PaperBLAST
PaperBLAST Hits for sp|Q9I685|SAHH_PSEAE Adenosylhomocysteinase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=ahcY PE=1 SV=1 (469 a.a., MSAVMTPAGF...)
Show query sequence
>sp|Q9I685|SAHH_PSEAE Adenosylhomocysteinase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=ahcY PE=1 SV=1
MSAVMTPAGFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILGCI
HMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAWKGETEEEYEWCI
EQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLERIHGITEETTTGVHRLLDMLKNGT
LKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRGTDHLLSGKQALVIGYGDVGKGSSQSL
RQEGMIVKVAEVDPICAMQACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGN
VNVCDANMLKALKKRAVVCNIGHFDNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFDAH
NDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYADLPAAEKAKRLSV
EVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVSVEGPFKPDTYRY
Running BLASTp...
Found 199 similar proteins in the literature:
Q9I685 adenosylhomocysteinase (EC 3.3.1.1) from Pseudomonas aeruginosa (see paper)
PA0432 S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
- Distinct transcriptome and traits of freshly dispersed Pseudomonas aeruginosa cells
Kalia, mSphere 2024 - “...subunit F Upregulated 1.296643 Upregulated 0.635864 PA0283 Sulfate binding protein precursor Upregulated 0.569928 Upregulated 2.084621 PA0432 SahH-S-adenosyl-L-homocysteine hydrolase Upregulated 0.555658 Upregulated 0.100262 PA0508 Probable acyl-CoA dehydrogenase Upregulated 1.638803 Upregulated 1.018265 PA0546 Methionine adenosyltransferase Upregulated 0.776164 Upregulated 0.340434 PA0548 Transketolase Upregulated 0.64417 Upregulated 0.129716 PA0729.1 tRNA-Gly Upregulated...”
- In vitro evolution of Pseudomonas aeruginosa AA2 biofilms in the presence of cystic fibrosis lung microbiome members
Vandeplassche, Scientific reports 2019 - “...Y349X D133A V72M 41 4 2 mexL PA3678 SNP GA (12) 221690 A26T 23 sahH PA0432 SNP GA (1) 95660 297 nt upstream *c 99 pilA PA4525 Del CTACCA (6) 118729 T154_T155del 97 T18 L1 M lasR PA1430 Del G (2) 294159 K182RfsX27 100 pqsR PA1003...”
- “...CGAGCTGACCGC (11) 20762 (11) 20879 (11) 21061 I136AfsX208 L98FfsX34 A34_S37del *d 69 11 7 sahH PA0432 SNP GA (1) 95660 297 nt upstream 56 pilA PA4525 Del CTACCA (6) 118729 T154_T155del 92 T18 L2 C lasR PA1430 In +A (2) 294001 S129KfsX104 94 pqsR PA1003 SNP...”
- Proteomic Response of Pseudomonas aeruginosa PAO1 Adhering to Solid Surfaces
Guilbaud, Frontiers in microbiology 2017 - “...Aer2 PA0176 aer2 6.6 5.8 15.0 1.2E-04 1.7E-05 Amino acid biosynthesis and metabolism S-adenosyl-L-homocysteine hydrolase PA0432 sahH 9.6 12.0 18.0 7.4E-04 proline dehydrogenase PutA PA0782 putA 17.0 18.6 10.4 8.6E-03 1.6E-03 Central intermediary metabolism malate synthase G PA0482 glcB 13.0 12.4 20.4 7.7E-03 3.5E-03 Carbon compound...”
- Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa
Dötsch, Antimicrobial agents and chemotherapy 2009 - “...PA0247 PA0287 PA0316 PA0339 PA0381 PA0387 PA0407 PA0415 PA0432 PA0460 PA0479 PA0533 PA0545 PA0596 PA0597 PA0610 PA0666 PA0667 PA0807 PA0842 PA0908 PA0944 PA0958...”
- Novel genetic determinants of low-level aminoglycoside resistance in Pseudomonas aeruginosa
Schurek, Antimicrobial agents and chemotherapy 2008 - “...growth defect PAO1 ortholog(s) of mutant gene PA0432 PA1547 PA1548 PA1588 PA4429 PA4430 PA5070 Gene name Gene description sahH S-Adenosyl-L-homocysteine...”
- Insertions of the transposon Tn1 into the Pseudomonas aeruginosa chromosome
Krishnapillai, Genetics 1981 - “...each cross). It was found that the bZa+ of PA0432 and PA0435 were co-transducible with catAl+ (57% and 51%, respectively), while all the other bla+ insertions...”
- “...identical P. aeruginosa CHROMOSOME INSERTIONS 501 PA0429 PA0431 PA0432 PA0435 FIGURE 1.-Linkage data for the location of T n l inserts in PA0429, POA431-PA0435....”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...the following accession numbers Thermotoga maritima (O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...code): Lupinus luteus (Q9SP37), Plasmodium falciparum (P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD + binding in the...”
PA14_05620 S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa UCBPP-PA14
100% identity, 100% coverage
8cfuA / Q9I685 Crystal structure of s-adenosyl-l-homocysteine hydrolase from p. Aeruginosa in complex with f2x-entry library fragment h02
100% identity, 100% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenine; 3-cyclopentyl-1-(piperazin-1-yl)propan-1-one (8cfuA)
AFE_0534 adenosylhomocysteinase from Acidithiobacillus ferrooxidans ATCC 23270
77% identity, 100% coverage
D0CC64 Adenosylhomocysteinase from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
HMPREF0010_02344 adenosylhomocysteinase from Acinetobacter baumannii ATCC 19606 = CIP 70.34 = JCM 6841
76% identity, 99% coverage
ACIAD2282 S-adenosyl-L-homocysteine hydrolase from Acinetobacter sp. ADP1
74% identity, 99% coverage
A1S_2334 S-adenosyl-L-homocysteine hydrolase from Acinetobacter baumannii ATCC 17978
76% identity, 93% coverage
- Novel Genes Required for Surface-Associated Motility in Acinetobacter baumannii
Blaschke, Current microbiology 2021 - “...N.s. N.s. DNA modification/repair/uptake A1S_0222 aamA Adenine-specific methyltransferase ++ Y *** **** N.s. S*** S** A1S_2334 sahH S -Adenosyl- l -homocysteine hydrolase +++ Y * N.s. R*** N.s. N.s. A1S_2587 ruvA Holliday junction helicase subunit A ++ Y N.s. **** R*** N.s. S** A1S_2610 comEC Competence...”
- “...A1S_1055 26.6 21.3 0.29 0.14 1.3 1.6 A1S_2182 gidA 37.3 24.0 0.19 0.25 3.0 4.0 A1S_2334 sahH 74.6 0.18 1.8 A1S_2587 ruvA 53.3 0.19 1.0 A1S_2610 comEC 29.3 5.3 0.23 0.10 1.6 0.6 A1S_2761 prpF 42.6 14.6 0.25 0.16 0.8 0.5 A1S_3026 106.6 64.0 0.42 0.46...”
A8U0F7 Adenosylhomocysteinase from alpha proteobacterium BAL199
61% identity, 99% coverage
lpg2021 adenosylhomocysteinase from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
57% identity, 98% coverage
PB7211_1045 adenosylhomocysteinase from Candidatus Pelagibacter sp. HTCC7211
58% identity, 98% coverage
- mdRNA-Seq analysis of marine microbial communities from the northern Red Sea
Hou, Scientific reports 2016 - “...tolerance regulatory protein actr acpP PB7211_217 613491 613746 + 292 124 acyl carrier protein ahcY PB7211_1045 368453 369739 + 291 632 adenosylhomocysteinase PB7211_767 PB7211_767 1161417 1163849 284 257 dimethylglycine dehydrogenase rpoZ PB7211_373 739908 740423 + 265 222 dna-directed rna polymerase omega subunit gidA PB7211_100 225271 227142...”
P27604 Adenosylhomocysteinase from Caenorhabditis elegans
58% identity, 97% coverage
XP_647635 AdoHyc hydrolase from Dictyostelium discoideum AX4
59% identity, 97% coverage
CBU_2031 adenosylhomocysteinase from Coxiella burnetii RSA 493
56% identity, 98% coverage
6aphA / A0A077EDS4 Crystal structure of adenosylhomocysteinase from elizabethkingia anophelis nuhp1 in complex with NAD and adenosine
56% identity, 98% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (6aphA)
XP_809153 S-adenosylhomocysteine hydrolase, putative from Trypanosoma cruzi
Q4D455 Adenosylhomocysteinase from Trypanosoma cruzi (strain CL Brener)
57% identity, 98% coverage
- Subcellular proteomics of Trypanosoma cruzi reservosomes
Sant'Anna, Proteomics 2009 - “...(1) 5 Cysteine peptidase XP_821954 Lysosomal integral to membrane, proteolysis Y (1) 6 S-Adenosylhomocysteine hydrolase XP_809153 Adenosylhomocysteine hydrolase activity N 7 Cysteine peptidase C XP_812694 Lysosomal, proteolysis N 8 Calpain cysteine peptidase XP_816696 Calcium-dependent cysteine-type endopeptidase activity N 9 Membrane-bound acid phosphatase XP_808329 Catalytic activity N...”
- Secretome analysis of Trypanosoma cruzi by proteomics studies
Brossas, PloS one 2017 - “...putative Q4E0N6 14.7 40S ribosomal protein S18, putative Q4E093 17.5 Actin, putative Q4D7A6 38.1 Adenosylhomocysteinase Q4D455 48.4 ADP-ribosylation factor 1, putative Q4D7Y8 20.7 Alpha tubulin, putative Q4CLA1 49.8 Arginine kinase, putative Q4CWA5 40.2 ATP synthase subunit beta Q4DTX7 55.7 Beta tubulin, putative Q4DQP2 49.7 Calcium-binding protein,...”
Q7YUF0 adenosylhomocysteinase (EC 3.3.1.1) from Trypanosoma cruzi (see paper)
56% identity, 98% coverage
A0A3Q9QZN1 adenosylhomocysteinase (EC 3.3.1.1) from Penaeus vannamei (see paper)
56% identity, 97% coverage
Q803T5 adenosylhomocysteinase (EC 3.3.1.1) from Danio rerio (see paper)
NP_954688 adenosylhomocysteinase from Danio rerio
57% identity, 97% coverage
LEPBI_II0185 S-adenosylhomocysteine hydrolase from Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)'
58% identity, 100% coverage
- The sigma factor σ54 is required for the long-term survival of Leptospira biflexa in water
Zhang, Molecular microbiology 2018 - “...COG1629P Putative TonB-dependent receptor protein LA_2242 LBL_1447 LEPBI_I2761 3.17 - Putative signal peptide LA_2241 LBL_1445 LEPBI_II0185 3.00 COG0499H S-adenosyl-L-homocysteine hydrolase LB_106 LBL_4105 LEPBI_I2678 2.99 - Hypothetical protein No hit No hit LEPBI_I2246 2.97 - Hypothetical protein No hit No hit LEPBI_I2762 2.66 COG3182S Putative iron-regulated membrane...”
XP_001639319 adenosylhomocysteinase from Nematostella vectensis
55% identity, 99% coverage
LB_106, LIC_20083 adenosylhomocysteinase from Leptospira interrogans serovar Lai str. 56601
LB106 S-adenosyl-L-homocysteine hydrolase from Leptospira interrogans serovar lai str. 56601
LIC20083 S-adenosylhomocysteine hydrolase from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
57% identity, 100% coverage
- The sigma factor σ54 is required for the long-term survival of Leptospira biflexa in water
Zhang, Molecular microbiology 2018 - “...LA_2242 LBL_1447 LEPBI_I2761 3.17 - Putative signal peptide LA_2241 LBL_1445 LEPBI_II0185 3.00 COG0499H S-adenosyl-L-homocysteine hydrolase LB_106 LBL_4105 LEPBI_I2678 2.99 - Hypothetical protein No hit No hit LEPBI_I2246 2.97 - Hypothetical protein No hit No hit LEPBI_I2762 2.66 COG3182S Putative iron-regulated membrane protein; putative membrane protein LA_2440...”
- Pathogenic Leptospires Modulate Protein Expression and Post-translational Modifications in Response to Mammalian Host Signals
Nally, Frontiers in cellular and infection microbiology 2017 - “...Acyl-CoA dehydrogenase (LIC_10970) 580, 581 6.5E-06, 2.9E-06 4.69, 3.83 gi|45602637 ( AAS72112.1 ) S-adenosylhomocysteine hydrolase (LIC_20083) 1,606 0.00012 4.61 gi|45601539 ( AAS71020.1 ) Response regulator (LIC_12454) 1,144 0.000059 4.5 gi|45600133 ( AAS69619.1 ) Conserved hypothetical protein (LIC_11009) None predicted 260, 256 0.0001, 0.0031 4.14, 3.2 gi|45601779...”
- Transcriptional responses of Leptospira interrogans to host innate immunity: significant changes in metabolism, oxygen tolerance, and outer membrane
Xue, PLoS neglected tropical diseases 2010 - “...0.95 5 hypothetical protein 4 LB099 1.52 14.29 6.67 3.54 1.34 2.27 hypothetical protein 5 LB106 2.55 5.26 4.17 2.17 2.27 3.57 S-adenosyl-L-homocysteine hydrolase (catalyzes the formation of L-homocysteine from S-adenosyl-L-homocysteine) 2 LB327 0.73 4.76 3.85 4 7.14 4.55 aconitate hydratase The ORFs down-regulated at least...”
- Synthesis of autoinducer 2 by the lyme disease spirochete, Borrelia burgdorferi
Babb, Journal of bacteriology 2005 - “...of SAH detoxification via an SAH hydrolase homolog, ORF LB106. Of these four spirochetes, only B. burgdorferi encodes a recognizable homolog of LuxS, which we...”
- Pathogenic Leptospires Modulate Protein Expression and Post-translational Modifications in Response to Mammalian Host Signals
Nally, Frontiers in cellular and infection microbiology 2017 - “...proteins involved in RNA transcription and degradation (LIC11004, LIC13073, LIC12701, & LIC12636), amino acid metabolism (LIC20083, LIC13244, $& LIC10736), two component systems signal transduction systems (LIC12454 & LIC11194) and energy storage (LIC11241 & LIC11243). Table 3 Proteins less abundant ( p < 0.05, fold > 1.25)...”
Bm1_48165 Uncharacterized protein from Brugia malayi
56% identity, 97% coverage
- Yeast-Based High-Throughput Screens to Identify Novel Compounds Active against Brugia malayi
Bilsland, PLoS neglected tropical diseases 2016 - “...Bm NMT), Bm1_01925 ( Bm PGK), Bm1_29130 ( Bm TPI), Bm1_ 49000 ( Bm PIS), Bm1_48165 ( Bm SAH), Bm1_38705 ( Bm SEC53), Bm1_11585 ( Bm ADE13) and Bm1_23075 ( Bm CDC8) were PCR-amplified from an adult Brugia malayi cDNA library, kindly donated by the Filariasis...”
- “...energy metabolism PGK1 triose-phosphate isomerase (TPI) Bm1_29130 WUBG_07449 P60174 Glycolysis, energy metabolism TPI1 adenosyl homocysteinase Bm1_48165 WUBG_05842 P23526 Cellular lipid homoeostasis SAH1 Inorganic pyrophosphatase Bm1_16955 WUBG_14547 Q15181 Rapid exchange of oxygen from Pi with water IPP1 Phospho mannomutase Bm1_38705 WUBG_05311 O15305 Folding and glycosylation of secretory...”
- Identification of attractive drug targets in neglected-disease pathogens using an in silico approach
Crowther, PLoS neglected tropical diseases 2010 - “...Bm1_52850 mannose-6-phosphate isomerase, class I family protein 168 23 Bm1_12165 methionyl-tRNA synthetase, putative 168 23 Bm1_48165 Adenosylhomocysteinase, putative 168 23 Bm1_32455 mannose-6-phosphate isomerase, class I family protein 168 23 Bm1_22825 Protein prenyltransferase alpha subunit repeat containing protein 168 23 Bm1_14125 proteasome subunit beta type 1, putative...”
EGR_05478 Adenosylhomocysteinase A from Echinococcus granulosus
56% identity, 36% coverage
- Unraveling oxidative stress response in the cestode parasite Echinococcus granulosus
Cancela, Scientific reports 2019 - “...protein S13 EgrG_000856900 0.036 0.019052 0.058467 3.06 6 phosphogluconolactonase EgrG_000445200 0.025 0.0117905 0.029843 2.53 Adenosylhomocysteinase EGR_05478 0.026 0.0077762 0.0193915 2.49 Cathepsin d lysosomal aspartyl protease EgrG_000970500 0.016 0.0158975 0.075669 4.76 Collagen alpha-1(IV) chain EGR_08512 0.0035 0.0142325 0.021473 1.50 Collagen alpha-1(XXIV) chain EGR_03871 0.0018 0.0014417 0.0090935 6.30...”
Q04NN6 Adenosylhomocysteinase from Leptospira borgpetersenii serovar Hardjo-bovis (strain JB197)
LBL_4105 S-adenosylhomocysteine hydrolase from Leptospira borgpetersenii serovar Hardjo-bovis L550
56% identity, 100% coverage
An08g01960 uncharacterized protein from Aspergillus niger
56% identity, 97% coverage
- The International Space Station Environment Triggers Molecular Responses in Aspergillus niger
Blachowicz, Frontiers in microbiology 2022 - “...lysine biosynthesis 1.48 8.47E-03 An02g07250 AgaA Arginase 1.54 2.86E-03 An02g12430 IcdA Isocitrate dehydrogenase 1.59 1.38E-03 An08g01960 Adenosylhomocysteinase 1.62 4.43E-03 An17g00910 Gamma-aminobutyrate transaminase 1.72 5.69E-03 An11g02160 Maleylacetoacetate isomerase 1.83 4.49E-03 An03g04280 Protein with similarity to pyridoxine synthesis component pyroA of Aspergillus nidulans 1.88 9.72E-03 * Log2 fold...”
- Cytosolic streaming in vegetative mycelium and aerial structures of Aspergillus niger
Bleichrodt, Studies in mycology 2013 - “...- Arthrobacter oxidans An07g00010 1176 Similarity to hypothetical protein encoded by An07g00070 - Aspergillus niger An08g01960 1168 Strong similarity to adenosylhomocysteinase - Homo sapiens An14g04920 1167 Triose-phosphate-isomerase tpiA from patent WO8704464-A - Aspergillus niger An11g01630 1167 Strong similarity to thiazole biosynthesis protein nmt2p - Schizosaccharomyces pombe...”
LOC408368 adenosylhomocysteinase from Apis mellifera
57% identity, 97% coverage
Alvin_0320 adenosylhomocysteinase from Allochromatium vinosum DSM 180
55% identity, 98% coverage
- Metabolomic profiling of the purple sulfur bacterium Allochromatium vinosum during growth on different reduced sulfur compounds and malate
Weissgerber, Metabolomics : Official journal of the Metabolomic Society 2014 - “...-homoserine sulfhydrylase (Alvin_1027), MetE cobalamin-independent methionine synthase (Alvin_2262), MetH cobalamin-dependent methionine synthase (Alvin_1622), AhcY adenosylhomocysteinase (Alvin_0320), BchM magnesium protoporphyrin O -methyltransferase (Alvin_2638), MetK S -adenosylmethionine synthetase (Alvin_0318); 0319, methyltransferase type 11 (Alvin_0319). The transcriptomic ( boxes ) (Weissgerber et al. 2013 ), proteomic ( circles )...”
- “...(Sganga et al. 1992 ) and is immediately hydrolytically recycled to homocysteine (catalyzed by AhcY, Alvin_0320). Furthermore, high concentrations of AdoMet are known to inhibit threonine biosynthesis in A. vinosum by negatively influencing homoserine dehydrogenase activity (Sugimoto et al. 1976 ). Taken together, the high demand...”
Q383X0 Adenosylhomocysteinase from Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
55% identity, 98% coverage
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...(P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by Clustal Omega ( https://www.ebi.ac.uk/Tools/msa/clustalo/ ) and Phylodendron ( http://iubioarchive.bio.net/treeapp/treeprint-form.html ). The % indicates the similarity with human protein...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...(P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD + binding in the Rossmann fold-based CBD of SAHases. Residues...”
C0NJC3 Adenosylhomocysteinase from Ajellomyces capsulatus (strain G186AR / H82 / ATCC MYA-2454 / RMSCC 2432)
55% identity, 97% coverage
SAHH_DROME / Q27580 Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Drosophila melanogaster (Fruit fly) (see paper)
57% identity, 97% coverage
- function: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Interacts with AhcyL1; the interaction may negatively regulate Ahcy catalytic activity.
disruption phenotype: RNAi-mediated knockdown results in increased S- adenosyl-homocysteine levels and reduced lifespan.
Q5M9P0 Adenosylhomocysteinase from Mus musculus
58% identity, 97% coverage
Pc16g05080 uncharacterized protein from Penicillium rubens
56% identity, 97% coverage
- A Penicillium rubens platform strain for secondary metabolite production
Pohl, Scientific reports 2020 - “...oxidase encoded by Pc22g20960 was decreased (log2 FC of 1.3). The adenosyl homocysteinase encoded by Pc16g05080 was lower expressed (log2 FC 0.3), putatively providing less adenosine. Also, expression of genes involved in pyrimidine biosynthesis was changed, namely the lower expression of the 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5...”
SAHH_MOUSE / P50247 Adenosylhomocysteinase; AdoHcyase; CUBP; Liver copper-binding protein; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Mus musculus (Mouse) (see paper)
P50247 adenosylhomocysteinase (EC 3.3.1.1) from Mus musculus (see paper)
NP_057870 adenosylhomocysteinase from Mus musculus
Q3TF14 Adenosylhomocysteinase from Mus musculus
Q6P743 Adenosylhomocysteinase from Rattus norvegicus
58% identity, 97% coverage
- function: Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed:7657650). Binds copper ions (PubMed:7657650).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer. Interaction with AHCYL1 (By similarity). - Adenosylhomocysteinase plays multiple roles in maintaining the identity and pluripotency of mouse embryonic stem cells†.
Jiang, Biology of reproduction 2024 (PubMed)- GeneRIF: Adenosylhomocysteinase plays multiple roles in maintaining the identity and pluripotency of mouse embryonic stem cellsdagger.
- Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells.
Boczki, Adipocyte 2024 - GeneRIF: Inhibition of AHCY impedes proliferation and differentiation of mouse and human adipocyte progenitor cells.
- Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target.
Vande, Nature metabolism 2023 - GeneRIF: Metabolic profiling stratifies colorectal cancer and reveals adenosylhomocysteinase as a therapeutic target.
- DJ-1 suppresses ferroptosis through preserving the activity of S-adenosyl homocysteine hydrolase.
Cao, Nature communications 2020 - GeneRIF: Inhibition of DJ-1 potently enhances the sensitivity of tumor cells to ferroptosis inducers both in vitro and in vivo. Metabolic analysis and metabolite rescue assay reveal that DJ-1 depletion inhibits the transsulfuration pathway by disrupting the formation of the S-adenosyl homocysteine hydrolase tetramer and impairing its activity. Study findings show that DJ-1 determines the response of cancer cells to ferroptosis.
- S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling.
Greco, Science advances 2020 - GeneRIF: S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling.
- Epigenetically mediated inhibition of S-adenosylhomocysteine hydrolase and the associated dysregulation of 1-carbon metabolism in nonalcoholic steatohepatitis and hepatocellular carcinoma.
Pogribny, FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 - GeneRIF: These results indicate that epigenetically mediated inhibition of Ahcy expression may be a driving force in causing SAH elevation and subsequent downstream disturbances in transsulfuration and transmethylation pathways during the development and progression of nonalcoholic steatohepatitis.
- H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
Zhou, Nature communications 2015 - GeneRIF: H19 knockdown activates SAHH, leading to increased DNMT3B-mediated methylation of an lncRNA-encoding gene Nctc1 within the Igf2-H19-Nctc1 locus.
- Coupling global methylation and gene expression profiles reveal key pathophysiological events in liver injury induced by a methyl-deficient diet.
Tryndyak, Molecular nutrition & food research 2011 (PubMed)- GeneRIF: Ahcy was identified from coupling of methylation with gene expression data, shed light on the underlying mechanisms of cytosine demethylation under methyl-deficient conditions.
- More
- Decreased methylglyoxal-mediated protein glycation in the healthy aging mouse model of ectopic expression of UCP1 in skeletal muscle
Masania, Redox biology 2023 - “...LIM domain protein 5 0.71 0.037 14. Q564E2 l -lactate dehydrogenase-A 0.72 0.013 Glycolysis 15. P50247 Adenosylhomocysteinase 0.73 0.031 16. Q9DCZ1 GMP reductase 1 0.77 0.039 17. P10649 Glutathione S -transferase M1 0.78 0.032 Metabolizes lipid peroxidation products 18. P47754 F-actin-capping protein subunit alpha-2 0.78 0.013...”
- Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide Isomerase.
Zhang, Cellular and molecular gastroenterology and hepatology 2023 - “...protein subunit alpha-2 3 1 1 P47963 Rpl13 60S ribosomal protein L13 10 2 2 P50247 Ahcy Adenosylhomocysteinase 3 1 1 P50446 Krt6a Keratin type II cytoskeletal 6A 41 41 2 P50516 Atp6v1a V-type proton ATPase catalytic subunit A 2 1 1 P50580 Pa2g4 Proliferation-associated protein...”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...(Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by Clustal Omega ( https://www.ebi.ac.uk/Tools/msa/clustalo/ ) and Phylodendron ( http://iubioarchive.bio.net/treeapp/treeprint-form.html ). The % indicates the similarity with human protein (P23526) calculated using...”
- Proteotyping of knockout mouse strains reveals sex- and strain-specific signatures in blood plasma
Mohammed, NPJ systems biology and applications 2021 - “...negative regulation of complement activation, lectin pathway; stem cell differentiation Ahcy tm1b(EUCOMM)Hmgu Ahcy (269378) HET P50247 P23526 Adenosylhomocysteinase Competitive inhibitor of S -adenosyl- l -methionine-dependent methyl transferase reactions Chronic inflammatory response to antigenic stimulus; circadian sleep/wake cycle; one-carbon metabolic process; response to nutrient; S -adenosylhomocysteine catabolic...”
- Diminazene Aceturate Stabilizes Atherosclerotic Plaque and Attenuates Hepatic Steatosis in apoE-Knockout Mice by Influencing Macrophages Polarization and Taurine Biosynthesis.
Stachowicz, International journal of molecular sciences 2021 - “...mitochondrial 1.06 P24270 Cat Catalase 1.06 Q9WVL0 Gstz1 Maleylacetoacetate isomerase 1.06 Q61176 Arg1 Arginase-1 1.07 P50247 Ahcy Adenosylhomocysteinase 1.08 P30115 Gsta3 Glutathione S-transferase A3 1.09 Q8BVI4 Qdpr Dihydropteridine reductase 1.09 P61922 Abat 4-aminobutyrate aminotransferase, mitochondrial 1.10 P35492 Hal Histidine ammonia-lyase 1.10 Q9QXF8 Gnmt Glycine N-methyltransferase 1.10...”
- Chromatin-Bound Proteome Profiling by Genome Capture.
Aranda, STAR protocols 2020 - “...contain the information for all proteins identified in the experiment. Prey Accession Length (aa) Name P50247 432 AHCY P23198 183 CBX3 P20263 352 POU5F1 The interaction.txt file should contain four tab-separated columns as shown below (but without the header): i) IP name, ii) bait name or...”
- “...should be removed from the file. IP Name Bait Prey Accession Spectral Counts CTL1 CHROM P50247 1 CTL1 CHROM P23198 3 CTL1 CHROM P20263 1 CTL2 CHROM P50247 1 CTL2 CHROM P23198 2 CTL2 CHROM P20263 2 CTL3 CHROM P50247 2 CTL3 CHROM P23198 1 CTL3...”
- Quantitative Proteomic Analysis Reveals That Arctigenin Alleviates Concanavalin A-Induced Hepatitis Through Suppressing Immune System and Regulating Autophagy.
Feng, Frontiers in immunology 2018 - “...16.37 Q8CEE6 Pask PAS domain-containing serine/threonine-protein kinase 10.94 Q9JKV1 Adrm1 Proteasomal ubiquitin receptor ADRM1 7.91 P50247 Ahcy Adenosylhomocysteinase 6.93 Q9QZU9 Ube2l6 Ubiquitin/ISG15-conjugating enzyme E2 L6 5.99 P62874 Gnb1 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 5.33 P50283 Cd7 T-cell antigen CD7 4.75 Q64339 Isg15 Ubiquitin-like protein ISG15...”
- “...0.09 Q8CEE6 Pask PAS domain-containing serine/threonine-protein kinase 0.13 Q9JKV1 Adrm1 Proteasomal ubiquitin receptor ADRM1 0.18 P50247 Ahcy Adenosylhomocysteinase 0.21 Q9QZU9 Ube2l6 Ubiquitin/ISG15-conjugating enzyme E2 L6 0.23 P50283 Cd7 T-cell antigen CD7 0.29 Q64339 Isg15 Ubiquitin-like protein ISG15 0.32 Q9QUJ7 Acsl4 Long-chain-fatty-acidCoA ligase 4 0.33 Q8R3B1 Plcd1...”
- Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses.
Maroto, Journal of extracellular vesicles 2017 - “...0 119.26 0.19 0.35 Annexin A5 Anxa5 P48036 24 0 323.31 0.46 0.33 Adenosylhomocysteinase Ahcy P50247 2 0 4.9712 0.32 0.31 EH domain-containing protein 4 Ehd4 Q9EQP2 8 0 22.779 0.40 0.30 Ras-related protein Rab-1A Rab1A P62821 2 0 14.628 0.17 0.23 14-3-3 protein theta Ywhaq...”
- More
- Integrated Metabolomics and Proteomics Analysis of Urine in a Mouse Model of Posttraumatic Stress Disorder.
Zhou, Frontiers in neuroscience 2022 - “...Q9D3H2 Obp1a Odorant-binding protein 1a 1.67221913 0.01499012 14 A2BHR2 Lcn11 Lipocalin 11 1.54440618 0.01672011 15 Q3TF14 Ahcy Adenosylhomocysteinase 1.82072680 0.01895520 16 A0A0U1RPF4 Hamp2 Hepcidin-2 (Fragment) 2.95442297 0.02102362 17 P10639 Txn Thioredoxin 1.993343846 0.027021861 18 A2CEK6 Mup13 Major urinary protein 11 1.85239538 0.02745480 19 P15947 Klk1 Kallikrein-1...”
- Proteomic Analysis of Mucopolysaccharidosis IIIB Mouse Brain
De, Biomolecules 2020 - “...GA, N Q9R0Q6 Arpc1a Actin-related protein 2/3 complex subunit 1A 2.4 0.00036 CK, N, PM Q3TF14 Ahcy Adenosylhomocysteinase 2.4 0.00329 CK, N Q9CX86 Hnrnpa0 Heterogeneous nuclear ribonucleoprotein A0 2.4 0.00165 N Q9Z1B3 Plcb1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 2.4 0.00671 CK, N, PM O54991 Cntnap1 Contactin-associated protein...”
- Quantitative Proteomics Analysis for the Identification of Differential Protein Expression in Calf Muscles between Young and Old SD Rats Using Mass Spectrometry
Kim, ACS omega 2021 - “...4.4 annexinA2 Q07936 4.4 ubiquinone biosynthesis protein COQ9, mitochondrial Q68FT1 4.4 apolipoproteinH Q5I0M1 4.8 adenosylhomocysteinase Q6P743 5.1 NADH:ubiquinone oxidoreductase subunitB10 D4A0T0 5.7 complementC3 M0RBF1 5.7 dysferlin A0A0G2K7B6 6.1 annexinA5 P14668 6.1 vitaminD-binding protein P04276 6.8 decorin Q01129 6.8 acyl-coenzymeA dehydrogenase, very long chain Q5M9H2 7 3-ketoacyl-CoA...”
Q4WT91 Adenosylhomocysteinase from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
AFUA_1G10130 adenosylhomocysteinase from Aspergillus fumigatus Af293
56% identity, 97% coverage
- The proteomic signature of Aspergillus fumigatus during early development
Cagas, Molecular & cellular proteomics : MCP 2011 - “...29 kDa 48 kDa 45 kDa Q4WLM5 Q4WT91 Q4WJR7 Q4WJ94 Molecular Weight UniProt ID AFUA_2G09790 AFUA_1G09440 AFUA_6G05210 AFUA_2G03010 AFUA_2G10030 AFUA_2G06150...”
- Targeting Methionine Synthase in a Fungal Pathogen Causes a Metabolic Imbalance That Impacts Cell Energetics, Growth, and Virulence
Scott, mBio 2020 - “...episomally overexpress genes that could detoxify those products: the S -adenosyl-homocysteinase lyase encoding gene sahL (AFUA_1G10130) or the A. nidulans homocysteine thiolactone hydrolase encoding gene blhA (AN6399) ( A. fumigatus genome does not encode any orthologue) (see Fig.S1G ). However, despite a strong overexpression of the...”
- Effects of amino acids on the lignocellulose degradation by Aspergillus fumigatus Z5: insights into performance, transcriptional, and proteomic profiles
Miao, Biotechnology for biofuels 2019 - “...enter the TCA cycle for energy production, during which the S -adenosylmethionine synthetase (AFUA_1G10630), adenosylhomocysteinase (AFUA_1G10130), cystathionine gamma-lyase (AFUA_8G04340), cystathionine beta-lyase MetG (AFUA_4G03950), cystathionine gamma-synthase (AFUA_7G01590), aspartate transaminase (AFUA_2G09650), and aspartate aminotransferase (AFUA_4G10410) were upregulated 1.11.7 times. Pyruvate decarboxylase PdcA (AFUA_3G11070), pyruvate decarboxylase (AFUA_6G00750), pyruvate dehydrogenase...”
- The Transcription Factor ZafA Regulates the Homeostatic and Adaptive Response to Zinc Starvation in Aspergillus fumigatus
Vicentefranqueira, Genes 2018 - “...AFUA_1G10060 NZR - 3.1 0.2 1.2 0.1 AFUA_1G10080 DZR zafA 31.5 3.0 21582 4316 Induction AFUA_1G10130 NZR sahA 1.9 0.1 1.1 0.1 AFUA_1G12830 IZR niaD 1.6 0.1 5.2 0.7 AFUA_1G13510 NZR facB 4.6 0.6 1.0 0.1 AFUA_1G14550 DZR sodC / sod3 1698.7 198.2 1176.5 142.4 Induction...”
- RNAseq analysis of Aspergillus fumigatus in blood reveals a just wait and see resting stage behavior
Irmer, BMC genomics 2015 - “...3.75 AFUA_5G14410 cysteine dioxygenase 5.96 7.05 AFUA_7G05660 translation elongation factor eEF-3 2.36 AFUA_5G05590 aspartokinase 2.37 AFUA_1G10130 adenosylhomocysteinase 2.09 AFUA_5G11340 translation initiation factor eIF-2B alpha 2.01 AFUA_8G05960 integral membrane protein 2.13 AFUA_2G05720 hypothetical protein 2.14 AFUA_5G02300 peroxidase 2.16 AFUA_8G01630 pyridine nucleotide-disulphide oxidoreductase 2.38 AFUA_4G00750 hypothetical protein 2.46...”
- The proteomic signature of Aspergillus fumigatus during early development
Cagas, Molecular & cellular proteomics : MCP 2011 - “...AFUA_6G10660 AFUA_6G02470 AFUA_1G06960 AFUA_2G16400 AFUA_1G10130 AFUA_5G07300 AFUA_1G13710 AFUA_1G05500 AFUA_1G02070 AFUA_2G04310 AFUA_6G04570 AFUA_4G09140 ORF...”
- Integrative analysis of the heat shock response in Aspergillus fumigatus
Albrecht, BMC genomics 2010 - “...1.03 Yes AFUA_6G10650 (ATP citrate lyase, subunit 1) 1.38 0.92 -0.16 0.33 Yes Nitrogen metabolism AFUA_1G10130 (adenosylhomocysteinase) 1.88 1.46 0.05 0.60 Yes AFUA_1G12840 (nitrite reductase NiiA) 2.75 2.30 1.19 1.54 Yes AFUA_2G11260 (3-isopropylmalate dehydratase) 1.18 0.90 -0.09 0.44 Yes AFUA_4G07360 (cobalamin-independent methionine synthase MetH/D) 1.53 0.66...”
- “...for an AP-2alphaA-like transcription factor in 11 of the 64 differentially regulated proteins (AFUA_1G05610, AFUA_4G07710, AFUA_1G10130, AFUA_5G04170, AFUA_5G07340, AFUA_2G17110, AFUA_2G10660, AFUA_3G09320, AFUA_6G12660, AFUA_1G02030, AFUA_7G01860; see Figure 4 for motif logo). The AP-2 family of transcription factors regulates proliferation and differentiation during embryonic development in animals [...”
- The Aspergillus fumigatus transcriptional regulator AfYap1 represents the major regulator for defense against reactive oxygen intermediates but is dispensable for pathogenicity in an intranasal mouse infection model
Lessing, Eukaryotic cell 2007 - “...synthetase (AFUA_4G13120) Carbamoylphosphate synthase (AFUA_5G06780) Adenosylhomocysteinase (AFUA_1G10130) 2.0 2.0 1.2 2.2 1.0 1.9 20 21 22 Cytoskeleton Actin...”
Q3MHL4 Adenosylhomocysteinase from Bos taurus
57% identity, 97% coverage
- The in vitro gastrointestinal digestion-associated protein corona of polystyrene nano- and microplastics increases their uptake by human THP-1-derived macrophages
Brouwer, Particle and fibre toxicology 2024 - “...specific PS MNP corona Condition Unique differentially abundant proteins Serum-coated PS 100 F1N045 (Complement C7) Q3MHL4 (Adenosylhomocysteinase) F1MB08 (phosphopyruvate hydratase) F1MW44(coagulation factor 13 chain) Q2HJ57 (Coactosin like protein) Q27967 (Secreted phosphoprotein 24) Q2KJ83 (carboxypeptidase N-catalytic chain) G3MZI7 (Collagen type V alpha chain) P80012 (von willebrand factor)...”
- Comparative Proteomic Analysis of Milk-Derived Extracellular Vesicles from Dairy Cows with Clinical and Subclinical Mastitis
Wang, Animals : an open access journal from MDPI 2023 - “...E1BHY6 GRN Granulin precursor 2.07 0.032 0.432 A0A3Q1LW07 CD46 Membrane cofactor protein 2.49 0.000 0.063 Q3MHL4 AHCY Adenosylhomocysteinase 3.28 0.009 0.311 animals-13-00171-t003_Table 3 Table 3 Reactome pathways analysis of DE proteins in milk-derive EVs between the CM and Healthy group. Pathway Name Entities Reactions Found Total...”
- Proteins from toad's parotoid macroglands: do they play a role in gland functioning and chemical defence?
Kowalski, Frontiers in zoology 2023 - “...A Apoptosis 5 P29117 44 22,072 2 8 Peptidyl-prolyl cistrans isomerase F, mitochondrial Apoptosis 5 Q3MHL4 153 48,021 4 6 Adenosylhomocysteinase Chronic inflammatory response, response to hypoxia 9, 10 Q4R5L1 148 46,548 2 3 Aspartate aminotransferase, cytoplasmic Biosynthesis of -glutamate 7 Q4PLJ0 144 9066 5 17...”
- Post-Translational Protein Deimination Signatures in Serum and Serum-Extracellular Vesicles of Bos taurus Reveal Immune, Anti-Pathogenic, Anti-Viral, Metabolic and Cancer-Related Pathways for Deimination
Criscitiello, International journal of molecular sciences 2020 - “...3 (METTL3)) F1MX80 1 35 Uncharacterised protein (SPAG9Sperm associated antigen 9) F1MZ69 1 35 Adenosylhomocysteinase Q3MHL4 1 35 Uncharacterised protein (HIVEP3Human immunodeficiency virus type I enhancer binding protein 3) F1MBK6 1 34 Coagulation factor V F1N0I3 1 33 Uncharacterised protein (VPREB1Uncharacterised protein) F1N160 1 33 Uncharacterised...”
- Proteomic changes in the milk of water buffaloes (Bubalus bubalis) with subclinical mastitis due to intramammary infection by Staphylococcus aureus and by non-aureus staphylococci
Pisanu, Scientific reports 2019 - “...HSP 90-alpha 1.88 2.06 1.68 PD Q3SZ54 Eukaryotic initiation factor 4A-I 1.85 1.82 1.88 GE Q3MHL4 Adenosylhomocysteinase 1.83 1.82 1.84 AM P80724 Brain acid soluble protein 1* 1.82 1.61 2 GE P60712 Actin, cytoplasmic 1 1.8 2.01 1.57 S J9Q6V1 Glutathione peroxidase* 1.8 2.05 1.5 OM...”
A0A452G990 Adenosylhomocysteinase from Capra hircus
57% identity, 97% coverage
CND00240 adenosylhomocysteinase from Cryptococcus neoformans var. neoformans JEC21
CNBD5970 hypothetical protein from Cryptococcus neoformans var. neoformans B-3501A
57% identity, 98% coverage
CNBG_6075 adenosylhomocysteinase from Cryptococcus deuterogattii R265
57% identity, 98% coverage
- Zap1 regulates zinc homeostasis and modulates virulence in Cryptococcus gattii
Schneider, PloS one 2012 - “...metabolism CNBG_3964 flavohemoglobin Oxidoreductase NAD-binding domain 2.24 Sulfate metabolism CNBG_1788 glutathione S-transferase Mak16 protein 2.08 CNBG_6075 S-adenosylhomocysteine hydrolase S-adenosyl-L-homocysteine hydrolase 2.00 CNBG_6043 glutathione transferase N/A 1.87 CNBG_1291 cystathionine beta-synthase Pyridoxal-phosphate dependent enzyme 4.34 Zinc-binding C CNBG_1642 transcription factor IIIb TFIIB zinc-binding; Brf1-like TBP-binding domain 3.67 CNBG_2093...”
Q4Q124 Adenosylhomocysteinase from Leishmania major
LMJF_36_3910 S-adenosylhomocysteine hydrolase from Leishmania major strain Friedlin
54% identity, 98% coverage
- Identification of protein profile in metacyclic and amastigote-like stages of Leishmania tropica: a proteomic approach
Ashrafmansouri, AMB Express 2022 - “...LMJF_35_3790 VLNAVIIR, ISTHAPAIV, NLYVISVK 36.1 Q4Q090 2,3-bisphosphoglycerate-independent phosphoglycerate mutase PGAM VALQGASLVDDALK, MFVTMDR, SAEITEAAIEALK, VALQGASLVDDALK 2.64 Q4Q124 Adenosylhomocysteinase LMJF_36_3910 AGVFFLPK, VAALHLAHVGAK, DISLAEWGR, EHVEIKPQVDR, VKDISLAEWGR, FDNLYGCR 7.9 Q4Q1D2 40S ribosomal protein S24 S24E-2 TTGFGLIYDDLASLK 2.8 Q4Q1F5 Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex LTITPIPMPALSPTMEK, WFQHFHDAMENPLSLLL 2.49 Q4Q1X7 Putative...”
- S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling
Miller, Journal of bacteriology 2015 - “...Aquifex aeolicus; P9WGV3, Mycobacterium tuberculosis (PDB 2ZIZ); Q4Q124, Leishmania major (PDB 3G1U); and P23526, Homo sapiens (PDB 3NJ4). Determination of the...”
- Identification of protein profile in metacyclic and amastigote-like stages of Leishmania tropica: a proteomic approach
Ashrafmansouri, AMB Express 2022 - “...ISTHAPAIV, NLYVISVK 36.1 Q4Q090 2,3-bisphosphoglycerate-independent phosphoglycerate mutase PGAM VALQGASLVDDALK, MFVTMDR, SAEITEAAIEALK, VALQGASLVDDALK 2.64 Q4Q124 Adenosylhomocysteinase LMJF_36_3910 AGVFFLPK, VAALHLAHVGAK, DISLAEWGR, EHVEIKPQVDR, VKDISLAEWGR, FDNLYGCR 7.9 Q4Q1D2 40S ribosomal protein S24 S24E-2 TTGFGLIYDDLASLK 2.8 Q4Q1F5 Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex LTITPIPMPALSPTMEK, WFQHFHDAMENPLSLLL 2.49 Q4Q1X7 Putative 40S ribosomal...”
Q710C4 Adenosylhomocysteinase from Sus scrofa
57% identity, 97% coverage
Ahcy / P10760 S-adenosylhomocysteine hydrolase subunit (EC 3.13.2.1) from Rattus norvegicus (see paper)
SAHH_RAT / P10760 Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Rattus norvegicus (Rat) (see 4 papers)
P10760 adenosylhomocysteinase (EC 3.3.1.1) from Rattus norvegicus (see 2 papers)
NP_058897 adenosylhomocysteinase from Rattus norvegicus
57% identity, 97% coverage
- function: Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed:11927587). Binds copper ions (By similarity).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer. Interaction with AHCYL1 (By similarity). - Candidate proteins interacting with cytoskeleton in cells from the basal airway epithelium in vitro
Olatona, Frontiers in molecular biosciences 2024 - “...105.4 0.016 P85968 Pgd 6-phosphogluconate dehydrogenase, decarboxylating 53.2 0.156 Q9JMI1 Aacs Acetoacetyl-CoA synthetase 87.6 0.175 P10760 Ahcy Adenosylhomocysteinase 47.5 0.065 P51635 Akr1a1 Aldo-keto reductase family 1 member A1 36.5 0.040 P15178 Dars1 Aspartate--tRNA ligase, cytoplasmic 57.1 0.129 P16638 Acly ATP-citrate synthase 120.6 0.723 P22734 Comt Catechol...”
- Urinary protein changes during the short-term growth and development of rats.
Liu, Heliyon 2024 - “...Putative lysozyme C-2 2.23 2.48E-04 P46413 Glutathione synthetase 0.39 2.61E-04 F1M498 Gastrokine 3 2.55 2.83E-04 P10760 Adenosylhomocysteinase 0.48 2.85E-04 Q63355 Unconventional myosin-Ic 0.43 3.37E-04 G3V7D0 Matrix metallopeptidase 8 2.39 3.70E-04 P23928 Alpha-crystallin B chain 0.39 3.76E-04 Q6JE36 Protein NDRG1 2.03 6.23E-04 M0R8R4 Oncoprotein-induced transcript 3 protein...”
- Early urinary candidate biomarkers and clinical outcomes of intervention in a rat model of experimental autoimmune encephalomyelitis
Zhao, Royal Society open science 2023 - “...3.3510 2 other P02631 oncomodulin 12 yes 0.61 2.6410 3 other tissue [ 29 ] P10760 adenosylhomocysteinase 48 yes 0.60 1.8510 2 enzyme P11232 thioredoxin 12 yes 0.60 2.0810 3 enzyme serum [ 30 ] O88989 malate dehydrogenase 1 36 yes 0.59 7.1210 3 enzyme Q9QZ76...”
- Identification of proteins regulated by chlorogenic acid in an ischemic animal model: a proteomic approach.
Shah, Laboratory animal research 2023 - “...46 9 Protein phosphatase 2A, regulatory subunit B P58389 36.59 5.88 9/56 29 10 Adenosylhomocysteinase P10760 47.55 6.07 15/132 33 11 Thioredoxin Q920J4 32.23 4.84 8/87 42 12 Mu-crystallin Q9QYU4 33.53 5.34 9/86 24 13 Mu-crystallin Q9QYU4 33.55 5.30 6/114 21 14 Mu-crystallin Q9QYU4 33.53 5.34...”
- Proteome Analysis of Urinary Biomarkers in a Bovine IRBP-Induced Uveitis Rat Model via Data-Independent Acquisition and Parallel Reaction Monitoring Proteomics
Qin, Frontiers in molecular biosciences 2022 - “...Q6AYA5 Transmembrane protein 106B 1.51 P07171 Calbindin 0.62 Q4FZU2 Keratin, type II cytoskeletal 6A 0.59 P10760 Adenosylhomocysteinase 0.56 The overlap of these 76 differential proteins identified in EAU rats is shown with a Venn diagram ( Figure 3 ). Fifteen urinary proteins changed significantly both on...”
- Identification of changed proteins by retinoic acid in cerebral ischemic damage: a proteomic study.
Kang, The Journal of veterinary medical science 2022 - “...5.91 19/45 41 ** 8 Unknown ** 9 Unknown ** 10 Unknown ** 11 Adenosylhomocysteinase P10760 47.53 6.07 15/132 33 *** 12 Isocitrate dehydrogenase [NAD + ] subunit Q99NA5 39.58 6.47 8/93 31 ** 13 Rab GDP dissociation inhibitor P50399 50.50 5.90 9/150 23 *** 14...”
- Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.
Camino, International journal of molecular sciences 2022 - “...P70580 Membrane-associated progesterone receptor component 1 0.032 2.08 FUCO_RAT P17164 Tissue alpha-L-fucosidase 0.004 2.07 SAHH_RAT P10760 Adenosylhomocysteinase 0.053 2.04 ATPB_RAT P10719 ATP synthase subunit beta, mitochondrial 0.053 2.03 PUR9_RAT O35567 Bifunctional purine biosynthesis protein ATIC 0.043 1.98 Elevated in Obese VAT EVs NHRF2_RAT Q920G2 Na(+)/H(+) exchange...”
- iTRAQ-based quantitative proteomics analysis of the hepatoprotective effect of melatonin on ANIT-induced cholestasis in rats.
Wang, Experimental and therapeutic medicine 2021 - “...1.6969 0.0045 P09118 Urate oxidase (UOX) 2.1557 0.0020 P09811 Phosphorylase, glycogen, liver (PYGl) 2.1289 0.0647 P10760 Adenosylhomocysteinase (AHCY) 2.1517 0.0252 P12785 Fatty acid synthase (FASN) 1.9435 0.0444 P14141 Carbonic anhydrase 3 (CAR3) 1.5513 0.0001 P14173 Dopa decarboxylase (DDC) 1.6658 0.0224 P15083 Polymeric immunoglobulin receptor (PIGR) 0.6010...”
- More
- S-adenosyl homocysteine hydrolase is required for Myc-induced mRNA cap methylation, protein synthesis, and cell proliferation.
Fernandez-Sanchez, Molecular and cellular biology 2009 - GeneRIF: report that Myc promotes upregulation of S-adenosyl homocysteine hydrolase.
- Expression and localization of S-adenosylhomocysteine-hydrolase in the rat kidney following carbon monoxide induced hypoxia.
Kloor, Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2007 (PubMed)- GeneRIF: AdoHcy-hydrolase accumulation in the nucleus of adult mammalian cells is involved in maintaining efficient transmethylation reactions in transcriptionally active cells by removing the product inhibitor AdoHcy.
- Inhibition of S-adenosylhomocysteine hydrolase by acyclic sugar adenosine analogue D-eritadenine. Crystal structure of S-adenosylhomocysteine hydrolase complexed with D-eritadenine.
Huang, The Journal of biological chemistry 2002 (PubMed)- GeneRIF: Crystal structures of DEA alone and in complex with AdoHcyase have been determined to elucidate the DEA binding scheme to AdoHcyase
- Catalytic mechanism of S-adenosylhomocysteine hydrolase. Site-directed mutagenesis of Asp-130, Lys-185, Asp-189, and Asn-190.
Takata, The Journal of biological chemistry 2002 (PubMed)- GeneRIF: Catalytic mechanism of S-adenosylhomocysteine hydrolase
XP_658867 adenosylhomocysteinase from Aspergillus nidulans FGSC A4
56% identity, 97% coverage
- Protein profiling of the dimorphic, pathogenic fungus, Penicillium marneffei
Chandler, Proteome science 2008 - “...+ 2 OM K.AKDGAGSATLSMAMAGAR.M + 2 OM K.VELGPNGVEKILPVGQVNAYEEK.L 25 C7 (-) hypothetical protein (S-adenosyl-L-homocysteine hydrolase) XP_658867 (gi|67521612) 6.02/49.3 5.7/60.5 Aspergillus nidulans 161 4/5 R.ATDVMIAGK.V + OM K.VAVVAGFGDVGK.G K.VADISLAAFGR.R K.SVQNIKPQVDR.Y 9 C18 (3) proteasome component Pre9 XP_750785 (gi|70991873) 5.80/28.5 5.7/49.1 Aspergillus fumigatus 165 4/5 K.IEFATVGK.T R.TTIFSPEGR.L K.LLEQDTSAEK.L...”
O13639 Adenosylhomocysteinase from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
SPBC8D2.18c adenosylhomocysteinase (predicted) from Schizosaccharomyces pombe
56% identity, 98% coverage
- Effect of Amino Acids on Fusarium oxysporum Growth and Pathogenicity Regulated by TORC1-Tap42 Gene and Related Interaction Protein Analysis.
Deng, Foods (Basel, Switzerland) 2023 - “...FBA2 Fructose-bisphosphate aldolase 2 39,973 126 Q5AWS6 cdc48 Cell division control protein 48 90,769 124 O13639 pi047 Adenosylhomocysteinase 47,866 121 Q7RVA8 ace-8 Pyruvate kinase 58,290 115 P49382 AAC ADP, ATP carrier protein 33,187 111 Q12629 PDC1 Pyruvate decarboxylase 61,905 110 Q6C1F3 ENO Enolase 47,277 108 Q9HES8...”
- Global network analysis in Schizosaccharomyces pombe reveals three distinct consequences of the common 1-kb deletion causing juvenile CLN3 disease
Minnis, Scientific reports 2021 - “...kms1, cds1 ), neurological ( hhp2, SPCC1827.07c ), and inborn mitochondrial metabolomic ( cyt1, fsf1, SPBC8D2.18c ) disorders. This suggests a shared compensatory network between these varied disorders and CLN3 disease (Table 5 ). Table 4 Summary of human diseases and their corresponding yeast genes for...”
- S-Adenosylmethionine Synthetase Is Required for Cell Growth, Maintenance of G0 Phase, and Termination of Quiescence in Fission Yeast
Hayashi, iScience 2018 - “...Schematic illustration of the methionine cycle in the fission yeast, S.pombe . Sam1, SAM synthetase; SPBC8D2.18c, adenosylhomocysteinase (AHCY); Met26, methionine synthase. (B) Locations of mutations of sam1 mutants determined by nucleotide sequencing in domain architecture based on the three-dimensional structure. (C) sam1-1sam1-5 mutants failed to produce...”
- Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach
García-Santamarina, Antioxidants & redox signaling 2013 - “...Cellular amino acid metabolic process SPBC8D2.18c adenosylhomocysteinase 196 Leu2 3-isopropylmalate dehydratase 437 SPAC9E9.06c threonine synthase 490...”
- Involvement of S-adenosylmethionine in G1 cell-cycle regulation in Saccharomyces cerevisiae
Mizunuma, Proceedings of the National Academy of Sciences of the United States of America 2004 - “...showed similarity to those of Schizosaccharomyces pombe SPBC8D2.18c, Drosophila melanogaster Ahcy13, and human AHCY, suggesting that it encodes a protein...”
- Autoregulated expression of Schizosaccharomyces pombe meiosis-specific transcription factor Mei4 and a genome-wide search for its target genes
Abe, Genetics 2000 - “...mde1 (SPAC16E8.05c) mde2 (SPBC31F10.08) mde3 (SPBC8D2.18c) mde4 (SPBC6B1.04) mde5 (SPAC4A8.01) mde6 (SPAC15A10.10) mde7 (SPCC320.07c) mde8 (SPBC19F8.01c)...”
A0JCJ9 Adenosylhomocysteinase from Plutella xylostella
55% identity, 97% coverage
AHCY / P23526 Adenosylhomocysteinase (EC 3.13.2.1) from Homo sapiens (see 3 papers)
SAHH_HUMAN / P23526 Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Homo sapiens (Human) (see 13 papers)
P23526 adenosylhomocysteinase (EC 3.3.1.1) from Homo sapiens (see 15 papers)
NP_000678 adenosylhomocysteinase isoform 1 from Homo sapiens
56% identity, 97% coverage
- function: Catalyzes the hydrolysis of S-adenosyl-L-homocysteine to form adenosine and homocysteine (PubMed:10933798). Binds copper ions (By similarity).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer (PubMed:19177456, PubMed:28647132, PubMed:9586999). Interaction with AHCYL1 (PubMed:28647132). - Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...adult tissues ( Chen et al., 2010 ) and encodes three annotated splicing isoforms (AHCY1 (NP_000678), 2 (NP_001155238), and 3 (NP_001309015), with 432, 404, and 434 amino acids, respectively) that differ only in their first 30 amino acids. These isoforms have not been formally compared side-by-side,...”
- Vorinostat impairs the cancer-driving potential of leukemia-secreted extracellular vesicles
Massaro, Journal of translational medicine 2025 - “...ACTR3 0 167705 #DIV/0! 3 3 15,3 15,3 15,3 5,3174 7043500 5 0 0 0 P23526 Adenosylhomocysteinase AHCY 0 82303,66667 #DIV/0! 2 2 6,9 6,9 6,9 5,2467 4938200 4 0 0 0 Q9UKK9 ADP-sugar pyrophosphatase NUDT5 0 330765 #DIV/0! 4 4 22,8 22,8 22,8 8,7096 7276900...”
- “...YWHAG P61247 RPS3A P62081 RPS7 P49189 ALDH9A1 P10809 HSPD1 P27635 RPL10 P30050 RPL12 P61158 ACTR3 P23526 AHCY Q9UKK9 NUDT5 P30838 ALDH3A1 A8K2U0 A2ML1 O43707 ACTN4 P09525 ANXA4 P08758 ANXA5 P20073 ANXA7 O15523 P07686 HEXB Q9UI42 CPA4 P07858 CTSB O60911 CTSV P60953 CDC42 Q00610 CLTC P35606 COPB2...”
- mTORC1-Driven Protein Translation Correlates with Clinical Benefit of Capivasertib within a Genetically Preselected Cohort of PIK3CA-Altered Tumors.
Sobsey, Cancer research communications 2024 - “...Protein name EIF4EBP1 (p) 4E-BP1 Q13541 Eukaryotic translation initiation factor 4E-binding protein 1 AHCY AdoHcyase P23526 Adenosylhomocysteinase AKT1 (p)AKT1 P31749 RAC-alpha serine/threonine-protein kinase AKT2 (p)AKT2 P31751 RAC-beta serine/threonine-protein kinase ALB Albumin P02768 Albumin AMPK AMPK -2 P54646 5-AMP-activated protein kinase catalytic subunit alpha-2 APRT APRT P07741...”
- Cytoskeleton Remodeling-Related Proteins Represent a Specific Salivary Signature in PSC Patients.
Ceccherini, Molecules (Basel, Switzerland) 2024 - “...Tyrosine-protein phosphatase non-receptor type 6 1.968 0.008 P49368 T-complex protein 1 subunit gamma 1.714 0.002 P23526 Adenosylhomocysteinase 1.543 0.005 Q96BQ1 Protein FAM3D 1.503 0.005 P19823 Inter-alpha-trypsin inhibitor heavy chain H2 1.464 0.015 P23528 Cofilin-1 1.413 0.001 Q08431 Lactadherin 1.358 0.016 P14550 Aldo-keto reductase family 1 member...”
- ARID3C Acts as a Regulator of Monocyte-to-Macrophage Differentiation Interacting with NPM1.
Kim, Journal of proteome research 2024 - “...122 23.0243 22.6641 NaN NaN 2 6 P02790 123 23.8524 23.8998 NaN NaN 3 6 P23526 124 22.6404 22.507 NaN NaN 2 6 P61158 125 23.684 24.0302 NaN NaN 2 6 Q15843 126 23.1316 23.1095 NaN NaN 3 5 O15144 127 22.9613 22.9744 NaN NaN 3...”
- MSC secretome from amniotic fluid halts IL-1β and TNF-α inflammation via the ERK/MAPK pathway, promoting cartilage regeneration in OA in vitro.
Klaymook, Journal of stem cells & regenerative medicine 2024 - Copper Homeostasis in the Model Organism C. elegans.
Ohse, Cells 2024 - “...Reference e AFP P02771 no ortholog [a], [b] ALB P02768 no ortholog [a], [b] AHCY P23526 ahcy-1 [a], [b] APP P05067 apl-1 * [ 14 , 74 ] APLP2 Q06481 CUTA O60888 F35G12.7 [a], [b] F5 P12259 ddr-1 [b] GPC1 P35052 gpn-1 [a], [b] LTF P02788...”
- The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores
Burigotto, 2023 - N-terminal proteoforms may engage in different protein complexes
Bogaert, Life science alliance 2023 - “...Q12933 TNF receptorassociated factor 2 Yes Yes Yes ADK P55263 Adenosine kinase Yes Yes AHCY P23526 Adenosylhomocysteinase Yes Yes ASNA1 O43681 ATPase ASNA1 Yes Yes ASS1 P00966 Argininosuccinate synthase Yes Yes BAG6 P46379 Large proline-rich protein BAG6 Yes Yes Yes BROX Q5VW32 BRO1 domaincontaining protein BROX...”
- More
V3YXF9 Adenosylhomocysteinase from Lottia gigantea
LOTGIDRAFT_184532 hypothetical protein from Lottia gigantea
55% identity, 97% coverage
1a7aA / P23526 Structure of human placental s-adenosylhomocysteine hydrolase: determination of a 30 selenium atom substructure from data at a single wavelength (see paper)
57% identity, 97% coverage
- Ligands: nicotinamide-adenine-dinucleotide; (1'r,2's)-9-(2-hydroxy-3'-keto-cyclopenten-1-yl)adenine (1a7aA)
PAAG_02859 adenosylhomocysteinase from Paracoccidioides lutzii Pb01
55% identity, 97% coverage
- Differential Metabolism of a Two-Carbon Substrate by Members of the Paracoccidioides Genus
Baeza, Frontiers in microbiology 2017 - “...01 from the degradation of methionine and threonine because of the induction of adenosyl homocysteinase (PAAG_02859) and threonine dehydratase (PAAG_03168) (Figure 7 and Supplementary Figure 5 ). Glyoxylate production from the degradation of glycine was observed in Pb 01, Pb 03, and Pb EPM83 with the...”
- Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone
Prado, Frontiers in microbiology 2015 - “...0.691 GDP-mannose pyrophosphorylase A PAAG_08174 860.91 * Transketolase TktA PAAG_04444 2581.21 0.763 Coenzyme metabolism Adenosylhomocysteinase PAAG_02859 14585.17 0.440 Dihydropteroate synthase PAAG_01324 870.83 0.779 Pyridoxine biosynthesis protein pyroA PAAG_07321 2354.97 0.787 S-adenosylmethionine synthase PAAG_02901 6069.65 0.357 Nucleotide metabolism Bifunctional purine biosynthesis protein ADE17 PAAG_00731 4517.89 0.811 Adenylosuccinate...”
PADG_01886 adenosylhomocysteinase from Paracoccidioides brasiliensis Pb18
55% identity, 97% coverage
3h9uC / Q383X0 S-adenosyl homocysteine hydrolase (sahh) from trypanosoma brucei
55% identity, 97% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (3h9uC)
Q6WN55 adenosylhomocysteinase (EC 3.3.1.1) from Branchiostoma belcheri tsingtauense (see paper)
55% identity, 97% coverage
L8I9Z4 Adenosylhomocysteinase (Fragment) from Bos mutus
57% identity, 97% coverage
W5PIJ2 Adenosylhomocysteinase from Ovis aries
57% identity, 97% coverage
B7P4Y1 Adenosylhomocysteinase from Ixodes scapularis
55% identity, 100% coverage
SAHH_CANAL / P83783 Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) (see paper)
55% identity, 99% coverage
- function: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine.
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
NP_001309015 adenosylhomocysteinase isoform 3 from Homo sapiens
56% identity, 97% coverage
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...al., 2010 ) and encodes three annotated splicing isoforms (AHCY1 (NP_000678), 2 (NP_001155238), and 3 (NP_001309015), with 432, 404, and 434 amino acids, respectively) that differ only in their first 30 amino acids. These isoforms have not been formally compared side-by-side, although ectopically expressed hAHCY1 mutant...”
6gbnB Crystal structure of s-adenosyl-l-homocysteine hydrolase from cytophaga hutchinsonii in complex with adenosine
53% identity, 98% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (6gbnB)
SCO3023 S-adenosyl-L-homocysteine hydrolase from Streptomyces coelicolor A3(2)
51% identity, 94% coverage
bglu_1g01990 S-adenosyl-L-homocysteine hydrolase from Burkholderia glumae BGR1
52% identity, 99% coverage
- Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae
Kang, Scientific reports 2019 - “...::) and BGS9 (BGR1 qsmR ::), indicated that QS might regulate the expression of ahcY (bglu_1g01990), we performed qRT-PCR analysis of ahcY in BGR1, BGS2, and BGS9 to confirm these results. Expression of ahcY was approximately 23 times higher in the wild type than in the...”
- “.... glumae BGR1. Schematic organization of the ahcY locus: 1, bglu_1g01970; 2, bglu_1g01980; ahcY , bglu_1g01990; pmp1 , bglu_1g02000; metF , bglu_1g02010; 3, bglu_1g02020; 4, bglu_1g02030 (GenBank accession numbers: CP001503CP001508). Arrows with arrowheads represent the direction and length of cDNA. Vertical bars in the map denote...”
T551_03089 adenosylhomocysteinase from Pneumocystis jirovecii RU7
55% identity, 97% coverage
Rru_A3444 Adenosylhomocysteinase from Rhodospirillum rubrum ATCC 11170
54% identity, 98% coverage
FPSE_06206 hypothetical protein from Fusarium pseudograminearum CS3096
55% identity, 97% coverage
NP_010961 adenosylhomocysteinase from Saccharomyces cerevisiae S288C
P39954 Adenosylhomocysteinase from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
YER043C S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor from Saccharomyces cerevisiae
55% identity, 98% coverage
- S-adenosyl-L-homocysteine hydrolase, key enzyme of methylation metabolism, regulates phosphatidylcholine synthesis and triacylglycerol homeostasis in yeast: implications for homocysteine as a risk factor of atherosclerosis.
Malanovic, The Journal of biological chemistry 2008 - GeneRIF: cells with down-regulated Sah1 activity major alterations in the expression of the INO1 gene as well as in the localization of Opi1, a negative regulatory factor of phospholipid synthesis
- Using quantitative redox proteomics to dissect the yeast redoxome
Brandes, The Journal of biological chemistry 2011 - “...65 13 19 4 45 7 49 5 P13663 P39954 P37292 P00931 Carbohydrate metabolism P00330 Q04894 P14540 P09624 P06169 P00358 P32614 P11986 Proteostasis and stress...”
- “...16 Supplemental Information: Brandes, Reichmann et al. Q12464 P39954 P39954 P10659 P10080 P21243 Q00711 P47052 P29478 P33330 P07284 P07284 P07284 Q12118 P37292...”
- In planta proteomics and proteogenomics of the biotrophic barley fungal pathogen Blumeria graminis f. sp. hordei.
Bindschedler, Molecular & cellular proteomics : MCP 2009 (no snippet) - Precise control of SCRaMbLE in synthetic haploid and diploid yeast
Jia, Nature communications 2018 - “...the DNA copy numbers of four genes ( YEL071W , CrtE , YEL022W , and YER043C ) that were representative of the duplicated segments (Fig. 4e ). For yJBS001, the fold change in the copy numbers of the YEL072W-YEL071W , YEL070W-YEL060C , YEL027W-YEL022W , and YER043C-YER044W...”
- “...used for qPCR analysis. The ALG9 gene and the YEL071W , CrtE , YEL022W , YER043C , and YER036C genes were chosen as the reference gene and target genes, respectively. The copy numbers were quantified by comparing the Cq values of the target genes and the...”
- Transcriptional profiling reveals molecular basis and novel genetic targets for improved resistance to multiple fermentation inhibitors in Saccharomyces cerevisiae
Chen, Biotechnology for biofuels 2016 - “...YPL053C Cellular amino acid metabolic process (GO:0006520) YBL098W, YDL168W, YDL182W, YDR019C, YDR135C, YDR502C , YER026C, YER043C, YER091C, YGL256W , YGR209C, YIL074C, YJL101C, YJR010W, YJR137C, YJR139C, YKL001C, YKR080W, YLR058C, YLR142W , YLR180W, YLR231C, YLR303W, YMR169C , YMR189W, YMR300C, YNL135C, YNL160W, YOL058W, YOL086C, YOR184W, YOR375C YCL030C, YDR007W, YDR502C...”
- “...YLR142W , YMR169C , YMR250W YCL030C, YCR005C, YDL168W, YDL182W, YDR007W, YDR135C, YDR502C , YDR513W, YEL046C, YER043C, YER091C , YFL030W, YGL009C, YGL256W , YGR209C, YGR267C, YIL074C, YJL101C, YJR010W, YJR137C, YJR139C, YKL001C, YKR080W, YLR058C, YLR142W , YLR180W, YLR303W, YMR169C , YMR189W, YNL160W, YOL064C, YPL091W Lipid metabolic process (GO:0006629)...”
- Comparative proteome analysis of Saccharomyces cerevisiae: a global overview of in vivo targets of the yeast activator protein 1
Jun, BMC genomics 2012 - “...- Protein involved in pathways other than carbon metabolism Amino-acid and nucleotide metabolism Sah1p Adenosylhomocysteinase YER043C 631 143 1310 304 2.08 0.025 - Shm2p Serine hydroxymethyltransferase YLR058C 486 26 944 176 1.94 0.011 - Aro9p Aromatic amino acid aminotransferase 2 YHR137W 116 19 164 13 1.41...”
- Deletion of a subgroup of ribosome-related genes minimizes hypoxia-induced changes and confers hypoxia tolerance
Shah, Physiological genomics 2011 - “...YNL055C YDR032C YBR218C YNL098C YDL135C YBR049C YDL204W YER043C YBR214W YMR079W YJR134C YGL213C YLR178C YBR162C YPL203W YFR010W YEL021W YGR094W YOR230W YKL196C...”
- Comparative transcriptomic and proteomic profiling of industrial wine yeast strains
Rossouw, Applied and environmental microbiology 2010 - “...SAM2 SEC53 SER1 SER33 YGR180C YDL130W YER043C YLR180W YDR502C YFL045C YOR184W YIL074C Coproporphyrinogen III oxidase ATP phosphoribosyltransferase...”
- Involvement of S-adenosylmethionine in G1 cell-cycle regulation in Saccharomyces cerevisiae
Mizunuma, Proceedings of the National Academy of Sciences of the United States of America 2004 - “...growth phenotype was recovered. The plasmid contained the YER043c ORF encoding a polypeptide similar to the human enzyme involved in the hydrolysis of AdoHcy...”
- “...protein conserved among eukaryotes. Comparison of Scz7p and YER043c sequences revealed the presence of three amino acid substitutions, i.e., I247V, T279I, and...”
- Identification and functional reconstitution of yeast mitochondrial carrier for S-adenosylmethionine
Marobbio, The EMBO journal 2003 - “...The coding sequences for Sam5p (YNL003c) and Sah1p (YER043c) were ampli(R)ed by PCR from S.cerevisiae genomic DNA without termination codons and with additional...”
- Microarray analysis of orthologous genes: conservation of the translational machinery across species at the sequence and expression level
Jiménez, Genome biology 2003 - “...G G3 - COG0021 YPR074C TKL1 transketolase 1 - RRPE - H - - COG0499 YER043C SAH1 S-adenosyl-l-homocysteine hydrolase - - - H - - COG0192 YDR502C SAM2 S-adenosylmethionine synthetase 2 - - - H - - COG0192 YLR180W SAM1 S-adenosylmethionine synthetase 1 - RRPE PAC...”
- More
VDAG_07814 adenosylhomocysteinase from Verticillium dahliae VdLs.17
54% identity, 97% coverage
A4HPQ9 Adenosylhomocysteinase from Leishmania braziliensis
54% identity, 98% coverage
LINJ_36_4100 S-adenosylhomocysteine hydrolase from Leishmania infantum JPCM5
A4ID05 Adenosylhomocysteinase from Leishmania infantum
54% identity, 98% coverage
- Unravelling the proteomic signature of extracellular vesicles released by drug-resistant Leishmania infantum parasites
Douanne, PLoS neglected tropical diseases 2020 - “...MF200.5 AmB1000.1 LINJ_26_0150 60S ribosomal protein L7 putative 93.3 77.0 (0.83) 46.7 (0.50) 5.7 (0.06) LINJ_36_4100 Adenosyl homocysteinase 13.7 7.7 (0.56) 12.7 (0.93) 7.0 (0.51) LINJ_32_0410 ATP-dependent RNA helicase putative 95.7 49.0 (0.51) 89.7 (0.94) 9.7 (0.10) LINJ_17_0180 Elongation factor 1-alpha 75.3 65.7 (0.87) 115.7 (1.54)...”
- Potential selection of antimony and methotrexate cross-resistance in Leishmania infantum circulating strains
Bernardo, PLoS neglected tropical diseases 2024 - “...LINF 360031300 Methyltransferase 46.44 46.02 0.42 A4HRP2 LINF 020009800 Voltage-dependent anion-selective channel-putative 48.47 48.12 0.35 A4ID05 LINF 360048000 Adenosylhomocysteinase 51.22 50.91 0.31 A4I1R2 LINF 250028300 Succinate-CoA ligase [ADP-forming] subunit alpha 45.44 45.16 0.28 A4I120 LINF 240027300 3-hydroxy-3-methylglutaryl-CoA synthase- putative 48.54 48.27 0.27 A4HUX3 LINF 110012000 Aminopeptidaseputative...”
- Exploring direct and indirect targets of current antileishmanial drugs using a novel thermal proteomics profiling approach
Ibarra-Meneses, Frontiers in cellular and infection microbiology 2022 - “...heat-shock protein 83 (E9AHM9), a thiol-specific peroxidase involved in cellular oxidant detoxification (Q95U89), an adenosylhomocysteinase (A4ID05) responsible for the synthesis of L-homocysteine, and a putative N(alpha)-terminal acetyltransferase (A4HXH5). On the other hand, the 12 significant proteins found in the AmB1000.1 melting proteome included two ribosomal proteins...”
- “...protein S15 57.2 46.6 10.6 No 0 Q95U89 LINF_230005400 Peroxiredoxin 54.8 45.1 9.8 No 0 A4ID05 LINF_360048000 S-adenosylhomocysteine hydrolase 59.6 51.3 8.3 No 0 A4IE56 LINF_360050900 Oxidoreductase - putative 50.8 44.4 6.4 No 0 A4HYZ5 LINF_200021000 Putative 40S ribosomal protein S11 53.3 47.1 6.2 No 0...”
A4HPQ8 Adenosylhomocysteinase from Leishmania braziliensis
54% identity, 98% coverage
BPSL3290 adenosylhomocysteinase from Burkholderia pseudomallei K96243
Q3JY79 Adenosylhomocysteinase from Burkholderia pseudomallei (strain 1710b)
BP1026B_I3525 adenosylhomocysteinase from Burkholderia pseudomallei 1026b
52% identity, 99% coverage
- Altered Proteome of Burkholderia pseudomallei Colony Variants Induced by Exposure to Human Lung Epithelial Cells
Al-Maleki, PloS one 2015 - “...17 6 38.49/38.31 5.97/5.87 361 - Cytoplasmic -2.21 0.003 D. Coenzyme Metabolism 14 Adenosylhomocysteinase (AhcY) BPSL3290 24 9 52.17/52.07 5.73/5.73 500 - Cytoplasmic -3.70 0.00113 E. Secondary Metabolites Biosynthesis, Transport and Catabolism 16 Putative non-ribosomally encoded peptide/polyketide synthase (PhyH) BPSS1183 29 8 35.38/35.28 5.77/5.77 376 -...”
- “...17 6 38.49/38.31 5.97/5.87 361 - Cytoplasmic 3.20 0.02 D. Coenzyme Metabolism 14 Adenosylhomocysteinase (AhcY) BPSL3290 24 9 52.17/52.07 5.73/5.73 500 - Cytoplasmic 3.01 0.01 48 Phenylacetate-coenzyme A ligase (PaaA) BPSL3045 21 6 47.74 /47.51 5.80/5.80 289 - Cytoplasmic 2.56 0.04 E. Nucleotide Transport and Metabolism...”
- Combining crystallographic and binding affinity data towards a novel dataset of small molecule overlays.
Hönig, Journal of computer-aided molecular design 2024 - “...Identical Pfam IDs with multiple UniProt accession numbers can be due to differing organisms (e.g., Q3JY79 and Q9SP37 for cluster RAB_A_60-3glq), virus strains (e.g., C6KP13 and C3W5S3 for cluster LNV_B_801-3ti3 or proteins with identical binding sites (e.g., P45984, P53779 and P45983 for cluster 880_A_502-4z91). The reasons...”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...(O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by Clustal Omega ( https://www.ebi.ac.uk/Tools/msa/clustalo/ ) and Phylodendron ( http://iubioarchive.bio.net/treeapp/treeprint-form.html...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...of the following species (UniProt accession code): Lupinus luteus (Q9SP37), Plasmodium falciparum (P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode...”
- Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae
Kang, Scientific reports 2019 - “...(BTH_I0358) - metH2 (BTH_I0357) BTH_I0174 - BTH_II1307 - BTH_II1338 - BTH_I2673 Burkholderia pseudomallei 1026b c BP1026B_I3525 BP1026B_I3523 BP1026B_I0767 - metH1 (BP1026B_I3121) - metH2 (BP1026B_I3120) BP1026B_I3302 - BP1026B_I0156 - BP1026B_I0642 - BP1026B_I0822 - BP1026B_I2850 - BP1026B_II1162 Pseudomonas aeruginosa PAO1 d PA0432* PA0430* PA1927* PA1843* PA0546 - PA4209...”
BCAL0145 adenosylhomocysteinase from Burkholderia cenocepacia J2315
51% identity, 99% coverage
- Small Noncoding Regulatory RNAs from Pseudomonas aeruginosa and Burkholderia cepacia Complex
Pita, International journal of molecular sciences 2018 - “...located on genomic island BcenGI9; two conserved regulatory motifs; the SAH riboswitch located upstream of BCAL0145; an adenosylhomocysteinase; the sucA RNA motif located upstream of sucA (BCAL1515), an enzyme of the citric acid cycle; and four sRNAs from the family named toxic small RNAs, whose expression...”
- Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315
Sass, BMC genomics 2015 - “...the yet unannotated S-adenosyl-L-homocysteine (SAH) riboswitch [ 20 ] in the 5UTR of an adenosylhomocysteinase (BCAL0145, see below). Transcription initiation in genomic islands The genome of B. cenocepacia J2315 contains 14 genomic islands with a GC-content lower than genome average of 66.9% or with CDS similar...”
- “...Two Rfam hits constitute conserved regulatory motifs: the SAH riboswitch located in the 5UTRs of BCAL0145, an adenosylhomocysteinase, and the Burkholderiales -specific sucA RNA motif located in the 5UTR of BCAL1515, sucA , an enzyme of the citric acid cycle. The sucA RNA motif probably constitutes...”
- Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis
Sass, BMC genomics 2011 - “...+ 10/50 mg/L TMP/SMX J2315-T J2315-T + 10/50 mg/L TMP/SMX J2315-T + 60/300 mg/L TMP/SMX BCAL0145 Adenosylhomocysteinase 1.77 - - 2.26 BCAL0147 5,10-methylenetetrahydro-folate reductase 1.61 - - 2.07 BCAM0186 Lectin 2.90 - - - BCAM1869 Conserved hypothetical protein - -3.68 -4.13 -2.09 BCAM1870 N-acylhomoserine lactone synthase...”
P36889 Adenosylhomocysteinase from Leishmania donovani
54% identity, 98% coverage
3d64A / Q3JY79 Crystal structure of s-adenosyl-l-homocysteine hydrolase from burkholderia pseudomallei
53% identity, 98% coverage
- Ligand: nicotinamide-adenine-dinucleotide (3d64A)
BP3068 adenosylhomocysteinase from Bordetella pertussis Tohama I
54% identity, 99% coverage
BTH_I3165 adenosylhomocysteinase from Burkholderia thailandensis E264
52% identity, 99% coverage
D3K115 adenosylhomocysteinase (EC 3.3.1.1) from Leishmania donovani (see paper)
54% identity, 98% coverage
wcw_1005 adenosylhomocysteinase from Waddlia chondrophila WSU 86-1044
52% identity, 96% coverage
- Identification and characterization of the Chlamydia trachomatis L2 S-adenosylmethionine transporter
Binet, mBio 2011 - “...conserved motifs representative of active SahH enzymes were identified in these organisms, i.e., pah_c022o240 and wcw_1005, respectively ( Fig.S3 ). Since out of 26 chlamydial genomes analyzed, only two, P.acanthamoebae and W.chondrophila , appear to have the genes for a complete methylation cycle (SAM synthesis, methylation...”
- “...coccus and W. chondrophila predicted S -adenosylhomocysteine hydrolases (SAHH) amino acid sequences (i.e. pah_c022o240 and wcw_1005, respectively) with those of other organisms. The alignment was generated using the Tcoffee expresso Web server with the SAHH amino acid sequence from Arabidopsis thaliana (gbCAB10173.1), Trypanosoma cruzi (gbAAP45630.1), and...”
3g1uC / Q4Q124 Crystal structure of leishmania major s-adenosylhomocysteine hydrolase
53% identity, 96% coverage
- Ligands: adenosine; nicotinamide-adenine-dinucleotide (3g1uC)
MXAN_6516 adenosylhomocysteinase from Myxococcus xanthus DK 1622
50% identity, 96% coverage
- The lethal cargo of Myxococcus xanthus outer membrane vesicles
Berleman, Frontiers in microbiology 2014 - “...MXAN_5933 Peptidase, M48 (Ste24 endopeptidase) family MXAN_6106 Matrix-associated zinc metalloprotease FibA MXAN_6266 Putative 2,3-cyclic-nucleotide 2-phosphodiesterase MXAN_6516 Adenosylhomocysteinase MXAN_6539 Extracellular protease domain protein MXAN_6601 Peptidase, S9C (acylaminoacyl-peptidase) subfamily MXAN_4796 Fibronectin type III domain protein MXAN_4467 Chaperonin GroEL1 MXAN_4895 Chaperonin GroEL2 MXAN_2538 Adventurous gliding motility protein AgmO MXAN_3060...”
SAHH_BRAEL / A0A087WNH6 Adenosylhomocysteinase; BeSAHase; S-adenosyl-L-homocysteine hydrolase; SAHase; EC 3.13.2.1 from Bradyrhizobium elkanii (see paper)
A0A087WNH6 adenosylhomocysteinase (EC 3.3.1.1) from Bradyrhizobium elkanii (see paper)
52% identity, 97% coverage
- function: May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine, which is a strong inhibitor of SAM-dependent methyltransferases. Catalyzes the hydrolysis of S- adenosyl-L-homocysteine into L-homocysteine and adenosine.
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer; dimer of dimers.
5m5kC / A0A087WNH6 S-adenosyl-l-homocysteine hydrolase from bradyrhizobium elkanii in complex with adenosine and cordycepin (see paper)
52% identity, 98% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine; sodium ion (5m5kC)
NP_001155238 adenosylhomocysteinase isoform 2 from Homo sapiens
56% identity, 93% coverage
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...( Chen et al., 2010 ) and encodes three annotated splicing isoforms (AHCY1 (NP_000678), 2 (NP_001155238), and 3 (NP_001309015), with 432, 404, and 434 amino acids, respectively) that differ only in their first 30 amino acids. These isoforms have not been formally compared side-by-side, although ectopically...”
A4ZHR8 Adenosylhomocysteinase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
49% identity, 94% coverage
pah_c022o240 adenosylhomocysteinase from Parachlamydia acanthamoebae str. Hall's coccus
52% identity, 96% coverage
- Identification and characterization of the Chlamydia trachomatis L2 S-adenosylmethionine transporter
Binet, mBio 2011 - “...possessing the conserved motifs representative of active SahH enzymes were identified in these organisms, i.e., pah_c022o240 and wcw_1005, respectively ( Fig.S3 ). Since out of 26 chlamydial genomes analyzed, only two, P.acanthamoebae and W.chondrophila , appear to have the genes for a complete methylation cycle (SAM...”
- “...strain Halls coccus and W. chondrophila predicted S -adenosylhomocysteine hydrolases (SAHH) amino acid sequences (i.e. pah_c022o240 and wcw_1005, respectively) with those of other organisms. The alignment was generated using the Tcoffee expresso Web server with the SAHH amino acid sequence from Arabidopsis thaliana (gbCAB10173.1), Trypanosoma cruzi...”
ZMO_RS00775 adenosylhomocysteinase from Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
ZMO0182 S-adenosyl-L-homocysteine hydrolase from Zymomonas mobilis subsp. mobilis ZM4
52% identity, 97% coverage
- Improvement of Acetaldehyde Production in Zymomonas mobilis by Engineering of Its Aerobic Metabolism
Kalnenieks, Frontiers in microbiology 2019 - “...dehydrogenase ACTTCGGCATTGGCTTCGTC CGCGTCGCAATATTCAGTTCC ald (Zmob_1390) Aldehyde dehydrogenase CGTATCGCGGGACCGTATCTGA GCGCCCGCTTCTTCCAAAAC cat (ZMO_RS04105) Catalase TCGCCCTTTGAAAACGCATCCTA AACGGGCTTTTTCATCCTCTTTCA gap (ZMO_RS00775) Glyceraldehyde 3 P dehydrogenase ACCCCGGATGTCAGCCTTGTTG GGAGCTGTGCGGATCGGAGTAGAA glk (ZMO_RS01580) Glucokinase GCCGCTTTGGATCGCTTCTGCT ACCGACACCACCGCCAATAACAAC zwf (ZMO_RS01570) Glucose 6 P dehydrogenase AAGTTGCCGGTTACATTGACGA TGCGGATATAGAACGGAACACC cyt (ZMO_RS07025) CydA, subunit I of bd cytochrome quinol oxidase CCTTCCCCGATCCTGTTTTTCTCT...”
- Regulation of biofilm formation in Zymomonas mobilis to enhance stress tolerance by heterologous expression of pfs and luxS
Cao, Frontiers in bioengineering and biotechnology 2023 - “...gene replacement vector with multi clone sites from pUC18 pEX18Tc0182 pEX18Tc containing recombinant sequences flanking ZMO0182 2.2 Construction of AI-2 synthetic pathway Pfs can catalyze SAH to SRH, and LuxS splits SRH to L-homocysteine and S-4,5-dihydroxy-2,3-pentanedione (auto-cyclizing into AI-2) ( Figure 1 ). With the transformation...”
- “...and luxS , Z. mobilis can recover methionine and produce AI-2 simultaneously. SAH hydrolase AhcY (ZMO0182) in Z. mobilis hydrolyzes SAH to L-homocysteine directly without AI-2 production. Knock out of AhcY was conducted to redistribute more carbon flux into the constructed pfs-luxS pathway. 2.3 Construction and...”
HSERO_RS21435 adenosylhomocysteinase from Herbaspirillum seropedicae SmR1
51% identity, 99% coverage
L8H6B5 Adenosylhomocysteinase from Acanthamoeba castellanii (strain ATCC 30010 / Neff)
50% identity, 97% coverage
- Nitroxoline evidence amoebicidal activity against Acanthamoeba castellanii through DNA damage and the stress response pathways
Chen, International journal for parasitology. Drugs and drug resistance 2025 - “...DNA (cytosine-5)-methyltransferas L8GJL0 6.20 16.38 NADH cytochrome b reductase, putative L8H2J2 5.42 15.02 Betainehomocysteine methyltransferase L8H6B5 7.08 10.49 Adenosylhomocysteinas L8H1B7 5.48 2.82 Methionine aminopeptidas L8H7N2 5.74 2.79 Uncharacterized protein L8HK34 5.14 4.25 histone deacetylas Control P07858 (3AI8) 4.30 18.14 Cathepsin B The 18 potential targets were...”
- “...classified as others ( Fig. 5 A Table 1 ). Among them, adenosylhomocysteinase (UniProt ID: L8H6B5 ) and betainehomocysteine methyltransferase (UniProt ID: L8H2J2 ) had relatively better docking scores with nitroxoline (7.08 and 5.42), being stabilised through multiple stacking and polar interactions ( Fig. 5 G...”
XAUB_39520 adenosylhomocysteinase from Xanthomonas citri pv. aurantifolii str. ICPB 11122
XAC0804 adenosylhomocysteinase from Xanthomonas axonopodis pv. citri str. 306
51% identity, 98% coverage
SAHH_MYCTU / P9WGV3 Adenosylhomocysteinase; S-adenosyl-L-homocysteine hydrolase; AdoHcyase; EC 3.13.2.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
P9WGV3 adenosylhomocysteinase (EC 3.3.1.1) from Mycobacterium tuberculosis (see paper)
A1KNQ0 Adenosylhomocysteinase from Mycobacterium bovis (strain BCG / Pasteur 1173P2)
NP_217765 adenosylhomocysteinase from Mycobacterium tuberculosis H37Rv
BCG_3277c putative adenosylhomocysteinase sahH from Mycobacterium bovis BCG str. Pasteur 1173P2
NP_217765, Rv3248c S-adenosyl-L-homocysteine hydrolase from Mycobacterium tuberculosis H37Rv
49% identity, 93% coverage
- function: May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer. - Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...(O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by Clustal Omega (...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...species (UniProt accession code): Lupinus luteus (Q9SP37), Plasmodium falciparum (P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD +...”
- The unfoldase ClpC1 of Mycobacterium tuberculosis regulates the expression of a distinct subset of proteins having intrinsically disordered termini
Lunge, The Journal of biological chemistry 2020 (secret) - S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling
Miller, Journal of bacteriology 2015 - “...P50251, Pyrococcus furiosus; O67240, Aquifex aeolicus; P9WGV3, Mycobacterium tuberculosis (PDB 2ZIZ); Q4Q124, Leishmania major (PDB 3G1U); and P23526,...”
- Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980
Deng, Frontiers in microbiology 2020 - “...Alanine and proline-rich secreted protein Apa 32686.3 6 9.23 16 Mb1891 Rv1860 1 l 3277c A1KNQ0 Adenosylhomocysteinase (ahcY) 54323.3 1 2.82 4 Mb3276c Rv3248c 1 m 3652c A0A0H3MBJ4 Probable conserved membrane protein 27001.5 1 4.94 14 Mb3618c Rv3587c 1 n 3955c A0A0H3MFZ4 Uncharacterized protein 29993.6 1...”
- Mycobacterium tuberculosis S-adenosyl-l-homocysteine hydrolase is negatively regulated by Ser/Thr phosphorylation.
Corrales, Biochemical and biophysical research communications 2013 (PubMed)- GeneRIF: SahH phosphorylation on Thr2 and Thr221 may represent a novel and presumably more general mechanism of regulation of the SAH/SAM balance in mycobacteria.
- Crystal structures of Mycobacterium tuberculosis S-adenosyl-L-homocysteine hydrolase in ternary complex with substrate and inhibitors.
Reddy, Protein science : a publication of the Protein Society 2008 - GeneRIF: crystal structure of SAHH, from in complex with adenosine and nicotinamide adenine dinucleotide; structures of complexes with three inhibitors are also reported
- Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980
Deng, Frontiers in microbiology 2020 - “...were shown to be lipoproteins with potential sites for type II signal peptidase binding. Further, BCG_3277c, BCG_3252, BCG_1154, and BCG_1564 were predicted to be involved in degradation and metabolism of nutrients, BCG_1896 (Apa) was associated with cell adhesion, and BCG_RN06_2923 and BCG_1237c were predicted to play...”
- Evaluation of the cross-immunity between Mycobacterium tuberculosis and Mycobacterium abscessus in vitro
Xu, BMC microbiology 2025 - “...Rv1135c, Rv2098c, Rv2099c, Rv3022c Secretion-related (12) Rv3835, Rv3710, Rv3841, Rv0398c, Rv3106, Rv1371, Rv2874, RV0040C, RV0999, RV3248C, RV0350, RV2241 Latent infection-related (3) Rv2629, Rv2631, Rv0571c M. abscessus and M. tuberculosis shared a total of 1,953 homologous gene clusters (Fig. 3A ). Specifically, M. abscessus had 2030 homologous...”
- Immunogenicity of Mycobacterial Extracellular Vesicles Isolated From Host-Related Conditions Informs About Tuberculosis Disease Status
Schirmer, Frontiers in microbiology 2022 - “...Rv1872c LldD2 1.33 Rv1023 Eno 0.81 Rv3841 BfrB 1.32 Rv3841 BfrB 0.75 Rv1327c GlgE 1.16 Rv3248c SahH 0.74 Rv1133c MetE 1.1 Unknown 1 Rv2818c Hypothetical protein 0.02 0 Regulatory proteins 19 Rv1479 MoxR1 0.16 2 Rv1479 MoxR1 0.26 Rv3295 probably TetR-family 0.11 Rv0144 possibly TetR-family 0.22...”
- Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system
Yu, Nature communications 2021 - “...structure The homology structural model of AgmF was constructed according to the X-ray structure of NP_217765 from Mycobacterium tuberculosis H37Rv (PDB 3CE6, https://www.rcsb.org/structure/3CE6 ) and further refined using Discovery Studio 3.0 (Accelrys). In vitro assay of AgmA Reaction mixtures (50L each) consisted of a solution containing...”
- “...added. c Homology structure model of AgmF. This structure is constructed on the basis of Rv3248c from Mycobacterium tuberculosis H37Rv (PDB 3CE6), and the active 2 and NAD + binding sites are indicated in the rectangular region. The sites marked out are proposed to be essential...”
- emPAI-assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers
Ma, Microbial biotechnology 2021 - “...showed that the areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934 and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value and antibody affinity in mice immunized with recombinant...”
- “...the future. The areas under the curve (AUC) value of Rv2031c, Rv0577, Rv0831c, Rv0934, and Rv3248c were all higher than those of Rv3875 (AUC, 0.6643). Further analysis of the relationship between emPAI value and antibody sensitivity, AUC value, and antibody affinity in mice immunized with recombinant...”
- The In Vivo Transcriptomic Blueprint of Mycobacterium tuberculosis in the Lung
Coppola, Frontiers in immunology 2021 - “...Table S1.6 ), 17 (Rv0005, Rv0284, Rv1161, Rv1297, Rv1398c, Rv1611, Rv1783, Rv1925, Rv2031c, Rv3051c, Rv3219, Rv3248c, Rv3583c, Rv3841, Rv3854c, Rv3874, and Rv3875) were shared with the RT-PCR datasets. Furthermore, a partial validation was performed by using a Mtb RNA-Seq dataset from seven human active TB sputum...”
- “...3716, Data File S1 : Table S1.7 ), eight (Rv3874, Rv0467, Rv1398c, Rv1131, Rv3051c, Rv0284, Rv3248c and Rv1297) were shared with the RT-PCR datasets. We also performed a similar analysis on a published microarray Mtb transcriptome (number of genes=3924) obtained from human sputum samples (n=7) (...”
- Microarray-based selection of a serum biomarker panel that can discriminate between latent and active pulmonary TB
Li, Scientific reports 2021 - “...26.923 Rv3881c* 0.634 97.297 23.077 0.611 96.296 30.769 Rv2236c 0.708 97.297 28.163 0.710 77.778 63.462 Rv3248c 0.680 94.595 25.000 0.491 59.259 53.846 Rv3413c 0.629 97.297 26.923 0.570 88.889 32.692 Rv3803c 0.674 94.595 38.462 0.670 48.148 82.692 a Proteins marked with an asterisk (*) were selected as...”
- Identification of Mycobacterium tuberculosis Peptides in Serum Extracellular Vesicles from Persons with Latent Tuberculosis Infection
Mehaffy, Journal of clinical microbiology 2020 - “...TAV EQAAAELGDTGR Cfp2 (Rv2376c) GSL VEGGIGGTEAR PpiA (Rv0009) IAL FGNHAPK SLA DPNVSFANK VIQ GFMIQGGDPTGTGR SahH (Rv3248c) GVT EETTTGVLR HTI FGEVIDAESQR IHV EALGGHLTK AcpM (Rv2244) IPD EDLAGLR GroES (Rv3418c) DVL AVVSK TVG DVVAYIQK RIP LDVAEGDTVIYSK LEE ENPEAAQALR BfrB (Rv3841) EAL ALALDQER Ag85A (Rv3804c) NDP LLNVGK AGA NLFELENFVAR...”
- One-Year Old Dormant, "Non-culturable" Mycobacterium tuberculosis Preserves Significantly Diverse Protein Profile
Trutneva, Frontiers in cellular and infection microbiology 2020 - “...synthetase GlnA1 (glutamine synthase) (GS-I) Rv2220 122 3 Cytosol Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) Rv3248c N/D 3 Cytosol 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) Rv3418c N/D 4 Membrane Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha chain)...”
- “...protein 70) (heat shock 70 kDa protein) (HSP70) Rv2220 Glutamine synthetase GlnA1 (glutamine synthase) (GS-I) Rv3248c Probable adenosylhomocysteinase SahH (S-adenosyl-L-homocysteine hydrolase) (adohcyase) Rv3418c 10 kDa chaperonin GroES (protein CPN10) (protein GroES) (BCG-a heat shock protein) (10 kDa antigen) Rv3285 Probable bifunctional protein acetyl-/propionyl-coenzyme A carboxylase (alpha...”
- More
Mb3276c PROBABLE ADENOSYLHOMOCYSTEINASE SAHH (S-ADENOSYL-L-HOMOCYSTEINE HYDROLASE) (ADOHCYASE) from Mycobacterium bovis AF2122/97
49% identity, 93% coverage
- Identification of B cell antigenome in Mycobacterium bovis by immunoproteomic analysis
Cho, Acta veterinaria Hungarica 2020 (PubMed)- “...and Mb1868c, which are known B cell antigens, including 13 new proteins, i.e. Mb3751, Mb2006c, Mb3276c, Mb2244, Mb1164c, Mb2553c, Mb2946c, Mb1849c, Mb1511c, Mb1034c, Mb2616c, Mb0854c and Mb2267. These new proteins identified by 2-DE and immunoblotting were the B cell antigens used in developing serological diagnostic methods...”
- Identification of Secreted O-Mannosylated Proteins From BCG and Characterization of Immunodominant Antigens BCG_0470 and BCG_0980
Deng, Frontiers in microbiology 2020 - “...6 9.23 16 Mb1891 Rv1860 1 l 3277c A1KNQ0 Adenosylhomocysteinase (ahcY) 54323.3 1 2.82 4 Mb3276c Rv3248c 1 m 3652c A0A0H3MBJ4 Probable conserved membrane protein 27001.5 1 4.94 14 Mb3618c Rv3587c 1 n 3955c A0A0H3MFZ4 Uncharacterized protein 29993.6 1 2.39 23 Mb3928c/Mb3829c Rv3899c 1 3252 A0A0G2Q9I2...”
6uk3A / L8H6B5 Crystal structure of s-adenosyl-l-homocysteine hydrolase from acanthamoeba castellanii with bound NAD and adenosine
50% identity, 97% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (6uk3A)
BMEI2029 ADENOSYLHOMOCYSTEINASE from Brucella melitensis 16M
52% identity, 95% coverage
PITG_10198 adenosylhomocysteinase from Phytophthora infestans T30-4
49% identity, 96% coverage
- Novel core promoter elements in the oomycete pathogen Phytophthora infestans and their influence on expression detected by genome-wide analysis
Roy, BMC genomics 2013 - “...FPR, and SAASMMS for DPEP. ( B ) Alignment of promoter from P. infestans gene PITG_10198 and its orthologs from P. sojae and P. ramorum . Shown in boxes are the three motifs, which are conserved within the orthologous promoters of the three species, and the...”
- “...and DPEP, a promoter containing all three motifs is shown in Figure 1 B. Gene PITG_10198, which encodes adenosylhomocysteinase, contains a combined INR+FPR block spanning the transcription start site, followed after 5 bases by a DPEP. All three motifs are well-conserved in the orthologous promoters from...”
3ce6A / P9WGV3 Crystal structure of mycobacterium tuberculosis s-adenosyl-l- homocysteine hydrolase in ternary complex with NAD and adenosine (see paper)
49% identity, 94% coverage
- Ligands: adenosine; nicotinamide-adenine-dinucleotide (3ce6A)
BAB1_2099 S-adenosyl-L-homocysteine hydrolase:ATP/GTP-binding site motif A (P-loop) from Brucella melitensis biovar Abortus 2308
52% identity, 98% coverage
- Intracellular adaptation of Brucella abortus
Lamontagne, Journal of proteome research 2009 - “...Transporters BCSP31 46 BAB2_1125 AA synthesis N-Trans III-like 46 BAB1_1351 Transporters Trs-ABC sulfate binding 47 BAB1_2099 AA synthesis AhcY 47 BAB1_0214 Transporters Trs-ABC sulfonate/nitrate 48 BAB1_1399 AA synthesis Ilvc 48 BAB2_0023 Transporters Trs-ABC branched amino acid 49 BAB1_2150 Stress response Dps 49 BAB2_0493 Transporters UgpC 50...”
Q8FRJ4 adenosylhomocysteinase (EC 3.3.1.1) from Corynebacterium efficiens (see paper)
49% identity, 97% coverage
MAV_4211 adenosylhomocysteinase from Mycobacterium avium 104
49% identity, 92% coverage
MAP3362c SahH from Mycobacterium avium subsp. paratuberculosis str. k10
49% identity, 92% coverage
SMc02755 PROBABLE ADENOSYLHOMOCYSTEINASE PROTEIN from Sinorhizobium meliloti 1021
51% identity, 98% coverage
- A stress-induced small RNA modulates alpha-rhizobial cell cycle progression
Robledo, PLoS genetics 2015 - “..., mltB2 , and the genes coding for the PilZ-like protein SMc00999, the adenosylhomocystein hydrolase SMc02755, the putative transcriptional regulator SMc01842 and the hypothetical protein SMc03149. Beside this, in both media most of the differentially expressed genes with known functions were also related to metabolism. Among...”
- Transcriptome analysis of the role of GlnD/GlnBK in nitrogen stress adaptation by Sinorhizobium meliloti Rm1021
Yurgel, PloS one 2013 - “...), and probable methylenetetrahydrofolate reductase oxidoreductase ( metF ). Additionally, this cluster included probable adenosylhomocysteinase (SMc02755), putative phosphoglycerate dehydrogenase ( serA ), and succinoglycan regulator exsB [30] , [31] . The expression pattern of Cluster I.III suggested that (i) there is another regulatory system that represses...”
- An orphan LuxR homolog of Sinorhizobium meliloti affects stress adaptation and competition for nodulation
Patankar, Applied and environmental microbiology 2009 - “...GCCCTTTCCGATCT CCTC SMc01109 (metK) GGGCATCATGTTCG GCTATG SMc02755 (ahcY) CGGACCCTGCTGCT TGAC SMc01843 (metF) GATTATTGATCGGCA GTATCC Reverse primer RESULTS VOL....”
- “...and adenosine by S-adenosyl-L-homocysteine hydrolase, encoded by ahcY (SMc02755) (Fig. 2) (26, 49). Recycled homocysteine can then accept a methyl group from...”
H16_A0244 Adenosylhomocysteinase from Ralstonia eutropha H16
H16_A0244 adenosylhomocysteinase from Cupriavidus necator H16
51% identity, 97% coverage
- Investigations on the microbial catabolism of the organic sulfur compounds TDP and DTDP in Ralstonia eutropha H16 employing DNA microarrays
Peplinski, Applied microbiology and biotechnology 2010 - “...Enoyl-CoA hydratase/carnithine racemase 3.25 4.35 5.07 2.98 H16_A0217 Hypothetical membrane-associated protein 5.77 13.37 46.08 21.53 H16_A0244 ahcY Adenosylhomocysteinase 5.55 10.25 10.90 13.39 H16_A1070 Acyl dehydratase 4.44 4.70 2.79 2.59 H16_B0782 Acetyltransferase, GNAT-family 38.41 428.83 27.49 25.35 H16_B1784 Signal transduction histidine kinase 4.17 8.26 3.62 2.67 H16_B1785...”
- “...during cultivation in presence of DTDP (6- to 46-fold). ahcY , encoding the S -adenosylhomocysteinase (H16_A0244, 6- to 13-fold) which catalyzes the reversible hydrolysis of S -adenosylhomocysteine to adenosine and l -homocysteine, exhibited increased expression levels. It has to be mentioned that this gene was detected...”
3n58B / Q2YQX8 Crystal structure of s-adenosyl-l-homocysteine hydrolase from brucella melitensis in ternary complex with NAD and adenosine, orthorhombic form
52% identity, 97% coverage
- Ligand: nicotinamide-adenine-dinucleotide (3n58B)
EHI_068250 adenosylhomocysteinase, putative from Entamoeba histolytica HM-1:IMSS
50% identity, 98% coverage
- Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica
Husain, BMC genomics 2011 - “...CS3, EHI_060340; MAT, 70.m00173; MGL1, EHI_144610; MGL2, EHI_142250; NifS; EHI_136380; PGDH, EHI_060860; PSAT, EHI_026360; SAHH, EHI_068250; SAT2, EHI_021570; SAT3, EHI_153430. We have recently shown by metabolomic analysis that the synthesis of OAS and SMC markedly increased upon L-cysteine deprivation in E. histolytica . OAS in bacteria...”
BT_2797 adenosylhomocysteinase from Bacteroides thetaiotaomicron VPI-5482
50% identity, 97% coverage
- Gut Commensal Bacteroidetes Encode a Novel Class of Vitamin B12-Binding Proteins
Putnam, mBio 2022 - “...Requires adenosylcobalamin as a cofactor 26 , 27 BT_2419 Putative GMP synthase [glutamine-hydrolyzing] 0.91873212 0.97976994 BT_2797 Adenosylhomocysteinase 1.05377016 1.33389028 BT_3841 FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor) 0.79644085 4.87735737 BT_4307 Putative glycogen synthase 2.61230509 1.05085244 BT_4612 Hypothetical protein 2.29627437 1.40787681 a B 12 -ABP versus unlabeled B...”
flI / CAJ20010.1 adenosyl-homocysteine hydrolase from Streptomyces cattleya (see paper)
47% identity, 94% coverage
CCNA_00257 adenosylhomocysteinase from Caulobacter crescentus NA1000
CC0257, CC_0257 adenosylhomocysteinase from Caulobacter crescentus CB15
53% identity, 98% coverage
- Growth-driven displacement of protein aggregates along the cell length ensures partitioning to both daughter cells in Caulobacter crescentus
Schramm, Molecular microbiology 2019 - “...category was determined to obtain relative abundance. C. Localization pattern of the aggregationprone proteins GyrB, CCNA_00257 and EFG at 30C and 40C. Expression of the fusion proteins was induced for 3h at 30C, followed by 1h exposure to 40C or 0.25M kanamycin. Scale bar is 5m....”
- “...the proteins GyrB, EFG (elongation factor G, encoded by fusA ), and the predicted homocysteinase CCNA_00257 to monitor their subcellular localization when exposed to heat and kanamycin stress (Fig. 2 C). All three proteins were confirmed to condense into aggregate foci colocalizing with DnaK when exposed...”
- Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...3.12 CC_0050 CCNA_00048 S-adenosylmethionine synthetase 3.05 CC_0167 CCNA_00166 Hypothetical protein (transglutaminase-like cysteine proteinase) 2.22 CC_0257 CCNA_00257 Adenosylhomocysteinase 3.12 CC_0482 CCNA_00515 Cobalamin-independent methionine synthase (Zinc) 2.49 CC_0984 CCNA_01035 Gamma-glutamyltranspeptidase 2.68 CC_1048 CCNA_01100 Acylamino-acid-releasing enzyme 2.73 CC_2137 CCNA_02221 Methionine synthase I metH (Zinc) 2.52 CC_2138 CCNA_02222 5-methyltetrahydrofolate 2.72...”
- Global transcriptional response of Caulobacter crescentus to iron availability
da, BMC genomics 2013 - “...in methionine biosynthesis, such as methionine synthases (CC0482, CC2137, CC2138), adenosylmethionine synthtase (CC0050), S-adenosyl-L-homocysteine hydrolase (CC0257) and methylenetetrahydrofolate reductase (CC2140), which is required to produce 5-methyltetrahydrofolate as methyl-group donor for methionine synthesis. Pathways of protein catabolism were also repressed by iron limitation as revealed by downregulation...”
- “...methyltransferase 3.12 CC_0050 CCNA_00048 S-adenosylmethionine synthetase 3.05 CC_0167 CCNA_00166 Hypothetical protein (transglutaminase-like cysteine proteinase) 2.22 CC_0257 CCNA_00257 Adenosylhomocysteinase 3.12 CC_0482 CCNA_00515 Cobalamin-independent methionine synthase (Zinc) 2.49 CC_0984 CCNA_01035 Gamma-glutamyltranspeptidase 2.68 CC_1048 CCNA_01100 Acylamino-acid-releasing enzyme 2.73 CC_2137 CCNA_02221 Methionine synthase I metH (Zinc) 2.52 CC_2138 CCNA_02222 5-methyltetrahydrofolate...”
R7QHD1 Adenosylhomocysteinase from Chondrus crispus
47% identity, 93% coverage
NCgl0719 adenosylhomocysteinase from Corynebacterium glutamicum ATCC 13032
49% identity, 97% coverage
- Role of Corynebacterium glutamicum sprA encoding a serine protease in glxR-mediated global gene regulation
Hong, PloS one 2014 - “...diverse cellular functions such as energy and carbon metabolism (NCgl2809), nitrogen metabolism (NCgl0049), methylation reactions (NCgl0719), and peptidoglycan biosynthesis (NCgl1267), as well as stress, starvation, and survival (NCgl0938) were affected and showed decreased transcription. Taken together, these data suggest that SprA, as a serine protease, performs...”
- “...following primers were used: glxR , 5-CACCGAAGTTCATGCAGCAACCAT-3 and 5-TTAGCCAGCTGCAGAAGGGTCTTA-3 ; NCgl0550, 5-AGCTTGGCCGCTCTGTTTATGTTG-3 and 5-AGTTGCTAGTTGGTGAGCTTGGGA-3 ; NCgl0719, 5-AGAGCCAGCTGGTGTTCCAGTATT-3 and 5-TCGAGGATCATGTTTGGCAGCTCA-3 ; NCgl0938, 5-AGCCATCGTCCCAATTTCAAAGGC-3 and 5-TGATCTCATCGGTGACAACTCGCA-3 ; NCgl2809, 5-TCACCTGCGAAAGCTCCGATAACA-3 and 5-ATTACGCGCATCTTCGCACCAAAG-3 ; and 16S ribosomal RNA, 5-CGGCCTATCAGCTTGTTGGT-3 and 5-TGGGCCGTGTCTCAGTCC-3 . Plasmid and Strain Construction Plasmid pSL500 harboring glxR...”
SAMCCGM7_Ch0041 adenosylhomocysteinase from Sinorhizobium americanum CCGM7
51% identity, 98% coverage
RL0031 putative S-adenosyl-L-homocysteine hydrolase from Rhizobium leguminosarum bv. viciae 3841
50% identity, 98% coverage
- Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere
Garcia-Fraile, Plant and soil 2015 - “...carrying nif HSpc Tett et al. ( 2014 ) X365 (H1K1F06-1B08) 3841 carrying mTn5 in RL0031 This study X376 (H2K2B08-1C04) 3841 carrying mTn5 in RL0634 This study X377(H2K2C06-1B06) 3841 carrying mTn5 in pRL90234 This study X378H5K5F04-1A10) 3841 carrying mTn5 in RL1109 This study X379 (H2K2B02-1B05) 3841...”
- “...This study X385(H2K2C07-1E03) 3841 carrying mTn5 in RL0079 This study RU4372 300 carrying mTn5 in RL0031 This study A1398 300 carrying mTn5 in RL0634 This study A1399 300 carrying mTn5 in pRL90234 This study A1401 300 carrying mTn5 in RL0811A This study A1402 300 carrying mTn5...”
V5I095 Adenosylhomocysteinase (Fragment) from Ixodes ricinus
49% identity, 98% coverage
SAHH3_BOVIN / A6QLP2 Adenosylhomocysteinase 3; AdoHcyase 3; IP(3)Rs binding protein released with IP(3) 2; IRBIT2; Long-IRBIT; EC 3.13.2.1 from Bos taurus (Bovine) (see paper)
48% identity, 75% coverage
- function: May regulate the electrogenic sodium/bicarbonate cotransporter SLC4A4 activity and Mg(2+)-sensitivity. On the contrary of its homolog AHCYL1, does not regulate ITPR1 sensitivity to inositol 1,4,5-trisphosphate (By similarity).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) Note=Binds 1 NAD(+) per subunit
subunit: Homotetramer. Forms heteromultimers with AHCYL1 (via the C- terminal region). Interacts with ITPR1; with lower affinity than AHCYL1 and maybe via ITPR1 (By similarity). Interacts with SLC4A4 (PubMed:24472682). Interacts with ZCCHC4 (By similarity).
XP_005205764 adenosylhomocysteinase 3 isoform X1 from Bos taurus
48% identity, 76% coverage
SAHH3_HUMAN / Q96HN2 Adenosylhomocysteinase 3; AdoHcyase 3; IP(3)Rs binding protein released with IP(3) 2; IRBIT2; Long-IRBIT; S-adenosyl-L-homocysteine hydrolase 3; S-adenosylhomocysteine hydrolase-like protein 2; EC 3.13.2.1 from Homo sapiens (Human) (see 2 papers)
NP_056143 adenosylhomocysteinase 3 isoform a from Homo sapiens
48% identity, 75% coverage
- function: May regulate the electrogenic sodium/bicarbonate cotransporter SLC4A4 activity and Mg(2+)-sensitivity. On the contrary of its homolog AHCYL1, does not regulate ITPR1 sensitivity to inositol 1,4,5-trisphosphate (PubMed:19220705).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) Note=Binds 1 NAD(+) per subunit
subunit: Homotetramer (Probable). Forms heteromultimers with AHCYL1 (via the C-terminal region) (PubMed:19220705). Interacts with ITPR1; with lower affinity than AHCYL1 and maybe via ITPR1 (PubMed:19220705). Interacts with SLC4A4 (By similarity). Interacts with ZCCHC4 (PubMed:31799605). - Genetic Polymorphism of Zinc Transporter-8 Gene (SLC30A8), Serum Zinc Concentrations, and Proteome Profiles Related to Type 2 Diabetes in Elderly.
Sirivarasai, Journal of clinical medicine 2025 - “...factor (FGF) 2.612 F8WDK8 Ribosomal protein L22 like 1 2.477 9 C9JHJ5 Golgin A4 2.610 Q96HN2 Adenosylhomocysteinase 3 (AdoHcyase 3) 2.477 10 A0A087WVA7 IQ motif containing with AAA domain 1 like 2.606 Q5T0Y8 Sphingomyelin phosphodiesterase acid like 3B 2.478 11 E9PNZ4 Microtubule actin crosslinking factor 1...”
- Longitudinal Assessment of Nasopharyngeal Biomarkers Post-COVID-19: Unveiling Persistent Markers and Severity Correlations.
Redondo-Calvo, Journal of proteome research 2024 - “...KIF5B P33176 GAK cyclin-G-associated kinase 1.54 1.73 GAK O14976 SAHH3# adenosylhomocysteinase 3 1.79 1.75 AHCYL2 Q96HN2 IPO5 importin-5 3.98 1.76 IPO5 O00410 MCTS1# malignant T-cell-amplified sequence 1 1.74 1.76 MCTS1 Q9ULC4 NADAP# kanadaptin 1.50 1.76 SLC4A1AP Q9BWU0 PYRG2 CTP synthase 2 2.19 1.76 CTPS2 Q9NRF8 MCES...”
- Human amygdala involvement in Alzheimer's disease revealed by stereological and dia-PASEF analysis.
Gonzalez-Rodriguez, Brain pathology (Zurich, Switzerland) 2023 - “...reductase [NADPH] 3 2.22 0.0045 O00299 CLIC1_HUMAN CLIC1 Chloride intracellular channel protein 1 2.19 0.0008 Q96HN2 SAHH3_HUMAN AHCYL2 Adenosylhomocysteinase 3 2.18 0.0217 P53367 ARFP1_HUMAN ARFIP1 Arfaptin1 2.16 0.0484 P45880 VDAC2_HUMAN VDAC2 Voltagedependent anionselective channel protein 2 2.16 0.0205 P10644 KAP0_HUMAN PRKAR1A cAMPdependent protein kinase type Ialpha...”
- Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics.
Sun, BMC pregnancy and childbirth 2018 - “...carrier family 13 Q59HF0 N/A 0.781 0.009 MRG/MORF4L-binding protein Q9NV56 MRGBP 0.776 0.042 Adenosylhomocysteinase 2 Q96HN2 AHCYL2 0.746 0.028 Polyadenylate-binding protein 4 B1ANR0 PABPC4 0.744 0.025 Fig. 1 K-means clustering of differentially expressed proteins identified in human syncytiotrophoblast Immunoblotting verification To verify the expression levels of...”
- Preliminary analysis of the protein profile in saliva during physiological term and preterm delivery.
Łopucki, Molecular medicine reports 2018 - “...8.23 23 Leukemia inhibitory factor receptor P42702 (LIFR_HUMAN) 133 16 123.74 5.50 25 Adenosylhomocysteinase 3 Q96HN2 (SAHH3_HUMAN) 160 19 66.72 7.13 29 Guanylyl cyclase-activating protein 1 P43080 (GUC1A_HUMAN) 62 57 22.92 4.34 pI, isoelectric point. Mascot Scores greater than 67 were considered significant at (P<0.05)....”
- Mechanisms Underlying Adaptation to Life in Hydrogen Sulfide-Rich Environments.
Kelley, Molecular biology and evolution 2016 - “...TCONS_00011212 Q5FVE4 Long-chain-fatty-acid-CoA ligase ACSBG2 acsbg1 1 TCONS_00011532 Q9HBU6 Ethanolamine kinase 1 etnk1 1 TCONS_00011703 Q96HN2 Adenosylhomocysteinase 3 ahcyl1 (3 of 3) 1 TCONS_00011898 Q9UPQ4 Tripartite motif-containing protein 35 trim35-12 1 TCONS_00012317 O60825 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 pfkfb2a 1 TCONS_00013668 Q9NZW5 MAGUK p55 subfamily member 6 mpp6b 1...”
- New p53 related genes in human tumors: significant downregulation in colon and lung carcinomas.
LLeonart, Oncology reports 2006 (PubMed)- GeneRIF: Downregulation of KIAA0828 is associated with colon and lung carcinomas
SAHH3_MOUSE / Q68FL4 Putative adenosylhomocysteinase 3; AdoHcyase 3; Long-IRBIT; S-adenosyl-L-homocysteine hydrolase 3; S-adenosylhomocysteine hydrolase-like protein 2; EC 3.13.2.1 from Mus musculus (Mouse) (see paper)
48% identity, 75% coverage
- function: May regulate the electrogenic sodium/bicarbonate cotransporter SLC4A4 activity and Mg(2+)-sensitivity. On the contrary of its homolog AHCYL1, does not regulate ITPR1 sensitivity to inositol 1,4,5-trisphosphate.
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer. Forms heteromultimers with AHCYL1 (via the C- terminal region). Interacts with ITPR1; with lower affinity than AHCYL1 and maybe via ITPR1. Interacts with SLC4A4. Interacts with ZCCHC4 (By similarity). - Mass spectrometry-based draft of the mouse proteome
Giansanti, Nature methods 2022 - “...the gene Ahcyl2. In the left panel, the amino acid sequence of the canonical protein (Q68FL4) is shown, along with the three alternative products. Portions of the sequences identified in our dataset and which discriminate between the 4 isoforms are highlighted. In the right panel, a...”
- Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury
Noble, Frontiers in molecular neuroscience 2018 - “...5 Protein Wnt-3a P27467 39 0 2 Prothrombin P19221 70 2 0 Putative adenosylhomocysteinase 3 Q68FL4 67 1 2 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial P35486 43 2 0 Pyruvate kinase isozymes M1/M2 P52480 58 16 2 Ras GTPase-activating-like protein IQGAP1 Q9JKF1 189...”
- Differential proteomic and behavioral effects of long-term voluntary exercise in wild-type and APP-overexpressing transgenics.
Rao, Neurobiology of disease 2015 - “...Q3THG0 ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit Atp5o 23.4 4 - <.01 Q68FL4 Adenosylhomocysteinase 3 Ahcyl2 66.8 4 0.2 <.05 O70250 Phosphoglycerate mutase 2 Pgam2 28.8 2 3.4 <.01 Glycolysis/gluconeogenesis P40142 Transketolase Tkt 67.6 14 0.7 <.05 P45376 Aldose reductase Akr1b1 35.7 3...”
G1T1W6 Adenosylhomocysteinase like 2 from Oryctolagus cuniculus
48% identity, 70% coverage
NP_958450 S-adenosylhomocysteine hydrolase-like protein 1 isoform 1 from Danio rerio
48% identity, 83% coverage
F8WI65 Adenosylhomocysteinase 3 isoform 4 from Mus musculus
48% identity, 91% coverage
Swit_2674 S-adenosyl-L-homocysteine hydrolase from Sphingomonas wittichii RW1
51% identity, 98% coverage
- Proteomic profiling of the dioxin-degrading bacterium Sphingomonas wittichii RW1
Colquhoun, Journal of biomedicine & biotechnology 2012 - “...1,2-dioxygenase 1.8 0.078 921 148555809 Swit_2901 Putative cold shock DNA-binding domain 1.89 0.078 539 148555586 Swit_2674 Adenosylhomocysteinase 1.97 0.062 418 148553385 Swit_0461 Elongation factor Ts 2.10 0.05 934 115279619 Swit_3055 Meta -cleavage pathway hydrolase 2.54 0.055 603 148555952 Swit_3046 Glyoxalase/bleomycin resistance protein/ dioxygenase 3.88 0.031 a...”
MMSR116_19640, MMSR116_RS19390 adenosylhomocysteinase from Methylobacterium mesophilicum SR1.6/6
51% identity, 98% coverage
- Transcriptome and Secretome Analyses of Endophyte Methylobacterium mesophilicum and Pathogen Xylella fastidiosa Interacting Show Nutrient Competition
Dourado, Microorganisms 2023 - “...20 8 44 - MMSR116_RS13495 MMSR116_13665 Pyruvate, phosphate dikinase 97 2 - 16 MMSR116_RS19390 *** MMSR116_19640 Adenosylhomocysteinase AhcY 51 5 - 17 MMSR116_RS19735 *** MMSR116_19990 Phosphate-binding protein PstS 37 6 23|24 - 22 **** MMSR116_18145 *** Porin 59 3 0.89 * Number of the locus tag...”
- “...Other down-regulated genes include those of amino acid biosynthesis functions: the L-homocysteine biosynthesis gene Adenosylhomocysteinase (MMSR116_RS19390); serine biosynthesis genes, such as D-serine ammonia-lyase (MMSR116_RS11755), serine protease (MMSR116_RS01620), serine O-acetyltransferase (MMSR116_RS25615), and PrkA family serine protein kinase (MMSR116_RS24210); besides other amino acids related genes: a hypothetical protein...”
- “...proteins in the co-culture for Mm : the inorganic pyrophosphatase Ppa (MMSR116_RS21075), the adenosylhomocysteinase AhcY (MMSR116_RS19390), the phosphate-binding protein PstS (MMSR116_RS19735) and a porin (MMSR116_18145) ( Table 3 and Figure 5 ). On the other hand, in Mm monoculture, there were 25 exclusive proteins. MMSR116_RS18845, flagellin,...”
Dshi_3426 adenosylhomocysteinase from Dinoroseobacter shibae DFL 12
49% identity, 98% coverage
- Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site dif
Wang, mSystems 2021 - “...biogenesis/integrity 4.41E03 9.78E04 0.44 Dshi_0821 Glycine hydroxymethyltransferase Intracellular protein Amino acid metabolism 4.26E03 8.83E04 0.43 Dshi_3426 Adenosylhomocysteinase Intracellular protein Amino acid metabolism 4.25E03 3.04E04 0.43 Dshi_2156 Fructose-bisphosphate aldolase Intracellular protein Carbon and energy metabolism 4.18E03 6.10E04 0.42 Dshi_0825 AMP-dependent synthetase and ligase Intracellular protein Not assigned...”
Dde_3134 Adenosylhomocysteinase from Desulfovibrio desulfuricans G20
49% identity, 95% coverage
Q01781 Adenosylhomocysteinase from Petroselinum crispum
47% identity, 97% coverage
B7FT14 Adenosylhomocysteinase from Phaeodactylum tricornutum (strain CCAP 1055/1)
47% identity, 95% coverage
SAHH2_DROME / Q9VZX9 Adenosylhomocysteinase-like 1; dAhcyL1; Inactive S-adenosyl-L-homocysteine hydrolase; S-adenosylhomocysteine hydrolase-like protein 1 from Drosophila melanogaster (Fruit fly) (see paper)
47% identity, 88% coverage
- function: Might play a role in the regulation of methionine metabolism possibly by binding and inactivating Ahcy.
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Interacts with Ahcy; the interaction may negatively regulate Ahcy catalytic activity.
disruption phenotype: RNAi-mediated knockdown results in extended lifespan with increased fecundity and suppression of age-related decreased climbing activity and intestinal integrity (PubMed:27313316). Does not affect oxidative stress resistance (PubMed:27313316). Decreases levels of S-adenosyl-homocysteine and homocysteine (PubMed:27313316). Suppresses histone H3K4 trimethylation (PubMed:27313316). RNAi-mediated knockdown in neurons results in extended life span (PubMed:27313316). RNAi-mediated knockdown in the fat body does not show any phenotype (PubMed:27313316). Simultaneous knockdown of AhcyL2 in intestine results in extended life span (PubMed:27313316). - A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites
Mishra, Life science alliance 2024 - “...CG4202 Q9I7W5 103352/KK ++ 49946/GD + Something about silencing 10 UTP3 Q9NQZ2 37.2 611614 CG9977 Q9VZX9 106749/KK ++ 36193/GD ++ Adenosylhomocysteinase-like 1 AHCYL1 O43865 72.87 607826 CG32847 Q8IQM1 104294/KK ++ 48423/GD ++ UN, contains the RING (Really Interesting New Gene) finger domain TRIM26 Q12899 21.25 600830...”
- A genetic screen to uncover molecular mechanisms underlying lipid transfer protein function at membrane contact sites and neurodegeneration
Mishra, 2023
3gvpA / Q96HN2 Human sahh-like domain of human adenosylhomocysteinase 3
47% identity, 97% coverage
- Ligand: nicotinamide-adenine-dinucleotide (3gvpA)
F6GTM7 Adenosylhomocysteinase from Vitis vinifera
48% identity, 94% coverage
I3QHX5 Adenosylhomocysteinase from Nicotiana benthamiana
48% identity, 94% coverage
XP_006492541 adenosylhomocysteinase from Citrus sinensis
47% identity, 96% coverage
LOC107783029 adenosylhomocysteinase-like from Nicotiana tabacum
48% identity, 94% coverage
- Identification and Characterization of Plant-Interacting Targets of Tomato Spotted Wilt Virus Silencing Suppressor
Zhai, Pathogens (Basel, Switzerland) 2021 - “...Arabidopsis Carbonic anhydrase LOC107768773 Solyc02g086820 AT3G01500 Heat shock protein 90 (HSP90) LOC107768797 Solyc12g015880 AT5G56000 Adenosylhomocysteinase LOC107783029 Solyc09g092380 AT4G13940 Chloroplast photosystem II subunit O2 (PSBO2) LOC107766588 Solyc02g065400 AT3G50820 Chloroplast photosystem II subunit P1 (PSBP1) LOC107830202 Solyc07g044860 AT1G06680 MP-interacting protein (MIP) 1.2 LOC107801992 Solyc04g009770 AT3G44110 Heat shock protein...”
P68173 Adenosylhomocysteinase from Nicotiana tabacum
P68172 Adenosylhomocysteinase from Nicotiana sylvestris
48% identity, 94% coverage
K7QK65 Adenosylhomocysteinase from Capsicum annuum
47% identity, 94% coverage
- Identification of the cell wall proteins associated with the softening of Lycium barbarum L. fruit by using iTRAQ technology.
Liu, Food chemistry. Molecular sciences 2022 - “...thiolase 2, peroxisomal-like 1.0730.034 1.4480.031 0.000 1.349 F1DBB9 Chloroplast polyphenol oxidase 1.1690.078 0.7410.021 0.034 0.634 K7QK65 Adenosylhomocysteinase 1.0010.022 1.2560.026 0.000 1.255 M1AIT2 D-3-phosphoglycerate dehydrogenase 1.0290.029 0.8010.022 0.001 0.779 M1B9T8 Aspartate aminotransferase 1.0300.049 1.2370.079 0.034 1.201 M1C0V6 Fructose-bisphosphate aldolase 0.8890.034 0.7030.057 0.017 0.791 P50433 Serine hydroxymethyltransferase, mitochondrial...”
Q9SP37 adenosylhomocysteinase (EC 3.3.1.1) from Lupinus luteus (see paper)
47% identity, 94% coverage
- Combining crystallographic and binding affinity data towards a novel dataset of small molecule overlays.
Hönig, Journal of computer-aided molecular design 2024 - “...IDs with multiple UniProt accession numbers can be due to differing organisms (e.g., Q3JY79 and Q9SP37 for cluster RAB_A_60-3glq), virus strains (e.g., C6KP13 and C3W5S3 for cluster LNV_B_801-3ti3 or proteins with identical binding sites (e.g., P45984, P53779 and P45983 for cluster 880_A_502-4z91). The reasons for all...”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...(P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by Clustal Omega ( https://www.ebi.ac.uk/Tools/msa/clustalo/ ) and...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...LlSAHase. The alignment includes the sequences of the following species (UniProt accession code): Lupinus luteus (Q9SP37), Plasmodium falciparum (P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima...”
- Evolutionary conservation and post-translational control of S-adenosyl-L-homocysteine hydrolase in land plants
Alegre, PloS one 2020 - “...sequences from Arabidopsis thaliana (accession AT4G13940; The Arabidopsis Information Resource, www.arabidopsis.org ), Lupinus luteus (accession Q9SP37; https://www.uniprot.org ), Brassica oleracea convar. capitata , (accession Bol033424; https://phytozome.jgi.doe.gov/pz/portal.html ), Spinacia oleracea (accession A0A0K9RFV6; https://www.uniprot.org ) and Physcomitrella patens (accession Pp3c19_13810V3.1; https://phytozome-next.jgi.doe.gov/ ) were aligned using ClustalW Multiple Alignment...”
- Distinct colonization patterns and cDNA-AFLP transcriptome profiles in compatible and incompatible interactions between melon and different races of Fusarium oxysporum f. sp. melonis.
Sestili, BMC genomics 2011 - “...-1 -1 -1 0 0 0 0 0 0 0 -1 P996b CT-TG 151 -- Q9SP37 Adenosylhomocysteise 8E-15 Metabolism 0 -1 -1 -1 0 0 0 -1 0 0 0 -1 P1336 TC-GA 332 -- Q39110 Gibberellin 20 oxidase 1 2E-45 Metabolism 1 0 0 1...”
XP_002950429 uncharacterized protein from Volvox carteri f. nagariensis
46% identity, 95% coverage
3ondB / Q9SP37 Crystal structure of lupinus luteus s-adenosyl-l-homocysteine hydrolase in complex with adenosine (see paper)
47% identity, 94% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine; sodium ion (3ondB)
SAHH2_HUMAN / O43865 S-adenosylhomocysteine hydrolase-like protein 1; DC-expressed AHCY-like molecule; IP(3)Rs binding protein released with IP(3); IRBIT; Putative adenosylhomocysteinase 2; S-adenosyl-L-homocysteine hydrolase 2; AdoHcyase 2 from Homo sapiens (Human) (see 11 papers)
SAHH2_MOUSE / Q80SW1 S-adenosylhomocysteine hydrolase-like protein 1; IP3R-binding protein released with inositol 1,4,5-trisphosphate; Putative adenosylhomocysteinase 2; S-adenosyl-L-homocysteine hydrolase 2; AdoHcyase 2 from Mus musculus (Mouse) (see 8 papers)
G1SXT1 Adenosylhomocysteinase like 1 from Oryctolagus cuniculus
NP_663517 S-adenosylhomocysteine hydrolase-like protein 1 isoform 1 from Mus musculus
XP_008759627 S-adenosylhomocysteine hydrolase-like protein 1 isoform X2 from Rattus norvegicus
48% identity, 87% coverage
- function: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate, competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. Promotes the formation of contact points between the endoplasmic reticulum (ER) and mitochondria, facilitating transfer of Ca(2+) from the ER to mitochondria (PubMed:27995898). Under normal cellular conditions, functions cooperatively with BCL2L10 to limit ITPR1- mediated Ca(2+) release but, under apoptotic stress conditions, dephosphorylated which promotes dissociation of both AHCYL1 and BCL2L10 from mitochondria-associated endoplasmic reticulum membranes, inhibits BCL2L10 interaction with ITPR1 and leads to increased Ca(2+) transfer to mitochondria which promotes apoptosis (PubMed:27995898). In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (PubMed:16793548). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates from ITPR1 to interact with CFTR and SLC26A6, mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (PubMed:16769890). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed:20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition (PubMed:18829453). May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state (PubMed:19224921). Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (PubMed:25237103). In vitro does not exhibit any S-adenosyl-L- homocysteine hydrolase activity (By similarity).
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Forms multimers (PubMed:16793548). Forms heteromultimers with AHCYL2 (via the C-terminal region) (PubMed:19220705). Interacts (when phosphorylated) with ITPR1 (when not phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1 (PubMed:16793548, PubMed:27995898). Interacts with BCL2L10; this strengthens the interaction of AHCYL1 with ITPR1 (PubMed:27995898). Interacts with CFTR and SLC26A6; the interactions take place once AHCYL1 is released from ITPR1 and increase CFTR and SLC26A6 activities (By similarity). Interacts with RRM1; in a phosphorylation- and (dATP)- dependent manner. Interacts (via PEST domain when phosphorylated) with SLC4A4 isoform 1 but not isoform 2; the interaction increases SLC4A4 isoform 1 activity (PubMed:16769890). Interacts (when phosphorylated) with SLC9A3; the interaction is required for SLC9A3 apical location and activity (PubMed:18829453, PubMed:20584908). Interacts (when phosphorylated) with FIP1L1; the interaction is direct and associates AHCYL1 with the CPSF complex and RNA (PubMed:19224921). Interacts with PAPOLA (PubMed:19224921). Interacts with ZCCHC4 (PubMed:31799605). Interacts with AHCY (PubMed:28647132). - function: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate, competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. Promotes the formation of contact points between the endoplasmic reticulum (ER) and mitochondria, facilitating transfer of Ca(2+) from the ER to mitochondria (By similarity). Under normal cellular conditions, functions cooperatively with BCL2L10 to limit ITPR1- mediated Ca(2+) release but, under apoptotic stress conditions, dephosphorylated which promotes dissociation of both AHCYL1 and BCL2L10 from mitochondria-associated endoplasmic reticulum membranes, inhibits BCL2L10 interaction with ITPR1 and leads to increased Ca(2+) transfer to mitochondria which promotes apoptosis (By similarity). In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (By similarity). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates from ITPR1 to interact with CFTR and SLC26A6, mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (PubMed:12525476, PubMed:23542070). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (PubMed:19224921). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (PubMed:19033647, PubMed:21317537). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2. Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2- dependent calcium inhibition. May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state. Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (By similarity). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (PubMed:12525476).
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Forms multimers (By similarity). Forms heteromultimers with AHCYL2 (via the C-terminal region) (PubMed:19220705). Interacts (when phosphorylated) with ITPR1 (when not phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1 (PubMed:23542070). Interacts with BCL2L10; this strengthens the interaction of AHCYL1 with ITPR1 (By similarity). Interacts with CFTR and SLC26A6; the interactions take place once AHCYL1 is released from ITPR1 and increase CFTR and SLC26A6 activities (PubMed:19033647, PubMed:21317537, PubMed:23542070). Interacts with RRM1; in a phosphorylation- and (dATP)-dependent manner. Interacts (via PEST domain when phosphorylated) with SLC4A4 isoform 1 but not isoform 2; the interaction increases SLC4A4 isoform 1 activity (PubMed:16769890, PubMed:19033647, PubMed:21317537). Interacts (when phosphorylated) with SLC9A3; the interaction is required for SLC9A3 apical location and activity (PubMed:19224921). Interacts (when phosphorylated) with FIP1L1; the interaction is direct and associates AHCYL1 with the CPSF complex and RNA Interacts with PAPOLA (By similarity). Interacts with ZCCHC4 (By similarity). Interacts with AHCY (By similarity). - Sub-Chronic Neuropathological and Biochemical Changes in Mouse Visual System after Repetitive Mild Traumatic Brain Injury.
Tzekov, PloS one 2016 - “...P01029 13 0.216 24.10% ribosomal protein S3 RPS3 P62908 14 0.234 26.40% adenosylhomocysteinase-like 1 AHCYL1 Q80SW1 15 0.234 26.40% peptidylprolyl isomerase B (cyclophilin B) PPIB P24369 16 0.254 28.90% filamin C, gamma FLNC Q8VHX6-2 17 0.257 29.30% quinoid dihydropteridine reductase QDPR Q8BVI4 18 0.276 31.80% glutamate-ammonia...”
- A fine-tuned β-catenin regulation during proliferation of corneal endothelial cells revealed using proteomics analysis.
Maurizi, Scientific reports 2020 - “...Protein symbol Uniprot p value Down-regulated in rCEnC Amino-acid biosynthesis S-adenosylhomocysteine hydrolase-like protein 1 AHCYL1 G1SXT1 <0.005 Cotransporter regulator Adenosylhomocysteinase 3 AHCYL2 G1T1W6 <0.001 Sodium pump Sodium/potassium-transporting ATPase subunit beta-1 ATP1B1 Q9TT37 <0.005 calcium homeostasis Plasma membrane calcium-transporting ATPase 4 ATP2B4 G1SR77 <0.005 Glycosylation Beta-1,3-glucosyltransferase B3GLCT...”
- Synaptic plasticity in hippocampal CA1 neurons of mice lacking inositol-1,4,5-trisphosphate receptor-binding protein released with IP3 (IRBIT).
Goto, Learning & memory (Cold Spring Harbor, N.Y.) 2022 - GeneRIF: Synaptic plasticity in hippocampal CA1 neurons of mice lacking inositol-1,4,5-trisphosphate receptor-binding protein released with IP3 (IRBIT).
- Both IRBIT and long-IRBIT bind to and coordinately regulate Cl-/HCO3- exchanger AE2 activity through modulating the lysosomal degradation of AE2.
Itoh, Scientific reports 2021 - GeneRIF: Both IRBIT and long-IRBIT bind to and coordinately regulate Cl(-)/HCO3(-) exchanger AE2 activity through modulating the lysosomal degradation of AE2.
- Modulation of Cl- signaling and ion transport by recruitment of kinases and phosphatases mediated by the regulatory protein IRBIT.
Vachel, Science signaling 2018 - GeneRIF: IRBIT controlled five phosphorylation sites in NBCe1-B that determined both the active conformation of the transporter and its regulation by Cl(-)
- The NHERF1 PDZ1 domain and IRBIT interact and mediate the activation of Na+/H+ exchanger 3 by ANG II.
He, American journal of physiology. Renal physiology 2016 - GeneRIF: NHERF1 mediates ANG II-induced activation of renal NHE3, which requires coordination between IRBIT and the NHERF1 PDZ1 domain in binding and transporting NHE3
- IRBIT regulates CaMKIIα activity and contributes to catecholamine homeostasis through tyrosine hydroxylase phosphorylation.
Kawaai, Proceedings of the National Academy of Sciences of the United States of America 2015 - GeneRIF: IRBIT suppresses CaMKIIalpha activity and contributes to catecholamine homeostasis through TH phosphorylation.
- IRBIT Interacts with the Catalytic Core of Phosphatidylinositol Phosphate Kinase Type Iα and IIα through Conserved Catalytic Aspartate Residues.
Ando, PloS one 2015 - GeneRIF: IRBIT forms signaling complexes with PIPKIalpha and NBCe1-B, whose activity is regulated by PI(4,5)P2.
- Irbit mediates synergy between ca(2+) and cAMP signaling pathways during epithelial transport in mice.
Park, Gastroenterology 2013 - GeneRIF: Irbit was sequestered by InsP3 receptors (IP3Rs) in the endoplasmic reticulum
- IRBIT governs epithelial secretion in mice by antagonizing the WNK/SPAK kinase pathway.
Yang, The Journal of clinical investigation 2011 - GeneRIF: IRBIT opposes the effects of WNKs and SPAK by recruiting PP1 to the complex to dephosphorylate CFTR and NBCe1-B, restoring their cell surface expression, in addition to stimulating their activities
- More
- A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites.
Mishra, Life science alliance 2024 - “...silencing 10 UTP3 Q9NQZ2 37.2 611614 CG9977 Q9VZX9 106749/KK ++ 36193/GD ++ Adenosylhomocysteinase-like 1 AHCYL1 O43865 72.87 607826 CG32847 Q8IQM1 104294/KK ++ 48423/GD ++ UN, contains the RING (Really Interesting New Gene) finger domain TRIM26 Q12899 21.25 600830 CG7839 Q9VTE6 105979/KK + 12691/GD + UN, orthologous...”
- Longitudinal Assessment of Nasopharyngeal Biomarkers Post-COVID-19: Unveiling Persistent Markers and Severity Correlations.
Redondo-Calvo, Journal of proteome research 2024 - “...protein ECM29 1.95 2.00 ECPAS Q5VYK3 SAHH2 S -adenosylhomocysteine hydrolase-like protein 1 1.82 2.02 AHCYL1 O43865 NFKB2* nuclear factor NF-kappa-B p100 subunit 2.07 2.02 NFKB2 Q00653 EIF3H eukaryotic translation initiation factor 3 subunit H 3.23 2.03 EIF3H O15372 IF16 -interferon-ind1ble protein 16 1.84 2.03 IFI16 Q16666...”
- Screening differentially expressed proteins to distinguish thymoma (B1 and B3) from thymic cysts based on tandem mass tag (TMT) technology.
Shi, Journal of cardiothoracic surgery 2024 - “...EPHX4 DOWN 0.003095 P78552 IL13RA1 DOWN 0.003324 Q8TBY8 PMFBP1 UP 0.003829 Q96H15 TIMD4 DOWN 0.006676 O43865 AHCYL1 DOWN 0.007515 P06312 IGKV4-1 UP 0.01023 Q08380 LGALS3BP UP 0.016919 P14314 PRKCSH DOWN 0.017522 Q13790 APOF UP 0.018709 P00740 F9 DOWN 0.019351 B3 vs. B1 P26572 MGAT1 DOWN 0.005284316...”
- The mitotic surveillance pathway requires PLK1-dependent 53BP1 displacement from kinetochores
Burigotto, 2023 - c-Abl Phosphorylates MFN2 to Regulate Mitochondrial Morphology in Cells under Endoplasmic Reticulum and Oxidative Stress, Impacting Cell Survival and Neurodegeneration.
Martinez, Antioxidants (Basel, Switzerland) 2023 - “...6.420 Q14318 FKBP8 Y187 GPQGRSPYIPPHAAL 9.852 0.627 6.177 Q8NAN2 MIGA1 Y152 KGSQVCNYANGGLFS 12.228 0.333 4.072 O43865 SAHH2 Y28 EIEDAEKYSFMATVT 9.989 0.347 3.466 Q07817 B2CL1 Y120 HITPGTAYQSFEQVV 9.800 0.167 1.637 O75323 NIPS2 Y264 GWEELVYYTVPLIQE 11.294 0.020 0.226 O43236 SEPT4 Y228 CWKPVAEYIDQQFEQ 10.305 N/A N/A O43236 SEPT4 Y407...”
- Mechanistic Effects of Baicalein on Aqueous Humor Drainage and Intraocular Pressure
Li, International journal of molecular sciences 2022 - “...0.5 * DAB2 Disabled homolog 2 P98082 0.7 0.5 * AHCYL1 S-adenosylhomocysteine hydrolase-like protein 1 O43865 1.3 0.3 * TUFM Elongation factor Tu, mitochondrial P49411 1.3 0.3 * ATP5PO ATP synthase subunit O, mitochondrial P48047 1.3 0.4 * EML4 Echinoderm microtubule-associated protein-like 4 Q9HC35 1.3 0.4...”
- Molecular Profiles of Amyloid-β Proteoforms in Typical and Rapidly Progressive Alzheimer's Disease
Noor, Molecular neurobiology 2022 - “...R Actin-related protein 2, 3 P61160 Nu, Cysk Cysk organization/Axon growth C, R Adenosylhomocysteinase 2 O43865 ER Translation C Adenylate kinase isoenzyme P00568 Cy Energy metabolism R ADP/ATP translocase 1, 2 P12235 Mit Energy metabolism C, R Band 4.1-like protein 3 Q9Y2J2 Mem, Cysk Apoptosis, Cysk...”
- Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia.
García-Berrocoso, Molecular & cellular proteomics : MCP 2018 - More
- RyR2/IRBIT regulates insulin gene transcript, insulin content, and secretion in the insulinoma cell line INS-1.
Harvey, Scientific reports 2022 - GeneRIF: RyR2/IRBIT regulates insulin gene transcript, insulin content, and secretion in the insulinoma cell line INS-1.
- The IP3 R Binding Protein Released With Inositol 1,4,5-Trisphosphate Is Expressed in Rodent Reproductive Tissue and Spermatozoa.
Borth, Journal of cellular physiology 2016 (PubMed)- GeneRIF: The present study documents that IRBIT is expressed in Leydig and Sertoli cells.
- IRBIT Interacts with the Catalytic Core of Phosphatidylinositol Phosphate Kinase Type Iα and IIα through Conserved Catalytic Aspartate Residues.
Ando, PloS one 2015 - GeneRIF: IRBIT forms signaling complexes with PIPKIalpha and NBCe1-B, whose activity is regulated by PI(4,5)P2.
- Activation of Na+/H+ exchanger NHE3 by angiotensin II is mediated by inositol 1,4,5-triphosphate (IP3) receptor-binding protein released with IP3 (IRBIT) and Ca2+/calmodulin-dependent protein kinase II.
He, The Journal of biological chemistry 2010 - GeneRIF: IRBIT is critically involved in mediating activation of NHE3 by ANG II via a Ca(2+)/calmodulin-dependent protein kinases II-dependent pathway.
SAHH2_RAT / B5DFN2 S-adenosylhomocysteine hydrolase-like protein 1; IP3R-binding protein released with inositol 1,4,5-trisphosphate; Putative adenosylhomocysteinase 2; S-adenosyl-L-homocysteine hydrolase 2; AdoHcyase 2 from Rattus norvegicus (Rat) (see paper)
48% identity, 95% coverage
- function: Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate, competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. Promotes the formation of contact points between the endoplasmic reticulum (ER) and mitochondria, facilitating transfer of Ca(2+) from the ER to mitochondria (By similarity). Under normal cellular conditions, functions cooperatively with BCL2L10 to limit ITPR1- mediated Ca(2+) release but, under apoptotic stress conditions, dephosphorylated which promotes dissociation of both AHCYL1 and BCL2L10 from mitochondria-associated endoplasmic reticulum membranes, inhibits BCL2L10 interaction with ITPR1 and leads to increased Ca(2+) transfer to mitochondria which promotes apoptosis (By similarity). In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (By similarity). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates from ITPR1 to interact with CFTR and SLC26A6, mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (By similarity). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed:20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition. May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state. Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (By similarity). In vitro does not exhibit any S-adenosyl-L-homocysteine hydrolase activity (By similarity).
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Forms multimers (By similarity). Forms heteromultimers with AHCYL2 (via the C-terminal region). Interacts (when phosphorylated) with ITPR1 (when not phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1 (By similarity). Interacts with BCL2L10; this strengthens the interaction of AHCYL1 with ITPR1 (By similarity). Interacts with CFTR and SLC26A6; the interactions take place once AHCYL1 is released from ITPR1 and increase CFTR and SLC26A6 activities (By similarity). Interacts with RRM1; in a phosphorylation- and (dATP)-dependent manner. Interacts (via PEST domain when phosphorylated) with SLC4A4 isoform 1 but not isoform 2; the interaction increases SLC4A4 isoform 1 activity. Interacts (when phosphorylated) with SLC9A3; the interaction is required for SLC9A3 apical location and activity (PubMed:20584908). Interacts (when phosphorylated) with FIP1L1; the interaction is direct and associates AHCYL1 with the CPSF complex and RNA. Interacts with PAPOLA (By similarity). Interacts with ZCCHC4 (By similarity). Interacts with AHCY (By similarity). - Proteomic analysis of rat colonic mucosa following acupuncture treatment for irritable bowel syndrome with diarrhea
Liu, PloS one 2022 - “...Uniprot accession Protein name Gene M vs C SD vs M FC P-value FC P-value B5DFN2 Adenosylhomocysteinase Ahcyl1 3.2 0.034 -2.3 0.045 P62744 AP-2 complex subunit sigma Ap2s1 2.0 0.013 -2.3 0.033 P14173 Aromatic-L-amino-acid decarboxylase Ddc 1.9 0.022 -1.8 0.017 Q9Z1N4 3(2),5-bisphosphate nucleotidase 1 Bpnt1 1.9...”
- “...EH domain-containing protein 3 Ehd3 1.2 0.043 P11661 NADH-ubiquinone oxidoreductase chain 5 Mtnd5 -3.0 0.048 B5DFN2 Adenosylhomocysteinase Ahcyl1 -2.3 0.045 P62744 AP-2 complex subunit sigma Ap2s1 -2.3 0.033 P62278 40S ribosomal protein S13 Rps13 -2.0 0.041 P14173 Aromatic-L-amino-acid decarboxylase Ddc -1.8 0.017 P46413 Glutathione synthetase Gss...”
A2E342 Adenosylhomocysteinase from Trichomonas vaginalis (strain ATCC PRA-98 / G3)
47% identity, 96% coverage
- Comparative proteomic analysis of metronidazole-sensitive and resistant Trichomonas vaginalis suggests a novel mode of metronidazole action and resistance
Mayr, International journal for parasitology. Drugs and drug resistance 2024 - “...putative A2G856 2 4.5 4.9 Dynein heavy chain family protein A2EGW8 2 5.9 5.9 Adenosylhomocysteinase A2E342 3,1 2.3 2.2 Inosine-uridine preferring nucleoside hydrolase A2DN71 3,1 7.3 6.6 V-type proton ATPase subunit A2FED9 5.3 6.1 6.8 V-type proton ATPase subunit A2E709 3.2 2.9 9.9 C2 domain containing...”
- “...two transmembrane proteins of unknown function (A2E8X1 and A2GRV0), an isoform of dynein (A2EGW8), adenosylhomocysteinase (A2E342), and a nucleoside hydrolase (A2DN71). Further work is necessary before speculating on the roles of these factors in the development of in vitro metronidazole resistance because the changes could be...”
P35007 Adenosylhomocysteinase from Catharanthus roseus
47% identity, 94% coverage
LOC100253872 adenosylhomocysteinase from Vitis vinifera
A5C5K3 Adenosylhomocysteinase from Vitis vinifera
48% identity, 94% coverage
- Annotation and re-sequencing of genes from de novo transcriptome assembly of Abies alba (Pinaceae)
Roschanski, Applications in plant sciences 2013 - “...GTTCCAAGCTTCCACAATACTC 2565 F: GTGTCTGGAAGGGAATACAAGG 58 0 432 1 PREDICTED: Vitis vinifera adenosylhomocysteinase-like, transcript variant 1 (LOC100253872), mRNA (1E-109) embryonic development ending in seed dormancy, one-carbon metabolic process, posttranscriptional gene silencing, methylation-dependent chromatin silencing R: CCTTGACTCCTTCATGGATCAG 2774 F: GTTACAGGAAGCCTTTCTGG 55 0 502 2 Citrus sinensis pectinesterase mRNA,...”
- Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress
Prinsi, International journal of molecular sciences 2020 - “...N and amino acid metabolism (12, 13) 231 F6HQA7 Nitrite reductase 1 (12) 4.11 25 A5C5K3 Adenosyl homocysteinase (13) 1.43 139 A5CAL1 Glyoxylate/hydroxypyruvate reductase A HPR2 (13, 1, 26) 1.45 155 A5ATW2 Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase (13) 1.61 26 P51119 Glutamine synthetase cytosolic isozyme 2 (12)...”
N1QRC7 Adenosylhomocysteinase from Aegilops tauschii
47% identity, 94% coverage
A0A075TMT0 adenosylhomocysteinase (EC 3.3.1.1) from Populus tomentosa (see paper)
47% identity, 94% coverage
P50249 Adenosylhomocysteinase from Phalaenopsis sp.
47% identity, 94% coverage
DVU0607 adenosylhomocysteinase from Desulfovibrio vulgaris Hildenborough
49% identity, 96% coverage
- Towards a rigorous network of protein-protein interactions of the model sulfate reducer Desulfovibrio vulgaris Hildenborough
Chhabra, PloS one 2011 - “...partners of Strep -tagged ApsA. These included the S-adenosylmethionine synthetase (MetK, DVU2449) and adenosylhomocysteinase (AhcY, DVU0607) ( Table S12 ). Using Strep -tagged ApsB, we observed the larger subunit, ApsA, as an interacting partner, but none of the other enzymes involved in the sulfate reduction pathway...”
- Analysis of a ferric uptake regulator (Fur) mutant of Desulfovibrio vulgaris Hildenborough
Bender, Applied and environmental microbiology 2007 - “...1 DVU2441 DVU2442 2 DVU2494 V. Iron induced 2 DVU0186 2 DVU0607 2 DVU0997 2 DVU1257 2 DVU3371 WT, 5/WT, 60 Fur, 5/Fur, 60 3.8 3.5 0.3 0.2 1.6 2.5 1.9 1.3...”
GRMZM2G015295, NP_001148534 adenosylhomocysteinase from Zea mays
C0PHR4 Adenosylhomocysteinase from Zea mays
47% identity, 94% coverage
- Comparative Transcriptome Analysis of Iron and Zinc Deficiency in Maize (Zea mays L.)
Mallikarjuna, Plants (Basel, Switzerland) 2020 - “...pathway genes in maize seedlings ( Figure S3 ). S-adenosyl-L-homocysteine hydrolysing enzyme ADENOSYL HOMOCYSTEINASE ( GRMZM2G015295; Fe: 7.51-fold and FeZn: 30.81-fold) showed the upregulated expression under Fe deficiency in the shoot. O-methyltransferases mediate the conversion of homocysteine to methionine and it serves as a precursor for...”
- “...universal phenomenon ( Figure 7 ; Table S3 ). For instance, six transporter genes ( GRMZM2G015295 , GRMZM2G067546 , GRMZM2G070605 , GRMZM2G099340 , GRMZM2G099628 , GRMZM2G128995 ) showing Fe specific upregulation showed a common TF-mediated regulation through EREB142 (GRMZM2G010100). Conversely, many of the transporter genes expression...”
- Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition
Li, Molecules (Basel, Switzerland) 2019 - “...93,569 166 7 5.90 4 Ascorbate peroxidase ACO90192 250 27,597 78 8 8.00 5 Adenosylhomocysteinase NP_001148534 485 53,248 349 22 22.06 6 APx3-Peroxisomal ascorbate peroxidase NP_001148710 290 32,072 67 7 2.76 7 Uncharacterized LOC100216603 NP_001336786 129 14,353 45 3 15.50 8 60S Ribosomal protein L37a-2 ONM18155...”
- ZmSTK1 and ZmSTK2, encoding receptor-like cytoplasmic kinase, are involved in maize pollen development with additive effect
Fan, Plant biotechnology journal 2018 - “...the components of these 55kDa protein complexes were identified, including enolase1 (NP_001105896), enolase2 (NP_001105371), adenosylhomocysteinase (NP_001148534), reticuline oxidase precursor (NP_001148634), elongation factor 1alpha (NP_001151074), catalase isozyme B (NP_001241808) and exopolygalacturonase (AFW80846). Second, Y2H was employed to confirm the interaction proteins among the seven proteins with ZmSTK1...”
- Correlation analysis of the transcriptome of growing leaves with mature leaf parameters in a maize RIL population
Baute, Genome biology 2015 - “...grows slower, plants are darker green and leaves are more narrow [ 103 ] Unknown GRMZM2G015295 Adenosylhomo-cysteinase AT3G23810 b,c SAHH2, EMB1395HOG1, MEE58, SAHH1, S-adenosyl-l-homocysteine (SAH) hydrolase 2, S-adenosyl-L-homocysteine hydrolase AT4G13940 a,b,c Loss of function mutants show increased leaf size, higher seed yields and delayed flowering, while...”
- “...which shows homology to the auxin efflux carriers or PINs [ 77 , 104 ], GRMZM2G015295 , a S-adenosyl-L-homocysteine hydrolase showing homology to cytokinin binding protein HOG1 [ 105 ] and GRMZM2G702026 , a homolog of AUXIN RESPONSE FACTOR 1 ( ARF1 ) [ 77 ]....”
- iTRAQ-Based Proteomic Analysis Reveals Several Strategies to Cope with Drought Stress in Maize Seedlings
Jiang, International journal of molecular sciences 2019 - “...1 0.51840625 Vacuolar protein sorting-associated protein 29 B8A390 10.5 4 0.6845 Vacuolar-type H+-pyrophosphatase5 Transcription Related C0PHR4 58.4 25 0.74545 Adenosyl homocysteinase A0A1D6HZB8 18.3 3 1.55415 Alkyl transferase K7TI82 2 1 1.3210625 C3H transcription factor B6SSH9 57.1 30 1.6191 Extracellular ribonuclease LE OS=Zea mays PE=2 SV=1 A0A1D6J4Q0...”
- Proteomic profiling of maize opaque endosperm mutants reveals selective accumulation of lysine-enriched proteins
Morton, Journal of experimental botany 2016 - “...Fructose-bisphosphate aldolase 9.88E06 8.45% 853.65 1313.69 11.68 B4FT23 14-3-3-like protein 1.05E05 7.54% 934.66 1551.91 10.06 C0PHR4 Adenosylhomocysteinase 1.69E04 7.63% 393.92 933.63 9.28 Down_ o2 e B6SIX6 Prolamin PPROL 17 6.02E09 0.56% 198.48 6.89 10.25 P04698 Zein- PZ22.3 2.99E10 0.37% 192.40 23.41 9.36 B6SI09 Aquaporin TIP3.1 5.57E06...”
SAHH1_ARATH / O23255 Adenosylhomocysteinase 1; AdoHcyase 1; Protein EMBRYO DEFECTIVE 1395; Protein HOMOLOGY-DEPENDENT GENE SILENCING 1; S-adenosyl-L-homocysteine hydrolase 1; SAH hydrolase 1; EC 3.13.2.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
NP_193130 S-adenosyl-L-homocysteine hydrolase from Arabidopsis thaliana
46% identity, 94% coverage
- function: Essential protein during embryogenesis (PubMed:15266054). Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L- methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine (PubMed:15659630). Required for DNA methylation- dependent gene silencing (PubMed:15659630).
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Homotetramer.
disruption phenotype: Embryo defective arrested at the globular stage (PubMed:15266054). Null mutations are homozygous lethal (PubMed:15266054). - Water deficit affected flavonoid accumulation by regulating hormone metabolism in Scutellaria baicalensis Georgi roots
Yuan, PloS one 2012 - “...JX068531 009 XP_002313027 BEL1-related homeotic protein, putative ns 1.192 1.731 78 9 21 JX068534 014 NP_193130 Adenosylhomocysteinase/copper ion binding ns 1.969 ns 88 10 23 JX068535 015 ACJ24804 Chloroplast heat shock protein 70B ns 1.258 ns 75 7 73 JX068536 020 AAU84988 Anthranilate synthase alpha 1...”
- Nuclear targeting of methyl-recycling enzymes in Arabidopsis thaliana is mediated by specific protein interactions.
Lee, Molecular plant 2012 (PubMed)- GeneRIF: The nuclear targeting of ADK1 and SAHH1 is mediated by their interaction with cap MT.
- Transcriptome profiling and methyl homeostasis of an Arabidopsis mutant deficient in S-adenosylhomocysteine hydrolase1 (SAHH1).
Ouyang, Plant molecular biology 2012 (PubMed)- GeneRIF: SAHH1 plays a critical role in methyl homeostasis, and its deficiency is a major contributing factor to the change of global gene expression, metabolic pathways and activation of transposable elements in the sahh1 mutant.
- Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters.
Godge, Plant cell reports 2008 (PubMed)- GeneRIF: HOG1 has high affinity cytokinin binding activity. Overexpression led to early flowering with a significantly reduced plant biomass and number of leaves.
- A histone methylation-dependent DNA methylation pathway is uniquely impaired by deficiency in Arabidopsis S-adenosylhomocysteine hydrolase.
Mull, Genetics 2006 - GeneRIF: A histone methylation-dependent DNA methylation pathway is uniquely impaired by deficiency in adenosylhomocysteinase.
- Transcriptomic analyses in the gametophytes of the apomictic fern Dryopteris affinis
Ojosnegros, Planta 2024 - “...1 121247-224 O23676 MEE63 MATERNAL EFFECT EMBRYO ARREST 63 17 150 110 91 1 13540-658 O23255 MEE58 MATERNAL EFFECT EMBRYO ARREST 58 53 485 0 3 250508-99 Flowering Q0WP44 HST HASTY 133 1202 0 2 367437-36 Q8W234 SEU SEUSS 96 877 710 99 3 42405-391 O23624...”
- Epigenetic variation in early and late flowering plants of the rubber-producing Russian dandelion Taraxacum koksaghyz provides insights into the regulation of flowering time
Roelfs, Scientific reports 2024 - “...on loci possessing DNA methylation but lacking H3K4me2/me3 129 , 130 TkA01G206370 0.35 CHH Upstream O23255 93.20 Adenosylhomocysteinase 1 SAHH1-like DNA methylation and histone modification Hydrolysis of S-adenosyl-homocysteine formed as a by-product of and competitively inhibiting methyltransferase reactions 131 , 132 0.4 TkA02G027650 0.35 CHH Upstream...”
- The Shared Proteome of the Apomictic Fern Dryopteris affinis ssp. affinis and Its Sexual Relative Dryopteris oreades
Ojosnegros, International journal of molecular sciences 2022 - “...107 Gene expression tr|A8J568|A8J568_CHLRE Q9FMR9 RIN1 RuvB-like protein 1 164 1.104 0 Gene expression 239719-107_1_ORF2 O23255 HOG1 Adenosylhomocysteinase 1 48 485 0 Gene expression tr|B9RUZ2|B9RUZ2_RICCO Q9C944 HTA9 Histone H2A.Z variant 14 134 6.67 10 76 Biotic stress 24640-505_5_ORF2 Q940H6 OST1 Serine/threonine-protein kinase SRK2E 41 362 0...”
- Profiling of advanced glycation end products uncovers abiotic stress-specific target proteins in Arabidopsis
Chaplin, Journal of experimental botany 2019 - “...8 EMBRYO DEFECTIVE 1395,HOMOLOGY-DEPENDENT GENE SILENCING 1, MATERNAL EFFECT EMBRYO ARREST 58, S-ADENOSYL-L-HOMOCYSTEIN HYDROLASE 1, O23255 AT4G13940 8 - 9 TRANSLATIONALLY CONTROLLED TUMOR PROTEIN P31265 AT3G16640 5 - 8 BETA CARBONIC ANHYDRASE 2 P42737 AT5G14740 8 ASCORBATE PEROXIDASE 1 Q05431 AT1G07890 8 - 12 THIAMINE4 Q38814...”
- How haptophytes microalgae mitigate vitamin B12 limitation
Nef, Scientific reports 2019 - “...Escherichia coli (P0A817) and Arabidopsis thaliana (Q9SJL8), Homo sapiens and A . thaliana SAHH (P23526; O23255), Rattus norvegicus CBLA (D3ZNY3), Homo sapiens CBLB (Q96EY8) and MMCM (P22033), Propionibacterium freudenreichii subsp. shermanii MMCM (P11653). Homologous genes identified this way were searched again in T . lutea genome...”
- Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions
Wang, Scientific reports 2017 - “...protease proteolytic subunit Oenothera hookeri P56772 clpP 3 0.50 0.00263 16 P68173 Adenosylhomocysteinase Nicotiana tabacum O23255 SAHH 2 2.13 2.7E-06 17 Q9497 Diaminopimelate decarboxylase 1, chloroplastic Arabidopsis thaliana Q9497 LYSA1 4 0.52 3.9E-05 18 Q940P8 T-complex protein 1 subunit beta Arabidopsis thaliana Q940P8 CCT2 2 0.21...”
- Leaf apoplastic proteome composition in UV-B treated Arabidopsis thaliana mutants impaired in extracellular glutathione degradation
Masi, Data in brief 2016 - “...0.68 Probable pectate lyase 18 12.3 3 Q940J8 At4g19410 0.68 Pectinacetylesterase family protein 63.2 21 O23255 At4g13940 1.5 Adenosylhomocysteinase 1 24.74 12 F4JRV2 At4g25100 1.7 Superoxide dismutase 12.4 3 F4JBY2 At3g60750 2.2 Transketolase 29.1 17 O50008 At5g17920 2.4 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase 36.21 28 Table 3 Expression change...”
- Proteome Scale-Protein Turnover Analysis Using High Resolution Mass Spectrometric Data from Stable-Isotope Labeled Plants
Fan, Journal of proteome research 2016 - “...At3g15950 8 5.45 0.15 0.03 30.30 Q9SYT0 Annexin D1 At1g35720 7 5.26 0.08 0.02 26.49 O23255 Adenosylhomocysteinase 1 At4g13940 6 5.46 0.13 0.02 30.48 Q9LF98 Fructose-bisphosphate aldolase At3g52930 6 5.40 0.11 0.02 29.30 O50008 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 1 At5g17920 5 5.10 0.11 0.02 23.69 Q9XEE2 Annexin D2...”
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Q84RD8 Adenosylhomocysteinase from Medicago truncatula
47% identity, 96% coverage
P50246 Adenosylhomocysteinase from Medicago sativa
47% identity, 96% coverage
- Digestomic data of proteolysis during whether post rumen digestion after tannin supplementation
Chambon, Data in brief 2022 - “...psbA 15 14 905.08 Probable aquaporin TIP-type 25324.4 P42067 MCP1 5 5 322.39 Adenosylhomocysteinase 53744.0 P50246 SAHH 2 2 69.84 Actin-7 41913.0 XP_003602545 actin2 10 9 572.66 Photosystem II 47 kDa protein 56131.0 YP_001381703 psbB 22 22 1360.88 Photosystem II protein V 9400.6 YP_001381711 psbE 2...”
- Drought Stress Induces Morpho-Physiological and Proteome Changes of Pandanus amaryllifolius.
Amnan, Plants (Basel, Switzerland) 2022 - “...cyclase, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3 Q9CA67 Geranylgeranyl diphosphate reductase, chloroplastic Chlorophyll biosynthesis Oxidoreductase 3 P50246 Adenosylhomocysteinase One-carbon metabolism Hydrolase 3 Q6ZIV7 Hypersensitive-induced response protein 1 Potassium ion channel regulation Histidine kinase binding 3 Q9SI75 Elongation factor G, chloroplastic Protein biosynthesis Elongation factor 3 P24846 4-hydroxy-tetrahydrodipicolinate...”
L0AUI8 adenosylhomocysteinase (EC 3.3.1.1) from Populus tomentosa (see paper)
47% identity, 94% coverage
Q84VE1 Adenosylhomocysteinase from Oryza sativa subsp. japonica
47% identity, 94% coverage
- Differential Proteomic Analysis Using iTRAQ Reveals Alterations in Hull Development in Rice (Oryza sativa L.)
Wang, PloS one 2015 - “...fermentation to lactate pathway, S-adenosylmethionine synthase 2 (SAM2; P93438) in the S-adenosyl-L-methionine biosynthesis pathway, adenosylhomocysteinase (Q84VE1) in the L-homocysteine biosynthesis pathway, and chalcone synthase 1 (CHS1; Q2R3A1) in flavonoid biosynthesis pathway were all down-regulated in the flowering and milk-ripe stages. Three significantly differentially expressed proteins were...”
- “...Q0E4Q5 Oxylipin biosynthesis A2XL22 A2XL22 sucrose biosynthesis Q0JGK4 Q0JGK4 Q0JGK4 S-adenosyl-L-methionine biosynthesis P93438 L-homocysteine biosynthesis Q84VE1 urea degradation E0ZS48 E0ZS48 protoporphyrin-IX biosynthesis Q10LR9 tetrahydrofolate interconversion Q7Y1F0;Q10BJ7 Q7Y1F0 Q10BJ7 flavonoid biosynthesis Q2R3A1 glutathione biosynthesis Q6Z3A3 Q6Z3A3 pentose phosphate pathway Q10M94 Q10M94 Glycolysis Q6K5G8;Q7XKB5;Q7FAH2;B8ACJ0 Q6K5G8 Q6K5G8 AMP biosynthesis...”
Q84RD9 Adenosylhomocysteinase from Medicago truncatula
47% identity, 94% coverage
SAHH3_DROME / P50245 Adenosylhomocysteinase-like 2; dAhcyL2; Inactive S-adenosyl-L-homocysteine hydrolase 2; S-adenosylhomocysteine hydrolase-like protein 2 from Drosophila melanogaster (Fruit fly) (see 3 papers)
48% identity, 94% coverage
- function: Might play a role in the regulation of methionine metabolism.
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
disruption phenotype: RNAi-mediated knockdown results in extended lifespan with increased fecundity and suppression of age-related decreased climbing activity and intestinal integrity (PubMed:27313316). Does not affect oxidative stress resistance (PubMed:27313316). RNAi- mediated knockdown in fat body or neurons does not show any phenotype (PubMed:27313316). Simultaneous knockdown of AhcyL1 in intestine results in extended life span (PubMed:27313316).
A8IXE0 Adenosylhomocysteinase from Chlamydomonas reinhardtii
46% identity, 95% coverage
P32112 Adenosylhomocysteinase from Triticum aestivum
46% identity, 94% coverage
- H2 O2 , NO, and H2 S networks during root development and signalling under physiological and challenging environments: Beneficial or toxic?
Mukherjee, Plant, cell & environment 2023 - “...et al. ( 2021 ) UDPglycosyltransferase Q9FIA0 Persulfidation JuradoFlores et al. ( 2021 ) Adenosylhomocysteinase P32112 Snitrosation Jain et al. ( 2018 ) Sadenosylmethionine synthetase Q9LUT2 Snitrosation Ssulfenylation Persulfidation JuradoFlores et al. ( 2021 ); Lindermayr et al. ( 2005 ); Wei et al. ( 2020...”
- The TriMet_DB: A Manually Curated Database of the Metabolic Proteins of Triticum aestivum
Cunsolo, Nutrients 2022 - “...Swiss-Prot_DB, seven of them (i.e., the entries with Acc. No. F8RP11, P12782, O64393, P93594, P02277, P32112, and P62786) were not listed in the final report of the TriMet_DB search, because they did not satisfy one of the two requirements aforementioned. Particularly, the F8RP11 entry (Hsp70-Hsp90 organizing...”
- “...and, therefore, did not satisfy the first requirement. The other five proteins (O64393, P93594, P02277, P32112, and P62786) instead represent a classical example of intersection protein (see Figure 4 , case b), not supported by unique peptides. As an example, Figure 6 shows the Occams razor...”
- Aluminum Stress Induces Irreversible Proteomic Changes in the Roots of the Sensitive but Not the Tolerant Genotype of Triticale Seedlings
Niedziela, Plants (Basel, Switzerland) 2022 - “...2 ATP synthase subunit alpha, mitochondrial P12862 201/96 55,515 6.6 4 +3.54 +2.73 3 Adenosylhomocysteinase P32112 34 54,086 7.85 1 2.00 0.83 4 Phosphoglycerate kinase, cytosolic P12783 372 42,153 5.6 7 +3.13 +5.00 5 Fructose-1,6-bisphosphatase P09195 24 44,703 7.1 1 +2.03 * n.s. Metabolic pathway/Flavonoid metabolism...”
- “...O-methyltransferase 1 Q84N28 1053 39,177 5.7 22 see Table 2 +0.26 Methyl cycle 32 Adenosylhomocysteinase P32112 137 54,086 6.8 3 0.20 n.s. Protease inhibitor 33 Ubiquitin P69326 67 8648 7.6 1 +0.21 n.s. 34 Ubiquitin P69326 70 8648 6.45 1 +0.22 n.s. 35 Ubiquitin P69326 40...”
- Comparative protein profiles of the Ambrosia plants.
Barton, Biochimica et biophysica acta. Proteins and proteomics 2017 - “...thaliana Q42560 98153 4 100 18 20 1 1 1 Citrate Metabolism Adenosylhomocysteinase T. aestivum P32112 53438 2 100 10 18 1 1 One Carbon Metabolism Adenylate kinase A O. sativa.jap Q08479 26407 2 100 2 10 1 Nucleotide Metabolism Alpha-1,4-glucan-protein synthase [UDP-forming] Z. mays P80607...”
- Harvest index, a parameter conditioning responsiveness of wheat plants to elevated CO2
Aranjuelo, Journal of experimental botany 2013 - “.../B Elev 0.1 METABOLISM 956 P50249 Adenosylhomocysteinase 19.3 53.14 5.89 3 1.37 1.32 1.8 1093 P32112 Adenosylhomocysteinase 89.2 53.44 5.74 12 1.44 1.35 766 O50008 Homocysteine methyltransferase 9.8 84.85 6.19 1 1.34 1.19 1330 P13564 Glutamine synthetase 79.8 47.09 5.18 12 1.43 1332 P13564 Glutamine synthetase...”
D2W1E4 Adenosylhomocysteinase from Naegleria gruberi
46% identity, 99% coverage
5v96A / A0A1Z0YU84 Crystal structure of s-adenosyl-l-homocysteine hydrolase from naegleria fowleri with bound NAD and adenosine
46% identity, 98% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (5v96A)
F0JE79 Adenosylhomocysteinase from Pseudodesulfovibrio mercurii
48% identity, 99% coverage
Q9SWF5 Adenosylhomocysteinase from Solanum lycopersicum
46% identity, 94% coverage
- Allergen false-detection using official bioinformatic algorithms.
Herman, GM crops & food 2020 - “...Q02060 Rice Tomato Wheat Q6Z782 P14903 P33432 2 A2XMB2 P38416 Q08000 P37833 Q08655 P20158 P46520 Q9SWF5 Q03968 2 Q07661 P10967 1 W5BUF4 P55142 P47921 P12299 P0C5A4 1 Q6QLX4 5 P02276 1 Q6AVA8 Q40128 P30523 Q69UI2 Q08451 P12783 Q01L47 2 Q43497 Q94JF2 P93207 Q10LP5 P05116 Q9AUV8 1...”
XP_009135865 adenosylhomocysteinase 2 from Brassica rapa
45% identity, 94% coverage
U6KN65 Adenosylhomocysteinase from Eimeria tenella
46% identity, 93% coverage
AT3G23810 SAHH2 (S-ADENOSYL-L-HOMOCYSTEINE (SAH) HYDROLASE 2); adenosylhomocysteinase/ binding / catalytic from Arabidopsis thaliana
46% identity, 94% coverage
- Assessing the Impacts of Cu and Mo Engineered Nanomaterials on Crop Plant Growth Using a Targeted Proteomics Approach
Li, ACS agricultural science & technology 2024 - “...pathway ID pathway protein ID accession number protein signature peptide A amino acid metabolism P1 AT3G23810 AA degradation methionine LVGVSEETTTGVK P2 AT5G17920 AA synthesis methionine GNATVPAMEMTK P3 AT1G02500 S-adenosylmethionine synthase FVIGGPHGDAGLTGR B fermentation P4 AT1G23800 aldehyde dehydrogenase VAEGDAEDVDRAVVAAR C glycolysis P5 AT2G36460 glycolysis cytosolic branch UGPase...”
- New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics
Arenas-Alfonseca, Plants (Basel, Switzerland) 2023 - “...ATPase, V0/A0 complex, subunit C/D AT3G28710 ATPase, V0/A0 complex, subunit C/D AT4G13940 ATSAHH1-HOG1 ATSAHH1__S-adenosyl-L-homocysteine hydrolase AT3G23810 ATSAHH2 ATSAHH2__S-adenosyl-l-homocysteine (SAH) hydrolase 2 AT3G48340 CEP2 CEP2__Cysteine proteinases superfamily protein AT2G42490 CuAO-zeta CuAO-zeta__Copper amine oxidase family protein AT3G53580 diaminopimelate epimerase family protein AT1G13950 ATELF5A ELF5A-1__eukaryotic elongation factor 5A-1 AT1G69410...”
- Role of NPR1 in Systemic Acquired Stomatal Immunity
Guan, Plants (Basel, Switzerland) 2023 - “...3.18 AT1G16720 High chlorophyll fluorescence phenotype 173 0.03 3.01 AT5G64050 Glutamate tRNA synthetase 0.01 3.01 AT3G23810 S-adenosyl-l-homocysteine hydrolase 2 0.00 2.96 AT1G79720 Eukaryotic aspartyl protease family protein 0.03 2.94 AT2G35040 AICARFT/IMPCHase bienzyme family protein 0.00 2.80 AT1G71720 Nucleic acid-binding proteins superfamily 0.02 2.77 AT3G46740 Translocon at...”
- Plant homocysteine, a methionine precursor and plant's hallmark of metabolic disorders
Sobieszczuk-Nowicka, Frontiers in plant science 2022 - “...( ZmCBL ); LOC_Os06g07860 ( OsCBL1 ); LOC_Os06g07960 ( OsCBL2 ); AT4G13940 ( AtSAHH1 ); AT3G23810 ( AtSAHH2 ); Glyma.11G254700 ( GmSAHH1 ); Glyma.05G152000 ( GmSAHH2 ); Glyma.08G108800 ( GmSAHH3 ); Potri.001G320500 ( PtSAHH1 ); Potri.017G059400 ( PtSAHH2 ); PGSC0003DMG400004572 ( StSAHH );VIT_205s0029g00330 ( VvSAHH1 );...”
- Evolutionary conservation and post-translational control of S-adenosyl-L-homocysteine hydrolase in land plants
Alegre, PloS one 2020 - “...cellular transmethylation potential. The Arabidopsis thaliana genome encodes two SAHH isoforms, SAHH1 (AT4G13940) and SAHH2 (AT3G23810) and particularly SAHH1 is indispensable for physiological functions at different developmental stages [ 6 8 ]. Null mutation of SAHH1 has been reported embryo lethal in A . thaliana and...”
- Co-silencing of tomato S-adenosylhomocysteine hydrolase genes confers increased immunity against Pseudomonas syringae pv. tomato DC3000 and enhanced tolerance to drought stress
Li, Frontiers in plant science 2015 - “...mutants. In Arabidopsis , there are two genes encoding for SAHHs, AtSAHH1 (At4g13940) and AtSAHH2 (At3g23810) ( Rocha et al., 2005 ; Pereira et al., 2007 ; Li et al., 2008 ). AtSAHH1 seems to be more important than AtSAHH2 because mutations in AtSAHH1 are embryonic...”
- Transcriptome-wide analysis of SAMe superfamily to novelty phosphoethanolamine N-methyltransferase copy in Lonicera japonica
Yuan, International journal of molecular sciences 2014 - “...SEPALLATA AT1G24260 195015 8.09 571982 0 SAHH Maintenance and recycling of S -adenosylmethionine dependent methylation AT3G23810 183400 7.06 569411 0 ADK AT3G09820 101959 323.33 388474 294.47 Abbreviations: AT, Arabidopsis thaliana ; FLJ, Lonicera japonica ; rFLJ, Lonicera japonica . var. chinensis (Watts.); RPKM, gene express RPKM;...”
- Primers for low-copy nuclear genes in the Hawaiian endemic Clermontia (Campanulaceae) and cross-amplification in Lobelioideae
Pillon, Applications in plant sciences 2013 - “...(acetylserine(thiol)lyase) TCTTATCGAACCAACAAGTGG TCGGATTGGCAGGATTCTC 257 no Clerm8 At5g05170 CESA3 (cellulose synthase) AGAGCCATCGCAATTGGCTG GCGAAATACCACTCTGGAGC 264 no Clerm9 At3g23810 SAHH2 ( S -adenosyl- l -homocysteine hydrolase) CATGTCCTTAGCCGACTTCG ACCATTAGCCTGCATTTGG 608 no Clerm10 At1g79550 PGK (phosphoglycerate kinase) GTCAAGATGGCAAATGATTGC CCTTCAGTGGAAGCATGAGC 212 yes Clerm11 At1g66580 SAG24 (senescence-associated gene/ribosomal protein L10e) GACCTGCTAGGTGTTACCGT CCCTCATACCAGTCTGGAGC 339...”
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1v8bA / P50250 Crystal structure of a hydrolase (see paper)
44% identity, 96% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (1v8bA)
SAHH_PLAF7 / P50250 Adenosylhomocysteinase; AdoHcyase; PfSAHH; S-adenosyl-L-homocysteine hydrolase; EC 3.13.2.1 from Plasmodium falciparum (isolate 3D7) (see paper)
P50250 adenosylhomocysteinase (EC 3.3.1.1) from Plasmodium falciparum (see 2 papers)
PF3D7_0520900, XP_001351767 adenosylhomocysteinase from Plasmodium falciparum 3D7
44% identity, 95% coverage
- function: Adenosylhomocysteine is a competitive inhibitor of S- adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine
catalytic activity: S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine (RHEA:21708)
cofactor: NAD(+) Note=Binds 1 NAD(+) per subunit
subunit: Homotetramer. - An optimized deep-forest algorithm using a modified differential evolution optimization algorithm: A case of host-pathogen protein-protein interaction prediction
Emmanuel, Computational and structural biotechnology journal 2025 - “...the predicted interactions. Also, the first 3 host-pathogen PPI in Table 2 , such as P50250 - P08319, Q8ILI6 - O94813, and Q7KQL3 - Q96GQ7, have been reported in the literature, while the remaining seven host-pathogen PPI are the novel interactions predicted by our model (in...”
- “...Predicted Human- Plasmodium falciparum (PF) protein-protein interaction. Table 3 Pathogen ID Annotation Host ID Annotation P50250 Adenosylhomocysteine P08319 All-trans-retinol dehydrogenase ADH4. Q8ILI6 Acidic leucine-rich nuclear phosphoprotein 32-related protein O94813 Slit homolog 2 protein. Thought to act as a molecular guidance cue in cellular migration Q7KQL3 ADP-ribosylation...”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...numbers Thermotoga maritima (O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens (P23526). The tree was generated by...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...includes the sequences of the following species (UniProt accession code): Lupinus luteus (Q9SP37), Plasmodium falciparum (P50250), Burkholderia pseudomallei (Q3JY79), Mycobacterium tuberculosis (P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4...”
- Discovery-2: an interactive resource for the rational selection and comparison of putative drug target proteins in malaria
Mpangase, Malaria journal 2013 - “...protein entry could be retrieved by either using the PlasmoDB identifier PFE1050w, its UniProt accession P50250 or the enzyme name, S-adenosyl-L-homocysteine hydrolase. Summary The "Summary" tab (Figure 1 ) provides the user with the protein identifier from PlasmoDB, VectorBase or Ensembl depending on the organism from...”
- The RNA structurome in the asexual blood stages of malaria pathogen plasmodium falciparum
Alvarez, RNA biology 2021 (secret) - Insights into the intracellular localization, protein associations and artemisinin resistance properties of Plasmodium falciparum K13
Gnädig, PLoS pathogens 2020 - “...ALG2 CUL3 ubiquitin ligase adaptor protein, with role in dolichol metabolism 2.32% 6 11 (5,6) PF3D7_0520900 Adenosylhomocysteinase SAHH Methionine metabolism 2.15% 3 6 (3,3) PF3D7_1026800 40S ribosomal protein S2 RPS2 Small ribosomal subunit 1.76% 6 12 (5,7) PF3D7_1444800 Fructose-bisphosphate aldolase FBPA Glycolysis 1.72% 3 6 (3,3)...”
- Transcriptome analysis based detection of Plasmodium falciparum development in Anopheles stephensi mosquitoes
Oakley, Scientific reports 2018 - “...reductase PF3D7_0922900 Day 8 protein DJ-1 PF3D7_0627500 Day 8 Adenosine deaminase PF3D7_1029600 Day 8 Adenosylhomocysteinase PF3D7_0520900 Day 8 ATP synthase F1, alpha subunit PF3D7_0217100 Day 6 Cytochrome c oxidase subunit 2 PF3D7_1430900 Day 8 Dihydrofolate synthase/folylpolyglutamate synthase PF3D7_1324800 Day 2 Fumarate hydratase PF3D7_0927300 Day 6 NADP-specific...”
- Artemisinin resistance without pfkelch13 mutations in Plasmodium falciparum isolates from Cambodia
Mukherjee, Malaria journal 2017 - “...determine copy numbers for pfmdr1, plasmepsin II and the 63kb amplicon genes (PF3D7_0520100, PF3D7_0520500, PF3D7_0520600, PF3D7_0520900 and PF3D7_0521000), real time quantitative PCR was performed on genomic DNA (extracted with QIAmp Blood Mini Kit, Qiagen) as previously described [ 28 ] with the following modifications: Amplification reactions...”
- “...loci: pfmdr1 (PF3D7_0523000) [ 29 ], the 63kb region on chromosome 5 (PF3D7_0520100, PF3D7_0520500, PF3D7_0520600, PF3D7_0520900 and PF3D7_0521000 [ 20 ]) and plasmepsin II (PF3D7_140800) [ 22 ]. For the endogenous controls, - tubulin forward and reverse primers [ 28 ] were used for pfmdr1, PF3D7_0520100...”
- Profiling invasive Plasmodium falciparum merozoites using an integrated omics approach
Kumar, Scientific reports 2017 - “...sets were normalized to five constitutively expressed proteins, which were (PF3D7_0406100 (V ATPase subunit B), PF3D7_0520900 (adenosylhomocysteinase), PF3D7_0624000 (hexokinase), PF3D7_1115600 (peptidyl-prolyl cis-trans isomerase) and PF3D7_1117700 (RAN GTPase)) (Supplemental Fig. S4 ). These five proteins also were identified to have minimal differences in their mRNA expression levels...”
- “...The peak intensity data were further normalized using a set of five house-keeping proteins (PF3D7_0406100, PF3D7_0520900, PF3D7_0624000, PF3D7_1115600 and PF3D7_1117700). The peptide identification was done by searching the MS E data against WT proteome to link identified peptides to their corresponding peak features. The following search...”
- Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 in Plasmodium falciparum
Hollin, BMC genomics 2016 - “...conserved Plasmodium protein, unknown function QDO/A (1) PF3D7_0520800 conserved Plasmodium protein, unknown function TDO/A (1) PF3D7_0520900 S-adenosyl-L-homocysteine hydrolase (SAHH) TDO/A (1) PF3D7_0610100 Pre-mRNA-splicing factor SLU7, putative (SLU7) TDO/A (1) PF3D7_0611800 conserved Plasmodium protein, unknown function TDO/A (1) PF3D7_0613800 transcription factor with AP2 domain(s) (ApiAP2) QDO/A (1)...”
- “...ribosomal protein L2 (RPL2) TDO/A (1) KVIF PF3D7_0520800 conserved Plasmodium protein, unknown function QDO/A (1) PF3D7_0520900 S-adenosyl-L-homocysteine hydrolase (SAHH) TDO/A (1) PF3D7_0525200 conserved Plasmodium protein, unknown function TDO/A (2) KIEF/KDVLF PF3D7_0530000 conserved Plasmodium protein, unknown function TDO/A (1) PF3D7_0532100 early transcribed membrane protein 5 (ETRAMP5) TDO/A...”
- Widespread occurrence of lysine methylation in Plasmodium falciparum proteins at asexual blood stages
Kaur, Scientific reports 2016 - “...ANAIITNLYLDGTLIIENk PF3D7_0517500 UTPglucose-1-phosphate uridylyltransferase, putative C-Term(Methyl) HILIC VQSINSTkHNNNIK PF3D7_0518100 RAP protein, putative K8(Methyl) HILIC nETLEEYWWcVESALTWGDGDDNGPDMIVDDGGDATLLVHk PF3D7_0520900 S-adenosyl-L-homocysteine hydrolase (SAHH) K41(Methyl) HILIC EDkENLNDMYSVNVTNHnDEk PF3D7_0521300 conserved Plasmodium protein, unknown function K3(Trimethyl); C-Term(Methyl) IP QNLNASNSSGQENkQnESDGK PF3D7_0524600 50S ribosomal protein L12, apicoplast, putative K14(Dimethyl) IP VPQGALNNIFSICYTSGTTGYPk PF3D7_0525100 acyl-CoA synthetase (ACS10)...”
- Plasmodium falciparum Resistance to Artemisinin Derivatives and Piperaquine: A Major Challenge for Malaria Elimination in Cambodia
Duru, The American journal of tropical medicine and hygiene 2016 - “...copy number variations (CNVs) for the PF3D7_0520500 and PF3D7_0520900 genes located on chromosome 5 previously identified by Eastman and others.85-87 Spring and...”
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A0A0L8G425 Adenosylhomocysteinase from Octopus bimaculoides
59% identity, 71% coverage
B7PCR2 S-adenosylhomocysteine hydrolase, putative from Ixodes scapularis
47% identity, 93% coverage
XP_011538837 S-adenosylhomocysteine hydrolase-like protein 1 isoform X1 from Homo sapiens
48% identity, 83% coverage
- NRAS Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth.
Cai, Molecular cancer research : MCR 2024 - GeneRIF: NRAS Mutant Dictates AHCYL1-Governed ER Calcium Homeostasis for Melanoma Tumor Growth.
- The role of S-adenosylhomocysteine hydrolase-like 1 in cancer.
Budnik, Biochimica et biophysica acta. Molecular cell research 2024 (PubMed)- GeneRIF: The role of S-adenosylhomocysteine hydrolase-like 1 in cancer.
- Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tau.
Wischhof, The Journal of biological chemistry 2022 - GeneRIF: Unbiased proteomic profiling reveals the IP3R modulator AHCYL1/IRBIT as a novel interactor of microtubule-associated protein tau.
- Adenosylhomocysteinase like 1 interacts with nonstructural 5A and regulates hepatitis C virus propagation.
Lim, Journal of microbiology (Seoul, Korea) 2021 (PubMed)- GeneRIF: Adenosylhomocysteinase like 1 interacts with nonstructural 5A and regulates hepatitis C virus propagation.
- Both IRBIT and long-IRBIT bind to and coordinately regulate Cl-/HCO3- exchanger AE2 activity through modulating the lysosomal degradation of AE2.
Itoh, Scientific reports 2021 - GeneRIF: Both IRBIT and long-IRBIT bind to and coordinately regulate Cl(-)/HCO3(-) exchanger AE2 activity through modulating the lysosomal degradation of AE2.
- Low IRBIT Levels Are Associated With Chemo-resistance in Gastric Cancer Patients.
Nakazawa, Anticancer research 2019 (PubMed)- GeneRIF: in the group receiving postoperative adjuvant chemotherapy, a significant association was found between IRBIT expression and both overall and disease-free survival. IRBIT may be used as a useful predictive marker for chemotherapy.
- Modulation of Cl- signaling and ion transport by recruitment of kinases and phosphatases mediated by the regulatory protein IRBIT.
Vachel, Science signaling 2018 - GeneRIF: IRBIT controlled five phosphorylation sites in NBCe1-B that determined both the active conformation of the transporter and its regulation by Cl(-)
- Mutations in S-adenosylhomocysteine hydrolase (AHCY) affect its nucleocytoplasmic distribution and capability to interact with S-adenosylhomocysteine hydrolase-like 1 protein.
Grbeša, European journal of cell biology 2017 (PubMed)- GeneRIF: We investigated previously assumed interaction with AHCY-like-1 protein (AHCYL1), a paralog of AHCY. Indeed, significant interaction between both proteins exists. Additionally, silencing AHCYL1 leads to moderate inhibition of nuclear export of endogenous AHCY.
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FGSG_05615 adenosylhomocysteinase from Fusarium graminearum PH-1
58% identity, 69% coverage
Q6YBX8 adenosylhomocysteinase (EC 3.3.1.1) from Cryptosporidium parvum (see paper)
GI|37725005 S-adenosylhomocysteine hydrolase; EC 3.3.1.1 from Cryptosporidium parvum (see paper)
42% identity, 92% coverage
5jpiA / Q5CPH1 2.15 angstrom crystal structure of s-adenosylhomocysteinase from cryptosporidium parvum in complex with d-eritadenine and NAD
42% identity, 92% coverage
- Ligands: nicotinamide-adenine-dinucleotide; d-eritadenine (5jpiA)
XP_011239384 putative adenosylhomocysteinase 3 isoform X3 from Mus musculus
52% identity, 60% coverage
P50252 adenosylhomocysteinase (EC 3.3.1.1) from Saccharolobus solfataricus (see paper)
41% identity, 94% coverage
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...from Uniprot ( https://www.uniprot.org/ ) using the following accession numbers Thermotoga maritima (O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247),...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...(P9WGV3), Pseudomonas aeruginosa (Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD + binding in the Rossmann fold-based CBD of SAHases. Residues located in the region of loop...”
SSO0755 S-adenosyl-L-homocysteine hydrolase (ahcY) from Sulfolobus solfataricus P2
41% identity, 94% coverage
- Something old, something new, something borrowed; how the thermoacidophilic archaeon Sulfolobus solfataricus responds to oxidative stress
Maaty, PloS one 2009 - “...6.13 55 P9 COG1517 serine hydroxymethyltransferase SSO0530 48535 6.22 261,463 P11, P12 COG0112 S-adenosylhomocysteine hydrolase SSO0755 45936 6.3 146, 189 P11, P12 COG0499 Elongation factor 1-alpha * SSO0216 48573 8.93 573,104 P13, P14 COG5256 * Found among the regulated proteins, arrow indicates status of regulation Chemical...”
SiRe_1254 adenosylhomocysteinase from Saccharolobus islandicus REY15A
41% identity, 94% coverage
- PerR functions as a redox-sensing transcription factor regulating metal homeostasis in the thermoacidophilic archaeon Saccharolobus islandicus REY15A
Zhang, Nucleic acids research 2025 - “...), sire_0495 ( duf973 ), sire_0980 , sire_0982 ( duf1641 ), sire_1083 ( tpp ), sire_1254 ( sam1 ), sire_1398 , sire_1880 ( tadC ), sire_2636 ( vit1/ccc1 ) and tbp were chosen for the analysis. Sa. islandicus E233S and perR strains carrying each of the...”
- “...1.02454 3.19876 SiRe_0982 Uncharacterized protein YjgD, DUF1641 family 0.83820 4.29584 SiRe_1083 Pyruvate oxidase 0.81682 4.84284 SiRe_1254 S-adenosylhomocysteine hydrolase 1.22631 3.57772 SiRe_1398 Uncharacterized protein 1.04448 3.12305 SiRe_1880 Pilus assembly protein TadC 0.997167 4.60602 SiRe_2636 Predicted Fe 2+ /Mn 2+ transporter, VIT1/CCC1 family 12.78334 - a The promoters...”
Q4JAZ7 Adenosylhomocysteinase from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
40% identity, 94% coverage
O67240 Adenosylhomocysteinase from Aquifex aeolicus (strain VF5)
40% identity, 94% coverage
AT4G13940 MEE58 (MATERNAL EFFECT EMBRYO ARREST 58); adenosylhomocysteinase from Arabidopsis thaliana
52% identity, 55% coverage
- Metabolic enzymes moonlight as selective autophagy receptors to protect plants against viral-induced cellular damage
Clavel, 2024 - New Insights on the Role of ß-Cyanoalanine Synthase CAS-C1 in Root Hair Elongation through Single-Cell Proteomics
Arenas-Alfonseca, Plants (Basel, Switzerland) 2023 - “...(ACL) family protein AT3G28715 ATPase, V0/A0 complex, subunit C/D AT3G28710 ATPase, V0/A0 complex, subunit C/D AT4G13940 ATSAHH1-HOG1 ATSAHH1__S-adenosyl-L-homocysteine hydrolase AT3G23810 ATSAHH2 ATSAHH2__S-adenosyl-l-homocysteine (SAH) hydrolase 2 AT3G48340 CEP2 CEP2__Cysteine proteinases superfamily protein AT2G42490 CuAO-zeta CuAO-zeta__Copper amine oxidase family protein AT3G53580 diaminopimelate epimerase family protein AT1G13950 ATELF5A ELF5A-1__eukaryotic...”
- Plant homocysteine, a methionine precursor and plant's hallmark of metabolic disorders
Sobieszczuk-Nowicka, Frontiers in plant science 2022 - “...( SbCBL ); Zm00001d045153 ( ZmCBL ); LOC_Os06g07860 ( OsCBL1 ); LOC_Os06g07960 ( OsCBL2 ); AT4G13940 ( AtSAHH1 ); AT3G23810 ( AtSAHH2 ); Glyma.11G254700 ( GmSAHH1 ); Glyma.05G152000 ( GmSAHH2 ); Glyma.08G108800 ( GmSAHH3 ); Potri.001G320500 ( PtSAHH1 ); Potri.017G059400 ( PtSAHH2 ); PGSC0003DMG400004572 ( StSAHH...”
- Microtubule Dynamics Plays a Vital Role in Plant Adaptation and Tolerance to Salt Stress
Chun, International journal of molecular sciences 2021 - “...in A120 cells ( Figure 5 ). However, the expression of other genes, such as AT4G13940 (SAHH1), AT4G20260 (PCAP1), and AT4G10480 (NAC4), in this cluster was decreased in A120 cells. Those of AT2G47470 (PDIL2-1), AT1G21750 (PDIL1-1), AT3G62030 (CYP20-3), and AT5G59880 (ADF3) were similar in A0 and...”
- “...showed a hypersensitive phenotype to salt and drought stresses [ 60 , 61 ]. SAHH1 (AT4G13940), encoding S-adenosyl-L-homocysteine hydrolase 1, is involved in the interaction between cytokinin and DNA methylation. Protein disulfide isomerase-like (PDIL) protein 1-1 (PDIL1-1, AT1G21750), which plays essential roles in ER trafficking, is...”
- Identification and Characterization of Plant-Interacting Targets of Tomato Spotted Wilt Virus Silencing Suppressor
Zhai, Pathogens (Basel, Switzerland) 2021 - “...anhydrase LOC107768773 Solyc02g086820 AT3G01500 Heat shock protein 90 (HSP90) LOC107768797 Solyc12g015880 AT5G56000 Adenosylhomocysteinase LOC107783029 Solyc09g092380 AT4G13940 Chloroplast photosystem II subunit O2 (PSBO2) LOC107766588 Solyc02g065400 AT3G50820 Chloroplast photosystem II subunit P1 (PSBP1) LOC107830202 Solyc07g044860 AT1G06680 MP-interacting protein (MIP) 1.2 LOC107801992 Solyc04g009770 AT3G44110 Heat shock protein 70 (HSP70)...”
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms in Arabidopsis Thaliana
Willems, Frontiers in plant science 2021 - “...Plant UBX domain-containing protein 1 (PUX1) Met22 Ac-ME AT1G71840 WD-40 repeat family protein Met10 Ac-MN AT4G13940 Adenosylhomocysteinase 1 (SAHH) Leu57(TTG)->Met NH 2 -S AT2G39080 NAD(P)-binding Rossmann-fold superfamily protein Met59 Ac-A AT2G43290 Calmodulin-like 5 (CML5) Met47 Ac-ML AT3G47590 Alpha/beta-Hydrolases superfamily protein Met50 Ac-MD AT1G53280 Protein DJ-1 homolog...”
- GSNOR Contributes to Demethylation and Expression of Transposable Elements and Stress-Responsive Genes
Rudolf, Antioxidants (Basel, Switzerland) 2021 - “...from The Arabidopsis Information Resource ( www.arabidopsis.org accessed on 24 December 2019) are as follows: AT4G13940 ( SAHH1 ), AT5G43940 ( GSNOR1 ). RNA-seq and WGBS data were stored in the European Nucleotide Archive (ENA) under the ENA accession number PRJEB43942. 3. Results 3.1. GSNOR and...”
- Molecular signatures of silencing suppression degeneracy from a complex RNA virus
Ambrós, PLoS computational biology 2021 - “...isolate T36) targets an S-adenosylmethionine synthetase (SAM2; locus AT4G01850) and an S-adenosyl-L-homocysteine hydrolase (SAH1; locus AT4G13940), which are also required for methylation [ 36 , 37 ]. Interestingly, SAH1 was also reported as a target of HC-Pro [ 38 ]. Additionally, both viral proteins target four...”
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7r39A / Q4JAZ7 Crystal structure of s-adenosyl-l-homocysteine hydrolase from sulfolobus acidocaldarius in complex with adenosine (see paper)
39% identity, 94% coverage
- Ligands: nicotinamide-adenine-dinucleotide; adenosine (7r39A)
SIHH_METJA / Q58783 S-inosyl-L-homocysteine hydrolase; SIHH; EC 3.13.1.9 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
Q58783 S-inosyl-L-homocysteine hydrolase (EC 3.13.1.9) from Methanocaldococcus jannaschii (see paper)
MJ1388 adenosylhomocysteinase (ahcY) from Methanocaldococcus jannaschii DSM 2661
38% identity, 94% coverage
- function: Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L- methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine. Is specific for SIH and inosine as it is unable to either hydrolyze SAH or synthesize SAH from adenosine and Hcy.
catalytic activity: S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine (RHEA:59828)
cofactor: NAD(+) (Binds 1 NAD(+) per subunit.)
subunit: Exists both as a homotetramer and a homodimer, in a 4:1 ratio. - S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling
Miller, Journal of bacteriology 2015 - “...coli. The MJ1388 gene (UniProt accession number Q58783) was amplified by PCR from genomic DNA using oligonucleotide primers MJ1388-Fwd,...”
- “...(in order from the top down): Q58783, M. jannaschii; C7P826, Methanocaldococcus fervens; O27673, Methanothermobacter thermautotrophicus; Q6LYR8, Methanococcus...”
- S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling
Miller, Journal of bacteriology 2015 - “...this uncommon metabolite. An annotated SAHH encoded by MJ1388, which shares greater than 45% sequence identity with other characterized SAHHs (Fig. 3), was...”
- “...Cloning, overexpression, and purification of the M. jannaschii MJ1388 gene product in E. coli. The MJ1388 gene (UniProt accession number Q58783) was amplified...”
P50251 adenosylhomocysteinase (EC 3.3.1.1) from Pyrococcus furiosus (see paper)
8qnoA / P50251 Crystal structure of s-adenosyl-l-homocysteine hydrolase treated at 368 k from pyrococcus furiosus in complex with inosine (see paper)
WP_011011458 adenosylhomocysteinase from Pyrococcus furiosus DSM 3638
38% identity, 94% coverage
C7P826 S-inosyl-L-homocysteine hydrolase from Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86)
38% identity, 94% coverage
TON_1212 S-adenosyl-L-homocysteine hydrolase from Thermococcus onnurineus NA1
38% identity, 94% coverage
Cthe_1200 adenosylhomocysteinase from Clostridium thermocellum ATCC 27405
36% identity, 94% coverage
O27673 S-inosyl-L-homocysteine hydrolase from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
36% identity, 94% coverage
C9RID4 S-inosyl-L-homocysteine hydrolase from Methanocaldococcus vulcanius (strain ATCC 700851 / DSM 12094 / M7)
37% identity, 94% coverage
7o5lA / P74008 Crystal structure of s-adenosyl-l-homocysteine hydrolase from synechocystis sp. Pcc 6803 cocrystallized with adenosine in the presence of rb+ cations (see paper)
38% identity, 94% coverage
- Ligands: nicotinamide-adenine-dinucleotide; rubidium ion; adenosine (7o5lA)
sll1234 S-adenosylhomocysteine hydrolase from Synechocystis sp. PCC 6803
P74008 Adenosylhomocysteinase from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
38% identity, 94% coverage
- Time-course analysis of cyanobacterium transcriptome: detecting oscillatory genes
Layana, PloS one 2011 - “...sll0329 * 6-phosphogluconate dehydrogenase Pentose phosphate pathway 31 F sll1196 * Phosphofructokinase Glycolysis 32 F sll1234 # Adenosylhomocysteinase Amino acids and amines 33 F sll1479 * 6-phosphogluconolactonase Pentose phosphate pathway 34 F slr0301 Phosphoenolpyruvate synthase Pyruvate metabolism 35 F slr0394 # Phosphoglycerate kinase Glycolysis 36 F...”
- Highly expressed and alien genes of the Synechocystis genome
Mrázek, Nucleic acids research 2001 - “...(glgP2) CM 1.36 316 slr1963 ORF 1.36 424 sll1234 S-adenosylhomocysteine hydrolase (ahcY) EM 1.36 358 sll1091 Geranylgeranyl hydrogenase (chlP) BC 1.35 393...”
- Effects of pirfenidone targeting the tumor microenvironment and tumor-stroma interaction as a novel treatment for non-small cell lung cancer.
Fujiwara, Scientific reports 2020 - “...blotting after extraction of membrane proteins was performed using a Cell Surface Protein Isolation Kit (P74008, Takara, Tokyo, Japan) according to the manufacturers protocol. Quantification of immunoblots was performed by Image Lab software as per the manufacturers instructions (Bio-Rad Laboratories, Inc.). Allexperimentswere performed twotimes independently. Data...”
- Identification and characterization of three differentially expressed genes, encoding S-adenosylhomocysteine hydrolase, methionine aminopeptidase, and a histone-like protein, in the toxic dinoflagellate Alexandrium fundyense
Taroncher-Oldenburg, Applied and environmental microbiology 2000 - “...and 62% identity; and a cyanobacterium (Synechocystis sp.; P74008), 63% similarity and 44% identity (Fig. 5A). Methionine aminopeptidase. The 115-bp band B was...”
Q6LYR8 S-inosyl-L-homocysteine hydrolase from Methanococcus maripaludis (strain DSM 14266 / JCM 13030 / NBRC 101832 / S2 / LL)
36% identity, 95% coverage
7r3aA / Q6LYR8 Crystal structure of s-adenosyl-l-homocysteine hydrolase from methanococcus maripaludis in complex with inosine (see paper)
36% identity, 94% coverage
- Ligands: inosine; nicotinamide-adenine-dinucleotide (7r3aA)
O28279 Adenosylhomocysteinase from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)
38% identity, 92% coverage
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...https://www.uniprot.org/ ) using the following accession numbers Thermotoga maritima (O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0), Mus musculus (P50247), and Homo sapiens...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...(Q9I685), Cytophaga hutchinsonii (A0A6N4SNR7), Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD + binding in the Rossmann fold-based CBD of SAHases. Residues located in the region of loop 1 and the...”
- S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling
Miller, Journal of bacteriology 2015 - “...Methanococcus maripaluids; C9RID4, Methanocaldococcus vulcanius; O28279, Archaeoglobus fulgidus; Q4JAZ7, Sulfolobus acidocaldarius; P50251, Pyrococcus furiosus;...”
D7E8N1 S-inosyl-L-homocysteine hydrolase from Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303)
36% identity, 94% coverage
cbdbA477 adenosylhomocysteinase from Dehalococcoides mccartyi CBDB1
35% identity, 96% coverage
- Identification and characterization of a bacterial core methionine synthase
Deobald, Scientific reports 2020 - “...enzymes of the Dehalococcoides methionine metabolism are annotated 34 36 . The loci cbdbA476 and cbdbA477 in the genome of strain CBDB1 are annotated as SAM synthetase (EC 2.5.1.6) and S -adenosyl-L-homocysteinase (EC 3.3.1.1), respectively. Since methionine biosynthesis and SAM metabolism are biochemically closely linked, we...”
- “...the genome of strain CBDB1 by inspecting the direct neighborhood of the loci cbdbA476 and cbdbA477. Locus cbdbA481 located in the same operon encodes a 343 amino acid polypeptide (here referred to as core-MetE CBDB ) with a calculated molecular mass of 38kDa that has sequence...”
O51933 adenosylhomocysteinase (EC 3.3.1.1) from Thermotoga maritima (see 5 papers)
TM0172 adenosylhomocysteinase from Thermotoga maritima MSB8
37% identity, 90% coverage
- Structure-based activity prediction for an enzyme of unknown function
Hermann, Nature 2007 - “...we measured the activity of S-adenosyl homocysteinase (Tm0172), which hydrolyses SAH to homocysteine and adenosine, using SIH as a potential substrate....”
- “...1 and Supplementary Information). This is consistent with Tm0172 and Tm0936 participating in a degradation pathway, though it does not confirm it. We cannot...”
- Recombination in Thermotoga: implications for species concepts and biogeography
Nesbø, Genetics 2006 - “...from the 15,787 bp shared by all the fosmids spanning TM0172 to 23S rRNA (yellow rectangle in Figure 1) is shown in Figure 2B and differs from that found...”
- Functional and Pathological Roles of AHCY
Vizán, Frontiers in cell and developmental biology 2021 - “...sequences were obtained from Uniprot ( https://www.uniprot.org/ ) using the following accession numbers Thermotoga maritima (O51933), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), Pseudomonas aeruginosa (Q9I685), Plasmodium falciparum (P50250), Mycobacterium tuberculosis (P9WGV3), Lupinus luteus (Q9SP37), Burkholderia pseudomallei (Q3JY79), Cytophaga hutchinsonii (A0A6N4SNR7), Saccharomyces cerevisiae (A0A6A5PY71) Trypanosoma brucei (Q383X0),...”
- S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains
Brzezinski, Biomolecules 2020 - “...Trypanosoma brucei (Q383X0), Homo sapiens (P23526), Saccharolobus solfataricus (P50252), Archaeoglobus fulgidus (O28279), and Thermotoga maritima (O51933). Figure 4 The canonical mode of NAD + binding in the Rossmann fold-based CBD of SAHases. Residues located in the region of loop 1 and the N-terminal end of 1...”
3x2fA / O51933 A thermophilic s-adenosylhomocysteine hydrolase (see paper)
37% identity, 90% coverage
- Ligand: 1,4-dihydronicotinamide adenine dinucleotide (3x2fA)
tll2390 S-adenosyl-L-homocysteine hydrolase from Thermosynechococcus elongatus BP-1
38% identity, 94% coverage
MA1275 adenosylhomocysteinase from Methanosarcina acetivorans C2A
39% identity, 90% coverage
D7E8L4 S-inosyl-L-homocysteine hydrolase from Methanohalobium evestigatum (strain ATCC BAA-1072 / DSM 3721 / NBRC 107634 / OCM 161 / Z-7303)
35% identity, 94% coverage
I7GBN2 Macaca fascicularis brain cDNA clone: QflA-17121, similar to human S-adenosylhomocysteine hydrolase-like 1 (AHCYL1), mRNA, RefSeq: NM_006621.3 from Macaca fascicularis
45% identity, 63% coverage
HTSR_0160 adenosylhomocysteinase from Halodesulfurarchaeum formicicum
33% identity, 96% coverage
- Discovery of anaerobic lithoheterotrophic haloarchaea, ubiquitous in hypersaline habitats
Sorokin, The ISME journal 2017 - “...(10) methionine synthase (HTSR_1805); (11) S-adenosylmethionine hydroxide adenosyltransferase (HTSR_1447); (12) S-adenosylmethionine-dependent methyltransferase (HTSR_1450); (13) adenosylhomocysteinase (HTSR_0160); (14) D-3-phosphoglycerate dehydrogenase (HTSR_0539), (15) phosphoserine phosphatase (HTSR_1388). The data for the 150 most abundant proteins from proteomic analysis are outlined in the small nested box. The proteins are sorted...”
A0A7J8X274 adenosylhomocysteinase from Gossypium aridum
45% identity, 55% coverage
- Evolutionary Aspects of Selenium Binding Protein (SBP)
Dervisi, Journal of molecular evolution 2023 - “...potentially involved in cysteine and methionine metabolism and sulfur metabolism https://string-db.org/network/210143.A0A1R3G161 ), two Gossypium Adenosylhomocysteinases (A0A7J8X274, A0A7J8LSY9), a DYW_deaminase domain-containing protein (D7MH79) and a very complex Unconventional myosin-Ie (A0A3N0XSR5, http://pfam.xfam.org/protein/A0A3N0XSR5 ). Adenosylhomocysteinase is a hydrolase (EC: 3.3.1.1) participating in cysteine and methionine metabolism and thus playing...”
Q9HN50 Adenosylhomocysteinase from Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
33% identity, 92% coverage
K9KA63 Adenosylhomocysteinase-like protein (Fragment) from Equus caballus
52% identity, 20% coverage
- Heat Shock Alters the Proteomic Profile of Equine Mesenchymal Stem Cells.
Abd-El-Aziz, International journal of molecular sciences 2022 - “...the heat-stressed osteoblasts, two of the genes coding for these proteins were identified by homology (K9KA63 and F6SUF8), one of which was a fragment, and, therefore, were not well characterized equine proteins. A newer characterization of these proteins could lead to the identification of other mechanisms...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 798,070 different protein sequences to 1,261,478 scientific articles. Searches against EuropePMC were last performed on May 12 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory