 76-Proline amino peptidase (At4g30920) b. Seedling level: MILD (60-70% FC) 100 90 80 70 60 50 40 30 20...”
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- Fruit ripening-associated leucylaminopeptidase with cysteinylglycine dipeptidase activity from durian suggests its involvement in glutathione recycling
Panpetch, BMC plant biology 2021 - “...constructed with MEGA v. 7 using 1000 bootstrap replicates. Arabidopsis thaliana : AtLAP1 (NP_179997), AtLAP2 (NP_194821), and AtLAP3 (NP_001328632); Solanum lycopersicum : SlLAP1 (NP_001233862.2) and SlLAP2 (NP_001233884.2); Solanum tuberosum : StLAP1 (XP_006350102.1) and StLAP2 (XP_015165363.1); Durio zibethinus: Musang King DzLAP1_MK (NW_019167860.1) and DzLAP2_MK (NW_019168159) and Chanee...”
- A multi-step peptidolytic cascade for amino acid recovery in chloroplasts.
Teixeira, Nature chemical biology 2017 (PubMed)- GeneRIF: M17.20 (AT4G30920) is specific towards non-polar and aromatic amino acids; is dually-targeted to mitochondria and chloroplasts; in chloroplasts M17.20 plays a role in amino acid recovery as a part of a multi-step peptidolytic cascade. [M17.20]
- The Arabidopsis aminopeptidase LAP2 regulates plant growth, leaf longevity and stress response.
Waditee-Sirisattha, The New phytologist 2011 (PubMed)- GeneRIF: LAP2 (AT4G30920) influences cell wall metabolism and components of cell growth, photosynthesis, and nitrogen metabolism. Loss of function accelerates leaf senescence and renders plants more sensitive to various stresses.
- Role of the Arabidopsis leucine aminopeptidase 2.
Waditee-Sirisattha, Plant signaling & behavior 2011 - GeneRIF: This work indicates an important in planta role for AtLAP2 contributing to a further understanding of the proteases having several implications in higher plants.
- Data-mining approaches reveal hidden families of proteases in the genome of malaria parasite
Wu, Genome research 2003 - “...PF10_0299 M24A AMPM PFE1360c AMPM MAL8P1.140 NP_194821 (Arabidopsis AMPL) AAL16034 (Coccidioides immitis, DNPE) NP_194003 (Arabidopsis GCP) AAG33975...”
- Expression, Characterisation, Homology Modelling and Molecular Docking of a Novel M17 Family Leucyl-Aminopeptidase from Bacillus cereus CZ
Liu, International journal of molecular sciences 2023 - “...and 37% identity with E. coli cytosolic aminopeptidase (P68767), 36% identity with Arabidopsis LAP 2 (Q944P7) and 37% identity with Nicotiana tabacum LAP (U3PLK3). However, when the C-terminal domains of these different aminopeptidases, which contain their respective active sites, were considered, the sequence similarity was relatively...”
- “...three 1-residue deletions, two 1-residue insertions and one 5-residue insert between bcLAP and plant LAP (Q944P7), there was one 1-residue insert, three 1-residue deletions and one 2-residue deletion between bcLAP and bovine LAP (P00727) ( Figure 1 ). The C-terminal domains of the M17 family of...”
- Physiological and Transcriptional Responses of Apocynum venetum to Salt Stress at the Seed Germination Stage
Li, International journal of molecular sciences 2023 - “...Crustapain 10.16 SMAC_06893 D1ZSU8 Extracellular metalloprotease SMAC_06893 6.78 GGP5 O82225 Gamma-glutamyl peptidase 5 4.19 LAP2 Q944P7 Leucine aminopeptidase 2, chloroplastic 3.76 Pcsk2 P21661 Neuroendocrine convertase 2 5.62 maoI Q07121 Primary amine oxidase 3.53 RD19C Q9SUL1 Probable cysteine protease RD19C 5.48 RD21B Q9FMH8 Probable cysteine protease RD21B...”
- Moonlighting protein prediction using physico-chemical and evolutional properties via machine learning methods
Shirafkan, BMC bioinformatics 2021 - “...is provided in Additional file 7 . These proteins are most likely moonlighting. For example, Q944P7 protein, which is referred to as moonlighting in [ 35 ], in addition to peptidase activity, also has chaperone activity, which is independent of peptidase function. List of moonlighting candidates...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...2 14 O80860, At2g30950 Chaperone protein ClpC2 8, 9 Q9SXJ7, At3g48870 Leucine aminopeptidase 2 47 Q944P7, At4g30920 Leucine aminopeptidase 3 47 Q8RX72, At4g30910 Folding Chaperonin 60 subunit alpha 1 22, 24, 25, 27, 28, 30, 49 P21238, At2g28000 Chaperonin 60 subunit beta 2 22, 23, 28,...”
- Physiological and iTRAQ-based proteomic analyses reveal the function of exogenous γ-aminobutyric acid (GABA) in improving tea plant (Camellia sinensis L.) tolerance at cold temperature
Zhu, BMC plant biology 2019 - “...(Q9ZU52) had the same expression patterns; and proteins Aldolase superfamily protein (F4IC59), Leucine aminopeptidase 2 (Q944P7), Thiol protease aleurain-like (Q8RWQ9), Reactive Intermediate Deaminase A (Q94JQ4), Proteasome subunit alpha type-2-B (Q8L4A7) showed no significant differences, but had lower expression in T4 than T3. No proteins showed great...”
- Identification of Moonlighting Proteins in Genomes Using Text Mining Techniques
Jain, Proteomics 2018 - “...leucine aminopeptidase 2 The first example is leucine aminopeptidase 2 (LAP2) from Arabidopsis (UniProtKB ID: Q944P7). It is a di-zine metallopeptidase that catalyzes the cleavage of amino acids from N-terminal of various peptides. In the paper by Scranton et al. , the peptidase activity of LAP2...”
- “...F1 Ref. for F2 Conf.class Number of Pfam domains Arabidopsis thaliana Leucine aminopeptidase 2, chloroplastic Q944P7 Molecular chaperones Leucine aminopeptidase activity, role in insect defense [ 27 ] [ 27 ] 1 1 BTB/POZ and TAZ domain-containing protein 2 Q94BN0 component of the TAC1-mediated telomerase activation...”
- Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper
Fang, Scientific reports 2016 - “...0.880.21 Q9C5U8 Histidinol dehydrogenase (HDH) 143.07 3 466 5.34 47.05 1 1.830.12 0.530.22 0.860.11 0.900.10 Q944P7 Leucine aminopeptidase 3 132.21 2 583 5.66 53.67 1 0.980.11 1.580.26 1.990.27 2.150.25 Q9SIT1 Leucine-rich repeat receptor-like protein kinase 162.50 2 943 6.35 63.23 1 3.210.19 0.680.07 0.480.09 0.980.21 Q9LXL8...”
SMc02825 PUTATIVE AMINOPEPTIDASE PROTEIN from Sinorhizobium meliloti 1021
38% identity, 72% coverage
V7AYA6 Cytosol aminopeptidase domain-containing protein from Phaseolus vulgaris
35% identity, 80% coverage
B9HB22 Cytosol aminopeptidase domain-containing protein from Populus trichocarpa
35% identity, 95% coverage
SA0803 probable cytosol aminopeptidase from Staphylococcus aureus subsp. aureus N315
33% identity, 89% coverage
XP_002529380 leucine aminopeptidase 1 isoform X1 from Ricinus communis
35% identity, 78% coverage
LOC111299369 leucine aminopeptidase 1-like from Durio zibethinus
38% identity, 65% coverage
WP_042363020 M17 family metallopeptidase from Staphylococcus xylosus
37% identity, 68% coverage
XP_002276114 leucine aminopeptidase 1 from Vitis vinifera
37% identity, 65% coverage
SXYL_01980 M17 family metallopeptidase from Staphylococcus xylosus
37% identity, 68% coverage
- Insight into the Genome of Staphylococcus xylosus, a Ubiquitous Species Well Adapted to Meat Products
Leroy, Microorganisms 2017 - “...SXYL_01078, SXYL_01082, SXYL_01120 ( pepA ), SXYL_01136, SXYL_01247-48 , SXYL_01324, SXYL_01348, SXYL_01489, SXYL_01511, SXYL_01806, SXYL_01931, SXYL_01980 ( ampA ), SXYL_02073, SXYL_02105 ( pepT ) Arginine catabolism SXYL_00252 ( arcB ), SXYL_02488 ( arcC ), SXYL_00769 (arg ), SXYL_00290-97 ( ureDGFECBA ) Glutamate catabolism SXYL_02518 ( gltT...”
- Transcriptomic analysis of Staphylococcus xylosus in the presence of nitrate and nitrite in meat reveals its response to nitrosative stress
Vermassen, Frontiers in microbiology 2014 - “...0.6 SXYL_02450 Arginine/lysine/ornithine decarboxylase 0.7 SXYL_01961 argG Argininosuccinate synthase 1.6 SXYL_01373 asnA L-asparaginase 1.9 Peptidase SXYL_01980 ampA Cytosol aminopeptidase 1.9 SXYL_01931 Oligoendopeptidase F 1.7 SXYL_01348 Peptidase T-like protein 1.6 SXYL_00377 dapE Probable succinyl-diaminopimelate desuccinylase 2.3 Glycine, betaine, carnitine, choline SXYL_00488 opuCA Glycine betaine/carnitine/choline ABC transporter 0.3...”
UU507 cytosol aminopeptidase (leucine aminopeptidase) from Ureaplasma parvum serovar 3 str. ATCC 700970
41% identity, 58% coverage
FTT_1094c cytosol aminopeptidase family protein from Francisella tularensis subsp. tularensis SCHU S4
34% identity, 68% coverage
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...Aminopeptidase A, pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B) Glutamate-CSH ligase, gshA # [EC:6.3.2.2] (K01919) FTT_0367c FTN_0277 FTL_1304 Glutathione...”
- “...( Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase B FTN_0780; FTT_1094c; FTL_1108 (DA461685). 1.5 No data No Change at 4/8h. Not essential ( Ramseyetal., 2020 ) Not essential ( Ramseyetal., 2020 ) No effect reported ( Ireland etal., 2019 ) pepN...”
4ksiA / Q10712 Crystal structure analysis of the acidic leucine aminopeptidase of tomato (see paper)
35% identity, 89% coverage
- Ligand: magnesium ion (4ksiA)
O25294 bacterial leucyl aminopeptidase (EC 3.4.11.10) from Helicobacter pylori (see paper)
HP0570 aminopeptidase a/i (pepA) from Helicobacter pylori 26695
40% identity, 64% coverage
- Outer Membrane Vesicles Secreted by Helicobacter pylori Transmitting Gastric Pathogenic Virulence Factors
Wei, ACS omega 2022 - “...P94845 glnA 19 P55988 atpD 20 P55969 hpaA 21 O25806 rpoBC 22 O25743 Ggt 23 O25294 pepA 24 O25017 HP_0231 25 P56116 htpG 26 P55981 vacA 27 O25284 HP_0558 28 P21762 ahpC 29 P52093 ftnA 30 P14916 ureA 31 P71404 clpB 32 O25011 msrAB 33 P56149...”
- “...P56002 fusA 28 O25017 HP_0231 29 O25311 HP_0589 30 O25825 HP_1227 31 O25321 HP_0599 32 O25294 pepA 33 O26083 HP_1562 34 O25423 HP_0721 35 P14916 ureA 36 O06913 frdA 37 O25732 HP_1104 38 O25284 HP_0558 39 O25052 AddB 40 P56008 rpsA 41 O25015 Omp6 42 O25786...”
- Structural and functional aspects of the Helicobacter pylori secretome
Zanotti, World journal of gastroenterology 2014 - “...O25728 Q7X5D6 O25345 O25742 O25749 z b', z z a, f, q, r, z O25294 O25371 O25656 O25663 b' z O25681 O25905 z a, q, v, y y z b' b', z z z b', y z b' 1E9Y, 1E9Z...”
- Cloning, purification and preliminary crystallographic analysis of the Helicobacter pylori leucyl aminopeptidase-bestatin complex
Modak, Acta crystallographica. Section F, Structural biology and crystallization communications 2013 - “...and overexpression The coding sequence for LAP (UniProtKB ID O25294) was PCRamplified from genomic DNA of strain 26695 of H. pylori using Pfu DNA polymerase...”
- Structural and functional aspects of the Helicobacter pylori secretome
Zanotti, World journal of gastroenterology 2014 - “...X HP1124 Tetratricopeptide-like repeat protein Proteases HP0570 Aminopeptidase, PepA HP0657 Processing protease (YmxG) HP1012 Putative Zinc protease HP1018/...”
- “...proteolysis and the intercellular penetration of H. pylori[119]. HP0570 is also predicted to be an aminopeptidase and is enriched in the extracellular...”
- Proteomic analysis of the function of sigma factor σ54 in Helicobacter pylori survival with nutrition deficiency stress in vitro
Sun, PloS one 2013 - “...synthesis 15 frr HP1256 +3.23 16 frr HP1256 +7.87 Protein fate Glutathione metabolism 9 pepA HP0570 -3.34 Protein stability 25 SP:P16436 HP0404 -2.56 Antioxidant proteins Thioredoxin, glutaredoxin, and glutathione 23 trx2 HP1458 -2.56 26 trx2 HP1458 -2.30 27 trx2 HP1458 -4.55 Detoxification 17 napA HP0243 Absent...”
- Targeted identification of glycosylated proteins in the gastric pathogen Helicobacter pylori (Hp)
Champasa, Molecular & cellular proteomics : MCP 2013 - “...In contrast, the mock-enriched control that lacked the azide contained only two Hp proteins (HP0721, HP0570), both of which were also present in the azide-labeled sample. These proteins were subtracted from the list of putative glycoproteins identified in the azide-labeled sample, narrowing the pool of candidate...”
- Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen
O'Toole, BMC genomics 2010 - “...HP0243 NapA Neutrophil activating protein HMU1269 9 HP1286 - Conserved hypo. secreted protein HMU0209 78 HP0570 PepA Aminopeptidase A HMU652 167 HP1205 TufB Elongation factor EF-Tu HMU0312 8 a. H. pylori annotation; b. Cytosolic fraction The abundant members of the cell envelope proteome include proteins involved...”
- Differences in genome content among Helicobacter pylori isolates from patients with gastritis, duodenal ulcer, or gastric cancer reveal novel disease-associated genes
Romo-González, Infection and immunity 2009 - “...proteins (cations) Cell envelope N/A, HP0461, N/A pepA, HP0570, JHP0517 aspS, HP0617, JHP0560 N/A, HP0629, JHP0572 N/A, HP0698, N/A N/A, HP0704, N/A fecA,...”
- Growth phase-dependent response of Helicobacter pylori to iron starvation
Merrell, Infection and immunity 2003 - “...2.3 3.9 2.6 2.1 4.1 Translation HP0403 HP0570 *HP1012 JHP0978 JHP0517 JHP0411 Phenylalanyl-tRNA synthetase, alpha subunit, pheS Aminopeptidase a/I, pepA...”
- Acid-adaptive genes of Helicobacter pylori
Wen, Infection and immunity 2003 - “...1.13 2.33 2.79 2.43 2.37 3.08 HP0382 HP0570 HP0786 HP0793 Zinc-metalloprotease (YJR117W) Aminopeptidase a/i (pepA) Preprotein translocase subunit (secA)...”
- Acid-induced gene expression in Helicobacter pylori: study in genomic scale by microarray
Ang, Infection and immunity 2001 - “...(1.2) ........cag pathogenicity island protein (cag26) HP0570 (0.9) ........Aminopeptidase a/i (pepA) HP0558 (1.1) ........Beta ketoacyl-acyl carrier protein...”
FTN_0780 cytosol aminopeptidase from Francisella tularensis subsp. novicida U112
34% identity, 68% coverage
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...A, pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B) Glutamate-CSH ligase, gshA # [EC:6.3.2.2] (K01919) FTT_0367c FTN_0277 FTL_1304 Glutathione synthetase,...”
- “...essential ( Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase B FTN_0780; FTT_1094c; FTL_1108 (DA461685). 1.5 No data No Change at 4/8h. Not essential ( Ramseyetal., 2020 ) Not essential ( Ramseyetal., 2020 ) No effect reported ( Ireland etal., 2019 )...”
AMPL1_SOLLC / Q10712 Leucine aminopeptidase A1, chloroplastic; LapA1; DR57; Leucyl aminopeptidase 1; LAP 1; Proline aminopeptidase 1; Prolyl aminopeptidase 1; EC 3.4.11.1; EC 3.4.11.5 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see 9 papers)
NP_001233862 leucine aminopeptidase 1, chloroplastic from Solanum lycopersicum
35% identity, 80% coverage
- function: Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (PubMed:11895433, PubMed:12746529). When associated as homohexamer, catalyzes the proteolyzes of Xaa-Leu dipeptides (PubMed:11895433, PubMed:12746529). Possesses leucine aminopeptidase activity against the model substrate leucine-amido methyl coumarin (PubMed:22493451). Presumably involved in the processing and regular turnover of intracellular proteins (Probable). Regulates wound signaling and has a role in insect defense (Probable).
function: Functions as a molecular chaperone to protect proteins from heat-induced damage.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mg(2+) (Binds 2 Mg(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers).
disruption phenotype: No visible phenotype on plants, flowers and fruits development, as well as normal defense responses to P.syringae pv. tomato and no impact on insect growth (e.g. larvae of M.sexta). - Structural insights into chaperone-activity enhancement by a K354E mutation in tomato acidic leucine aminopeptidase.
DuPrez, Acta crystallographica. Section D, Structural biology 2016 (PubMed)- GeneRIF: The crystal structure of the K354E mutant of LAP-A1 was determined at 2.15 A resolution. The structure reveals that the K354E mutation destabilizes an active-site loop and causes significant rearrangement of active-site residues, leading to loss of the catalytic metal-ion coordination required for the peptidase activity.
- Color-Specific Recovery to Extreme High-Light Stress in Plants
Parrine, Life (Basel, Switzerland) 2021 - “...blue light treatment dataset. Sample Name Protein Accession Description Present in BLT and RLT Regular Q10712 Leucine aminopeptidase 1, chloroplastic X P25306 Threonine dehydratase biosynthetic, chloroplastic X K4CVX0 Uncharacterized protein X Q5UNS1 Arginase 2 X K4CVX6 Uncharacterized protein X Burned K4ATA4 Uncharacterized protein X Limit Q10712...”
- Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast⁻Chloroplast Proteomics.
Zhao, International journal of molecular sciences 2019 - “...K4CNE8 ATP-dependent Clp protease proteolytic subunit GO:0000302; GO:0004252; GO:0009570; GO:0009941; GO:0010468 K4B858 Uncharacterized protein GO:0005622 Q10712 Leucine aminopeptidase 1 GO:0004177; GO:0008235; GO:0009507; GO:0030145 K4B3J8 Uncharacterized protein GO:0008836; GO:0009089 K4ASX2 Uncharacterized protein GO:0005623; GO:0045454; GO:0051920 P37222 NADP-dependent malic enzyme GO:0004471; GO:0004473; GO:0006108; GO:0008948; GO:0009507; GO:0046872; GO:0051287 A0A140G1U2...”
- Proteomics and transcriptomics of the BABA-induced resistance response in potato using a novel functional annotation approach
Bengtsson, BMC genomics 2014 - “...17 Resistance gene-like Q93YA6 17 Basic 30kDa endochitinase P400015454 14 Leucine aminopeptidase 1, chloroplastic (DR57) Q10712 13 Pathogen-and wound-inducible antifungal protein CBP20 P400033771 1.3 Glucan endo-1,3-beta-D-glucosidase P400018563 1.2 Strictosidine synthase P400030201 1.1 Endochitinase P400002757 0.9 Miraculin P400018006 -1.1 Kunitz trypsin inhibitor P400046980 -16 Peptidyl-prolyl cis-trans isomerase...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...esculentum (Q42876); L.escuChl, L. esculentum Chloroplast (Q10712); L.pLAP, L. pneumophila LAP; M.lepr, Mycobacterium leprae (CAB11379); M.tube, Mycobacterium...”
4zlaA / O25294 Bestatin complex structure of leucine aminopeptidase from helicobacter pylori (see paper)
40% identity, 64% coverage
- Ligands: zinc ion; sodium ion; bicarbonate ion; 2-(3-amino-2-hydroxy-4-phenyl-butyrylamino)-4-methyl-pentanoic acid (4zlaA)
jhp0517 AMINOPEPTIDASE from Helicobacter pylori J99
40% identity, 64% coverage
- Differences in genome content among Helicobacter pylori isolates from patients with gastritis, duodenal ulcer, or gastric cancer reveal novel disease-associated genes
Romo-González, Infection and immunity 2009 - “...(cations) Cell envelope N/A, HP0461, N/A pepA, HP0570, JHP0517 aspS, HP0617, JHP0560 N/A, HP0629, JHP0572 N/A, HP0698, N/A N/A, HP0704, N/A fecA, fecA_2,...”
- Growth phase-dependent response of Helicobacter pylori to iron starvation
Merrell, Infection and immunity 2003 - “...2.1 4.1 Translation HP0403 HP0570 *HP1012 JHP0978 JHP0517 JHP0411 Phenylalanyl-tRNA synthetase, alpha subunit, pheS Aminopeptidase a/I, pepA Putative zinc...”
AMPL3_ARATH / Q8RX72 Leucine aminopeptidase 3, chloroplastic; Leucyl aminopeptidase 3; AtLAP3; Proline aminopeptidase 3; Prolyl aminopeptidase 3; EC 3.4.11.1; EC 3.4.11.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
NP_194820 Cytosol aminopeptidase family protein from Arabidopsis thaliana
AT4G30910 cytosol aminopeptidase family protein from Arabidopsis thaliana
36% identity, 66% coverage
- function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N- terminal amino acids from various peptides (By similarity). Possesses Cys-Gly dipeptidase activity (PubMed:25716890).
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mn(2+) (Binds 2 Mn(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers). - Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...ClpC2 8, 9 Q9SXJ7, At3g48870 Leucine aminopeptidase 2 47 Q944P7, At4g30920 Leucine aminopeptidase 3 47 Q8RX72, At4g30910 Folding Chaperonin 60 subunit alpha 1 22, 24, 25, 27, 28, 30, 49 P21238, At2g28000 Chaperonin 60 subunit beta 2 22, 23, 28, 89 Q9LJE4, At3g13470 Chaperonin 60 subunit...”
- A multi-step peptidolytic cascade for amino acid recovery in chloroplasts.
Teixeira, Nature chemical biology 2017 (PubMed)- GeneRIF: M17-10 (AT4G30910) is dually targeted to mitochondria and chloroplasts. [M17-10]
- Marine Invertebrates: A Promissory Still Unexplored Source of Inhibitors of Biomedically Relevant Metallo Aminopeptidases Belonging to the M1 and M17 Families
Pascual, Marine drugs 2023 - “...norvegicus ) - M17.950 Rattus norvegicus At4g30920 ( Arabidopsis thaliana ) - M17.A01 Arabidopsis thaliana At4g30910 ( Arabidopsis thaliana ) - M17.A02 Arabidopsis thaliana At2g24200 ( Arabidopsis thaliana ) - M17.A03 Arabidopsis thaliana CG7340 g.p. ( Drosophila melanogaster ) - M17.A04 Drosophila melanogaster ZK353.6 ( Caenorhabditis...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...8, 9 Q9SXJ7, At3g48870 Leucine aminopeptidase 2 47 Q944P7, At4g30920 Leucine aminopeptidase 3 47 Q8RX72, At4g30910 Folding Chaperonin 60 subunit alpha 1 22, 24, 25, 27, 28, 30, 49 P21238, At2g28000 Chaperonin 60 subunit beta 2 22, 23, 28, 89 Q9LJE4, At3g13470 Chaperonin 60 subunit beta...”
- Increases in activity of proteasome and papain-like cysteine protease in Arabidopsis autophagy mutants: back-up compensatory effect or cell-death promoting effect?
Havé, Journal of experimental botany 2018 - “...AT1G56450; AT5G42790; AT4G01610; AT1G47128; AT1G53850; AT5G45890; AT5G51070; AT3G13235; AT1G79340; AT1G50380; AT3G51260; AT5G23540; AT4G38630; AT1G51710; AT5G66140; AT4G30910; AT4G39090; AT5G43060; AT5G10760; AT1G16470; AT1G79210; AT2G14260; AT5G23140; AT4G16190; AT5G26860; AT3G14290; AT3G20630; AT2G41790; AT4G36760; AT5G36210; AT1G50250; AT5G42270; AT4G36195; AT1G09750; AT3G54400; AT3G02110; AT2G39850; AT1G52510; AT2G47940; AT2G35780; AT5G39830; AT3G27925; AT3G14067; AT3G19170; AT5G42390; AT3G24590;...”
- “...1.22 1.06 1.05 SC AT5G66140 PAD2 Thr 20S (CP ) 1.25 5.00 2.33 1.67 SC AT4G30910 LAP3 Met Pl 0.89 1.35 1.20 1.45 XICs AT4G39090 RD19A Cys V,N 1.15 1.74 0.96 1.56 XICs AT5G43060 RD21B Cys E,V 0.99 (+) 1.49 (+ ) 0.59 1.36 SC AT5G10760...”
- Simultaneous expression of abiotic stress responsive transcription factors, AtDREB2A, AtHB7 and AtABF3 improves salinity and drought tolerance in peanut (Arachis hypogaea L.)
Pruthvi, PloS one 2014 - “...management of oxidative stress which resulted in reduced lipid peroxidation. Expression of AhProline amino peptidase (At4G30910; having ABRE ) and Drought Induced Protein ( DIP ; At4G15910 with ABRE and DRE ), was found to be more in transgenic plants than wild type indicating that the...”
- Evidence for the Existence in Arabidopsis thaliana of the Proteasome Proteolytic Pathway: ACTIVATION IN RESPONSE TO CADMIUM
Polge, The Journal of biological chemistry 2009 - “...At5g10540 At5g65620 At2g24200 At4g30920 At4g30910 At2g14260 At3g18780 At1g49240 AAGAAGGCGTTATCGAGGTG TCTACGGGTTCTTCGACCAG TGATCTACATCAAGGCTCTGTG...”
- “...two other hexameric LAPs exist, LAP2 (At4g30920) and LAP3 (At4g30910), both predicted to be chloroplastic. On the basis of PCR analysis, LAP1 is the most...”
Q10712 leucyl aminopeptidase (EC 3.4.11.1) from Solanum lycopersicum (see 4 papers)
35% identity, 80% coverage
P31427 Leucine aminopeptidase, chloroplastic from Solanum tuberosum
35% identity, 80% coverage
FTL_1108 cytosol aminopeptidase family protein from Francisella tularensis subsp. holarctica
34% identity, 68% coverage
- Diverse roles of low-molecular weight thiol GSH in <i>Francisella</i>'s virulence, location sensing and GSH-stealing from host
van, Current research in microbial sciences 2024 - “...pepA** [EC:3.4.11.1] (K01255) FTT_1318c FTN_0660 FTL_1479 cytosol leucyl Aminopeptidase B, pepB [EC:3.4.11.1] (K01255) FTT_1094c FTN_0780 FTL_1108 (DA46-1685) membrane alanyl Aminopeptidase N, pepN; [EC:3.4.11.2] (K01256) FTT_1793c FTN_1768 FTL_1956 Intra-bacterial Glutathione/GSH biosynthesis (cytoplasmic) ( Fig.1 B) Glutamate-CSH ligase, gshA # [EC:6.3.2.2] (K01919) FTT_0367c FTN_0277 FTL_1304 Glutathione synthetase, gshB...”
- “...Ramseyetal., 2020 ) Essential ( Ireland etal., 2019 ) pepB Amino peptidase B FTN_0780; FTT_1094c; FTL_1108 (DA461685). 1.5 No data No Change at 4/8h. Not essential ( Ramseyetal., 2020 ) Not essential ( Ramseyetal., 2020 ) No effect reported ( Ireland etal., 2019 ) pepN Amino...”
- Glutathione provides a source of cysteine essential for intracellular multiplication of Francisella tularensis
Alkhuder, PLoS pathogens 2009 - “...We identified the three orthologs of these aminopeptidases in the LVS genome (FTL_1479, FTL_1956, and FTL_1108, sharing 48%, 48% and 38% amino acid identity with PepA, B and N, respectively). It is thus reasonable to assume that LVS has the capacity to efficiently cleave cysteinylglycine peptides...”
- Hfq, a novel pleiotropic regulator of virulence-associated genes in Francisella tularensis
Meibom, Infection and immunity 2009 - “...FTL_1065 FTL_1066 FTL_1067 FTL_1068 FTL_1074 FTL_1106 FTL_1108 FTL_1174 FTL_1177 FTL_1190 FTL_1228 FTL_1229 FTL_1230 FTL_1261 FTL_1262 FTL_1266 FTL_1267...”
CBU_0572 cytosol aminopeptidase family protein from Coxiella burnetii RSA 493
37% identity, 69% coverage
- Proteomics paves the way for Q fever diagnostics
Kowalczewska, Genome medicine 2011 - “...of Cb , chronic Q fever markers 86 identified OMPs. SP: htpB, CBU_0236, CBU_0263, CBU_1471, CBU_0572, CBU_0235, CBU_1916, CBU_0612, CBU_0937 [ 56 ] Immunoproteomics or serology Candidate proteins for acute Q fever serodiagnosis IP/serodiagnosis 17 Cb strains (CC) (BGM or L929) RP ada (rada), AS immunized...”
- “...or vaccine development Phase-I-specific SP: CBU_1706, CBU_12190, CBU_0495, CBU_0780, CBU_0955, Phase-II-specific SP: CBU_1290, CBU_0235, CBU_0215, CBU_0572, CBU_1398, CBU_0236, CBU_0092, CBU_1241, CBU_1396, CBU_1227, CBU_0481, CBU_0299, CBU_0481, CBU_0495, CBU_0482, CBU_0937, CBU_0236 [ 68 ] RP-based approaches Candidate RPs for Q fever vaccine development and serodiagnosis TAP products and...”
SE0636 leucyl aminopeptidase from Staphylococcus epidermidis ATCC 12228
32% identity, 82% coverage
XP_626197, cgd5_2600 leucine aminopeptidase; of possible plant or bacterial origin from Cryptosporidium parvum Iowa II
34% identity, 77% coverage
- In Silico Identification and Functional Characterization of Conserved miRNAs in the Genome of Cryptosporidium parvum
Ahsan, Bioinformatics and biology insights 2021 - “...respectively. In addition, we found involvements of 5 genes named cgd8_3430, cgd5_4560, cgd8_4620, cgd3_80, and cgd5_2600 with some nutrient metabolic and biosynthesis pathways such as glutathione metabolism pathway; glycine, serine, and threonine metabolism pathway; purine metabolism pathway; cysteine and methionine metabolism pathway; and phenylalanine, tyrosine, and...”
- “...synthase) GSTMP; PTTBP cgd8_4620 (ISWI-related chromatinic protein) Purine metabolism cgd3_80 (S-adenosylhomocysteinase) Cysteine and methionine metabolism cgd5_2600 (leucine aminopeptidase) Glutathione metabolism cgd8_3430 (zincin/aminopeptidase Nlike metalloprotease) Glutathione metabolism Abbreviations: miRNA, microRNA; GSTMP, glycine, serine, and threonine metabolism pathway; PTTBP, phenylalanine, tyrosine, and tryptophan biosynthesis pathway. Discussion This study...”
- Molecular cloning and characterization of a M17 leucine aminopeptidase of Cryptosporidium parvum.
Kang, Parasitology 2011 (PubMed)- GeneRIF: sequence alignment of the deduced amino acid sequence of CpLAP with those of other organisms revealed that typical amino acid residues essential for metal binding and active-site formation in M17 LAPs were well conserved in CpLAP
- Determining the protein repertoire of Cryptosporidium parvum sporozoites
Sanderson, Proteomics 2008 - “...(lipase/esterase), cgd4_700 (hypothetical protein), cgd5_2600 (leucine aminopeptidase), cgd5_4560 (tryptophan synthase), cgd6_1910 (chagasin), cgd6_4570...”
- “...high MOWSE scores and good peptide coverage (e.g. cgd5_2600, leucine aminopeptidase; score 658, 15 peptides, 34% coverage) which is usually an indicator of high...”
A0A0S2K958 Leucyl aminopeptidase from Pseudoalteromonas phenolica
37% identity, 69% coverage
Q09735 leucyl aminopeptidase (EC 3.4.11.1) from Schizosaccharomyces pombe (see paper)
SPAC13A11.05 peptidase family M17 from Schizosaccharomyces pombe
37% identity, 61% coverage
MLC_1870 M17 family metallopeptidase from Mycoplasma mycoides subsp. capri LC str. 95010
42% identity, 61% coverage
- Proteases as Secreted Exoproteins in Mycoplasmas from Ruminant Lungs and Their Impact on Surface-Exposed Proteins
Ganter, Applied and environmental microbiology 2019 (secret) - Mycoplasma mycoides, from "mycoides Small Colony" to "capri". A microevolutionary perspective
Thiaucourt, BMC genomics 2011 - “...them was similar to a gene in MmmSC PG1 (a lipoprotein). Three Mmc 95010 genes (MLC_1870, 3270, 4830, coding for a leucylpeptidase, a protease and an endopeptidase, respectively) had orthologues in MmmSC PG1 that are altered: the MmmSC leucylpeptidase gene is truncated, giving two CDS (MSC_0234,...”
Krad_1149 leucyl aminopeptidase family protein from Kineococcus radiotolerans SRS30216 = ATCC BAA-149
37% identity, 69% coverage
Synpcc7942_1190 Leucyl aminopeptidase from Synechococcus elongatus PCC 7942
40% identity, 64% coverage
- An unexpected role for leucyl aminopeptidase in UV tolerance revealed by a genome-wide fitness assessment in a model cyanobacterium
Weiss, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...at 15 min and up-regulated at 2 h of UVR exposure. The leucyl aminopeptidase gene Synpcc7942_1190 is highlighted in section V . Assessment of Genome-Wide Fitness under UVR Using RB-TnSeq. A fresh culture of the RB-TnSeq mutant library of S. elongatus PCC 7942, recovered from a...”
- “...<0.05) ( Fig. 3 ). The top 5 mutant loci that strongly decreased fitness are Synpcc7942_1190 , Synpcc7942_0112 , Synpcc7942_1945 , Synpcc7942_1679 , and Synpcc7942_1616 . These genes are currently annotated as encoding a leucyl aminopeptidase (LAP), a deoxyribodipryrimidine photolyase, an excinuclease ABC subunit C, a...”
MCAP_0195 cytosol aminopeptidase from Mycoplasma capricolum subsp. capricolum ATCC 27343
41% identity, 59% coverage
- The Mycoplasma spp. 'Releasome': A New Concept for a Long-Known Phenomenon
Gaurivaud, Frontiers in microbiology 2022 - “...medium Exoproteome Voros et al., 2015 Cytosol aminopeptidase M. capricolum subsp. capricolum Ckid MCAP_0127 ; MCAP_0195 Serum reduced medium Exoproteome Voros et al., 2015 Aminopeptidase M. flocculare ATCC 27716 WP_002557776.1 Infected cells (serum free medium) Exoproteome Leal Zimmer et al., 2019 M. hyopneumoniae J AAZ44217.1 Infected...”
Q9VSM7 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
40% identity, 52% coverage
- Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera: identification of proteins binding the delta-endotoxin, Cry1Ac of Bacillus thuringiensis
Angelucci, Insect biochemistry and molecular biology 2008 - “...CG7340, AAF32329 ; HsPEL1, Q8NDH3 ; BmAPNL, Q8T105 ; CG32351 , Q9VSM7 ; HsAPN-L, AAH65564 ; Ae Q95PB9 , AAK55416 ; CG11951, Q9VAM2 ; CG14516 , Q8SYT3 ; CG5839 ,...”
Q9VSM6 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
40% identity, 52% coverage
MSC_0235 leucyl aminopeptidase from Mycoplasma mycoides subsp. mycoides SC str. PG1
41% identity, 60% coverage
Q8RLK7 bacterial leucyl aminopeptidase (EC 3.4.11.10) from Mycoplasmopsis gallinarum (see paper)
37% identity, 60% coverage
AMPLN_SOLLC / Q42876 Neutral leucine aminopeptidase, chloroplastic; LAP-N; Leucyl aminopeptidase N; Proline aminopeptidase N; Prolyl aminopeptidase N; EC 3.4.11.1; EC 3.4.11.5 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see 4 papers)
38% identity, 66% coverage
- function: Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (PubMed:12746529). When associated as homohexamer, catalyzes the proteolyzes of Xaa-Leu dipeptides (PubMed:12746529). Possesses leucine aminopeptidase activity against the model substrate leucine-amido methyl coumarin (PubMed:22493451). Presumably involved in the processing and regular turnover of intracellular proteins (Probable).
function: Functions as a molecular chaperone to protect proteins from heat-induced damage.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mn(2+) (Binds 2 Mn(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers). - Allergen false-detection using official bioinformatic algorithms.
Herman, GM crops & food 2020 - “...P46301 Q5ZEL0 Q5NE21 1 C7J0T2 1 K4B3I4 Q6ZHP6 1 Q9ZR41 P30298 O24024 Q8H8B0 2 1 Q42876 Q8H920 P38546 1 A3AHG5 5 Q6QLU0 Previous work using 50,090 protein sequences from maize found the sliding-window bioinformatic approach to falsely implicate 19.9% of putative non-allergens as allergens, while the...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...Homo sapiens (AAD17527); L.escu, Lycopersicon esculentum (Q42876); L.escuChl, L. esculentum Chloroplast (Q10712); L.pLAP, L. pneumophila LAP; M.lepr,...”
LAP-N / Q8GZD8 neutral leucine aminopeptidase (EC 3.4.11.9) from Solanum lycopersicum (see paper)
Q8GZD8 leucyl aminopeptidase (EC 3.4.11.1) from Solanum lycopersicum (see 2 papers)
NP_001233884 leucine aminopeptidase 2, chloroplastic from Solanum lycopersicum
39% identity, 65% coverage
Q9S2Q7 Probable cytosol aminopeptidase from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
SCO2179 aminopeptidase from Streptomyces coelicolor A3(2)
41% identity, 65% coverage
- The Streptomyces coelicolor Small ORF trpM Stimulates Growth and Morphological Development and Exerts Opposite Effects on Actinorhodin and Calcium-Dependent Antibiotic Production
Vassallo, Frontiers in microbiology 2020 - “...the UniProtKB database, the protein product of pepA is a putative cytosol aminopeptidase (entry identifier Q9S2Q7) belonging to the peptidase M17 family. In order to have an experimental confirmation of the PepA function, pepA was cloned in the expression vector pRSET-B and over-expressed in E. coli...”
- Editorial: Regulation of Antibiotic Production in Actinomycetes
Mast, Frontiers in microbiology 2020 - “...in Streptomyces coelicolor . TrpM is suggested to interact with the putative cytosol aminopeptidase PepA (SCO2179) via a post-transcriptional and/or post-translational regulatory mechanism, whereby PepA plays a key role in antibiotic production and sporulation. Overall, this collection of articles gives a profound overview on the importance...”
- The Streptomyces coelicolor Small ORF trpM Stimulates Growth and Morphological Development and Exerts Opposite Effects on Actinorhodin and Calcium-Dependent Antibiotic Production
Vassallo, Frontiers in microbiology 2020 - “...and morphological differentiation. Indeed, it was demonstrated that TrpM is able to interact with PepA (SCO2179), a putative cytosol aminopeptidase playing a key role in antibiotic production and sporulation. In this work, a S. coelicolor trpM knock-in (Sco- trpM KI) mutant strain was generated by cloning...”
- “...His-tagged TrpM allowed to identify the putative cytosol L-leucine-aminopeptidase PepA belonging to M17 metalloprotease family (SCO2179), the ribosomal protein S1 (SCO1998) and the ribosomal protein S2 (SCO5624) as probable interacting proteins of this SEP. TrpM and PepA interaction was also demonstrated by bacterial two-hybrid assay (...”
- Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in Streptomyces coelicolor
Xu, Applied and environmental microbiology 2019 - “...SCO4118 , SCO4215 , SCO4358 , and SCO5351 , and eight ACT downmodulators, SCO1728 , SCO2179 , SCO2686 , SCO3008 , SCO3579 ( wblA ), SCO3664 , SCO4426 ( afsR ), and SCO5803 ( lexA ); seven DNA transaction genes, SCO4127 to SCO4131 ( cmdBCDEF ),...”
- “...production by changing the expression of the biosynthetic gene clusters, e.g., atrA , nsdA , SCO2179 , and wblA ( 41 , 43 , 64 , 65 ), or activation of the substrate, e.g., SCO1596 ( 66 ). Other ACT-modulating genes did not fall into the...”
- TrpM, a Small Protein Modulating Tryptophan Biosynthesis and Morpho-Physiological Differentiation in Streptomyces coelicolor A3(2)
Palazzotto, PloS one 2016 - “...(PepA) in the prey protein fraction; they are the products of SCO1998 , SCO5624 and SCO2179 genes, respectively. Interestingly, PepA was shown to play a role in S . coelicolor A3(2) antibiotic production and sporulation, although its molecular function has not yet been demonstrated [ 21...”
- “...(possibly Trp dependent) roles for TrpM in controlling mRNA translation steps. 10.1371/journal.pone.0163422.g006 Fig 6 Protein SCO2179 (PepA) association network. (A) STRING interrogation showed PepA association with enzymes involved in amino acid metabolism: glycine hydroxymethyltransferase (GlyA); glutamate-cysteine ligase (GshA); cysteine synthases (CysK and CysM); glutathione hydrolase (GGT)....”
- Deletion of an architectural unit, leucyl aminopeptidase (SCO2179), in Streptomyces coelicolor increases actinorhodin production and sporulation
Song, Applied microbiology and biotechnology 2013 (PubMed)- “...of an architectural unit, leucyl aminopeptidase (SCO2179), in Streptomyces coelicolor increases actinorhodin production and sporulation Eunjung Song &...”
- “...protein did not exhibit leucyl aminopeptidase activity. SCO2179 deletion mutant showed increased actinorhodin production and sporulation, as well as more...”
AMPL2_SOLLC / O24022 Leucine aminopeptidase A2, chloroplastic; LapA2; Lap17.1a protein; Leucyl aminopeptidase 2; LAP 2; Proline aminopeptidase 2; Prolyl aminopeptidase 2; EC 3.4.11.1; EC 3.4.11.5 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum) (see 4 papers)
35% identity, 80% coverage
- function: Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity). When associated as homohexamer, catalyzes the proteolyzes of Xaa-Leu dipeptides (By similarity). Possesses leucine aminopeptidase activity against the model substrate leucine-amido methyl coumarin (By similarity). Presumably involved in the processing and regular turnover of intracellular proteins (Probable). Regulates wound signaling and has a role in insect defense (By similarity).
function: Functions as a molecular chaperone to protect proteins from heat-induced damage.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
catalytic activity: Release of N-terminal proline from a peptide.
cofactor: Mg(2+) (Binds 2 Mg(2+) ions per subunit.)
subunit: Homohexamer (dimer of homotrimers).
NP_001328632 Cytosol aminopeptidase family protein from Arabidopsis thaliana
34% identity, 64% coverage
XP_974656 cytosol aminopeptidase from Tribolium castaneum
D6WAL5 Cytosol aminopeptidase from Tribolium castaneum
38% identity, 54% coverage
C0P2V1 Leucine aminopeptidase 2 chloroplastic from Zea mays
46% identity, 54% coverage
- A comparative glycoproteome study of developing endosperm in the hexose-deficient miniature1 (mn1) seed mutant and its wild type Mn1 in maize
Silva-Sanchez, Frontiers in plant science 2014 - “...14 8 1 0.13 B6T7Q7 Serine hydroxymethyltransferase 8.6 56.53 6 9 2 0.02 M 403 C0P2V1 Simillar to Leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 5.22 5.62 61.20 28.37 1.48 0.76 5 4 3 0.13 Matsushita-Morita et al., 2011 B4F8W6 Simillar to UTPglucose-1-phosphate uridylyltransferase from...”
- Proteomic comparison of basal endosperm in maize miniature1 mutant and its wild-type Mn1
Silva-Sanchez, Frontiers in plant science 2013 - “...division cycle protein 48 (Fragment) 0.96 0.04 1.23 0.03 B6TNF1 Calnexin 0.98 0.08 1.24 0.01 C0P2V1 Similar to leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 0.98 0.05 1.35 0.03 C0HGV5 Enolase 1.01 0.02 1.46 0.08 Q5EUD6 b Protein disulfide isomerase 1.02 0.01 1.32 0.04 B7ZWY9...”
- “...Putative uncharacterized protein 1.21 0.02 1.13 0.02 B6SPX4 Tubulin alpha-3 chain 1.22 0.02 1.15 0.03 C0P2V1 Similar to leucine aminopeptidase 2, chloroplastic from Oryza sativa (Q6K669) 1.23 0.02 1.03 0.06 B4FTP4 Putative uncharacterized protein 1.26 0.01 1.65 0.05 C0PDB6 Similar to 3-N-debenzoyl-2-deoxytaxol N-benzoyltransferase from Zea mays...”
A0A336UEJ6 leucyl aminopeptidase (EC 3.4.11.1) from Echinococcus granulosus (see paper)
31% identity, 58% coverage
Q500X4 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
35% identity, 54% coverage
Krad_3276 leucyl aminopeptidase from Kineococcus radiotolerans SRS30216 = ATCC BAA-149
36% identity, 73% coverage
mhp462 aminopeptidase from Mycoplasma hyopneumoniae 232
36% identity, 64% coverage
- Development of an indirect ELISA for detection of anti-Mycoplasma hyopneumoniae IgG in naturally infected pathogen-induced convalescent sera
Tian, BMC veterinary research 2021 - “...13 ]. The studies identified 15 serological immunodominant proteins and 1 discriminative serological immunodominant protein, Mhp462 [ 14 ]. These data suggest that a Mhp366-based ELISA has potential to be used as a diagnostic tool to detect natural M. hyopneumoniae infection. The objective of this study...”
- “...Wen Y Xu Z Tian Y Yang M Wang X Yang Y Ding H Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae Heliyon 2020 6 e04832 10.1016/j.heliyon.2020.e04832 32923730 15. Holst S Yeske P Pieters M Elimination of Mycoplasma hyopneumoniae...”
- Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae
Ning, Heliyon 2020 - “...Heliyon Heliyon Heliyon 2405-8440 Elsevier 7476235 S2405-8440(20)31675-3 10.1016/j.heliyon.2020.e04832 e04832 Article Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae Ning Yaru a 1 Zhou Yaoqin b 1 Wang Zhaodi a 1 Wen Yukang a Xu Zuobo...”
- “...as serological immunodominant antigens, while 3 proteins were not recognized by any convalescent serum. Moreover, Mhp462, a leucine aminopeptidase, was found to be a discriminative serological immunodominant antigen which reacted with convalescent sera but not with hyperimmune sera. The serological immunodominant proteins were antigenic and were...”
- Pathogenicity & virulence of Mycoplasma hyopneumoniae
Leal, Virulence 2020 - “...] L-lactate dehydrogenase (LDH) mhp245 MHP7448_0137 MHJ_0133 Fibronectin, heparin Y [ 78 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Plasminogen, heparin Y [ 10 ] Lipoprotein mhp390 MHP7448_0378 MHJ_0374 Porcine epithelial cilia NR [ 139 ] Lppt protein mhp384 MHP7448_0372 MHJ_0368 Heparin, porcine epithelial cilia Y [...”
- “...] Hemolysin C mhp663 MHP7448_0643 MHJ_0643 Cytotoxicity Su [ 75 , 174 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Proteolytic processing, adhesion Su [ 10 , 75 ] Lipoprotein mhp164 MHP7448_0217 MHJ_0213 Cytotoxicity Se/Su [ 21 , 26 , 75 , 138 ] Lipoprotein mhp502 MHP7448_0505 MHJ_0502...”
DR_0717 leucyl aminopeptidase, putative from Deinococcus radiodurans R1
39% identity, 72% coverage
MHP7448_0464 leucyl aminopeptidase from Mycoplasma hyopneumoniae 7448
36% identity, 64% coverage
- Pathogenicity & virulence of Mycoplasma hyopneumoniae
Leal, Virulence 2020 - “...L-lactate dehydrogenase (LDH) mhp245 MHP7448_0137 MHJ_0133 Fibronectin, heparin Y [ 78 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Plasminogen, heparin Y [ 10 ] Lipoprotein mhp390 MHP7448_0378 MHJ_0374 Porcine epithelial cilia NR [ 139 ] Lppt protein mhp384 MHP7448_0372 MHJ_0368 Heparin, porcine epithelial cilia Y [ 78...”
- “...Hemolysin C mhp663 MHP7448_0643 MHJ_0643 Cytotoxicity Su [ 75 , 174 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Proteolytic processing, adhesion Su [ 10 , 75 ] Lipoprotein mhp164 MHP7448_0217 MHJ_0213 Cytotoxicity Se/Su [ 21 , 26 , 75 , 138 ] Lipoprotein mhp502 MHP7448_0505 MHJ_0502 Cytotoxicity...”
- Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae
Ning, Heliyon 2020 - “...MHP7448_0368 MHJ_0364 MHP168_393 MHL_3046 Mhp390 MHP7448_0378 MHJ_0274 MHP168_418 MHL_2982 Mhp424 MHP7448_0408 MHJ_0421 MHP168_433 MHL_2950 Mhp462 MHP7448_0464 MHJ_0461 MHP168_474 MHL_3251 Mhp465 MHP7448_0467 MHJ_0464 MHP168_477 MHL_2914 Mhp472 MHP7448_0474 MHJ_0471 MHP168_484 MHL_3246 Mhp477 MHP7448_0479 MHJ_0476 MHP168_489 MHL_1747 Mhp483 MHP7448_0485 MHJ_0482 MHP168_495 MHL_3418 Mhp488 MHP7448_0490 MHJ_0487 MHP168_500 MHL_1789 Mhp504 MHP7448_0507...”
- Comparative proteomics of two Mycoplasma hyopneumoniae strains and Mycoplasma flocculare identified potential porcine enzootic pneumonia determinants
Paes, Virulence 2018 - “...[ 72 ] MHP7448_0263 Energy-coupling factor transporter ATP-binding protein EcfA1 - 5.76 [ 73 ] MHP7448_0464 Leucyl aminopeptidase - - 2.09 3.95 [ 10 ] MHP7448_0133 Lipase-esterase - 13.40 - - [ 74 ] MHP7448_0137 L-lactate dehydrogenase - 62.92 - - [ 36 ] MHP7448_0524 Lon...”
- “...SuPE, the oligoendopeptidase F and leucyl aminopeptidase were overrepresented in the pathogenic mycoplasma (MHP7448_0521, and MHP7448_0464, respectively). In comparison to M. flocculare , 6 proteases were overrepresented in M. hyopneumoniae 7448 samples, as follows. An aminopeptidase (MHP7448_0129) and the methionine aminopeptidase (MHP7448_0173) were more abundant in...”
Q4A9M4 leucyl aminopeptidase (EC 3.4.11.1) from Mycoplasma hyopneumoniae (see paper)
36% identity, 64% coverage
- Intracellular proteins moonlighting as bacterial adhesion factors.
Jeffery, AIMS microbiology 2018 - “...mucus [42] Hsp65/Cpn60.2/GroEL2 Mycobacterium tuberculosis A0A0H3LCC3 CD43 on macrophage surface [92] Leucyl aminopeptidase Mycoplasma hyopneumoniae Q4A9M4 heparin [93] Malate synthase Mycobacterium tuberculosis P9WK17 fibronectin, laminin, epithelial cells [38] Glutamyl aminopeptidase Mycoplasma hyopneumoniae Q4AAK4 plasminogen, heparin [47] Leucyl aminopeptidase Mycoplasma hyopneumoniae Q4A9M4 plasminogen, heparin, DNA [48] Ornithine...”
- Post-translational processing targets functionally diverse proteins in Mycoplasma hyopneumoniae.
Tacchi, Open biology 2016 (no snippet) - MHJ_0125 is an M42 glutamyl aminopeptidase that moonlights as a multifunctional adhesin on the surface of Mycoplasma hyopneumoniae.
Robinson, Open biology 2013 - “...), subtilisin-like serine protease (MHJ_0085, Q4AAP2 ), methionine aminopeptidase (MHJ_0169, Q4AAG1 ), leucyl aminopeptidase (MHJ_0461, Q4A9M4 ), oligoendopeptidase F (MHJ_0522, Q4A9G3 ) and Xaa-Pro aminopeptidase (MHJ_0659, Q4A929 ), [ 22 24 ]. Our proteome studies, performed on strain J, indicate that eight putative proteases are expressed...”
MHJ_0461 leucyl aminopeptidase from Mycoplasma hyopneumoniae J
36% identity, 64% coverage
- Research Progress on Immune Evasion of Mycoplasma hyopneumoniae
Jiang, Microorganisms 2024 - “...]. Some adhesins can also act as virulence factors for Mhp in pathogenesis, such as MHJ_0461 [ 63 ] and P116 [ 36 ]. Adhesins often undergo proteolytical processing [ 64 ], which produces different protein forms with their own adhesion properties. Adhesins are proteolytically hydrolyzed...”
- “...V.M. Santos J. Tacchi J.L. Raymond B.B. Deutscher A.T. Jenkins C. Padula M.P. Djordjevic S.P. MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae Open Biol. 2015 5 140175 10.1098/rsob.140175 25589579 64. Machado L. Paes J.A. Souza Dos Santos P. Ferreira H.B. Evidences...”
- Host-gut microbiota interactions shape parasite infections in farmed Atlantic salmon
Brealey, mSystems 2024 - “...mobile had glycerol-3-phosphate oxidase, which generates cytotoxic hydrogen peroxide through glycerol metabolism. One putative adhesin (MHJ_0461) was conserved across Mycoplasma genomes. The M. mobile clade genomes also contained additional genes reported to be involved in adherence ( 41 ). Focusing on other virulence factors, the MAG02/MAG04...”
- Infection strategies of mycoplasmas: Unraveling the panoply of virulence factors
Yiwen, Virulence 2021 - “...+ - - [ 50 ] MHJ_0125 ~40 - - + + [ 51 ] MHJ_0461 51.4 - - + + [ 52 ] M. bovis P27 27 - + - - [ 69 ] Eno 49 - - + - [ 70 ] TrmFO 48.8...”
- “...including invasion and persistent infections [ 50 ]. Moreover, both MHJ_0125, a glutamyl aminopeptidase, and MHJ_0461, a leucine aminopeptidase, have moonlighting roles as adhesins on the surface of M. hyopneumoniae ; MHJ_0461 also binds to foreign double-stranded DNA [ 51 , 52 ]. Eno in M....”
- Elevated Mhp462 antibody induced by natural infection but not in vitro culture of Mycoplasma hyopneumoniae
Ning, Heliyon 2020 - “...MHJ_0364 MHP168_393 MHL_3046 Mhp390 MHP7448_0378 MHJ_0274 MHP168_418 MHL_2982 Mhp424 MHP7448_0408 MHJ_0421 MHP168_433 MHL_2950 Mhp462 MHP7448_0464 MHJ_0461 MHP168_474 MHL_3251 Mhp465 MHP7448_0467 MHJ_0464 MHP168_477 MHL_2914 Mhp472 MHP7448_0474 MHJ_0471 MHP168_484 MHL_3246 Mhp477 MHP7448_0479 MHJ_0476 MHP168_489 MHL_1747 Mhp483 MHP7448_0485 MHJ_0482 MHP168_495 MHL_3418 Mhp488 MHP7448_0490 MHJ_0487 MHP168_500 MHL_1789 Mhp504 MHP7448_0507 MHJ_0504...”
- “...with inactivated vaccine failed to induce IgG antibody against Mhp366 [ 37 ]. Mhp462, named MHJ_0461 in J strain, forms multimers comprising more than 10 subunits on the surface M.hyopneumoniae [ 32 ]. Two main complexes are approximately 500 kDa and 800 kDa naturally, although the...”
- Genomic Variability and Post-translational Protein Processing Enhance the Immune Evasion of Mycoplasma hyopneumoniae and Its Interaction With the Porcine Immune System
Li, Frontiers in immunology 2020 - “...), P116 ( 44 ), glutamyl aminopeptidase (GAP; MHJ_0125) ( 47 ), leucine aminopeptidase (LAP; MHJ_0461) ( 54 ), and fructose-1, 6-bisphosphate aldolase (FBA) ( 55 ). The plasminogen activation can improve the Mhp adhesion efficiency and stimulate cytokines release like tumor necrosis factor (TNF-), interleukin...”
- “...21951786 54. Jarocki VM Santos J Tacchi JL Raymond BB Deutscher AT Jenkins C . MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae . Open Biol . ( 2015 ) 5 : 140175 87 . 10.1098/rsob.140175 25589579 55. Yu Y Liu M...”
- Pathogenicity & virulence of Mycoplasma hyopneumoniae
Leal, Virulence 2020 - “...dehydrogenase (LDH) mhp245 MHP7448_0137 MHJ_0133 Fibronectin, heparin Y [ 78 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Plasminogen, heparin Y [ 10 ] Lipoprotein mhp390 MHP7448_0378 MHJ_0374 Porcine epithelial cilia NR [ 139 ] Lppt protein mhp384 MHP7448_0372 MHJ_0368 Heparin, porcine epithelial cilia Y [ 78 ,...”
- “...C mhp663 MHP7448_0643 MHJ_0643 Cytotoxicity Su [ 75 , 174 ] Leucyl aminopeptidase mhp462 MHP7448_0464 MHJ_0461 Proteolytic processing, adhesion Su [ 10 , 75 ] Lipoprotein mhp164 MHP7448_0217 MHJ_0213 Cytotoxicity Se/Su [ 21 , 26 , 75 , 138 ] Lipoprotein mhp502 MHP7448_0505 MHJ_0502 Cytotoxicity Se/Su...”
- Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae
Chen, Frontiers in cellular and infection microbiology 2019 - “...M. hyopneumoniae (Tacchi et al., 2016 ). P159 (Raymond et al., 2013 ), amino peptidases MHJ_0461 and MHJ_0125 (Robinson et al., 2013 ; Jarocki et al., 2015 ), EF-Tu (Yu et al., 2018a ), and some other moonlighting proteins are newly determined adhesins. The genomics and...”
- “...well as large numbers of moonlighting proteins, such as MHJ_0125 (Robinson et al., 2013 ), MHJ_0461 (Jarocki et al., 2015 ), EF-Tu (Yu et al., 2018a ), FBA (Yu et al., 2018b ), Mhp Eno. Furthermore, the number of adhesins is enlarged by posttranslation processing (Deutscher...”
- Fructose-1,6-bisphosphate aldolase encoded by a core gene of Mycoplasma hyopneumoniae contributes to host cell adhesion
Yu, Veterinary research 2018 - “...aminopeptidase that moonlights as an adhesin on the surface of M. hyopneumoniae [ 15 ]; MHJ_0461, a leucine aminopeptidase which binds to heparin, plasminogen and foreign DNA and functions as an accessory adhesin [ 16 ]; and l -lactate dehydrogenase, an immunogenic cytoplasmic protein involved in...”
- “...VM Santos J Tacchi JL Raymond BB Deutscher AT Jenkins C Padula MP Djordjevic SP MHJ_0461 is a multifunctional leucine aminopeptidase on the surface of Mycoplasma hyopneumoniae Open Biol 2015 5 140175 10.1098/rsob.140175 25589579 17. Tacchi JL Raymond BB Haynes PA Berry IJ Widjaja M Bogema...”
- More
Q95R35 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
35% identity, 54% coverage
D6WIH3 Cytosol aminopeptidase from Tribolium castaneum
XP_972664 cytosol aminopeptidase from Tribolium castaneum
31% identity, 76% coverage
PMCN06_0200 aminopeptidase PepB from Pasteurella multocida subsp. multocida str. HN06
36% identity, 63% coverage
- Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates
Du, International journal of genomics 2016 - “...transposase PmCQ2_4g0359 IS200 transposase PmCQ2_2g0080 Pmu_00570 PMCN06_0130 NT08PM_0122 PM1098 Glycyl-tRNA synthetase subunit alpha PmCQ2_2g0148 Pmu_01310 PMCN06_0200 NT08PM_0197 PM1029 Peptidase B PmCQ2_2g0349 Pmu_04490 PMCN06_0404 NT08PM_0930 PM0385 Electron transport complex protein RnfC PmCQ2_2g0231 Pmu_15590 NT08PM_1618 PM1778 Hypothetical protein PmCQ2_6g0020 Hypothetical protein PmCQ2_2g0066 Transposase IS605 PmCQ2_2g0097 Transposase IS605 PmCQ2_6g0026...”
PM1029 PepB from Pasteurella multocida subsp. multocida str. Pm70
36% identity, 63% coverage
- Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates
Du, International journal of genomics 2016 - “...IS200 transposase PmCQ2_2g0080 Pmu_00570 PMCN06_0130 NT08PM_0122 PM1098 Glycyl-tRNA synthetase subunit alpha PmCQ2_2g0148 Pmu_01310 PMCN06_0200 NT08PM_0197 PM1029 Peptidase B PmCQ2_2g0349 Pmu_04490 PMCN06_0404 NT08PM_0930 PM0385 Electron transport complex protein RnfC PmCQ2_2g0231 Pmu_15590 NT08PM_1618 PM1778 Hypothetical protein PmCQ2_6g0020 Hypothetical protein PmCQ2_2g0066 Transposase IS605 PmCQ2_2g0097 Transposase IS605 PmCQ2_6g0026 Transposase IS605...”
D2W4E3 Leucine aminopeptidase from Naegleria gruberi
38% identity, 51% coverage
E1Z575 PDZ domain-containing protein from Chlorella variabilis
CHLNCDRAFT_33905 hypothetical protein from Chlorella variabilis
38% identity, 63% coverage
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae
Rai, Scientific reports 2017 - “...0.9870.20 1.4780.38 1.7410.28 11. A0A087SEY5 Proteasome subunit alpha type-4 F751_5131 1 1.0350.24 1.6930.13 2.5660.12 12. E1Z575 Putative uncharacterized protein (Cytosol aminopeptidase) CHLNCDRAFT_33905 1 1.4930.60 1.9560.50 2.0470.19 13. E1ZMK0 Putative uncharacterized protein (metallo peptidase) CHLNCDRAFT_137495 1 0.3350.07 0.8350.29 2.1491.37 14. E1ZN67 Proteasome subunit alpha type CHLNCDRAFT_26444 3...”
- “...role in nitrogen-starvation induced lipid accumulation in microalgae References 1 Protein degradation 8 E1ZN67, E1ZQQ9, E1Z575, E1ZMK0, E1Z5I7, E1ZCR2, A0A087SEY5, E1ZR38 Enhanced protein degradation is required to maintain the intracellular nitrogen homeostasis. 6 , 49 , 50 2 Amino acid biosynthesis 9 E1ZPZ3, E1Z4T9, E1ZF33, A0A087SJX6,...”
- Real-time iTRAQ-based proteome profiling revealed the central metabolism involved in nitrogen starvation induced lipid accumulation in microalgae
Rai, Scientific reports 2017 - “...subunit alpha type-4 F751_5131 1 1.0350.24 1.6930.13 2.5660.12 12. E1Z575 Putative uncharacterized protein (Cytosol aminopeptidase) CHLNCDRAFT_33905 1 1.4930.60 1.9560.50 2.0470.19 13. E1ZMK0 Putative uncharacterized protein (metallo peptidase) CHLNCDRAFT_137495 1 0.3350.07 0.8350.29 2.1491.37 14. E1ZN67 Proteasome subunit alpha type CHLNCDRAFT_26444 3 1.5320.02 1.6660.14 2.5990.33 15. E1ZQQ9 Proteasome...”
NT08PM_0197, Pmu_01310 aminopeptidase PepB from Pasteurella multocida 36950
36% identity, 63% coverage
- Comparative Genomics Analysis of Two Different Virulent Bovine Pasteurella multocida Isolates
Du, International journal of genomics 2016 - “...PmCQ2_4g0359 IS200 transposase PmCQ2_2g0080 Pmu_00570 PMCN06_0130 NT08PM_0122 PM1098 Glycyl-tRNA synthetase subunit alpha PmCQ2_2g0148 Pmu_01310 PMCN06_0200 NT08PM_0197 PM1029 Peptidase B PmCQ2_2g0349 Pmu_04490 PMCN06_0404 NT08PM_0930 PM0385 Electron transport complex protein RnfC PmCQ2_2g0231 Pmu_15590 NT08PM_1618 PM1778 Hypothetical protein PmCQ2_6g0020 Hypothetical protein PmCQ2_2g0066 Transposase IS605 PmCQ2_2g0097 Transposase IS605 PmCQ2_6g0026 Transposase...”
- “...IS200 transposase PmCQ2_4g0359 IS200 transposase PmCQ2_2g0080 Pmu_00570 PMCN06_0130 NT08PM_0122 PM1098 Glycyl-tRNA synthetase subunit alpha PmCQ2_2g0148 Pmu_01310 PMCN06_0200 NT08PM_0197 PM1029 Peptidase B PmCQ2_2g0349 Pmu_04490 PMCN06_0404 NT08PM_0930 PM0385 Electron transport complex protein RnfC PmCQ2_2g0231 Pmu_15590 NT08PM_1618 PM1778 Hypothetical protein PmCQ2_6g0020 Hypothetical protein PmCQ2_2g0066 Transposase IS605 PmCQ2_2g0097 Transposase IS605...”
YfhI / b2523 aminopeptidase B (EC 3.4.11.23; EC 3.4.13.18) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
pepB / P37095 aminopeptidase B (EC 3.4.11.23; EC 3.4.13.18) from Escherichia coli (strain K12) (see 6 papers)
PEPB_ECOLI / P37095 Peptidase B; Aminopeptidase B; EC 3.4.11.23 from Escherichia coli (strain K12) (see paper)
pepB / RF|NP_417018 peptidase B from Escherichia coli K12 (see 2 papers)
b2523 putative peptidase from Escherichia coli str. K-12 substr. MG1655
NP_417018 aminopeptidase B from Escherichia coli str. K-12 substr. MG1655
37% identity, 63% coverage
- function: Probably plays an important role in intracellular peptide degradation (PubMed:20067529).
catalytic activity: Release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln.
cofactor: Mn(2+) (Binds 2 manganese ions per subunit.)
subunit: Homohexamer.
disruption phenotype: A quadruple peptidase disruption (pepA, pepB, pepD and pepN) does not grow in M9 minimal medium, grows better when supplemented with casamino acids (PubMed:20067529). - Genetic determinants for n-butanol tolerance in evolved Escherichia coli mutants: cross adaptation and antagonistic pleiotropy between n-butanol and other stressors
Reyes, Applied and environmental microbiology 2013 - “...sodB gcvH hyfD nuoI pepB treF ygfO seta mdtA pgsA b1656 b2904 b2484 b2281 b2523 b3519 b2882 b0070 b2074 b1912 24 2 23 2 25 2 22 1 40 3 37 3 35 2 24 3 11 1 15 1...”
- Towards a rigorous network of protein-protein interactions of the model sulfate reducer Desulfovibrio vulgaris Hildenborough
Chhabra, PloS one 2011 - “...(b0014) included GrpE (b2614), chaperone protein HscA (b2526), ATP-dependent protease Lon (b0439), and Peptidase B (b2523) but not the chaperones DnaJ (b0015) and GroEL (b4143) [10] . However, in other studies in E. coli K12, DnaK, GrpE, and DnaJ have been demonstrated to form a chaperone...”
- Effect of RyhB small RNA on global iron use in Escherichia coli
Massé, Journal of bacteriology 2005 - “...b2279 b2280 b2281 b2282 b2283 b2284 b2285 b2286 b2287 b2288 b2522 b2523 b2524 b2525 b2526 4.4 2.1 6.1 6.1 4.0 5.1 1.9 1.7 3.8 1.7 2.9 7.6 3.0 19.3 2.0 2.8 4.7...”
- Sulfur and nitrogen limitation in Escherichia coli K-12: specific homeostatic responses
Gyaneshwar, Journal of bacteriology 2005 - “...also includes an additional five genes with related functions (b2523 to b2527) in this operon, but Ecocyc does not. We have no indication that these genes are...”
- Genome-wide transcriptional profiling of the Escherichia coli responses to superoxide stress and sodium salicylate
Pomposiello, Journal of bacteriology 2001 - “...b3312 b3314 b3321 b3316 b0724 b3908 b4062 b0729 b3708 b0850 b2523 b3520 b4217 b1852 acrA ahpC aldA artI artP b0710 b1378 b1452 b2351 b2962 cadA cadC ccmD cyoD...”
- Comparison of metal-bound and unbound structures of aminopeptidase B proteins from Escherichia coli and Yersinia pestis.
Minasov, Protein science : a publication of the Protein Society 2020 - GeneRIF: Comparison of metal-bound and unbound structures of aminopeptidase B proteins from Escherichia coli and Yersinia pestis.
- Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C.
Kazakov, Journal of bacteriology 2008 - GeneRIF: Data show that PepB can effectively process heptapeptide-nucleotide microcin C (McC).
- THE-DB: a threading model database for comparative protein structure analysis of the E. coli K12 and human proteomes
Diamond, Database : the journal of biological databases and curation 2018 - “...are exclusively found in THE-DB. The sequence with the UniProt identification ID of P15029, P23876, P37095, Q7Z4T9, Q9BT92 * and Q96M91 * is just a small fraction of cases that satisfy the above criteria ( * containing quality templates in THE-DB and contains no existing template...”
- Energetics-based protein profiling on a proteomic scale: identification of proteins resistant to proteolysis
Park, Journal of molecular biology 2007 - “...pckA 540 59.6 5.46 9 a P19926 agp 391 43.6 5.38 10 P37095 pepB 427 46.2 5.60 11 a P75691 yahK 349 38.0 5.80 12 P00509 aspC 396 43.6 5.54 13 P11665 Pgk 386 41.0...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...PdxY P77150 Pyridoxamine kinase 6.04/31,322.2 PepB P37095 Peptidase B 5.6/46,180.17 PepD P15288 Aminoacyl-histidine dipeptidase 5.20/52,784.22 5.22/52,046 (DIGE...”
- Salmonella enterica serovar typhimurium peptidase B is a leucyl aminopeptidase with specificity for acidic amino acids
Mathew, Journal of bacteriology 2000 - “...PepB (AF201078 [this work]); E.cPepB, E. coli PepB (P37095); Y.pPepB, Y. pestis PepB (SSC); S.pLAP3, S. putrefaciens LAP3 (NUMG); C.cLAP3, C. crescentus LAP3...”
- A genomic sample sequence of the entomopathogenic bacterium Photorhabdus luminescens W14: potential implications for virulence
Ffrench-Constant, Applied and environmental microbiology 2000 - “...Pea Limulus D78508 AF034088 P07000 U83995 P76403 A43329 P24555 P08395 P37095 AE000321 P37764 AE001618 AF062392 P08688 A40774 2 (0) 1 (0) 2 (0) 2 (0) 2 (0) 1 (0)...”
6ov8A / P37095 2.6 angstrom resolution crystal structure of aminopeptidase b from escherichia coli str. K-12 substr. Mg1655 (see paper)
37% identity, 63% coverage
- Ligands: zinc ion; manganese (ii) ion (6ov8A)
Q9KTX5 Peptidase B from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
VC0755 aminopeptidase B from Vibrio cholerae O1 biovar eltor str. N16961
32% identity, 72% coverage
Q9RF52 leucyl aminopeptidase (EC 3.4.11.1) from Salmonella enterica (see paper)
35% identity, 66% coverage
Q7K2S9 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
NP_001097338 loopin-1, isoform C from Drosophila melanogaster
35% identity, 54% coverage
NTHI1038 aminopeptidase B from Haemophilus influenzae 86-028NP
34% identity, 65% coverage
5lhkA / K4MHW2 Bottromycin maturation enzyme botp in complex with mn (see paper)
37% identity, 64% coverage
- Ligands: manganese (ii) ion; bicarbonate ion (5lhkA)
5ntgA / V5BWE3 Structure of leucyl aminopeptidase from trypanosoma cruzi in complex with citrate (see paper)
34% identity, 63% coverage
- Ligand: manganese (ii) ion (5ntgA)
Q4DZJ3 leucyl aminopeptidase (EC 3.4.11.1) from Trypanosoma cruzi (see paper)
36% identity, 60% coverage
Q8T105 Cytosol aminopeptidase from Bombyx mori
32% identity, 52% coverage
- Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera: identification of proteins binding the delta-endotoxin, Cry1Ac of Bacillus thuringiensis
Angelucci, Insect biochemistry and molecular biology 2008 - “..., see text; CG7340, AAF32329 ; HsPEL1, Q8NDH3 ; BmAPNL, Q8T105 ; CG32351 , Q9VSM7 ; HsAPN-L, AAH65564 ; Ae Q95PB9 , AAK55416 ; CG11951, Q9VAM2 ; CG14516 ,...”
Q6NSR8 Probable aminopeptidase NPEPL1 from Mus musculus
36% identity, 59% coverage
- Proteomic Analysis Identifies Multiple Mechanisms of 5-Fluorouracil-Induced Gut Mucositis in Mice.
Ivanov, Cancers 2024 - “...meprin A subunit Q61847 Mep1b 2.5 Proteolysis Kallikrein-1 P15947 Klk1 2.4 Proteolysis Probable aminopeptidase NPEPL1 Q6NSR8 Npepl1 2.1 Proteolysis Prolyl endopeptidase Q9QUR6 Prep 4.8 Proteolysis (digestive enzyme) Chymotrypsin-like elastase family member 2A P05208 Cela2a 4.7 Proteolysis (digestive enzyme) Chymotrypsin-like elastase family member 3B Q9CQ52 Cela3b 3.8...”
- Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.
Dabin, Oxidative medicine and cellular longevity 2022 - “...activity (GO:0016787~hydrolase activity) was the first pathway, including 16 related proteins (Q9CYC6, Q9CR30, Q50L41, Q9R001, Q6NSR8, Q8CGB6, Q9WUZ9, Q9ET22, Q91ZX6, P35821, P52479, Q8CHE4, Q9CXY9, P97470, P07146, Q14BV6). 3.5. DCP2 and Related Pathway Changes after TBI DCP2 (Q9CYC6) was found to increase in the TBI group by...”
- The identification of potential factors associated with the development of type 2 diabetes: a quantitative proteomics approach.
Lu, Molecular & cellular proteomics : MCP 2008
Q8T4F1 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
31% identity, 66% coverage
SPO3717 leucyl aminopeptidase family protein from Ruegeria pomeroyi DSS-3
33% identity, 84% coverage
- An Updated genome annotation for the model marine bacterium Ruegeria pomeroyi DSS-3
Rivers, Standards in genomic sciences 2014 - “...ORF position YP_168735 SPO3540 Hypothetical protein ORF position YP_168802 SPO3607 Hypothetical protein ORF position YP_168911 SPO3717 Cytosol aminopeptidase family protein ORF position YP_168940 SPO3746 Adenine deaminse ade ORF position YP_169017 SPO3829 S-formylglutathione hydrolase, putative ORF position YP_169021 SPO3833 ATP-dependent RNA helicase, DEAD/DEAH box family ORF position...”
SPO3717, YP_168911 cytosol aminopeptidase family protein from Silicibacter pomeroyi DSS-3
33% identity, 84% coverage
- An Updated genome annotation for the model marine bacterium Ruegeria pomeroyi DSS-3
Rivers, Standards in genomic sciences 2014 - “...ORF position YP_168735 SPO3540 Hypothetical protein ORF position YP_168802 SPO3607 Hypothetical protein ORF position YP_168911 SPO3717 Cytosol aminopeptidase family protein ORF position YP_168940 SPO3746 Adenine deaminse ade ORF position YP_169017 SPO3829 S-formylglutathione hydrolase, putative ORF position YP_169021 SPO3833 ATP-dependent RNA helicase, DEAD/DEAH box family ORF position...”
- “...protein ORF position YP_168735 SPO3540 Hypothetical protein ORF position YP_168802 SPO3607 Hypothetical protein ORF position YP_168911 SPO3717 Cytosol aminopeptidase family protein ORF position YP_168940 SPO3746 Adenine deaminse ade ORF position YP_169017 SPO3829 S-formylglutathione hydrolase, putative ORF position YP_169021 SPO3833 ATP-dependent RNA helicase, DEAD/DEAH box family ORF...”
PEPL1_HUMAN / Q8NDH3 Probable aminopeptidase NPEPL1; Aminopeptidase-like 1; EC 3.4.11.- from Homo sapiens (Human) (see paper)
36% identity, 59% coverage
- function: Probably catalyzes the removal of unsubstituted N-terminal amino acids from various peptides
cofactor: Zn(2+) Mn(2+) - Identification of Candidate Protein Markers in Skeletal Muscle of Laminin-211-Deficient CMD Type 1A-Patients
Kölbel, Frontiers in neurology 2019 - “...Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR Q8NDH3 1/1 0.00 Probable aminopeptidase NPEPL1 ( NPEPL1 ) Nucleus 0.13 2.90 Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides P24043 50/48 0.00 Laminin subunit alpha-2 ( LAMA2...”
- Combined proteomic and transcriptomic interrogation of the venom gland of Conus geographus uncovers novel components and functional compartmentalization
Safavi-Hemami, Molecular & cellular proteomics : MCP 2014 - “...P13277 Q03168 Q4VC17 Q26563 P55110 XP_002738143 P37892 Q80W54 Q8NDH3 A6H611 Q20176 A6QPT7 Q80Z60 Q22523 Q9R014 Q26534 P70669 P79171 Q0VGW4 Q5G269 P12955 O75976...”
- Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome
Lange, Journal of proteome research 2014 - “...P14735 M16.002 kellbloodgroupglycoprotein P23276 M13.090 leukotrieneA-4hydrolase P09960 M01.004 Lys-63-specificdeubiquitinaseBRCC36 P46736-2 M67.004 nardilysin O43847 M16.005 probableaminopeptidaseNPEPL1 Q8NDH3 M17.006 probableubiquitincarboxyl-terminalhydrolaseFAF-X Q93008 C19.017 prolylendopeptidase P48147 S09.001 proteasomesubunitbetatype-5 P28074 T01.012 proteasomesubunitbetatype-6 P28072 T01.010 proteasomesubunitbetatype-7 Q99436 T01.011 proteasomesubunitbetatype-9 P28065 T01.013 proteinDJ-1 Q99497 C56.002 secernin-1 Q12765 C69.003 secernin-2 Q96FV2 C69.004 secernin-3 Q0VDG4...”
- The Concise Guide to PHARMACOLOGY 2013/14: enzymes.
Alexander, British journal of pharmacology 2013 - “...Aminopeptidase-like 1 HGNC, UniProt ANPEP, P15144 C9orf3, Q8N6M6 , Q6Q4G3 RNPEP, Q9H4A4 RNPEPL1, Q9HAU8 NPEPL1, Q8NDH3 EC number 3.4.11.2 3.4.11.- 3.4.11.- 3.4.11.6 3.4.11.- 3.4.11.- Nomenclature Endoplasmic reticulum aminopeptidase 1 Endoplasmic reticulum aminopeptidase 2 Glutamyl aminopeptidase Leucine aminopeptidase 3 Methionyl aminopeptidase 1 Methionyl aminopeptidase 2 HGNC, UniProt...”
- Novel direct targets of miR-19a identified in breast cancer cells by a quantitative proteomic approach.
Ouchida, PloS one 2012 - “...19a - IMPDH1 P20839 29.81 3 3 6 55 6.43 19a, 19b - 19a NPEPL1 Q8NDH3 18.26 1 1 2 56 6.41 19a, 19b - - 1 AA: amino acid. 2 MW: molecular weight. Verification of the Direct Targets of miR-19a and miR-20a To confirm that...”
- Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera: identification of proteins binding the delta-endotoxin, Cry1Ac of Bacillus thuringiensis
Angelucci, Insect biochemistry and molecular biology 2008 - “...sequences: Bmb025220 , see text; CG7340, AAF32329 ; HsPEL1, Q8NDH3 ; BmAPNL, Q8T105 ; CG32351 , Q9VSM7 ; HsAPN-L, AAH65564 ; Ae Q95PB9 , AAK55416 ; CG11951,...”
- Human spermatozoa contain multiple targets for protein S-nitrosylation: an alternative mechanism of the modulation of sperm function by nitric oxide?
Lefièvre, Proteomics 2007 - “...1.0 1 Matrix metalloproteinase-24 precursor MMP24_HUMAN Q9Y5R2 73.6 1 1.9 1 Probable aminopeptidase NPEPL1 PEPL1_HUMAN Q8NDH3 56.7 1 2.7 1 Probable serine carboxypeptidase CPVL precursor CPVL_HUMAN Q9H3G5 54.4 2 5.9 1 26S protease regulatory subunit 4 PRS4_HUMAN P62191 49.3 2 5.7 1 Proteasome subunit alpha type...”
Q961W5 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
32% identity, 53% coverage
Q3SWX3 Aminopeptidase-like 1 from Bos taurus
36% identity, 61% coverage
A4HUX3 Putative aminopeptidase from Leishmania infantum
LINJ_11_0640 metallo-peptidase, Clan MF, Family M17 from Leishmania infantum JPCM5
32% identity, 61% coverage
- Potential selection of antimony and methotrexate cross-resistance in Leishmania infantum circulating strains
Bernardo, PLoS neglected tropical diseases 2024 - “...300018100 Pyridoxal kinase 47.5 46.64 0.86 A4HX73 LINF 170005900 Elongation factor 1-alpha 47.64 46.8 0.84 A4HUX3 LINF 110012000 Aminopeptidaseputative 50.57 49.74 0.83 A4IE56 LINF 360050900 Oxidoreductaseputative 45.04 44.22 0.82 A4I154 LINF 250006300 Electron transfer flavoprotein subunit beta 51.28 50.46 0.82 A0A381MM20 LINF 250017900 ATP synthase subunit...”
- “...[ADP-forming] subunit alpha 45.44 45.16 0.28 A4I120 LINF 240027300 3-hydroxy-3-methylglutaryl-CoA synthase- putative 48.54 48.27 0.27 A4HUX3 LINF 110012000 Aminopeptidaseputative 49.4 49.15 0.25 E9AHM9 LINF 330009000 Heat shock protein 8317 45.72 45.5 0.22 A0A381MM90 LINF 280034800 Guanine nucleotide-binding protein subunit beta-like protein 44.55 44.35 0.20 A4HRT1 LINF...”
- Unravelling the proteomic signature of extracellular vesicles released by drug-resistant Leishmania infantum parasites
Douanne, PLoS neglected tropical diseases 2020 - “...(0.659) no 77 LINJ_18_0270 Glycogen synthase kinase 3 putative 2.7 no no (0.499) no 78 LINJ_11_0640 Putative aminopeptidase 2.7 no no (0.499) no 79 LINJ_10_0210 Putative nucleolar protein 2.7 no no (0.396) no 80 LINJ_29_2680 Putative serine/threonine-protein kinase 2.7 no yes (0.613) no 81 LINJ_32_2330 SAC3/GANP/Nin1/mts3/eIF-3...”
P58475 Peptidase B from Yersinia pestis
YPO2889 peptidase B from Yersinia pestis CO92
y1342 putative peptidase from Yersinia pestis KIM
WP_002209829 aminopeptidase PepB from Yersinia pestis KIM10+
36% identity, 67% coverage
YPTB2852 peptidase B from Yersinia pseudotuberculosis IP 32953
36% identity, 67% coverage
- IscR is essential for yersinia pseudotuberculosis type III secretion and virulence
Miller, PLoS pathogens 2014 - “...Biogenesis (11) YPTB0744 Fe-S insertion protein yadR/erpA 6.8 YPTB2851 enhanced serine sensitivity protein sseB 6.6 YPTB2852 peptidase B pepB 10.1 YPTB2853 Fe-S assembly protein iscX/yfhJ 10.8 YPTB2854 Isc system ferredoxin fdx 10.9 YPTB2855 Fe-S assembly chaperone hscA 9.3 YPTB2856 Fe-S assembly chaperone hscB 10.0 YPTB2857 Fe-S...”
Q7K5K9 leucyl aminopeptidase (EC 3.4.11.1) from Drosophila melanogaster (see paper)
34% identity, 54% coverage
R4G844 Putative aminopeptidase of the m17 family from Rhodnius prolixus
35% identity, 62% coverage
5ntdG / Q385B0 Structure of leucyl aminopeptidase from trypanosoma brucei in complex with bestatin (see paper)
33% identity, 62% coverage
- Ligands: manganese (ii) ion; 2-(3-amino-2-hydroxy-4-phenyl-butyrylamino)-4-methyl-pentanoic acid; bicarbonate ion (5ntdG)
5nthA / Q4QH17 Structure of leucyl aminopeptidase from leishmania major in complex with actinonin (see paper)
30% identity, 60% coverage
- Ligands: bicarbonate ion; manganese (ii) ion; actinonin (5nthA)
B7QLQ7 Leucine aminopeptidase, putative from Ixodes scapularis
33% identity, 60% coverage
AMPL_CAEEL / P34629 Leucine aminopeptidase 1; EC 3.4.11.1 from Caenorhabditis elegans (see paper)
NP_498854 Leucine aminopeptidase 1 from Caenorhabditis elegans
32% identity, 64% coverage
- function: Probably acts as a digestive enzyme.
catalytic activity: Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
cofactor: Zn(2+) (Binds 2 Zn(2+) ions per subunit.) - Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity
Guo, Veterinary research 2020 - “...(RJW66865), Toxoplasma gondii (KFG62794.1), Plasmodium vinckeipetteri (EUD71337), Echinococcus granulosus (CDS20652), Brugia malayi (CTP81255), Caenorhabditis elegans (NP_498854). Two sequences of Homo sapiens (AAD17527) and Mus musculus (NP_077754) were used as the outgroups. The phylogenetic analysis was performed in MEGA 7.0 based on the Neighbour-joining (NJ) method as...”
2hc9A / P34629 Structure of caenorhabditis elegans leucine aminopeptidase-zinc complex (lap1)
32% identity, 64% coverage
- Ligands: zinc ion; bicarbonate ion (2hc9A)
SO0876 peptidase B from Shewanella oneidensis MR-1
34% identity, 63% coverage
- Use of microarrays with different probe sizes for monitoring gene expression
He, Applied and environmental microbiology 2005 - “...conditions tested (45C and 50% formamide). Eight ORFs (SO0876, SO0991, SO1193, SO1277, SO1387, SO2320, SO2697, and SO3207) were detected by the 70-mer, 60-mer,...”
A7SGM8 Cytosol aminopeptidase domain-containing protein from Nematostella vectensis
31% identity, 43% coverage
B7P2N5 Leucine aminopeptidase, putative from Ixodes scapularis
43% identity, 31% coverage
K4GLE3 Dipeptidase B-like protein from Callorhinchus milii
31% identity, 43% coverage
- Mass Spectrometry Analysis of Shark Skin Proteins
Bachar-Wikstrom, International journal of molecular sciences 2023 - “...protein A0A401RYU8 Proteasome subunit alpha type Accession Number Protein metabolism H9LEQ0 Haptoglobin A0A401S4Q4 Creatine kinase K4GLE3 Dipeptidase B-like protein A0A401NHT1 Sushi domain-containing protein A0A401NPB6 Cystatin kininogen-type domain-containing protein A0A401Q2J1 Argininosuccinate lyase A0A401PTT0 Cathepsin L (fragment) A0A401SZ08 Aspartate aminotransferase (fragment) A0A401P304 Argininosuccinate synthase Q6EE48 Cathepsin B (fragment)...”
A0A2I7M6F6 leucyl aminopeptidase (EC 3.4.11.1) from Taenia pisiformis (see paper)
30% identity, 43% coverage
C6FWG9 leucyl aminopeptidase (EC 3.4.11.1) from Clonorchis sinensis (see 2 papers)
34% identity, 39% coverage
SO1117, SO_1117 cytosol aminopeptidase, putative from Shewanella oneidensis MR-1
41% identity, 28% coverage
- Survival of Anaerobic Fe2+ Stress Requires the ClpXP Protease
Bennett, Journal of bacteriology 2018 - “...SO_0988 SO_1221 SO_2591 SO_0274 SO_2587 SO_3363 SO_1016 SO_1117 SO_1341 SO_0107 SO_3097 Fe3 Fe-S, Mo5 Purine nucleoside, phosphate Z-ring binding Mg2 Zn2,...”
- Knock-out of SO1377 gene, which encodes the member of a conserved hypothetical bacterial protein family COG2268, results in alteration of iron metabolism, increased spontaneous mutation and hydrogen peroxide sensitivity in Shewanella oneidensis MR-1
Gao, BMC genomics 2006 - “...identified proteins, those exhibiting lower gel abundance in the mutant, included SO1637 (bacterial surface antigen), SO1117 (putative cytosol aminopeptidase), SO4749 (AtpA, alpha subunit of ATP synthase F1), and SO3466 (RibH, beta subunit of riboflavin synthase). Note that consistent with microarray data, the lower gel abundance of...”
- “...surface antigen 21077 (2592) 10347 (2287) 333 SO1377 conserved hypothetical protein 49308 (5917) 0 422 SO1117 putative cytosol aminopeptidase 21725 (1716) 10017 (771) 426 SO2407 conserved hypothetical protein 71070 (11798) 120011 (11641) 433 SO2767 asnB-1 asparagine synthetase B, glutamine-hydrolyzing 49614 (5917) 0 482 SO4749 atpA ATP...”
PSHAa2388 putative cytosol aminopeptidase from Pseudoalteromonas haloplanktis TAC125
29% identity, 58% coverage
Smp_083870, XP_018645401 PwLAP aminopeptidase (M17 family) from Schistosoma mansoni
43% identity, 25% coverage
- Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni
Maggioli, Molecular and biochemical parasitology 2018 (PubMed)- GeneRIF: The enzymes differed in activity against diagnostic substrates, including leucine, methionine and arginine, with an optimal pH of 8.0. The activity increased in the presence of Mg(+2) and Mn(+2), and was inhibited by bestatin, a specific inhibitor of aminopeptidase.
- “...Herein, two LAPs, SmLAP1 (Smp_030000) and SmLAP2 (Smp_083870) of the human blood fluke Schistosoma mansoni were cloned, expressed, purified and biochemically...”
- Proteomic identification of IPSE/alpha-1 as a major hepatotoxin secreted by Schistosoma mansoni eggs
Abdulla, PLoS neglected tropical diseases 2011 - “...dependent epimerase/dehydratase Smp_089370 4 16 2.19 7.10E-06 29.8 5, 9, 10 PwLAP aminopeptidase (M17 family) Smp_083870 7 13 1.49 2.90E-05 59.7 27, 28 annexin Smp_045560 5 18 2.07 2.30E-05 36.9 19, 20 ++ SmVAL26 Smp_154260 2 15 2.52 1.40E-04 20.5 10, 11 proteasome catalytic subunit 2...”
- RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs
Rinaldi, Molecular and biochemical parasitology 2009 - “...to the P91803 query termed Sm LAP1 (GeneDB Smp_030000), and the other termed Sm LAP2 (Smp_083870). The genomic scaffolds containing the hits were visualized in the Genome Browser available at the S. mansoni GeneDB, www.genedb.org/genedb/smansoni/ . A similar search was performed on Schistosoma japonicum using as...”
- “...Gene DB Smp_030000) ( Sm LAP1) and leucine aminopeptidase 2 ( S. mansoni Gene DB Smp_083870) ( Sm LAP2) expression was investigated by RT-PCR using gene specific primers (F: 5-CAT TCC CAG AAC ATC CAT CAA A-3; R: 5-CAT TAT CCA TCA CAG CCG TGA A-3...”
A1Z0K2 leucyl aminopeptidase (EC 3.4.11.1) from Paragonimus westermani (see paper)
36% identity, 38% coverage
B7PA58 Cytosol aminopeptidase domain-containing protein (Fragment) from Ixodes scapularis
29% identity, 55% coverage
- Sialotranscriptomics of the argasid tick Ornithodoros moubata along the trophogonic cycle
Oleaga, PLoS neglected tropical diseases 2021 - “...549.06 3,012.68 1.599.81 Peptidase KRT86698 1 0.67 3.12 2.21 Uncharacterized proteins B7QEM8, B7QM92, B7PKP5, B7Q5E1 B7PA58 27 318.12 4,586.34 1.404.91 Salivary gland metalloprotease DAA34197, DAA34198, B7P187 DAA34210, DAA34252, DAA34264, ISCW014977, ISCW023633, EEC18166, EEC19961, AAZ39659, AAZ39660 38 200.55 1,195.96 1.264.74 Secreted metalloprotease B7QM91, AAM93652 AAM93653, AAT92201 16...”
- Midgut proteome of an argasid tick, Ornithodoros erraticus: a comparison between unfed and engorged females.
Oleaga, Parasites & vectors 2015 - “...0.17 Unfed, Fed F0J8F6 - Metallopeptidase (Fragment) Pa4 - 2 3 0.22 0.41 Unfed, Fed B7PA58 IscW_ISCW003100 Putative uncharacterized protein Pa5 Metalloexopeptidase 2 2 0.14 0.14 Unfed, Fed Q6U8A8 - Serine protease-like protein Pa6 Serine-type endopeptidase 4 4 0.96 0.75 Unfed, Fed Q09JL3 - Signal peptidase...”
Q27245 Putative aminopeptidase W07G4.4 from Caenorhabditis elegans
38% identity, 29% coverage
Q17GL0 AAEL002978-PA from Aedes aegypti
37% identity, 27% coverage
Smp_030000, XP_018651318 leucine aminopeptidase (M17 family) from Schistosoma mansoni
30% identity, 41% coverage
- Schistosoma mansoni Adult Worm Protective and Diagnostic Proteins in n-Butanol Extracts Revealed by Proteomic Analysis
Sulbarán, Pathogens (Basel, Switzerland) 2021 - “...63 , 64 ], PDI (Smp_056760) [ 65 ], as well as the modulatory LAP (Smp_030000) [ 66 , 67 , 68 ]. Trx and PDI have a thioredoxin domain. (7) An isolated link was seen between CALR (Smp_030370) [ 69 ] and Mt TRx (Smp_037530)...”
- “...Smp_017730 200 kDa GPI-anchored surface protein (Sm200) Smp_024110 Enolase / Phosphopyruvate hydratase ( ENO ) Smp_030000 Leucine aminopeptidase ( M17 )/ putative cytosol aminopeptidase ( LAP ) Smp_030370 Calreticulin ( CALR ) Smp_031770 Tropomyosin - 2 ( TPM - 2 ) Smp_034840 14-3-3 protein homolog 2...”
- Single-cell deconstruction of stem-cell-driven schistosome development
Nanes, Trends in parasitology 2021 - “...ciliated neurons 7b2 (Smp_073720); p25 (Smp_097490) kk7 + neurons kk7 (Smp_194830) Parenchymal cb2 (Smp_141610); lap (Smp_030000); serpin (Smp_090080); tgfbi (Smp_212710) Intestinal cb1.1 (Smp_103610); cb1.2 (Smp_067060); ctsl (Smp_343260); hmgbs (Smp_075800) Tegumental syncytial annexin b2 (Smp_077720); calpain (Smp_214190); gtp-4 (Smp_105410); npp-5 (Smp_153390); sm25 (Smp_346900); tal (Smp_045200); tsp2 (Smp_335630)...”
- Single-cell atlas of the first intra-mammalian developmental stage of the human parasite Schistosoma mansoni
Diaz, Nature communications 2020 - “...f vitellaria. White arrows indicate positive cells. Single confocal sections shown. g , h lap (Smp_030000) is expressed in both parenchyma and in the g gut primordia as well as h adult gut, shown by double FISH with the gut cathepsin B (Smp_103610). i Schematic that...”
- “...Fig. 7fj ). In Parenchymal 2 cells, we found that leucine aminopeptidase ( lap ) (Smp_030000) was expressed in the primordial gut expressing cathepsin B (Smp_103610) 66 and surrounding parenchymal tissue (Fig. 4g ). Such mixed gut/parenchymal expression was also observed in adult parasites (Fig. 4h...”
- Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni
Maggioli, Molecular and biochemical parasitology 2018 (PubMed)- GeneRIF: The enzymes differed in activity against diagnostic substrates, including leucine, methionine and arginine, with an optimal pH of 8.0. The activity increased in the presence of Mg(+2) and Mn(+2), and was inhibited by bestatin, a specific inhibitor of aminopeptidase.
- “...fluke Fasciola hepatica. Herein, two LAPs, SmLAP1 (Smp_030000) and SmLAP2 (Smp_083870) of the human blood fluke Schistosoma mansoni were cloned, expressed,...”
- Proteomic identification of IPSE/alpha-1 as a major hepatotoxin secreted by Schistosoma mansoni eggs
Abdulla, PLoS neglected tropical diseases 2011 - “...expressed protein Smp_170410 6 19 2.19 2.90E-06 29.3 25, 26, 29 leucine aminopeptidase (M17 family) Smp_030000 6 11 2.28 1.30E-05 56.7 2628 methylthioadenosine phosphorylase Smp_028190 3 11 3.55 1.50E-07 34.6 12, 13 superoxide dismutase [Mn] Smp_056440 3 11 2.68 1.20E-05 24.3 15 ++ L-lactate dehydrogenase, putative...”
- RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs
Rinaldi, Molecular and biochemical parasitology 2009 - “...hits with the queries, one being identical to the P91803 query termed Sm LAP1 (GeneDB Smp_030000), and the other termed Sm LAP2 (Smp_083870). The genomic scaffolds containing the hits were visualized in the Genome Browser available at the S. mansoni GeneDB, www.genedb.org/genedb/smansoni/ . A similar search...”
- “...iScript cDNA Synthesis Kit (BioRad, Hercules, CA). Leucine aminopeptidase 1 ( S. mansoni Gene DB Smp_030000) ( Sm LAP1) and leucine aminopeptidase 2 ( S. mansoni Gene DB Smp_083870) ( Sm LAP2) expression was investigated by RT-PCR using gene specific primers (F: 5-CAT TCC CAG AAC...”
P91803 Putative cytosol aminopeptidase (Fragment) from Schistosoma mansoni
30% identity, 40% coverage
- RNA interference targeting leucine aminopeptidase blocks hatching of Schistosoma mansoni eggs
Rinaldi, Molecular and biochemical parasitology 2009 - “...24 ]. 2.2 Bioinformatics characterization of schistosome leucine aminopeptidase The protein sequences of S. mansoni (P91803) [ 19 ] and Fasciola hepatica (AY064459) [ 25 ] LAPs described previously were used as queries for blast searches using the Omniblast interface available at the S. mansoni Genome...”
- “...Only two genomic regions produced significant hits with the queries, one being identical to the P91803 query termed Sm LAP1 (GeneDB Smp_030000), and the other termed Sm LAP2 (Smp_083870). The genomic scaffolds containing the hits were visualized in the Genome Browser available at the S. mansoni...”
C6KGZ9 Leucine amino peptidase from Fasciola gigantica
29% identity, 42% coverage
Q17TZ3 Leucyl aminopeptidase from Fasciola hepatica
29% identity, 42% coverage
C1L5C5 Putative aminopeptidase W07G4.4 from Schistosoma japonicum
30% identity, 40% coverage
- Effect of Praziquantel on Schistosoma mekongi Proteome and Phosphoproteome
Chienwichai, Pathogens (Basel, Switzerland) 2020 - “...39.5 6.56 447 53.4 3.27 7 A0A095AJN4 Enolase 46.6 6.34 130 27.9 2.56 Protease 8 C1L5C5 Putative aminopeptidase W07G4.4 56.2 7.14 360 25.7 2.31 9 A0A094ZYF3 Putative aminopeptidase W07G4.4 57.6 7.55 169 25.3 2.16 10 P43157 Cathepsin B 38.7 7.14 522 30.4 2.33 11 A0A095B296 Cathepsin...”
- “...Down Proteins involving with protein folding and proteolysis 48 P43157 Cathepsin B Up Up 49 C1L5C5 Putative aminopeptidase W07G4.4 Up 50 A0A094ZYF3 Putative aminopeptidase W07G4.4 Up 51 A0A095B296 Cathepsin B-like cysteine proteinase Up 52 C1LA34 Cathepsin B-like cysteine proteinase precursor Up 53 C1L8N7 Putative Lysosomal Pro-X...”
C6FWH0 leucyl aminopeptidase (EC 3.4.11.1) from Clonorchis sinensis (see paper)
28% identity, 40% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory