PaperBLAST
PaperBLAST Hits for tr|Q9I624|Q9I624_PSEAE biotin carboxylase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA0494 PE=4 SV=1 (458 a.a., MHKRLNKLLV...)
Show query sequence
>tr|Q9I624|Q9I624_PSEAE biotin carboxylase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA0494 PE=4 SV=1
MHKRLNKLLVANRGEIAVRIIRAAQALGIPTVAVCSEADRDSLAARLADEVRLIGPARAD
RSYLDIEAILMAARETGADSIHPGYGFLSENPAFAEAVQAAGLVFVGPEAQTIRRMGDKA
EARRTAMAAGVPVVPGSPGELGDLDAALACAEEVGYPLLVKASAGGGGRGIRIAHDADEL
RREFPIAQREAQAAFGSPAVYLERFIRQARHIEVQILGDGERAVHLFERECSLQRRRQKV
FEEAPSAALDGAQRQALCESAVRLAQSLGYRGAGTLEYLFDAHSGEFFFIEMNTRIQVEH
PVSEMVTGIDLVQAMLRIAAGEALPWRQEDIRLNGAALEMRINAEDPARNFFPSPGTVER
LEWPQGEGVRVDSHLYPGYRVPPYYDSLLAKLIVHGRDRDEAFARARQALERTVLAGMAT
TLPLHRWLLADARVQAAQFDTATLETWLAERTAAAQEV
Running BLASTp...
Found 250 similar proteins in the literature:
PA0494 acetyl-CoA carboxylase from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
- ECF Sigma Factor HxuI Is Critical for In Vivo Fitness of Pseudomonas aeruginosa during Infection
Cai, Microbiology spectrum 2022 - “...Dnr box 3.77 4.13E-14 PA5275 cyaY Frataxin-like protein; iron-sulfur cluster assembly protein 2.82 1.26E-25 Metabolism PA0494 Probable acyl-CoA carboxylase (ACCase) subunit 2.44 0.0000198 PA0495 Allophanate hydrolase 2.72 1.72E-08 PA0496 Allophanate hydrolase 3.08 8E-24 PA1522 xdhC Xanthine dehydrogenase accessory protein 2.07 3.09E-15 PA2003 bdhA 3-Hydroxybutyrate dehydrogenase 2.07...”
- Introducing differential RNA-seq mapping to track the early infection phase for Pseudomonas phage ɸKZ
Wicke, RNA biology 2021 - “...dioxygenase 55.1 176.6 2.09 1.15E-18 unknown cluster PA0492 conserved hypothetical protein 19 122.4 3.10 7.32E-21 PA0494 probable acyl-CoA carboxylase 11 51 2.64 5.42E-08 PA0495 hypothetical protein 15 72 2.74 1.51E-10 PA0125 parD ParD antitoxin 49.5 181 2.25 5.99E-22 PA3531 bfrB Bacterioferritin 661.5 2766.5 2.46 1.20E-86 The...”
- The Essential Role of Hypermutation in Rapid Adaptation to Antibiotic Stress
Mehta, Antimicrobial agents and chemotherapy 2019 - “...G227D, W239* Frameshifts, W33* 1.06e4 1.46e4 1.96e4 gltS PA4900 PA0494 A194V, G351S, L126L L321P, P339P, Q4* A44V, P56L, Y201Y 4.86e4 6.45e4 6.96e4 Gene name by...”
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa
Fléchard, Scientific reports 2018 - “...accC (PA4848). Another putative biotin carboxylase homolog sharing 55% identity with AccC is encoded by PA0494, a gene lying in the PA0493-PA0496 operon, whose transcript level is also strongly decreased (>6) in PAOSX (Supplementary Table S3). Moreover, the product of PA0493 shares 40% identity with AccB....”
- Increased ParB level affects expression of stress response, adaptation and virulence operons and potentiates repression of promoters adjacent to the high affinity binding sites parS3 and parS4 in Pseudomonas aeruginosa
Kawalek, PloS one 2017 - “...parS6 PA0492 -6,7 -2,8 6 conserved hypothetical protein PA0493 -4,3 -2,4 6 probable biotin-requiring enzyme PA0494 -3,0 -2,2 6 probable acyl-CoA carboxylase subunit PA0495 -3,6 -2,1 6 hypothetical protein The biofilm formation was checked in the cultures of PAO1161 (pKGB9 araBAD p- parB ) in comparison...”
- “...other parS sequences ( S5 Table ). However, the expression of PA0492 , PA0493 and PA0494 as assayed by RT-qPCR was not significantly changed in parB null or parS null cells in comparison with the WT strain ( Fig 6B ), indicating that at its native...”
- ChIP-Seq and RNA-Seq reveal an AmrZ-mediated mechanism for cyclic di-GMP synthesis and biofilm development by Pseudomonas aeruginosa
Jones, PLoS pathogens 2014 - “...transcriptional regulator RhlR PA1664 2.86 5.77 OrfX (secreted by Type VI) PA4033 2.75 5.32 MucE PA0494 2.73 8.98 acetyl-CoA carboxylase biotin carboxylase subunit PA3353 2.73 4.04 predicted c-di-GMP binding protein PA0472 2.61 3.51 RNA polymerase sigma factor FiuI, sigma 24 homolog PA1088 2.56 4.94 Predicted magnesium...”
- The Pseudomonas aeruginosa pfpI gene plays an antimutator role and provides general stress protection
Rodríguez-Rojas, Journal of bacteriology 2009 - “...of product Increase/decrease of transcription (fold) SD PA0494 PA0496 PA0717 PA0718 PA0719 PA0720 PA0721 PA0722 PA0723 PA0725 PA0726 PA0758 PA0830 PA2122...”
- Effect of anaerobiosis and nitrate on gene expression in Pseudomonas aeruginosa
Filiatrault, Infection and immunity 2005 - “...PA0176 PA0177 PA0178 PA0179 PA0200 PA0386 PA0447 PA0459 PA0494 PA0509 PA0510 PA0512 PA0513 PA0514 PA0516 PA0518 PA0519 PA0521 PA0526 PA0586 PA0587 PA0614 PA0623...”
WP_208227955 acetyl-CoA carboxylase biotin carboxylase subunit from Brenneria izadpanahii
71% identity, 99% coverage
JV35_06685 acetyl-CoA carboxylase biotin carboxylase subunit from Pectobacterium betavasculorum
70% identity, 98% coverage
BLJAPNOD_04937 acetyl-CoA carboxylase biotin carboxylase subunit from Ensifer sp. M14
61% identity, 98% coverage
BCAL0660 biotin carboxylase from Burkholderia cenocepacia J2315
65% identity, 92% coverage
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction
Fang, BMC systems biology 2011 - “...gene essentiality analysis, the genome-scale metabolic model of B. cenocepacia was further refined. For example, BCAL0660 and BCAL3421, which were homologous genes encoding protein AccC according to their annotation, were originally both included in the model with the gene-protein-reaction (GPR) relationship of "BCAL0660 or BCAL3421". Through...”
- “...BCAL3421 shows greater than 2-fold expression for J2315 under CF conditions versus soil conditions, yet BCAL0660 shows an opposite result [ 75 ]. Taken together, BCAL0660 was excluded from model i KF1028. Further studies are necessary to validate the function of BCAL0660. Identification of essential enzymes...”
ACIAD1736 biotin carboxylase (A subunit of acetyl-CoA carboxylase) from Acinetobacter sp. ADP1
53% identity, 97% coverage
BAB1_0924 Acetyl-CoA carboxylase, biotin carboxylase:Carbamoyl-phosphate synthase L chain, ATP-binding:Carbamoyl-phosphate synthetase l... from Brucella melitensis biovar Abortus 2308
Q57DK3 Biotin carboxylase from Brucella abortus biovar 1 (strain 9-941)
54% identity, 98% coverage
PP0558, PP_0558 acetyl-CoA carboxylase, biotin carboxylase from Pseudomonas putida KT2440
55% identity, 98% coverage
PFLCHA0_RS03400 acetyl-CoA carboxylase biotin carboxylase subunit from Pseudomonas protegens CHA0
55% identity, 98% coverage
- Experimental-Evolution-Driven Identification of Arabidopsis Rhizosphere Competence Genes in Pseudomonas protegens
Li, mBio 2021 - “...intergenic mutations Nonsynonymous Synonymous Deletions PFLCHA0_RS02080 hutI Imidazolonepropionase F 4 1 0 1 0 0 PFLCHA0_RS03400 accC Biotin carboxylase, acetyl-CoA carboxylase subunit I 2 1 1 0 0 0 PFLCHA0_RS05510 nudL Coenzyme A pyrophosphatase/nudix hydrolase NudL L 4 1 0 1 0 0 PFLCHA0_RS06125 rpoS RNA...”
- “...galE c.94G>A p.Val32Met V32M 2/6 5/6 6/6 2 PFLCHA0_RS30120 tssM c.1336C>T p.Leu446Leu 1336C>T 1/6 2 PFLCHA0_RS03400 accC c.1237G>A p.Glu413Lys E413K 4/6 6/6 2 PFLCHA0_RS13000 yvaQ2 c.-9G>T NA -9G>T 1/6 2 PFLCHA0_RS17965 gacA c.160G>T p.As54Tyr D54Y 2/6 5.5% 4.5% 15.5% x 2 PFLCHA0_RS17965 gacA c.289G>A p.Gly97Ser G97S...”
NGO0044 acetyl-CoA carboxylase, biotin carboxylase from Neisseria gonorrhoeae FA 1090
53% identity, 98% coverage
- Activation of Exogenous Fatty Acids to Acyl-Acyl Carrier Protein Cannot Bypass FabI Inhibition in Neisseria
Yao, The Journal of biological chemistry 2016 - “...carboxylase complex (accABCD, NGO0821, NGO0045, NGO0044, NGO0249), malonyl-CoA:ACP transacylase (fabD, NGO2166), 3-oxoacyl-ACP synthase III (fabH, NGO2168),...”
- Identification of a conserved protein involved in anaerobic unsaturated fatty acid synthesis in Neiserria gonorrhoeae: implications for facultative and obligate anaerobes that lack FabA
Isabella, Molecular microbiology 2011 - “...Carboxylase accA Carboxyl-transferase subunit NGO0821 accB Biotin carboxyl carrier protein (BCCP) NGO0045 accC Biotin carboxylase NGO0044 accD Carboxyl-transferase subunit NGO0249 Saturated Fatty Acid Synthesis acpP apo-ACP NGO1351 fabD Malonyl-CoA:ACP transacylase NGO2166 fabF 3-Ketoacyl-ACP synthase II NGO1763 fabG 3-Ketoacyl-ACP reductase NGO2163 fabH 3-Ketoacyl-ACP synthase III NGO2168 fabI...”
A9HEX0 Biotin carboxylase from Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / CCUG 37298 / CIP 103539 / LMG 7603 / PAl5)
54% identity, 98% coverage
- Arabidopsis thaliana exudates induce growth and proteomic changes in Gluconacetobacter diazotrophicus
Dos, PeerJ 2020 - “...insertional mutants of G. diazotrophicus PAl5, defective in production of the proteins EttA (A9H4G2), AccC (A9HEX0), and Zwf (A9H326) were obtained from the G. diazotrophicus PAl5 mutant library of the Laboratrio de BiotecnologiaUENF ( Intorne et al., 2009 ). Such a mutant library was obtained by...”
- “...4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) ispG 6.00 1,571.93 3.00 A9GZU8 Conserved protein GDI0061 7.00 1,962.62 3.04 A9HEX0 acetyl-CoA carboxylase biotin carboxylase subunit accC 13.00 4,423.06 3.52 A9HS02 Elongation factor G fusA 31.00 10,183.21 3.93 A9H932 TonB-dependent receptor GDI0667 14.00 2,074.37 4.10 ILVC Ketol-acid reductoisomerase (NADP(+)) ilvC 5.00...”
BLJAPNOD_03269 acetyl-CoA carboxylase biotin carboxylase subunit from Ensifer sp. M14
53% identity, 97% coverage
PA4848 biotin carboxylase from Pseudomonas aeruginosa PAO1
PA14_64110 biotin carboxylase from Pseudomonas aeruginosa UCBPP-PA14
56% identity, 97% coverage
- The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa
Fléchard, Scientific reports 2018 - “...the carboxylation of the product of accB (PA4847) by a biotin carboxylase encoded by accC (PA4848). Another putative biotin carboxylase homolog sharing 55% identity with AccC is encoded by PA0494, a gene lying in the PA0493-PA0496 operon, whose transcript level is also strongly decreased (>6) in...”
- Protein complex formation during denitrification by Pseudomonas aeruginosa
Borrero-de, Microbial biotechnology 2017 - “...of pyruvate dehydrogenase (PA5015), pyruvate kinase (PA4329), phosphopyruvate hydratase (PA3035), acetylCoA carboxylase (PA3112, PA3639 and PA4848) and phosphoenolpyruvate synthase (PA1770) (Ornston, 1971 ; Meylan etal ., 2017 ) (Fig. 2 B). The denitrification supercomplex as a platform for corresponding transport and maturation factors The respirasome included...”
- Protein Network of the Pseudomonas aeruginosa Denitrification Apparatus
Borrero-de, Journal of bacteriology 2016 - “...hydratase (PA3035), acetylCoA carboxylase (PA3112, PA3639, and PA4848), and phosphoenolpyruvate synthase (PA1770) were also found to be bound. The...”
- Pseudomonas aeruginosa ceftolozane-tazobactam resistance development requires multiple mutations leading to overexpression and structural modification of AmpC
Cabot, Antimicrobial agents and chemotherapy 2014 - “...PA4673.1 Intergenic PA4745 PA4771 PA4783 PA4819 Intergenic PA4840 PA4846 PA4848 PA4856 gcpE acoR opmD pilB yfiT pilE nusA lldD yedA a 7, P1.2, TOL-TAZr 7,...”
- A systems-level approach for investigating Pseudomonas aeruginosa biofilm formation
Xu, PloS one 2013 - “...prs ), PA4729( panB ),PA4730( panC ),PA4749( glmM ),PA4750( folP ), PA4759( dapB ),PA4847( accB ),PA4848( accC ),PA4854( purH ), PA4920( nadE ),PA4938( purA ),PA4988( waaA ),PA4996( rfaE ), PA5009( waaP ),PA5010( waaG ),PA5011( waaC ),PA5012( waaF )PA5034( hemE ),PA5162( rmlD ),PA5163( rmlA ),PA5175( cysQ ),...”
- A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...of cofactors, prosthetic groups and carriers, cell wall/ Lipopolysaccharide/ capsule UppS (PA3652) Biotin biosynthesis AccC (PA4848) Branched chain amino acid biosynthesis IlvC (PA4694), IlvD (PA0353) Cell envelope biosynthesis GlmU (PA5552), GlmS (PA5549), MurI (PA4662), MraY (PA4415), MurE (PA4417), MurC (PA4411), MurB (PA2977) DdlB (PA4410), DdlA (PA4201),...”
- “...RmlB (PA5161) Citrate acid cycle SdhC (PA1581), SdhD (PA1582) Coenzyme A biosynthesis CoaE (PA4529), Dfp (PA4848) PanE (PA4397) PanB (PA4729) PanC (PA4730) Cysteine metabolism CysC (PA1393) CysH (PA1756) Fatty acid biosynthesis FabB (PA1609) Folate metabolism FolB (PA0582), FolP (PA4750) FolK (PA4728) PhoA (PA3296) Fructose and mannose...”
- Transcriptional and proteomic responses of Pseudomonas aeruginosa PAO1 to spaceflight conditions involve Hfq regulation and reveal a role for oxygen
Crabbé, Applied and environmental microbiology 2011 - “...PA4633 PA4671 PA4692 PA4702 PA4739 PA4740 PA4743 PA4847 PA4848 PA4880 PA4935 PA4944 PA5049 PA5054 PA5067 PA5069 PA5078 PA5117 PA5128 PA5276 PA5316 PA5355 PA5460...”
- Molecular mechanisms of chlorhexidine tolerance in Burkholderia cenocepacia biofilms
Coenye, Antimicrobial agents and chemotherapy 2011 - “...involved in biotin metabolism (PA4847 [homolog, BCAL3420] and PA4848 [BCAL3421]) and PA5557 and PA5558, encoding - and -subunits of the F0F1 ATP synthase...”
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- Identification of the alternative sigma factor SigX regulon and its implications for Pseudomonas aeruginosa pathogenicity
Blanka, Journal of bacteriology 2014 - “...PA14_42350 PA14_42380 PA14_42400 PA14_42430 PA14_42440 PA14_43690 PA14_49160 PA14_64110 PA14_68360 Gene name PA14 sigX::Gmr vs WT PA14b a SigX Regulon in...”
NMB1861 acetyl-CoA carboxylase, biotin carboxylase from Neisseria meningitidis MC58
52% identity, 98% coverage
2vqdA / P37798 Crystal structure of biotin carboxylase from pseudomonas aeruginosa complexed with ampcp (see paper)
56% identity, 97% coverage
- Ligands: phosphomethylphosphonic acid adenosyl ester; magnesium ion (2vqdA)
RHECIAT_CH0001955 acetyl-CoA carboxylase, biotin carboxylase protein from Rhizobium etli CIAT 652
52% identity, 98% coverage
NMA0596 putative acetyl-CoA carboxylase biotin carboxylase component from Neisseria meningitidis Z2491
52% identity, 98% coverage
SACOL1571 acetyl-CoA carboxylase, biotin carboxylase from Staphylococcus aureus subsp. aureus COL
SA1357 acetyl-CoA carboxylase from Staphylococcus aureus subsp. aureus N315
NWMN_1431 acetyl-CoA carboxylase biotin carboxylase subunit from Staphylococcus aureus subsp. aureus str. Newman
B7H15_08395, EKM74_RS01000 acetyl-CoA carboxylase biotin carboxylase subunit from Staphylococcus aureus
51% identity, 96% coverage
- Complete and SOS-mediated response of Staphylococcus aureus to the antibiotic ciprofloxacin
Cirz, Journal of bacteriology 2007 - “...SACOL0987 SACOL0988 SACOL1243 SACOL1244 SACOL1245 SACOL1571 SACOL1572 SACOL2079 Toxin production/resistance and pathogenesis Down-regulated ORFs SACOL1173...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...sa_c1344s1117_a_at * 4.1 2.5 2.5 SA1266 putative DNA processing protein sa_c1643s1381_at * 3.4 2.5 2.5 SA1357 thermonuclease sa_c3053s2604_a_at * 2.4 2.5 30 SA1732 replication initiation and membrane attachment protein Signal transduction sa_c1996s1713_a_at 2.9 2.5 15 arlS SA1450 sensor histidine kinase sa_c6155s5336_a_at * 4.4 2.5 2.5 lytS...”
- “...protein DprA sa_c1522s1300_a_at 3.7 2.5 2.5 SA1326 putative GTP-binding protein sa_c1643s1381_at * 6.3 2.5 ND SA1357 thermonuclease sa_c2640s2213_a_at 11.4 2.5 2.5 SA1615 ATP dependent DNA helicase sa_c2726s2297_a_at 3.6 2.5 2.5 SA1643 DNA polymerase III sa_c2861s2424_a_at 4.0 2.5 2.5 SA1682 recombination factor protein RarA sa_c2977s2534_a_at 2.3 2.5...”
- Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain
Gill, Journal of bacteriology 2005 - “...SA2209 hlgB MW2344 hlgB SA2160 SA0762d SA2194 hysA SA1357 nuc SA0860 SA1264 lytN SA1281 SA0833 clpP SAV2170 SAV0919 SAV2202 SAV1324 SAV0815 SAV1247 SAV1262...”
- Comparative genomics of Staphylococcus aureus musculoskeletal isolates
Cassat, Journal of bacteriology 2005 - “...SA0209 SA0317 SA0712 SA0857 SA0860 SA1028 SA1056 SA1057 SA1357 SA1864 SA1865 SA1866 SA1867 SA1868 SA1869 SA1970 SA2194 SA2549 SA2659 SA2694 SA2003 JAMS1 UAMS601...”
- (p)ppGpp/GTP and Malonyl-CoA Modulate Staphylococcus aureus Adaptation to FASII Antibiotics and Provide a Basis for Synergistic Bi-Therapy
Pathania, mBio 2021 - “...the in vivo promoter fusion P accBC -lacZ to measure expression of accBC (NWMN_1432 and NWMN_1431), which encode subunits of acetyl-CoA carboxylase (ACC) required for malonyl-CoA synthesis ( Table S2 ). Stringent response induction by mupirocin led to decreases in malonyl-CoA pools (6-fold) and in P...”
- Staphylococcus aureus Stress Response to Bicarbonate Depletion
Liberini, International journal of molecular sciences 2024 - “...fatty acid binding protein 2 1 1 Carboxylating Enzymes B7H15_09750 PckA, PEP carboxylase 5 100 B7H15_08395 AccC, acetyl-coA carboxylase 1.2 2 () indicates a downregulation of gene expression in the mutant compared to the parent. nd, not determined....”
- Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus
Peng, International journal of molecular sciences 2022 - “...; alcohol dehydrogenase DNA replication EKM74_RS04795 85.2 44.94 39.85 1.91 1.09611 single-stranded DNA-binding protein Ribosome EKM74_RS01000 77.06 19.54 474.46 30.77 2.622215 accC ; acetyl-CoA carboxylase biotin carboxylase subunit EKM74_RS01125 36.7 12.69 642.43 184.29 4.129847 rpmG ; 50S ribosomal protein L33 EKM74_RS01310 21.83 0.96 168.95 53.18 2.951911...”
Q54755 biotin carboxylase (EC 6.3.4.14) from Synechococcus sp. (see paper)
53% identity, 98% coverage
P49787 Biotin carboxylase 1 from Bacillus subtilis (strain 168)
BSU24340 acetyl-CoA carboxylase biotin carboxylase subunit from Bacillus subtilis subsp. subtilis str. 168
51% identity, 97% coverage
2vpqB / A0A0H3JRR2 Crystal structure of biotin carboxylase from s. Aureus complexed with amppnp (see paper)
51% identity, 96% coverage
- Ligands: phosphoaminophosphonic acid-adenylate ester; magnesium ion (2vpqB)
Smed_0952 acetyl-CoA carboxylase biotin carboxylase subunit from Sinorhizobium medicae WSM419
53% identity, 98% coverage
SMc01345 acetyl-CoA carboxylase biotin carboxylase subunit from Sinorhizobium meliloti 1021
Q92QK1 Biotin carboxylase from Rhizobium meliloti (strain 1021)
53% identity, 98% coverage
AbA118F_2504 acetyl-CoA carboxylase biotin carboxylase subunit from Acinetobacter baumannii
53% identity, 98% coverage
OA858_03000 acetyl-CoA carboxylase biotin carboxylase subunit from Pseudanabaena galeata CCNP1313
52% identity, 97% coverage
CBU_1726 acetyl-CoA carboxylase, biotin carboxylase from Coxiella burnetii RSA 493
51% identity, 98% coverage
IUJ47_RS01520 acetyl-CoA carboxylase biotin carboxylase subunit from Enterococcus faecalis
54% identity, 97% coverage
- Antibacterial Components and Modes of the Methanol-Phase Extract from Commelina communis Linn
Liu, Plants (Basel, Switzerland) 2023 - “...IUJ47_RS01530 0.109 Acetyl-CoA carboxylase IUJ47_RS01515 0.158 Acetyl-CoA carboxylase carboxyltransferase subunit beta IUJ47_RS12350 0.183 Acetate kinase IUJ47_RS01520 0.262 Acetyl-CoA carboxylase biotin carboxylase subunit IUJ47_RS01510 0.294 Acetyl-CoA carboxylase carboxyl transferase subunit alpha IUJ47_RS07835 0.325 Bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase IUJ47_RS07670 0.397 Phosphate acetyltransferase IUJ47_RS08070 0.425 Pyruvate phosphate dikinase IUJ47_RS11250...”
GBAA4408 acetyl-CoA carboxylase, biotin carboxylase from Bacillus anthracis str. 'Ames Ancestor'
50% identity, 96% coverage
SSU1599 biotin carboxylase subunit of acetyl-CoA carboxylase from Streptococcus suis P1/7
SSU05_1799 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus suis 05ZYH33
53% identity, 96% coverage
- Precision Genome Engineering in Streptococcus suis Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology
Gussak, ACS synthetic biology 2023 - “...5.67 N/A repD 6.45 N/A tetA 5.71 lipid biosynthesis SSU1597 accA 5.42 SSU1598 accD 5.45 SSU1599 accC 5.81 SSU1600 fabZ 5.99 SSU1601 fabE 6.15 SSU1602 fabF 6.29 SSU1603 fabG 6.65 SSU1604 fabD 6.56 SSU1605 fabK 6.50 SSU1606 acpP 4.67 SSU1607 fabH 4.05 SSU1608 marR 3.78 SSU1609...”
- Proteomic and Metabolomic Analyses Provide Insights into the Mechanism on Arginine Metabolism Regulated by tRNA Modification Enzymes GidA and MnmE of Streptococcus suis
Gao, Frontiers in cellular and infection microbiology 2020 - “...6 19.46 AccD SSU05_1797 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta 0.2778 8 31.25 AccC SSU05_1799 Biotin carboxylase 0.3785 10 28.88 FabZ SSU05_1800 3-hydroxyacyl-[acyl-carrier-protein] dehydratase 0.3905 2 17.14 AccB SSU05_1801 Biotin carboxyl carrier protein of acetyl-CoA carboxylase 0.4501 1 8.16 FabF SSU05_1802 3-oxoacyl-[acyl-carrier-protein] synthase 2 0.2011...”
- MnmE, a Central tRNA-Modifying GTPase, Is Essential for the Growth, Pathogenicity, and Arginine Metabolism of Streptococcus suis Serotype 2
Gao, Frontiers in cellular and infection microbiology 2019 - “...6 19.46 AccD SSU05_1797 Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta 0.2444 8 31.25 AccC SSU05_1799 Biotin carboxylase 0.3680 10 28.88 FabZ SSU05_1800 3-hydroxyacyl-[acyl-carrier-protein] dehydratase 0.3717 2 17.14 FabF SSU05_1802 3-oxoacyl-[acyl-carrier-protein] synthase 2 0.18734 8 26.52 FabG1 SSU05_1803 3-ketoacyl-ACP reductase 0.2132 13 80.33 FabD SSU05_1804 Malonyl...”
- “...acid biosynthesis pathway were significantly down-regulated ( Table S1 ), which included AccA (SSU05_1796), AccC (SSU05_1799), AccD (SSU05_1797), FabZ (SSU05_1800), FabF (SSU05_1802), and FabD (SSU05_1804). Notably, the expression levels of the proteins ArcA (SSU05_0624), ArcB (SSU05_0626), ArcC (SSU05_0627) in the arginine biosynthesis pathway were decreased in...”
V6Z187 Biotin carboxylase from Streptococcus agalactiae LMG 14747
53% identity, 97% coverage
EF2877 acetyl-CoA carboxylase, biotin carboxylase from Enterococcus faecalis V583
54% identity, 97% coverage
- Transcriptomic and functional analysis of NaCl-induced stress in Enterococcus faecalis
Solheim, PloS one 2014 - “...phospholipid metabolism EF2876 0,03 2,58 2,72 Acetyl-CoA carboxylase, biotin carboxylase Fatty acid and phospholipid metabolism EF2877 0,10 2,51 2,34 (3R)-hydroxymyristoyl-(acyl-carrier-protein) dehydratase Fatty acid and phospholipid metabolism EF2878 0,18 2,31 1,97 Acetyl-CoA carboxylase, biotin carboxyl carrier protein Fatty acid and phospholipid metabolism EF2879 0,28 2,24 1,80 3-oxoacyl-(acyl-carrier-protein)...”
GSU2019 acetyl-CoA carboxylase, biotin carboxylase from Geobacter sulfurreducens PCA
49% identity, 97% coverage
- Evolution of electron transfer out of the cell: comparative genomics of six Geobacter genomes
Butler, BMC genomics 2010 - “...0.92 saccharopine dehydrogenase 39997914 GSU2821 0.91 nitrogenase iron protein 39998542 GSU3453 0.91 uroporphyrinogen decarboxylase 39997117 GSU2019 0.91 acetyl-CoA carboxylase 39997929 GSU2837 0.91 preprotein translocase SecY 39995209 GSU0098 0.91 MglB protein 39995271 GSU0160 0.91 dihydrodipicolinate reductase 39995450 GSU0341 0.91 NADH dehydrogenase I, D subunit 39995452 GSU0343 0.91...”
CbuG_0107 biotin carboxylase from Coxiella burnetii CbuG_Q212
51% identity, 98% coverage
Q39WZ8 Biotin carboxylase from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
48% identity, 97% coverage
SAK_0426 acetyl-CoA carboxylase, biotin carboxylase from Streptococcus agalactiae A909
53% identity, 96% coverage
CPE1074 acetyl-CoA carboxylase from Clostridium perfringens str. 13
48% identity, 98% coverage
xccC / Q5P8S2 4-hydroxyacetophenone carboxylase biotin carboxylase subunit from Aromatoleum aromaticum (strain EbN1) (see paper)
50% identity, 98% coverage
SM12261_RS07530 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus mitis NCTC 12261
53% identity, 97% coverage
C1DLJ7 biotin carboxylase (EC 6.3.4.14) from Azotobacter vinelandii (see paper)
55% identity, 97% coverage
FTH_1537 acetyl-CoA carboxylase biotin carboxylase subunit from Francisella tularensis subsp. holarctica OSU18
FTL_1591 Acetyl-CoA carboxylase, biotin carboxylase subunit from Francisella tularensis subsp. holarctica
FTS_1551 acetyl-CoA carboxylase biotin carboxylase subunit from Francisella tularensis subsp. holarctica FSC200
51% identity, 98% coverage
- Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins
Horák, Soft matter 2012 - “...cyt FTH_1503 Conserved hypothetical protein 69.85/10.14 CM TMH FTH_1536 Conserved hypothetical protein 25.35/6.96 ? cyt FTH_1537 Acetyl-CoA carboxylase biotin carboxylase subunit 50.05/6.85 cyt cyt FTH_1551 S49 family SohB endopeptidase 38.07/9.25 CM TMH FTH_1558 Glycosyltransferase, group 2 family protein 36.08/8.69 CM cyt FTH_1599 Conserved hypothetical protein 49.32/4.33...”
- Francisella tularensis: FupA mutation contributes to fluoroquinolone resistance by increasing vesicle secretion and biofilm formation
Siebert, Emerging microbes & infections 2019 - “...synthetase 20,082 0,00553302 2,07539867 1,730,31 FTL_0449 HTH-MerR Superfamily transcription regulator protein 10,91 0,00624757 3,4734326 0,960,46 FTL_1591 Acetyl-CoA carboxylase, biotin carboxylase subunit 73,596 0,00860376 2,01843356 2,380,85 FTL_1899 Glutamine synthetase 6.3.1.2 74,386 0,00862336 3,80186705 1,230,44 FTL_0738 Hypothetical lipoprotein 53,597 0,00923321 3,8760831 2,291,29 FTL_1832 lucA/IucC family siderophore biosynthesis protein...”
- “...proteins playing a role in replication (FTL_1025, FTL_1407, FTL_1842, FTL_1850, and FTL_1931), glycerolipid (FTL_1644), carbohydrate (FTL_1591 and FTL_1899), and cofactor and vitamin (FTL_1274) metabolism. Some of the differentially-expressed proteins identified may play a direct role in the antibiotic-resistance phenotype of the strain. They include membrane transport...”
- Further Characterization of the Capsule-Like Complex (CLC) Produced by Francisella tularensis Subspecies tularensis: Protective Efficacy and Similarity to Outer Membrane Vesicles
Champion, Frontiers in cellular and infection microbiology 2018 - “...Thioredoxin 1 78 12,194 6.60E-08 FTL_1743 T20,P, S,E DNA-directed RNA polymerase, subunit 77 157,289 6.20E-08 FTL_1591 S Acetyl-CoA carboxylase, biotin carboxylase subunit 76 50,019 7.50E-08 FTL_0097 N Hypothetical protein 76 13,785 6.10E-05 FTL_1780 N, C Triosephosphate isomerase 76 27,638 5.80E-08 FTL_0232 S 30S ribosomal protein S12...”
- Francisella tularensis subsp. holarctica Releases Differentially Loaded Outer Membrane Vesicles Under Various Stress Conditions
Klimentova, Frontiers in microbiology 2019 - “...ABC transporter ATP-binding protein (FTS_1893), isocitrate dehydrogenase (FTS_0587) and acetyl-CoA carboxylase, and biotin carboxylase subunit (FTS_1551). On the other hand, proteins from cluster no. 430, which was decreased in all conditions over control, included two FPI proteins (FTS_0099, IglC and FTS_0107, IglG) and a putative glycosyltransferase...”
TTHA1123 acetyl-CoA carboxylase, biotin carboxylase subunit from Thermus thermophilus HB8
57% identity, 93% coverage
- Transcriptional repression mediated by a TetR family protein, PfmR, from Thermus thermophilus HB8
Agari, Journal of bacteriology 2012 - “...TTHA0417 [FabB(F), AcpP, FabG, FabD, and FabH], TTHA1123 (AccC), TTHA1124 (AccB), TTHA1767 (AccA), and TTHA1768 (AccD) (NCBI accession number NC_006461). Since...”
OCAR_5979 acetyl-CoA carboxylase biotin carboxylase subunit from Oligotropha carboxidovorans OM5
53% identity, 97% coverage
Ssal_01798 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus salivarius 57.I
52% identity, 97% coverage
FTT_0473 Acetyl-CoA carboxylase, biotin carboxylase subunit from Francisella tularensis subsp. tularensis SCHU S4
51% identity, 98% coverage
- The francisella tularensis proteome and its recognition by antibodies
Kilmury, Frontiers in microbiology 2010 - “...( 2007 ), Janovska et al. ( 2007a ), Sundaresh et al. ( 2007 ) FTT_0473 Acetyl-CoA carboxylase, biotin carboxylase subunit accC Mouse 2D-Western blot Cytoplasmic Havlasova et al. ( 2005 ) FTT_0479 PerM family Human, presumed type B Proteome microarray Cytoplasmic membrane Sundaresh et al....”
Caur_3421 acetyl-CoA carboxylase, biotin carboxylase from Chloroflexus aurantiacus J-10-fl
WP_012259259 acetyl-CoA carboxylase biotin carboxylase subunit from Chloroflexus aurantiacus
55% identity, 97% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...CO 2 -anaplerotic pathways tme (Caur_1614), pckA (Caur_2331), ppc (Caur_3161, Caur_3888) 3-hydroxypropionate cycle accC (Caur_1378, Caur_3421), accA (Caur_1647), accD (Caur_1648), accB (Caur_3739), mcr (Caur_2614), pcs (Caur_ 0613), pccB (Caur_2034, Caur_3435), mcee (Caur_3037), mut (Caur_1844, Caur_2508, Caur_2509), smtA (Caur_0179), smtB (Caur_0178), sdhBAC (Caur_1880 to Caur_1882), fh (Caur_1443),...”
- “...cycle were later characterized experimentally [ 4 ]: (1) acetyl-CoA carboxylase ( accC (Caur_1378 and Caur_3421, biotin carboxylase), accA (Caur_1647, -subunit), accD (Caur_1648, -subunit) and accB (Caur_3739) [ 79 ], (2) malonyl-CoA reductase ( mcr , Caur_2614) [ 80 ], (3) propionyl-CoA synthase ( pcs ,...”
- Chloroflexus aurantiacus acetyl-CoA carboxylase evolves fused biotin carboxylase and biotin carboxyl carrier protein to complete carboxylation activity
Shen, mBio 2024 - “...first BC isoform (WP_012257262, BC1) contains 596 amino acid residues, while the second BC isoform (WP_012259259, BC2) comprises 455 residues. During the transition from respiratory to phototrophic conditions, a simultaneous increase in the expression of C. aurantiacus BC1, CT, CT, and most enzymes involved in the...”
- “...C. aurantiacus BC1 and BC2. C. aurantiacus possesses two BC isoforms, BC1 (WP_012257262) and BC2 (WP_012259259). BC1 confers a fused BC (Met1-Glu452) and BCCP domain (Ala459-Lys596), which contains a hairpin (Thr473-Gly486) and a BCCP Core (Lys511-Gln587) at the C-terminus. Multiple sequence alignment revealed the presence of...”
CPF_1330 acetyl-CoA carboxylase, biotin carboxylase from Clostridium perfringens ATCC 13124
48% identity, 98% coverage
sll0053 biotin carboxylase from Synechocystis sp. PCC 6803
51% identity, 97% coverage
T303_03115 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus thermophilus ASCC 1275
52% identity, 97% coverage
- Proteomic analysis reveals potential factors associated with enhanced EPS production in Streptococcus thermophilus ASCC 1275
Padmanabhan, Scientific reports 2020 - “...upregulated. Downregulated proteins were those involved in fatty acid biosynthesis (acetyl-CoA carboxylase biotin carboxylase subunit, T303_03115; acetyl-CoA carboxylase subunit alpha, T303_03125), pyruvate metabolism (isopropylmalate synthase, T303_06880) and citrate cycle (isocitrate dehydrogenase, T303_07275). In glucose medium, there was no significant up/down regulation in other carbohydrate metabolism pathways...”
- Transcriptomic Insights Into the Growth Phase- and Sugar-Associated Changes in the Exopolysaccharide Production of a High EPS-Producing Streptococcus thermophilus ASCC 1275
Padmanabhan, Frontiers in microbiology 2018 - “...pathways (T303_00465, T303_04395, T303_04850, T303_05140, T303_06840, T303_07255, T303_07345, T303_08980, T303_09765) and pyruvate metabolism (T303_02435, T303_03105, T303_03115, T303_03120, T303_06180, T303_06185, T303_06190, T303_06195, T303_08485, T303_09120) were highly expressed at 5 h in M17-G but the expression of most of those genes were reduced or downregulated at 10 h....”
- “...trehalose metabolism (T303_00355, T303_00360, T303_00365) and acetyl CoA carboxylase biotin carrier protein and subunits (T303_03105 T303_03115, T303_03120, T303_03125) were significantly upregulated. FIGURE 7 Changes in expression of genes associated with nucleotide sugar synthesis and carbohydrate metabolism. (A) Heat map of DEGs involved in nucleotide sugar synthesis...”
SPCG_RS02200 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus pneumoniae CGSP14
SP_0425 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus pneumoniae TIGR4
53% identity, 97% coverage
- The transcriptomic response of Streptococcus pneumoniae following exposure to cigarette smoke extract
Manna, Scientific reports 2018 - “...subunit alpha AccA 3.64 0.003 SPCG_RS02205 Acetyl-CoA carboxylase carboxyl transferase subunit beta AccD 3.59 0.003 SPCG_RS02200 Acetyl-CoA carboxylase biotin carboxylase subunit AccC 3.34 0.004 SPCG_RS02190 Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit AccB 3.32 0.004 SPCG_RS02195 3-hydroxyacyl-ACP dehydratase FabZ 3.18 0.005 SPCG_RS02185 3-oxoacyl-ACP synthase FabF 3.11...”
- Multi-omic profiling to assess the effect of iron starvation in Streptococcus pneumoniae TIGR4
Jiménez-Munguía, PeerJ 2018 - “...SP_0413 2/2 Downregulated 38547 SP_0416, SP_0417 2/2 Downregulated 38548 SP_0419, SP_0420, SP_0421, SP_0422, SP_0423, SP_0424, SP_0425, SP_0426, SP_0427, SP_0428, SP_0429, SP_0430, SP_0431 13/13 Downregulated 38571 SP_0526, SP_0527 2/3 Downregulated 38610 SP_0701, SP_0702 2/2 Downregulated 38617 SP_0737, SP_0738 2/2 Downregulated 38620 SP_0750, SP_0751, SP_0752, SP_0753 4/4 Downregulated...”
Q3A2P1 biotin carboxylase (EC 6.3.4.14) from Syntrophotalea carbinolica (see paper)
50% identity, 98% coverage
spr0385 Biotin carboxylase (a subunit of acetyl-CoA carboxylase (ACC) from Streptococcus pneumoniae R6
SPD_0388 acetyl-CoA carboxylase, biotin carboxylase from Streptococcus pneumoniae D39
53% identity, 97% coverage
- Phenotypic characterization of Streptococcus pneumoniae biofilm development
Allegrucci, Journal of bacteriology 2006 - “...gauA (spr1300) lysS (spr0626) glyS (spr1328) accC (spr0385) gpdA (spr1902) rpsA (spr0764) prfC (spr0396) rpsB (spr2020) tufA (spr1343) rplJ (spr1212) exoA...”
- Inactivation of Transcriptional Regulator FabT Influences Colony Phase Variation of Streptococcus pneumoniae
Zhang, mBio 2021 - “...Acetyl-CoA carboxylase biotin carboxyl carrier protein 1.79 0.000 SPD_0387 fabZ 3-Hydroxyacyl-ACP dehydratase FabZ 1.63 0.000 SPD_0388 accC Acetyl-CoA carboxylase biotin carboxylase subunit 2.00 0.000 SPD_0389 accD Acetyl-CoA carboxylase carboxyltransferase subunit beta 2.04 0.000 SPD_0390 accA Acetyl-CoA carboxylase carboxyl transferase subunit alpha 2.13 0.000 SPD_0391 briC Biofilm-regulating...”
- Cadmium stress dictates central carbon flux and alters membrane composition in Streptococcus pneumoniae
Neville, Communications biology 2020 - “...SPD_0386 accB Acetyl-CoA carboxylase, biotin carboxyl carrier protein 1.99 SPD_0387 fabZ 3-hydroxyacyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59) 1.92 SPD_0388 accC Acetyl-CoA carboxylase, biotin carboxylase (EC:6.4.1.2 6.3.4.14) 1.86 SPD_0389 accD Acetyl-CoA carboxylase carboxyl transferase subunit (EC:6.4.1.2 2.1.3.15) 1.79 SPD_0390 accA Acetyl-CoA carboxylase carboxyl transferase subunit (EC:6.4.1.2 2.1.3.15) 1.81 SPD_0684 bioY...”
- Pneumococcal galactose catabolism is controlled by multiple regulators acting on pyruvate formate lyase
Al-Bayati, Scientific reports 2017 - “...SPD_0385 3-oxoacyl-(acyl carrier protein) synthase 5.2 SPD_0386 Acetyl-CoA carboxylase 4.3 SPD_0387 (3R)-hydroxymyristoyl ACP dehydratase 4.6 SPD_0388 Acetyl-CoA carboxylase 6.3 SPD_0389 Acetyl-CoA carboxylase beta subunit 5.4 SPD_0390 Acetyl-CoA carboxylase alpha subunit 5.6 SPD_0407 Hypothetical protein 2 SPD_0420 Formate acetyltransferase 2.9 SPD_0471 Pseudo 2 SPD_0511 5,10-methylenetetrahydrofolate reductase 2.4...”
- The Small Molecule DAM Inhibitor, Pyrimidinedione, Disrupts Streptococcus pneumoniae Biofilm Growth In Vitro
Yadav, PloS one 2015 - “...(accB) acetyl-CoA carboxylase, biotin carboxyl carrier acetyl-CoA carboxylase activity fatty acid biosynthetic process -1.5 (0.001) SPD_0388 (accC) acetyl-CoA carboxylase, biotin carboxylase acetyl-CoA carboxylase activity/biotin carboxylase activity -1.9 (0.05) SPD_0389 (accD) acetyl-CoA carboxylase, carboxyl transferase, acetyl-CoA carboxylase activity/transferase activity fatty acid biosynthetic process -1.7 (0.05) SPD_0390 (accA)...”
SYNPCC7002_A2127 acetyl-CoA carboxylase, biotin carboxylase from Synechococcus sp. PCC 7002
50% identity, 97% coverage
- Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis
Ruffing, Microbial cell factories 2016 - “...(2.42E03) Constitutive (0.0533) 0.256 Synpcc7002_A2062 Elongation factor G 5.33E03 1.0380.00945* Constitutive (3.95E06) Constitutive (0.0392) 0.0315 Synpcc7002_A2127 Acetyl-CoA carboxylase biotin carboxylase subunit 3.62E04 1.0730.0576* Constitutive (1.64E06) Constitutive (0.0583) 0.457 Synpcc7002_A2165 Hypothetical protein 9.95E05 1.2050.129* Stationary phase (3.20E05) Constitutive (0.148) 0.960 Synpcc7002_A2210 C-phycocyanin subunit alpha 2.91E02 1.1290.191 Linear...”
PYCA_METJA / Q58626 Pyruvate carboxylase subunit A; Pyruvic carboxylase A; EC 6.4.1.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
50% identity, 88% coverage
AKJ12_RS08585 acetyl-CoA carboxylase biotin carboxylase subunit from Xanthomonas arboricola pv. juglandis
53% identity, 98% coverage
- Proteome Analysis of Walnut Bacterial Blight Disease
H, International journal of molecular sciences 2020 - “...(AKJ12_RS18195, AKJ12_RS14795, AKJ12_RS12205, and AKJ12_RS12930); four aminopeptidases (AKJ12_RS15095, AKJ12_RS13855, AKJ12_RS05625, and AKJ12_RS04085); and biotin carboxylase (AKJ12_RS08585). In addition, we highlighted argininosuccinate synthase (AKJ12_RS01860); lytic murein transglucosylase (AKJ12_RS06545); two copper-binding proteins located adjacent to each other in the Xaj genome; and a copper chaperone (AKJ12_RS00535, AKJ12_RS00530, and...”
ABO_2010 acetyl-CoA carboxylase, biotin carboxylase from Alcanivorax borkumensis SK2
52% identity, 97% coverage
Rru_A2435 Acetyl-CoA carboxylase, biotin carboxylase from Rhodospirillum rubrum ATCC 11170
Q2RRL0 Biotin carboxylase from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)
53% identity, 97% coverage
Q12EJ0 biotin carboxylase (EC 6.3.4.14) from Polaromonas sp. (see paper)
51% identity, 98% coverage
FXV78_RS03210 acetyl-CoA carboxylase biotin carboxylase subunit from Mediterraneibacter gnavus ATCC 29149
52% identity, 97% coverage
HSM_1606 acetyl-CoA carboxylase biotin carboxylase subunit from Haemophilus somnus 2336
51% identity, 98% coverage
MBHS_02490 acetyl-CoA carboxylase biotin carboxylase subunit from Candidatus Venteria ishoeyi
51% identity, 97% coverage
PM1091 AccC from Pasteurella multocida subsp. multocida str. Pm70
51% identity, 98% coverage
- Isolation and sequencing of a temperate transducing phage for Pasteurella multocida
Campoy, Applied and environmental microbiology 2006 - “...this work. Strain Relevant features Source PM108 PM403 PM1090 PM1091 PM1092 PM1093 Wild type and lysogenic for F108 Wild type and F108 sensitive Like PM403, but...”
- “...b Multiplicity of infection Transduction frequency b, c PM1091 PM1092 PM1093 PM403 PM403 PM403 rpsL rpoB gyrA 1543395 to 1543769 1960620 to 1956592 991251...”
VP2881 acetyl-CoA carboxylase, biotin carboxylase from Vibrio parahaemolyticus RIMD 2210633
52% identity, 97% coverage
lmo1357 acetyl-CoA carboxylase subunit (biotin carboxylase subunit) from Listeria monocytogenes EGD-e
LM6179_2100 acetyl-CoA carboxylase biotin carboxylase subunit from Listeria monocytogenes 6179
49% identity, 96% coverage
- SecA2 Associates with Translating Ribosomes and Contributes to the Secretion of Potent IFN-β Inducing RNAs
Teubner, International journal of molecular sciences 2022 - “...metabolism 0.09 0.13 lmo2206 clpB Chaperone protein ClpB Posttranslational modification. protein turnover. chaperones 0.12 0.13 lmo1357 acetyl-CoA carboxylase biotin carboxylase subunit Lipid transport and metabolism 0.12 0.11 lmo1217 Putative aminopeptidase. M42 family Carbohydrate transport and metabolism 0.09 0.11 lmo1928 aroF Chorismate synthase Amino acid transport and...”
- Prebiotic Oligosaccharides Potentiate Host Protective Responses against L. Monocytogenes Infection
Chen, Pathogens (Basel, Switzerland) 2017 - “...0.008 lmo1164, lmo1181 Adenosylcobalamin salvage from cobalamin 0.008 Biotin-carboxyl carrier protein assembly 0.022 accD , lmo1357 Vitamin B 6 Biosynthesis 0.022 serC , lmo2101 Amino acid metabolism Amino Acids Degradation 0.006 gltD, aspB, lmo2836, lmo2363, lmo0383, lmo1182, lmo2101, lmo2749, lmo1915 Proteinogenic Amino Acids Degradation 0.006 L-aspartate...”
- Listeria monocytogenes {sigma}B has a small core regulon and a conserved role in virulence but makes differential contributions to stress tolerance across a diverse collection of strains
Oliver, Applied and environmental microbiology 2010 - “...lmo0958 lmo0959 lmo1076 lmo1237 lmo1255 lmo1293 lmo1348 lmo1357 lmo1389 lmo1390 lmo1391 lmo1538 lmo1539 lmo1542 lmo1570 lmo1658 lmo1849 lmo1956 lmo1998 lmo2000...”
- Transcriptome analysis of Listeria monocytogenes exposed to biocide stress reveals a multi-system response involving cell wall synthesis, sugar uptake, and motility
Casey, Frontiers in microbiology 2014 - “...carboxylase (carboxyltransferase alpha subunit) 2.37 LM6179_2099 accB Acetyl-CoA carboxylase subunit (biotin carboxyl carrier subunit) 1.79 LM6179_2100 accC Acetyl-CoA carboxylase subunit (biotin carboxylase subunit) 3.52 LM6179_2324 accD Acetyl-CoA carboxylase (carboxyltransferase beta subunit) 2.02 LM6179_2580 fapR Transcription factor controlling fatty acid and phospholipid metabolism 3.89 A gene is...”
B0MI45 Biotin carboxylase from Anaerostipes caccae (strain DSM 14662 / CCUG 47493 / JCM 13470 / NCIMB 13811 / L1-92)
51% identity, 97% coverage
- Environmentally Relevant Concentration of Bisphenol S Shows Slight Effects on SIHUMIx
Schäpe, Microorganisms 2020 - “...a subunit of an Acetyl-CoA carboxylase and a phosphoglucosamine mutase from A. caccae (Uniprot accession B0MI45 and B0MGA4), involved in fatty acid synthesis as well as peptidoglycan and lipopolysaccharide (LPS) synthesis, respectively. Also three transporters, two assigned to B. thetaiotaomicron (Uniprot accession Q8A992 and Q8A991), one...”
SPy1745 putative acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus pyogenes M1 GAS
52% identity, 97% coverage
NTHI1145 acetyl-CoA carboxylase biotin carboxylase subunit from Haemophilus influenzae 86-028NP
51% identity, 97% coverage
D7DR99 Acetyl-CoA carboxylase, biotin carboxylase from Methanococcus voltae (strain ATCC BAA-1334 / A3)
47% identity, 89% coverage
AccC / b3256 biotin carboxylase (EC 6.3.4.14) from Escherichia coli K-12 substr. MG1655 (see 6 papers)
accC / P24182 biotin carboxylase (EC 6.3.4.14) from Escherichia coli (strain K12) (see 2 papers)
ACCC_ECOLI / P24182 Biotin carboxylase; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Escherichia coli (strain K12) (see 3 papers)
P24182 biotin carboxylase (EC 6.3.4.14) from Escherichia coli (see 7 papers)
fabE / AAA23409.1 biotin carboxylase from Escherichia coli (see 4 papers)
ECOLIN_RS18665 acetyl-CoA carboxylase biotin carboxylase subunit from Escherichia coli Nissle 1917
NP_417722 biotin carboxylase from Escherichia coli str. K-12 substr. MG1655
b3256 acetyl-CoA carboxylase from Escherichia coli str. K-12 substr. MG1655
51% identity, 97% coverage
- function: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
subunit: Acetyl-CoA carboxylase is a heterohexamer of biotin carboxyl carrier protein, biotin carboxylase and the two subunits of carboxyl transferase in a 2:2 complex. - Identification of a gene cluster for D-tagatose utilization in Escherichia coli B2 phylogroup
Ha, iScience 2022 - “...D-tagatose catabolism. The candidate region was completely absent in K-12 and was located between accC (ECOLIN_RS18665) encoding biotin carboxylase and yhdT (ECOLIN_RS18710) encoding a conserved inner membrane protein ( Figure2 A). Figure2 Gene cluster for D-tagatose degradation in E.coli Nissle 1917 (EcN) (A) Genetic organization of...”
- Cyclophilin PpiB is involved in motility and biofilm formation via its functional association with certain proteins.
Skagia, Genes to cells : devoted to molecular & cellular mechanisms 2016 (PubMed)- GeneRIF: For DnaK, acetyl-CoA carboxylase, biotin carboxylase subunit (AccC) and phosphate acetyltransferase (Pta) we also showed a direct role of PpiB in the functional control of these proteins because it increased the measured enzyme activity of each protein and further interfered with DnaK localization and the correct folding of AccC.
- Evidence against translational repression by the carboxyltransferase component of Escherichia coli acetyl coenzyme A carboxylase.
Smith, Journal of bacteriology 2014 - GeneRIF: This study reports that increased levels of carboxyltransferase (CT) tetramer have no detectable effect on translation of the CT subunit mRNAs.
- Dimerization of the bacterial biotin carboxylase subunit is required for acetyl coenzyme A carboxylase activity in vivo.
Smith, Journal of bacteriology 2012 - GeneRIF: It was concluded that biotin carboxylase must be dimeric to fulfill its physiological function.
- Crystal structure of biotin carboxylase in complex with substrates and implications for its catalytic mechanism.
Chou, The Journal of biological chemistry 2009 - GeneRIF: analysis of biotin carboxylase in complex with substrates and implications for its catalytic mechanism
- Coordinate expression of the acetyl coenzyme A carboxylase genes, accB and accC, is necessary for normal regulation of biotin synthesis in Escherichia coli.
Abdel-Hamid, Journal of bacteriology 2007 - GeneRIF: Results show that under AccC overexpression conditions AccB is underbiotinylated.
- The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase.
Li, The Journal of biological chemistry 1992 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on complete protein
- Structural assembly of the bacterial essential interactome
Gómez, eLife 2024 - “...structures are AlphaFold2 (AF2) models. Uniprot codes used for AF2: AccA: P0ABD5, AccB: P0ABD8, AccC: P24182, AccD: P0A9Q5, and birA: P06709. Figure 3figure supplement 1. Predicted interfaces of FabG 2 -AcpP 2 ( a ) and FabI 2 -AcpP 2 ( b ). The experimentally solved...”
- Toward Single Bacterium Proteomics
Végvári, Journal of the American Society for Mass Spectrometry 2023 - “...tufB P0CE48 [239249] [K].VGEEVEIVGIK.[E] 1629.98 544.00 +3 75.80 15 203.77 75.00% 11 biotin carboxylase accC P24182 [315324] [R].IAAGQPLSIK.[Q] 1456.91 728.96 +2 71.29 2 147.8 12.50% 120 probable cyclic di-GMP phosphodiesterase PdeC pdeC P32701 [3643] [K].SEVNNQLR.[T] 1189.64 595.33 +2 41.77 4 177.29 25.00% succinylornithine transaminase astC P77581...”
- “...atpA P0ABB0 [165175] [R].ELIIGDRQTGK.[T] 1229.68 615.35 +2 30.30 1 228.28 0.00% 33 biotin carboxylase accC P24182 [315324] [R].IAAGQPLSIK.[Q] 998.59 499.80 +2 49.58 1 181.48 0.00% 120 chaperone protein DnaK dnaK P0A6Y8 [167183] [K].RIINEPTAAALAYGLDK.[G] 1816.00 606.01 +3 64.08 1 285.59 0.00% 15 chromomal replication initiator protein DnaA...”
- Comprehensively Characterizing the Thioredoxin Interactome In Vivo Highlights the Central Role Played by This Ubiquitous Oxidoreductase in Redox Control
Arts, Molecular & cellular proteomics : MCP 2016 - “...P0A7A9 P0AC38 P0ABK5 P0A9C5 P0A825 P0A6B7 P0A817 P0A853 P24182 P0A6Q3 P0A953 P0AAI9 P0A6R0 P0A6Q6 P0ABF6 P76440 P0A717 P0A7D4 P07650 P0AEZ9 P0ACD4 Description...”
- A curated C. difficile strain 630 metabolic network: prediction of essential targets and inhibitors.
Larocque, BMC systems biology 2014 - “...sequence identity Target (pair) a Sequence identity b DrugBank potential inhibitors c E.coli Human accC P24182 [53%] Q96RQ3 [48%] LZJ, LZK, LZL, OA1, OA2, OA3, OA4, OA5, L21, L22, L23 (P24182 [53%]); BTI (Q99UY8 [48%]); BTN (P11498 [45%]); D1L, H1L (O00763 [32%]) adk P69441 [52%] P54819-2...”
- “...PLP , THL (P34897 [41%]); Mimosine (Pr4896 [40%]) CD0727 (folD | fhs | fchA) d P24182 [53%] Q96RQ3 [48%] CNC , THL , I2A (Q99707 [31%]) scoB (folD | fhs | fchA) d E9YQ86 [56%] B7Z609 [51%] SIN , EMT (P55809 [52%]) scoA (folD | fhs...”
- The Escherichia coli proteome: past, present, and future prospects
Han, Microbiology and molecular biology reviews : MMBR 2006 - “...of acetyl-CoA carboxylase 4.66/16,687.21 AccC AccD P24182 P0A9Q5 6.65/49,320.74 7.58/33,321.89 AceA P0A9G6 Biotin carboxylase Acetyl-CoA carboxylase carboxyl...”
- Tracing the phylogenetic history of the Crl regulon through the Bacteria and Archaea genomes
Santos-Zavaleta, BMC genomics 2019 - “...[ 11 ] lipid metabolic process, fatty acid metabolic process, fatty acid biosynthetic process accC b3256 accBC AccB (), FadR(+) + GEA and IMP [ 11 ] lipid metabolic process, fatty acid metabolic process, fatty acid biosynthetic process, metabolic process, negative regulation of fatty acid biosynthetic...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b2316 b2312, b2476 b4013 b3957 b4160 b2323 b2499, b2507 s0001 b3958 b3255 b2557, b2780 b3959 b3256 b3642, b4005 b3960 b4006, b4177 s0001 b4244, b4245 SS NSP CAC GSM TTPM TIM genes b0639 b0720 b0907 b0908, b1260 b0914 b1098 b1136 b1261, b1262 s0001 b2827 b1263, b1264 b3648...”
- Analysis of promoter targets for Escherichia coli transcription elongation factor GreA in vivo and in vitro
Stepanova, Journal of bacteriology 2007 - “...ribosomal subunit protein L13 accB b3255 1.9 accC b3256 1.8 Acetyl-CoA carboxylase, BCCP (biotin carboxyl carrier protein) subunit; carrier of biotin Acetyl-CoA...”
- Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12
Smulski, Journal of bacteriology 2001 - “...exbD fecA fecI fecR fepB fhuE fimA fis fixX flgD b3256 b4015 b4016 b1623 b0111 b0050 b0564 b0469 b0908 b2601 b1704 b3433 b0930 b1597 b3734 b3731 b3732 b3736...”
plu4075 biotin carboxylase (A subunit of acetyl-CoA carboxylase) (ACC) from Photorhabdus luminescens subsp. laumondii TTO1
51% identity, 97% coverage
Q06862 Biotin carboxylase from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)
49% identity, 97% coverage
- Differential protein profiling of soil diazotroph Rhodococcus qingshengii S10107 towards low-temperature and nitrogen deficiency.
Suyal, Scientific reports 2019 - “...kinase, B8HVB6), ThyA (Thymidylate synthase, Q0SEI1), RpsF (30S ribosomal protein S6), Tkt, AccC (Biotin carboxylase, Q06862), PanD (Aspartate 1-decarboxylase, Q8YR79), HutI (Imidazolonepropionase, B2JCJ1), PckG, SucC, Eno , Urease accessory proteins, glmM (Phosphoglucosamine mutase, Q98F91), GlmS, DdlA, Dxr (1-deoxy-D-xylulose 5-phosphate reductoisomerase, B7K5G6), Mqo (Probable malate:uinine oxidoreductase, Q89XM4),...”
- “...Glutamine fructose-6-phosphate aminotransferase, P59362 ); Biotin metabolism ( ATP-dependent dethiobiotin synthetase, B7K5E6 and Biotin carboxylase, Q06862 ), Nitrogen metabolism (Urease enzyme complex) and Nucleotide metabolism ( dITP/XTP pyrophosphatase, Q98DN4 and Thymidylate synthase, Q0SEI1 ) while down-regulating the enzymes for Lipid biosynthesis ( Acyl-[acyl-carrier-protein] UDP-N-acetylglucosamine O-acyltransferase, A8I491...”
llmg_1779 acetyl-CoA carboxylase biotin carboxylase subunit from Lactococcus lactis subsp. cremoris MG1363
52% identity, 96% coverage
- Mechanisms of Acetoin Toxicity and Adaptive Responses in an Acetoin-Producing Species, Lactococcus lactis
Cesselin, Applied and environmental microbiology 2021 - “...a Expression change in acetoin Glycolysis llmg_2539 GapB glyceraldehyde 3-phosphate dehydrogenase Down Fatty acid biosynthesis llmg_1779 AccC subunit biotin carboxylase Down llmg_1783 FabF 3-oxoacyl-acyl carrier protein synthase II Down llmg_0538 FabZ (a) 3-hydroxy-acyl-[acyl-carrier-protein] dehydratase Down Cell wall llmg_0209 RmlB, dTDP-glucose-1-phosphate dehydratase Down llmg_0210 RmlD, dTDP-4-dehydrorhamnose reductase...”
c4012 acetyl-CoA carboxylase biotin carboxylase subunit from Escherichia coli CFT073
51% identity, 97% coverage
ECs4128 acetyl CoA carboxylase biotin carboxylase subunit from Escherichia coli O157:H7 str. Sakai
51% identity, 97% coverage
Q8DSN9 Biotin carboxylase from Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
SMU_RS07890 acetyl-CoA carboxylase biotin carboxylase subunit from Streptococcus mutans UA159
52% identity, 97% coverage
- S-glutathionylation proteome profiling reveals a crucial role of a thioredoxin-like protein in interspecies competition and cariogenecity of Streptococcus mutans
Li, PLoS pathogens 2020 - “...[glutamine-hydrolyzing] Q8DRY2 nrdD 625 Putative anaerobic ribonucleoside-triphosphate reductase P26283 Pyruvate metabolism ldh 74 L-lactate dehydrogenase Q8DSN9 accC 95, 338 Putative acetyl-CoA carboxylase biotin carboxylase subunit Q8DTF8 pckA 236, 324 Phosphoenolpyruvate carboxykinase Q59939 Carbon metabolism citZ 13 Citrate synthase P72484 tpiA 127, 128, 188 Triosephosphate isomerase Q8DVV3...”
- Mass spectrometry and split luciferase complementation assays reveal the MecA protein interactome of Streptococcus mutans
Qin, Microbiology spectrum 2024 - “...5.5 Cysteine desulfurase Fatty acid biosynthesis SMU_RS07880 2.8 Acetyl-CoA carboxylase carboxyl transferase subunit alpha, AccA SMU_RS07890 2.6 Acetyl-CoA carboxylase biotin carboxylase subunit, AccC SMU_RS07910 2.5 3-oxoacyl-[acyl-carrier-protein] reductase, FabG SMU_RS07905 2.2 Beta-ketoacyl-ACP synthase II, FabF SMU_RS07915 2 2 ACP S-malonyltransferase, FabD Lipid metabolism SMU_RS01590 2.8 NAD(P)H-dependent glycerol-3-phosphate...”
D8UF54 Biotin carboxylase from Volvox carteri f. nagariensis
52% identity, 85% coverage
6ojhA / P43873 Crystal structure of haemophilus influenzae biotin carboxylase complexed with (r)-7-(3-aminopyrrolidin-1-yl)-6-(naphthalen-1-yl) pyrido[2,3-d]pyrimidin-2-amine
51% identity, 97% coverage
- Ligands: 7-[(3r)-3-aminopyrrolidin-1-yl]-6-(naphthalen-1-yl)pyrido[2,3-d]pyrimidin-2-amine; calcium ion (6ojhA)
1dv2A / P24182 The structure of biotin carboxylase, mutant e288k, complexed with atp (see paper)
51% identity, 97% coverage
- Ligand: adenosine-5'-triphosphate (1dv2A)
P43873 biotin carboxylase (EC 6.3.4.14) from Haemophilus influenzae (see paper)
HI0972 acetyl-CoA carboxylase, biotin carboxylase (accC) from Haemophilus influenzae Rd KW20
51% identity, 97% coverage
F502_10483 acetyl-CoA carboxylase biotin carboxylase subunit from Clostridium pasteurianum DSM 525 = ATCC 6013
49% identity, 96% coverage
Z4616 acetyl CoA carboxylase, biotin carboxylase subunit from Escherichia coli O157:H7 EDL933
51% identity, 97% coverage
STM3380 acetyl CoA carboxylase, biotin carboxylase subunit from Salmonella typhimurium LT2
51% identity, 97% coverage
APL_1865 Biotin carboxylase from Actinobacillus pleuropneumoniae L20
50% identity, 97% coverage
Q10YA8 biotin carboxylase (EC 6.3.4.14) from Trichodesmium erythraeum (see paper)
50% identity, 98% coverage
VFMJ11_2506 acetyl-CoA carboxylase biotin carboxylase subunit from Aliivibrio fischeri MJ11
VFMJ11_2506 acetyl-CoA carboxylase biotin carboxylase subunit from Vibrio fischeri MJ11
52% identity, 97% coverage
Clocel_4136 acetyl-CoA carboxylase biotin carboxylase subunit from Clostridium cellulovorans 743B
48% identity, 98% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...chain amino acids) biosynthesis and proteins involved in cell envelope re-arrangement (e.g., the products of Clocel_4136, Clocel_4137, Clocel_4144, Clocel_4162 and Clocel_4352, involved in the biosynthesis of saturated fatty acids) and a redistribution of carbon flux through fermentative pathways (acetate and formate yields were increased and decreased,...”
- “...). In the present study, five proteins involved in the biosynthesis of saturated fatty acids (Clocel_4136, Clocel_4137, Clocel_4144, Clocel_4162, and Clocel_4352) and two enzymes likely involved in phospholipid biosynthesis (Clocel_1331, Clocel_1338) were identified among the proteins up-regulated by butanol. Curiously, one of the two acyl carrier...”
F6H9P9 Biotin carboxylase from Vitis vinifera
50% identity, 84% coverage
- Root Proteomic Analysis of Two Grapevine Rootstock Genotypes Showing Different Susceptibility to Salt Stress
Prinsi, International journal of molecular sciences 2020 - “...11, 21) 26.09 Lipid metabolism (11) 100 F6HXC8 Phospholipase D (11, 1, 27) 3.90 41 F6H9P9 Biotin carboxylase 1, chloroplastic (11) 2.57 70 F6HLJ7 Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic (11) 2.13 182 D7STF0 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (11) 3.22 211 D7T4I1 Biotin carboxyl carrier protein of acetyl-CoA...”
- “...reductase (11) 1.42 60 F6HLJ7 Enoyl-[acyl-carrier-protein] reductase [NADH] 1, chloroplastic isoform X1 (11) 1.68 65 F6H9P9 Biotin carboxylase 1, chloroplastic () 1.76 168 D7STF0 3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabZ (11) 1.94 203 D7T4I1 Biotin carboxyl carrier protein of acetyl-CoA carboxylase 2, chloroplastic (11) 2.98 88 D7TAP7 3-oxoacyl-[acyl-carrier-protein] reductase...”
- Differential proteomic analysis of grapevine leaves by iTRAQ reveals responses to heat stress and subsequent recovery
Liu, BMC plant biology 2014 - “...1.095 6.3 Ricinus communis Malate dehydrogenase A5BEJ8 0.524 1.328 6.3 Vitis vinifera Malate dehydrogenase, putative F6H9P9 0.455 1.618 11.1.1 Camellia oleifera Biotin carboxylase, CAC2 G3G8J7 0.425 0.696 12.1.2 Vitis vinifera Nitrite reductase 1 D7SW04 0.744 0.519 13.1.1.2.1 Petunia x hybrida Prephenate aminotransferase A5ACX0 0.389 1.125 13.1.1.3.1...”
HD0635 biotin carboxylase from Haemophilus ducreyi 35000HP
50% identity, 97% coverage
ECA0260 acetyl-CoA carboxylase biotin carboxylase subunit from Pectobacterium atrosepticum SCRI1043
50% identity, 97% coverage
- Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum
Gorshkov, PloS one 2017 - “...ECA1166, ECA1540, ECA2013, ECA2873, ECA2226; down-regulatedECA3054, ECA3682, ECA4006, ECA4513, ECA1141, ECA1232, ECA4461, ECA1407, ECA2651, ECA4079, ECA0260, ECA0255, ECA0238, ECA3343, ECA3814. Total RNA was extracted from Pba cells and digested with DNAze as described above and used for cDNA synthesis. The reaction mixture for reverse transcription contained...”
lpg2664 acetyl-CoA carboxylase, biotin carboxylase subunit from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
49% identity, 94% coverage
- New Global Insights on the Regulation of the Biphasic Life Cycle and Virulence Via ClpP-Dependent Proteolysis in Legionella pneumophila
Ge, Molecular & cellular proteomics : MCP 2022 - “..., ClpA, GrpE, DnaJ, ClpB, ClpS), and microbial metabolism in diverse environments ( e.g. , Lpg2664, Lpg0245, AcsB, AtoB) ( supplemental Fig.S4 C ). In addition, the downregulated proteins in the RP of the clpP strain were found to interact with ribosomal proteins ( e.g. ,...”
- “...network analysis showed that the key proteins involved in the TP to RP transition, including Lpg2664, AcsB, MmsA, AtoB, FliA, FliS, FlhF, Frr, Lpg0358, and Lpg2228, presented decreased abundance, whereas Lpg0102, Lpg0361, Lpg0362, FabZ, RpmA, RplS, RpsP, RpsH, Lpg0394, and FtsQ increased in abundance ( supplemental...”
G7LIV6 Biotin carboxylase from Medicago truncatula
50% identity, 81% coverage
F2WMV4 biotin carboxylase (EC 6.3.4.14) from Jatropha curcas (see paper)
49% identity, 85% coverage
YPO3658 biotin carboxylase from Yersinia pestis CO92
YPTB3572 biotin carboxylase from Yersinia pseudotuberculosis IP 32953
51% identity, 97% coverage
Ngar_c01880 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Candidatus Nitrososphaera gargensis Ga9.2
46% identity, 89% coverage
CAC2 / O04983 biotin carboxylase (EC 6.3.4.14) from Arabidopsis thaliana (see paper)
ACCC_ARATH / O04983 Biotin carboxylase, chloroplastic; Acetyl-coenzyme A carboxylase biotin carboxylase subunit A; EC 6.3.4.14 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
AT5G35360 CAC2; acetyl-CoA carboxylase/ biotin carboxylase from Arabidopsis thaliana
NP_198386 acetyl Co-enzyme a carboxylase biotin carboxylase subunit from Arabidopsis thaliana
50% identity, 83% coverage
- function: This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
subunit: Acetyl-CoA carboxylase is a heterohexamer composed of biotin carboxyl carrier protein, biotin carboxylase and two subunits each of ACCase subunit alpha and ACCase plastid-coded subunit beta (accD). - Bioinformatics Identification and Expression Analysis of Acetyl-CoA Carboxylase Reveal Its Role in Isoflavone Accumulation during Soybean Seed Development
Wu, International journal of molecular sciences 2024 - “...is a hetero-oligomer composed of four different subunits located in the plastids ( AT5G16390 , AT5G35360 , AT2G38040 , and ATCG00500 ), while the other two are homodimers, ACC1 ( AT1G36160 ) and ACC2 ( AT1G36180 ), located in the cytoplasm and chloroplasts [ 22 ]....”
- Circadian clock factors regulate the first condensation reaction of fatty acid synthesis in Arabidopsis
Kim, Cell reports 2023 - “...CCA1, At2g46830; TOC1, At5g61380; ACC1, At1g36160; ACC2, At1g36180; KASI, At5g46290; KASII, At1g74960; KASIII, At1g62640; BC, At5g35360; BCCP1, At5g16390; BCCP2, At5g15530; BADC1, At3g56130; BADC2, At1g52670; BADC3, At3g15690; a-CT, At2g38040; b-CT, Atcg00500; Ubiquitin10, At4g05320. QUANTIFICATION AND STATISTICAL ANALYSIS Statistical analyses were performed by two-tailed Students t-test using the...”
- Iron Availability Influences Protein Carbonylation in Arabidopsis thaliana Plants
Tola, International journal of molecular sciences 2023 - “...Chloroplast Signalling 23 Metacaspase-4 O64517 AT1G79340 45 2 Cytosol Plant defense 24 Biotin carboxylase F4JYE0 AT5G35360 61 9 Chloroplast Fatty acid metabolism 25 Formate dehydrogenase A0A1P8B9L1 AT5G14780 41 4 Mitochondria ijms-24-09732-t002_Table 2 Table 2 List of carbonylated proteins identified in the iron-deficient samples only. SN Protein...”
- An expanded role for the transcription factor WRINKLED1 in the biosynthesis of triacylglycerols during seed development
Kuczynski, Frontiers in plant science 2022 - “...Attachment Domain Containing, AT3G15690) 4 , 5 87/+73(+) 9.53.1 0.58 0.94 ** BC (Biotin Carboxylase, AT5G35360) 6 68/+62() 0.20.2 0.50 0.98 ** BCCP 1 (Biotin Carboxyl Carrier Protein 1, AT5G16390) 7 60/+43(+) 62.219.5 0.92 0.93 ** BCCP 2 (Biotin Carboxyl Carrier Protein 2, AT5G15530) 5 ,...”
- The Sunflower WRINKLED1 Transcription Factor Regulates Fatty Acid Biosynthesis Genes through an AW Box Binding Sequence with a Particular Base Bias
Sánchez, Plants (Basel, Switzerland) 2022 - “...Y HanXRQr2Chr15g0671211 -CT-15g 2 Plastid Y-5 D.N.S. -CT AtCg00500 HanXRQr2CPg0836391 0 Plastid Y-0.8 D.N.S. BC At5g35360 HanXRQr2Chr16g0770591 BC-16g 6 Plastid Y-35 D.N.S. HanXRQr2Chr17g0824731 0 Plastid Y-7 BCCP At5g15530 HanXRQr2Chr02g0061841 BCCP-2g 1 Plastid Y-18 D.N.S. HanXRQr2Chr05g0231761 0 Plastid Y-3 HanXRQr2Chr09g0408331 BCCP-9g 2 Plastid Y-85 HanXRQr2Chr16g0729021 BCCP-16g 6...”
- New Insights into the Chloroplast Outer Membrane Proteome and Associated Targeting Pathways
Fish, International journal of molecular sciences 2022 - “...Hsc70-1 At5g02580 * Argininosuccinate Lyase At5g16870 PTH2 Family Protein At5g20300 TOC90 At5g20410 MGD2 At5g23190 CYP86B1 At5g35360 CAC2/BC At5g42070 - At5g43070 WPP1 At5g53280 PDV1 At5g58140 PHOT2 At5g59840 RAB8A-Like Protein 1 Arabidopsis gene identifier (AGI) number, 2 Proteins without a name are marked with -, 3 N-Terminal transit...”
- Integrated Transcriptomic and Bioinformatics Analyses Reveal the Molecular Mechanisms for the Differences in Seed Oil and Starch Content Between Glycine max and Cicer arietinum
Cheng, Frontiers in plant science 2021 - “...BCCP1 Biotin Carboxyl Carrier Protein of Heteromeric ACCase Glyma.18G243500 Glyma.09G248900 Ca_06111 Klaus et al., 2004 AT5G35360 BC Biotin Carboxylase of Heteromeric ACCase Glyma.08G027600 Glyma.05G221100 Ca_05874 Roesler et al., 1997 AT5G46290 KASI Ketoacyl-ACP Synthase I Glyma.08G084300 Ca_05157 Yang et al., 2016 AT1G74960 KASII Ketoacyl-ACP Synthase II Glyma.17G047000...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...Biotin carboxyl carrier protein of acetyl-CoA carboxylase 1 86 Q42533, At5g16390 Biotin carboxylase 47 O04983, At5g35360 Enoyl-[acyl-carrier-protein] reductase [NADH] 82, 83 Q9SLA8, At2g05990 Pyruvate dehydrogenase E1 component subunit beta-3 79 O64688, At2g34590 ENERGETICS ATP synthesis ATP synthase subunit alpha 22, 30, 49, 86 P56757, AtCg00120 ATP...”
- “...- and -subunits of carboxyltransferase [ 50 ]. Indeed, three of these proteins (biotin carboxylase, At5g35360, spot 47; biotin carboxyl carrier protein, At5g16390, spot 86; carboxyl transferase subunit , AtCg00500, spots 47 and 59) were identified in our root plastid sample (Table 1 ). Then, another...”
- More
- Chloroplast acetyl-CoA carboxylase activity is 2-oxoglutarate-regulated by interaction of PII with the biotin carboxyl carrier subunit.
Feria, Proceedings of the National Academy of Sciences of the United States of America 2010 - GeneRIF: These data show that PII function has evolved between bacterial and plant systems to control the carbon metabolism pathway of fatty acid synthesis in plastids, through regulation of acetyl-CoA carboxylase activity.
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...AtCg00500 Biotin carboxyl carrier protein of acetyl-CoA carboxylase 1 86 Q42533, At5g16390 Biotin carboxylase 47 O04983, At5g35360 Enoyl-[acyl-carrier-protein] reductase [NADH] 82, 83 Q9SLA8, At2g05990 Pyruvate dehydrogenase E1 component subunit beta-3 79 O64688, At2g34590 ENERGETICS ATP synthesis ATP synthase subunit alpha 22, 30, 49, 86 P56757, AtCg00120...”
- Identification of the Candidate Proteins Related to Oleic Acid Accumulation during Peanut (Arachis hypogaea L.) Seed Development through Comparative Proteome Analysis
Liu, International journal of molecular sciences 2018 - “...1.23 Q9ZVQ3 GSTZ1 lipid oxidation Araip.10039882.1 1.17 Q8L9C4 KCR1 fatty acid biosynthetic process Araip.10034519.1 1.08 O04983 CAC2 fatty acid derivative biosynthetic process Araip.10005278.1 1.03 O04420 At2g26230 lipid oxidation Araip.10021913.1 1.01 Q9FMN0 SCP2 lipid oxidation Araip.10000513.1 1.04 Q38882 PLDALPHA1 fatty acid metabolic process Araip.10003126.1 1.04 O22898 LACS1...”
- “...Araip.10034912.1 1.25 Q9FLH8 At5g51830 lipid metabolic process Araip.10032969.1 1.23 Q9ZVQ3 GSTZ1 lipid oxidation Araip.10034519.1 1.13 O04983 CAC2 lipid biosynthetic process Araip.10000107.1 1.02 P56765 accD lipid biosynthetic process Araip.10036992.1 1 Q9CAP8 LACS9 fatty acid metabolic process Araip.10004872.1 1.04 O65202 ACX1 lipid oxidation Araip.10003126.1 1.11 O22898 LACS1 fatty...”
- dEMBF: A Comprehensive Database of Enzymes of Microalgal Biofuel Feedstock
Misra, PloS one 2016 - “...carboxylase alpha-carboxyltransferase alpha-CT 6.4.1.2 Q9LD43 Acetyl-CoA carboxylase beta-carboxyltransferase beta-CT 6.4.1.2 P56765 Biotin carboxylase BC 6.3.4.14 O04983 Biotin carboxyl carrier protein BCCP 6.4.1.2 Q9LLC1, Q42533 Malonyl-CoA-ACP Malonyltransacylase MCMT/MCAT/FabD 2.3.1.39 Q8RU07 beta-ketoacyl-ACP Synthase I KAS I/FabF 2.3.1.179 P52410 beta-ketoacyl-ACP Synthase II KAS II/FabF 2.3.1.179 Q9C9P4 beta-ketoacyl-ACP Synthase III...”
- Microgravity induces changes in microsome-associated proteins of Arabidopsis seedlings grown on board the international space station
Mazars, PloS one 2014 - “...Early nodulin-like protein 2 (Phytocyanin-like protein) Lipid metabolism F4HYI6 AT1G48600 0.56281 Putative phosphoethanolamine N-methyltransferase 2 O04983 AT5G35360 0.52901 Biotin carboxylase, chloroplastic (EC 6.3.4.14) (Acetyl-CoA carboxylase subunit A) (ACC) (EC 6.4.1.2) Q9LD43 AT2G38040 0.33612 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha, chloroplastic (ACCase subunit alpha) (EC 6.4.1.2)...”
- Phylogenomic study of lipid genes involved in microalgal biofuel production-candidate gene mining and metabolic pathway analyses
Misra, Evolutionary bioinformatics online 2012 - “...Q00ZG8 CMM188C 41 46 Heteromeric ACC biotin carboxylase subunit (BCC) 6.4.1.2/6.3.4.14 KOG0238 K01961 GO:0004075 GO:0003989 O04983, F4JYE1, F4JYE0 A8JGF4, A8JEW0 D8UF54 A4S140 , Q013U7 CMS299C ACC carboxyl-transferase -subunit (ACCCT ) 6.4.1.2 KOG0238 K01962 GO:0003989 Q9LD43 A8J646 D8TNY0 CMV056C ACC CT subunit (ACCCT ) 6.4.1.2 KOG0540 K01963...”
B9S1E2 Biotin carboxylase from Ricinus communis
50% identity, 82% coverage
F4JYE0 Biotin carboxylase from Arabidopsis thaliana
50% identity, 80% coverage
pycA / O27939 pyruvate carboxylase subunit A (EC 6.4.1.1) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (see paper)
46% identity, 90% coverage
O23960 Biotin carboxylase from Glycine max
50% identity, 81% coverage
- Redox Conformation-Specific Protein-Protein Interactions of the 2-Cysteine Peroxiredoxin in Arabidopsis
Liebthal, Antioxidants (Basel, Switzerland) 2020 - “...chain 2 Q43832 * Malate dehydrogenase [NADP] P52426 * Glutamate--ammonia ligase Q9LVI8 * Biotin carboxylase O23960 2Cys-peroxiredoxin Q9SQJ4...”
- Proteomics gives insight into the regulatory function of chloroplast thioredoxins
Balmer, Proceedings of the National Academy of Sciences of the United States of America 2003 - “...are O20252, P09559, P12860, P10871, Q43832, P52426, Q9LVI8, O23960, and Q9SQJ4. *See Table 2 for definition of numbers. eluant from the thioredoxin columns....”
BCAL3421 biotin carboxylase from Burkholderia cenocepacia J2315
49% identity, 99% coverage
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction
Fang, BMC systems biology 2011 - “...analysis, the genome-scale metabolic model of B. cenocepacia was further refined. For example, BCAL0660 and BCAL3421, which were homologous genes encoding protein AccC according to their annotation, were originally both included in the model with the gene-protein-reaction (GPR) relationship of "BCAL0660 or BCAL3421". Through in silico...”
- “...are cotranscribed and form an operon together [ 73 , 74 ]. Furthermore, BCAL3420 and BCAL3421 shows greater than 2-fold expression for J2315 under CF conditions versus soil conditions, yet BCAL0660 shows an opposite result [ 75 ]. Taken together, BCAL0660 was excluded from model i...”
A4SVB6 biotin carboxylase (EC 6.3.4.14) from Polynucleobacter asymbioticus QLW-P1DMWA-1 (see paper)
50% identity, 98% coverage
CDR20291_1861 biotin carboxylase (acetyl-CoA carboxylase subunit A) from Clostridium difficile R20291
CDR20291_1861 acetyl-CoA carboxylase biotin carboxylase subunit from Clostridioides difficile R20291
46% identity, 98% coverage
- Clostridioides difficile LuxS mediates inter-bacterial interactions within biofilms
Slater, Scientific reports 2019 - “...1.7011992 3-oxoacyl-ACP synthase III 4 CDR20291_1018 1.2469326 trans-2-enoyl-ACP reductase 5 CDR20291_1337 1.3290499 transcriptional regulator 6 CDR20291_1861 1.412304 biotin carboxylase acetyl-CoA carboxylase subunit A 7 CDR20291_2027 1.6309458 2-nitropropane dioxygenase 8 CDR20291_3225 1.0229025 formate/nitrite transporter 9 CDR20291_0363 1.377088 radical SAM protein 10 CDR20291_0364 1.003595 hypothetical protein 11 CDR20291_0365...”
Q499_0133 acetyl-CoA carboxylase biotin carboxylase subunit from Chlamydia suis MD56
49% identity, 97% coverage
CPS_RS04200 acetyl-CoA carboxylase biotin carboxylase subunit from Colwellia psychrerythraea 34H
50% identity, 97% coverage
O67449 Biotin carboxylase from Aquifex aeolicus (strain VF5)
aq_1470 biotin carboxylase from Aquifex aeolicus VF5
47% identity, 94% coverage
CT124 Biotin Carboxylase from Chlamydia trachomatis D/UW-3/CX
48% identity, 97% coverage
- Remodeling of host phosphatidylcholine by Chlamydia acyltransferase is regulated by acyl-CoA binding protein ACBD6 associated with lipid droplets
Soupene, MicrobiologyOpen 2015 - “...and the common extender molecule, malonyl-CoA, is produced by AccA, AccB, AccC, and AccD (CT123, CT124, CT265, CT293). The condensation/elongation reaction is also common to both pathways and is performed by FabH (CT239) and FabF (CT770). No fatty acid activating enzyme, long-chain acyl-CoA synthetase (FadD), is...”
- Chlamydia trachomatis infection induces replication of latent HHV-6
Prusty, PloS one 2013 - “...Blood virus load/10 3 cells 1 CT100 <32 ND ND ND (<5) 275809.309 7.99 2 CT124 128 ND ND ND (<5) 17020.3654 21.94766605 3 CT127 <32+/ ND ND ND (<5) 28133.5987 6.04619509 4 CT129 128 ND ND ND (<5) 20731.6709 6.483198133 5 CT133 64 80 ND...”
JYQ65_01920 acetyl-CoA carboxylase biotin carboxylase subunit from Limosilactobacillus fermentum
52% identity, 94% coverage
Pnuc_0913 acetyl-CoA carboxylase, biotin carboxylase from Polynucleobacter sp. QLW-P1DMWA-1
47% identity, 65% coverage
Cs308_0800 acetyl-CoA carboxylase biotin carboxylase subunit from Candidatus Chlamydia sanziniae
48% identity, 97% coverage
Dsui_0516 Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) from Dechlorosoma suillum PS
47% identity, 66% coverage
- mutant phenotype: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (PROPIONYL-COA-CARBOXY-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
MSMEG_4716 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Mycobacterium smegmatis str. MC2 155
53% identity, 66% coverage
F4JYE1 Biotin carboxylase from Arabidopsis thaliana
51% identity, 83% coverage
Mvan_4090 Carbamoyl-phosphate synthase L chain, ATP-binding from Mycobacterium vanbaalenii PYR-1
54% identity, 67% coverage
SSPN_RS0134935 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Saccharopolyspora spinosa NRRL 18395
54% identity, 97% coverage
1dv1B / P24182 Structure of biotin carboxylase (apo) (see paper)
48% identity, 97% coverage
- Ligand: phosphate ion (1dv1B)
Q1D0T9 Acetyl-CoA carboxylase, biotin carboxylase from Myxococcus xanthus (strain DK1622)
52% identity, 96% coverage
aq_1517 pyruvate carboxylase n-terminal domain from Aquifex aeolicus VF5
O67483 Pyruvate carboxylase n-terminal domain from Aquifex aeolicus (strain VF5)
51% identity, 93% coverage
G9LQ56 Acetyl-CoA carboxylase biotin carboxylase subunit from Bacillus subtilis subsp. subtilis
49% identity, 92% coverage
Sfum_0676 Carbamoyl-phosphate synthase L chain, ATP-binding from Syntrophobacter fumaroxidans MPOB
48% identity, 93% coverage
4rzqA / P43873 Structural analysis of substrate, reaction intermediate and product binding in haemophilus influenzae biotin carboxylase (see paper)
48% identity, 97% coverage
- Ligands: methyl (3as,4s,6ar)-4-(5-methoxy-5-oxopentyl)-2-oxohexahydro-1h-thieno[3,4-d]imidazole-1-carboxylate; adenosine-5'-diphosphate (4rzqA)
MMAR_3848 acetyl-/propionyl-coenzyme A carboxylase alpha chain AccA1 from Mycobacterium marinum M
52% identity, 67% coverage
BT1915 pyruvate carboxylase subunit A from Bacteroides thetaiotaomicron VPI-5482
44% identity, 87% coverage
- Gut Commensal Bacteroidetes Encode a Novel Class of Vitamin B12-Binding Proteins
Putnam, mBio 2022 - “...extracellular B 12 . Expression of the first gene downstream of a cobamide-dependent riboswitch ( bt1915 ) was assessed by qRT-PCR. Inactivation of cobamide transport locus 2 compromises B. thetaiotaomicron fitness in the mouse gut ( 5 , 12 ). In germfree Swiss Webster mice, parental...”
- “...of a predicted riboswitch decreases in the presence of B 12 . (A) The gene bt1915 is encoded immediately downstream from a predicted B 12 -responsive riboswitch. Using the B. thetaiotaomicron tdk strain, we assessed transcription of bt1915 in the presence of 0, 1, and 10...”
CAB540 biotin carboxylase from Chlamydophila abortus S26/3
48% identity, 97% coverage
D174_19730 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycolicibacterium neoaurum VKM Ac-1815D
52% identity, 67% coverage
BA2548 acetyl-CoA carboxylase, biotin carboxylase, putative from Bacillus anthracis str. Ames
46% identity, 98% coverage
Q93Y50 Biotin carboxylase from Brassica napus
49% identity, 83% coverage
pycA / Q6M0D0 pyruvate carboxylase subunit A (EC 6.4.1.1) from Methanococcus maripaludis (strain S2 / LL) (see paper)
MMP0341 pyruvate carboxylase subunit A from Methanococcus maripaludis S2
44% identity, 89% coverage
- The Sac10b homolog in Methanococcus maripaludis binds DNA at specific sites
Liu, Journal of bacteriology 2009 - “...0.11 MMP0092 2 1.66 0.10 MMP0148 3 1.36 0.12 MMP0276 MMP0341 MMP0414 MMP0594 2 4 6 2 1.57 1.26 1.51 1.53 0.06 0.07 0.03 0.11 MMP0684 MMP0872 MMP0971 MMP1157...”
- “...oxidoreductase subunit (Mmp0003), pyruvate carboxylase subunit A (Mmp0341), and acetyl-CoA synthetase (Mmp0148). While the differences in protein levels were...”
AYM39_19225 acetyl-CoA carboxylase biotin carboxylase subunit from Methylomonas sp. DH-1
50% identity, 93% coverage
Eab7_0871 acetyl-CoA carboxylase biotin carboxylase subunit from Exiguobacterium antarcticum B7
53% identity, 97% coverage
- Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data
Kawasaki, BioMed research international 2016 - “...EC number accA Eab7_2059 6.4.1.2 accA Eab7_2059 6.4.1.2 accB Eab7_0870 6.4.1.2 accB Eab7_0870 6.4.1.14 accC Eab7_0871 6.4.1.2 accC Eab7_0871 6.4.1.2 accD Eab7_2060 6.4.1.2 accD Eab7_2060 6.4.1.14 fabD Eab7_1760 2.3.1.39 fabD Eab7_1760 2.3.1.39 fabH1 Eab7_1911 2.3.1.180 fabH1 Eab7_1911 2.3.1.180 fabF Eab7_1910 2.3.1.179 fabF Eab7_1910 2.3.1.179 fabG Eab7_1795...”
- “...carrier protein ATP + acetyl-CoA + HCO 3 ADP + orthophosphate + malonyl-CoA 0.05773 accC Eab7_0871 6.4.1.2 6.4.1.14 Acetyl-CoA carboxylase, biotin carboxylase subunit ATP + acetyl-CoA + HCO 3 ADP + orthophosphate + malonyl-CoA 0.5623 accD Eab7_2060 6.4.1.2 Acetyl-CoA carboxylase carboxyl transferase beta subunit ATP +...”
pRL100436 putative biotin carboxylase from Rhizobium leguminosarum bv. viciae 3841
51% identity, 98% coverage
Q1D0B9 Biotin carboxylase from Myxococcus xanthus (strain DK1622)
50% identity, 98% coverage
- Proteome Analyses of Soil Bacteria Grown in the Presence of Potato Suberin, a Recalcitrant Biopolymer
Sidibé, Microbes and environments 2016 - “...did not provide sufficient energy for bacterial proliferation. The presence of several carboxylases (Q1CYB4, Q1DDA0, Q1D0B9, Q1DOT9, Q1D555, Q1DDA2, and Q1D8V2) in the Myxococcus proteome also supports this hypothesis. Carboxylation represents a cellular biosynthetic mechanism to replenish tricarboxylic acid cycle intermediates used in biosynthetic pathways (...”
- “...synthetase 0.06 I Q1DDA0 propionyl-CoA carboxylase 0.07 0.02 I Q1DFT0 3-oxoacyl-[acyl-carrier protein] reductase 0.21 I Q1D0B9 acetyl-CoA carboxylase 0.10 I Q1D340 malonyl CoA-acyl carrier protein transacylase 0.11 I Q1D5U1 3-hydroxyacyl-CoA dehydrogenase 0.18 I Q1D5U2 enoyl CoA dehydratase 0.13 I Q1D4E4 acyl-CoA dehydrogenase 0.07 I Q1D0T9 acetyl...”
SVEN_2566 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Streptomyces venezuelae ATCC 10712
51% identity, 69% coverage
MUL_3779 acetyl-/propionyl-coenzyme a carboxylase alpha chain AccA1 from Mycobacterium ulcerans Agy99
52% identity, 67% coverage
ACCA1_MYCTU / P9WPQ3 Biotin-dependent 3-methylcrotonyl-coenzyme A carboxylase alpha1 subunit; EC 6.3.4.14 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
CAA79609.1 biotin carboxyl carrier protein from Mycobacterium tuberculosis (see paper)
MT2576 acetyl/propionyl-CoA carboxylase, alpha subunit from Mycobacterium tuberculosis CDC1551
Rv2501c PROBABLE ACETYL-/PROPIONYL-COENZYME A CARBOXYLASE ALPHA CHAIN (ALPHA SUBUNIT) ACCA1: BIOTIN CARBOXYLASE + BIOTIN CARBOXYL CARRIER PROTEIN (BCCP) from Mycobacterium tuberculosis H37Rv
51% identity, 69% coverage
- function: Component of a biotin-dependent acyl-CoA carboxylase complex. This subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, resulting in the formation of carboxyl biotin (PubMed:25695631). When associated with the beta1 subunit AccD1, is involved in branched amino-acid catabolism with methylcrotonyl coenzyme A as the substrate (PubMed:25695631).
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The biotin-dependent acyl-CoA carboxylase complex is composed of AccA1, which contains the biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) domains, and AccD1, which contains the carboxyl transferase (CT) domain (PubMed:25695631). The AccA1/AccD1 complex forms a dodecamer (PubMed:25695631). - Fatty acid biosynthesis in Mycobacterium tuberculosis: lateral gene transfer, adaptive evolution, and gene duplication
Kinsella, Proceedings of the National Academy of Sciences of the United States of America 2003 - “...genes in the CDC1551 genome that perform this enzymatic function, MT2576 and MT3384. A diagram of this tree is too large to include here and is published as...”
- Comparative Proteomic Analysis of Cell Wall Proteins of Aminoglycosides Resistant and Sensitive Mycobacterium tuberculosis Clinical Isolates
Sharma, Current protein & peptide science 2024 (PubMed)- “...Rv1308 belonged to intermediary metabolism and respiration; Rv2115c to the cell wall and cell processes; Rv2501c, Rv2247 and Rv0295c to lipid metabolism; and Rv2416c to virulence, detoxification/adaptation. Notably, variations in these proteins support cell wall integrity, aiding mycobacteria's establishment and proliferation. Molecular docking study revealed that...”
- Polyphosphate kinase-1 regulates bacterial and host metabolic pathways involved in pathogenesis of Mycobacterium tuberculosis
Chugh, Proceedings of the National Academy of Sciences of the United States of America 2024 - “...identified polyP 700 interacting proteins belonged to the ACC family of enzymes. These included AccA1 (Rv2501c), AccD1 (Rv2502c), AccA2 (Rv0973c), AccD2 (Rv0974c), AccD4 (Rv3799c), AccD5 (Rv3280), and AccE5 (Rv3281) ( SI Appendix , Table S1 and Dataset S1 ). Since polyP has been demonstrated to replace...”
- Intrabacterial lipid inclusion-associated proteins: a core machinery conserved from saprophyte Actinobacteria to the human pathogen Mycobacterium tuberculosis
Dargham, FEBS open bio 2023 - “...MMAR_1153 MUL_0918 ML0862 RopRjos Rv0400c fadE7 AcylCoA dehydrogenase Nonessential MSMEG_2466 MAB_0822 MMAR_0698 MUL_2825 ML0737 RopRjos Rv2501c accA1 Acetyl/propionylcoenzyme A carboxylase alpha chain Nonessential MSMEG_4716 MAB_4539c MMAR_3848 MUL_3779 ML0726c RopRjos Rv0824c desA1 AcylACP desaturase Essential MSMEG_5773 MAB_0754c MMAR_4856 MUL_0445 ML2185 RopRjos Rv3130c tgs1 Triacylglycerol synthase Nonessential MSMEG_5242...”
- “...(FadB2), Rv3229c (DesA3), Rv0154c (FadE2), Rv2244 (AcpM), Rv3800c (Pks13), Rv2524c (Fas), Rv0400c (FadE7), Rv0824c (DesA1), Rv2501c (AccA1), and Rv2247 (AccD6). Three additional proteins, Rv1544, Rv3720, and Rv0437c, annotated as putative ketoacyl reductase, fattyacylphospholipid synthase, and phosphatidylserine decarboxylase, respectively, were also identified. A simplified representation of the...”
- Mycobacteriophage SWU1 gp39 can potentiate multiple antibiotics against Mycobacterium via altering the cell wall permeability
Li, Scientific reports 2016 - “...fatty acid metabolism MSMEG_6041 2.41 Rv3573c lipid degradation MSMEG_3465 2.18 Rv1925 lipid degradation MSMEG_4716 2.51 Rv2501c lipid metabolism MSMEG_5907 2.15 Rv3505 involved in lipid degradation MSMEG_5923 2.10 Rv3523 lipid carrier protein MSMEG_5922 2.18 Rv3522 lipid transfer protein...”
- Precision methylome characterization of Mycobacterium tuberculosis complex (MTBC) using PacBio single-molecule real-time (SMRT) technology
Zhu, Nucleic acids research 2016 - “...assembly protein SufB -/- -/- +/+ -/+ +/+ -/- NA NA -/- -/- +/+ -/- Rv2501c No-data acetyl-/propionyl-CoA carboxylase subunit alpha +/+ +/+ -/+ -/+ +/+ -/+ NA NA -/- -/- -/- -/+ Rv1917c Non-essential PPE family protein, PPE34 -/+ -/+ +/+ +/+ +/+ +/+ NA...”
- Characterization of the mycobacterial acyl-CoA carboxylase holo complexes reveals their functional expansion into amino acid catabolism
Ehebauer, PLoS pathogens 2015 - “...at least four different mycobacterial YCC holo complexes. From these identified assemblies, the AccD1 (Rv2502c)-AccA1 (Rv2501c) YCC complex was selected for functional and structural studies. We demonstrate that this complex encodes a 3-methylcrotonyl-CoA carboxylase (MCC) involved in leucine catabolism. Electron micrographs of the AccD1-AccA1 holo complex...”
- “...Remarkably, in addition to the conserved operon organization, the expression of most genes (Rv2495c to Rv2501c) of the citE-scoA operon and the pdhABC operon are strongly correlated (for further details see Operon Correlation Browser ( www.broadinstitute.org/annotation/tbdb/operon/ ), suggesting that the effects observed are coupled. This observation...”
- bkaR is a TetR-type repressor that controls an operon associated with branched-chain keto-acid metabolism in Mycobacteria
Balhana, FEMS microbiology letters 2013 - “...MSMEG_4717 9.0 * 3.7E-05 accD1 Rv2502c Acetyl/propionyl-coenzyme A carboxylase ( subunit) MSMEG_4716 15.0 1.5E-05 accA1 Rv2501c Acetyl/propionyl-coenzyme A carboxylase ( subunit) MSMEG_4715 13.7 5.8E-06 fadE19 Rv2500c Acyl-CoA dehydrogenase MSMEG_4714 9.5 1.1E-04 Rv2499c Rv2499c Hydratase MSMEG_4713 16.5 4.6E-06 citE Rv2498c HpcH/HpaI aldolase/citrate lyase family protein MSMEG_4712 8.4...”
- A chemical proteomics approach to profiling the ATP-binding proteome of Mycobacterium tuberculosis
Wolfe, Molecular & cellular proteomics : MCP 2013 - “...3 3.50 Aldehyde dehydrogenase Rv0458 55 kDa (0.0066) 19 2 3.25 Acetyl-/propionyl-CoA carboxylase subunit, AccA1 Rv2501c 71 kDa (0.0001) 45 5 3.17 Fatty-acid-CoA ligase, FadD7 Rv0119 55 kDa (0.0020) 27 3 3.17 Electron transfer flavoprotein subunit, FixA Rv3029c 28 kDa (0.0020) 27 3 3.17 Fatty-acid-CoA ligase,...”
- “...NSAF_ATPS a Avg. NSAF_ATP b Fold change Competitive Binders of Desthiobiotin-ATP Acetyl-/propionyl-CoA carboxylase subunit, AccA1 Rv2501c 71 kDa (0.0000) 1.00 15.67 15.67 Glutamyl-tRNA amidotransferase subunit B, GatB Rv3009c 55 kDa (0.0000) 2.33 28.33 12.14 Inosine-5-monophosphate dehydrogenase, GuaB3 Rv3410c 39 kDa (0.0000) 1.00 11.67 11.67 Transmembrane serine/threonine-protein...”
- More
Q2RYD8 propionyl-CoA carboxylase from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1)
Rru_A0052 Biotin carboxylase from Rhodospirillum rubrum ATCC 11170
50% identity, 66% coverage
- Study of the Production of Poly(Hydroxybutyrate-co-Hydroxyhexanoate) and Poly(Hydroxybutyrate-co-Hydroxyvalerate-co-Hydroxyhexanoate) in Rhodospirillum rubrum
Cabecas, Applied and environmental microbiology 2022 (secret) - Genetic Plasticity and Ethylmalonyl Coenzyme A Pathway during Acetate Assimilation in Rhodospirillum rubrum S1H under Photoheterotrophic Conditions
De, Applied and environmental microbiology 2018 - “...5 6 3 Q2RPT8 Q2RPT6 Q2RV43 Q2RXX3 Q2RV44 Q2RYD8 Q2RYD7 Q2RRG6 Rru_A3062 Rru_A3064 Rru_A1201 Rru_A0217 Rru_A1200 Rru_A0052 Rru_A0053 Rru_A2479 1.4e3 3.4e4 2.1e4...”
- The metabolic pathways of carbon assimilation and polyhydroxyalkanoate production by Rhodospirillum rubrum in response to different atmospheric fermentation
Tang, PloS one 2024 - “...1.11E-11 2.518 Calvin-Benson-Bassham cycle Rru_A2400 Ribulose bisphosphate carboxylase 0.015 1.137 ND ND methylmalonyl-CoA (MMC) pathway Rru_A0052 Biotin carboxylase ND ND 1.18E-90 2.350 Rru_A0053 Carboxyl transferase ND ND 2.41E-83 2.305 Rru_A2318 2-methylcitrate dehydratase 1.72E-49 5.708 4.62E-28 3.536 Rru_A2320 Phosphoenolpyruvate phosphomutase 6.41E-28 4.305 6.84E-11 1.857 Rru_A2319 2-methylcitrate synthase/Citrate...”
- Effects of Mixing Volatile Fatty Acids as Carbon Sources on Rhodospirillum rubrum Carbon Metabolism and Redox Balance Mechanisms
Cabecas, Microorganisms 2021 - “...Figure 3 ), confirming the previous reports [ 18 ]. This pathway requires five enzymes (Rru_A0052, Rru_A0053, Rru_A1572, Rru_A2480, Rru_A1927), among which three were significantly more abundant in propionate conditions than in butyrate conditions: the biotin carboxylase (Rru_A0052, fold change: 0.63), the methylmalonyl-CoA mutase (Rru_A2480, fold...”
- “...Change (Mix Butyrate/Mix Propionate) ** P-Value No. of Identified Peptides *** Description Methylmalonyl-CoA pathway tr|Q2RYD8 Rru_A0052 0.63 3.41 10 2 1.81 9.50 10 3 5 Biotin carboxylase tr|Q2RYD7 Rru_A0053 0.83 8.70 10 2 1.81 4.95 10 2 6 Carboxyl transferase tr|Q2RU23 Rru_A1572 1.30 3.49 10 1...”
- Photoheterotrophic Assimilation of Valerate and Associated Polyhydroxyalkanoate Production by Rhodospirillum rubrum
Bayon-Vicente, Applied and environmental microbiology 2020 (secret) - New perspectives on butyrate assimilation in Rhodospirillum rubrum S1H under photoheterotrophic conditions
De, BMC microbiology 2020 - “...Rru_A1200 Malyl-CoA thioesterase 1.3 2e-02 6 0.8 2e-03 1.0 8e-01 1.1 9e-02 1.1 3e-02 6 Rru_A0052 Biotin carboxylase 1.2 7e-02 6 0.8 3e-05 0.9 4e-02 1.0 1e-01 1.1 1e-02 6 Rru_A0053 Carboxyl transferase 1.2 8e-03 6 0.8 8e-05 0.9 2e-02 1.0 1e-01 1.1 9e-03 6 Rru_A2479...”
- “...3.42 2e-07 Rru_A0217 L-Malyl-CoA/b-methylmalyl-CoA lyase 0.05 3.11 1e-05 Rru_A1200 b Malyl-CoA thioesterase n.a. n.a. n.a. Rru_A0052 Biotin carboxylase 0.03 1.83 2e-05 Rru_A0053 Carboxyl transferase 0.09 1.77 2e-04 Rru_A2479 Methylmalonyl-CoA mutase 0.12 0.53 1e-02 Rru_A1927 Acetyl-CoA hydrolase 0.01 0.1 3e-01 Rru_A2129 Fumarate hydratase class II 0.15 0.03...”
- Genetic Plasticity and Ethylmalonyl Coenzyme A Pathway during Acetate Assimilation in Rhodospirillum rubrum S1H under Photoheterotrophic Conditions
De, Applied and environmental microbiology 2018 - “...Rru_A3062 Rru_A3064 Rru_A1201 Rru_A0217 Rru_A1200b Rru_A0052 Rru_A0053 Rru_A2479 Locus Ethylmalonyl-CoA pathway Rru_A1927 Rru_A2964 Rru_A3063 Rru_A1572a...”
- “...Q2RYD7 Q2RRG6 Rru_A3062 Rru_A3064 Rru_A1201 Rru_A0217 Rru_A1200 Rru_A0052 Rru_A0053 Rru_A2479 1.4e3 3.4e4 2.1e4 3.3e4 5.1e4 1.2e6 4.4e6 3.4e1 7.89 6.37 1.34...”
SCO2777 acetyl/propionyl CoA carboxylase alpha subunit from Streptomyces coelicolor A3(2)
54% identity, 68% coverage
- Origin of the 3-methylglutaryl moiety in caprazamycin biosynthesis
Bär, Microbial cell factories 2022 - “...M1154 revealed genes homologue to liuA ( sco2779 ), liuB ( sco2776 ), liuD ( sco2777 ) and liuE ( sco2778 ) (Additional file 1 : Fig. S6). No homologue was found for liuC in this cluster though, raising the question if this gene is located...”
- “...is hijacked for caprazamycin biosynthesis, we next generated mutants with single deletions of sco2776 , sco2777 , sco2778 or sco2779 resulting in S. coelicolor M1154 sco2776, S. coelicolor M1154 sco2777, S. coelicolor M1154 sco2778 and S. coelicolor M1154 sco2779 . Successful deletion and absence of wildtype...”
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances in S. lividans and S. coelicolor Suggests a Link between Various Stresses and Antibiotic Production
Clara, International journal of molecular sciences 2022 - “...the biosynthesis of malonyl or methyl-malonylCoA, possibly used for fatty acids and/or polyketide (ACT) biosynthesis. SCO2777 and SCO2776 were highly abundant in SC but poorly abundant in SL , in both Pi conditions (Figure S2A of [ 9 ]), suggesting that SCO2775 represses the expression of...”
- AccR, a TetR Family Transcriptional Repressor, Coordinates Short-Chain Acyl Coenzyme A Homeostasis in Streptomyces avermitilis
Lyu, Applied and environmental microbiology 2020 (secret) - Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus
Lee, The ISME journal 2020 - “...acetyl-CoA; (ii) the acetyl-CoA synthetase (SCO6195) converted acetate to acetyl-CoA; and (iii) acetyl-CoA carboxylases (SCO2776, SCO2777, and SCO4921) converted acetyl-CoA to malonyl-CoA (Fig. 1e ). Considering that the transcription levels of genes involved in other acetyl-CoA-associated pathways, such as glycolysis and the citrate cycle, showed no...”
- A Novel Two-Component System, Encoded by the sco5282/sco5283 Genes, Affects Streptomyces coelicolor Morphology in Liquid Culture
Arroyo-Pérez, Frontiers in microbiology 2019 - “...degradation I 8.90E-09 1.41E-04 4.44E-16 SCO2776 Carboxylase 2.11 Amino acid degradation I 3.14E-15 1.33E-01 3.77E-15 SCO2777 Carboxylase 2.12 Amino acid degradation I 1.85E-15 1.16E-01 0.00E+00 SCO2778 Hydroxymethylglutaryl-CoA lyase 1.96 Amino acid degradation I 2.54E-13 2.75E-01 1.06E-03 SCO2779 Acyl-CoA dehydrogenase 2.08 Amino acid degradation I 6.17E-15 1.50E-01...”
Q1H158 Biotin carboxylase from Methylobacillus flagellatus (strain ATCC 51484 / DSM 6875 / VKM B-1610 / KT)
47% identity, 94% coverage
- Generating interacting protein sequences using domain-to-domain translation.
Meynard-Piganeau, Bioinformatics (Oxford, England) 2023 - “...using Alphafold ( Jumper et al. 2021 ). To this end, we selected the protein Q1H158 from the validation set, which contains the Pfam domains PF00289 and PF02785. We then replaced the domain PF00289 with homologous sequences from the validation set, keeping the rest of the...”
- “...where single domains have been replaced with homologous natural sequences. The results are based on Q1H158, which contains domains PF00289 and PF02785, which are in contact in PDB 5ks8. Homologous PF00289 sequences are sampled from the validation set and inserted into the Q1H158 sequence, measuring the...”
C5O77_01100 acetyl-CoA carboxylase biotin carboxylase subunit from Limosilactobacillus reuteri
48% identity, 96% coverage
A4APF1 biotin carboxylase (EC 6.3.4.14) from Maribacter sp. HTCC2170 (see paper)
47% identity, 97% coverage
jk1550 acyl-CoA carboxylase, alpha subunit from Corynebacterium jeikeium K411
47% identity, 64% coverage
Lreu_0984 acetyl-CoA carboxylase biotin carboxylase subunit from Limosilactobacillus reuteri subsp. reuteri
Lreu_0984 acetyl-CoA carboxylase, biotin carboxylase from Lactobacillus reuteri DSM 20016
47% identity, 96% coverage
PA14_71740 putative biotin carboxylase subunit of a transcarboxylase from Pseudomonas aeruginosa UCBPP-PA14
NP_254123 acetyl-CoA carboxylase subunit alpha from Pseudomonas aeruginosa PAO1
Q9HTD0 Biotin carboxylase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA5436 acetyl-CoA carboxylase from Pseudomonas aeruginosa PAO1
50% identity, 94% coverage
- High-resolution in situ transcriptomics of Pseudomonas aeruginosa unveils genotype independent patho-phenotypes in cystic fibrosis lungs
Rossi, Nature communications 2018 - “...encoding for the sugar transporter gltBFK-oprB and for enzymes involved in pyruvate degradation to oxaloacetate (PA14_71740) and acetyl-CoA production from pyruvate ( aceEF ) (Fig. 4 and Supplementary Data 2 ). Similarly, expression of genes coding for enzymes of the lower part of the TCA cycle...”
- Identification of pyruvate carboxylase genes in Pseudomonas aeruginosa PAO1 and development of a P. aeruginosa-based overexpression system for alpha4- and alpha4beta4-type pyruvate carboxylases.
Lai, Applied and environmental microbiology 2006 - GeneRIF: The alpha4beta4 PYC (pyruvate carboxylase) of this organism was encoded by the ORFs PA5436 and PA5435.
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa
Dulyayangkul, Frontiers in microbiology 2020 - “...<0.05 0.019 0.19 0.049 Q51567 SuccinateCoA ligase [ADP-forming] subunit alpha SucD <0.05 0.002 <0.05 0.002 Q9HTD0 Probable biotin carboxylase subunit of a transcarboxylase PA5436 <0.05 0.027 <0.05 0.027 Q9HVI7 Serine hydroxymethyltransferase 3 GlyA2 <0.05 0.000 0.15 0.002 Q9HVK2 Uncharacterized protein PA4582 >20 0.002 >20 0.004 Q9HX11...”
- Distinct transcriptome and traits of freshly dispersed <i>Pseudomonas aeruginosa</i> cells
Kalia, mSphere 2024 - “...0.87 0.305 PA5016 Dihydrolipoamide acetyltransferase 0.346 0.644 0.117 PA5435 Probable transcarboxylase subunit 0.088 0.21 1.44 PA5436 Probable biotin carboxylase subunit of a transcarboxylase 0.42 0.46 1.58 Pentose phosphate pathway PA0330 Ribose 5-phosphate isomerase 0.77 0.28 0.302 PA0548 Transketolase 0.96 0.432 0.152 PA0555 Fructose-1,6-bisphosphate aldolase 0.72 0.389...”
- Systems-wide dissection of organic acid assimilation in Pseudomonas aeruginosa reveals a novel path to underground metabolism
Dolan, 2022 - Systems-Wide Dissection of Organic Acid Assimilation in Pseudomonas aeruginosa Reveals a Novel Path To Underground Metabolism
Dolan, mBio 2022 - “...FC) during growth on propionate. By contrast, the pyruvate carboxylase-encoding genes pycA (PA5435) and pycB (PA5436), which catalyze the ATP-dependent carboxylation of pyruvate to yield oxaloacetate (OAA), were upregulated (2.6 FC) as was the regulator PycR ( 21 , 22 ). These alterations matched the corresponding...”
- In Vitro Activity of the Ultrabroad-Spectrum Beta-Lactamase Inhibitor QPX7728 in Combination with Multiple Beta-Lactam Antibiotics against Pseudomonas aeruginosa
Lomovskaya, Antimicrobial agents and chemotherapy 2021 - “...IMP-6 >64 64 16 >64 >64 PAM3267 NF Up VIM-5 32 16 32 64 8 PA5436 NF Up VEB-9 16 32 32 32 8 PA5388 NF Up VIM-2; 32 64 32 16 8 PA5282 NF Up CTX-M-3 16 32 64 32 8 PA5376 FL Wt NDM-1...”
- Over-Expression of Hypochlorite Inducible Major Facilitator Superfamily (MFS) Pumps Reduces Antimicrobial Drug Susceptibility by Increasing the Production of MexXY Mediated by ArmZ in Pseudomonas aeruginosa
Dulyayangkul, Frontiers in microbiology 2020 - “...subunit alpha SucD <0.05 0.002 <0.05 0.002 Q9HTD0 Probable biotin carboxylase subunit of a transcarboxylase PA5436 <0.05 0.027 <0.05 0.027 Q9HVI7 Serine hydroxymethyltransferase 3 GlyA2 <0.05 0.000 0.15 0.002 Q9HVK2 Uncharacterized protein PA4582 >20 0.002 >20 0.004 Q9HX11 Uncharacterized protein PA4016 0.19 0.002 <0.05 0.000 Q9HXR2...”
- Traditional Chinese Medicine Tanreqing Inhibits Quorum Sensing Systems in Pseudomonas aeruginosa
Yang, Frontiers in microbiology 2020 - “...PA4100 1.3 Probable dehydrogenase PA4956 rhdA 1.3 Thiosulfate:cyanide sulfurtransferase PA5435 2.3 1.3 Probable transcarboxylase subunit PA5436 2.7 1.1 Probable biotin carboxylase subunit of a transcarboxylase PA4442 cysN 1.7 1.9 + ATP sulfurylase GTP-binding subunit/APS kinase PA4443 cysD 2.1 1.1 + ATP sulfurylase small subunit PA3181 1.6...”
- The development of a new parameter for tracking post-transcriptional regulation allows the detailed map of the Pseudomonas aeruginosa Crc regulon
Corona, Scientific reports 2018 - “...ammonia-lyase 0,05 0,6 1,76 CCM pycB PA5435 Probable transcarboxylase subunit 1,69 1,05 1,29 CCM pycA PA5436 Probable biotin carboxylase subunit of a transcarboxylase 1,09 0,88 1,4 Transport PA5504 D-methionine ABC transporter membrane protein 0,19 0,29 1,01 Catabolism pauA7 PA5508 Glutamylpolyamine synthetase homologue 4,03 0,8 6,32 Transport...”
- Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures
Chan, PeerJ 2016 - “...12.316 26.3594 1.09778 2.00644 0.00085 0.00810873 gene4882 lldP 4.00184 8.55432 1.09599 1.60525 0.00815 0.0408179 gene5562 PA5436 7.08683 14.5432 1.03713 1.71082 0.00505 0.0294665 gene3027 rne 24.3393 49.8051 1.03301 2.10105 0.00025 0.00268304 gene3884 iscS 24.603 48.5117 0.9795 1.92781 0.00175 0.0136591 gene4784 hitA 10.4753 20.2269 0.949286 1.53913 0.00885 0.0435971...”
- More
B739_0889 acetyl-CoA carboxylase biotin carboxylase subunit from Riemerella anatipestifer RA-CH-1
48% identity, 97% coverage
- Investigation of TbfA in Riemerella anatipestifer using plasmid-based methods for gene over-expression and knockdown
Liu, Scientific reports 2016 - “...promoters of the genes B739_0921 (rpkm:27503, high transcriptional activity), B739_0973 (rpkm:2236, medium transcriptional activity) and B739_0889 (rpkm:993, low transcriptional activity) from RA-CH-1 and used them to replace the ermF promoter in plasmid pLMF01, giving the plasmids pLMF03, pLMF04 and pLMF05, respectively ( Fig. S1 ). To...”
- “...pLMF04, which contained the P B739_0973 promoter region. For plasmid pLMF05, which contained the P B739_0889 promoter region, there was lower expression than with plasmids pLMF04 and pLMF03 ( Fig. S3 ). To further ensure the plasmids can be used for complementation, we also used R....”
N646_4047 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Vibrio alginolyticus NBRC 15630 = ATCC 17749
46% identity, 65% coverage
BAB1_0018 Biotin/lipoyl attachment:Biotin-requiring enzyme, attachment site:Carbamoyl-phosphate synthase L chain, ATP-binding:Carbamoyl... from Brucella melitensis biovar Abortus 2308
50% identity, 67% coverage
- ClpP protease modulates bacterial growth, stress response, and bacterial virulence in Brucella abortus
Sun, Veterinary research 2023 - “...UbiG, BAB1_0241, SucC, BAB1_1964, and Bpt, while the top 10 down-regulated proteins were ClpS, BAB1_0115, BAB1_0018, BAB1_0296, BAB1_0875, BAB1_1435, BAB1_1357, BAB2_0019, BAB1_1214, and BAB2_0547. Integrated analysis revealed the global roles of ClpP in B. abortus 2308 According to the proteomic and transcriptomic profiles, clpP deletion extensively...”
AS87_RS06995 acetyl-CoA carboxylase biotin carboxylase subunit from Riemerella anatipestifer Yb2
48% identity, 97% coverage
BLJAPNOD_06206 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Ensifer sp. M14
47% identity, 67% coverage
atuF / Q9HZV6 cis-geranyl-CoA carboxylase α-subunit (EC 6.4.1.5) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
PA2891 probable biotin carboxylase/biotin carboxyl carrier protein from Pseudomonas aeruginosa PAO1
51% identity, 67% coverage
- The opportunistic pathogen Pseudomonas aeruginosa exploits bacterial biotin synthesis pathway to benefit its infectivity
Shi, PLoS pathogens 2023 - “.... Genome-wide mining of P . aeruginosa reveals five putative biotinylated enzymes, namely PA1400, PA2012, PA2891, PA4847, and PA5435 ( S1 Table ). D. Streptavidin blot analyses for crude extracts of E . coli and P . aeruginosa . Unlike E . coli displaying a well-known...”
- “...of multiple biotinylated proteins. E. SDS-PAGE (12%) profile for three biotinylated enzymes (PA1400, PA2012, and PA2891). Three of five predictive biotinylated enzymes were overexpressed, purified, and judged with SDS-PAGE (12%). F. Western blot analysis of the three recombinant proteins (PA1400, PA2012, and PA2891) using anti-6x His...”
- The Pseudomonas aeruginosa Isohexenyl Glutaconyl Coenzyme A Hydratase (AtuE) Is Upregulated in Citronellate-Grown Cells and Belongs to the Crotonase Family
Poudel, Applied and environmental microbiology 2015 - “...PA2014 PA2015 PA2886 PA2887 PA2888 PA2889 PA2890 PA2891 PA2892 PA4330 Gene Structure-Function of Pseudomonas aeruginosa AtuE corresponding to rat ECH catalytic...”
- Swarming of Pseudomonas aeruginosa is controlled by a broad spectrum of transcriptional regulators, including MetR
Yeung, Journal of bacteriology 2009 - “...revealed PA1843 (metH), PA2399 (pvdD), PA2413 (pvdH), PA2891, PA4144, and PA4223. Conversely, PA0633, PA0848, and PA5125 (ntrC) were negatively regulated by...”
- The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa
Aguilar, Applied and environmental microbiology 2006 - “...PAO1::ISlacZ/hah (ID 10788), transposon insertion in ORF PA2891 PAO1::ISlacZ/hah (ID 8377), transposon insertion in ORF PA2893 PAO1::ISlacZ/hah (ID 11254),...”
- “...PAO1SM mutant obtained by Himar1::Gmr transposition in ORF PA2891, unable to grow on citronellol PAO1SM mutant obtained by Himar1::Gmr transposition in ORF...”
- Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa
Höschle, Microbiology (Reading, England) 2005 (PubMed)- “...at 37 uC. Insertion mutagenesis. Disruption of atuF (PA2891) in P. aerugi- nosa was carried out using pKnockout-G for rapid gene inactivation (Windgassen et...”
- “...gene products of liuD (PA2012; 74 kDa) and atuF (PA2891; 71 kDa), respectively (Figs 2 and 3). The three non-biotin-containing polypeptides (band nos 4/5 and 6,...”
- The gnyRDBHAL cluster is involved in acyclic isoprenoid degradation in Pseudomonas aeruginosa
Díaz-Pérez, Applied and environmental microbiology 2004 - “...the GnyA protein (left). crotonoyl-CoA carboxylase, ORF PA2891) biotinylated subunits of their respective carboxylases. In agreement with data from the RT-PCR...”
BMEI1925 acetyl-CoA carboxylase alpha chain / propionyl-CoA carboxylase alpha chain from Brucella melitensis 16M
50% identity, 67% coverage
LSA0820 Acetyl-CoA carboxylase (biotin carbooxylase subunit) from Lactobacillus sakei subsp. sakei 23K
48% identity, 97% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...LSA0817 fabF 3-oxoacyl-[acyl carrier protein] synthetase II -0.7 LSA0819 fabZ (3R)-hydroxymyristoyl-[acyl carrier proetin] dehydratase 0.7 LSA0820 accC Acetyl-CoA carboxylase (biotin carbooxylase subunit) -0.7 LSA0821 accD Acetyl-CoA carboxylase (carboxyl transferase beta subunit) 0.8 LSA0822 accA Acetyl-CoA carboxylase (carboxyl transferase alpha subunit) 0.6 LSA0823 fabI Enoyl [acyl carrier...”
accC / Q877I5 acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) from Acidianus brierleyi (see 2 papers)
Q877I5 biotin carboxylase (EC 6.3.4.14); acetyl-CoA carboxylase (subunit 2/3) (EC 6.4.1.2); propionyl-CoA carboxylase (subunit 1/3) (EC 6.4.1.3) from Acidianus brierleyi (see 2 papers)
accC / BAC55867.1 biotin carboxylase subunit of acetyl-CoA carboxylase from Acidianus brierleyi (see paper)
45% identity, 86% coverage
BSU18240 acetyl-CoA carboxylase biotin carboxylase subunit from Bacillus subtilis subsp. subtilis str. 168
49% identity, 92% coverage
- The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis
Eichenberger, PLoS biology 2004 - “...ykvI (BSU13710), ykvU (BSU13830), ylbJ (BSU15030), ylbO/gerR (BSU15090), yngE (BSU18210), yngF (BSU18220), yngG (BSU18230), yngH (BSU18240), yngI (BSU18250), yngJ (BSU18260), yoaB (BSU18540), yoaD (BSU18560), yodU (BSU19810), ypqA (BSU22240), ypqP (BSU21670), yqfT (BSU25120), yqhV (BSU24440), yrdK (BSU26680), yydB (BSU40220), yydC (BSU40210), yydD (BSU40200), yydG (BSU40170), yydH (BSU40160),...”
Q2FXX1 Acetyl-CoA carboxylase, biotin carboxylase, putative from Staphylococcus aureus (strain NCTC 8325 / PS 47)
SA1434 hypothetical protein from Staphylococcus aureus subsp. aureus N315
SAV1606 acetyl-CoA carboxylase from Staphylococcus aureus subsp. aureus Mu50
SAOUHSC_01709 acetyl-CoA carboxylase, biotin carboxylase, putative from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_1563 acetyl-CoA carboxylase, biotin carboxylase from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL1661 acetyl-CoA carboxylase, biotin carboxylase, putative from Staphylococcus aureus subsp. aureus COL
B4602_RS08180, KMZ21_07575, SAV_RS08635, USA300HOU_RS08555 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Staphylococcus aureus subsp. aureus USA300_TCH1516
50% identity, 91% coverage
- Antibacterial Activity of Juglone against Staphylococcus aureus: From Apparent to Proteomic
Wang, International journal of molecular sciences 2016 - “...2 16.40 0.650 0.003 Q2G273 urease accessory protein ureg ureG 3 3 22.30 0.796 0.021 Q2FXX1 acetyl- biotin carboxylase subunit SAOUHSC_01709 4 4 50.20 0.761 0.022 Q2G1X5 queuosine biosynthesis protein SAOUHSC_00720 3 3 16.00 0.831 0.000 Q2FXT6 queuine tRNA-ribosyltransferase tgt 2 2 43.30 0.603 0.008 Protein...”
- “...Thioredoxin trxA 9 9 11.40 0.654 0.001 Q2FXI6 Thioredoxin SAOUHSC_01860 8 8 11.80 0.781 0.003 Q2FXX1 acetyl- biotin carboxylase subunit SAOUHSC_01709 4 4 50.20 0.761 0.022 Others Q2FVB3 antibiotic transport system permease SAOUHSC_02821 1 1 28.90 1.299 0.007 Q2FWL8 transcriptional regulator SAOUHSC_02271 2 2 8.20 1.448...”
- Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism
Bohn, Nucleic acids research 2010 - “...) 0.32 0.36 Aminomethyltransferase SA1432 0.43 0.46 18.04 Hypothetical Mn 2+ and Fe 2+ transporter SA1434 0.43 AcetylCoA carboxylase SA1435 0.45 AcetylCoA carboxylase SA1517 ( citC ) 0.40 Isocitrate dehydrogenase SA1518 ( citZ ) 0.37 0.48 14.80 Methylcitrate synthase SA1553 ( fhs ) 0.30 f 14.08...”
- Direct targets of CodY in Staphylococcus aureus
Majerczyk, Journal of bacteriology 2010 - “...of UAMS-1 Expression ratio Gene group and annotationa SA1433 SA1434 SA1435 SA0012 BCAAs SA1477 thrA hom thrC thrB thrA SA1361, hom, thrCB, SA1365 lysC asd dapA...”
- Characterization of the Staphylococcus aureus heat shock, cold shock, stringent, and SOS responses and their effects on log-phase mRNA turnover
Anderson, Journal of bacteriology 2006 - “...SA0502 SA0503 SA0595 SA1058 SA1360 SA1433 SA1434 SA1594 SA1787 2.4 7.4 2.3* Carbohydrates sa_c9528s8308_a_at sa_c8477s7437_at sa_c629s448_at sa_c2791s2362_a_at...”
- Metabolic activity, urease production, antibiotic resistance and virulence in dual species biofilms of Staphylococcus epidermidis and Staphylococcus aureus
Vandecandelaere, PloS one 2017 - “...subunit A -3.25 + 0.20 0.00 Metabolism lctE SAV_RS01365 L-lactate dehydrogenase -2.16 + 0.20 0.00 SAV1606 SAV_RS08635 Acetyl-CoA carboxylase -2.17 + 0.01 0.00 lacD SAV_RS11990 Tagatose 1,6-diphosphate aldolase -2.21 + 0.19 lacC SAV_RS11995 Tagatose-6-phosphate kinase -2.41 + 0.20 0.00 lacB SAV_RS12000 Galactose-6-phosphate isomerase subunit LacB -2.80...”
- Antibacterial Activity of Juglone against Staphylococcus aureus: From Apparent to Proteomic
Wang, International journal of molecular sciences 2016 - “...urease accessory protein ureg ureG 3 3 22.30 0.796 0.021 Q2FXX1 acetyl- biotin carboxylase subunit SAOUHSC_01709 4 4 50.20 0.761 0.022 Q2G1X5 queuosine biosynthesis protein SAOUHSC_00720 3 3 16.00 0.831 0.000 Q2FXT6 queuine tRNA-ribosyltransferase tgt 2 2 43.30 0.603 0.008 Protein synthesis Q2FW17 50s ribosomal protein...”
- “...0.654 0.001 Q2FXI6 Thioredoxin SAOUHSC_01860 8 8 11.80 0.781 0.003 Q2FXX1 acetyl- biotin carboxylase subunit SAOUHSC_01709 4 4 50.20 0.761 0.022 Others Q2FVB3 antibiotic transport system permease SAOUHSC_02821 1 1 28.90 1.299 0.007 Q2FWL8 transcriptional regulator SAOUHSC_02271 2 2 8.20 1.448 0.001 Q2G2L6 pf09954 family protein...”
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Genetic Factors Involved in Human Endothelial Cells Damage, an Important Phenotype Correlated with Persistent Endovascular Infection
Xiao, Antibiotics (Basel, Switzerland) 2022 - “...hypothetical branched-chain alpha-keto acid dehydrogenase subunit E2 16.83 18.68 SAUSA300_0419 hypothetical tandem lipoprotein 16.78 3.58 SAUSA300_1563 accC acetyl-CoA carboxylase, biotin carboxylase 16.73 11.04 SAUSA300_2027 alr alanine racemase 16.70 16.05 SAUSA300_2607 hisA phoribosyl)-5-((5-phosphoribosylamino)methylideneamino) imidazole-4-carboxamide 16.70 11.46 SAUSA300_0023 hypothetical hypothetical protein 16.69 16.09 SAUSA300_1622 tig trigger factor 16.44...”
- “...8.43 13.43 a SAUSA300_1696 dat 12.74 5.48 14.99 1.34 SAUSA300_0974 purN 11.07 8.08 20.58 a SAUSA300_1563 accC 16.73 11.04 11.82 0.72 SAUSA300_0041 hypothetical 10.41 2.09 3.30 a SAUSA300_0994 pdhB 11.20 8.12 19.36 a SAUSA300_0186 argC 12.92 16.00 15.20 2.13 SAUSA300_1992 agrA 5.34 14.81 3.82 1.77 SAUSA300_0355...”
- A new oxidative sensing and regulation pathway mediated by the MgrA homologue SarZ in Staphylococcus aureus
Chen, Molecular microbiology 2009 - “...metabolisms ( argGH, ilvD, lysC, hisC ), genes for fatty acid synthesis ( fabG , SACOL1661, SACOL0212), and genes for sugar metabolism ( gntRK, lacD, malA, treC ) show significant changes in the sarZ mutant. In particular, pflA (pyruvate formate lyase activating enzyme, Buis and Broderick...”
- “...protein SACOL0882 2.9 ABC transporter SACOL0884 2.1 -2.3 ABC transporter SACOL1476 13.8 Amino acid permiase SACOL1661 3.8 - Acetyl-CoA carboxylase, accC homologue SACOL1847 3.8 Conserved hypothetical protein SACOL1849 2.3 - Conserved hypothetical protein SACOL1915 4.1 Glutamate ABC transporter SACOL1916 3.8 Amino acid ABC transporter SACOL2461 3.7...”
- Combined proteomic and transcriptomic analysis of the antimicrobial mechanism of tannic acid against Staphylococcus aureus
Wang, Frontiers in pharmacology 2023 - “...WP_000215388.1 WecB/TagA/CpsF family glycosyltransferase 0.21 0.33 B4602_RS03245 - WP_001241179.1 Glycosyltransferase family 2 protein 0.62 0.29 B4602_RS08180 accC1 WP_000942744.1 Acetyl-CoA carboxylase biotin carboxylase subunit 0.79 1.16 B4602_RS08185 BCCP WP_001019340.1 Acetyl-CoA carboxylase biotin carboxyl carrier protein subunit 1.03 0.88 B4602_RS05185 purK WP_001010407.1 5-(carboxyamino)imidazole ribonucleotide synthase 0.90 2.44 B4602_RS05935...”
- Hybrid assembly using long reads resolves repeats and completes the genome sequence of a laboratory strain of Staphylococcus aureus subsp. aureus RN4220
Panthee, Heliyon 2022 - “...1384986..1413593(-) - 22 KMZ21_06985 316 DUF 1469250..1470200(-) + 23 KMZ21_07000 309 DUF 1473421..1474350(-) + 24 KMZ21_07575 453 Acetyl-CoA carboxylase biotin carboxylase subunit 1578089..1579450(-) - 25 KMZ21_08010 550 IS1182 transposase 1667723..1669374(-) - 26 KMZ21_08245 505 hypothetical protein 1721716..1723233(-) + 27 KMZ21_08370 220 transposase 1757495..1758156(-) - 28 KMZ21_08480...”
- Metabolic activity, urease production, antibiotic resistance and virulence in dual species biofilms of Staphylococcus epidermidis and Staphylococcus aureus
Vandecandelaere, PloS one 2017 - “...A -3.25 + 0.20 0.00 Metabolism lctE SAV_RS01365 L-lactate dehydrogenase -2.16 + 0.20 0.00 SAV1606 SAV_RS08635 Acetyl-CoA carboxylase -2.17 + 0.01 0.00 lacD SAV_RS11990 Tagatose 1,6-diphosphate aldolase -2.21 + 0.19 lacC SAV_RS11995 Tagatose-6-phosphate kinase -2.41 + 0.20 0.00 lacB SAV_RS12000 Galactose-6-phosphate isomerase subunit LacB -2.80 +...”
- Absence of Protoheme IX Farnesyltransferase CtaB Causes Virulence Attenuation but Enhances Pigment Production and Persister Survival in MRSA
Xu, Frontiers in microbiology 2016 - “...USA300HOU_RS02380 gltD 1.50 4.97E-02 Glutamate synthase (NADPH) small subunit USA300HOU_RS05945 murD 1.50 2.66E-02 UDP-N-acetylmuramoylalanineD-glutamate ligase USA300HOU_RS08555 1.49 2.77E-02 Biotin carboxylase USA300HOU_RS12615 hutU 1.49 3.30E-02 Urocanate hydratase USA300HOU_RS13410 1.47 3.09E-02 Possible decarboxylase USA300HOU_RS12085 rpmD 1.46 2.95E-02 Ribosomal protein L30 USA300HOU_RS11770 1.46 3.46E-02 Hypothetical membrane protein USA300HOU_RS06545 glpK...”
7ybuA / P05165 Human propionyl-coenzyme a carboxylase (see paper)
47% identity, 66% coverage
- Ligand: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (7ybuA)
PCCA_PIG / P0DTA4 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Sus scrofa (Pig) (see paper)
47% identity, 61% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:13752080). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:13752080). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then tranfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA at a much lower rate (PubMed:13752080). Other alternative minor substrates include (2E)- butenoyl-CoA/crotonoyl-CoA (PubMed:13752080).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits. Interacts (via the biotin carboxylation domain) with SIRT4. Interacts with SIRT3 and SIRT5. - Label-Free Quantitative Analysis of Pig Liver Proteome after Hepatitis E Virus Infection
Martino, Viruses 2024 - “...phosphoenolpyruvate carboxykinase, cytosolic [GTP] 18 98.33 0.0036 2.90 P26234 VCL Vinculin 18 63.18 0.0007 2.28 P0DTA4 PCCA propionyl-CoA carboxylase alpha chain, mitochondrial 18 78.68 0.0025 1.98 A0A8D1MNH9 SPTAN1 spectrin alpha chain, non-erythrocytic 1 17 65.06 0.0000 2.68 P33198 IDH2 isocitrate dehydrogenase, mitochondrial 16 66.34 0.0113 1.53...”
SAKOR_01553 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Staphylococcus aureus subsp. aureus CN1
50% identity, 91% coverage
- Protein CoAlation and antioxidant function of coenzyme A in prokaryotic cells
Tsuchiya, The Biochemical journal 2018 - “...LNTHGC*EK 98.156 SAOUHSC_02733 Membrane protein, putative 67.572 NVNVC*TIPFK 116.98 SAUSA300_1090 Pseudouridine synthase 34.634 DYTLVEC*QLETGR 88.803 SAKOR_01553 Uncharacterized protein 50.22 LIEESPC*AALTEER 106.87 pyrG Cytidine 5-triphosphate synthase 59.991 ESVIEC*R 131.12 pyrG Cytidine 5-triphosphate synthase 59.991 IALFC*DINK 153.34 pyrG Cytidine 5-triphosphate synthase 59.991 LGLYPC*SIK 86.114 yisK Fumarylacetoacetate hydrolase family...”
B2RDE0 Propionyl-CoA carboxylase alpha chain, mitochondrial from Homo sapiens
47% identity, 63% coverage
SAEMRSA15_RS08245 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Staphylococcus aureus subsp. aureus HO 5096 0412
50% identity, 91% coverage
- A functional menadione biosynthesis pathway is required for capsule production by Staphylococcus aureus
Altwiley, Microbiology (Reading, England) 2021 - “...0.00034659 SAEMRSA15_RS12900 NAD(P)-dependent oxidoreductase 0.00034659 SAEMRSA15_RS01030 type 1 glutamine amidotransferase 0.00034659 SAEMRSA15_RS00265 hypothetical protein 0.00034659 SAEMRSA15_RS08245 acetyl-CoA carboxylase biotin carboxylase subunit 0.00034659 SAEMRSA15_RS10695 LytTR family DNA-binding domain-containing protein 0.00075253 SAEMRSA15_RS10690 GHKL domain-containing protein 0.00077225 SAEMRSA15_RS02555 RNA polymerase sigma factor 0.00120048 ileS isoleucine--tRNA ligase 0.00139006 SAEMRSA15_RS12840 glycerate...”
VPA1128 putative acyl-CoA carboxylase alpha chain from Vibrio parahaemolyticus RIMD 2210633
46% identity, 63% coverage
- Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification
Liu, BMC biotechnology 2021 - “...subunit, hypothetical protein and hypothetical protein, respectively (see Additional file 4 ). Three genes VP0295, VPA1128, and VPA1735 were upregulated, while eight genes VP1092, VP1256, VP1741, nhaB, VP2351, VP2545, VP2778, and VP2826 were downregulated in sodium ion transport of biological processes. They regulated multiple proteins related...”
PP5347, PP_5347 acetyl-CoA carboxylase, biotin carboxylase from Pseudomonas putida KT2440
49% identity, 94% coverage
- Computational analysis into the potential of azo dyes as a feedstock for actinorhodin biosynthesis in Pseudomonas putida
Nayyara, PloS one 2024 - “...PP_2339, PP_2112, PP_2082, PP_1023, PP_0612, PP_3124, PP_0654, PP_2589, PP_0545, PP_2694, PP_1620, PP_0327, PP_1367, PP_1943, PP_1664, PP_5347, PP_4116, PP_1031, PP_4947 5 1 5 ALDD2y, FTHFD, ALDD2x, MDH, MICITDr, GLYOX, ACACT11, MALS, PGL, ACONTa, NACODA PP_2492, PP_0327, PP_1367, PP_1943, PP_2589, PP_0545, PP_2694, PP_0654, PP_2336, PP_4144, PP_0356, PP_1023, PP_2112,...”
- Pathway-Consensus Approach to Metabolic Network Reconstruction for Pseudomonas putida KT2440 by Systematic Comparison of Published Models
Yuan, PloS one 2017 - “...the updated iJP962 model. From the annotation information we found the right gene should be PP_5347 annotated as pyruvate carboxyl transferase subunit A (EC 6.4.1.1). Altogether we added 155 genes and 74 reactions. The final pathway-consensus metabolic model PpuQY1140 contains 1140 genes, 1171 reactions and 1104...”
- Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol
Beckers, Microbial cell factories 2016 - “...0.2 0.11 0.91 1.01 acsA PP4487 Acetyl-CoA synthetase 0.13 0.23 0.15 3.56 accC - 2 PP5347 Pyruvate carboxylase 0.22 0.27 0.12 0.07 ppsA PP2082 Phosphoenolpyruvate synthase 0.10 0.22 0.12 0.09 ppc PP1505 Phosphoenolpyruvate carboxylase 0.09 0.06 0.10 0.50 PP0154 Acetyl-CoA hydrolase 0.21 0.23 1.42 1.41 TCA...”
- Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida
Borrero-de, Microbial cell factories 2014 - “...dehydrogenase 0.7 0.7 PP0545 Aldehyde dehydrogenase 0.1 -1.0 acsA PP4487 Acetyl-CoA synthetase 0.8 1.4 accC-2 PP5347 Pyruvate carboxylase 0.5 0.2 ppsA PP2082 Phosphoenolpyruvate synthase 0.5 0.4 ppc PP1505 Phosphoenolpyruvate carboxylase 0.5 0.4 TCA cycle gltA PP4194 Citrate synthase 0.7 0.7 acnA PP2112 Aconitate hydratase 0.4 -0.4...”
- “...dehydrogenase 0.5 -1.2 PP0545 Aldehyde dehydrogenase 0.5 -0.9 acsA PP4487 Acetyl-CoA synthetase -0.3 -0.6 accC-2 PP5347 Pyruvate carboxylase 0.8 2.3 ppsA PP2082 Phosphoenolpyruvate synthase 0.2 -0.1 ppc PP1505 Phosphoenolpyruvate carboxylase 0.0 1.4 TCA cycle gltA PP4194 Citrate synthase -0.2 0.7 acnA PP2112 Aconitate hydratase 0.8 -0.5...”
- “...were detected for the pyruvate metabolism, where the genes encoding pyruvate dehydrogenase (PP_0554), pyruvate carboxylase (PP_5347) and phosphoenoylpyruvate (PP_1505) showed differential expression levels (Table 5 ). At least for the wild type strain harboring the overexpressed pgl a problematic rearrangement of pyruvate metabolization occurred which might...”
- New insights on the reorganization of gene transcription in Pseudomonas putida KT2440 at elevated pressure
Follonier, Microbial cell factories 2013 - “...-1.54 3.7 E-03 PP_5346 pyruvate carboxylase subunit B oadA -1.58 1.7 E-02 -1.51 1.1 E-01 PP_5347 pyruvate carboxylase subunit A accC-2 -2.23 1.9 E-03 -2.41 5.6 E-03 PP_5075 glutamate synthase subunit beta gltD +1.69 1.3 E-02 PP_5409 glucosamine--fructose-6-phosphate aminotransferase glmS +1.54 1.3 E-02 Electron transport PP_4265...”
- Regulatory tasks of the phosphoenolpyruvate-phosphotransferase system of Pseudomonas putida in central carbon metabolism
Chavarría, mBio 2012 - “...Mdh, malate dehydrogenase (PP_0654); OadA, pyruvate carboxylase subunit B (PP_5346); AccC-2, pyruvate carboxylase subunit A (PP_5347); MaeB, malic enzyme (PP_5085); Ppc, phosphoenolpyruvate carboxylase (PP_1505). The 16 sites of the network where metabolic fluxes could be calculated as explained in the text are indicated. The first question...”
- “...In P. putida , two genes encode the corresponding subunits of pyruvate carboxylase: accC -2 (PP_5347) and oadA (PP_5346). To determine whether the effect of PtsN (EIIA Ntr ) on the pyruvate shunt reflects a direct or indirect action of the phosphotransferase on its constituent enzymatic...”
- Computational prediction of the Crc regulon identifies genus-wide and species-specific targets of catabolite repression control in Pseudomonas bacteria
Browne, BMC microbiology 2010 - “...PP_4636 PP_4636 acetyl-CoA acetyltransferase 3.6 2.3 hupA PP_5313 PP_5313 probable DNA-binding protein 3.8 nd accC-2 PP_5347 PP_5347 acetyl-CoA carboxylase subunit A 2.4 nd Genes differentially regulated, based on transcriptome and proteome data, in rich media in a crc mutant of P. putida KT2442 [ 26 ]...”
- Convergent peripheral pathways catalyze initial glucose catabolism in Pseudomonas putida: genomic and flux analysis
del, Journal of bacteriology 2007 - “...functions PP1014 PP2968 PP3384 PP3418 PP3555 PP4073 PP4488 PP5346 PP5347 a Change (n-fold). Gene gcd oprB-2 gap-1 edd glk zwf-1 eda kguD kguT kguK kguE gadC gad...”
- “...minimal medium with glucose. All mutants, except for PP5347, exhibited growth characteristics similar to those of the wildtype strain in terms of growth...”
SAR1685 putative biotin carboxylase subunit of acetyl-CoA carboxylase from Staphylococcus aureus subsp. aureus MRSA252
50% identity, 91% coverage
- A functional menadione biosynthesis pathway is required for capsule production by Staphylococcus aureus
Altwiley, Microbiology (Reading, England) 2021 - “...membrane protein 0.039515902 SAR1141 Similar to Staphylococcus aureus exotoxin 0.039515902 SAR1670 conserved hypothetical protein 0.039515902 SAR1685 putative biotin carboxylase subunit of acetyl-CoA carboxylase 0.039515902 lysS lysyl-tRNA synthetase 0.039515902 Intergenic between lysS and SAR1413 0.039515902 Intergenic between SAR1326 and SAR1327 0.039515902 pbp4 penicillin-binding protein 4 0.039515902 SAR0880...”
PVLB_25330 acetyl-CoA carboxylase biotin carboxylase subunit from Pseudomonas sp. VLB120
49% identity, 94% coverage
A0A8I3S647 Propionyl-CoA carboxylase alpha chain, mitochondrial from Canis lupus familiaris
47% identity, 60% coverage
PCCA / P05165 propionyl-CoA carboxylase α chain, mitochondrial (EC 6.4.1.3) from Homo sapiens (see 4 papers)
PCCA_HUMAN / P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Homo sapiens (Human) (see 11 papers)
P05165 propionyl-CoA carboxylase (subunit 1/2) (EC 6.4.1.3) from Homo sapiens (see paper)
NP_000273 propionyl-CoA carboxylase alpha chain, mitochondrial isoform a precursor from Homo sapiens
47% identity, 61% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites (PubMed:6765947, PubMed:8434582). Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:10101253, PubMed:6765947, PubMed:8434582). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA at a much lower rate (PubMed:6765947). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits (PubMed:20725044, PubMed:6765947). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). Interacts with SIRT3 and SIRT5 (PubMed:23438705). - Raphe and ventrolateral medulla proteomics in sudden unexplained death in childhood with febrile seizure history
Leitner, Acta neuropathologica 2024 - “...1.3 RPL12 60S ribosomal protein L12 P30050 4.07E04 1.4 PCCA Propionyl-CoA carboxylase alpha chain, mitochondrial P05165 5.33E04 1.2 VAMP2 Vesicle-associated membrane protein 2 P63027 6.15E04 1.7 CCT3 T-complex protein 1 subunit gamma P49368 7.86E04 1.1 ATP6V1D V-type proton ATPase subunit D Q9Y5K8 8.20E04 1.2 FARSA PhenylalaninetRNA...”
- Generation and Characterization of Trastuzumab/Pertuzumab-Resistant HER2-Positive Breast Cancer Cell Lines.
Sanz-Álvarez, International journal of molecular sciences 2023 - “...0.32 1.65 10 28 426 P18564 ITGB6 Integrin beta 6 0.33 1.59 3 5 103 P05165 PCCA Propionyl-CoA carboxylase alpha chain, mitochondrial 0.34 1.57 7 10 144 Q8IW45 NAXD ATP-dependent (S)-NAD(P)H-hydrate dehydratase 0.37 1.44 5 10 163 Q96AY3 FKBP10 Peptidyl-prolyl cis/trans isomerase FKBP10 0.38 1.41 14...”
- Interactome Analysis of the ER Stress Sensor Perk Uncovers Key Components of ER-Mitochondria Contact Sites and Ca2+ Signalling
Sassano, Contact (Thousand Oaks (Ventura County, Calif.)) 2021 - “...94 31 100% 100% Propionyl-CoA carboxylase alpha chain, mitochondrial OS=Homo sapiens OX=9606 GN=PCCA PE=1 SV=4 P05165 PCCA 80 kDa 117 49 114 45 100% 100% Pyruvate carboxylase, mitochondrial OS=Homo sapiens OX=9606 GN=PC PE=1 SV=2 P11498 PC 130 kDa 122 54 121 49 100% 100% Considering our...”
- Proteome biology of primary colorectal carcinoma and corresponding liver metastases.
Fahrner, Neoplasia (New York, N.Y.) 2021 - “...0.917 2.35E-04 7.92E-03 PLG Plasminogen O95671 1.631 1.89E-06 4.55E-04 ASMTL Probable bifunctional dTTP/UTP pyrophosphatase/methyltransferase protein P05165 0.69 2.54E-03 3.95E-02 PCCA Propionyl-CoA carboxylase alpha chain P00734 0.896 2.93E-03 4.29E-02 F2 Prothrombin P11498 2.044 7.18E-07 2.77E-04 PC Pyruvate carboxylase Q99575 0.746 4.31E-04 1.14E-02 POP1 Ribonucleases P/MRP protein subunit...”
- Cigarette smoke extract stimulates bronchial epithelial cells to undergo a SUMOylation turnover.
Zhou, BMC pulmonary medicine 2020 - “...Down NACA P15104 Glutamine synthetase 0.336 Down GLUL P20290 Transcription factor BTF3 0.337 Down BTF3 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial 0.345 Down PCCA P08865 40S ribosomal protein SA 0.407 Down RPSA P16401 Histone H1.5 0.407 Down HIST1H1B P67809 Nuclease-sensitive element-binding protein 1 0.426 Down YBX1...”
- Investigating the structural impacts of a novel missense variant identified with whole exome sequencing in an Egyptian patient with propionic acidemia.
Ibrahim, Molecular genetics and metabolism reports 2020 - “...The protein sequence for the PCCA was obtained from the UniProt database with the ID P05165 [ 11 ]. The PCCA protein lacks the crystal structure in the public databases to date. Henceforth, the protein sequence obtained from the UniProt database was used to build the...”
- SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells.
Kim, Journal of immunology (Baltimore, Md. : 1950) 2018 - “...MCCA_HUMAN 249.9351293 (Q96RQ3) Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Mitochondrial enzymes with Biotin cofactor PCCA_HUMAN 93.580363 (P05165) Propionyl-CoA carboxylase alpha chain, mitochondrial Mitochondrial enzymes with Biotin cofactor DLDH_HUMAN 165.104467 (P09622) Dihydrolipoyldehydrogenase, mitochondrial Photo damage-related proteins ARGI1_HUMAN 349.6449536 (P05089) Arginase-1 Photo damage-related proteins CASPE_HUMAN 198.6111486 (P31944) Caspase-14 Heterogeneous...”
- Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction
Raphael, Journal of translational medicine 2016 - “...YP010 Q96M66 Putative uncharacterized protein FLJ32790 PSMD4 P55036 26S proteasome non-ATPase regulatory subunit 4 PCCA P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial TCPR2 O15040 Tectonin beta-propeller repeat-containing protein KPRP Q5T749 Keratinocyte proline-rich protein GTPB5 Q9H4K7 GTP-binding protein 5 CV031 O95567 Uncharacterized protein C22orf31 TFB2M Q9H5Q4 Dimethyladenosine transferase...”
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- The mitochondrial carboxylase PCCA interacts with Listeria monocytogenes phospholipase PlcB to modulate bacterial survival.
Wang, Applied and environmental microbiology 2024 - GeneRIF: The mitochondrial carboxylase PCCA interacts with Listeria monocytogenes phospholipase PlcB to modulate bacterial survival.
- A novel small molecule approach for the treatment of propionic and methylmalonic acidemias.
Armstrong, Molecular genetics and metabolism 2021 - GeneRIF: A novel small molecule approach for the treatment of propionic and methylmalonic acidemias.
- Functional Analysis of the PCCA and PCCB Gene Variants Predicted to Affect Splicing.
Bychkov, International journal of molecular sciences 2021 - GeneRIF: Functional Analysis of the PCCA and PCCB Gene Variants Predicted to Affect Splicing.
- Identification of 34 novel mutations in propionic acidemia: Functional characterization of missense variants and phenotype associations.
Rivera-Barahona, Molecular genetics and metabolism 2018 (PubMed)- GeneRIF: his work represents a large-scale update on pathogenic mutations in the PCCA and PCCB genes causing Propionic acidemia (PA), and confirms previous reports indicating a major causative role of mutation-induced protein destabilization
- Seventeen Novel Mutations in PCCA and PCCB Genes in Indian Propionic Acidemia Patients, and Their Outcomes.
Gupta, Genetic testing and molecular biomarkers 2016 (PubMed)- GeneRIF: The majority of patients had mutations in the PCCA gene (18/25). A total of 26 mutations were noted: 20 in the PCCA gene and 6 in PCCB gene. Seventeen mutations were novel (14 in PCCA and 3 in PCCB). The SNP c.937C>T (p.Arg313Ter), was noted in 9/36 (25%) alleles in the PCCA gene
- Frameshift mutations of OGDH, PPAT and PCCA genes in gastric and colorectal cancers.
Jo, Neoplasma 2016 (PubMed)- GeneRIF: The data indicate that amino acid/nucleotide metabolism-related genes OGDH, PPAT and PCCA acquire somatic mutations in microsatellite instability-high gastric cancers and colorectal cancers and that mutational intratumoral heterogeneity may occur in at least some of these tumors.
- [Analysis of PCCA and PCCB gene mutations in patients with propionic acidemia].
Chen, Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 2015 (PubMed)- GeneRIF: Ten propionic acidemia mutations were confirmed, including 8 affecting the PCCA gene and 2 affecting the PCCB gene
- Clinical characteristics and mutation analysis of propionic acidemia in Thailand.
Vatanavicharn, World journal of pediatrics : WJP 2014 (PubMed)- GeneRIF: Mutation analysis of the PCCA gene identified homozygous c.1284+1G>A in patient 1, c.230G>A (p.R77Q) and c.1855C>T (p.R619X) in patient 2, homozygous c.2125T>C (p.S709P) in patient 3, and only one mutant allele, c.231+1G>T in patient 4.
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DDB_G0275355 propanoyl-CoA:carbon dioxide ligase alpha subunit from Dictyostelium discoideum AX4
44% identity, 60% coverage
Msed_0147 / A4YD22 acyl CoA carboxylase biotin carboxyl carrier protein subunit from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) (see paper)
Q8J2Z4 biotin carboxylase (EC 6.3.4.14); acetyl-CoA carboxylase (EC 6.4.1.2) from Metallosphaera sedula (see 2 papers)
A4YD22 Biotin carboxylase from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
Msed_0147 Carbamoyl-phosphate synthase L chain, ATP-binding from Metallosphaera sedula DSM 5348
45% identity, 86% coverage
- Genome analysis of the thermoacidophilic archaeon Acidianus copahuensis focusing on the metabolisms associated to biomining activities
Urbieta, BMC genomics 2017 - “...(position) Protein Function HhcR A4YIR2 115 EZQ01893 115 1.00E -46 64 HhcR transcription factor accA A4YD22 510 EZQ11106 507 0 78 + (46) Acetyl-CoA/propionyl-CoA carboxylase, alpha subunit accB A4YD23 167 EZQ11107 167 2.00E -84 64 - Acetyl-CoA/propionyl-CoA carboxylase, beta subunit accC A4YGI1 524 EZQ11108 523 0...”
- Ancillary contributions of heterologous biotin protein ligase and carbonic anhydrase for CO2 incorporation into 3-hydroxypropionate by metabolically engineered Pyrococcus furiosus
Lian, Biotechnology and bioengineering 2016 - “...to 3-hydroxypropionate (see Figure 1 ) and involves three enzymes: the heterotrimeric acetyl-CoA/propionyl-CoA carboxylase (ACC) (Msed_0147, 0148, and 1375) ( Hgler et al. 2003 ); malonyl-CoA reductase (MCR) (Msed_0709) ( Alber et al. 2006 ); and malonic semialdehyde reductase (MSR) (Msed_1993) ( Kockelkorn and Fuchs 2009...”
- “...C-terminal 6His-tag. The M. sedula genes encoding the -, -, and -subunits of the carboxylase (Msed_0147, Msed_1048, and Msed_1375) were cloned by a ligation-independent method using pET46 Ek/LIC, pRSF-2 Ek/LIC, and pCDF-2 Ek/LIC (EMD4 Biosciences), respectively, which are compatible vectors enabling co-expression of these genes in...”
- Novel Transcriptional Regulons for Autotrophic Cycle Genes in Crenarchaeota
Leyn, Journal of bacteriology 2015 - “...dhl3 naaB hhcR hyp1-2 hyp3 hyp4 hyp5 Msed_0147- Msed_0148 Msed_1375 SSO2178 Msed_1993 Msed_1456 Msed_1426 Msed_0639 Msed_0638 Msed_2055 Msed_1424 Msed_1422...”
- Bioprocessing analysis of Pyrococcus furiosus strains engineered for CO₂-based 3-hydroxypropionate production
Hawkins, Biotechnology and bioengineering 2015 - “...was examined. P. furiosus MW56, the COM1 strain engineered to contain genes encoding acetyl-CoA carboxylase (Msed_0147, Msed_0148, Msed_1375), malonyl-CoA reductase (Msed_0709), and malonate semialdehyde reductase (Msed_1993), was compared to the parent strain COM1; both strains were grown in 1L bottles at 95C on complex media containing...”
- “...enzymes of the M. sedula 3HP/4HB cycle. Enzyme Abbrev Enzyme ORF Reference ACC/PCC Acetyl-CoA/Propionyl-CoA carboxylase Msed_0147 NCE (Hgler et al., 2003) Msed_0148 Msed_1375 MCR/SCR Malonyl-CoA/Succinyl-CoA reductase (NADPH) Msed_0709 R (Kockelkorn and Fuchs, 2009) MSR Malonate semialdehyde reductase (NADPH) Msed_1993 R (Kockelkorn and Fuchs, 2009) CA Carbonic...”
- Propionyl coenzyme A (propionyl-CoA) carboxylase in Haloferax mediterranei: Indispensability for propionyl-CoA assimilation and impacts on global metabolism
Hou, Applied and environmental microbiology 2015 - “...of ACC/ PCC from M. sedula are encoded by Msed_0147, Msed_0148, and Msed_1375, respectively (9). Based on their amino acid sequences, in silico analysis of the...”
- Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Hawkins, Applied and environmental microbiology 2014 - “...dehydrogenase (NADH) Acetoacetyl-CoA -ketothiolase Msed_0147, Msed_0148, Msed_1375 Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639...”
- “...genes encoding acetyl-CoA/propionyl-CoA carboxylase (ACC) (Msed_0147, Msed_0148, Msed_1375; 7- to 30-fold), 4-hydroxybutyryl-CoA dehydratase (Msed_1321;...”
- Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide
Keller, Proceedings of the National Academy of Sciences of the United States of America 2013 - “...as E1 (acetyl/propionyl-CoA carboxylase, encoded by Msed_0147, Msed_0148, and Msed_1375), E2 (malonyl/succinylCoA reductase, Msed_0709), and E3 (malonate...”
- “...E1 is acetyl/propionyl-CoA carboxylase (, encoded by Msed_0147, Msed_0148, Msed_1375), E2 is malonyl/succinyl-CoA reductase (Msed_0709), and E3 is malonate...”
- Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea
Hawkins, The Journal of biological chemistry 2013 - “...February 11, 2017 E11 E12 E13 ORF Msed_0147 Msed_0148 Msed_1375 Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639 Msed_0638 Msed_2055 Msed_1424...”
- “...and -subunits of acetyl-CoA/ propionyl-CoA carboxylase (Msed_0147- 0148) were up-regulated 18- and 29-fold, respectively, whereas the 4-hydroxybutyryl-CoA...”
- Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle
Han, Applied and environmental microbiology 2012 - “...HPCS HPCD ACR MCE MCM MCM Msed_0147() Msed_0148() Msed_1375() Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639 Msed_0638 Msed_2055...”
- “...(see Table 1). (B) Heat plot showing ACC (Msed_0147, Msed_0148, Msed_1375), MCE (Msed_0639), and MCM (Msed_0638, Msed_2055) under autotrophic (columns A) and...”
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7kctA / D3DJ42 Crystal structure of the hydrogenobacter thermophilus 2-oxoglutarate carboxylase (ogc) biotin carboxylase (bc) domain dimer in complex with adenosine 5'-diphosphate magnesium salt (mgadp), adenosine 5'- diphosphate (adp, and bicarbonate anion (hydrogen carbonate/hco3-) (see paper)
49% identity, 97% coverage
- Ligands: adenosine-5'-diphosphate; magnesium ion; bicarbonate ion (7kctA)
CJU75_13125 acetyl-CoA carboxylase biotin carboxylase subunit from Pseudomonas fragi
49% identity, 94% coverage
Pden_3684 Carbamoyl-phosphate synthase L chain, ATP-binding from Paracoccus denitrificans PD1222
PDEN_RS18285 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Paracoccus denitrificans
45% identity, 64% coverage
MAB_4539c Putative acyl-CoA carboxylase alpha subunit AccA from Mycobacterium abscessus ATCC 19977
49% identity, 68% coverage
C3K4F7 Biotin carboxylase from Pseudomonas fluorescens (strain SBW25)
48% identity, 94% coverage
PFL_6158 acetyl-CoA carboxylase, biotin carboxylase from Pseudomonas fluorescens Pf-5
48% identity, 94% coverage
Saci_0260 pyruvate carboxylase subunit A from Sulfolobus acidocaldarius DSM 639
45% identity, 86% coverage
- Versatile Genetic Tool Box for the Crenarchaeote Sulfolobus acidocaldarius
Wagner, Frontiers in microbiology 2012 - “...identity was confirmed by mass spectrometry. Two other proteins were co-purified that were identified as Saci_0260 and Saci_0262, subunits of the pyruvate carboxylase. These two proteins seem to be biotinylated proteins that bind to the chromatography material. The same proteins have been observed during Strep affinity...”
Q9FAF5 biotin carboxylase (EC 6.3.4.14) from Myxococcus xanthus (see paper)
51% identity, 80% coverage
BLU65_RS05250 acetyl-CoA carboxylase biotin carboxylase subunit from Pseudomonas lini
48% identity, 94% coverage
- Transcriptome Profiling Analysis of Phosphate-Solubilizing Mechanism of Pseudomonas Strain W134
Wang, Microorganisms 2022 - “...three P sources, resulting in twenty genes. The results showed that the expression levels of BLU65_RS05250, BLU65_RS11635, and gltA in group B were higher than in groups A and C, while the expression levels of BLU65_RS00705, aceE, aceF, and BLU65_RS04330 in group C were significantly higher...”
- “...down-regulated in the other two groups. Further analysis of significantly upregulated genes revealed that BLU65_RS00350, BLU65_RS05250, gltA, and oadA were significantly upregulated only in group B. Among them, BLU65_RS05250 and oadA were both related to pyruvate carboxylase (the only difference was their subunits), and pyruvate carboxylase...”
LOC21385626 methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial from Morus notabilis
46% identity, 61% coverage
- Genome-Wide Identification of Candidate Genes Associated with Heat Stress in Mulberry (Morus alba L.)
Jin, Current issues in molecular biology 2023 - “...dehydrogenase (LOC21408668), branched-chain amino acid aminotransferase 1 (LOC21390509), isovaleryl-CoA dehydrogenase (LOC21397545), methylcrotonoyl-CoA carboxylase subunit alpha (LOC21385626), probable enoyl-CoA hydratase 1 (LOC21392597), aldehyde dehydrogenase family 3 (LOC21396538) and alanine--glyoxylate aminotransferase (LOC21404820). In plants, the catabolism of amino acids is extremely important in metabolic stress (e.g., when there...”
MCCA_ORYSJ / Q2QMG2 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 from Oryza sativa subsp. japonica (Rice) (see paper)
47% identity, 60% coverage
- function: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
catalytic activity: 3-methyl-(2E)-butenoyl-CoA + hydrogencarbonate + ATP = 3- methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+) (RHEA:13589)
cofactor: biotin
cofactor: Mn(2+) (Binds 2 manganese ions per subunit.)
subunit: Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits.
SACE_6509 acetyl/propionyl-CoA carboxylase alpha subunit from Saccharopolyspora erythraea NRRL 2338
51% identity, 72% coverage
- Polyketide Starter and Extender Units Serve as Regulatory Ligands to Coordinate the Biosynthesis of Antibiotics in Actinomycetes
Wu, mBio 2021 - “...genes encode ACC enzymes; SACE_3398-3399 , SACE_7038-7039 , SACE_3241-3242 , SACE_3400 , SACE_4237 , and SACE_6509 genes encode ACC and/or PCC enzymes; SACE_0632 , SACE_0633 , and SACE_0649 genes encode CS enzymes. Mean values of 3 measurements are shown with SDs. *, P< 0.05; **, P<...”
- “...PCC genes ( SACE_0026-0028 , SACE_3241-3242 , SACE_3398-3399 , SACE_3400 , SACE_3856 , SACE_4237 , SACE_6509 , and SACE_7038-7039 ) and three citrate synthase (CS) genes ( SACE_0632 , SACE_0633 , and SACE_0649 ) between A226 and A226 acrT ( 28 , 29 ). The results...”
- The Expression of NOX From Synthetic Promoters Reveals an Important Role of the Redox Status in Regulating Secondary Metabolism of Saccharopolyspora erythraea
Li, Frontiers in bioengineering and biotechnology 2020 - “...the NOX-expression strains at transcription level ( Liao et al., 2015 ). Furthermore SACE_3400 and SACE_6509 coding for acetyl-CoA carboxylase were repressed gradually in the NOX-expression strains. The repression of SACE_3400 and SACE_6509 could reduce the accumulation of malonyl-CoA from acetyl-CoA. The transcriptional changes of these...”
- TetR Family Transcriptional Regulator PccD Negatively Controls Propionyl Coenzyme A Assimilation in Saccharopolyspora erythraea
Xu, Journal of bacteriology 2017 - “...carboxylases are 118 annotated, 119 SACE_7038-7039, SACE_4237, and SACE_6509 (Fig. S1). The co-transcription of 120 genes in each locus was investigated (Fig....”
CPI83_20020 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Rhodococcus sp. H-CA8f
51% identity, 65% coverage
PCCA_MOUSE / Q91ZA3 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Mus musculus (Mouse) (see paper)
46% identity, 61% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl- CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PCCA/alpha subunits and 6 PCCB/beta subunits (By similarity). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). Interacts with SIRT3 and SIRT5 (PubMed:23438705). - ISG15 Is a Novel Regulator of Lipid Metabolism during Vaccinia Virus Infection.
Albert, Microbiology spectrum 2022 - “...Q99MN9 Pccb Propionyl-CoA carboxylase beta chain, mitochondrial 10.02 P51174 Acadl Long-chain-specific acyl-CoA dehydrogenase, mitochondrial 8.64 Q91ZA3 Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial 8.63 Q9EPL9 Acox3 Peroxisomal acyl-coenzyme A oxidase 3 8.62 Q8QZT1 Acat1 Acetyl-CoA acetyltransferase, mitochondrial 7.71 Q9Z2Z6 Slc25a20 Mitochondrial carnitine/acylcarnitine carrier protein 6.67 Q9JHI5 Ivd...”
- A Quantitative Proteomics Approach to Gain Insight into NRF2-KEAP1 Skeletal Muscle System and Its Cysteine Redox Regulation
Abu, Genes 2021 - “...Protein Log 2 FC Nrf2-KO Log 2 FC Keap1-KO Q9CXV1 Sdhd 1.87569 a 1.48549 b Q91ZA3 Pcca 1.69494 a 1.65623 b Q70IV5 Synm 1.67506 a 1.58577 b Q9JMH6 Txnrd1 1.38643 b 1.5322 a P15626 Gstm2 2.18014 b 1.4955 a Q9D6Y9 Gbe1 8.56657 b 3.97791 a a...”
- Action potential-coupled Rho GTPase signaling drives presynaptic plasticity
O'Neil, eLife 2021 - “...expression levels were intensity-scaled to the endogenously biotinylated proteins, pyruvate carboxylase (Q05920) and propionyl-CoA carboxylase (Q91ZA3). Imputation of missing values was performed after normalization ( Karpievitch et al., 2012 ) using the MinDet method ( Lazar et al., 2016 ). Missing values were replaced by the...”
- The role of cellular prion protein in lipid metabolism in the liver
Arora, Prion 2020 - “...155 1.59 0.031 5.16 4 Not identified 4.65 0.025 5 Propionyl-CoA carboxylase alpha chain, mitochondrial Q91ZA3 79.92 17.70% 17 1.58 0.04 6.04 Male 14-month-old mice 6 U5 small nuclear ribonucleoprotein 200 kDa helicase Q6P4T2 244.55 12.30% 36 1.77 0.012 5.73 7 3-mercaptopyruvate sulfurtransferase Q99J99 33.02 67.30%...”
- Environmental Enrichment Upregulates Striatal Synaptic Vesicle-Associated Proteins and Improves Motor Function
Song, Frontiers in neurology 2018 - “...gamma PIP5K1C 1.51 P32037 Solute carrier family 2, facilitated glucose transporter member 3 SLC2A3 1.51 Q91ZA3 Propionyl-CoA carboxylase alpha chain, mitochondrial PCCA 1.51 E9Q035 Protein Gm20425 GM20425 1.51 Q7TMY8-4 Isoform 4 of E3 ubiquitin-protein ligase HUWE1 HUWE1 1.51 D3YU23 Latrophilin-3 LPHN3 1.51 Q8C1A5 Thimet oligopeptidase THOP1...”
- Adenosine A1 receptor activation increases myocardial protein S-nitrosothiols and elicits protection from ischemia-reperfusion injury in male and female hearts
Shao, PloS one 2017 - “...1 P09542 191 85, 191 ND 85, 191 ND ND Propionyl CoA carboxylase alpha chain Q91ZA3 107 107 107 107 107 107 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 O55143 349, 364, 447, 471 344, 349, 364, 447, 471, 635, 669 364, 635 344, 364, 447, 635, 998...”
- Characterization of the sex-dependent myocardial S-nitrosothiol proteome
Shao, American journal of physiology. Heart and circulatory physiology 2016 - “...75 (ND), 92 (ND), 727 (ND) 85 (ND) 85 (ND), 191 (ND) Q91ZA3 O55143 107 (ND) 344 (3.02) P07724 77 (ND), 289 (ND) 77 (ND), 289 (ND), 301 (ND), 302 (ND), 501 (ND),...”
- Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2.
Zeng, Oxidative medicine and cellular longevity 2015 - “...(25) 71270 6.23 Guanylate-binding protein 4 4 P12265 (BGLR_MOUSE) 143 (28) 74648 6.22 Beta-glucuronidase 5 Q91ZA3 (PCCA_MOUSE) 80 (27) 80498 6.83 Propionyl-CoA carboxylase alpha chain, mitochondrial 6 P58252 (EF2_MOUSE) 264 (27) 96222 6.41 Elongation factor 2 7 N/A e 8 P60710 (ACTB_MOUSE) 79 (26) 42052 5.29...”
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XNRR2_4211 acetyl-CoA carboxylase biotin carboxylase subunit from Streptomyces albidoflavus
54% identity, 65% coverage
2OCS_HYDTT / D3DJ42 2-oxoglutarate carboxylase small subunit; 2-oxoglutarate carboxylase beta subunit; EC 6.4.1.7 from Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6) (see 2 papers)
49% identity, 93% coverage
- catalytic activity: hydrogencarbonate + 2-oxoglutarate + ATP = (S)-oxalosuccinate + ADP + phosphate + H(+) (RHEA:20425)
cofactor: Mg(2+) Mn(2+) Co(2+)
subunit: Heterohexadecamer of 8 large subunits and 8 small subunits.
F1KUZ6 Propionyl-CoA carboxylase alpha chain, mitochondrial from Ascaris suum
46% identity, 60% coverage
pccA / Q3J4D9 propionyl-CoA carboxylase α subunit (EC 6.4.1.3) from Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (see paper)
46% identity, 66% coverage
Dshi_0723 Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) from Dinoroseobacter shibae DFL-12
47% identity, 62% coverage
- mutant phenotype: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (PROPIONYL-COA-CARBOXY-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
MAB_3643 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycobacteroides abscessus ATCC 19977
MAB_3643 Probable bifunctional protein acetyl-/propionyl-CoA carboxylase (alpha chain) AccA3 from Mycobacterium abscessus ATCC 19977
49% identity, 72% coverage
SPO2789 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Ruegeria pomeroyi DSS-3
49% identity, 68% coverage
- Diel investments in metabolite production and consumption in a model microbial system
Uchimiya, The ISME journal 2022 - “...Glycine-betaine transporter 1.6 (n.s.) [ 25 ] Amino Acid Leucine SPO2793 ivD isovaleryl-CoA dehydrogenase 2.6 SPO2789 mccA methylcrotonyl-CoA carboxylase, alpha subunit 3.1 SPO2790 mccB methylcrotonyl-CoA carboxylase, beta subunit 3.4 SPO0390 glutamate/leucine/phenylalanine/valine dehydrogenase 1.2 (n.s.) Amino acid derivative Ectoine/ 5-hydroxyectoine SPO1146 uehB TRAP transporter, small integral membrane...”
lp_1678 acetyl-CoA carboxylase, biotin carboxylase subunit from Lactobacillus plantarum WCFS1
F9UP42 biotin carboxylase from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
lp_1678 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Lactiplantibacillus plantarum WCFS1
46% identity, 97% coverage
- Transcriptional analysis of the molecular mechanism underlying the response of Lactiplantibacillus plantarum to lactic acid stress conditions
Jang, Heliyon 2023 - “...4.07E-82 2.61E-79 acyl-carrier proteinreductase lp_1681 COG0623 accC2 5.06 8.15E-64 3.49E-61 5.81 5.19E-79 2.22E-76 acetyl-CoA carboxylase lp_1678 COG0439 accB2 4.99 3.46E-60 9.89E-58 5.66 1.07E-72 3.06E-70 acetyl-CoA carboxylase lp_1676 COG0511 fabG1 4.85 1.73E-62 5.57E-60 5.64 2.75E-78 1.01E-75 3-oxoacyl-ACP reductase lp_1674 COG1028 fabF 4.90 1.40E-68 1.20E-65 5.63 6.29E-85 5.39E-82...”
- “...Both acidic conditions increased the expression of acetyl-CoA carboxylase, acyl carrier proteins (lp_1680, lp_1679, lp_1676, lp_1678), oxoacyl-ACP reductase (lp_1674), oxoacyl-ACP synthase (lp_1675, lp_1671), acyl-CoA hydrolase (lp_1686), and membrane proteins (lp_3177, lp_2949, lp_1684). Interestingly, the expression of lactate racemization-related genes (lp_0105, lp_0104, lp_0106, and lp_1017) increased only...”
- Oleuropein Transcriptionally Primes Lactobacillus plantarum to Interact With Plant Hosts
Santamaría, Frontiers in microbiology 2019 - “...addition, genes coding for enzymes of the acetyl-CoA complex ( accB2 or lp_1676, accC2 or lp_1678, accD2 or lp_1679 ), which use acetyl-CoA to initiate the fatty acid biosynthesis (fab), and the acetate kinase ( lp_0210 ) which forms acetate from acetyl-P, were downregulated. The DAVID...”
- “...( fabZ1 or lp_1670 , fabD or lp_1673 , accB2 or lp_1676 , accC2 or lp_1678 , accD2 or lp_1679 ) and elongation ( fabH2 or lp_1670 , fabG1 or lp_1674 , fabF or lp_1675 , fabZ2 or lp_1677 , fabI or lp_1681 ) steps of...”
- Response of Lactobacillus plantarum WCFS1 to the Gram-Negative Pathogen-Associated Quorum Sensing Molecule N-3-Oxododecanoyl Homoserine Lactone
Spangler, Frontiers in microbiology 2019 - “...dehydratase 0.51 0.81 F9UP40_LACPL lp_1676 accB2 AcCoA carboxylase biotin carboxyl carrier protein 0.59 1.07 F9UP42_LACPL lp_1678 accC2 AcCoA carboxylase, biotin carboxylase subunit 0.78 0.52 ACCD2_LACPL lp_1679 accD2 AcCoA carboxylase carboxyl transferase subunit beta 0.80 0.66 F9UP44_LACPL lp_1680 accA2 AcCoA carboxylase carboxyl transferase subunit alpha 1.07 0.42...”
- Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarrays
Saulnier, Applied and environmental microbiology 2007 - “...and phospholipid metabolism lp_1675 lp_1673 lp_1674 lp_1678 lp_1680 lp_1672 lp_1676 lp_1679 lp_1671 lp_1681 lp_1682 lp_1670 3-Oxoacyl-[acyl-carrier protein]...”
- Identification of key proteins and pathways in cadmium tolerance of Lactobacillus plantarum strains by proteomic analysis.
Zhai, Scientific reports 2017 - “...pyrR1; pyrimidine operon regulator 1.62 F9UNA0 dgk2; Deoxynucleoside kinase 2.04 Lipid metabolism Fatty acid biosynthesis F9UP42 accC2; acetyl-CoA carboxylase, biotin carboxylase subunit 1.90 Q88WG0 accD2; acetyl-CoA carboxylase, carboxyl transferase subunit beta 1.84 F9UP44 accA2; acetyl-CoA carboxylase, carboxyl transferase subunit alpha 1.98 Glycerol lipid metabolism F9USY0 dak1B;...”
RSP_2191 Propionyl-CoA carboxylase alpha subunit from Rhodobacter sphaeroides 2.4.1
46% identity, 63% coverage
Q612F5 Propionyl-CoA carboxylase alpha chain, mitochondrial from Caenorhabditis briggsae
45% identity, 60% coverage
DDD_1224 acetyl-CoA carboxylase biotin carboxylase subunit from Nonlabens dokdonensis DSW-6
46% identity, 97% coverage
AF_0220 acetyl-CoA carboxylase biotin carboxylase subunit from Archaeoglobus fulgidus DSM 4304
46% identity, 90% coverage
- A novel methoxydotrophic metabolism discovered in the hyperthermophilic archaeon Archaeoglobus fulgidus
Welte, Environmental microbiology 2021 - “...AF_0808), Llactate dehydrogenase LldD (AF_0807) and LldEFG (AF_0809AF_0811), lactate permease lctP (AF_0806), pyruvate carboxylase PycA (AF_0220) and oxaloacetate decarboxylase Oad (AF_1252), malate dehydrogenase MdhA (AF_0855) and malate oxidoreductase Mae (AF_1727), fumarate hydratase Fum1/2 (AF_1098/9), succinate dehydrogenase SdhAD (AF_06814), succinylCoA synthetase SucCD (AF_1539/40 & AF_2185/6), 2oxoglutarate/2oxoacid ferredoxin...”
FRAAL1210 biotin carboxylase; biotin carboxyl carrier protein (bifunctional protein) from Frankia alni ACN14a
49% identity, 73% coverage
- The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules
Pujic, Microorganisms 2022 - “...a methyl-crotonoyl-CoA carboxylase (FRAAL2343, FC = 0.15) and two propionyl-CoA carboxylases (FRAAL5672, FC = 0.05; FRAAL1210, FC = 0.68). The only overabundant one was a phosphoribosylaminoimidazole carboxylase (FRAAL6664, FC = 4.9) involved in the synthesis of purines. A COG analysis ( Figure 2 ) revealed that...”
O52602 biotin carboxylase (EC 6.3.4.14) from Sulfuracidifex metallicus (see 2 papers)
46% identity, 86% coverage
PCCA_CAEEL / Q19842 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Caenorhabditis elegans (see paper)
Q19842 propionyl-CoA carboxylase (subunit 2/2) (EC 6.4.1.3) from Caenorhabditis elegans (see paper)
NP_509293 Propionyl-CoA carboxylase alpha chain, mitochondrial from Caenorhabditis elegans
45% identity, 61% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl- CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA (By similarity). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)- ethylmalonyl-CoA (By similarity). Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: butanoyl-CoA + hydrogencarbonate + ATP = (2S)-ethylmalonyl-CoA + ADP + phosphate + H(+) (RHEA:59520)
cofactor: biotin
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
subunit: The holoenzyme is a dodecamer composed of 6 alpha subunits and 6 beta subunits (By similarity). Interacts with sir-2.2 and sir-2.3 (PubMed:23438705). - Propionyl-CoA carboxylase pcca-1 and pccb-1 gene deletions in Caenorhabditis elegans globally impair mitochondrial energy metabolism.
Chapman, Journal of inherited metabolic disease 2018 - GeneRIF: Two C. elegans model animals of propionic acidemia with single-gene pcca-1 or pccb-1 deletions have reduced lifespan with significantly reduced mitochondrial energy metabolism and increased oxidative stress.
- Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti--identification of stage-specific proteases
Soblik, Molecular & cellular proteomics : MCP 2011 - “...2e 104 No 317 12.0 3 9.17 25 SR00383 Propionyl Coenzyme A Carboxylase C. elegans NP_509293 2e 17 No 76 50.0 3 9.10 1 SR01608 EF hand family protein Brugia malayi XP_001901161 2e 37 Yes 158 69.0 8 23.28 2 SR03191 Prolyl endopeptidase Rattus norvegicus EDL99674...”
- Altered proteostasis in aging and heat shock response in C. elegans revealed by analysis of the global and de novo synthesized proteome
Liang, Cellular and molecular life sciences : CMLS 2014 - “...0.8 0.4 Q22111 mmaa-1 Methylmalonic aciduria type A protein 4 0.7 0.4 4 0.8 0.3 Q19842 pcca-1 Propionyl coenzyme-A carboxylase alpha subunit 4 0.8 0.6 Q9U2M4 Y38F1A.6 Ortholog, human isoform 1, HOT 1 0.6 0.3 Q9U2M4 Y38F1A.6 Ortholog, human isoform 1, HOT 5 0.8 0.3 Ribosomal...”
- Proteomic analysis of Oesophagostomum dentatum (Nematoda) during larval transition, and the effects of hydrolase inhibitors on development
Ondrovics, PloS one 2013 - “...Cyathostominae A7LGW9 741 83 9*10 77 2 / Propionyl-CoA carboxylase alpha chain / Caenorhabditis elegans Q19842 / / / 3 / Propionyl-CoA carboxylase alpha chain / C. elegans Q19842 / / / 4+5 Oden_isotig22486 Phosphoenolpyruvate carboxy kinase GTP Putative uncharacterized protein C. briggsae A8WMQ5 787 80...”
SSO2466 Biotin carboxylase a subunit of propionyl-CoA carboxylase (accC) from Sulfolobus solfataricus P2
46% identity, 86% coverage
CNC01910 methylcrotonoyl-Coenzyme A carboxylase 1 from Cryptococcus neoformans var. neoformans JEC21
48% identity, 57% coverage
E1BGC1 Methylcrotonyl-CoA carboxylase subunit 1 from Bos taurus
44% identity, 61% coverage
WP_202320510 acetyl-CoA carboxylase biotin carboxylase subunit from Archaeoglobus neptunius
47% identity, 90% coverage
PSPTO_2736 biotin carboxylase/biotin-containing subunit from Pseudomonas syringae pv. tomato str. DC3000
48% identity, 67% coverage
- A computational model of Pseudomonas syringae metabolism unveils a role for branched-chain amino acids in Arabidopsis leaf colonization
Tubergen, PLoS computational biology 2023 - “...in BCAAs biosynthesis. The leucine minimal medium rendered three additional genes predicted to be essential: PSPTO_2736, PSPTO_2738, and PSPTO_2739. The three genes show high sequence homology to the leucine catabolic genes liuD , liuB , and liuA , respectively. 10.1371/journal.pcbi.1011651.g003 Fig 3 iPst19 predicts genes of...”
- “...one iso-propyl-malate dehydrogenase (PSPTO_2175) were essential to metabolize glucose, three genes of the liu operon (PSPTO_2736, PSPTO_2738, and PSPTO_2739) were essential to use leucine as the sole carbon source. (C) Predicted gene essentiality changes with mixed carbon sources where iPst19 gene essentiality was simulated in conditions...”
BMMGA3_RS05255 pyruvate carboxylase from Bacillus methanolicus MGA3
44% identity, 39% coverage
B6SKB7 Methylcrotonoyl-CoA carboxylase subunit alpha from Zea mays
46% identity, 59% coverage
- Leaf Proteomic Analysis in Seedlings of Two Maize Landraces with Different Tolerance to Boron Toxicity
Mamani-Huarcaya, Plants (Basel, Switzerland) 2023 - “...4 FC 3 p -Value 4 FCSA/ FCPA 5 Function/Biological Process 6 AMINO ACID METABOLISM B6SKB7 Zm00001d031013 Methylcrotonoyl-CoA carboxylase subunit 4.44 0.0022 3.56 0.0049 0.80 Leucine degradation A0A1D6K836 Zm00001d029848 Branched-chain amino-acid aminotransferase 2.35 0.0272 1.65 0.0241 0.70 Branched-chain amino acid biosynthesis B4G011 Zm00001d046923 d -3-phosphoglycerate dehydrogenase...”
- “...5 AMINO ACID AND PEPTIDE METABOLISMS Proteins very strongly induced by B toxicity in Pacha B6SKB7 Zm00001d031013 Methylcrotonoyl-CoA carboxylase subunit 4.44 0.0022 Leucine degradation Proteins very strongly repressed by B toxicity in Pacha A0A1D6ICL3 Zm00001d021596 Adenosine 5-phosphosulfate reductase-like1 0.29 0.0140 Cysteine biosynthetic process. Sulfate reduction B6TZD1...”
W5QHK3 Methylcrotonoyl-CoA carboxylase 1 from Ovis aries
43% identity, 65% coverage
PCCA_HALMT / I3R7G3 Propionyl-CoA carboxylase, biotin carboxylase and biotin-carboxyl carrier subunit; PCC; EC 6.4.1.3; EC 6.3.4.14 from Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei) (see paper)
I3R7G3 propionyl-CoA carboxylase (subunit 3/3) (EC 6.4.1.3) from Haloferax mediterranei (see paper)
47% identity, 75% coverage
- function: This is one of the subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), the enzyme catalyzing the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA (By similarity). Propionyl-CoA carboxylase is involved in propionate utilization and in the production of the poly(3-hydroxybutyrate-co-3- hydroxyvalerate)(PHBV), which is a water-insoluble biopolymer used as intracellular energy reserve material when cells grow under conditions of nutrient limitation (PubMed:25398867). Propionyl-CoA carboxylase is also able to catalyze the carboxylation of acetyl-CoA (PubMed:25398867).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
catalytic activity: N(6)-biotinyl-L-lysyl-[protein] + hydrogencarbonate + ATP = N(6)-carboxybiotinyl-L-lysyl-[protein] + ADP + phosphate + H(+) (RHEA:13501)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The propionyl coenzyme A carboxylase (PCC) complex is composed of three subunits: PccA (biotin carboxylase and biotin-carboxyl carrier), PccB (carboxyltransferase) and PccX.
A4HRC6 propionyl-CoA carboxylase from Leishmania infantum
46% identity, 66% coverage
VT47_11875 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Pseudomonas syringae pv. syringae
48% identity, 69% coverage
- Identification of the Genes of the Plant Pathogen Pseudomonas syringae MB03 Required for the Nematicidal Activity Against Caenorhabditis elegans Through an Integrated Approach
Ali, Frontiers in microbiology 2022 - “...stationary phase interaction: lysR transcriptional regulator (VT47_13020), isovaleryl-CoA dehydrogenase (VT47_11890), putative acyl-CoA carboxylase alpha chain (VT47_11875), acyl-CoA carboxyltransferase beta chain (VT47_11885), prpC (VT47_09990), and PrpB (VT47_09985). On the other hand, crfX (VT47_10030), (VT47_19750) and hypothetical protein (VT47_10035) were down regulated ( Table 2 ). Regarding nematicidal...”
- “...h VT47_13020 Yes Unchanged Up LysR family transcriptional regulator |VT47_11890 Yes Unchanged Up Isovaleryl-CoA dehydrogenase VT47_11875 Yes Unchanged Up 3-Methylcrotonyl-CoA carboxylase alpha subunit VT47_11885 Yes Unchanged Up Propionyl-CoA carboxylase prpC VT47_09990 Yes Unchanged Up 2-Methylcitrate synthase prpB VT47_09985 Yes Unchanged Up 2-Methylisocitrate lyase cmpX VT47_10035 Yes...”
Pcca / P14882 propionyl-CoA carboxylase α subunit (EC 6.4.1.3) from Rattus norvegicus (see paper)
P14882 Propionyl-CoA carboxylase alpha chain, mitochondrial from Rattus norvegicus
46% identity, 60% coverage
- Proteomic mapping reveals dysregulated angiogenesis in the cerebral arteries of rats with early-onset hypertension
Bastrup, The Journal of biological chemistry 2023 - “...and casein kinase substrate in neurons 2 protein 0.32 13.94 1.51E-04 1.12E-02 Pcca Isoform of P14882, propanoyl-CoA:carbon dioxide ligase subunit alpha; propionyl-CoA carboxylase alpha chain, mitochondrial 0.20 15.31 5.55E-04 2.53E-02 Pccb Propionyl-CoA carboxylase beta chain, mitochondrial; isoform of P07633, propionyl coenzyme A carboxylase, beta polypeptide 0.26...”
- Extracellular vesicles released by steatotic hepatocytes alter adipocyte metabolism
Mleczko, Journal of extracellular biology 2022 - “...CP2CN_RAT P24470 1.75 8.02E03 Cytochrome P450 2C23 PDIA3_RAT P11598 1.75 5.38E05 Protein disulfideisomerase A3 PCCA_RAT P14882 1.77 1.95E03 PropionylCoA carboxylase alpha chain, mitochondrial ANGL4_RAT Q6TMA8 1.77 3.93E03 Angiopoietinrelated protein 4 HMGCL_RAT P97519 1.78 1.01E03 HydroxymethylglutarylCoA lyase, mitochondrial SDC4_RAT P34901 1.78 1.17E04 Syndecan4 CP2DQ_RAT P10634 1.79 1.03E03...”
- Proteomic analysis of spinal cord tissue in a rat model of cancer-induced bone pain.
Yang, Frontiers in molecular neuroscience 2022 - “...E9PU28 Impdh2 0.13 3.0 Down Q6IFU8 Krt17 0.13 3.0 Down Q6KC51 Ablim2 0.13 3.0 Down P14882 Pcca 0.13 3.0 Down Q5M819 Psph 0.13 3.0 Down Q704S8 Crat 0.13 2.9 Down P63269 Actg2 0.13 2.9 Down P62738 Acta2 0.13 2.9 Down Functional classification of differentially expressed protein...”
- Functional Redox Proteomics Reveal That Salvia miltiorrhiza Aqueous Extract Alleviates Adriamycin-Induced Cardiomyopathy via Inhibiting ROS-Dependent Apoptosis.
Hung, Oxidative medicine and cellular longevity 2020 - “...May play a role in the control of cell proliferation and cellular aging. 3 PCCA P14882 82.198/7.59 113 (32%) 16 Mitochondrion matrix This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty...”
- Functional proteomic analysis of corticosteroid pharmacodynamics in rat liver: Relationship to hepatic stress, signaling, energy regulation, and drug metabolism.
Ayyar, Journal of proteomics 2017 - “...UP P57093 Phyh Phytanoyl-CoA dioxygenase, peroxisomal Fatty acid metabolism - converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA UP P14882 Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial Fatty acid beta oxidation - catalyses the carboxylation reaction of propionyl CoA to form (S)-methylmalonyl CoA UP P70584 Acadsb Short/branched chain acyl-CoA dehydrogenase, mitochondrial...”
- The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats--An Ontogenetic Study
Wille, International journal of molecular sciences 2015 - “...Gmps GMP synthase (glutamine-hydrolyzing) Cp up 0.0062 - - 0.52 5 130 17 77,507 6.21 P14882 PCCA_RAT Pcca Propionyl-CoA carboxylase alpha chain, mitochondrial Mmt down 0.0283 - - 2.14 5 262 32 82,198 7.59 Fat Metabolism Q5XI22 THIC_RAT Acat2 Acetyl-CoA acetyltransferase, cytosolic Cp - - up...”
- Identification of εPKC targets during cardiac ischemic injury
Budas, Circulation journal : official journal of the Japanese Circulation Society 2012 - “...225 83kDa 6.18 75kDa 6.35 5.1 mitochondria 0.02* 8 Propionyl-CoA carboxylase alpha chain, mitochondrial precursor P14882 12 78 77kDa 6.33 69kDa 6.41 5.1 mitochondria 0.02* 9 Dihydrolipoyl dehydrogenase, mitochondrial precursor Q6P6R2 13 207 54kDa 7.96 48kDa 7.33 4.1 mitochondria 0.01* 10 ATP synthase subunit alpha, mitochondrial...”
- Use of biotinylated ubiquitin for analysis of rat brain mitochondrial proteome and interactome.
Buneeva, International journal of molecular sciences 2012 - “...beta chain, mitochondrial precursor P07633 13 67.94 M 2 Propionyl-CoA carboxylase alpha chain, mitochondrial precursor P14882 5 37.33 M Transporters ( n = 4) 1 Excitatory amino acid transporter 1 P24942 6 34.57 PM 2 Excitatory amino acid transporter 2 P31596 2 26.35 PM 3 Glial...”
- “...P26284, Dlat = P08461, Hspd1 = P63039, Hspe1 = P26772, Slc25a5 = Q09073, Pcca = P14882, Pccb = P07633, Atp1a2 = P06686, Atp1a1 = P06685, Nefm = P12839, Nefl = P19527, Camk2a = P11275, Ina = P23565, Gfap = P47819, Slc1a3 = P24942, ENSRNOG00000018630 = Q0QEU1,...”
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BR1781 pyruvate carboxylase from Brucella suis 1330
47% identity, 38% coverage
- RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant of In vivo Brucella suis Pathogenicity
Abdou, Frontiers in cellular and infection microbiology 2017 - “...quantitative PCR (RT-qPCR) (Table S3 ). Only seven genes were not validated, because of opposite (BR1781 and BR1043) or below threshold (1.5) (BR0111, BR1017, BR1358, BR1648, and BRA0066) fold-change values. It has to be noticed that BR1358 ( ureC-2 ) and BRA0066 ( virB4 ) belong...”
- “...tested (underlined gray) are shown. The red box represents the only gene up-regulated by RegA. BR1781 ( pyc ) and BR1017 ( maeB ), not validated, were omitted for clarity. (C) ICL activity in crude lysates of the wild-type (WT) (dark gray bar), regA mutant (...”
8xl6A / Q96RQ3 Structure of human 3-methylcrotonyl-coa carboxylase at apo-state (mcc- apo)
44% identity, 69% coverage
CAETHG_1594 pyruvate carboxylase from Clostridium autoethanogenum DSM 10061
44% identity, 39% coverage
- Clostridium autoethanogenum alters cofactor synthesis, redox metabolism, and lysine-acetylation in response to elevated H2:CO feedstock ratios for enhancing carbon capture efficiency
Davin, Biotechnology for biofuels and bioproducts 2024 - “...key proteins, the biotin-carrier component of acetyl-CoA carboxylase complex (ACC, CAETHG_0127) and pyruvate carboxylase (PYC, CAETHG_1594), are regulated post-translationally by BirA biotinylation in the related bacterium, C. ljungdahlii [ 26 ]. This report prompted an examination of biotinylation in C. autoethanogenum . The search for biotinylation...”
- Comparison of single-molecule sequencing and hybrid approaches for finishing the genome of Clostridium autoethanogenum and analysis of CRISPR systems in industrial relevant Clostridia
Brown, Biotechnology for biofuels 2014 - “...dikinase (PPDK) CAETHG_2055, 2909, P 3 Pyruvate kinase (Pk) CAETHG_2440-41, P 4 Pyruvate carboxylase (Pyc) CAETHG_1594, P 5 PEP carboxykinase (PEPCK) CAETHG_2721, P 5 Malic enzyme CAETHG_0605, 1055. Incomplete TCA cycle. T 1 Citrate synthase CAETHG _2751, T 2 Citrate lyase CAETHG_1052-54, 18981901, 2480-83, T 3...”
SCO6271 acyl-CoA carboxylase complex A subunit from Streptomyces coelicolor A3(2)
Q9EWV4 biotin carboxylase from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
SCO4921 acyl-CoA carboxylase complex A subunit from Streptomyces coelicolor A3(2)
49% identity, 72% coverage
- Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics
Dulermo, Microorganisms 2023 - “...of the nine genes of S. coelicolor M145 encoding putative acyl/acetyl/propionyl CoA carboxylases, sco4921 and sco6271 , were deleted in the TD variant. These enzymes, that catalyze the first step of fatty acid biosynthesis, are usually constituted by two subunits: and . In S. coelicolor ,...”
- “...well as SCO4921 (AccA2, acyl-CoA carboxylase subunit ), SCO4926 (PccB, propionyl-CoA carboxylase subunit ) and SCO6271 (Acc1, acyl-CoA carboxylase subunit ). AcetylCoA carboxylase catalyzes the carboxylation of acetyl-CoA to produce malonyl-CoA yielding straight/iso fatty acids. Propionyl CoA carboxylase catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA yielding...”
- Dissection of 3D chromosome organization in Streptomyces coelicolor A3(2) leads to biosynthetic gene cluster overexpression
Deng, Proceedings of the National Academy of Sciences of the United States of America 2023 - “...the one of 4.3 Mb. Interestingly, this region comprised three precursor supply genes (SCO6269 to SCO6271) for the synthesis of malonyl-CoA ( 51 ), the assembly unit for the polyketide synthase (PKS), and well-characterized regulation genes (SCO6265 to SCO6266) for -butyrolactone (GBL) signaling circuits, which hints...”
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...CpkL (SCO6285) were the only members of the cpk cluster which were not detected. AccA1 (SCO6271) differs by only four amino acids from AccA2 (SCO4921), therefore it is not possible to distinguish accurately between these two proteins by the shotgun proteomics method. Almost all detected peptides...”
- “...biotin as a cofactor. In S. coelicolor A3(2) there are two almost identical subunits, AccA1 (SCO6271), encoded within cpk gene cluster, and essential AccA2 (SCO4921) ( Rodrguez et al., 2001 ). We have found that ACCase subunit(s) AccA2 and/or AccA1, subunits AccB (SCO5535) and CpkK (SCO6284),...”
- AccR, a TetR Family Transcriptional Repressor, Coordinates Short-Chain Acyl Coenzyme A Homeostasis in Streptomyces avermitilis
Lyu, Applied and environmental microbiology 2020 (secret) - Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor
Nitta, Frontiers in bioengineering and biotechnology 2020 - “...This observation also matches the high levels of expression of the two acetyl-CoA carboxylaseencoding genes, SCO6271 ( accA1 ) and SCO4921 ( accA2 ) in M1146 + ACT ( Figure 4B ). When malonyl-CoA levels were analyzed by LC-MS/MS, malonyl-CoA decreased with time, and the malonyl-CoA...”
- ScbR- and ScbR2-mediated signal transduction networks coordinate complex physiological responses in Streptomyces coelicolor
Li, Scientific reports 2015 - “...In addition to kasO and sco6268 promoters, it also bound at the intergenic regions of sco6271 - sco6272 , sco6275 - 6276 , and sco6282 - sco6283 , and the promoter of another regulatory gene sco6288 . Similarly, in the cda cluster, beside the CSR gene...”
- Crp is a global regulator of antibiotic production in streptomyces
Gao, mBio 2012 - “...pathway-specific activator; Red synthesis 590 G T CTCGCCCAC C A C (520) SCO6271-SCO6272 i 1.83 SCO6271 AccA1, acyl-CoA carboxylase complex; yCPK synthesis 51 G T G A GGAGAA T C TT (8) SCO6272 Scf, secreted FAD j -binding protein, yCPK synthesis 257 AA G A TTCTCC...”
- “...synthesis SCO5221-5222 SCO5222 Repressed EizA, putative lyase SCO5223 SCO5223 Repressed Putative cytochrome P450 SCO6271-6272 c SCO6271 Activated AccA1, acyl-CoA carboxylase complex; yCPK synthesis SCO6272 Activated Scf, secreted FAD d -binding protein; yCPK synthesis SCO6272 c SCO6272 Activated Scf, secreted FAD-binding protein; yCPK synthesis SCO6275-6276 c SCO6275...”
- Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
Thomas, Molecular & cellular proteomics : MCP 2012 - “...led to increased expression of Act (42, 43). SCO6271 (AccA1), SCO4921 (AccA2), and SCO5535 (AccB) encode subunits of an acetyl-CoA carboxylase (44). SCO4921 and...”
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- High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties.
Menendez-Bravo, Biotechnology for biofuels 2016 - “...S. coelicolor A3 (2) SCO4921 Q9X4K7 pccB, propionyl-CoA carboxylase subunit S. coelicolor A3 (2) SCO4926 Q9EWV4 pccE , propionyl-CoA carboxylase subunit S. coelicolor A3 (2) SCO4925 Q9EWV8 sfp , phosphopantetheinyl transferase B. subtilis BSU03570 X63158 prpE , propionyl-CoA synthetase E. coli b0335 P77495 pMB20 was constructed...”
- σE of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
Pospíšil, Communications biology 2024 - “...B (HrdB-dependent, Fig. 5 ), we tested SCO1595- pheS , SCO5393, SCO5535- accB , and SCO4921- accA2 as genes likely positively regulated by HrdB. Here, the selection was made based on the presence of predicted HrdB binding sequences upstream of the genes. Except for SCO5393, all...”
- “...be regulated by HrdB instead of E (SCO1595- pheS , SCO5393, SCO5535- accB , and SCO4921- accA2 ). The Figure description is the same as in Fig. 4 . The blue rectangles show the -10 region of HrdB-dependent promoters (if identified in ref. 4 ). The...”
- Genome Analysis of a Variant of Streptomyces coelicolor M145 with High Lipid Content and Poor Ability to Synthetize Antibiotics
Dulermo, Microorganisms 2023 - “...Biosynthesis Two of the nine genes of S. coelicolor M145 encoding putative acyl/acetyl/propionyl CoA carboxylases, sco4921 and sco6271 , were deleted in the TD variant. These enzymes, that catalyze the first step of fatty acid biosynthesis, are usually constituted by two subunits: and . In S....”
- “...catalyzes the carboxylation of propionyl-CoA to methylmalonyl-CoA yielding branched/ante-iso fatty acids ( Figure 5 ). SCO4921 and SCO6271 might constitute the and subunits of a carboxylase complex that would be absent in the TD variant. This leaves six genes encoding three functional carboxylase complexes, SCO2445/44, SCO2776/77...”
- Coelimycin Synthesis Activatory Proteins Are Key Regulators of Specialized Metabolism and Precursor Flux in Streptomyces coelicolor A3(2)
Bednarz, Frontiers in microbiology 2021 - “...cluster which were not detected. AccA1 (SCO6271) differs by only four amino acids from AccA2 (SCO4921), therefore it is not possible to distinguish accurately between these two proteins by the shotgun proteomics method. Almost all detected peptides can be attributed to both of them. AccA1 and/or...”
- “...are two almost identical subunits, AccA1 (SCO6271), encoded within cpk gene cluster, and essential AccA2 (SCO4921) ( Rodrguez et al., 2001 ). We have found that ACCase subunit(s) AccA2 and/or AccA1, subunits AccB (SCO5535) and CpkK (SCO6284), and a small accessory protein AccE (SCO5536), were more...”
- The Onset of Tacrolimus Biosynthesis in Streptomyces tsukubaensis Is Dependent on the Intracellular Redox Status
Pires, Antibiotics (Basel, Switzerland) 2020 - “...STSU_10154 SCO5374 ATP synthase subunit epsilon (AtpC) 0.42 STSU_11515 SCO5044 Fumarate hydratase (FumB) 0.38 STSU_12400 SCO4921 putative acyl-CoA carboxylase complex A subunit 0.09 STSU_12680 SCO4855 succinate dehydrogenase iron-sulfur subunit (SdhB) 0.50 1 STSU_30056 SCO1391 Phosphoenolpyruvate-protein phosphotransferase (EI component) 0.45 Amino acid metabolism STSU_14552 SCO3345 Dihydroxy-acid dehydratase...”
- Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus
Lee, The ISME journal 2020 - “...the acetyl-CoA synthetase (SCO6195) converted acetate to acetyl-CoA; and (iii) acetyl-CoA carboxylases (SCO2776, SCO2777, and SCO4921) converted acetyl-CoA to malonyl-CoA (Fig. 1e ). Considering that the transcription levels of genes involved in other acetyl-CoA-associated pathways, such as glycolysis and the citrate cycle, showed no differences between...”
- Multi-Omics Analysis of the Effect of cAMP on Actinorhodin Production in Streptomyces coelicolor
Nitta, Frontiers in bioengineering and biotechnology 2020 - “...high levels of expression of the two acetyl-CoA carboxylaseencoding genes, SCO6271 ( accA1 ) and SCO4921 ( accA2 ) in M1146 + ACT ( Figure 4B ). When malonyl-CoA levels were analyzed by LC-MS/MS, malonyl-CoA decreased with time, and the malonyl-CoA level was lower in M1146...”
- De novo biosynthesis of myricetin, kaempferol and quercetin in Streptomyces albus and Streptomyces coelicolor
Marín, PloS one 2018 - “.... coelicolor chromosomal genes birA (biotin ligase, SCO4927 gene), accA2 (alpha subunit of acetyl-CoA carboxylase, SCO4921 gene) and accBE (beta and epsilon subunits of acetyl-CoA carboxylase, SCO5535 and SCO5536 genes respectively) [ 49 51 ]. These plasmids were amplified by PCR from S . coelicolor chromosomal...”
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MCCC1 / Q96RQ3 3-methylcrotonyl-CoA carboxylase α subunit (EC 6.4.1.4) from Homo sapiens (see 4 papers)
MCCA_HUMAN / Q96RQ3 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 from Homo sapiens (Human) (see 15 papers)
44% identity, 63% coverage
- function: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
catalytic activity: 3-methyl-(2E)-butenoyl-CoA + hydrogencarbonate + ATP = 3- methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+) (RHEA:13589)
cofactor: biotin
subunit: Probably a dodecamer composed of six biotin-containing alpha subunits (MCCC1) and six beta (MCCC2) subunits (PubMed:17360195). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). - Aqueous Extracts of Ocimum gratissimum Sensitize Hepatocellular Carcinoma Cells to Cisplatin through BRCA1 Inhibition.
Chen, International journal of molecular sciences 2024 - “...ITPRIPL2 Inositol 1,4,5-trisphosphate receptor-interacting protein-like 2 0.01 0.01 Q9BRS8 LARP6 La-related protein 6 0.01 0.01 Q96RQ3 MCCC1 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial 0.01 0.01 Q99081 TCF12 Transcription factor 12 0.01 0.01 Q13796 SHROOM2 Protein Shroom2 0.01 0.01 Q69YH5 CDCA2 Cell division cycle-associated protein 2 0.01 0.01...”
- SAV-Pred: A Freely Available Web Application for the Prediction of Pathogenic Amino Acid Substitutions for Monogenic Hereditary Diseases Studied in Newborn Screening.
Zadorozhny, International journal of molecular sciences 2023 - “...5 58 208 271 21 15 0.703 0.707 HADHA Long-chain L-3 hydroxyacyl-CoA dehydrogenase deficiency 600890 Q96RQ3 12 9 476 497 9 11 0.813 0.808 HADHB Trifunctional protein deficiency 143450 P50747-1 2 14 309 325 17 5 0.961 0.961 HBB Hemoglobinopathies 141900 P68871 27 149 79 255...”
- Interactome Analysis of the ER Stress Sensor Perk Uncovers Key Components of ER-Mitochondria Contact Sites and Ca2+ Signalling
Sassano, Contact (Thousand Oaks (Ventura County, Calif.)) 2021 - “...27 17 100% 100% Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial OS=Homo sapiens OX=9606 GN=MCCC1 PE=1 SV=3 Q96RQ3 MCCC1 80 kDa 50 25 40 19 100% 100% Lysine-rich nucleolar protein 1 OS=Homo sapiens OX=9606 GN=KNOP1 PE=1 SV=1 Q1ED39 KNOP1 52 kDa 61 33 42 23 100% 100% Arginine...”
- Inhibition of Mitochondrial Complex Function-The Hepatotoxicity Mechanism of Emodin Based on Quantitative Proteomic Analyses
Lin, Cells 2019 - “...Process GO:0005759 mitochondrial matrix 506 30 1.5 10 16 5.7 10 16 O76031, P48735, Q9BQC6, Q96RQ3, Q9UNQ2, Q07820, Q9NYK5, Q32P41, Q6NVY1, Q8N983, Q9BZE1, Q9H2W6, Q16822, Q13084, O95182, P30038, Q8IXM3, Q969S9, Q9Y291, P11498, Q5U5X0, Q8NFF5, P82932, O00217, P82675, P28331, O75306, P42704, Q96AG4, Q13057 Cellular Component GO:0005761 mitochondrial...”
- Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls.
Berge, Clinical proteomics 2019 - “...0.87 P20645 Cation-dependent mannose-6-phosphate receptor M6PR 0.006115 0.1934 22.3302 0.22866 23.6924 0.17682 22 4 0.84 Q96RQ3 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial MCCC1 0.007633 0.5028 21.2898 0.19278 23.5502 0.34081 16.4 7 0.83 P78417 Glutathione S-transferase omega-1 GSTO1 0.007795 0.2279 23.6102 0.09562 23.1469 0.19987 59.3 14 0.79 P24666...”
- Identification of proteins interacting with the mitochondrial small heat shock protein Hsp22 of Drosophila melanogaster: Implication in mitochondrial homeostasis
Dabbaghizadeh, PloS one 2018 - “...Phosphatidyl glycerophosphatase and protein-tyrosine phosphatase1 (Q8WUK0) 0.33 0.1 0 0 Methylcrotonyl-CoA carboxylase subunit alpha, mitochondrial (Q96RQ3) 0 0 0.34 0 Isoform 3 of Carbamoyl-phosphate synthase, mitochondrial (P31327) 0 0 0.74 0.04 Pyruvate carboxylase, mitochondrial (P11498) 0 0.04 1 0.1 0.06 Hydroxysteroid dehydrogenase-like protein 2 (Q6YN16) 0.16...”
- SAMHD1 Posttranscriptionally Controls the Expression of Foxp3 and Helios in Human T Regulatory Cells.
Kim, Journal of immunology (Baltimore, Md. : 1950) 2018 - “...MCCB_HUMAN 348.2093251 (Q9HCC0) Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial Mitochondrial enzymes with Biotin cofactor MCCA_HUMAN 249.9351293 (Q96RQ3) Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Mitochondrial enzymes with Biotin cofactor PCCA_HUMAN 93.580363 (P05165) Propionyl-CoA carboxylase alpha chain, mitochondrial Mitochondrial enzymes with Biotin cofactor DLDH_HUMAN 165.104467 (P09622) Dihydrolipoyldehydrogenase, mitochondrial Photo damage-related...”
- Mitochondrial O-GlcNAc Transferase (mOGT) Regulates Mitochondrial Structure, Function, and Survival in HeLa Cells.
Sacoman, The Journal of biological chemistry 2017 - More
D0N1Q9 Methylcrotonoyl-CoA carboxylase subunit alpha, putative from Phytophthora infestans (strain T30-4)
45% identity, 65% coverage
- Cinnamaldehyde inhibits the growth of Phytophthora capsici through disturbing metabolic homoeostasis
Wang, PeerJ 2021 - “...Down/0.48 H3GJ13 1,3-beta-glucanosyltransferase Down/0.81 H6U2P7 Cellulose synthase 3 Down/0.70 Leucine metabolism A0A0W8CX17 Isovaleryl-CoA dehydrogenase Down/0.63 D0N1Q9 Methylcrotonoyl-CoA carboxylase 1 Down/0.77 Discussion There are few reports on the mechanism of action of CA on fungi and oomycetes. OuYang et al. (2019) found that after CA treatment, the...”
- “...metabolic W2NUF9 CAMK/CAMK1 protein kinase Down/0.319 D0NCV1 Glucan 1,3-beta-glucosidase Down/0.408 H3GJ13 1,3-beta-glucanosyltransferase Down/0.491 Leucine metabolism D0N1Q9 Methylcrotonoyl-CoA carboxylase subunit alpha, putative Down/0.453 A0A0W8CX17 Isovaleryl-CoA dehydrogenase Down/0.345 Internal reference protein A0A0W8DRZ2 40S ribosomal protein S13 Q2M3Z6 Ribosomal protein L23 10.7717/peerj.11339/fig-8 Figure 8 Relative expression of mRNAs from...”
CLJU_c37390 pyruvate carboxylase from Clostridium ljungdahlii DSM 13528
44% identity, 39% coverage
- Pleiotropic Regulator GssR Positively Regulates Autotrophic Growth of Gas-Fermenting Clostridium ljungdahlii
Zhang, Microorganisms 2023 - “...1 , No. 615) to examine phenotypic outcomes. Among these candidates, only the overexpression of CLJU_c37390 significantly affected the autotrophic growth of C. ljungdahlii ( Figure 4 A), while the others had no influence on cell growth ( Supplementary Figure S2 ). According to the genome...”
- “...38 ]. Based on this finding, we further examined the interaction between GssR and the CLJU_c37390 gene using EMSA. Specifically, a 305 bp DNA fragment covering the promoter region of the CLJU_c37390 gene was used as the DNA probe. As shown in Figure 4 B, GssR...”
- Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas-fermenting Clostridium ljungdahlii
Zhang, Microbial biotechnology 2021 - “...Nterminal DNAbinding domain of Cl BirA) (Fig. 8A ); simultaneously, the acc (Clju_c4210042140) and pyc (Clju_c37390) genes, coding for the C. ljungdahlii ACC and PYC enzymes, respectively, were also overexpressed separately (Fig. 8A ). Such an engineering strategy was expected to specifically block the regulatory function,...”
- Clostridium ljungdahlii represents a microbial production platform based on syngas
Köpke, Proceedings of the National Academy of Sciences of the United States of America 2010 - “...and C4 compounds: pyruvate carboxylase (encoded by CLJU_c37390) and PEP-carboxykinase (encoded by CLJU_06210). C. ljungdahlii operates a branched TCA "cycle,"...”
ATE51_00984 acetyl-CoA carboxylase biotin carboxylase subunit from Campylobacter coli
45% identity, 96% coverage
- Characterisation of Aerotolerant Forms of a Robust Chicken Colonizing Campylobacter coli
O'Kane, Frontiers in microbiology 2017 - “...fixed with 100% 9 Cs at this location. This position is within genes ATE51_00982 and ATE51_00984. In the 10 C configuration, the reading frame ATE51_00982 encodes a 593 amino acid protein that shares high sequence identity with PseD proteins (97 to 100% identity) from many other...”
- “...acid residue and protein termination at 21 amino acids. The 10 C configuration of gene ATE51_00984 encodes a 60 amino acid protein. However, the 9 C configuration results in a frame shift after 60 amino acid residues and fusion with ATE51_00982 resulting in a 648 amino...”
LA_2736 acetyl-CoA carboxylase biotin carboxylase subunit from Leptospira interrogans serovar lai str. 56601
45% identity, 91% coverage
MCCA_ARATH / Q42523 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
Q42523 methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) from Arabidopsis thaliana (see 2 papers)
NP_849583 methylcrotonyl-CoA carboxylase alpha chain from Arabidopsis thaliana
AT1G03090 MCCA; methylcrotonoyl-CoA carboxylase from Arabidopsis thaliana
45% identity, 60% coverage
- function: Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.
catalytic activity: 3-methyl-(2E)-butenoyl-CoA + hydrogencarbonate + ATP = 3- methyl-(2E)-glutaconyl-CoA + ADP + phosphate + H(+) (RHEA:13589)
cofactor: biotin
cofactor: Mn(2+) (Binds 2 manganese ions per subunit.)
subunit: Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits. - Genetic dissection of methylcrotonyl CoA carboxylase indicates a complex role for mitochondrial leucine catabolism during seed development and germination.
Ding, The Plant journal : for cell and molecular biology 2012 (PubMed)- GeneRIF: Studies indicate that mutations in either 3-methylcrotonyl CoA carboxylase MCCA (At1g03090) or MCCB (At4g34030) block mitochondrial Leu catabolism, as inferred from the increased accumulation of Leu.
- Changes in annual transcriptome dynamics of a clone of Japanese cedar (Cryptomeria japonica D. Don) planted under different climate conditions
Nose, PloS one 2023 - “...- - - Uncharacterised conserved protein (UCP012943) temp-30d -0.76 1.14E-15 CUST_6388_PI429951308 reCj20995:-SWR:isotig20847 8.75E-17 4.82E-14 2.61E-17 AT1G03090 0.0 MCCA methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 temp-0h -0.76 1.15E-15 CUST_3521_PI429951308 reCj18106:--WR:isotig17958 1.90E-15 3.21E-14 3.30E-16 AT5G45140 0.0 NRPC2 nuclear RNA polymerase C2 temp-0h -0.76 1.34E-15 CUST_11766_PI429951308...”
- Cadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis
Gibbs, The Plant journal : for cell and molecular biology 2021 - “...in the EMBL/GenBank data libraries under accession numbers: BIO3BIO1 (AT5G57590), BIO2 (AT2G43360), BIO4 (AT5G04620), MCCA (AT1G03090), BCCP1 (AT5G16390), PAO4 (AT1G65840), OCT1 (AT1G73220), PP2A (AT1G69960), and RPT4A (AT5G43010). AUTHOR CONTRIBUTIONS NMG, HAM, and PHM designed the research; NMG, SHS, SLN, SM, and CA performed the research; NMG,...”
- Network Topological Analysis for the Identification of Novel Hubs in Plant Nutrition
Di, Frontiers in plant science 2021 - “...helicase A0A0A0L0B9 Csa_4G082380 MBL1 (AT1G78850) 50% 434 3,9 2,1E-02 39 Mannose-binding lectin A0A0A0K5L0 Csa_7G387180 MCCA (AT1G03090) 69% 1023 38 4 3 10 Methyl crotonyl-CoA carboxylase subunit alpha A0A0A0KJ29 Csa_6G526470 AT3G58140 74% 657 36 Phenylalanyl-tRNA synthetase A0A0A0KCX8 Csa_6G077980 ARGAH1 (AT4G08900) 85% 590 34 1 0 4 Arginase...”
- The function of glutaredoxin GRXS15 is required for lipoyl-dependent dehydrogenases in mitochondria
Moseler, Plant physiology 2021 - “...replicates using the program GraphPad Prism version 6. Accession numbers GRXS15 (At3g15660), BIO2 (At2g43360), MCCA (At1g03090), ATM3 (At5g58270), CNX2 (At2g31955), CNX3 (At1g01290), NR1 (At1g77760), NR2 (At1g37130), AAO1 (At5g20960), AAO2 (At3g43600), AAO3 (At2g27150), XDH1 (At4g34890) XDH2 (At4g34900), ACO1 (At4g35830), ACO2 (At4g26970), ACO3 (At2g05710), mLIP1 (At2g20860), IVDH (At3g45300),...”
- What Antarctic Plants Can Tell Us about Climate Changes: Temperature as a Driver for Metabolic Reprogramming
Bertini, Biomolecules 2021 - “...AMINOPEPTIDASE 1) 9.74 10 4 1.60 AT5G58070 ATTIL, TIL, temperature-induced lipocalin 5.70 10 3 1.40 AT1G03090 MCCA, methylcrotonyl-CoA carboxylase alpha chain, mitochondrial/3-methylcrotonyl-CoA carboxylase 1 (MCCA) 7.70 10 3 1.28 AT1G54050 HSP20-like chaperones superfamily protein 2.25 10 3 0.94 AT2G22780 PMDH1, peroxisomal NAD-malate dehydrogenase 1 6.05 10...”
- Identification of QTNs and Their Candidate Genes for 100-Seed Weight in Soybean (Glycine max L.) Using Multi-Locus Genome-Wide Association Studies
Ikram, Genes 2020 - “...Glyma10g36070 underlying the stable QTNs qcHSW-10-1 and qcHSW-10-4 were homologous to, respectively, the Arabidopsis genes AT1G03090 ( MCCA ) and AT1G35680 ( RPL21 ), which are related to seed weight or development ( Table 5 ; Figure 4 and Figure 5 ). 4. Discussion To dissect...”
- “...BR-signaling kinase 3 Plant hormone signal transduction [ 67 ] qcHSW-10-1 Gm10_2563422-2566365 Glyma10g03440 Gm10:2482369-2489108 MCCA AT1G03090 methylcrotonyl-CoA carboxylase alpha chain Metabolic pathways [ 68 ] qcHSW-10-4 Gm10_44349893-44469282 Glyma10g36070 Gm10:44258213-44261525 RPL21 AT1G35680 Ribosomal protein L21 Ribosome [ 69 ] The bold text candidate genes were previously reported...”
- Identification of small RNAs during cold acclimation in Arabidopsis thaliana
Tiwari, BMC plant biology 2020 - “...Plant Tudor-like RNA-binding protein 2.17 AT3G11830 TCP-1/cpn60 chaperonin family protein 2.86 AT3G11840 Plant U-box24 2.9 AT1G03090 Methylcrotonyl-CoA carboxylase alpha chain, mitochondrial / 3-methylcrotonyl-CoA carboxylase 1 (MCCA) 2.5 AT1G03100 Pentatricopeptide repeat (PPR) superfamily protein 2.47 AT1G72030 Acyl-CoA N-acyltransferases (NAT) superfamily protein 2.3 AT1G72040 Deoxyribonucleoside kinase 2.44 AT2G40420...”
- “...family protein (AT3G11830) and plant U-box24 (AT3G11840) and PPR (AT1G03100) pairing with mitochondrial/3-methylcrotonyl-CoA carboxylase 1 (AT1G03090). The inverse expression pattern of these pairing transcripts was accompanied by the induction of cis -nat-siRNAs in cold treatment. An ideal example is represented by the cold responsive upregulation of...”
- Transcript and metabolite changes during the early phase of abscisic acid-mediated induction of crassulacean acid metabolism in Talinum triangulare
Maleckova, Journal of experimental botany 2019 - “...CARB 1.06 5 KMT12470 AT1G64660 MGL 2.67 5 KMT14300 AT1G55510 BCDH BETA1 1.50 6 KMT10213 AT1G03090 MCCA 1.57 6 KMT12470 AT1G64660 MGL 2.67 5 KMS98028 AT1G08630 THA1 1.49 5 Solute transport KMT02985 AT2G26900 BASS2 1.05 6 KMT13799 AT1G08960 CAX11 1.02 6 KMT03912 AT1G30360 ERD4 1.60 5...”
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CVAR_2091 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium variabile DSM 44702
47% identity, 65% coverage
FRAAL3159 Acetyl-/propionyl-coenzyme A carboxylase alpha chain [Includes: Biotin carboxylase and Biotin carboxyl carrier protein (BCCP)] from Frankia alni ACN14a
54% identity, 65% coverage
- The Proteogenome of Symbiotic Frankia alni in Alnus glutinosa Nodules
Pujic, Microorganisms 2022 - “...acyl-dehydrogenases (FRAAL1659-FRAAL1670) were not identified. Almost all carboxylases were underabundant such as three acetyl-CoA carboxylases (FRAAL3159, FC = 0.55; FRAAL1213, FC = 0.43; FRAAL3158, FC = 0.23), a methyl-crotonoyl-CoA carboxylase (FRAAL2343, FC = 0.15) and two propionyl-CoA carboxylases (FRAAL5672, FC = 0.05; FRAAL1210, FC = 0.68)....”
AO271_00235 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium diphtheriae
48% identity, 72% coverage
SM_b21124 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) from Sinorhizobium meliloti 1021
SMb21124 putative methylcrotonoyl-CoA carboxylase biotinylated subunit protein from Sinorhizobium meliloti 1021
50% identity, 68% coverage
O25134 Biotin carboxylase from Helicobacter pylori (strain ATCC 700392 / 26695)
HP0370 biotin carboxylase (accC) from Helicobacter pylori 26695
45% identity, 96% coverage
- Outer Membrane Vesicles Secreted by Helicobacter pylori Transmitting Gastric Pathogenic Virulence Factors
Wei, ACS omega 2022 - “...P56109 fba 94 O25825 HP_1227 95 O25856 NQO3 96 O34523 Omp29 97 O25927 lpxA 98 O25134 HP_0370 99 O25399 HP_0690 100 P56029 rplA 101 O25738 HP_1110 102 O25414 HP_0710 103 O25423 HP_0721 104 O25347 HP_0630 105 O25715 HP_1083 106 P56456 ileS 107 P56145 pheT 108 P56031...”
- “...P56006 scoA 191 O25140 DsbC 192 O25926 clpX 193 P56109 fba 194 P56460 metK 195 O25134 HP_0370 196 O25213 HP_0466 197 Q48248 cdh 198 P56127 metG 199 P64655 HP_0135 200 P56052 rpmC 201 O26091 rlpA 202 P56031 rplC 203 Q09066 ureG 204 O25018 HP_0232 205 O25902...”
- ModBase, a database of annotated comparative protein structure models, and associated resources
Pieper, Nucleic acids research 2011 - “...illustrate the use of these models with the enzyme biotin carboxylase (locus HP_0370, UniProt accession O25134, gi 2313468). Biotin carboxylase catalyzes an early step in fatty acid biosynthesis. Thus, bacterial biotin carboxylases are investigated as potential drug targets using virtual screening ( 53 ). Because these...”
- A Survey of Helicobacter pylori Antibiotic-Resistant Genotypes and Strain Lineages by Whole-Genome Sequencing in China
Zhou, Antimicrobial agents and chemotherapy 2022 - “...In addition, we investigated previously reported genetic mutations associated with metronidazole resistance, including RclC , HP0370 , HP0918 , Rpsu , Fur , RecA , Ribf , and Omp11 ( 29 , 35 38 ), and found that only V265I of HP0370 and A51V/T of HP0918...”
- “...with metronidazole resistance, such as mutations in frxA , rpsu , RclC , HP0918 , HP0370 , and other genes, and no meaningful results were found. This means that the inactivation of rdxA function is responsible for the development of resistance to metronidazole in some strains....”
- Analysis of core protein clusters identifies candidate variable sites conferring metronidazole resistance in Helicobacter pylori
Chua, Gastroenterology report 2019 - “...in RdxA, D85N in the inner-membrane protein RclC (HP0565), V265I in a biotin carboxylase protein (HP0370) and A51V/T in a putative threonylcarbamoylAMP synthase (HP0918). Conclusions Our approach identified new potential mechanisms for metronidazole resistance in H. pylori that merit further investigation. Helicobacter pylori metronidazole antibiotic resistance...”
- “...protein RclC (HP0565) 978 V265 35 47 12 (I) 1 (I) 66 0.047 Biotin carboxylase (HP0370) 993 A51 33 47 9 (V), 5 (T) 1 (V) 41 0.006 Putative threonylcarbamoylAMP synthase (HP0918) AA, amino acid; MR, metronidazole-resistant; MS, metronidazole-susceptible. a Alignment of each protein cluster is...”
- Carbon Fixation Driven by Molecular Hydrogen Results in Chemolithoautotrophically Enhanced Growth of Helicobacter pylori
Kuhns, Journal of bacteriology 2016 - “...3.0 3.0 2.9 2.9 2.9 Soluble proteins HP0515 HP1317 HP0370 HP1152 HP1460 HP1430 HP0163 HP1458 HP0466 HP0306 HP0138 HP0088 HP0742 HP1182 Heat shock protein (HslV)...”
- “...biosynthesis via two half-reactions. Biotin carboxylase (AccC, or HP0370 [7]) is one component of ACC and catalyzes the ATP-dependent carboxylation of biotin in...”
- Global analysis of Helicobacter pylori gene expression in human gastric mucosa
Graham, Gastroenterology 2002 - “...HP0114 HP0343 b HP0685 HP1144 HP1475 JHP0917 HP0119 HP0370 HP0725 HP1176 HP1477 JHP0921 HP0120 c HP0441 c HP0765 HP1187 HP1528 JHP0936 HP0146 HP0454 HP0767...”
- Acid-induced gene expression in Helicobacter pylori: study in genomic scale by microarray
Ang, Infection and immunity 2001 - “...0 0 11,995 5,219 0 14,166 0 4,465 HP0286 HP0370 HP0480 HP0123 HP0099 HP0183 HP0407 HP1139 HP0634 HP0572-2 HP1372 HP0632 HP1290 HP1448 Cell division protein...”
- Metabolism and genetics of Helicobacter pylori: the genome era
Marais, Microbiology and molecular biology reviews : MMBR 1999 - “...and the genes encoding the proteins in the complex, HP0370 (accC), HP0371 (fabE), HP0557 (accA), and HP0950 (accD), have been found in H. pylori DNA. The next...”
G3I4L6 Pyruvate carboxylase from Cricetulus griseus
46% identity, 38% coverage
Q5I0C3 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial from Rattus norvegicus
45% identity, 61% coverage
SACE_0028 acetyl-CoA carboxylase from Saccharopolyspora erythraea NRRL 2338
51% identity, 92% coverage
Q3TCQ3 Pyruvate carboxylase from Mus musculus
46% identity, 38% coverage
AOT42_01480, DIP0649 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Corynebacterium diphtheriae bv. gravis
NP_939023 acyl coenzyme A carboxylase from Corynebacterium diphtheriae NCTC 13129
48% identity, 72% coverage
F1LP30 Methylcrotonyl-CoA carboxylase subunit 1 from Rattus norvegicus
45% identity, 58% coverage
- Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome
Barker, The Biochemical journal 2024 - “...(Q5U2P5) Hspa1a (P0DMW1) Acaca * (P11497) Sidt2* (D3ZEH5) Snap25* (P60881-2) Snx3* (Q5U211) Myh11 (E9PTU4) Mccc1 (F1LP30) Vamp2* (P63045) Agfg1* (Q4KLH5) Arhgdia (Q5XI73) Sidt2* (D3ZEH5) Tuba1b (Q6P9V9) Arhgdia* (Q5XI73) Csrp1* (P47875) Stx7* (O70257) Dnajc5 (A0A0G2JX56) Nefm (P12839) Snx3* (Q5U211) Scamp1* (P56603) Baiap2* (Q6GMN2) Nudcd2 (Q5M823) Map1b (P15205)...”
- Chronic Cerebral Hypoperfusion-Induced Disturbed Proteostasis of Mitochondria and MAM Is Reflected in the CSF of Rats by Proteomic Analysis
Tukacs, Molecular neurobiology 2023 - “...S12 Protein synthesis ER, Cytoplasm P60901 Psma6 1.55 Proteasome subunit alpha type-6 Protein degradation Cytoplasm F1LP30 Mccc1 1.67 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Leucine catabolism Mitochondria P09606 Glul 1.31 Glutamine synthetase Glutamine metabolism Mitochondria, ER Cytoskeletal proteins Q6P9V9 Tuba1b 1.69 Tubulin alpha-1B chain Cytoskeletal protein Cytoskeleton...”
- Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats.
Fungfuang, Experimental animals 2023 - “...catabolic process to acetyl-CoA B5LSW7 Cysteine sulfinic acid decarboxylase Csad FVNVCFWFVPPSLRGKK Carboxylic acid metabolic process F1LP30 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial Mccc1, Mcca EHAGKIGYPVMIKAIR Leucine catabolic process F1LU71 Methylglutaconyl-CoA hydratase, mitochondrial Auh AYGKNSLSKNLLK Fatty acid beta-oxidation D4ACE9 Alpha-aminoadipic semialdehyde synthase, mitochondrial Aass APLAPKHIKGITK L-lysine catabolic process...”
- The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity.
Ji, PloS one 2022 - “...[ADP/GDP-forming] subunit alpha, mitochondrial 1.57 0.005037 D4A830_RAT D4A830 Ppa2 Pyrophosphatase (inorganic) 2 1.57 0.006482 F1LP30_RAT F1LP30 Mccc1 Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial 1.56 0.01137 D3ZTR1_RAT D3ZTR1 Mrps17 Mitochondrial ribosomal protein S17 1.56 0.004775 Q5RJR9_RAT Q5RJR9 Serpinh1 Serine (Or cysteine) proteinase inhibitor, clade H, member 1, isoform...”
PYC_MOUSE / Q05920 Pyruvate carboxylase, mitochondrial; Pyruvic carboxylase; PCB; EC 6.4.1.1 from Mus musculus (Mouse) (see paper)
46% identity, 38% coverage
- function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
catalytic activity: hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+) (RHEA:20844)
cofactor: biotin
cofactor: Mn(2+) (Binds 1 Mn(2+) ion per subunit.)
subunit: Homotetramer (By similarity). Interacts (via the biotin carboxylation domain) with SIRT4 (PubMed:23438705). - Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis
Banfi, Antioxidants (Basel, Switzerland) 2023 - “...Q63918 4 0.001 1.92 NEG Serum deprivation-response protein OS = Mus musculus GN = Sdpr Q05920 18 0.001 1.88 NEG Pyruvate carboxylase_ mitochondrial OS = Mus musculus GN = Pc P31786 2 0.021 1.87 NEG Acyl-CoA-binding protein OS = Mus musculus GN = Dbi O88492 3...”
- Proteomic profiling of the brain from the wobbler mouse model of amyotrophic lateral sclerosis reveals elevated levels of the astrogliosis marker glial fibrillary acidic protein
Murphy, European journal of translational myology 2023 - “...change Q99PU5 Long-chain-fatty-acid--CoA ligase ACSBG1 2 132.34 0.01572 4.14 Q9WUM4 Coronin-1C 3 144.85 0.00605 3.82 Q05920 Pyruvate carboxylase, mitochondrial 2 162.42 0.03267 3.61 Q00612 Glucose-6-phosphate 1-dehydrogenase X 2 79.96 0.01139 3.47 Q9WUB3 Glycogen phosphorylase 2 97.04 0.02155 2.55 Q8R0Y6 Cytosolic 10-formyltetrahydrofolate dehydrogenase 2 108.71 0.00524 2.44...”
- Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms
Soto, Nature 2023 - “...pull-down efficiency, all proteins and their LFQ values were normalized to pyruvate carboxylase (UniProt identifier Q05920 ). Downstream analysis was conducted only on proteins with non-zero LFQ values in three or more experimental replicates. Data analysis for whole bulk tissue analyses was carried out in an...”
- Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.
Zhi, International journal of biological sciences 2022 - “...Npm1 Nucleophosmin 9 31.8 32.56 0.65 1.62E-02 Q02819 Nucb1 Nucleobindin-1 12 32.9 53.408 1.51 2.06E-02 Q05920 Pc Pyruvate carboxylase 54 48.8 129.68 1.53 9.08E-05 P42669 Pura Transcriptional activator protein Pur-alpha 5 18.7 34.883 2.94 2.63E-02 Q80VJ2 Sra1 Steroid receptor RNA activator 1 2 9.1 25.557 3.50...”
- Proteomic profiling of the interface between the stomach wall and the pancreas in dystrophinopathy
Dowling, European journal of translational myology 2021 - “...P16675 Lysosomal protective protein Ctsa 3 0.017105 4.1 Q8K1B8 Fermitin 3 Fermt3 3 0.003082 3.6 Q05920 Pyruvate carboxylase, mitochondrial Pc 3 0.021666 3.4 Q99LP6 GrpE protein 1, mitochondrial Grpel1 3 0.00354 3.3 E9Q4Z2 Acetyl-CoA carboxylase 2 Acacb 3 0.022647 3.2 P51855 Glutathione synthetase Gss 3 0.008555...”
- Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models
Muraoka, Journal of proteome research 2021 - “...394.05 0.66 1.892 P24472 Gsta4 260.99 200.33 0.77 1.753 Q9CQN1 Trap1 131.26 98.76 0.75 1.512 Q05920 Pc 944.43 818.06 0.87 1.658 Q8BIP0 Dars2 27.08 18.13 0.67 1.895 Q6ZQK5 Acap2 811.17 944.92 1.16 1.452 P62242 Rps8 647.87 897.33 1.39 1.536 P62301 Rps13 600.46 848.51 1.41 1.364 Q8BT60...”
- Action potential-coupled Rho GTPase signaling drives presynaptic plasticity
O'Neil, eLife 2021 - “...of proteomics data Protein expression levels were intensity-scaled to the endogenously biotinylated proteins, pyruvate carboxylase (Q05920) and propionyl-CoA carboxylase (Q91ZA3). Imputation of missing values was performed after normalization ( Karpievitch et al., 2012 ) using the MinDet method ( Lazar et al., 2016 ). Missing values...”
- Adipocyte-Derived Extracellular Vesicles: State of the Art.
Rome, International journal of molecular sciences 2021 - “...adhesion molecule Mdh2 P08249 malate dehydrogenase 2, NAD (mitochondrial) Ogdh Q60597 oxoglutarate dehydrogenase (lipoamide) Pc Q05920 pyruvate carboxylase Pdhb Q9D051 pyruvate dehydrogenase (lipoamide) beta Pdia3 P27773 protein disulfide isomerase associated 3 Phb P67778 prohibitin Prkar2b P31324 protein kinase, cAMP dependent regulatory, type II beta Rab18 P35293...”
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NP_032823 pyruvate carboxylase, mitochondrial isoform 2 from Mus musculus
G5E8R3 Pyruvate carboxylase from Mus musculus
46% identity, 38% coverage
NP_001156418 pyruvate carboxylase, mitochondrial isoform 1 from Mus musculus
E9QPD7 Pyruvate carboxylase from Mus musculus
46% identity, 38% coverage
- Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.
Selen, The Journal of biological chemistry 2022 - GeneRIF: Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet.
- Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver.
Cappel, Cell metabolism 2019 - GeneRIF: Pyruvate-Carboxylase-Mediated Anaplerosis Promotes Antioxidant Capacity by Sustaining TCA Cycle and Redox Metabolism in Liver.
- The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells.
Li, Nature communications 2016 - GeneRIF: the MDM2-p53-PC signalling axis links mitochondrial metabolism to insulin secretion and glucose homeostasis, and could represent a therapeutic target in diabetes.
- Pyruvate carboxylase is critical for non-small-cell lung cancer proliferation.
Sellers, The Journal of clinical investigation 2015 - GeneRIF: These findings indicate that PC-mediated anaplerosis in early-stage NSCLC is required for tumor survival and proliferation.
- TR4 promotes fatty acid synthesis in 3T3-L1 adipocytes by activation of pyruvate carboxylase expression.
Park, FEBS letters 2014 (PubMed)- GeneRIF: TR4-mediated enhancement of fatty acid synthesis in adipocytes requires increased expression of PC gene
- Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
De, American journal of physiology. Endocrinology and metabolism 2012 (PubMed)- GeneRIF: Repression of pyruvate carboxylase could be interpreted as a survival tactic of repressing anabolic pathways to support a redirection of limited carbon units to catabolism rather than storage
- Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple gc boxes.
Rojvirat, Biochimica et biophysica acta 2011 (PubMed)- GeneRIF: Three GC boxes within the proximal promoter of the murine pyruvate carboxylase gene bind Sp1/Sp3
- Reduction of islet pyruvate carboxylase activity might be related to the development of type 2 diabetes mellitus in Agouti-K mice.
Han, The Journal of endocrinology 2010 - GeneRIF: Islet PC might play a role in the development of type 2 diabetes mellitus where reduction of PC activity might be a consequence of mild hyperglycemia and a cause for severe hyperglycemia.
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- Physiological and Biochemical Effects of Intrinsically High and Low Exercise Capacities Through Multiomics Approaches.
Tung, Frontiers in physiology 2019 - “...27.79 2.85 13.96 3.22 0.01815 6-Phosphofructokinase Q99K08 Q99K08 2.05 0.75 0 0 0.03472 Pyruvate carboxylase E9QPD7 E9QPD7 3.33 1.17 0 0 0.02909 Pyruvate carboxylase G5E8R3 G5E8R3 3.33 1.17 0 0 0.02909 Pyruvate carboxylase Q3T9S7 Q3T9S7 3.33 1.17 0 0 0.02909 Pyruvate carboxylase Q3TCQ3 Q3TCQ3 3.33 1.17...”
- Proteomics and bioinformatics analysis of mouse hypothalamic neurogenesis with or without EPHX2 gene deletion.
Zhong, International journal of clinical and experimental pathology 2015
BMEI0266 PYRUVATE CARBOXYLASE from Brucella melitensis 16M
46% identity, 38% coverage
- Comparative genomic analysis of Brucella melitensis vaccine strain M5 provides insights into virulence attenuation
Jiang, PloS one 2013 - “...[37] . For example, mutants of genes encoding sugar transport systems, such as pyruvate carboxylase (BMEI0266), ribokinase (BMEII0089), 6-phosphogluconate dehydrogenase (BMEII1124), inosamine methylase (BMEI1301), glycosyl transferase (BMEII1101), ribose transport (BMEII0300), and glucose/galactose transport (BMEII1053) limit carbon or energy supply and affect gene regulation. In addition, the...”
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics
He, Frontiers in cellular and infection microbiology 2012 - “...macrophages, HeLa 14979322 4 narG BMEII0950 Macrophages 12438693 5 norE BMEII1001 Mice 14979322 6 pyc BMEI0266 Macrophages 10678941 D: CELL CYCLE CONTROL, MITOSIS, AND MEIOSIS 7 virB5 BMEII0029 Macrophages 10510235 E: AMINO ACID TRANSPORT AND METABOLISM 8 aroC BMEI1506 Macrophages, HeLa, mice 11119550 9 aspC BMEI0516...”
A0QTE1 biotin carboxylase from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)
MSMEG_1807 acetyl-/propionyl-coenzyme A carboxylase alpha chain from Mycobacterium smegmatis str. MC2 155
MSMEG_1807, MSMEI_1762 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Mycolicibacterium smegmatis MC2 155
48% identity, 72% coverage
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “...3-Oxoadipate enol-lactonase (EC 3.1.1.24) 0.1 0.05 A0R617 pks13 , MSMEG_6392, MSMEI_6224 Polyketide synthase 0.16 0.14 A0QTE1 accA3 , MSMEG_1807, MSMEI_1762 Acetyl/propionyl-coenzyme A carboxylase alpha chain 0.13 0.17 A0QV28 glnB , MSMEG_2426, MSMEI_2365 Nitrogen regulatory protein P-II 0.06 0.08 0.24 0.16 Nucleic acid binding and metabolism, including...”
- Phosphoproteomic Approaches to Discover Novel Substrates of Mycobacterial Ser/Thr Protein Kinases.
Baros, Molecular & cellular proteomics : MCP 2020 (no snippet) - Mycobacterial DNA-binding protein 1 is critical for long term survival of Mycobacterium smegmatis and simultaneously coordinates cellular functions.
Enany, Scientific reports 2017 - “...binding 25 Catalase-peroxidase Up 7 A0A0C4N7T7 katG Catalase activity 26 Acetyl-/propionyl- coenzymeA carboxylase Up 5.8 A0QTE1 accA3 Biotin carboxylase activity 27 Alcohol dehydrogenase Up 5.5 A0QU52 MSMEG_2079 Oxido-reductase activity 29 Universal stress protein Up 3.3 A0QZA1 MSMEG_3950 Response to stress Enhancement of DNA synthesis on MDP1-defeciency...”
- An in vivo crosslinking system for identifying mycobacterial protein-protein interactions.
Lougheed, Journal of microbiological methods 2014 - “...(LpdC homologue) 5 A0R072 sucB (MSMEG_4283) 2-Oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase (DlaT homologue) 3 A0QTE1 MSMEG_1807 Acetyl-/propionyl-coenzyme A carboxylase alpha chain a 21 A0QS98 Tuf Elongation factor Tu 12 A0QQC8 dnaK Chaperone protein DnaK 9 A4ZHR8 MSMEG1842 Adenosylhomocysteinase 3 A0QTE7 MSMEG_1813 Propionyl-CoA carboxylase beta chain...”
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “...3.1.1.24) 0.1 0.05 A0R617 pks13 , MSMEG_6392, MSMEI_6224 Polyketide synthase 0.16 0.14 A0QTE1 accA3 , MSMEG_1807, MSMEI_1762 Acetyl/propionyl-coenzyme A carboxylase alpha chain 0.13 0.17 A0QV28 glnB , MSMEG_2426, MSMEI_2365 Nitrogen regulatory protein P-II 0.06 0.08 0.24 0.16 Nucleic acid binding and metabolism, including transcriptional regulators A0QTQ8...”
- Understanding Metabolic Remodeling in Mycobacterium smegmatis to Overcome Energy Exigency and Reductive Stress Under Energy-Compromised State
Patil, Frontiers in microbiology 2021 - “...FAS-I pathway such as fabD (MSMEG_4325), kasA (MSMEG_4327), kasB (MSMEG_4328), mabA (MSMEG_3150), accA3 (acetyl-CoA carboxylase; MSMEG_1807), accD4 (MSMEG_6391), accD6 (MSMEG_4329), and fas (MSMEG_4757) is observed in the atpD knock-out strain as compared to the wild-type and the complemented strain ( Figure 4A ). The observed downregulation...”
- “...pathway genes such as fabD (MSMEG_4325), kasA (MSMEG_4327), kasB (MSMEG_4328), mabA (MSMEG_3150), accA3 (acetyl-CoA carboxylase; MSMEG_1807), accD4 (MSMEG_6391), accD6 (MSMEG_4329), and fas (MSMEG_4757) in M. smegmatis wild-type (WT), atpD knock-out ( atpD ), and atpD -complemented ( atpD C ) strains. The transcript level in each...”
- Phosphoproteomic Approaches to Discover Novel Substrates of Mycobacterial Ser/Thr Protein Kinases
Baros, Molecular & cellular proteomics : MCP 2020 (secret) - Pupylated proteins are subject to broad proteasomal degradation specificity and differential depupylation
Laederach, PloS one 2019 - “...are pupylated to 50%, likely reflecting pupylation of one protomer in each dimer. The dimeric MSMEG_1807 also exhibits pupylation of one protomer, but shows a more complex band pattern, since about half of the protomers of the unpupylated starting material were truncated. The full-length, unpupylated monomer...”
- “...proteasomal degradation, the pupylated substrates show a broad range of depupylation rates. Pupylated FabD3KR and MSMEG_1807 are fully depupylated by Dop within the first two hours of the recorded time course. MSMEG_5049, PanB and MSMEG_2412 are processed within about three to five hours, and PckA as...”
- Transcriptional and post-translational regulation of AccD6 in Mycobacterium smegmatis
Liu, FEMS microbiology letters 2018 (PubMed)- “...we demonstrated that AccD6 of Mycobacterium smegmatis associates with AccA3 ( subunit of acetyl-CoA carboxylase, MSMEG_1807) and AccE ( subunit, MSMEG_1812) to form the acetyl-CoA (propionyl-CoA) carboxylase. Results showed that the MSMEG_4331 subunit is a regulator that interacts with the promoter region of accD6 to inhibit...”
- Mycobacteriophage Fruitloop gp52 inactivates Wag31 (DivIVA) to prevent heterotypic superinfection
Ko, Molecular microbiology 2018 - “...in which Wag31, or proteins known to interact with Wag31 complexes including CwsA (MSMEG_0023), AccA3 (MSMEG_1807), AccD4 (MSMEG_6391), and AccD5 (MSMEG_1813), were overexpressed and tested for their ability to rescue M. smegmatis from Fruitloop gp52 toxicity ( Fig. 2D ). Overexpression of Wag31 clearly results in...”
- “...mCherry from the P hsp60 promoter ( Oldfield & Hatfull, 2014 ). Wag31 , accA3 (MSMEG_1807 , coordinates 1883433 1885229 complement, with start codon changed from GTG to ATG), accD4 (MSMEG_6391 , coordinates 6455190 6456743 complement, with start codon changed from GTG to ATG), AccD5 MSMEG_1813...”
- Control of biotin biosynthesis in mycobacteria by a pyruvate carboxylase dependent metabolic signal
Lazar, Molecular microbiology 2017 - “...biotin-utilizing enzyme in trans , such as the acetyl/propionyl-CoA carboxylase alpha chain enzymes MSMEG_0334 or MSMEG_1807. Each of these proteins contains a BC and BCCP domain, but lacks a CT domain, which is present in the beta chain present on a separate polypeptide. In the E288K...”
- Monitoring global protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis under hypochlorite stress
Hillion, Scientific reports 2017 - “...16,0 6,5 15,0 20,1 MSMEG_1554 eutC Ethanolamine ammonia-lyase light chain 204* B 11,7 15,7 26,4 MSMEG_1807 accA3 Acetyl-/propionyl-CoA carboxylase alpha chain 236* B 13,4 12,6 26,0 22,8 25,7 45,7 MSMEG_1813 accD5 Methylmalonyl-CoA carboxyltransferase 356 (MSH;Cys) B 11,5 17,2 26,4 10,7 30,9 41,6 MSMEG_2207 MSMEG_2207 Beta-ketothiolase 9...”
- More
- Interactome Analysis Identifies MSMEI_3879 as a Substrate of Mycolicibacterium smegmatis ClpC1
Ogbonna, Microbiology spectrum 2023 - “...0.1 0.05 A0R617 pks13 , MSMEG_6392, MSMEI_6224 Polyketide synthase 0.16 0.14 A0QTE1 accA3 , MSMEG_1807, MSMEI_1762 Acetyl/propionyl-coenzyme A carboxylase alpha chain 0.13 0.17 A0QV28 glnB , MSMEG_2426, MSMEI_2365 Nitrogen regulatory protein P-II 0.06 0.08 0.24 0.16 Nucleic acid binding and metabolism, including transcriptional regulators A0QTQ8 rhlE...”
BLJAPNOD_00700 pyruvate carboxylase from Ensifer sp. M14
46% identity, 38% coverage
DU507_11025, LGG_02112 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Lacticaseibacillus rhamnosus GG
LGG_02112 acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha / biotin carboxylase from Lactobacillus rhamnosus GG
50% identity, 98% coverage
Q7YS28 Pyruvate carboxylase from Sus scrofa
46% identity, 38% coverage
- Label-Free Quantitative Analysis of Pig Liver Proteome after Hepatitis E Virus Infection
Martino, Viruses 2024 - “...mitochondrial 24 147.20 0.0039 1.98 P42174 GLUD1 glutamate dehydrogenase 1, mitochondrial 23 133.50 0.0007 2.01 Q7YS28 PC pyruvate carboxylase, mitochondrial 23 113.27 0.0033 10.54 A0A4X1URZ5 ACSS3 acyl-CoA synthetase medium-chain family member 4 22 121.18 0.0017 2.01 A0A480SA59 SARDH sarcosine dehydrogenase, mitochondrial isoform X1 22 107.97 0.0004...”
- Insights into the Protein Differentiation Mechanism between Jinhua Fatty Ham and Lean Ham through Label-Free Proteomics.
Huang, Foods (Basel, Switzerland) 2023 - “...5.09 0.03 4.78 0.09 2.02 0.00 A0A287AY74 FBP2 Fructose-bisphosphatase 7.53 0.02 7.22 0.09 2.02 0.00 Q7YS28 Pyruvate carboxylase 5.56 0.10 5.24 0.18 2.01 0.05 A0A2K6CXN1 NEB SH3 domain-containing protein 5.85 0.10 5.54 0.14 1.96 0.05 A0A5G2Q828 PNPO Pyridoxal 5-phosphate synthase 5.79 0.02 5.48 0.16 1.96 0.01...”
- An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients
Poulsen, Frontiers in molecular neuroscience 2017 - “...697 127 I3LJH3 Pyrroline-5-carboxylate reductase 1, mitochondrial * 13.291 1,617 1,324 3,021 544 4,293 428 Q7YS28 Pyruvate carboxylase 129.612 1,098 1,345 11,838 3,447 6,245 3,488 2,889 1,488 1,596 1,158 12,681 2,910 9,132 800 3,343 1,677 P08835 Serum albumin 69.692 3,151 1,542 2,413 1,251 2,189 804 3,930...”
P52873 Pyruvate carboxylase, mitochondrial from Rattus norvegicus
46% identity, 38% coverage
- Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome
Barker, The Biochemical journal 2024 - “...(P22734) Hn1(Q6AXU6) Hsph1 (Q66HA8) Map1a (P34926) C2cd2l (Q5U2P5) Hn1* (Q6AXU6) Asap1* (Q1AAU6) Hsp90b1 (Q66HD0) Pc (P52873) Stxbp1* (P61765) Cdv3* (A0A0G2K0B0) Vamp2* (P63045) Stip1 (O35814) Prph (P21807) Pcdh1* (A0A0G2K6T9) Dpysl2* (P47942) Sept11 (A0A0G2JUL7) Pcca (A0A0G2K401) Nudcd2* (Q5M823) Txn1* (P11232) Sept8 (G3V9Z6) Hsp90aa1 (P82995) Myh11 (E9PTU4) Sprr1a* (G3V755)...”
- Proteomics to Metabolomics: A New Insight into the Pathogenesis of Hypertensive Nephropathy
Eshraghi, Kidney & blood pressure research 2023 - “...3.476 0.004 G3V734 Decr1 2,4-dienoyl-Coa reductase 1 3.212 0.033 D3Z8F1 Vill Villin like 3.185 0.021 P52873 Pc Pyruvate carboxylase 3.052 0.038 G3V7L4 Cdh16 Cadherin 16 3.049 0.023 F1LQC1 Acox1 Acyl-coenzyme A oxidase 3.041 0.018 P08461 Dlat Dihydrolipoyllysine-residue acetyltransferase 2.998 0.039 PXD012889 (outer cortex) P08932 Kng1 Kininogen...”
- “...lipoprotein receptor 4.32 0.009 Q68FT3 Pyroxd2 Pyridine nucleotide-disulphide 4.066 0.006 P08461 Dlat Dihydrolipoamide-S-acetyltransferase 4.054 0.016 P52873 Pc Pyruvate carboxylase 3.963 0.047 G3V7L4 Cdh16 Cadherin 16 3.528 0.044 G3V6S5 Mthfd1 Methylenetetrahydrofolate dehydrogenase 3.434 0.007 G3V734 Decr1 2,4-dienoyl-Coa reductase 1 2.972 0.042 P97608 Oplah 5-oxoprolinase, ATP-hydolyzing 2.907 0.042...”
- Diet-Induced Hypercholesterolemia Leads to Cardiac Dysfunction and Alterations in the Myocardial Proteome
Szabó, International journal of molecular sciences 2022 - “...Mug1 Murinoglobulin-1 1.96 O35814 Stip1 Stress-induced-phosphoprotein 1 1.91 P09006 Serpina3n Serine protease inhibitor A3N 1.90 P52873 Pc Pyruvate carboxylase 1.54 P02680 Fgg Fibrinogen gamma chain 1.45 P02564 Myh7 Myosin-7 1.42 P06399 Fga Fibrinogen alpha chain 1.42 P01026 C3 Complement C3 1.35 D3ZWC6 Sntb1 Syntrophin, basic 1...”
- Extracellular vesicles released by steatotic hepatocytes alter adipocyte metabolism
Mleczko, Journal of extracellular biology 2022 - “...lyase, mitochondrial SDC4_RAT P34901 1.78 1.17E04 Syndecan4 CP2DQ_RAT P10634 1.79 1.03E03 Cytochrome P450 2D26 PYC_RAT P52873 1.82 1.83E03 Pyruvate carboxylase, mitochondrial RS27A_RAT P62982 1.84 8.94E04 Ubiquitin40S ribosomal protein S27a RDH2_RAT P50170 1.86 4.16E04 Retinol dehydrogenase 2 ACOX2_RAT P97562 1.87 3.22E02 Peroxisomal acylcoenzyme A oxidase 2 HYEP_RAT...”
- Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.
Camino, International journal of molecular sciences 2022 - “...Medium-chain specific acyl-CoA dehydrogenase, mitochondrial 0.047 2.13 THIL_RAT P17764 Acetyl-CoA acetyltransferase, mitochondrial 0.006 2.12 PYC_RAT P52873 Pyruvate carboxylase, mitochondrial 0.003 2.10 IDH3A_RAT Q99NA5 Isocitrate dehydrogenase [NAD] subunit Alpha, mitochondrial 0.027 2.07 HSP74_RAT O88600 Heat shock 70 kDa protein 4 0.044 2.04 FETA_RAT P02773 Alpha-fetoprotein 0.029 2.01...”
- Red-Fleshed Apples Rich in Anthocyanins and White-Fleshed Apples Modulate the Aorta and Heart Proteome in Hypercholesterolaemic Rats: The AppleCOR Study.
Catalán, Nutrients 2022 - “...1.670 0.0022 P43278 H1f0 Histone H1.0 1.259 0.0110 P11980 Pkm Pyruvate kinase PKM 1.268 0.0128 P52873 Pc Pyruvate carboxylase, mitochondrial 1.538 0.0140 Q9EQS0 Taldo1 Transaldolase 1.293 0.0187 M0RBF1 C3 Complement C3 2.158 <0.0001 G3V7K3 Cp Ceruloplasmin 2.244 <0.0001 G3V7N9 C1qb Complement C1q subcomponent subunit B 2.554...”
- Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats
Leão, Oxidative medicine and cellular longevity 2021 - “...and P21571), Citrate Synthase (Q8VHF5), Cytochrome C Oxidase subunits (P00406, P11240, and P10818), Pyruvate carboxylase (P52873), and Pyruvate dehydrogenase (P26284) that are involved in glucose metabolism and affect the cell energy balance, suggesting an impairment on cell energy metabolism. The failure on energy metabolism may impact...”
- Inflammation and apoptosis accelerate progression to irreversible atrophy in denervated intrinsic muscles of the hand compared with biceps: proteomic analysis of a rat model of obstetric brachial plexus palsy.
Yu, Neural regeneration research 2020 - “...F1LLZ7, A2RRU1, P97531, G3V8V3, Q4G050, A0A0G2K9C8, Q6P727, Q9Z1N1 0.002 Pyruvate metabolism 17 A0A0G2K5G8 * , P52873 * , Q6P7Q4, P11980, P04636, A0A0G2K4C6, P35745, F7FKI5, P17764, O88989, P42123, P08461, P14408, Q6P6R2, A0A0G2JZH8, P49432, B0BN46 < 0.001 HIF-1 pathway 14 Q3HSE5 * , Q9ET45 * , P15429, M0RD75,...”
- More
XP_006230753 pyruvate carboxylase, mitochondrial isoform X2 from Rattus norvegicus
46% identity, 38% coverage
- Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.
Wutthisathapornchai, PloS one 2014 - GeneRIF: Data indicate that glucose-mediated transcriptional activation of the pyruvate carboxylase (PC) gene is regulated by transcription factors.
- Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.
MacDonald, Archives of biochemistry and biophysics 2013 - GeneRIF: Pyruvate carboxylase and fatty acid synthase were knocked down in insulin cells. Insulin secretion, phospholipid and cholesterol esters were lowered.
- Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter.
Pedersen, The Biochemical journal 2010 (PubMed)- GeneRIF: A carbohydrate response element in the distal gene promoter contributes to the glucose-mediated expression of pyruvate carboxylase.
- Expression of pyruvate carboxylase in cultured oligodendroglial, microglial and ependymal cells.
Murin, Neurochemical research 2009 (PubMed)- GeneRIF: PC is not only expressed in cultured astroglial cells but also in cultured oligodendrocytes, microglial cells, and ependymocytes.
- Impaired anaplerosis and insulin secretion in insulinoma cells caused by small interfering RNA-mediated suppression of pyruvate carboxylase.
Hasan, The Journal of biological chemistry 2008 - GeneRIF: PC is important for insulin secretion stimulated by noncarbohydrate insulin secretagogues
- The role of pyruvate carboxylase in insulin secretion and proliferation in rat pancreatic beta cells.
Xu, Diabetologia 2008 - GeneRIF: The PC pathway in beta cells may play a key role in pyruvate metabolism, insulin secretion and cell proliferation.
- Transcriptional regulation of the distal promoter of the rat pyruvate carboxylase gene by hepatocyte nuclear factor 3beta/Foxa2 and upstream stimulatory factors in insulinoma cells.
Boonsaen, The Biochemical journal 2007 - GeneRIF: The results presented here show that Foxa2 and USFs regulate the distal promoter of the rat PC gene in a cell-specific manner.
- Compensatory responses to pyruvate carboxylase suppression in islet beta-cells. Preservation of glucose-stimulated insulin secretion.
Jensen, The Journal of biological chemistry 2006 (PubMed)- GeneRIF: beta-cells activate compensatory mechanisms in response to suppression of PC expression that prevent impairment of anaplerosis, pyruvate cycling, NAPDH production, and GSIS
- More
8sgxX / A0A640KC69 Leishmania tarentolae propionyl-coa carboxylase (alpha-4-beta-6) (see paper)
46% identity, 67% coverage
- Ligand: 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal (8sgxX)
Q29RK2 pyruvate carboxylase (EC 6.4.1.1) from Bos taurus (see paper)
46% identity, 38% coverage
Cj1037c putative pyruvate carboxylase A subunit from Campylobacter jejuni subsp. jejuni NCTC 11168
45% identity, 93% coverage
- The Novel Protein Cj0371 Inhibits Chemotaxis of Campylobacter jejuni
Du, Frontiers in microbiology 2018 - “...Aconitate hydratase activity 92793 1 cj1437c Sugar transferase 41929 1 cj1487c Oxidative phosphorylation 31162 1 cj1037c ATP binding pyruvate carboxylase activity 54413 2 cj0933c Ribose-phosphate pyrophosphokinase 65834 8 Bacterial chemotaxis cj0285c ( cheV ) Chemotaxis protein 35858 9 cj0284c ( cheA ) Chemotaxis protein 85300 3...”
- Defining the metabolic requirements for the growth and colonization capacity of Campylobacter jejuni
Hofreuter, Frontiers in cellular and infection microbiology 2014 - “...the PEP carboxykinase PckA (Cj0932c), the pyruvate kinase Pyk (Cj0392c) and the pyruvate carboxylase (PycA, Cj1037c; PycB, Cj0933c) comprise a crucial metabolic junction between catabolism and anabolism in C. jejuni (Velayudhan and Kelly, 2002 ). These enzymes are required for the oxaloacetatePEPpyruvate conversion that comprises a...”
Q3T9S7 Pyruvate carboxylase from Mus musculus
46% identity, 38% coverage
Atu2726 pyruvate carboxylase from Agrobacterium tumefaciens str. C58 (Cereon)
48% identity, 36% coverage
Tfu_2557 putative acyl-CoA carboxylase, alpha subunit from Thermobifida fusca YX
WP_011292980 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Thermobifida fusca YX
47% identity, 72% coverage
- The Effects of Carbon Source and Growth Temperature on the Fatty Acid Profiles of Thermobifida fusca
Winkelman, Frontiers in molecular biosciences 2022 - “...subunits Tfu_0947 ACCase B ACCase CT subunit Tfu_0948 AcCCase A AcCCase BC and BCCP subunit Tfu_2557 AcCCase B AcCCase CT subunit Tfu_2555 AcCCase E AcCCase E subunit Tfu_2556 ACCase B Additional ACCase CT subunit Tfu_1228, Tfu_1215 BCCP Biotin Carboxyl-Carrier Protein Tfu_1513 LCCase LCCase Tfu_1530 AcpP Acyl-carrier...”
- “...the T. fusca genome. Previous studies have experimentally characterized the T. fusca operon (Tfu_2555, Tfu_2556, Tfu_2557) that encodes the B, E, and A subunits of an acyl-CoA carboxylase (AcCCase) ( Shivaiah et al., 2021 ). This enzyme is promiscuous and can carboxylate acetyl-CoA, propionyl-CoA, and butyryl-CoA....”
- Kinetic, Structural, and Mutational Analysis of Acyl-CoA Carboxylase From Thermobifida fusca YX
Shivaiah, Frontiers in molecular biosciences 2020 - “...2017 ). Methods and Materials Recombinant Expression Vectors ORFs encoding the TfAcCCB (Tfu_2555; WP_011292978), TfAcCCA (Tfu_2557; WP_011292980), and TfAcCCE (Tfu_2556; WP_011292979) proteins were initially identified using The Thioester-active enzYme database (ThYme) ( http://www.enzyme.cbirc.iastate.edu/ ) (Cantu et al., 2010 , 2011 ; Chen et al., 2011 )....”
- “...). Based on these sequence similarities, the three T. fusca ORFs where characterized as: (a) Tfu_2557 (WP_011292980) encoding the TfAcCCA subunit (62.1 kDa) that appears to carry the BC and BCCP functional components; (b) Tfu_2555 (WP_011292978) encoding the TfAcCCB subunit (57.6 kDa) that carries the CT...”
- Proteomics-based metabolic modeling and characterization of the cellulolytic bacterium Thermobifida fusca
Vanee, BMC systems biology 2014 - “...autobuilt Tfu_v2 version whereas the proteomics version clearly shows its presence (EC 6.4.1.1, Gene ID: Tfu_2557, Tfu_1530, Tfu_0947, Tfu_1228). In addition, most of the amino acid pathways were fully or partially incomplete in Tfu_v2. Thus, the Tfu_v2 model had artificially high fluxes through transport reactions to...”
- Kinetic, Structural, and Mutational Analysis of Acyl-CoA Carboxylase From Thermobifida fusca YX
Shivaiah, Frontiers in molecular biosciences 2020 - “...). Methods and Materials Recombinant Expression Vectors ORFs encoding the TfAcCCB (Tfu_2555; WP_011292978), TfAcCCA (Tfu_2557; WP_011292980), and TfAcCCE (Tfu_2556; WP_011292979) proteins were initially identified using The Thioester-active enzYme database (ThYme) ( http://www.enzyme.cbirc.iastate.edu/ ) (Cantu et al., 2010 , 2011 ; Chen et al., 2011 ). The...”
- “...Based on these sequence similarities, the three T. fusca ORFs where characterized as: (a) Tfu_2557 (WP_011292980) encoding the TfAcCCA subunit (62.1 kDa) that appears to carry the BC and BCCP functional components; (b) Tfu_2555 (WP_011292978) encoding the TfAcCCB subunit (57.6 kDa) that carries the CT functional...”
PCCA_RUEPO / Q5LUF3 Propionyl-CoA carboxylase alpha chain; EC 6.4.1.3 from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see paper)
Q5LUF3 propionyl-CoA carboxylase (EC 6.4.1.3) from Ruegeria pomeroyi (see paper)
45% identity, 65% coverage
- function: This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), the enzyme catalyzing the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl- CoA/(S)-methylmalonyl-CoA (PubMed:20725044). Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then tranfers the carboxyl group from carboxylated biotin to propionyl-CoA (Probable).
catalytic activity: propanoyl-CoA + hydrogencarbonate + ATP = (S)-methylmalonyl- CoA + ADP + phosphate + H(+) (RHEA:23720)
cofactor: Mg(2+) Mn(2+) (Binds 2 magnesium or manganese ions per subunit.)
cofactor: biotin
subunit: The holoenzyme is a dodecamer composed of 6 PccA/alpha subunits and 6 PccB/beta subunits. - 3-methylcrotonyl-CoA carboxylase deficiency: clinical, biochemical, enzymatic and molecular studies in 88 individuals
Grünert, Orphanet journal of rare diseases 2012 - “...as well as the structurally characterized P. aeruginosa MCCC1 (paMCCC1, Q9I299), Ruegeria pomeroyi PCC (bPCCA, Q5LUF3), human ACC1 (hACC1, Q13085) and ACC2 (hACC2, O00763). Novel MCCC1 missense mutations are asterisked. The electrostatic interaction between Glu288 and Arg444 in MCCC1 is highlighted in green. Click here for...”
XP_005227044 pyruvate carboxylase, mitochondrial isoform X1 from Bos taurus
46% identity, 37% coverage
G7YW97 Propionyl-CoA carboxylase alpha chain from Clonorchis sinensis
47% identity, 58% coverage
LSEI_2111 Biotin carboxylase from Lactobacillus casei ATCC 334
50% identity, 99% coverage
PP4067, PP_4067 acetyl-CoA carboxylase, biotin carboxylase, putative from Pseudomonas putida KT2440
49% identity, 67% coverage
WD0433 propionyl-CoA carboxylase, alpha subunit from Wolbachia endosymbiont of Drosophila melanogaster
43% identity, 64% coverage
SRX10_001739 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha from Lacticaseibacillus paracasei
50% identity, 99% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory