PaperBLAST
PaperBLAST Hits for tr|Q9I4D1|Q9I4D1_PSEAE Glutathione-regulated potassium-efflux system protein KefB OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=kefB PE=3 SV=1 (613 a.a., MEHGSSFLQS...)
Show query sequence
>tr|Q9I4D1|Q9I4D1_PSEAE Glutathione-regulated potassium-efflux system protein KefB OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=kefB PE=3 SV=1
MEHGSSFLQSGVVFLIAAVFMVPLAKRLQLGAVLGYLLAGVLIGPSVLGLVDNPESVAQI
SEMGVVLLLFIIGLELSPRRLWVMRKSVFGVGLLQVLLTGLVIGTVALVGFDQPVNSAVV
LGLGLALSSTAFGLQILAERKELTQPHGRLAFAILLFQDIAAIPLIAMIPLLSGAHSDAG
GSELTQTLKVVGSIAAVVVGGRYLLRPVFRIVAQTKLQDMSTATALLVVMGTALLMELAG
VSMALGAFLAGLLLADSEYRHELEAQIEPFKGLLLGLFFISVGMSADISLLLKSPWMVLG
LTVLLIALKAPVLYFVGRISGGLDKASALRLGVVLAAGGEFAFVVFKMARDQGLFQNDLH
NLLVLSITLSMALTPLLVLGLARLLAKEPEATKPPPEMERIDNDDTPRVVIAGVGRMGQI
VARVLRAQRVPFIALDTSVETIELTRTLGAIPIFYGDPMRPEILRAAQVEKAEFFVIATD
DPETNIETAKRVRKLYPHLKIIARARNRQHVHLLLDAGVEPVRETFFSSLEMSRMVLCGL
GLSEEQADARIRRFRRHDEEVLASQHLFYNDRDALIKSAREARVELETLFQTDQLEDRGA
VAAETPEKPSRAS
Running BLASTp...
Found 237 similar proteins in the literature:
PA1207 glutathione-regulated potassium-efflux system protein KefB from Pseudomonas aeruginosa PAO1
NP_249898 glutathione-regulated potassium-efflux system protein KefB from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
- An innovative approach to decoding genetic variability in Pseudomonas aeruginosa via amino acid repeats and gene structure profiles
Kim, Scientific reports 2024 - “...2022 6 19 773 GCA_033217895.1 10 ATCC27853 Netherlands 2014 6 15 A 2 GCA_001618925.1 11 Pa1207 Mexico, Mexico city 2012 6 13 A 2 GCF_002208645.1 12 NCGM257 Japan, Tokyo 2004 8 11 B 1 357 GCA_001547955.1 13 USDA-ARS-USMARC-41,639 USA, Kansas 2013 8 11 B 5 GCA_001518975.1...”
- “...China, Hangzhou 2022 GCA_030034675.1 6 WTJH17 N.D. 2018 GCA_018138065.1 7 FDAARGOS_532 N.D. 2016 GCA_003812165.1 8 Pa1207 Mexico, Mexico city 2012 GCF_002208645.1 9 ATCC27853 Netherlands 2014 GCA_001618925.1 10 SE5419 China, Jiangsu 2013 GCF_019720855.1 11 DHS01 France 1997 GCA_000496455.2 12 PAO1 N.D. 2014 GCA_000006765.1 13 PALA24 United Kingdom,...”
- Microbiological and molecular studies on a multidrug-resistant Pseudomonas aeruginosa from a liver transplant patient with urinary tract infection in Egypt
Shaaban, BMC microbiology 2024 - “...2 Pa127 CP022000 Mexico 4138 2307 78 0 Pa1242 CP022002 Mexico 4138 2261 193 4 Pa1207 CP022001 Mexico 4138 2454 196 7 97 CP031449 Ghana 4138 2413 42 0 CCUG 70744 CP023255 Sweden 4138 2296 14 7 Y89 CP030913 South Korea 4138 2179 6 1 Y82...”
- Persistent Bacterial Coinfection of a COVID-19 Patient Caused by a Genetically Adapted Pseudomonas aeruginosa Chronic Colonizer
Qu, Frontiers in cellular and infection microbiology 2021 - “...selected species including PAO1 reference strain and virulent clinical strains, PA14, PA 34, DK2, and Pa1207 isolated from patients with different diseases like cystic fibrosis, keratitis, and bacteremia to predict specific genomic islands (GIs) on LYSZa7 genome ( Figure 4 ). As seen from the circular...”
- “...Circle 7: P. aeruginosa DK2 (Strain isolated from Cystic Fibrosis patient); Circle 8: P. aeruginosa Pa1207 (strain isolated from Bacteremia patient); Circle 9: Genomic Islands predicted with specific GIs of LYSZa7 highlighted in red; Circle 10: Antimicrobial resistance genes (ARGs). DNA methylation as part of DNA...”
- Beyond Homeostasis: Potassium and Pathogenesis during Bacterial Infections
Do, Infection and immunity 2021 (secret) - Persistent bacterial coinfection of a COVID-19 patient caused by a genetically adaptedPseudomonas aeruginosachronic colonizer
Qu, 2020 - Seawater salt-trapped Pseudomonas aeruginosa survives for years and gets primed for salinity tolerance
Elabed, BMC microbiology 2019 - “...slightly induced in the T48 variant, including, the glutathione-regulated K + -efflux system protein KefB (PA1207; 1.5-fold regulation) and a putative K + channel (PA1496; 1.52-fold regulation). K + transporters are regulated by an increase in environment osmolarity regardless of the solute used and turgor. This...”
- Complete Genome Sequences of Two Pseudomonas aeruginosa Strains Isolated from Children with Bacteremia
Espinosa-Camacho, Genome announcements 2017 - “...hospitalization in the intensive care unit (ICU), and prematurity ( 1 ). Two P.aeruginosa strains (Pa1207 and Pa1242) were sequenced. The strains were isolated from children with bacteremia admitted to a pediatric hospital in Mexico City. Strain Pa1207 was resistant to different -lactams (including carbapenems and...”
- “...v3 ( 2 ). The final assemblies had mean coverages of 146 and 181 for Pa1207 and Pa1242, respectively, and consisted of chromosomes of 7,411,863bp and 7,050,510bp, with mean G+C contents of 65.7% and 65.8%, respectively. A total of 7,153 genes were annotated for Pa1207: 7,072...”
- Tyrosine Phosphatase TpbA of Pseudomonas aeruginosa Controls Extracellular DNA via Cyclic Diguanylic Acid Concentrations
Ueda, Environmental microbiology 2010 - “...five loci to amplify DNA fragments by PCR. Using the primers for tpbB (PA1120), kchA (PA1207), knaA (PA5021), rhlI (PA3476), and pvdS (PA2426), DNA fragments were successfully amplified from both eDNA samples from the supernatant of bacterial cultures and from gDNA purified from bacterial cells (...”
- More
- Potassium and sodium transporters of Pseudomonas aeruginosa regulate virulence to barley.
Ueda, Applied microbiology and biotechnology 2008 (PubMed)- GeneRIF: PAO1 displayed reduced barley virulence with inactivated PA1207, PA5021, and PA2647.
PP_5246 glutathione-regulated potassium-proton antiporter from Pseudomonas putida KT2440
68% identity, 97% coverage
Achr_20870 monovalent cation:proton antiporter-2 (CPA2) family protein from Azotobacter chroococcum NCIMB 8003
62% identity, 97% coverage
C5975_00475, ESA_04389 glutathione-regulated potassium-efflux system protein KefB from Cronobacter sakazakii ATCC BAA-894
46% identity, 97% coverage
- Effect of glutathione-transport-related gene gsiD on desiccation tolerance of Cronobacter sakazakii and its related regulatory mechanism
Wang, Applied and environmental microbiology 2024 (secret) - Characterization of the Desiccation Tolerance of Cronobacter sakazakii Strains
Du, Frontiers in microbiology 2018 - “...GGGCAAATGCGTAGTGGAGT R: GCTTCGTCGGTGAGATCGT kdpA (ESA_02641) Potassium transporting ATPase subunit A F: CGTGGGGGCTTGGCTTTAT R: CGCCGCAGTTTCCAGAGTT kefB (ESA_04389) Glutathione-regulated potassium-efflux system protein KefB F: TTACGACCAGGGCTACACACG R: CGGACTACGACGCACTTTATGAG betA (ESA_02049) Choline dehydrogenase F: GCCCTACTACCGTAAGTCGGAAAC R: CCTGCACGCCTGCTTCTATCAT betB (ESA_02048) Betaine aldehyde dehydrogenase F: CGCCACGGGTAACACTTT R: CCGCTTCCACGGCACTA 16S rRNA 16S rRNA...”
- “...in the strain with the second strongest desiccation tolerance (ENS 71106), only three genes (ESA_03328, ESA_04389, and ESA_02049) showed significantly higher expression levels in comparison with that of SAKA 90814. The remaining genes (ESA_02131, ESA_01738, ESA_01740, ESA_02641, ESA_01334, ESA_01335, and ESA_02048) showed similar expression levels in...”
- Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains
Jang, Standards in genomic sciences 2018 - “...threonine, homoserine lactone (locus tag C5975_00275), p-hydroxybenzoic acid (locus tag C5975_07280), glutathione-regulated potassium (locus tag C5975_00475, C5975_00480, C5975_08855, C5975_08860, KefGFCB), RND efflux (C5975_02520, Transporter), proteins associated with heavy metal efflux of nickel/cobalt (C5975_13445, RcnB), cobalt/magnesium (C5975_08880, ApaG), and manganese ions (C5975_18840, MntP), sugar efflux (C5975_13720, SetB),...”
A1S_0367 glutathione-regulated potassium-efflux system protein (K(+)/H(+) antiporter) from Acinetobacter baumannii ATCC 17978
44% identity, 90% coverage
SEN_RS17095 glutathione-regulated potassium-efflux system protein KefB from Salmonella enterica subsp. enterica serovar Enteritidis str.
45% identity, 97% coverage
TrkB / b3350 K+ : H+ antiporter KefB from Escherichia coli K-12 substr. MG1655 (see 7 papers)
kefB / P45522 K+ : H+ antiporter KefB from Escherichia coli (strain K12) (see 7 papers)
KEFB_ECOLI / P45522 Glutathione-regulated potassium-efflux system protein KefB; K(+)/H(+) antiporter; NEM-activable K(+)/H(+) antiporter from Escherichia coli (strain K12) (see 3 papers)
TC 2.A.37.1.2 / P45522 Glutathione-regulated potassium-efflux system protein kefB, component of Glutathione-regulated K+ efflux protein B, KefB; regulated by ancillary protein KefG (YheR) from Escherichia coli (strain K12) (see 4 papers)
45% identity, 97% coverage
- function: Pore-forming subunit of a potassium efflux system that confers protection against electrophiles. Catalyzes K(+)/H(+) antiport.
subunit: Interacts with the regulatory subunit KefG. - substrates: K+
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments.
Armbruster, Nature communications 2014 - “...) 37 . Sequence comparison of TM5 of the CPA2 domain of AtKEA3, EcNhaA, EcKefB (P45522) EcKefC, TtNapA, Bacillus cereus GerN (BcGerN, Q9KI10), Synechocystis NhaS3 (Q55190) and NhaS4 (Q5N3F5), Arabidopsis AtCHX17 (Q9SUQ7), AtKEA2 and Saccharomyces cerevisiae KHA1 (P40309) were performed using ClustalOmega. KEA3 co-expression analysis was...”
- Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant
Wei, Microbiology (Reading, England) 2007 - “...L2TR Q60362 BAC61130 ABC30246.1 AAV82810.1 NP_414589 P45522 Escherichia coli K12 Escherichia coli K12 ZP_01126363.1 YP_114199.1| Nitrococcus mobilis Nb-231...”
- Mutational loss of a K+ and NH4+ transporter affects the growth and endospore formation of alkaliphilic Bacillus pseudofirmus OF4
Wei, Journal of bacteriology 2003 - “...E. coli B. subtilis Staphylococcus aureus F69819 C70474 P45522 S39662 F89941 34/44 (84) E. coli Q47537 59/75 (313) 65/82 (570) 34/55 (118) Bacillus halodurans...”
HBB05_RS02355 glutathione-regulated potassium-efflux system protein KefB from Pantoea agglomerans
44% identity, 97% coverage
- Comprehensive genomic analysis reveals virulence factors and antibiotic resistance genes in Pantoea agglomerans KM1, a potential opportunistic pathogen
Guevarra, PloS one 2021 - “...ProQ HBB05_RS14815 otsA , otsB Trehalose-6-phosphate synthase HBB05_RS15190, HBB05_RS15195 kefB , Glutathione-regulated potassium-efflux system protein HBB05_RS02355, HBB05_RS02360 TreF Cytoplasmic trehalase HBB05_RS00975 Heat shock DnaJ , DnaK Molecular chaperone DnaJ HBB05_RS18860, HBB05_RS19155, HBB05_RS07025, HBB05_RS07020 hslR Ribosome-associated heat shock protein Hsp15 HBB05_RS02565 IbpA , hspQ Heat shock protein...”
LIMLP_17560 monovalent cation:proton antiporter-2 (CPA2) family protein from Leptospira interrogans serovar Manilae
42% identity, 97% coverage
TrkC / b0047 K+ : H+ antiporter KefC from Escherichia coli K-12 substr. MG1655 (see 11 papers)
kefC / P03819 K+ : H+ antiporter KefC from Escherichia coli (strain K12) (see 11 papers)
KEFC_ECOLI / P03819 Glutathione-regulated potassium-efflux system protein KefC; K(+)/H(+) antiporter from Escherichia coli (strain K12) (see 6 papers)
TC 2.A.37.1.1 / P03819 KefC aka TRKC aka B0047, component of Glutathione-regulated K+ efflux protein C, KefC; regulated by ancillary protein KefF (YabF) from Escherichia coli (see 7 papers)
NP_414589 K(+) : H(+) antiporter KefC from Escherichia coli str. K-12 substr. MG1655
b0047 glutathione-regulated potassium-efflux system protein from Escherichia coli str. K-12 substr. MG1655
43% identity, 98% coverage
- function: Pore-forming subunit of a potassium efflux system that confers protection against electrophiles. Catalyzes K(+)/H(+) antiport. Can also export rubidium, lithium and sodium.
subunit: Homodimer. Interacts with the regulatory subunit KefF, forming a heterotetramer with 2:2 stoichiometry. Interaction with KefF is required for optimal activity. The active antiporter may be formed by the heterotetramer, or by an octamer. - substrates: K+
- Structure and mechanism of the K+/H+ exchanger KefC.
Gulati, Nature communications 2024 - GeneRIF: Structure and mechanism of the K[+]/H[+] exchanger KefC.
- Two members of a network of putative Na+/H+ antiporters are involved in salt and pH tolerance of the freshwater cyanobacterium Synechococcus elongatus
Billini, Journal of bacteriology 2008 - “...dendrogram: KefC (E. coli) (accession number NP_414589), NhaP (Pseudomonas aeruginosa PAO1) (accession number NP_252576), NHX1 (Saccharomyces cerevisiae)...”
- Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant
Wei, Microbiology (Reading, England) 2007 - “...loihiensis L2TR Q60362 BAC61130 ABC30246.1 AAV82810.1 NP_414589 P45522 Escherichia coli K12 Escherichia coli K12 ZP_01126363.1 YP_114199.1| Nitrococcus mobilis...”
- Regulation and Levels of the Thylakoid K+/H+ Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light
Armbruster, Plant & cell physiology 2016 - “...web.expasy.org/peptide_cutter ). For sequence comparisons, sequences of the KTN domain of E. coli KefC (EcKefC, P03819), AtKEA3, Arabidopsis KEA2 (AtKEA2, Q65272) and the first RCK domain of V. parahaemolyticus TrkA (VcTrkA, A0A072LGS4) were aligned using ClustalOmega and shaded according to amino acid identity (black) and similarity...”
- KtrAB, a new type of bacterial K(+)-uptake system from Vibrio alginolyticus
Nakamura, Journal of bacteriology 1998 - “...3365) and from KefC of K1-efflux channel from E. coli (P03819 in X56742, residues 399 to 620) (12), respectively. (B) KtrB Bbu, KtrB Ssp, KtrB Aae, KtrB Ehi,...”
- Analysis of phage Mu DNA transposition by whole-genome Escherichia coli tiling arrays reveals a complex relationship to distribution of target selection protein B, transcription and chromosome architectural elements
Ge, Journal of biosciences 2011 - “...3104517 1.83 nupG b2964 12 4449949 4450142 3.14 ytfR b4230 13 48080 48753 1.77 kefC b0047 13 94377 94642 3.08 murE b0086 14 500178 500563 1.77 gsk b0477 14 2443970 2444451 3.08 mepA b2328 15 2052323 2052876 1.77 amn b1982 15 2750846 2751039 3.08 smpA b2617...”
EC958_0181 glutathione-regulated potassium-efflux system protein KefC from Escherichia coli O25b:H4-ST131
43% identity, 98% coverage
A4W6F3 Glutathione-regulated potassium-efflux system protein KefC from Enterobacter sp. (strain 638)
45% identity, 96% coverage
S0046 K+ efflux antiporter from Shigella flexneri 2a str. 2457T
43% identity, 98% coverage
SEN_RS00445 glutathione-regulated potassium-efflux system protein KefC from Salmonella enterica subsp. enterica serovar Enteritidis str.
SPA0087 glutathione-regulated potassium-efflux system protein KefC from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150
45% identity, 93% coverage
Q8EGW0 Glutathione-gated K(+)-efflux system KefC from Shewanella oneidensis (strain ATCC 700550 / JCM 31522 / CIP 106686 / LMG 19005 / NCIMB 14063 / MR-1)
SO1481 glutathione-regulated potassium-efflux system protein from Shewanella oneidensis MR-1
42% identity, 94% coverage
lpg1897 glutathione-regulated potassium efflux system from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
40% identity, 95% coverage
- Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires' Disease Outbreak Isolates and Additional ST36 Strains
Mercante, PloS one 2016 - “...1 420,902 383,012 rpsJ/nusE (lpg0328) G34S T C 1 2,163,269 2,115,012 Glutathione-regulated potassium efflux system (lpg1897) L7P C A 1 2,442,338 2,394,082 Downstream of tutC response regulator (lpg2146) Unknown A G 1 2,539,995 2,491,740 Downstream of hypothetical protein (lpg2207) Unknown Insertion 2 3,125,898 3,077,599 Hypothetical protein...”
LHK_02848 Kef from Laribacter hongkongensis HLHK9
45% identity, 94% coverage
- Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
Lau, Cell & bioscience 2011 - “.../H + exchangers (LHK_02296, LHK_00707) while the other two code for the putative genes kef (LHK_02848) and kefB (LHK_02018). No CDS encoding putative homologue of monovalent cation/proton antiporter-1 (CPA1) or monovalent cation/proton antiporter-3 (CPA3) has been identified. Transporters of the monovalent cation/proton antiporter (CPA) superfamily support...”
- “...hydrogen influx LHK_02018 CV3326 NMB0209 - Glutathione-regulated potassium-efflux system protein kef Potassium efflux, hydrogen influx LHK_02848 - - NGO1774 Potassium-transporting ATPase, A subunit kdpA Potassium uptake LHK_01572 CV1599 - - Potassium-transporting ATPase, B subunit kdpB Potassium uptake LHK_01573 CV1598 - - Potassium-transporting ATPase, C subunit kdpC...”
VDA_003428 glutathione-regulated potassium-efflux system protein KefB from Photobacterium damselae subsp. damselae CIP 102761
43% identity, 96% coverage
8by2A / P03819 Structure of the k+/h+ exchanger kefc with gsh. (see paper)
46% identity, 90% coverage
- Ligands: potassium ion; adenosine monophosphate; glutathione; (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl(9z)-octadec-9-enoate (8by2A)
C5975_08860 glutathione-regulated potassium-efflux system protein KefC from Cronobacter sakazakii
44% identity, 95% coverage
- Draft genomes of Cronobacter sakazakii strains isolated from dried spices bring unique insights into the diversity of plant-associated strains
Jang, Standards in genomic sciences 2018 - “...(locus tag C5975_00275), p-hydroxybenzoic acid (locus tag C5975_07280), glutathione-regulated potassium (locus tag C5975_00475, C5975_00480, C5975_08855, C5975_08860, KefGFCB), RND efflux (C5975_02520, Transporter), proteins associated with heavy metal efflux of nickel/cobalt (C5975_13445, RcnB), cobalt/magnesium (C5975_08880, ApaG), and manganese ions (C5975_18840, MntP), sugar efflux (C5975_13720, SetB), and multidrug resistance...”
PP0713 potassium efflux transporter, putative from Pseudomonas putida KT2440
62% identity, 64% coverage
VF_0220 glutathione-regulated potassium-efflux system protein KefB from Aliivibrio fischeri ES114
VF_0220 potassium:proton antiporter from Vibrio fischeri ES114
42% identity, 97% coverage
SPO1424 monovalent cation:proton antiporter-2 (CPA2) family protein from Ruegeria pomeroyi DSS-3
42% identity, 93% coverage
- A novel class of sulfur-containing aminolipids widespread in marine roseobacters
Smith, The ISME journal 2021 - “...05K39 SPO1109 S Endonuclease exonuclease phosphatase 05NU0 SPO1287 S Glyoxalase bleomycin resistance protein dioxygenase 08EFD SPO1424 P Sodium hydrogen exchanger 08K1F SPO1437 I, Q Dehydrogenase 05D82 SPO1743 E Glutamate dehydrogenase 05S99 SPO1919 P Tellurite resistance protein 090P8 SPO1963 G Phosphoglycerate mutase 05N59 SPO2284 M Transglycosylase 01QFF...”
HI0911 glutathione-regulated potassium efflux system protein (kefC) from Haemophilus influenzae Rd KW20
38% identity, 91% coverage
glr1343 glutathione-regulated potassium efflux system protein KefC homolog from Gloeobacter violaceus PCC 7421
43% identity, 94% coverage
- Function and evolution of channels and transporters in photosynthetic membranes
Pfeil, Cellular and molecular life sciences : CMLS 2014 - “...also lie along the stem lineage of the KEA1 to KEA3 clade (that includes Gloeobacter glr1343 KefC), the KEA4 to KEA6 clade (that includes Ectocarpus CBJ26612), the CHX clade (not shown), or the clade of four cyanobacterial proteins (that includes Synechocystis NhaS3). A rooting along the...”
RTCIAT899_RS04005 monovalent cation:proton antiporter-2 (CPA2) family protein from Rhizobium tropici CIAT 899
41% identity, 89% coverage
- Dissecting the Acid Stress Response of Rhizobium tropici CIAT 899
Guerrero-Castro, Frontiers in microbiology 2018 - “...transporter Kup Foster, 2004 gshB RTCIAT899_RS01980 1.00662168 0.28522761 Glutathione synthase Riccillo et al., 2000 kefB/kefC RTCIAT899_RS04005 0.13325459 0.09956478 System protein KefB/KefC Clc RTCIAT899_RS10520 0.01091242 0.05697754 Chloride channel protein Accardi and Miller, 2004 Clc RTCIAT899_RS04900 0.00017649 0.31726611 ClC-type chloride channel Clc RTCIAT899_RS14650 0.02874732 0.2712195 ClC-type chloride channel...”
Atu1693 glutathione-regulated potassium-efflux system protein from Agrobacterium tumefaciens str. C58 (Cereon)
A9CIR7 Glutathione-regulated potassium-efflux system protein from Agrobacterium fabrum (strain C58 / ATCC 33970)
39% identity, 92% coverage
NP_384912 PROBABLE GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM TRANSMEMBRANE PROTEIN from Sinorhizobium meliloti 1021
41% identity, 92% coverage
- 'Ca. Liberibacter asiaticus' proteins orthologous with pSymA-encoded proteins of Sinorhizobium meliloti: hypothetical roles in plant host interaction
Kuykendall, PloS one 2012 - “...acid sequence (E=1e-134) than is NP_436118.1 and its ortholog encoded by the S. meliloti chromosome, NP_384912 (E=1e-22). Bioinformatic analyses revealed that YP_003065337 is a potassium efflux protein. Control of intracellular pH is ascribed in its domain architecture. SMART analysis of YP_003065337 confidently predicts two domains as...”
- “...transporter NP_436297.2 YP_003064775 3E-30 333 44 ******** NA Na+/H+ antiporter NP_436118.1 YP_003065337 1E-134 605 62 NP_384912 1E-22 Potassium efflux protein NP_436237.1 YP_003065245 0E+00 624 67 NP_384938 0E+00 put potassium uptake protein Signal Transduction NP_436498.1 YP_003064826 3E-16 239 49 NP_384498 6E-22 signal transduction histidine kinase NP_436497.1 YP_003065306...”
CCNA_00204 glutathione-regulated potassium-efflux system protein kefB from Caulobacter crescentus NA1000
43% identity, 93% coverage
- Regulation of potassium uptake in <i>Caulobacter crescentus</i>
Quintero-Yanes, Journal of bacteriology 2024 (secret) - Untargeted metabolomics links glutathione to bacterial cell cycle progression
Hartl, Nature metabolism 2020 - “...I and II harboured a non-synonymous mutation (Q92L) at a highly conserved residue of KefB (CCNA_00204, Supplementary Fig. 19a, b ), a predicted glutathione-dependent potassium efflux system ( Fig. 5h ). Suppressor V had a 720 bp deletion at the kefG locus (CCNA_00205, Supplementary Fig. 19c...”
- “...(M.R. Alley) digested with SpeI/EcoRI. For construction of pNPTStet- kefB regions up- and downstream of CCNA_00204 were PCR-amplified from C. crescentus NA1000 gDNA with primer pairs 12527/12917 and 12525/12918, respectively, the resulting PCR products were joined by SOE-PCR using primer pair 12525/12527 and cloned in pNPTStet...”
SMb21555 putative potassium-efflux protein from Sinorhizobium meliloti 1021
39% identity, 88% coverage
HD0614 glutathione-regulated potassium-efflux system protein from Haemophilus ducreyi 35000HP
38% identity, 90% coverage
GSU2759 potassium efflux system protein from Geobacter sulfurreducens PCA
40% identity, 92% coverage
XP_021717525 K(+) efflux antiporter 2, chloroplastic-like from Chenopodium quinoa
43% identity, 42% coverage
- Salinity Effects on Guard Cell Proteome in Chenopodium quinoa
Rasouli, International journal of molecular sciences 2021 - “...import receptor subunit TOM40-1-like Porin XP_021758463 mitochondrial outer membrane protein porin of 34 kDa Porin XP_021717525 K + efflux antiporter 2, chloroplastic-like Potassium XP_021762166 probable voltage-gated potassium channel subunit beta Potassium XP_021753247 monosaccharide-sensing protein 2-like Sugar XP_021726328 sugar carrier protein C-like Sugar XP_021757156 plastidic glucose transporter...”
KEA1_ARATH / Q9ZTZ7 K(+) efflux antiporter 1, chloroplastic; AtKEA1 from Arabidopsis thaliana (Mouse-ear cress) (see 13 papers)
TC 2.A.37.1.4 / Q9ZTZ7 K+ efflux antiporter 1, chloroplastic (AtKEA1) from Arabidopsis thaliana (see 7 papers)
NP_171684 K+ efflux antiporter 1 from Arabidopsis thaliana
AT1G01790 KEA1 (K EFFLUX ANTIPORTER 1); potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
42% identity, 43% coverage
- function: Electroneutral K(+)/H(+) efflux antiporter involved in chloroplastic K(+) homeostasis and osmotic adjustment, especially during plastid division and thylakoid membrane formation (PubMed:24278440, PubMed:27443603, PubMed:31296940, PubMed:33111995). Collaboratively with KEA2, adjusts alkaline stromal pH upon light to dark transitions in plastids (PubMed:33111995). Together with KEA2, critical for chloroplast development, including chloroplast RNA- metabolism (e.g. rRNA maturation, polysome loading and RNA-protein interactions) and plastid gene expression (PGE), ion homeostasis, and photosynthesis (PubMed:27443603, PubMed:34235544). Contributes, during early seedling development, to the regulation of photosynthesis and abscisic acid- (ABA-) mediated primary root growth in a sucrose- dependent manner (PubMed:35193734). Involved in the regulation of reactive oxygen and nitrogen species (ROS and RNS) metabolism (PubMed:33485149). Required in roots for rapid hyperosmotic-induced Ca(2+) responses and for osmo-sensory potentiation in hyperosmotic conditions (PubMed:27528686). May counteract resilience to drought and salt stress, involving photorespiratory pathway and stomata closure (PubMed:33485149, PubMed:34235544).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:24794527). Disrupted K(+)/H(+) efflux antiporter in the kea1 kea2 double mutant (PubMed:33111995). Mutants kea1 kea2 display strong growth retardation along with pale green leaves associated with a delayed formation of chloroplast pigments and electron transport complexes, as well as maturation defects of the plastid ribosomal RNAs and hampered RNA- protein interactions (PubMed:24794527, PubMed:27443603, PubMed:34235544). Double mutant kea1 kea2 exhibits a drastic photosystem II (PSII) quantum yield recovery under moderate salt stress (PubMed:34235544). Impaired rapid hyperosmotic-induced Ca(2+) responses in kea1 kea2 (PubMed:27528686). K(+)-induced alkalinization of plastid stroma is suppressed in kea1 kea2 mutants, as well as delayed rate of decay to neutral pH in the dark, but normal light-stimulated alkalinization of the stroma (PubMed:33111995). In addition, reduced primary root length is observed in the kea1 kea2 double mutant in the absence of sucrose, associated with lower abscisic acid (ABA) accumulation and impaired photosynthesis; these phenotypes are partially restored in the presence of moderate NaCl treatment (75 mM) (PubMed:33485149, PubMed:35193734). Plants kea1 kea2 have slightly higher K(+) levels and altered reactive oxygen and nitrogen species (ROS and RNS) metabolism, but enhanced resilience to drought stress due to an increased photorespiratory pathway and stomata closure (PubMed:33485149). Triple mutants kea1 kea2 kea3 are extremely stunted in size with entirely pale leaves and died before steeing seeds (PubMed:24794527). - substrates: H+, K+
tcdb comment: It localizes to the chloroplast inner envelope membrane and is essential for normal osmoregulation (Kunz et al. 2014). It has an even number of TMSs, probably 14, with N- and C-terminal hydrophilic regulatory domains (Bölter et al. 2019) - Quantitative Phosphoproteomics of cipk3/9/23/26 Mutant and Wild Type in Arabidopsis thaliana
Yin, Genes 2021 - “...282 1.214 Probable aquaporin PIP2-6 S(1)QLHELHA A0A1P8AU30 30 1.27 Cationic amino acid transporter 9 S(0.012)KS(0.986)LPPPS(0.001)S(0.001)QT(0.001)AVR Q9ZTZ7 120 1.733 K(+) efflux antiporter 1, chloroplastic IGES(0.002)S(0.013)ES(0.864)S(0.121)DETEATDLK Q8W4J2 527 1.374 Mitogen-activated protein kinase 16 T(0.237)QPCKS(0.763)NRGDEDCATAAEGPSR Q94A06 27 1.235 Mitogen-activated protein kinase kinase 1 FLT(0.096)QS(0.9)GT(0.005)FKDGDLR Q9C9U4 530 1.742 Mitogen-activated protein...”
- Evidence for partial functional overlap of KEA and MSL transport proteins in the chloroplast inner envelope of Arabidopsis thaliana.
Völkner, FEBS letters 2024 (PubMed)- GeneRIF: Evidence for partial functional overlap of KEA and MSL transport proteins in the chloroplast inner envelope of Arabidopsis thaliana.
- Envelope K+/H+ Antiporters AtKEA1 and AtKEA2 Function in Plastid Development.
Aranda-Sicilia, Plant physiology 2016 - GeneRIF: Results show that envelope K+/H+ Antiporters AtKEA1 and AtKEA2 transporters in specific microdomains of the inner envelope link local osmotic, ionic, and pH homeostasis to plastid division and thylakoid membrane formation.
- Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters.
Stephan, Proceedings of the National Academy of Sciences of the United States of America 2016 - GeneRIF: Double mutations in the plastid K(+) exchange antiporter (KEA) transporters kea1kea2 and a single mutation that does not visibly affect chloroplast structure, kea3, impaired the rapid hyperosmotic-induced Ca(2+) responses.
- Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis.
Kunz, Proceedings of the National Academy of Sciences of the United States of America 2014 - GeneRIF: role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis
- Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts
Izumi, eLife 2024 - “...as follows. A genomic fragment containing the promoter region and full-length coding region of KEA1 (At1g01790) was amplified from Col-0 genomic DNA by PCR using the primers KEA1_F ( AGGAACCAATTCAGTCGACTCATGATCATAACAAGTCTC ) and KEA1_R ( AAAGCTGGGTCTAGATATCCGATTACGACTGTGCCTCCTTC ), cloned into pENTR1A (Invitrogen) using NEBuilder HiFi DNA Assembly master...”
- “...Gene ( A. thaliana ) ATPC1 TAIR AT4G04640 Gene ( A. thaliana ) KEA1 TAIR AT1G01790 Genetic reagent ( A. thaliana ) atg5-1 ABRC SAIL_129_B07 Genetic reagent ( A. thaliana ) atg7-2 ABRC GABI_655B06 Genetic reagent ( A. thaliana ) atg2-1 ABRC SALK_076727 Genetic reagent (...”
- A metabolic, phylogenomic and environmental atlas of diatom plastid transporters from the model species Phaeodactylum
Liu, Frontiers in plant science 2022 - “...Figure1C ), such as probable homologues of the KEA K + /H + antiporters family (AT1G01790, AT4G04850 and AT1G01790). The P. tricornutum homologues of a thylakoid ADP/ATP carrier protein (AT5G01500), implicated in both ATP/ADP and 3-phosphoadenosine 5-phosphosulfate (PAPS) transport across the A. thaliana plastid envelope (...”
- Chloroplast pH Homeostasis for the Regulation of Photosynthesis
Trinh, Frontiers in plant science 2022 - “...transporters and protein factors regulate chloroplast stromal pH. Locus Protein Subcellular localization Proposed function(s) References At1g01790 KEA1 Chloroplast envelope membrane K + /H + specific antiporters Regulation of K + -induced stromal alkalization upon dark-to-light transition. Regulation of neutralization of stromal pH upon light-to-dark transition. Aranda-Sicilia...”
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “..., and G. barbadense . ID NAME Chr. Length Mw(kDa) pI GRAVY Subcellular localization Location AT1G01790 ATKEA1 1 1193 128.034 5.22 0.08 Chloroplast 284350291203 AT4G00630 ATKEA2 4 1185 127.605 5.11 0.086 Chloroplast 261246268097 AT4G04850 ATKEA3 4 776 83.7907 5.53 0.369 Chloroplast 24526642457767 AT2G19600 ATKEA4 2 592...”
- “...Sorghum bicolor ID Name 5 Triticum aestivum ID Name 6 Glycine max ID Name 7 AT1G01790 ATKEA1 LOC_Os04g58620.1 OsKEA1 GRMZM2G093643_P01 ZmKEA1 Potri.002G157200.1.p PtKEA1 Sobic.006G271800.1.p SbKEA1 Traes_2AL_A2CBDB5F7.1 TaKEA1 Glyma.03G014000.1.p GmKEA1 AT4G00630 ATKEA2 LOC_Os12g42300.1 OsKEA2 GRMZM2G009715_P01 ZmKEA2 Potri.014G080800.1.p PtKEA2 Sobic.008G173800.1.p SbKEA2 Traes_2BL_BA71DD685.1 TaKEA2 Glyma.07G073700.1.p GmKEA2 AT4G04850 ATKEA3 LOC_Os06g36590.1...”
- Loss of inner-envelope K+/H+ exchangers impairs plastid rRNA maturation and gene expression
DeTar, The Plant cell 2021 - “...Supplemental Dataset S6 . Accession numbers These genetic loci were important to this project: KEA1 (AT1G01790), KEA2 (AT4G00630), GLK1 (AT2G20570), GLK2 (AT5G44190), GUN1 (AT2G31400), VAR2 (AT2G30950), RAP (AT2G31890), RPS5 (AT2G33800), PAC (AT2G48120), PHYA (AT1G09570), PHYB (AT2G18790), LHCB1.2 (AT1G29910), LHCB2.2 (AT2G05070), SAND (AT2G28390), and TIP2 (AT3G26520). For...”
- Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria
Møller, Biomolecules 2020 - “...Proton Antiporter-2 (CPA2) Family. At5g41610 (Cation/H + antiporter 18) was predicted but not found, while At1g01790 (K + efflux antiporter 1, chloroplastic) was found but not predicted. All of these transporters could help the mitochondria regulate the K + concentration in the matrix. Metal ionstwo members...”
- Dual lysine and N-terminal acetyltransferases reveal the complexity underpinning protein acetylation
Bienvenut, Molecular systems biology 2020 - “...n.i . + 8 AT3G54050 Plastid F16P1 61 (Nt); 323 (K) 0.32 0.97 + 1* AT1G01790 Plastid KEA1 168 (K) n.i . 0.02 + 2* AT2G05310 Plastid Q9SJ31 62 (K) n.i . 0.20 + 3* AT5G01600 Plastid FRI1 134 (K) n.i . 0.65 + * AT1G06680...”
- Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity
Bouchnak, Molecular & cellular proteomics : MCP 2019 - “...(see supplemental Table S5 , lane 1220). On the other hand, its close homologues, KEA1 (At1g01790) and KEA2 (At4g00630), known envelope transporters ( 67 , 68 ), have EF values of 8 and 15 respectively (see supplemental Table S5 , lanes 286287). We previously provided data...”
- More
XP_006339534 K(+) efflux antiporter 2, chloroplastic from Solanum tuberosum
43% identity, 42% coverage
LOC101246664 K(+) efflux antiporter 2, chloroplastic from Solanum lycopersicum
42% identity, 42% coverage
KEA2_ARATH / O65272 K(+) efflux antiporter 2, chloroplastic; AtKEA2 from Arabidopsis thaliana (Mouse-ear cress) (see 13 papers)
TC 2.A.37.1.7 / O65272 Chloroplastic K+ efflux antiporter 2, AtKEA2. Cation preference: from Arabidopsis thaliana (see 5 papers)
NP_191972 K+ efflux antiporter 2 from Arabidopsis thaliana
AT4G00630 KEA2; potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
42% identity, 43% coverage
- function: Electroneutral K(+)/H(+) efflux antiporter modulating monovalent cation and pH homeostasis in plastids, especially during plastid division and thylakoid membrane formation (PubMed:22551943, PubMed:24278440, PubMed:27443603, PubMed:31296940, PubMed:33111995). Transports K(+) and Cs(+) preferentially relative to Na(+) or Li(+) (PubMed:22551943). May function in osmotic adjustment (PubMed:24278440). Collaboratively with KEA1, adjusts alkaline stromal pH upon light to dark transitions in plastids (PubMed:33111995). Together with KEA1, critical for chloroplast development, including chloroplast RNA-metabolism (e.g. rRNA maturation, polysome loading and RNA-protein interactions) and plastid gene expression (PGE), ion homeostasis, and photosynthesis (PubMed:27443603, PubMed:34235544). Contributes, during early seedling development, to the regulation of photosynthesis and abscisic acid- (ABA-) mediated primary root growth in a sucrose-dependent manner (PubMed:35193734). Involved in the regulation of reactive oxygen and nitrogen species (ROS and RNS) metabolism (PubMed:33485149). Required in roots for rapid hyperosmotic- induced Ca(2+) responses and for osmo-sensory potentiation in hyperosmotic conditions (PubMed:27528686). May counteract resilience to drought and salt stress, involving photorespiratory pathway and stomata closure (PubMed:33485149, PubMed:34235544).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:24794527). Disrupted K(+)/H(+) efflux antiporter in the kea1 kea2 double mutant (PubMed:33111995). Double mutants kea1 kea2 display strong growth retardation along with pale green leaves associated with a delayed formation of chloroplast pigments and electron transport complexes, as well as maturation defects of the plastid ribosomal RNAs and hampered RNA-protein interactions (PubMed:24794527, PubMed:27443603, PubMed:34235544). The double mutant kea1 kea2 exhibits a drastic photosystem II (PSII) quantum yield recovery under moderate salt stress (PubMed:34235544). Impaired rapid hyperosmotic-induced Ca(2+) responses in kea1 kea2 (PubMed:27528686). K(+)-induced alkalinization of plastid stroma is suppressed in kea1 kea2 mutants, as well as delayed rate of decay to neutral pH in the dark, but normal light-stimulated alkalinization of the stroma (PubMed:33111995). In addition, reduced primary root length is observed in the kea1 kea2 double mutant in the absence of sucrose, associated with lower abscisic acid (ABA) accumulation and impaired photosynthesis; these phenotypes are partially restored in the presence of moderate NaCl treatment (75 mM) (PubMed:33485149, PubMed:35193734). Plants kea1 kea2 have slightly higher K(+) levels and altered reactive oxygen and nitrogen species (ROS and RNS) metabolism, but enhanced resilience to drought stress due to an increased photorespiratory pathway and stomata closure (PubMed:33485149). Triple mutants kea1 kea2 kea3 are extremely stunted in size with entirely pale leaves and died before steeing seeds (PubMed:24794527). - substrates: Cs+, K+, Li+, Na+
tcdb comment: K+ > Cs+ > Li+ > Na+ (Aranda-Sicilia et al. 2012). The N-terminal hydrophilic domain show sequence similarity to members of the MPA1-C family (8.A.3.1.2). Localizes to the chloroplast inner envelope membrane and plays a role in chloroplast integrity (Kunz et al. 2014). It is up-regulated under salt stress conditions (Yang et al. 2018Yang et al. 2018) - Physiological and Transcriptional Responses of Apocynum venetum to Salt Stress at the Seed Germination Stage
Li, International journal of molecular sciences 2023 - “...chloroplastic 8.69 RAN1 Q9S7J8 Copper-transporting ATPase RAN1 4.28 ERD4 A9LIW2 CSC1-like protein ERD4 3.81 KEA2 O65272 K(+) efflux antiporter 2, chloroplastic 5.20 MOT1 Q9SL95 Molybdate transporter 1 2.18 NEW1 Q08972 [NU+] prion formation protein 1 9.03 OEP16 Q41050 Outer envelope pore protein 16, chloroplastic 3.58 OEP162...”
- Envelope K+/H+ Antiporters AtKEA1 and AtKEA2 Function in Plastid Development.
Aranda-Sicilia, Plant physiology 2016 - GeneRIF: Results show that envelope K+/H+ Antiporters AtKEA1 and AtKEA2 transporters in specific microdomains of the inner envelope link local osmotic, ionic, and pH homeostasis to plastid division and thylakoid membrane formation.
- Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters.
Stephan, Proceedings of the National Academy of Sciences of the United States of America 2016 - GeneRIF: Double mutations in the plastid K(+) exchange antiporter (KEA) transporters kea1kea2 and a single mutation that does not visibly affect chloroplast structure, kea3, impaired the rapid hyperosmotic-induced Ca(2+) responses.
- Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis.
Kunz, Proceedings of the National Academy of Sciences of the United States of America 2014 - GeneRIF: role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis
- Arabidopsis KEA2, a homolog of bacterial KefC, encodes a K(+)/H(+) antiporter with a chloroplast transit peptide.
Aranda-Sicilia, Biochimica et biophysica acta 2012 (PubMed)- GeneRIF: AtKEA2 represents the founding member of a novel group of eukaryote K(+)/H(+) antiporters that modulate monovalent cation and pH homeostasis in plant chloroplasts or plastids
- Phosphoproteomic analysis of distylous Turnera subulata identifies pathways related to endoreduplication that correlate with reciprocal herkogamy
Henning, American journal of botany 2024 - “...0.0467 1.094 N/A Y AT1G24300 GYF domaincontaining protein. Tsubulata_007186 Y 1.74 0.0213 1.138 N/A N AT4G00630 Encodes a K(+)/H(+) antiporter that modulates monovalent cation and pH homeostasis in plant chloroplasts or plastids. 3.3 0.0083 Tsubulata_007753 N N/A N/A 0.001 Lmorph Y AT3G33530 Transducin family protein/WD40 repeat...”
- Identification of QTNs, QTN-by-environment interactions, and their candidate genes for salt tolerance related traits in soybean
Chen, BMC plant biology 2024 - “...phytochrome-associated protein 2 FWR V snp11062 3 1,445,425 9.66 -0.93 3.46 2.22 2.17E-10 SIG Glyma03g01603 AT4G00630 KEA2 K+efflux antiporter 2 FWR VI snp11062 3 1,445,425 12.68 -2.16 0.20 0.47 2.08E-13 SIG Glyma03g01603 AT4G00630 KEA2 K+efflux antiporter 2 FWR IV Gm07:17,751,732 7 17,751,732 7.67 0.23 -9.31 3.29...”
- 3D organization of regulatory elements for transcriptional regulation in Arabidopsis
Deng, Genome biology 2023 - “...Table S6). For instance, an SNP regulating Arabidopsis flowering time was mapped to intron of At4G00630, 52kb downstream of MED12 , a transcription regulator [ 73 ], and 5kb upstream of FRIGIDA , a regulator of vegetative phase change [ 74 ]. We have now detected...”
- Chloroplast pH Homeostasis for the Regulation of Photosynthesis
Trinh, Frontiers in plant science 2022 - “...light-to-dark transition. Aranda-Sicilia et al., 2012 Kunz et al., 2014 Aranda Sicilia et al., 2021 At4g00630 KEA2 Chloroplast envelope membrane (distinct spots) * At4g13590 PAM71-HL BICAT2 Chloroplast envelope membrane Putative Ca 2+/ H + antiporter Mn 2+ , Ca 2+ , and pH homeostasis in chloroplasts...”
- Genotype-dependent contribution of CBF transcription factors to long-term acclimation to high light and cool temperature
Baker, Plant, cell & environment 2022 - “...as chloroplast glucose6 phosphate/phosphate translocator GPT2 (AT1G61800), chloroplast envelope K + /H + antiporter KEA2 (AT4G00630), phosphofructokinase (AT1G76550), cytosolic fumarase (AT5G76550), ferritin (AT2G30400/AT3G56090) and pyridoxal phosphate synthase protein (AT5G01410). For both ecotypes, genes preferentially induced under HLC were enriched for those that had also been induced...”
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “...Mw(kDa) pI GRAVY Subcellular localization Location AT1G01790 ATKEA1 1 1193 128.034 5.22 0.08 Chloroplast 284350291203 AT4G00630 ATKEA2 4 1185 127.605 5.11 0.086 Chloroplast 261246268097 AT4G04850 ATKEA3 4 776 83.7907 5.53 0.369 Chloroplast 24526642457767 AT2G19600 ATKEA4 2 592 64.2494 5.91 0.589 Plasma membrane 84789708483854 AT5G51710 ATKEA5 5...”
- “...7 AT1G01790 ATKEA1 LOC_Os04g58620.1 OsKEA1 GRMZM2G093643_P01 ZmKEA1 Potri.002G157200.1.p PtKEA1 Sobic.006G271800.1.p SbKEA1 Traes_2AL_A2CBDB5F7.1 TaKEA1 Glyma.03G014000.1.p GmKEA1 AT4G00630 ATKEA2 LOC_Os12g42300.1 OsKEA2 GRMZM2G009715_P01 ZmKEA2 Potri.014G080800.1.p PtKEA2 Sobic.008G173800.1.p SbKEA2 Traes_2BL_BA71DD685.1 TaKEA2 Glyma.07G073700.1.p GmKEA2 AT4G04850 ATKEA3 LOC_Os06g36590.1 OsKEA3 GRMZM2G169114_P01 ZmKEA3 Potri.009G080800.2.p PtKEA3 Sobic.010G168900.1.p SbKEA3 Traes_2DL_053E73CFE.1 TaKEA3 Glyma.09G262000.1.p GmKEA3 AT2G19600 ATKEA4 LOC_Os03g03590.1...”
- Loss of inner-envelope K+/H+ exchangers impairs plastid rRNA maturation and gene expression
DeTar, The Plant cell 2021 - “...S6 . Accession numbers These genetic loci were important to this project: KEA1 (AT1G01790), KEA2 (AT4G00630), GLK1 (AT2G20570), GLK2 (AT5G44190), GUN1 (AT2G31400), VAR2 (AT2G30950), RAP (AT2G31890), RPS5 (AT2G33800), PAC (AT2G48120), PHYA (AT1G09570), PHYB (AT2G18790), LHCB1.2 (AT1G29910), LHCB2.2 (AT2G05070), SAND (AT2G28390), and TIP2 (AT3G26520). For information on...”
- Unraveling Hidden Components of the Chloroplast Envelope Proteome: Opportunities and Limits of Better MS Sensitivity
Bouchnak, Molecular & cellular proteomics : MCP 2019 - “...S5 , lane 1220). On the other hand, its close homologues, KEA1 (At1g01790) and KEA2 (At4g00630), known envelope transporters ( 67 , 68 ), have EF values of 8 and 15 respectively (see supplemental Table S5 , lanes 286287). We previously provided data ( 12 )...”
- More
OTBS_1539 Glutathione-regulated potassium-efflux system protein from Orientia tsutsugamushi Boryong
35% identity, 92% coverage
H375_7130 monovalent cation:proton antiporter-2 (CPA2) family protein from Rickettsia prowazekii str. Breinl
35% identity, 91% coverage
RT0798 glutathione-regulated potassium-efflux system from Rickettsia typhi str. wilmington
35% identity, 91% coverage
SPISAL_01180 cation:proton antiporter from Spiribacter salinus M19-40
38% identity, 80% coverage
Q2QM48 Os12g0617800 protein from Oryza sativa subsp. japonica
36% identity, 72% coverage
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...(EcKefC, P03819), AtKEA3, Arabidopsis KEA2 (AtKEA2, Q65272) and homologous AtKEA3 sequences from Oryza sativa (O.s., Q2QM48), Physcomitrella patens (P.p., A9SSR0) and Chlamydomonas reinhardtii (C.r., A8ISE8) by using ClustalOmega ( http://www.ebi.ac.uk/Tools/msa/clustalo/ ). For the homologous KEA3 sequences, an identity matrix was also calculated for the KTN domain...”
KEA3_ARATH / Q9M0Z3 K(+) efflux antiporter 3, chloroplastic; AtKEA3; Protein DISTURBED PROTON GRADIENT REGULATION from Arabidopsis thaliana (Mouse-ear cress) (see 13 papers)
TC 2.A.37.1.6 / Q9M0Z3 K+ efflux - K+:H+ antiporter 3 (AtKEA3) of 776 aas and 13 TMSs from Arabidopsis thaliana (see 4 papers)
NP_001190675 K+ efflux antiporter 3 from Arabidopsis thaliana
36% identity, 71% coverage
- function: [Isoform 1]: Electroneutral K(+)/H(+) efflux antiporter assuring proton efflux from the thylakoid lumen to the plastid stroma, thus increasing the membrane potential at the expense of the proton gradient (delta pH) component of the proton motive force (PMF) (PubMed:24278440, PubMed:25451040, PubMed:27783435, PubMed:31053658, PubMed:31296940, PubMed:31427465). Promotes photosynthesis and growth in conditions where the chloroplast (cp)ATP synthase activity is low (e.g. cgl160 mutant background) by reducing the pH gradient across the thylakoid membrane (PubMed:32041909). Accelerates photosynthetic acclimation in fluctuating light environments by modulating two components of the proton motive force, the proton gradient and the electric potential (delta Psi) (PubMed:25451040, PubMed:27335350, PubMed:27783435, PubMed:31053658, PubMed:31427465). Promotes the relaxation of photoprotective energy-dependent non-photochemical quenching (NPQ) after transitions from high to low light, thus enhancing photosystem II (PSII) quantum efficiency in fluctuating light (PubMed:27335350). On transition from high to low light, slows down photoprotection by dissipating the pH gradient across the thylakoid membrane (PubMed:31201341). During photosynthetic response on transition from dark to low light, involved in a sequential mechanism of adaptation; VCCN1 and CLCe first trigger the activation of photoprotection, which is later down-regulated by KEA3 to a low steady state, while adjusting electron transport (PubMed:31201341). Together with the chloroplast NADH dehydrogenase-like (NDH) complex, maximizes photosynthesis efficiency after a long dark adaptation (PubMed:32978277). Required in roots for rapid hyperosmotic-induced Ca(2+) responses and for osmo-sensory potentiation in hyperosmotic conditions (PubMed:27528686).
function: [Isoform 3]: Low K(+)/H(+) efflux antiporter activity.
function: [Isoform 4]: Low K(+)/H(+) efflux antiporter activity (PubMed:31296940). Promotes non-photochemical quenching (NPQ) in high light conditions (PubMed:27335350).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:24794527, PubMed:25451040, PubMed:31201341). Impaired rapid hyperosmotic-induced Ca(2+) responses in kea3 (PubMed:25451040, PubMed:27528686). Altered non-photochemical quenching (NPQ) and photosystem II (PSII) quantum efficiency after transitions from high to low light leading to a reduced growth rate (PubMed:27335350, PubMed:31053658, PubMed:31427465). Transiently increased NPQ upon transition from dark to low light (PubMed:31201341). Slightly faster NPQ induction on transition from low to high light (PubMed:31201341). Delayed NPQ relax on transition from high to low light (PubMed:31201341). In contrast to dpgr, the mutant kea3 has a high-NPQ phenotype during steady state in ambient air (PubMed:27783435). The kea3 pgr5 double mutant combines the phenotypes of the single mutants in NPQ induction upon overnight dark adaptation (PubMed:32978277). The double mutant crr2 kea3 enhances the kea3 single mutant phenotypes, and delays induction of CO(2) fixation after overnight dark adaptation (PubMed:32978277). Triple mutants kea1 kea2 kea3 are extremely stunted in size with entirely pale leaves and died before steeing seeds (PubMed:24794527). Lower photosynthetic performance in dark-adapted clce kea3 double mutant, as well as in the clce kea3 vccn1 triple mutant (PubMed:31201341). Altered NPQ upon transition from dark to low light in clce kea3 and kea3 vccn1 double mutants and in the clce kea3 vccn1 triple mutant (PubMed:31201341). Strong growth penalty in the cgl160 kea3 double mutant associated with a low lumenal pH leading to a reduced photosynthetic capacity (PubMed:32041909). - substrates: H+, K+
tcdb comment: Localizes to the thylakoid membrane of the chloroplast. It modulates pmf partitioning through H+ efflux from the lumen, which is critical for photosynthetic acclimation after transitions from high to low light intensity (Armbruster et al. 2014; Kunz et al. 2014). It has an even number of TMSs, probably 14 (Bölter et al. 2019), with hydrophilic N- and C-terminal regulatory domains - Modification of Activity of the Thylakoid H+/K+ Antiporter KEA3 Disturbs ∆pH-Dependent Regulation of Photosynthesis.
Wang, Plant physiology 2019 - GeneRIF: Modification of Activity of the Thylakoid H(+)/K(+) Antiporter KEA3 Disturbs pH-Dependent Regulation of Photosynthesis.
- Fine-tuned regulation of the K+ /H+ antiporter KEA3 is required to optimize photosynthesis during induction.
Wang, The Plant journal : for cell and molecular biology 2017 (PubMed)- GeneRIF: Findings suggest that fine regulation of K+ efflux antiporter KEA3 activity is necessary for optimizing photosynthesis.
- Regulation and Levels of the Thylakoid K+/H+ Antiporter KEA3 Shape the Dynamic Response of Photosynthesis in Fluctuating Light.
Armbruster, Plant & cell physiology 2016 - GeneRIF: KEA3 activity is regulated by a mechanism involving its lumen-localized C-terminus, which lowers KEA3 activity in high light.
- Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters.
Stephan, Proceedings of the National Academy of Sciences of the United States of America 2016 - GeneRIF: Double mutations in the plastid K(+) exchange antiporter (KEA) transporters kea1kea2 and a single mutation that does not visibly affect chloroplast structure, kea3, impaired the rapid hyperosmotic-induced Ca(2+) responses.
- Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis.
Kunz, Proceedings of the National Academy of Sciences of the United States of America 2014 - GeneRIF: role of inner envelope KEA1 and KEA2 and thylakoid KEA3 transporters in chloroplast osmoregulation, integrity, and ion and pH homeostasis
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments.
Armbruster, Nature communications 2014 - GeneRIF: KEA3 is critical for high photosynthetic efficiency under fluctuating light.
- Proteomic Insight into the Response of Arabidopsis Chloroplasts to Darkness
Wang, PloS one 2016 - “...Inorganic phosphate transporter 2-1(PHT2;1) Q38954 AT3G26570 IE 2.480.06 46 K + efflux antiporter 3 (KEA3) Q9M0Z3 AT4G04850 T 1.440.06 Signaling (5) 47 Rhodanese/Cell cycle control phosphatase superfamily protein 9 (Str9) O48529 AT2G42220 S 1.480.08 48 Rhodanese/cell cycle control phosphatase superfamily protein F4J9G2 AT3G59780 T 1.490.02 49...”
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...http://www.cbs.dtu.dk/services/ChloroP ) 36 . For sequence comparisons, homologues of the Arabidopsis KEA3 protein (AtKEA3, Uniprot: Q9M0Z3) were identified by BLAST. Percent identity matrices were calculated for the CPA2 (Pfam00999) domains of E. coli NhaA (EcNhaA, P13738), T. thermophilus NapA (TtNapA, Q72IM4), E. coli KefC (EcKefC, P03819),...”
NMB0209 glutathione-regulated potassium-efflux system protein from Neisseria meningitidis MC58
34% identity, 83% coverage
XP_006359366 K(+) efflux antiporter 3, chloroplastic from Solanum tuberosum
36% identity, 57% coverage
CCNA_03611 glutathione-regulated potassium-efflux system protein kefC from Caulobacter crescentus NA1000
38% identity, 89% coverage
- Regulation of potassium uptake in <i>Caulobacter crescentus</i>
Quintero-Yanes, Journal of bacteriology 2024 (secret) - Untargeted metabolomics links glutathione to bacterial cell cycle progression
Hartl, Nature metabolism 2020 - “...regulation 50 . Like E. coli , C. crescentus encodes two paralogs, kefB and kefC (CCNA_03611). In E. coli , glutathione represses Kef transporters by binding to their C-terminal KTN domains, whereas various electrophiles produce glutathione-derivatives that activate the system and compete with glutathione for binding...”
- “...crescentus strain AKS451 as a template for PCR. Plasmid pQF- kefC was constructed by PCR-amplifying CCNA_03611 ( kefC ) from C. crescentus NA1000 gDNA with primers 13892/13893, digestion of the PCR product with SpeI/KpnI and cloning into pQF digested with the same enzymes, adding a C-terminal...”
NGO1774 putative potassium-efflux system protein from Neisseria gonorrhoeae FA 1090
35% identity, 82% coverage
AT4G04850 KEA3; potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
37% identity, 76% coverage
- Diatoms exhibit dynamic chloroplast calcium signals in response to high light and oxidative stress
Flori, Plant physiology 2024 - “...to the work described in this paper: CAS Gramene: AT5G23060 CAS Araport: AT5G23060 KEA3 Gramene: AT4G04850 KEA3 Araport: AT4G04850 PGR5 Gramene: AT2G05620 PGR5 Araport: AT2G05620 STN8 Gramene: AT5G01920 STN8 Araport: AT5G01920 application CHEBI: CHEBI:33232 npq4 Gramene: AT1G44575 npq4 Araport: AT1G44575 NADPH CHEBI: CHEBI:16474 References Allorent G...”
- Low-CO2-inducible bestrophins outside the pyrenoid sustain high photosynthetic efficacy in diatoms
Nigishi, Plant physiology 2024 - “...paper: BST1 Gramene: AT5G65090 BST1 Araport: AT5G65090 SDS Gramene: AT1G14750 SDS Araport: AT1G14750 KEA3 Gramene: AT4G04850 KEA3 Araport: AT4G04850 References Adler L , Lau CS , Shaikh KM , Maldegem K , Payne-Dwyer AL , Lefoulon C , Girr P , Atkinson N , Barrett J...”
- Transcriptome Analysis Reveals Genes Responsive to Three Low-Temperature Treatments in <i>Arabidopsis thaliana</i>
Ruiz-Aguilar, Plants (Basel, Switzerland) 2024 - “...type TF with transmembrane domains 7 At5g27970 At5g27970 7.4 6.5 ARM repeat superfamily protein 8 At4g04850 KEA3 7.4 5.9 K(+) efflux antiporter 3 No. ID Gene Protein 0 C 10 C Description 1 At1g34260 FAB1D 8.5 5.6 Putative 1-phosphatidylinositol-3-phosphate 5-kinase 2 At1g30620 MUR4 8.4 5.6 UDP-arabinose...”
- Arabidopsis AtMSRB5 functions as a salt-stress protector for both Arabidopsis and rice
Cai, Frontiers in plant science 2023 - “...for the other right-border (At4g04810 ( AtMSRB4 ) and At4g04800 ( AtMSRB3 )) and left-border (At4g04850) surrounding genes ( Supplementary Figure S2 ). Moreover, the transcripts of AtMSRB5 and AtMSRB6 were induced after 150 mM NaCl treatment in wild-type C24, although AtMSRB6 was gradually decreased after...”
- “...T-DNA insertion site. Quantitative analysis of At4g04810 ( AtMSRB4 ), At4g04800 ( AtMSRB3 ) and At4g04850 gene expression levels. Click here for additional data file. Supplementary Figure3 Transcription profiles of MSRB5 and MSRB6 in overexpressing lines of MSRB5 (B5OX), MSRB6 (B6OX) and MSRB5 plus MSRB6 (B5OX...”
- Chloroplast pH Homeostasis for the Regulation of Photosynthesis
Trinh, Frontiers in plant science 2022 - “...transporters and protein factors regulate thylakoid luminal pH. Locus Protein Subcellular localization Proposed function(s) References At4g04850 KEA3 Thylakoid membranes K + /H + antiporter NPQ control pH homeostasis Chanroj et al., 2012 Kunz et al., 2014 Armbruster et al., 2016 Wang et al., 2017 Wang and...”
- A metabolic, phylogenomic and environmental atlas of diatom plastid transporters from the model species Phaeodactylum
Liu, Frontiers in plant science 2022 - “...), such as probable homologues of the KEA K + /H + antiporters family (AT1G01790, AT4G04850 and AT1G01790). The P. tricornutum homologues of a thylakoid ADP/ATP carrier protein (AT5G01500), implicated in both ATP/ADP and 3-phosphoadenosine 5-phosphosulfate (PAPS) transport across the A. thaliana plastid envelope ( Thuswaldner...”
- Impaired photoprotection in Phaeodactylum tricornutum KEA3 mutants reveals the proton regulatory circuit of diatoms light acclimation
Seydoux, The New phytologist 2022 - “...). Bioinformatics The gene Phatr3_J39274 was identified as the most likely homologue of AtKEA3 (AtKEA3, At4g04850) by B last on the genome of P.tricornutum . Sequence alignment and phylogenetic reconstructions were performed using the function build of E te 3 v.3.1.1 (HuertaCepas etal ., 2016 )...”
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “...1193 128.034 5.22 0.08 Chloroplast 284350291203 AT4G00630 ATKEA2 4 1185 127.605 5.11 0.086 Chloroplast 261246268097 AT4G04850 ATKEA3 4 776 83.7907 5.53 0.369 Chloroplast 24526642457767 AT2G19600 ATKEA4 2 592 64.2494 5.91 0.589 Plasma membrane 84789708483854 AT5G51710 ATKEA5 5 568 61.5984 5.84 0.613 Plasma membrane 2100425121008849 AT5G11800 ATKEA6...”
- “...GmKEA1 AT4G00630 ATKEA2 LOC_Os12g42300.1 OsKEA2 GRMZM2G009715_P01 ZmKEA2 Potri.014G080800.1.p PtKEA2 Sobic.008G173800.1.p SbKEA2 Traes_2BL_BA71DD685.1 TaKEA2 Glyma.07G073700.1.p GmKEA2 AT4G04850 ATKEA3 LOC_Os06g36590.1 OsKEA3 GRMZM2G169114_P01 ZmKEA3 Potri.009G080800.2.p PtKEA3 Sobic.010G168900.1.p SbKEA3 Traes_2DL_053E73CFE.1 TaKEA3 Glyma.09G262000.1.p GmKEA3 AT2G19600 ATKEA4 LOC_Os03g03590.1 OsKEA4 GRMZM2G171031_P01 ZmKEA4 Potri.006G230400.1.p PtKEA4 Sobic.001G522100.1.p SbKEA4 Traes_5DS_49CF8A4C4.1 TaKEA4 Glyma.18G230100.1.p GmKEA4 AT5G51710 ATKEA5 GRMZM2G058948_P01...”
- More
SO1647 glutathione-regulated potassium-efflux system protein KefB, putative from Shewanella oneidensis MR-1
35% identity, 88% coverage
LOC123214717 K(+) efflux antiporter 3, chloroplastic from Mangifera indica
34% identity, 69% coverage
PSLF89_2203 cation:proton antiporter from Piscirickettsia salmonis LF-89 = ATCC VR-1361
33% identity, 87% coverage
KEFB_ALKAM / Q0ZAH7 Putative K(+) efflux antiporter KefB from Alkalimonas amylolytica (see paper)
TC 2.A.37.1.3 / Q0ZAH7 The K + efflux pump, KefB from Alkalimonas amylolytica (see paper)
34% identity, 86% coverage
- function: May operate as a K(+)/H(+) antiporter.
- substrates: K+
LHK_02018 KefB from Laribacter hongkongensis HLHK9
34% identity, 82% coverage
- Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
Lau, Cell & bioscience 2011 - “...(LHK_02296, LHK_00707) while the other two code for the putative genes kef (LHK_02848) and kefB (LHK_02018). No CDS encoding putative homologue of monovalent cation/proton antiporter-1 (CPA1) or monovalent cation/proton antiporter-3 (CPA3) has been identified. Transporters of the monovalent cation/proton antiporter (CPA) superfamily support key physiological functions...”
- “...Potassium uptake LHK_01488 - NMB0661 NGO0230 Glutathione-regulated potassium-efflux system protein kefB Potassium efflux, hydrogen influx LHK_02018 CV3326 NMB0209 - Glutathione-regulated potassium-efflux system protein kef Potassium efflux, hydrogen influx LHK_02848 - - NGO1774 Potassium-transporting ATPase, A subunit kdpA Potassium uptake LHK_01572 CV1599 - - Potassium-transporting ATPase, B...”
CV3326 glutathione-regulated potassium-efflux system protein from Chromobacterium violaceum ATCC 12472
36% identity, 81% coverage
- Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
Lau, Cell & bioscience 2011 - “...uptake LHK_01488 - NMB0661 NGO0230 Glutathione-regulated potassium-efflux system protein kefB Potassium efflux, hydrogen influx LHK_02018 CV3326 NMB0209 - Glutathione-regulated potassium-efflux system protein kef Potassium efflux, hydrogen influx LHK_02848 - - NGO1774 Potassium-transporting ATPase, A subunit kdpA Potassium uptake LHK_01572 CV1599 - - Potassium-transporting ATPase, B subunit...”
Bcep1808_2890 sodium/hydrogen exchanger from Burkholderia vietnamiensis G4
34% identity, 81% coverage
ZMO0846 sodium/hydrogen exchanger from Zymomonas mobilis subsp. mobilis ZM4
32% identity, 95% coverage
- Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates
Skerker, Molecular systems biology 2013 - “...a predicted efflux pump of unknown function ( ZMO1429-ZMO1432 ) and a sodium/hydrogen exchanger ( ZMO0846 ) ( Figure 5A ). ZMO0846 is a member of the KefB superfamily and may play a role in pH regulation. The presence of these outliers together with gloAB strongly...”
- “...Figure 5C and Supplementary Table 7 ). In Model-24, the biggest improved prediction was for ZMO0846 , which tends to be sensitive to organic acids at pH 6 but not in our unbuffered organic acid experiments ( Figure 3B ), consistent with its predicted role in...”
Hsero_3967 cation:proton antiporter from Herbaspirillum seropedicae SmR1
33% identity, 85% coverage
CJJ81176_1245 sodium/hydrogen exchanger family protein from Campylobacter jejuni subsp. jejuni 81-176
30% identity, 80% coverage
- The role of cydB gene in the biofilm formation by Campylobacter jejuni
Korkus, Scientific reports 2024 - “...transporter, periplasmic tungstate-binding protein, putative 15A3 CJJ81176_1185 Conserved hypothetical protein 15K CJJ81176_0128 Hypothetical protein C64 CJJ81176_1245 Sodium/hydrogen exchanger family protein E13 CJJ81176_0034 Hypothetical protein E17 CJJ81176_1363 Hypothetical protein E24 CJJ81176_1495 Bifunctional putA protein, putative E37 CJJ81176_0728 Conserved hypothetical protein TIGR00486 E40 CJJ81176_1309 Deoxycytidine triphosphate deaminase, putative...”
- Campylobacter jejuni Virulence Factors Identified by Modulating Their Synthesis on Ribosomes With Altered rRNA Methylation
Sałamaszyńska-Guz, Frontiers in cellular and infection microbiology 2021 - “...putative CJJ81176_1640 Flagellin CJJ81176_1338 Acetyltransferase, GNAT CJJ81176_0250 N-(5-phosphoribosyl)anthranilate isomerase, TrpF CJJ81176_0371 Sodium/hydrogen exchanger family protein CJJ81176_1245 Uncharacterized protein CJJ81176_1344 Preprotein translocase, YajC CJJ81176_1112 Para protein, Cjp26 CJJ81176_pVir0025 50S ribosomal protein L28 CJJ81176_0475 Tryptophan synthase alpha chain, TrpA CJJ81176_0373 Putative sugar transferase CJJ81176_1435 Carbon starvation protein A,...”
gll0383 probable potassium efflux transporter from Gloeobacter violaceus PCC 7421
33% identity, 74% coverage
- Function and evolution of channels and transporters in photosynthetic membranes
Pfeil, Cellular and molecular life sciences : CMLS 2014 - “...eukaryotes (a single gene lineage represented by the clades including Synechocystis ZP21042641, Synechocystis NhaS3, Gloeobacter gll0383, and two or more gene lineages for the clade including Gloeobacter KefC and Arabidopsis KEA1 to KEA3). Not all of these gene lineages appear to have remained in all descendents....”
CCO1319 glutathione-regulated potassium-efflux system protein from Campylobacter coli RM2228
32% identity, 79% coverage
Mhun_2803 sodium/hydrogen exchanger from Methanospirillum hungatei JF-1
32% identity, 82% coverage
- Complete genome sequence of Methanospirillum hungatei type strain JF1
Gunsalus, Standards in genomic sciences 2016 - “...predicted to be sodium dependent. Three genes encode Na +/ H + antiporters (Mhun_0680, Mhun_0841, Mhun_2803) that might maintain ion balance where the last differs by also possessing a Trk domain. Cell biosynthesis The genome of M. hungatei encodes an acetyl-CoA synthase/CO dehydrogenase complex (Cdh; Mhun_0686-0690)....”
Cla_0776 sodium/hydrogen exchanger family protein from Campylobacter lari RM2100
30% identity, 74% coverage
MM_1909 Glutathione-regulated potassium-efflux system protein from Methanosarcina mazei Goe1
28% identity, 77% coverage
PSLF89_2485 cation:proton antiporter from Piscirickettsia salmonis LF-89 = ATCC VR-1361
33% identity, 77% coverage
Q2JI45 Transporter, monovalent cation:proton antiporter-2 (CPA2) family from Synechococcus sp. (strain JA-2-3B'a(2-13))
32% identity, 86% coverage
DVU2302 glutathione-regulated potassium-efflux system protein KefB, putative from Desulfovibrio vulgaris Hildenborough
33% identity, 83% coverage
DET1627 sodium/hydrogen exchanger family protein from Dehalococcoides ethenogenes 195
31% identity, 91% coverage
alr2046 putative potassium/proton antiporter from Nostoc sp. PCC 7120
31% identity, 70% coverage
PBPRB0550 putative TrkA family protein from Photobacterium profundum SS9
38% identity, 56% coverage
- Laterally transferred elements and high pressure adaptation in Photobacterium profundum strains
Campanaro, BMC genomics 2005 - “...putative NAD(P)H oxidoreductase (PBPRB0548) and a putative TrkA family protein (glutathione-regulated potassium efflux system protein) (PBPRB0550). Furthermore PBPRB0559 gene has similarities with enterohemolysin 1, a gene also present in the Gifsy-1 prophage, and PBPRB0560 has similarities with exodeoxyribonuclease of the Gifsy-2 prophage of Salmonella typhimurium LT2...”
- “...such as NAD(P)H oxidoreductase (PBPRB0548), a putative TrkA family protein (glutathione-regulated potassium efflux system protein) (PBPRB0550) and tryptophanase (PBPRA1344) might be meaningful. The latter gene has two other paralogues in the SS9 genome one of which (PBPRA2532) is pressure regulated suggesting some role in deep-sea adaptation....”
Smlt3726 putative transmembrane transport protein from Stenotrophomonas maltophilia K279a
30% identity, 87% coverage
K0T731 Uncharacterized protein from Thalassiosira oceanica
36% identity, 40% coverage
DMR_28020 putative transport protein from Desulfovibrio magneticus RS-1
31% identity, 83% coverage
CIA_05132 YbaL family putative K(+) efflux transporter from Pseudomonas aeruginosa PA14
30% identity, 89% coverage
PA5518 probable potassium efflux transporter from Pseudomonas aeruginosa PAO1
29% identity, 89% coverage
DVDV_0709 monovalent cation:proton antiporter family protein from Desulfovibrio sp. DV
32% identity, 82% coverage
XF2140 cation:proton antiporter from Xylella fastidiosa 9a5c
30% identity, 87% coverage
WP_004387268 YbaL family putative K(+) efflux transporter from Cronobacter sakazakii
28% identity, 85% coverage
KPN_00459 putative transport protein from Klebsiella pneumoniae subsp. pneumoniae MGH 78578
28% identity, 85% coverage
- Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae
Hoppe, PloS one 2014 - “...listed in table 1 due to the attached HaloTag, are highlighted by red boxes. For KPN_00459, a putative transport protein, and KPN_00466, a hypothetical protein, bands of the correct size are hardly visible indicating a low expression, if at all. In contrast, the remaining samples revealed...”
- “...0.661.22 KPN_00363 [A6T5E4] Nucleoside channel; receptor of phage T6 and colicin K 294 33.5 0.770.14 KPN_00459 [A6T5N9] Putative transport protein 558 59.3 0.830.27 KPN_00466 [A6T5P6] Hypothetical protein 152 16.6 1.150.22 KPN_00786 [A6T6K1] Putative AcrB Cation/multidrug efflux pump 1021 111.5 0.470.13 KPN_01100 [A6T7G2] Histidine triad (HIT) protein...”
YlaA / b0478 putative transporter YbaL from Escherichia coli K-12 substr. MG1655 (see 5 papers)
YBAL_ECOLI / P39830 Putative cation/proton antiporter YbaL from Escherichia coli (strain K12) (see paper)
28% identity, 87% coverage
Z0597 putative transport protein from Escherichia coli O157:H7 EDL933
28% identity, 87% coverage
SF5M90T_448 YbaL family putative K(+) efflux transporter from Shigella flexneri 5a str. M90T
28% identity, 87% coverage
- RNA-seq analysis of the influence of anaerobiosis and FNR on Shigella flexneri
Vergara-Irigaray, BMC genomics 2014 - “...yjcE predicted cation/proton antiporter 1.49 SF5M90T_2675 cysN ATP-sulfurylase (ATP:sulfate adenylyltransferase), subunit 1 1.08 -2.08 -2.05 SF5M90T_448 ybaL putative transport protein -1.01 -0.50 SF5M90T_2386 mntH divalent metal cation transporter -1.93 1.61 1.68 SF5M90T_330 tauC taurine transport system permease protein -2.09 SF5M90T_3769 shiF putative membrane transport protein -2.16...”
Achr_31660 YbaL family putative K(+) efflux transporter from Azotobacter chroococcum NCIMB 8003
31% identity, 79% coverage
STM0492 putative CPA2 family transport protein from Salmonella typhimurium LT2
28% identity, 85% coverage
- Differential gene expression by RamA in ciprofloxacin-resistant Salmonella Typhimurium
Zheng, PloS one 2011 - “...STM0215 a methionine aminopeptidase 1.15E-13 map -2.3 STM0780 putative outer membrane or exported 3.61E-13 2.2 STM0492 putative CPA2 family transport protein 3.17E-13 ybaL -2.2 STM3311 putative ABC superfamily (bind_prot) transport protein 2.16E-15 yrbD 2.1 STM1004 nicotinate phosphoribosyltransferase 4.43E-11 pncB -2.1 STM1651 b putative pyruvate-flavodoxin oxidoreductase 5.61E-13...”
RC1_2885 sodium/hydrogen exchanger family protein from Rhodospirillum centenum SW
30% identity, 87% coverage
HP0471 glutathione-regulated potassium-efflux system protein (kefB) from Helicobacter pylori 26695
30% identity, 58% coverage
- <i>Helicobacter pylori</i> Efflux Pumps: A Double-Edged Sword in Antibiotic Resistance and Biofilm Formation
Krzyżek, International journal of molecular sciences 2024 - “...transporters, four of them showed a significant increase in the expression after exposure to clarithromycin: HP0471 (KefB, potassium ion transport), HP0497 (sodium and chlorine ion transport), HP0939 (YckJ, amino acid transport) and HP1017 (RocE, amino acid transport). It was proved that the average expression of genes...”
- “...with clarithromycin resistance than in susceptible ones. Additionally, it was observed that the deletion of hp0471 , hp0497 or hp0939 in clarithromycin-resistant strains was accompanied by a reduction or a complete loss of resistance to this antibiotic. In another original article by this team [ 64...”
- Transcending antibiotic resistance: The potential of mass Galla chinensis et camelliae Fermentata to Dismantle Helicobacter pylori biofilms and enhance anti-biotic activity
Zhang, Journal of ethnopharmacology 2024 (PubMed) (secret) - Clarithromycin sustained-release tablet may be an improper therapy for the eradication of Helicobacter pylori
Zuo, Therapeutic advances in gastroenterology 2024 - “...involved in H. pylori tolerance to clarithromycin by upregulating the transporters HP0939, HP1017, HP0497, and HP0471. For antibiotic-resistant strains, higher drug concentrations may result in superior bactericidal effects. This may explain why the sustained-release tablets were less effective than the dispersible tablets in this study. However,...”
- “...in the clarithromycin tolerance of Helicobacter pylori by upregulating the transporters HP0939, HP1017, HP0497, and HP0471 . Antimicrob Agents Chemother 2017 ; 61 ( 5 ): e02011e02016. 41 Chen CL Wu IT Wu DC , et al . Independent risk factors predicting eradication failure of hybrid...”
- Molecular Mechanisms of Biofilm Formation in <i>Helicobacter pylori</i>
Fauzia, Antibiotics (Basel, Switzerland) 2024 - “...forming was also reported [ 95 ]. Other efflux genes, hp0939 , hp0497 , and hp0471 , were highly expressed in the clinical isolates with multidrug resistance strains. When these genes were knocked out, the strains were more antibiotic-sensitive, and the biofilm formation was significantly reduced...”
- “...100 108 10.1016/j.micpath.2019.04.030 31034965 96. Cai Y. Wang C. Chen Z. Transporters HP0939, HP0497, and HP0471 participate in intrinsic multidrug resistance and biofilm formation in Helicobacter pylori by enhancing drug efflux Helicobacter 2020 25 e12715 10.1111/hel.12715 32548895 97. Grande R. Campli E.D. Bartolomeo S.D. Verginelli F....”
- Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice
Lin, Journal of clinical laboratory analysis 2023 - “...to the development of MDR in H.pylori strains. 125 In addition, transporters HP0939, HP0497, and HP0471 participate in intrinsic multidrug resistance in H.pylori by enhancing drug efflux, which significantly promotes the expression of hef A and glu P in MDR strains. 126 Moreover, the expression of...”
- “...respectively. 133 Interestingly, except for the above genes, the genes hp0939 , hp0497 , and hp0471 , which affect efflux pump function, were also found to be correlated with biofilm formation in H.pylori . 127 Experiments confirm that the corresponding knockout strains could hardly form biofilms,...”
- Biofilm of Helicobacter pylori: Life Cycle, Features, and Treatment Options
Elshenawi, Antibiotics (Basel, Switzerland) 2023 - “...hefD ), Hp1327 ( hefG ), Hp1489, Hp1118, Hp1174 ( gluP ), HP0939, HP0497, and HP0471 (KefB), were found to be expressed in both planktonic and biofilm cells, suggesting that efflux pump is essential during H. pylori life cycles [ 83 , 122 , 123 ]....”
- “...C. Chen Z. Xu Z. Li H. Li W. Sun Y. Transporters HP0939, HP0497, and HP0471 participate in intrinsic multidrug resistance and biofilm formation in Helicobacter pylori by enhancing drug efflux Helicobacter 2020 25 e12715 10.1111/hel.12715 32548895 124. Jolaiya T.F. Fowora M.A. Onyekwere C. Ugiagbe R....”
- Helicobacter pylori Biofilm-Related Drug Resistance and New Developments in Its Anti-Biofilm Agents
Hou, Infection and drug resistance 2022 - “...and the neutrophil-activating protein (NapA; HP0243). 28 , 29 The transporter proteins HP0939, HP0497, and HP0471 are implicated in H. pylori biofilm formation. 30 Methods for Detecting Biofilms The most frequently used staining method for the quantitative determination of in vitro biofilms grown attached in micro-titer...”
- “...Cai Y , Wang C , Chen Z , et al. Transporters HP0939, HP0497, and HP0471 participate in intrinsic multidrug resistance and biofilm formation in Helicobacter pylori by enhancing drug efflux . Helicobacter . 2020 ; 25 ( 4 ): e12715 . doi: 10.1111/hel.12715 32548895 31....”
- Genomic diversity of Helicobacter pylori populations from different regions of the human stomach
Wilkinson, Gut microbes 2022 - “...allelic diversity within the common allele dataset were those encoding the glutathione-regulated potassium-efflux system protein HP0471 (n=5), DNA-directed RNA polymerase subunit beta/beta (HP1198; n =5) and acetyl-CoA acetyltransferase (HP0690; n =5). There were 16,492 minor allelic variants within 1,738 genes from 33 deep-sequenced samples (Suppl. Table...”
- “...gene sabA (n=11), lipopolysaccharide biosynthesis genes (HP0208; HP0208; n =13) and the glutathione-regulated potassium-efflux system (HP0471; n =11) were also highly variable ( Figure 2 ; Suppl. Fig. 20). The length of H. pylori colonization time (years) was estimated for each patient and stomach location using...”
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HPF30_0851 cation:proton antiporter from Helicobacter pylori F30
30% identity, 58% coverage
- Microevolution of Virulence-Related Genes in Helicobacter pylori Familial Infection
Furuta, PloS one 2015 - “...subunit A HPF30_0629 M Putative outer membrane protein HPF30_0673 P kefB Glutathione-regulated potassium-efflux system protein HPF30_0851 O htrA Protease DO HPF30_0869 J infB Translation initiation factor IF-2 HPF30_0898 NT tlpA Methyl-accepting chemotaxis protein HPF30_1179 O clpA ATP-dependent C1p protease HPF30_1264 Response regulator HPF30_0871 (i) Family K-2:...”
Avin_10490 potassium proton antiporter from Azotobacter vinelandii AvOP
29% identity, 79% coverage
CV725_RS04480 cation:proton antiporter from Helicobacter pylori B128
30% identity, 58% coverage
- A Tripartite Efflux System Affects Flagellum Stability in Helicobacter pylori
Gibson, International journal of molecular sciences 2022 - “...cag pathogenicity island protein CV725_RS06350 2 bpAG coding (2657-2658/5466 nt) N886K 96.6% ESMV2 cation:proton antiporter CV725_RS04480 GCCACC A249T 98.9% fliL CV725_04935 (A)98 coding (46/552 nt) S16fs 97.3% murJ CV725_06605 (A)98 coding (1174/1462 nt) S392fs 98.6% hypothetical protein CV725_RS06835 CTACGA L4R 100% hypothetical protein CV725_RS06835 TTCTCC F5S...”
- “...coding (46/552 nt) S16fs 99.7% murJ CV725_06605 (A)98 coding (1174/1462 nt) S392fs 99.4% cation:proton antiporter CV725_RS04480 GCTACT A201T 99.1% a The numbers in parentheses indicates the position of the mutation (first number) within the entire length of the open reading frame (second number); b Indicates the...”
CLIBASIA_04115 potassium-efflux system protein from Candidatus Liberibacter asiaticus str. psy62
27% identity, 83% coverage
jhp0423 GLUTATHIONE-REGULATED POTASSIUM-EFFLUX SYSTEM PROTEIN from Helicobacter pylori J99
30% identity, 58% coverage
XOO1362 cation:proton antiporter from Xanthomonas oryzae pv. oryzae KACC10331
30% identity, 87% coverage
BAB1_1345 Binding-protein-dependent transport systems inner membrane component:ATP/GTP-binding site motif A (P-loop):TrkA-N:Potassium e... from Brucella melitensis biovar Abortus 2308
29% identity, 84% coverage
BH10840 Potassium-efflux system protein from Bartonella henselae str. Houston-1
26% identity, 85% coverage
XNC1_0944 YbaL family putative K(+) efflux transporter from Xenorhabdus nematophila ATCC 19061
27% identity, 85% coverage
BCAM1359 putative efflux pump/antiporter from Burkholderia cenocepacia J2315
29% identity, 83% coverage
- CytR Homolog of Pectobacterium carotovorum subsp. carotovorum Controls Air-Liquid Biofilm Formation by Regulating Multiple Genes Involved in Cellulose Production, c-di-GMP Signaling, Motility, and Type III Secretion System in Response to Nutritional and Environmental Signals
Haque, Frontiers in microbiology 2017 - “...Dickeya dadantii (formerly Erwinia chrysanthemi ) 3937 ( Haque et al., 2009 , 2012 ), Bcam1359 in Burkholderia cenocepacia H111 ( Fazli et al., 2011 ), QseC in Escherichia coli ( Hadjifrangiskou et al., 2012 ) and RscS in Vibrio fischeri ( Yip et al., 2006...”
LHK_02296 sodium/hydrogen exchanger from Laribacter hongkongensis HLHK9
29% identity, 83% coverage
- Environmental adaptability and stress tolerance of Laribacter hongkongensis: a genome-wide analysis
Lau, Cell & bioscience 2011 - “...antiporter-2 (CPA2) family. Two of the CDSs code for putative Na + /H + exchangers (LHK_02296, LHK_00707) while the other two code for the putative genes kef (LHK_02848) and kefB (LHK_02018). No CDS encoding putative homologue of monovalent cation/proton antiporter-1 (CPA1) or monovalent cation/proton antiporter-3 (CPA3)...”
- “...N. meningitidis MC58 N. gonorrhoeae FA 1090 Sodium/hydrogen exchanger - Sodium efflux, hydrogen influx LHK_00707 LHK_02296 CV2903 CV4147 - - Potassium uptake protein trkA Potassium uptake LHK_01490 - NMB1614 NGO1154 Potassium uptake protein trkH Potassium uptake LHK_01488 - NMB0661 NGO0230 Glutathione-regulated potassium-efflux system protein kefB Potassium...”
rosB / AAC60780.1 RosB from Yersinia enterocolitica (see 4 papers)
27% identity, 83% coverage
XP_006348050 K(+) efflux antiporter 5 isoform X1 from Solanum tuberosum
29% identity, 60% coverage
KEA5_ARATH / Q8VYR9 K(+) efflux antiporter 5; AtKEA5 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
TC 2.A.37.1.8 / Q8VYR9 K+ efflux antiporter 5 (AtKEA5) from Arabidopsis thaliana (see 3 papers)
AT5G51710 KEA5; potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
NP_568763 K+ efflux antiporter 5 from Arabidopsis thaliana
30% identity, 60% coverage
- function: Electroneutral K(+)/H(+) efflux antiporter involved in K(+) homeostasis and osmotic adjustment (PubMed:24278440, PubMed:30255504, PubMed:30309966, PubMed:31296940). Together with KEA4 and KEA6, promotes growth and development, and facilitates endosomal pH and ions homeostasis, as well as salt tolerance (e.g. K(+), NaCl and LiCl), probably by supporting cell wall biosynthesis during rapid etiolated seedling growth (PubMed:30255504, PubMed:30309966).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:30255504, PubMed:30309966). The triple mutant kea4 kea5 kea6 is compromised in cell wall biosynthesis and has a reduced growth, small rosettes and short seedlings, and is sensitive to low potassium K(+) availability and to high salinity (e.g. K(+), NaCl and LiCl); it also exhibits a reduced luminal pH in the Golgi, trans-Golgi network, prevacuolar compartment and vacuole (PubMed:30255504, PubMed:30309966). These phenotypes are partially suppressed by the cell wall-derived pectin homogalacturonan trigalacturonic GalA(3) in the dark but not in light conditions (PubMed:30255504). - substrates: K+
- SnRK1α1-mediated RBOH1 phosphorylation regulates reactive oxygen species to enhance tolerance to low nitrogen in tomato
Zheng, The Plant cell 2024 - “...AT4G33950 ARF6 Gramene: AT1G30330 ARF6 Araport: AT1G30330 HAK5 Gramene: AT4G13420 HAK5 Araport: AT4G13420 KEA5 Gramene: AT5G51710 KEA5 Araport: AT5G51710 NLP7 Gramene: AT4G24020 NLP7 Araport: AT4G24020 BIK1 Gramene: AT2G39660 BIK1 Araport: AT2G39660 ARF19 Gramene: AT1G19220 ARF19 Araport: AT1G19220 EDTA Gramene: Ethylene Diamine Tetraacetic Acid EDTA Araport: Ethylene...”
- Dynamic transcriptome analysis unravels key regulatory genes of maize root growth and development in response to potassium deficiency
Guo, Planta 2023 - “...al. 2013 ) Zm00001d018918 1.8 AT4G32500 AKT5 K+TRANSPORTER 5 (Pilot et al. 2003 ) Zm00001d031923 AT5G51710 KEA K+EFFLUX ANTIPORTER (Ahn et al. 2004 ) Zm00001d033067 2.6 2.9 4.0 AT4G13420 HAK5 HIGH AFFINITY K+TRANSPORTER 5 (Ragel et al. 2015 ) Zm00001d037289 4.1 AT5G37500 GORK GATED OUTWARDLY-RECTIFYING K+CHANNEL...”
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “...83.7907 5.53 0.369 Chloroplast 24526642457767 AT2G19600 ATKEA4 2 592 64.2494 5.91 0.589 Plasma membrane 84789708483854 AT5G51710 ATKEA5 5 568 61.5984 5.84 0.613 Plasma membrane 2100425121008849 AT5G11800 ATKEA6 5 597 64.3917 7.1 0.599 Plasma membrane 38033153808273 Ga08G1558.1 GaKEA1 8 1205 130.33 5.37 0.03 Chloroplast 104013215104021677() Ga12G1877.1 GaKEA2...”
- “...GmKEA3 AT2G19600 ATKEA4 LOC_Os03g03590.1 OsKEA4 GRMZM2G171031_P01 ZmKEA4 Potri.006G230400.1.p PtKEA4 Sobic.001G522100.1.p SbKEA4 Traes_5DS_49CF8A4C4.1 TaKEA4 Glyma.18G230100.1.p GmKEA4 AT5G51710 ATKEA5 GRMZM2G058948_P01 ZmKEA5 Potri.012G130500.1.p PtKEA5 Traes_5BS_6759CB1AD.1 TaKEA5 Glyma.08G064600.1.p GmKEA5 AT5G11800 ATKEA6 GRMZM2G040158_P01 ZmKEA6 Potri.018G054100.2.p PtKEA6 Traes_5AS_AF90452F5.1 TaKEA6 Glyma.07G184800.1.p GmKEA6 GRMZM2G474078_P01 ZmKEA7 Potri.015G132400.1.p PtKEA7 Traes_7DL_51216CD52.1 TaKEA7 Glyma.05G222500.1.p GmKEA7 Traes_7AL_23AA36A1B.1 TaKEA8 Glyma.08G029300.1.p...”
- Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis
Wang, Plant, cell & environment 2019 (PubMed) (secret) - Distinct leaf transcriptomic response of water deficient Eucalyptus grandis submitted to potassium and sodium fertilization
Favreau, PloS one 2019 - “...] Putative potassium/proton antiporter Eucgr.A02869 Potassium efflux antiporter (KEA5) -0.39** ns -0.39*** ns ns ns AT5G51710 [ 76 ] Eucgr.G01108 Putative potassium efflux antiporter (KEA3) ns ns -0.39* ns ns ns AT4G04850 [ 77 ] Non selective cation channel (NSCCs) Cyclic Nucleotide Gate Channels Eucgr.A01488 Putative...”
- K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments
Zhu, Plant physiology 2018 (PubMed)- “...article are as follows: KEA4 (At2g19600), KEA5 (At5g51710), KEA6 (At5g11800), NHX1 (At5g27150), NHX2 (At3g05030), NHX5 (At1g54370), and NHX6 (At1g79610)....”
- Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways
Pavlů, International journal of molecular sciences 2018 - “...[ 187 ] POT8 AT5G14880 Potassium transporter 8 1/2 [ 184 , 187 ] KEA5 AT5G51710 K + efflux antiporter 5 0/2 [ 183 ] KAT3 AT4G32650 Potassium channel KAT3 [ 184 ] SKOR AT3G02850 Potassium channel SKOR [ 184 , 188 , 189 ] AKT2...”
- Ectopic RING zinc finger gene from hot pepper induces totally different genes in lettuce and tobacco
Kesawat, Molecular breeding : new strategies in plant improvement 2018 - “...2 (TIP2) 0 0 0 2.86 At2g39890 Proline transporter 1 (PROT1) 0 0 0 3.27 At5g51710 K(+) efflux antiporter 5 (KEA5) 0 0 0 2.47 At1g79520 Cation efflux family protein 0 0 0 2.47 At3g55740 Proline transporter 2 (PROT2) 0 0 0 2.55 At1g80300 Nucleotide transporter...”
- More
- Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis.
Wang, Plant, cell & environment 2019 (PubMed)- GeneRIF: Golgi-localized and regulates ionic homeostasis and skotomorphogenesis
- K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments.
Zhu, Plant physiology 2018 - GeneRIF: KEA5 is important for growth.KEA5 is endosomal K(+) transporter functioning in maintaining pH and ion homeostasis in the endomembrane network.
B6SP24 Inner membrane protein ybaL from Zea mays
32% identity, 56% coverage
- Quantitative iTRAQ-based proteomic analysis of phosphoproteins and ABA-regulated phosphoproteins in maize leaves under osmotic stress
Hu, Scientific reports 2015 - “...difference occurred in vp5 ; the phosphorylation level changes of K + efflux antiporter 5-like (B6SP24) and sodium hydrogen exchanger 6-like (B4FS09) was only detected in vp5 . These results indicated that ABA might regulate the phosphorylation states of transporter proteins to maintain cell solute and...”
- “...family member 1-like (Q6UNK5), Na + /H + antiporter (B4FZY1), K + efflux antiporter 5-like (B6SP24) and sodium hydrogen exchanger 6-like (B4FS09) were regulated by ABA under osmotic stress. In summary, these results show that the phosphorylation and dephosphorylation of transporters might help the cell to...”
XP_049360980 K(+) efflux antiporter 4-like from Solanum verrucosum
32% identity, 62% coverage
PA5529 probable sodium/proton antiporter from Pseudomonas aeruginosa PAO1
30% identity, 65% coverage
KEA6_ARATH / B5X0N6 K(+) efflux antiporter 6; AtKEA6 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
NP_196741 K+ efflux antiporter 6 from Arabidopsis thaliana
AT5G11800 KEA6; potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
33% identity, 60% coverage
- function: Electroneutral K(+)/H(+) efflux antiporter involved in K(+) homeostasis and osmotic adjustment (PubMed:24278440, PubMed:30255504, PubMed:30309966, PubMed:31296940). Together with KEA4 and KEA5, promotes growth and development, and facilitates endosomal pH and ions homeostasis, as well as salt tolerance (e.g. K(+), NaCl and LiCl), probably by supporting cell wall biosynthesis during rapid etiolated seedling growth (PubMed:30255504, PubMed:30309966).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:30255504, PubMed:30309966). The triple mutant kea4 kea5 kea6 is compromised in cell wall biosynthesis and has small rosettes, short seedlings, and is sensitive to low potassium K(+) availability and to high salinity (e.g. K(+), NaCl and LiCl); it also exhibits a reduced luminal pH in the Golgi, trans-Golgi network, prevacuolar compartment and vacuole (PubMed:30255504, PubMed:30309966). These phenotypes are partially suppressed by the cell wall-derived pectin homogalacturonan trigalacturonic GalA(3) in the dark but not in light conditions (PubMed:30255504). - Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis.
Wang, Plant, cell & environment 2019 (PubMed)- GeneRIF: Golgi-localized and regulates ionic homeostasis and skotomorphogenesis
- K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments.
Zhu, Plant physiology 2018 - GeneRIF: KEA6 is important for growth.KEA6 is endosomal K(+) transporter functioning in maintaining pH and ion homeostasis in the endomembrane network.
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “...5.91 0.589 Plasma membrane 84789708483854 AT5G51710 ATKEA5 5 568 61.5984 5.84 0.613 Plasma membrane 2100425121008849 AT5G11800 ATKEA6 5 597 64.3917 7.1 0.599 Plasma membrane 38033153808273 Ga08G1558.1 GaKEA1 8 1205 130.33 5.37 0.03 Chloroplast 104013215104021677() Ga12G1877.1 GaKEA2 12 1209 129.982 5.23 0.12 Chloroplast 30757828-30767684(+) Ga03G2727.1 GaKEA3 3...”
- “...SbKEA4 Traes_5DS_49CF8A4C4.1 TaKEA4 Glyma.18G230100.1.p GmKEA4 AT5G51710 ATKEA5 GRMZM2G058948_P01 ZmKEA5 Potri.012G130500.1.p PtKEA5 Traes_5BS_6759CB1AD.1 TaKEA5 Glyma.08G064600.1.p GmKEA5 AT5G11800 ATKEA6 GRMZM2G040158_P01 ZmKEA6 Potri.018G054100.2.p PtKEA6 Traes_5AS_AF90452F5.1 TaKEA6 Glyma.07G184800.1.p GmKEA6 GRMZM2G474078_P01 ZmKEA7 Potri.015G132400.1.p PtKEA7 Traes_7DL_51216CD52.1 TaKEA7 Glyma.05G222500.1.p GmKEA7 Traes_7AL_23AA36A1B.1 TaKEA8 Glyma.08G029300.1.p GmKEA8 Traes_4BL_723A7E539.1 TaKEA9 Glyma.17G229700.1.p GmKEA9 Traes_7BL_B01B8C760.1 TaKEA10 Glyma.14G093900.1.p GmKEA10 Traes_5AL_91132361F.1...”
- Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis
Wang, Plant, cell & environment 2019 (PubMed) (secret) - K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments
Zhu, Plant physiology 2018 (PubMed)- “...as follows: KEA4 (At2g19600), KEA5 (At5g51710), KEA6 (At5g11800), NHX1 (At5g27150), NHX2 (At3g05030), NHX5 (At1g54370), and NHX6 (At1g79610). Supplemental Data...”
- The temporal foliar transcriptome of the perennial C3 desert plant Rhazya stricta in its natural environment
Yates, BMC plant biology 2014 - “...to midday a number of homologs to Arabidopsis genes encoding transporter proteins were found (AT5G12080, AT5G11800, AT5G57490, AT4G05120, AT5G15640). In the high light+heat, midday to dusk, four homologs to xyloglucan endotransglucosylase/hydrolases were found, which are involved in cell wall modification. Table 2 Comparison of R. stricta...”
MAGA_PARM1 / Q2W031 Iron transporter MagA from Paramagnetospirillum magneticum (strain ATCC 700264 / AMB-1) (Magnetospirillum magneticum) (see 2 papers)
amb3990 ferrous transporter from Magnetospirillum magneticum AMB-1
31% identity, 58% coverage
XP_049403596 K(+) efflux antiporter 6-like from Solanum stenotomum
31% identity, 56% coverage
FTL_0943 Sodium/hydrogen exchanger family protein from Francisella tularensis subsp. holarctica
29% identity, 62% coverage
GERN_BACCE / Q9KI10 Na(+)/H(+)-K(+) antiporter GerN from Bacillus cereus (see 3 papers)
TC 2.A.37.2.2 / Q9KI10 The Na+/H+-K+ antiporter, GerN (spore germination protein-N) from Bacillus cereus (see paper)
30% identity, 57% coverage
- function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Can also use potassium as a coupling ion, without completely replacing H(+). This Na(+)/H(+)-K(+) antiport is much more rapid than Na(+)/H(+) antiport. Can also extrude lithium. Important for the inosine-dependent germination of spores.
disruption phenotype: Disruption causes a major defect in inosine- dependent germination, but does not significantly affect germination in response to L-alanine. - substrates: H+, K+, Na+
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...of the CPA2 domain of AtKEA3, EcNhaA, EcKefB (P45522) EcKefC, TtNapA, Bacillus cereus GerN (BcGerN, Q9KI10), Synechocystis NhaS3 (Q55190) and NhaS4 (Q5N3F5), Arabidopsis AtCHX17 (Q9SUQ7), AtKEA2 and Saccharomyces cerevisiae KHA1 (P40309) were performed using ClustalOmega. KEA3 co-expression analysis was performed by determining Pearson correlation coefficients of...”
LOC123200724 K(+) efflux antiporter 6 from Mangifera indica
32% identity, 56% coverage
LOC103949642 K(+) efflux antiporter 6 from Pyrus x bretschneideri
34% identity, 56% coverage
LOC123226529 K(+) efflux antiporter 5-like from Mangifera indica
30% identity, 60% coverage
KEA4_ARATH / Q9ZUN3 K(+) efflux antiporter 4; AtKEA4 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
NP_849990 K+ efflux antiporter 4 from Arabidopsis thaliana
AT2G19600 ATKEA4; potassium ion transmembrane transporter/ potassium:hydrogen antiporter from Arabidopsis thaliana
32% identity, 56% coverage
- function: Electroneutral K(+)/H(+) efflux antiporter involved in K(+) homeostasis and osmotic adjustment (PubMed:24278440, PubMed:30255504, PubMed:30309966, PubMed:31296940). Together with KEA5 and KEA6, promotes growth and development, and facilitates endosomal pH and ions homeostasis, as well as salt tolerance (e.g. K(+), NaCl and LiCl), probably by supporting cell wall biosynthesis during rapid etiolated seedling growth (PubMed:30255504, PubMed:30309966).
catalytic activity: K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in) (RHEA:29467)
disruption phenotype: No visible phenotype (PubMed:30255504, PubMed:30309966). The triple mutant kea4 kea5 kea6 is compromised in cell wall biosynthesis and has small rosettes, short seedlings, and is sensitive to low potassium K(+) availability and to high salinity (e.g. K(+), NaCl and LiCl); it also exhibits a reduced luminal pH in the Golgi, trans-Golgi network, prevacuolar compartment and vacuole (PubMed:30255504, PubMed:30309966). These phenotypes are partially suppressed by the cell wall-derived pectin homogalacturonan trigalacturonic GalA(3) in the dark but not in light conditions (PubMed:30255504). - Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis.
Wang, Plant, cell & environment 2019 (PubMed)- GeneRIF: Golgi-localized and regulates ionic homeostasis and skotomorphogenesis
- K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments.
Zhu, Plant physiology 2018 - GeneRIF: KEA4 is important for growth.KEA4 is endosomal K(+) transporter functioning in maintaining pH and ion homeostasis in the endomembrane network.
- Silencing of GhKEA4 and GhKEA12 Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton
Li, Frontiers in plant science 2021 - “...1185 127.605 5.11 0.086 Chloroplast 261246268097 AT4G04850 ATKEA3 4 776 83.7907 5.53 0.369 Chloroplast 24526642457767 AT2G19600 ATKEA4 2 592 64.2494 5.91 0.589 Plasma membrane 84789708483854 AT5G51710 ATKEA5 5 568 61.5984 5.84 0.613 Plasma membrane 2100425121008849 AT5G11800 ATKEA6 5 597 64.3917 7.1 0.599 Plasma membrane 38033153808273 Ga08G1558.1...”
- “...GmKEA2 AT4G04850 ATKEA3 LOC_Os06g36590.1 OsKEA3 GRMZM2G169114_P01 ZmKEA3 Potri.009G080800.2.p PtKEA3 Sobic.010G168900.1.p SbKEA3 Traes_2DL_053E73CFE.1 TaKEA3 Glyma.09G262000.1.p GmKEA3 AT2G19600 ATKEA4 LOC_Os03g03590.1 OsKEA4 GRMZM2G171031_P01 ZmKEA4 Potri.006G230400.1.p PtKEA4 Sobic.001G522100.1.p SbKEA4 Traes_5DS_49CF8A4C4.1 TaKEA4 Glyma.18G230100.1.p GmKEA4 AT5G51710 ATKEA5 GRMZM2G058948_P01 ZmKEA5 Potri.012G130500.1.p PtKEA5 Traes_5BS_6759CB1AD.1 TaKEA5 Glyma.08G064600.1.p GmKEA5 AT5G11800 ATKEA6 GRMZM2G040158_P01 ZmKEA6 Potri.018G054100.2.p PtKEA6 Traes_5AS_AF90452F5.1...”
- Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis
Wang, Plant, cell & environment 2019 (PubMed) (secret) - K+ Efflux Antiporters 4, 5, and 6 Mediate pH and K+ Homeostasis in Endomembrane Compartments
Zhu, Plant physiology 2018 (PubMed)- “...in this article are as follows: KEA4 (At2g19600), KEA5 (At5g51710), KEA6 (At5g11800), NHX1 (At5g27150), NHX2 (At3g05030), NHX5 (At1g54370), and NHX6...”
BC1612 Na+/H+ antiporter NapA (inosine-dependent germination) from Bacillus cereus ATCC 14579
29% identity, 57% coverage
XP_006360984 K(+) efflux antiporter 4 from Solanum tuberosum
32% identity, 56% coverage
LOC106771900 K(+) efflux antiporter 5 from Vigna radiata var. radiata
29% identity, 56% coverage
BA1639 germination protein gerN from Bacillus anthracis str. Ames
GBAA1639 germination protein gerN from Bacillus anthracis str. 'Ames Ancestor'
29% identity, 57% coverage
3l9wB / P03819,P0A754 Kefc c-terminal domain in complex with keff and gsh (see paper)
42% identity, 27% coverage
- Ligands: flavin mononucleotide; adenosine monophosphate; glutathione (3l9wB)
3l9xA / P03819,P0A754 Kefc c-terminal domain in complex with keff and esg (see paper)
45% identity, 24% coverage
- Ligands: flavin mononucleotide; zinc ion; l-gamma-glutamyl-s-[(3s)-1-ethyl-2,5-dioxopyrrolidin-3-yl]-l-cysteinylglycine; l-gamma-glutamyl-s-[(3r)-1-ethyl-2,5-dioxopyrrolidin-3-yl]-l-cysteinylglycine (3l9xA)
GERT_BACCE / B3VQ24 Probable Na(+)/H(+) antiporter GerT from Bacillus cereus (see paper)
26% identity, 57% coverage
- function: Contributes to the success of spore outgrowth from the germinated state during alkaline or Na(+) stress. Does not have a significant role in germination.
BA0819 germination protein gerN from Bacillus anthracis str. Ames
26% identity, 57% coverage
CDL62_00920 cation:proton antiporter from Alkalitalea saponilacus
24% identity, 79% coverage
lp_2827 Na(+)/H(+) antiporter from Lactobacillus plantarum WCFS1
26% identity, 58% coverage
LP125_003204 cation:proton antiporter from Lactiplantibacillus plantarum
26% identity, 58% coverage
IGB08_11065 cation:proton antiporter from Lactiplantibacillus plantarum
26% identity, 58% coverage
LSA_RS01505 cation:proton antiporter from Fructilactobacillus sanfranciscensis TMW 1.1304
26% identity, 61% coverage
VF_0805 glutathione-regulated potassium-efflux system protein KefC from Vibrio fischeri ES114
VF_0805 cation:proton antiporter family protein from Aliivibrio fischeri ES114
24% identity, 90% coverage
YP_144738 Na(+)/H(+) antiporter from Thermus thermophilus HB8
Q72IM4 Na(+)/H(+) antiporter from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
28% identity, 61% coverage
- Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane Na+/H+ Antiporter of Schizosaccharomyces pombe
Dutta, Scientific reports 2019 - “...) and Rattus norvegicus NHE1 ( AAA98479 ) are included. Finally, Thermus thermophiles NapA ( YP_144738 ), and Escherichia coli NhaA( WP_000681354 ) are from the bacterial group. Putative TM segments TM3, TM10, and parts of TM12-TM13 of Sp Nhe1 are indicated on top of the...”
- Transmembrane Segment XI of the Na+/H+ Antiporter of S. pombe is a Critical Part of the Ion Translocation Pore
Dutta, Scientific reports 2017 - “...jannaschii NhaP1 ( NP_247021.1 ), Pyrococcus Abyssii NhaP ( CAB50204 ), Thermus thermophiles NapA ( YP_144738 ), and Escherichia coli NhaA ( WP_000681354 ) are included as acheal and bacterial members. From plant groups Arabidopsis thaliana SOS1 ( AAL32824 ), Brassica napus SOS1 ( AGA37213.1 ),...”
- “...jannaschii NhaP1 ( NP_247021.1 ), Pyrococcus Abyssii NhaP ( CAB50204 ), Thermus thermophiles NapA ( YP_144738 ), and Escherichia coli NhaA ( WP_000681354 ) are included as acheal and bacterial members. Plant Arabidopsis thaliana SOS1 ( AAL32824 ), Brassica napus SOS1 ( AGA37213.1 ), and Theobroma...”
- A two-domain elevator mechanism for sodium/proton antiport
Lee, Nature 2013 - “...relevant to all types of ion-coupled transporters. METHODS Thermus thermophilus NapA sequence (uniprot accession number: Q72IM4); residues progressively substituted to cysteine are underlined and in bold and additional C-terminal residues retained after TEV cleavage are shown in italics (see next section for cloning details). MHGAEHLLEIFYLLLAAQV M...”
PWH42_09585 cation:proton antiporter from Pediococcus acidilactici
27% identity, 57% coverage
- Draft Genome Sequence of a Putative Raw Milk-Associated Probiotic Bacterium, Pediococcus acidilactici ISO17
Makete, Microbiology resource announcements 2023 - “...MDD9322703.1 Na+/H+ antiporter NhaC family protein PWH42_03175 MDD9323008.1 Na+/H+ antiporter NhaC PWH42_05505 MDD9323464.1 Sodium/proton antiporter PWH42_09585 MDD9324247.1 Cation/proton antiporter Antioxidant PWH42_00180 MDD9322436.1 Thioredoxin PWH42_00350 MDD9322470.1 Thiol peroxidase PWH42_00985 MDD9322593.1 Thioredoxin-disulfide reductase PWH42_01605 MDD9322699.1 Catalase PWH42_02920 MDD9322957.1 Peptide-methionine (S)- S -oxide reductase MsrA PWH42_02925 MDD9322958.1 Peptide-methionine (R)-...”
blr2614 blr2614 from Bradyrhizobium japonicum USDA 110
26% identity, 81% coverage
VV1009 putative glutathione-regulated potassium-efflux system protein KefB from Vibrio vulnificus YJ016
26% identity, 84% coverage
- Genotype is correlated with but does not predict virulence of Vibrio vulnificus biotype 1 in subcutaneously inoculated, iron dextran-treated mice
Thiaville, Infection and immunity 2011 - “...60) and ATCC 23907 (25); MLT365, MLT367, MLT403, VV1009, 2400112, and LL728, previously examined by our group for virulence (51, 52); and genomically sequenced...”
- “...MLT403 S1-10 S1-13 S1-8 S2-10 S2-20 S2-22 S3-16 S3-5 VV1009 YJ016 1197 PCR using primers of Chatzidaki-Livanis et al. (12). The 16S rRNA (rrn) type was...”
- Real-time PCR analysis of Vibrio vulnificus from oysters
Campbell, Applied and environmental microbiology 2003 - “...6353/O 1015H EDL174/O LC4/O LC4/T 5C1326/O 85A667/O 2400112 LL728 VV1009 345/O 345/T UNCC 913 UNCC1015 MLT 365 MLT 367 MLT 403 409/O 80363/O Clinical, Calif....”
- Phage therapy of local and systemic disease caused by Vibrio vulnificus in iron-dextran-treated mice
Cerveny, Infection and immunity 2002 - “...used four clinical isolates of V. vulnificus, M06-24/0, VV1009, 2400112, and NSV-5829, obtained from patients who died from sepsis following ingestion of...”
- “...ml each of LB-N cultures of V. vulnificus strains VV1009, LL728, 2400112, MLT403, MLT365, and MLT367. The mixture was shaken overnight at 30C. On the next day,...”
- Recovery of hydrogen peroxide-sensitive culturable cells of Vibrio vulnificus gives the appearance of resuscitation from a viable but nonculturable state
Bogosian, Journal of bacteriology 2000 - “...of sepsis following ingestion of contaminated oysters, designated VV1009 and 2400-112 (31a, 32); these strains were obtained from P. A. Gulig, University of...”
- “...the plate counts. For the strains MLT367, MLT403, VV1009, and 2400-112, the maximum plate counts observed were about 1.5- to 3-fold Downloaded from...”
- Pathogenesis of infection by clinical and environmental strains of Vibrio vulnificus in iron-dextran-treated mice
Starks, Infection and immunity 2000 - “...clinical isolates of V. vulnificus (LL728, 2400112, and VV1009) from patients who died of sepsis following ingestion of contaminated oysters and three...”
- “...experimental treatments. ter-associated sepsis (LL728, 2400112, and VV1009) and the three environmental isolates (MLT365, MLT367, and MLT 403) all demonstrated...”
5nc8A / Q12SB3 Shewanella denitrificans kef ctd in amp bound form (see paper)
39% identity, 25% coverage
- Ligand: adenosine monophosphate (5nc8A)
Synpcc7942_0546 Na+/H+ antiporter from Synechococcus elongatus PCC 7942
29% identity, 52% coverage
- The Freshwater Cyanobacterium Synechococcus elongatus PCC 7942 Does Not Require an Active External Carbonic Anhydrase
Kupriyanova, Plants (Basel, Switzerland) 2024 - “...Synpcc7942_1468, Synpcc7942_1469, Synpcc7942_1473, and Synpcc7942_1474); potential Na + /H + antiporters Nha17 (Synpcc7942_0811, Synpcc7942_1264, Synpcc7942_2359, Synpcc7942_0546, Synpcc7942_0307, Synpcc7942_2394, and Synpcc7942_2186); as well as the PxcA proton pump (Synpcc7942_0991). The following genes were selected for this study: (1) ndhD5 (Synpcc7942_1473), which encodes one of the Mnh complexs...”
4bwzA / Q72IM4 Crystal structure of the sodium proton antiporter, napa (see paper)
28% identity, 59% coverage
LSA1703 Putative Na(+)/H(+) antiporter from Lactobacillus sakei subsp. sakei 23K
25% identity, 62% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...-1.0 -0.8 LSA1645 lsa1645 Putative Na(+)/(+) antiporter 1.4 D LSA1699 mntH2 Mn(2+)/Fe(2+) transport protein -0.6 LSA1703 lsa1703 Putative Na(+)/H(+) antiporter -1.2 LSA1704 lsa1704 Putative calcium-transporting P-type ATPase -0.8 LSA1735 lsa1735 Putative cobalt ABC transporter, membrane-spanning subunit -0.6 LSA1736 lsa1736 Putative cobalt ABC transporter, ATP-binding subunit -0.6...”
CHX17_ARATH / Q9SUQ7 Cation/H(+) antiporter 17; Protein CATION/H+ EXCHANGER 17; AtCHX17 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
TC 2.A.37.4.2 / Q9SUQ7 Endosome and prevacuole K+:H+ antiporter, CHX17 of 820 aas and 13 putative TMSs from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
NP_194101 cation/H+ exchanger 17 from Arabidopsis thaliana
AT4G23700 ATCHX17 (CATION/H+ EXCHANGER 17); monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
26% identity, 43% coverage
- function: Operates as a K(+)/H(+) antiporter that controls K(+) acquisition and homeostasis.
disruption phenotype: Decreased K(+) content in roots. - substrates: H+, K+
tcdb comment: Mediates potassium ion and pH homeomeostasis, thereby influencing membrane trafficking (Chanroj et al. 2011). May also catalyze Na+/H+ antiport (Sze and Chanroj 2018) - Transporters involved in pH and K+ homeostasis affect pollen wall formation, male fertility, and embryo development.
Padmanaban, Journal of experimental botany 2017 - GeneRIF: Fertility could be partially compromised by impaired pollen tube and/or sperm function as CHX19 and CHX18 are expressed in the pollen tube and sperm cell, respectively. CHX17 and CHX18 proteins may promote embryo development possibly through the endosperm where these genes are expressed.
- Protein architecture and core residues in unwound α-helices provide insights to the transport function of plant AtCHX17.
Czerny, Biochimica et biophysica acta 2016 (PubMed)- GeneRIF: The core of AtCHX17 models according to EcNhaA and TtNapA templates faces inward and outward, respectively, which may reflect two conformational states of the alternating access transport mode for proteins belonging to the plant CHX family
- K+ transporter AtCHX17 with its hydrophilic C tail localizes to membranes of the secretory/endocytic system: role in reproduction and seed set.
Chanroj, Molecular plant 2013 (PubMed)- GeneRIF: successful reproduction and seed development depend on the ability to regulate cation and pH homeostasis by AtCHX17-like transporters on membranes that traffic in the endocytic and/or secretory pathways
- Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting.
Chanroj, The Journal of biological chemistry 2011 - GeneRIF: Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting.
- Two members of a network of putative Na+/H+ antiporters are involved in salt and pH tolerance of the freshwater cyanobacterium Synechococcus elongatus
Billini, Journal of bacteriology 2008 - “...NP_391222), and ATCHX17 (A. thaliana) (accession number NP_194101). Downloaded from http://jb.asm.org/ on February 13, 2017 by University of California,...”
- Arabidopsis thaliana CHX17 gene complements the kha1 deletion phenotypes in Saccharomyces cerevisiae.
Maresova, Yeast (Chichester, England) 2006 (PubMed)- GeneRIF: results show that the proteins Arabidopsis thaliana Chx17 and Saccharomyces cerevisiae Kha1 could have similar functions.
- Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis.
Cellier, The Plant journal : for cell and molecular biology 2004 (PubMed)- GeneRIF: AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones.
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...EcKefC, TtNapA, Bacillus cereus GerN (BcGerN, Q9KI10), Synechocystis NhaS3 (Q55190) and NhaS4 (Q5N3F5), Arabidopsis AtCHX17 (Q9SUQ7), AtKEA2 and Saccharomyces cerevisiae KHA1 (P40309) were performed using ClustalOmega. KEA3 co-expression analysis was performed by determining Pearson correlation coefficients of all Arabidopsis genes with KEA3 using the GeneCAT database...”
- Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana
Liaqat, BMC plant biology 2023 - “...), AtRGL3 ( At5G17490 ), AtEDL3 ( At3G63060 ), AtRAS1 ( At1G09950 ), AtCHX17 ( At4G23700 ), AtHAK5 ( At4G13420 ), AtCAX1 ( At2G38170 ), AtCAX3 ( At3G51860 ). Supplementary Information Additional file 1: Fig. S1. Confirmation of CRISPR/cas9-edited AtSPT4-2 knockout mutants through sequencing and expression...”
- NHX Gene Family in Camellia sinensis: In-silico Genome-Wide Identification, Expression Profiles, and Regulatory Network Analysis
Paul, Frontiers in plant science 2021 - “...for K + nutrition, K + /Na + selectivity, and salt tolerance. CHX18 (AT5G41610), CHX17 (AT4G23700), and CHX15 (AT2G13620) are all involved in regulation of pH and are members of the putative Na + /H + antiporter family ( Berardini et al., 2015 ). Figure 7...”
- Dynamic Development of White Lupin Rootlets Along a Cluster Root
Le, Frontiers in plant science 2021 - “...Potassium Profile 6 Lalb_Chr24g0393761 AT4G10310 High-affinity K + transporter 1 (HKT1) Potassium Profile 5 Lalb_Chr09g0332731 AT4G23700 Cation/H + exchanger 17 (CHX17) Sodium Profile 5 Lalb_Chr05g0210921 AT5G26340 Sugar transport protein 13 (STP13) Sugar Profile 6 Lalb_Chr06g0160591 AT5G26340 Sugar transport protein 13 (STP13) Sugar Profile 5 Lalb_Chr20g0123111 AT1G22150...”
- Surface pH changes suggest a role for H+/OH- channels in salinity response of Chara australis
Absolonova, Protoplasma 2018 - “...in C. australis was detected, which shares 40% amino acid identities with A. thaliana CHX17 (At4g23700) (Suppl. Fig. 5 ). Furthermore, we were able to find several partial protein sequences that showed 3040% amino acid identity to various voltage-gated K + channels of A. thaliana ....”
- “...exchanger superfamily in Chara , which shares 40% amino acid identities with A. thaliana CHX17 (At4g23700) (Suppl. Fig. 5 ). AtCHX17 is involved in regulation of pH homeostasis and is associated with the plasma membrane and post-Golgi compartments (Cellier et al. 2004 ; Chanroja et al....”
- Transcriptome profiling of genes involved in induced systemic salt tolerance conferred by Bacillus amyloliquefaciens FZB42 in Arabidopsis thaliana
Liu, Scientific reports 2017 - “...x-axis indicates the 35 selected genes (from left to right: AT5G44420, AT3G23250, AT4g26200, AT1g17420, AT4G39210, AT4G23700, AT1g29910, AT2G05070, ATCG00120, ATCG00580, AT2G39800, AT2G05100, AT2G34420, AT3G27690, AT5G54270, AT4G39770, AT1G64170, AT1G14540, AT1G70290, AT3g14440, AT4G13250, AT1G78090, AT4G36110, AT5G23370, AT3G24500, AT3G03480, AT4G08390, AT1G05560, AT5G51720, AT4G32810, AT3G12580, AT3G04800, AT1G64900, AT3G44990, and AT1G10070)....”
- Transporters involved in pH and K+ homeostasis affect pollen wall formation, male fertility, and embryo development
Padmanaban, Journal of experimental botany 2017 - “...ACT11:MSI1:GFP signal as described before ( Leydon et al. , 2015 ). Accession numbers CHX17 (At4g23700), CHX18 (At5g41610), and CHX19 (At3g17630) Results chx17chx18chx19 mutants show reduced seed set Vegetative growth and flower development of chx17chx18chx19 plants were similar to those of the wild type ( Fig....”
- Transcriptomic Analysis of Soil-Grown Arabidopsis thaliana Roots and Shoots in Response to a Drought Stress
Rasheed, Frontiers in plant science 2016 - “...75.1 4.6 4.7 17.8 24.3 At5g52300 RD29B 8.4 68.4 164.7 335.9 5.7 7.2 76.6 116.6 At4g23700 Cation/H + exchanger 17 4.3 3.0 5.1 5.0 0.5 0.4 0.3 1.7 At3g13784 FRUCT6/CWINV5 5.5 7.4 5.4 9.9 1.5 1.5 11.7 48.4 At2g43570 Chitinase, putative 4.6 1.5 1.9 2.6 1.2...”
- Transcriptomic profiling of linolenic acid-responsive genes in ROS signaling from RNA-seq data in Arabidopsis
Mata-Pérez, Frontiers in plant science 2015 - “...Arabidopsis thaliana high affinity nitrate transporter 2.6; XTH31 (AT3G44990), Arabidopsis thaliana xyloglucan endotransglycosylase/hydrolase 31; CHX17 (AT4G23700), Arabidopsis thaliana cation/H(+) antiporter 17; PTR3 (AT5G46050), Arabidopsis thaliana putative peptide transporter protein 3; CLV3 (AT2G27250), Arabidopsis thaliana protein CLAVATA 3; OZF1 (AT2G19810), Arabidopsis thaliana Oxidation-related Zinc Finger protein 1;...”
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lp_3565 Na(+)/H(+) antiporter from Lactobacillus plantarum WCFS1
24% identity, 57% coverage
YP_001475696 sodium/hydrogen exchanger from Shewanella sediminis HAW-EB3
26% identity, 58% coverage
IGB08_06005 cation:proton antiporter from Lactiplantibacillus plantarum
25% identity, 58% coverage
XP_006366744 cation/H(+) antiporter 15-like from Solanum tuberosum
28% identity, 45% coverage
NSCAC_1736 cation:proton antiporter from Candidatus Nitrosacidococcus tergens
27% identity, 79% coverage
VS_2263 putative glutathione-regulated potassium-efflux system protein KefB from Vibrio splendidus LGP32
28% identity, 68% coverage
NAPA_ENTHR / P26235 Na(+)/H(+) antiporter from Enterococcus hirae (see 2 papers)
TC 2.A.37.2.1 / P26235 Na+:H+ antiporter, NapA from Enterococcus hirae (see 2 papers)
27% identity, 59% coverage
- function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Can also transport lithium.
- substrates: H+, Na+
lpg1507 sodium/hydrogen antiporter from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
27% identity, 62% coverage
TMCO3_HUMAN / Q6UWJ1 Transmembrane and coiled-coil domain-containing protein 3; Putative LAG1-interacting protein from Homo sapiens (Human) (see paper)
NP_060375 transmembrane and coiled-coil domain-containing protein 3 isoform 1 precursor from Homo sapiens
28% identity, 52% coverage
- function: Probable Na(+)/H(+) antiporter.
- M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer.
Ran, Communications biology 2024 - GeneRIF: M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer.
- TMCO3, a Putative K+ :Proton Antiporter at the Golgi Apparatus, Is Important for Longitudinal Growth in Mice and Humans.
Holling, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2023 (PubMed)- GeneRIF: TMCO3, a Putative K[+] :Proton Antiporter at the Golgi Apparatus, Is Important for Longitudinal Growth in Mice and Humans.
- Mutations in the TMCO3 Gene are Associated with Cornea Guttata and Anterior Polar Cataract.
Chen, Scientific reports 2016 - GeneRIF: This study reveals, for the first time, that mutations in TMCO3 are associated with cornea guttata and anterior polar cataract, warranting further investigation into the pathogenesis of this disorder.
- A Novel GLP1 Receptor Interacting Protein ATP6ap2 Regulates Insulin Secretion in Pancreatic Beta Cells.
Dai, The Journal of biological chemistry 2015
XP_006360740 cation/H(+) antiporter 18-like from Solanum tuberosum
26% identity, 45% coverage
all1303 Na+/H+ antiporter from Nostoc sp. PCC 7120
26% identity, 62% coverage
VC0992 glutathione-regulated potassium-efflux system protein KefB, putative from Vibrio cholerae O1 biovar eltor str. N16961
25% identity, 83% coverage
LSA1645 Putative Na(+)/(+) antiporter from Lactobacillus sakei subsp. sakei 23K
24% identity, 58% coverage
- Catabolism of N-acetylneuraminic acid, a fitness function of the food-borne lactic acid bacterium Lactobacillus sakei, involves two newly characterized proteins
Anba-Mondoloni, Applied and environmental microbiology 2013 - “...In addition, two genes located downstream from nanK, lsa1644 and lsa1645, are involved in the catabolism of sialic acid in L. sakei 23K, as a L. sakei 23K...”
- “...strain lacking nan RV6030 RV6031 RV6032 RV6033 RV6034 23K lsa1645 23K (nanA-nanE) 23K nanK 23K nanT 23K nanR E. coli strains DH5 MG1655 JW25113 JW3192 JW3193...”
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...LSA1460 atkB Copper-transporting P-type ATPase 0.6 LSA1638 lsa1638 Putative large conductance mechanosensitive channel -1.0 -0.8 LSA1645 lsa1645 Putative Na(+)/(+) antiporter 1.4 D LSA1699 mntH2 Mn(2+)/Fe(2+) transport protein -0.6 LSA1703 lsa1703 Putative Na(+)/H(+) antiporter -1.2 LSA1704 lsa1704 Putative calcium-transporting P-type ATPase -0.8 LSA1735 lsa1735 Putative cobalt ABC...”
- Identification of Lactobacillus sakei genes induced during meat fermentation and their role in survival and growth
Hüfner, Applied and environmental microbiology 2007 - “...enzyme of bacterial cell wall synthesis, and gene LSA1645 (clone ici15) may also be involved in this activity. UppS catalyzes the consecutive condensation of...”
- “...nucleotide sugars used for EPS production (61, 67). LSA1645 codes for a hypothetical protein containing a conserved domain belonging to the ErfK-YbiS-YhnG...”
Mhun_0841 sodium/hydrogen exchanger from Methanospirillum hungatei JF-1
25% identity, 58% coverage
- Complete genome sequence of Methanospirillum hungatei type strain JF1
Gunsalus, Standards in genomic sciences 2016 - “...is predicted to be sodium dependent. Three genes encode Na +/ H + antiporters (Mhun_0680, Mhun_0841, Mhun_2803) that might maintain ion balance where the last differs by also possessing a Trk domain. Cell biosynthesis The genome of M. hungatei encodes an acetyl-CoA synthase/CO dehydrogenase complex (Cdh;...”
BB0447 Na+/H+ antiporter (napA) from Borrelia burgdorferi B31
21% identity, 50% coverage
- Weak Organic Acids Decrease Borrelia burgdorferi Cytoplasmic pH, Eliciting an Acid Stress Response and Impacting RpoN- and RpoS-Dependent Gene Expression
Dulebohn, Frontiers in microbiology 2017 - “...a favorable pH i . These include: (i) putative coupled proton exchange with cations (BB0637BB0638, BB0447, BB0724BB0725, BB0380) and Na + /H + antiporters (BB0637BB0638); (ii) transporting neutralizing buffering molecules (polyamines: BB0639BB0640); (iii) amino acid deaminases (BB0841BB0843); (iv) and proton export via a V o V...”
- Two Different Virulence-Related Regulatory Pathways in Borrelia burgdorferi Are Directly Affected by Osmotic Fluxes in the Blood Meal of Feeding Ixodes Ticks
Bontemps-Gallo, PLoS pathogens 2016 - “...2+ transport system ( bb0164 ), the three Na + /H + antiporter systems ( bb0447 and nhaC-1 , nhaC-2 ) and the Mg 2+ uptake system ( mgtE , bb0380 ). The expression of both nhaC-1 and nhaC-2 increased 10-fold at 250 mOsm osmolarity, suggesting...”
- “...during nymph feeding ( Fig 8B ). The three Na + /H + antiporters ( bb0447 , nhaC-1 , nhaC-2 ) were induced during the feeding, increasing 8.6-fold, 2.1-fold and 2.7-fold respectively ( Fig 8B ). The expression of bb0447 and nhaC-1 in replete ticks returned...”
- Stage-specific global alterations in the transcriptomes of Lyme disease spirochetes during tick feeding and following mammalian host adaptation
Iyer, Molecular microbiology 2015 - “...cyclic-di-GMP phosphodiesterase, PdeA bb0384 basic membrane protein C, BmpC + + bb0400 hypothetical protein + bb0447 Na+/H+ antiporter, NapA + bb0467 Multicopper polyphenol oxidase (laccase), putative + + bb0548 DNA polymerase I, PolA + + bb0563 hypothetical protein + + bb0565 purine-binding chemotaxis protein, CheW-2 +...”
- Analysis of the RpoS regulon in Borrelia burgdorferi in response to mammalian host signals provides insight into RpoS function during the enzootic cycle
Caimano, Molecular microbiology 2007 - “...bbq65 bba01 bbg11 bbg30 bbb09 bbl29 bb0400 bbg26 bbi43 bb0447 bb0548 bbq58 bb0116 Descriptiona Gene ID Table 1. cont. lp56 lp54 lp28-2 lp28-2 cp26 cp32-8...”
- “...contrast, only a handful of borrelial genes (bb0116, bb0447, bb0548, bb0728, bb0812 and bba34) with predicted roles in spirochete metabolism and/or physiology...”
- Profiling of temperature-induced changes in Borrelia burgdorferi gene expression by using whole genome arrays
Ojaimi, Infection and immunity 2003 - “...product (homologous gene) Paralogous family Fold induction BBS12 BB0447 BBN25 BB0218 BBO25 BB0237 BBH02 BBQ03 BB0377 BBA04 BBK32 BBL23 BB0751 BB0017 BBQ28 BBS14...”
PG1792 sodium/hydrogen antiporter from Porphyromonas gingivalis W83
28% identity, 45% coverage
- Studies of the extracytoplasmic function sigma factor PG0162 in Porphyromonas gingivalis
Dou, Molecular oral microbiology 2016 - “...1.47E-05 PG1225 ABC transporter, ATP-binding protein 1.6 4.85E-09 PG1760 ABC transporter, ATP-binding protein 1.6 6.10E-13 PG1792 sodium/hydrogen antiporter 2.2 1.26E-24 PG1946 ABC 3 transporter family protein 1.6 4.53E-10 PG2218 potassium transporter peripheral membrane component 1.8 6.77E-07 PG2219 potassium uptake protein TrkH 1.6 6.60E-08 stress related protein...”
XP_006355520 cation/H(+) antiporter 15-like from Solanum tuberosum
24% identity, 42% coverage
YP_001672903 sodium/hydrogen exchanger from Shewanella halifaxensis HAW-EB4
27% identity, 66% coverage
CHX18_ARATH / Q9FFR9 Cation/H(+) antiporter 18; Protein CATION/H+ EXCHANGER 18; AtCHX18 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
AT5G41610 ATCHX18; monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
26% identity, 49% coverage
- function: May operate as a cation/H(+) antiporter.
- Molecular Responses of Red Ripe Tomato Fruit to Copper Deficiency Stress
Romero, Plants (Basel, Switzerland) 2023 - “...transporter 2 (COPT2) 6.22 2.64 0.0010 AT5G59030 Solyc08g081810 Cation/H+ exchanger 18 (CHX18) 4.97 2.31 0.0088 AT5G41610 Solyc09g082870 Calcium-translocating P-type ATPase 4.81 2.27 0.0004 AT3G63380 Solyc09g014990 WRKY DNA-binding protein 2 (WRKY2) 4.80 2.26 0.0000 AT5G56270 Solyc03g111725 Unknown protein 4.78 2.26 0.0017 None Solyc08g081620 Glycosyl hydrolase 9B14 (GH9B14)...”
- NHX Gene Family in Camellia sinensis: In-silico Genome-Wide Identification, Expression Profiles, and Regulatory Network Analysis
Paul, Frontiers in plant science 2021 - “...is essential for K + nutrition, K + /Na + selectivity, and salt tolerance. CHX18 (AT5G41610), CHX17 (AT4G23700), and CHX15 (AT2G13620) are all involved in regulation of pH and are members of the putative Na + /H + antiporter family ( Berardini et al., 2015 )....”
- Proteomic and Bioinformatic Profiling of Transporters in Higher Plant Mitochondria
Møller, Biomolecules 2020 - “...experimentallyAt1g01790 K + efflux antiporter 1, chloroplastic and At2g28180Cation/H + antiporter 8and one was predicted (At5g41610 cation-H + antiporter ( Table S1 , UniProt)). Arabidopsis mitochondria also contain three members of The Monovalent Cation: Proton Antiporter-2 (CPA2) Family. At5g41610 (Cation/H + antiporter 18) was predicted but...”
- A Small RNA-Mediated Regulatory Network in Arabidopsis thaliana Demonstrates Connectivity Between phasiRNA Regulatory Modules and Extensive Co-Regulation of Transcription by miRNAs and phasiRNAs
Vargas-Asencio, Frontiers in plant science 2019 - “...Curculin-like (mannose-binding) lectin family protein 393 Chen etal. (2007) Chr5 16640239 16640870 . gene - AT5G41610 Cation/H+ exchanger 18 631 Howell etal. (2007) Chr5 16640239 16640870 . gene + AT5G41612 Natural antisense transcript overlaps with AT5G41610 631 Chr5 17560854 17561363 . gene + AT5G43725 Other RNA...”
- Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells
Pertl-Obermeyer, PloS one 2018 - “...19 (CHX19) 17 9 8 at3g17630 PM cation/H + exchanger 18 (CHX18) 9 7 2 at5g41610 PM heavy metal atpase 2 (HMA2) 1 1 0 at4g30110 PM sodium:hydrogen antiporter 1 (NHD1) 2 2 0 at3g19490 P 34.14 transport.unspecified cations 399 263 136 ADP/ATP carrier 2 (AAC2)...”
- “...(at1g78000) 5 = Na + /cation transporter HKT1 (at4g10310) 6 = cation/H + exchanger CHX18/19 (at5g41610, at3g17630) 7 = P-type H + ATPase, PM H + ATPase (at5g57350). Cell regions with abundant charasomes are also significantly enriched in cortical mitochondria [ 3 , 18 ]. Accordingly,...”
- Transcriptome Analysis of Al-Induced Genes in Buckwheat (Fagopyrum esculentum Moench) Root Apex: New Insight into Al Toxicity and Resistance Mechanisms in an Al Accumulating Species
Xu, Frontiers in plant science 2017 - “...transport comp56979_c0_seq1 1.88 AT4G24120 YSL/YSL3/YSL2 Iron transport comp66023_c0_seq1 1.83 AT5G53550 YSL3 Iron transport comp74236_c0_seq1 1.03 AT5G41610 CATION/H+ EXCHANGER 18 K + transport comp9872_c0_seq1 1.72 AT2G27240 ALMT Malate transport comp32725_c0_seq2 1.53 AT1G08440 ALMT Malate transport comp26205_c0_seq1 4.08 AT5G64560 MGT9 Mg 2+ transport comp25699_c0_seq1 3.77 AT5G64560 MGT9 Mg...”
- Transporters involved in pH and K+ homeostasis affect pollen wall formation, male fertility, and embryo development
Padmanaban, Journal of experimental botany 2017 - “...as described before ( Leydon et al. , 2015 ). Accession numbers CHX17 (At4g23700), CHX18 (At5g41610), and CHX19 (At3g17630) Results chx17chx18chx19 mutants show reduced seed set Vegetative growth and flower development of chx17chx18chx19 plants were similar to those of the wild type ( Fig. 1A-i ;...”
- Between-species differences in gene copy number are enriched among functions critical for adaptive evolution in Arabidopsis halleri
Suryawanshi, BMC genomics 2016 - “...0.07 0.36 257365_x_at c,d At2g26020 PDF1.2b Plant defensin 1.2b 0.83 0.05 0.02 0.69 249255_at c At5g41610 CHX18 Cation/H + exchanger, CPA2 family 0.77 0.63 0.00 0.01 263838_at c,d At2g36880 MAT3** Methionine adenosyltransferase 3 0.74 0.45 0.00 0.01 247128_at At5g66110 HIPP27 Putative metallochaperone-like protein 0.73 0.35 0.00...”
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NP_001318723 cation/H+ exchanger 18 from Arabidopsis thaliana
27% identity, 42% coverage
AMHT_ALKPO / D3FSJ3 Ammonium/H(+) antiporter subunit AmhT from Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4) (Bacillus pseudofirmus) (see 2 papers)
TC 2.A.37.5.1 / O50576 AmhT, component of The bidirectional K+/NH4+ transporter, AmhT (ammonium homeostasis transporter) from Bacillus pseudofirmus (see paper)
31% identity, 50% coverage
- function: Ammonium/proton antiporter that mediates the efflux of ammonium ions. Can also transport potassium or rubidium, but not sodium or lithium.
subunit: Interacts with AmhM.
disruption phenotype: Deletion affects endospore formation and germination. - substrates: Ammonium, K+
YP_001917064 sodium/hydrogen exchanger from Natranaerobius thermophilus JW/NM-WN-LF
27% identity, 48% coverage
L21SP2_0865 cation:proton antiporter from Salinispira pacifica
25% identity, 49% coverage
- Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat
Ben, Standards in genomic sciences 2015 - “...encoding phosphocarrier proteins (L21SP2_2173 and L21SP2_3294), enzyme I (L21SP2_1343 and L21SP2_3295), and enzyme II (L21SP2_0100, L21SP2_0865, L21SP2_3408, and L21SP2_3409) of a putative PTS transport mechanism were detected. In addition, genes encoding a TRAP-type dicarboxylate transporter (L21SP2_1277 and L21SP2_1278) were present. The assimilation of peptides, which had...”
XP_021757591 cation/H(+) antiporter 18-like from Chenopodium quinoa
26% identity, 44% coverage
- Salinity Effects on Guard Cell Proteome in Chenopodium quinoa
Rasouli, International journal of molecular sciences 2021 - “...metabolite XP_021738293 mitochondrial dicarboxylate/tricarboxylate transporter DTC metabolite XP_021756032 mitochondrial phosphate carrier protein 3, mitochondrial metabolite XP_021757591 cation/H + antiporter 18-like cation XP_021736780 plastidic ATP/ADP-transporter-like Misc XP_021738681 V-type proton ATPase subunit a3-like ATPases XP_021730105 V-type proton ATPase subunit C-like ATPases XP_021761683 ATPase 11, plasma membrane-type-like ATPases XP_021765334...”
BH2844 Na+/H+ antiporter from Bacillus halodurans C-125
29% identity, 55% coverage
PAB1247 Na+/H+ antiporter from Pyrococcus abyssi GE5
27% identity, 61% coverage
KHTU_BACSU / O07536 K(+)/H(+) antiporter subunit KhtU from Bacillus subtilis (strain 168) (see 3 papers)
TC 2.A.37.5.2 / O07536 Putative uncharacterized protein yhaU (YhaU protein), component of The K +/H+ antiporter, YhaTU from Bacillus subtilis (see 4 papers)
25% identity, 61% coverage
- function: Potassium/proton antiporter that mediates the efflux of potassium ions from the cell (PubMed:17679694). Can also mediate rubidium/proton antiport, but has no permeability for sodium or lithium ions. In the absence of KhtT, does not have antiport activity, but can catalyze potassium efflux (PubMed:14987767, PubMed:17679694). Involved in protection of the cell from methylglyoxal, a toxic by-product of glycolysis, via activation by S-lactoyl-BSH of the antiporter activity, leading to cytoplasmic acidification and methylglyoxal resistance (PubMed:24330391).
subunit: The transporter is composed of the integral membrane protein KhtU and the regulatory protein KhtT.
disruption phenotype: Deletion mutant is more sensitive to methylglyoxal than wild-type. - substrates: H+, K+
LOC102595577, XP_006355611 cation/H(+) antiporter 18-like from Solanum tuberosum
26% identity, 42% coverage
LOC103931079 LOW QUALITY PROTEIN: cation/H(+) antiporter 20 from Pyrus x bretschneideri
27% identity, 44% coverage
slr0415 Na(+)/H(+) antiporter from Synechocystis sp. PCC 6803
30% identity, 40% coverage
- Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB
Selim, Science advances 2021 - “...transporter MgtE ( slr1216 ), the sodium/H + antiporters NhaS2 and NhaS5 ( sll0273 and slr0415 , respectively), and the glutamate-Na + symporter ( slr0625 ) were identified as c-di-AMPbinding proteins. In addition to SbtB, the identification of these potential c-di-AMPdependent transporters implied that c-di-AMP may...”
- Regulation Mechanism Mediated by Trans-Encoded sRNA Nc117 in Short Chain Alcohols Tolerance in Synechocystis sp. PCC 6803
Bi, Frontiers in microbiology 2018 - “...been reported involved in cyanobacterial tolerance to various kinds of environmental stresses previously. For example, slr0415 encoding Na(+)/H(+) antiporter was found related to salt stress response and internal pH regulation of the Synechocystis ( Elanskaya et al., 2002 ), and the sll0272 mutant was sensitive to...”
- Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites
Pinto, DNA research : an international journal for rapid publication of reports on genes and genomes 2015 - “...drug efflux system protein, the N4 site overlaps the 5 region of an ORF ( slr0415 ) putatively encoding a Na + /H + antiporter and the N9 site corresponds to the ORF located immediately upstream and in the same direction as an ORF ( sll0182...”
- Global transcriptional profiles of the copper responses in the cyanobacterium Synechocystis sp. PCC 6803
Giner-Lamia, PloS one 2014 - “...acid transport system sll1270 bgtB 0.29 ABC-type Bgt permease for basic amino acids and glutamine slr0415 napA 0.29 Na+/H+ antiporter slr0369 envD 0.28 RND multidrug efflux transporter slr0875 mscL 0.25 Large-conductance mechanosensitive channel slr0096 0.24 Low affinity sulfate transporter sll1406 fhuA 0.24 Ferrichrome-iron receptor sll0224 0.23...”
- Iron deprivation in Synechocystis: inference of pathways, non-coding RNAs, and regulatory elements from comprehensive expression profiling
Hernández-Prieto, G3 (Bethesda, Md.) 2012 - “...1.60 0.12 0.17 0.60 5.8810 5 0.98 sll0095-as1 1.34 1.28 0.19 0.17 0.46 2.3110 6 slr0415 Na + /H+ antiporter 1.02 1.15 0.04 0.10 0.46 1.6510 4 0.97 slr0415-as3 1.49 1.35 0.63 0.62 1.07 5.3710 7 slr0727 Unknown protein 1.32 1.37 0.34 0.01 0.42 1.1310 5...”
- Two members of a network of putative Na+/H+ antiporters are involved in salt and pH tolerance of the freshwater cyanobacterium Synechococcus elongatus
Billini, Journal of bacteriology 2008 - “...24, 65) (encoded by slr1727, sll0273, sll0689, slr1595, slr0415, and sll0556 and designated NhaS1, NhaS2, NhaS3, NhaS4, NhaS5, and NhaS6, respectively), as well...”
- Polymerase chain reaction-based mutageneses identify key transporters belonging to multigene families involved in Na+ and pH homeostasis of Synechocystis sp. PCC 6803
Wang, Molecular microbiology 2002 (PubMed)- “...Na+/H+ antiporters (slr1727, sll0273, sll0689, slr1595 and slr0415) and seven cation ATPases (sll1614, sll1920, slr0671-72, slr0822, slr1507-08-09, slr1728- 29...”
- “...(nhaS1)b sll0273 (nhaS2) sll0689 (nhaS3) slr1595 (nhaS4) slr0415 (nhaS5) Putative cation ATPase sll0671-72 slr0822 sll1614 (pma1) slr 1728-29 (kdpA, B)...”
- Functional analysis of the Na+/H+ antiporter encoding genes of the cyanobacterium Synechocystis PCC 6803
Elanskaya, Biochemistry. Biokhimiia 2002 (PubMed)- “...(slr1727), nhaS3 (sll0689), nhaS4 (slr1595), and nhaS5 (slr0415) in salt stress response and internal pH regulation of the cyanobacterium Synechocystis PCC...”
- “...remaining three proteins (Sll0689 = NhaS3; Slr1595 = NhaS4; Slr0415 = NhaS5) are obviously of prokaryotic type (Fig. 1). For two of these putative transport...”
- More
CHX15_ARATH / Q9SIT5 Cation/H(+) antiporter 15; Protein CATION/H+ EXCHANGER 15; AtCHX15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
AT2G13620 ATCHX15; monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
25% identity, 46% coverage
- function: May operate as a cation/H(+) antiporter.
- Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)
Wang, Frontiers in plant science 2021 - “...). CHX15, CHX21, and CHX23 belong to same subfamily of cation exchangers [see UniProtKB - Q9SIT5 4 ; ( Chanroj et al., 2011 )]; therefore, a similar role can be expected. This is an interesting observation and a good gene of interest for a detailed exploration...”
- Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.)
Sinha, Frontiers in plant science 2015 - “...protein 36 C.cajan_30211 7.13 0.86 1.00 Q9FKG6 U-box domain-containing protein 52 C.cajan_46779 7.47 0.03 0.60 Q9SIT5 Cation/H(+) antiporter 15 C.cajan_08737 7.70 1.03 0.24 I1JEJ0 Uncharacterized protein C.cajan_13768 4.56 0.58 0.04 Q8LGG8 Universal stress protein A-like protein C.cajan_23080 6.19 1.70 3.10 Q57951 Universal stress protein C.cajan_29830 11.40...”
- Identification of QTLs and allelic effect controlling lignan content in sesame (Sesamum indicum L.) using QTL-seq approach
Kim, Frontiers in genetics 2023 - “...inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein SIN_1018438 2 0 0 0 0 5 3 AT2G13620 cation/hydrogen exchanger 15 (CHX15) SIN_1018440 1 5 4 0 0 0 3 AT4G15550 indole-3-acetate beta-D-glucosyltransferase (IAGLU) SIN_1018441 0 1 0 0 0 0 6 AT4G12490 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S...”
- NHX Gene Family in Camellia sinensis: In-silico Genome-Wide Identification, Expression Profiles, and Regulatory Network Analysis
Paul, Frontiers in plant science 2021 - “...nutrition, K + /Na + selectivity, and salt tolerance. CHX18 (AT5G41610), CHX17 (AT4G23700), and CHX15 (AT2G13620) are all involved in regulation of pH and are members of the putative Na + /H + antiporter family ( Berardini et al., 2015 ). Figure 7 Functional interaction networks...”
- Meta-analysis of the expression profiles of the Arabidopsis ESCRT machinery
Richardson, Plant signaling & behavior 2011 - “...ubiquitin-protein ligase activity AT2G14700 Unknown function AT2G13620 Member of Putative Na+/H+ antiporter family AT1G51750 Transposable element gene (c)201...”
- Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting
Chanroj, The Journal of biological chemistry 2011 - “...and AtNHX12 (At5g27150, At3g05030) cluster within the CPA1 family. AtCHX genes identified by AGI no. At2g13620 (CHX15), At1g64170 (CHX16), At4g23700 (CHX17), At5g41610 (CHX18), At3g17630 (CHX19), and At3g53720 (CHX20) encode proteins of 86.991.5 kDa ( supplemental Fig. S1 ). Alignment was processed by ClustalX version 2.0. Values...”
CHX16_ARATH / Q1HDT3 Cation/H(+) antiporter 16; Protein CATION/H+ EXCHANGER 16; AtCHX16 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
AT1G64170 ATCHX16 (CATION/H+ EXCHANGER 16); monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
27% identity, 36% coverage
- function: May operate as a cation/H(+) antiporter.
- A general non-self response as part of plant immunity
Maier, Nature plants 2021 - “...were mutants of genes encoding a chitinase family protein (AT2G43620), a putative Na+/H+ anti-porter (CHX16, AT1G64170), an O-methyltransferase family protein (IGMT3, AT1G21110), a cell-wall bound peroxidase (PRX71, AT5G64120), mildew resistance locus 12 (MLO12, AT2G39200), and a glutathione S-transferase (GSTF6, AT1G02930) as well as CYP71A12 (AT2G30750). With...”
- Forward genetic screens identify a role for the mitochondrial HER2 in E-2-hexenal responsiveness
Scala, Plant molecular biology 2017 - “...P5CDH, D1-pyrroline-5-carboxylate dehydrogenase; SSADH, succinic semialdehyde dehydrogenase; GLYR, glyoxylate reductase; SSADH-AC, succinic semialdehyde dehydrogenase acetylating. At1g64170, At1g32780, At1g77120, At5g43940, At4g22110 and At5g42250 are the six best hits with HER2 as a query against the Arabidopsis protein database ( http://www.arabidopsis.org ). The tree was generated with CLC...”
- Transcriptome profiling of genes involved in induced systemic salt tolerance conferred by Bacillus amyloliquefaciens FZB42 in Arabidopsis thaliana
Liu, Scientific reports 2017 - “...AT3G23250, AT4g26200, AT1g17420, AT4G39210, AT4G23700, AT1g29910, AT2G05070, ATCG00120, ATCG00580, AT2G39800, AT2G05100, AT2G34420, AT3G27690, AT5G54270, AT4G39770, AT1G64170, AT1G14540, AT1G70290, AT3g14440, AT4G13250, AT1G78090, AT4G36110, AT5G23370, AT3G24500, AT3G03480, AT4G08390, AT1G05560, AT5G51720, AT4G32810, AT3G12580, AT3G04800, AT1G64900, AT3G44990, and AT1G10070). Bars represent the meanSD based on three replicates. Discussion Previous studies...”
- Between-species differences in gene copy number are enriched among functions critical for adaptive evolution in Arabidopsis halleri
Suryawanshi, BMC genomics 2016 - “...0.75 0.00 0.00 260913_at d At1g02500 SAMS1 S-adenosylmethionine synthetase 3 0.85 0.44 0.00 0.03 262324_at At1g64170 CHX16 Cation/H + exchanger, CPA2 family 0.84 0.38 0.07 0.36 257365_x_at c,d At2g26020 PDF1.2b Plant defensin 1.2b 0.83 0.05 0.02 0.69 249255_at c At5g41610 CHX18 Cation/H + exchanger, CPA2 family...”
- Impairment of Respiratory Chain under Nutrient Deficiency in Plants: Does it Play a Role in the Regulation of Iron and Sulfur Responsive Genes?
Vigani, Frontiers in plant science 2015 - “...family protein at5g48180 NSP5 (NITRILE SPECIFIER PROTEIN 5) at3g47960 Proton-dependent oligopeptide transport (POT) family protein at1g64170 ATCHX16 (CATION/H+ EXCHANGER 16 at5g48850 ATSDI1 (SULFUR DEFICIENCY-INDUCED 1) at3g12520 SULTR4;2; sulfate transmembrane transporter at1g04770 Male sterility MS5 family protein at1g75280 Isoflavone reductase, putative at4g13430 IIL1 (ISOPROPYL MALATE ISOMERASE LARGE...”
- Investigating the toxicity, uptake, nanoparticle formation and genetic response of plants to gold
Taylor, PloS one 2014 - “...MTPc3 Cation efflux family protein 4.4 Down At4g30120 HMA3 Heavy metal ATPase 3 3.2 Down At1g64170 ATCHX16 Sodium: hydrogen antiporter 3.1 Down At5g26820 ATIREG3 Iron regulated protein 3 3.0 Down At1g80830 NRAMP1 Manganese transmembrane transporter 4.6 Up At3g51860 CAX3 Calcium cation antiporter Expression of FIT1, a...”
- Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting
Chanroj, The Journal of biological chemistry 2011 - “...(At5g27150, At3g05030) cluster within the CPA1 family. AtCHX genes identified by AGI no. At2g13620 (CHX15), At1g64170 (CHX16), At4g23700 (CHX17), At5g41610 (CHX18), At3g17630 (CHX19), and At3g53720 (CHX20) encode proteins of 86.991.5 kDa ( supplemental Fig. S1 ). Alignment was processed by ClustalX version 2.0. Values indicate the...”
- Testing for effects of recombination rate on nucleotide diversity in natural populations of Arabidopsis lyrata
Wright, Genetics 2006 - “...AT1G36730 AT1G42470 AT1G42990 AT1G59720 AT1G62310 AT1G62390 AT1G62520 AT1G64170 AT1G65450 AT1G68520 AT1G68530 AT1G72390 AT1G74600 AT1G76550 1 1 1 1 1 1 1...”
LIMLP_17535 cation:proton antiporter from Leptospira interrogans serovar Manilae
26% identity, 82% coverage
YP_001672941 sodium/hydrogen exchanger from Shewanella halifaxensis HAW-EB4
24% identity, 59% coverage
Cp258_0622 cation:proton antiporter from Corynebacterium pseudotuberculosis 258
28% identity, 62% coverage
LIC12221 cation:proton antiporter from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
27% identity, 49% coverage
LOC105033828 cation/H(+) antiporter 20 from Elaeis guineensis
27% identity, 32% coverage
- Genes, pathways and networks responding to drought stress in oil palm roots
Wang, Scientific reports 2020 - “...member 1 Up LOC105049578 Amino acid transporter ANT1 Up LOC105037902 Receptor-like protein kinase HSL1 Down LOC105033828 Cation/H(+) antiporter 20 Up LOC105039277 Plasma membrane ATPase Down LOC105052943 Plasma membrane ATPase 4 Up LOC105047848 Potassium channel AKT1 Up LOC105040536 ABC transporter G family member 42 Down Reactive nitrogen...”
LOC103844110 cation/H(+) antiporter 23, chloroplastic from Brassica rapa
25% identity, 43% coverage
LIMLP_11025 cation:proton antiporter from Leptospira interrogans serovar Manilae
27% identity, 49% coverage
LOC100264818 cation/H(+) antiporter 20 from Vitis vinifera
26% identity, 44% coverage
BTH_II1229 sodium/hydrogen exchanger from Burkholderia thailandensis E264
27% identity, 60% coverage
Synpcc7942_2359 Na+/H+ antiporter from Synechococcus elongatus PCC 7942
26% identity, 57% coverage
- The Freshwater Cyanobacterium Synechococcus elongatus PCC 7942 Does Not Require an Active External Carbonic Anhydrase
Kupriyanova, Plants (Basel, Switzerland) 2024 - “...genes Synpcc7942_1468, Synpcc7942_1469, Synpcc7942_1473, and Synpcc7942_1474); potential Na + /H + antiporters Nha17 (Synpcc7942_0811, Synpcc7942_1264, Synpcc7942_2359, Synpcc7942_0546, Synpcc7942_0307, Synpcc7942_2394, and Synpcc7942_2186); as well as the PxcA proton pump (Synpcc7942_0991). The following genes were selected for this study: (1) ndhD5 (Synpcc7942_1473), which encodes one of the Mnh...”
DVDV_0267 potassium channel family protein from Desulfovibrio sp. DV
34% identity, 20% coverage
XP_006343906 cation/H(+) antiporter 18-like from Solanum tuberosum
26% identity, 33% coverage
LOC103931080 cation/H(+) antiporter 20 from Pyrus x bretschneideri
29% identity, 49% coverage
SO_0695 glutathione-regulated potassium-efflux system protein KefC, putative from Shewanella oneidensis MR-1
24% identity, 82% coverage
XP_015084067 cation/H(+) antiporter 18-like from Solanum pennellii
26% identity, 44% coverage
NHAS3_SYNY3 / Q55190 High-affinity Na(+)/H(+) antiporter NhaS3; Sodium/proton antiporter NhaS3 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see 3 papers)
TC 2.A.37.2.4 / Q55190 The high-affinity (Km(Na+)=0.7 mM) Na+(Li+):H+ thylakoid membrane antiporter, NhaS3 (essential for growth; promotes Na+ resistance; expressed in the presence of high CO2 concentrations; under circadian control (see paper)
sll0689 Na/H+ antiporter from Synechocystis sp. PCC 6803
25% identity, 60% coverage
- function: Na(+)/H(+) antiporter that transports sodium from the cytoplasm into the thylakoid lumen in exchange for protons. Contributes to sodium homeostasis and tolerance. Has also Li(+)/H(+) antiport activity under K(+)-free conditions, but not under K(+)-rich conditions.
- substrates: H+, Li+, Na+
- Global transcriptional and circadian regulation in a halotolerant cyanobacterium Halothece sp. PCC7418
Waditee-Sirisattha, Scientific reports 2022 - “...similarities (57% and 66% identities, respectively) with an essential Na + /H + antiporter, NhaS3 (Sll0689), found in Synechocystis sp. PCC6803. NhaS3 is thought to be associated with salt tolerance in Synechocystis sp . PCC6803 10 . The upregulation of the multi-cistronic operon CPA3, which is...”
- The drnf1 Gene from the Drought-Adapted Cyanobacterium Nostoc flagelliforme Improved Salt Tolerance in Transgenic Synechocystis and Arabidopsis Plant
Cui, Genes 2018 - “...F, 5-ATTACGTGAAGGGCACCAAG-3; sll1566 -R, 5-TTAATTTTCCCTGCCAGTCG-3), nhaS1 ( slr1727 -F, 5-ATTGCCTTTCCCCTTTCCTA-3; slr1727 -R, 5-AAATAGGCTCTCCCCTTCCA-3), nhaS3 ( sll0689 -F, 5-TTGCCTCTGGCAGACTTTTT-3; sll0689 -R, 5-AACCGGTAACCACCTTACCC-3), rre37 ( sll1330 -F: 5-GCCGTGATTGATTCTGACCT-3; sll1330 -R, 5-AAAATTCCTGCATGCCAAAG-3), sigB ( sll0306 -F, 5-ATGGTAACAGTGACAGTTAT-3; sll0306 -R, 5-GCTTCAATCATTTTCCGTTT-3). Transcriptional levels of target genes were normalized to those...”
- Function and evolution of channels and transporters in photosynthetic membranes
Pfeil, Cellular and molecular life sciences : CMLS 2014 - “...78 , 79 , 82 , 84 ] Na + /H + antiporter NhaS3 Synechocystis sll0689 Na + , H + Essential gene. Balance of Na + /K + ratio. Reduce toxic effects of Na + in the cytosol and of lumen acidification [ 86 ]...”
- Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystis sp. PCC 6803
Zhu, Biotechnology for biofuels 2013 - “...Cyanophycin synthetase Transport and binding proteins sll0374 1.65 1.90 2.83 Urea transport system ATP-binding protein sll0689 2.21 1.63 3.28 Na+/H+antiporter sll0759 3.11 1.56 6.87 ABC transporter ATP-binding protein sll1041 3.74 2.50 3.43 Similar to sulfate transport ATP-binding protein CysA sll1154 1.76 2.80 3.98 NorA sll1164 6.60...”
- “...iii. Transporters: transcriptomics analysis identified 19 membrane transporters were up-regulated. Among them only two genes, sll0689 and slr1512 which were in the same operon with butanol-induced slr1515 , were identified in the previous proteomics analysis [ 26 ]. Interestingly, the up-regulated transporters involved a wide range...”
- Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803
Liu, Biotechnology for biofuels 2012 - “...guanyltransterase Ssr2857 1.51 Mercuric transport protein periplasmic component precursor Sll1394 1.53 Methionine sulfoxide reductase A Sll0689 1.85 Na/H + antiporter Sll0493 1.77 Na-activated K transporter subunit KtrA Slr0891 1.56 1.51 N-acetylmuramoyl-L-alanine amidase Sll0223 2.22 2.08 NAD (P) H-quinone oxidoreductioase subunit 2 Sll1262 1.52 NAD (P) H-quinone...”
- “...involved in cation transporting (Slr2131, Sll0672), two involved in Na + and K + transporting (Sll0689, Sll0493), one involved in mercuric transporting (Ssr2857), were up-regulated (Table 1 ). In Synechocystis sp. PCC 6803, the slr004 0, slr0041 , slr0043 , and slr0044 genes, forming an operon...”
- RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803
Wang, Biotechnology for biofuels 2012 - “...they shared some similarity in terms of substrate specificity as two of previously identified transporters, Sll0689 as a sodium-dependent transporter and Slr1295 as an iron transporter. Early studies have found that many microbes can modify their cell membrane and envelope to increase tolerance to ethanol [...”
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...transporter b0855 PotG b0856 PotH b0857 PotI P. mari ABX75857 GpgS Glucosylglycerate synthetase ABX75858 GpgP sll0689 NhaS3 Na + /H + antiporter sll0493 KtrA Predominant K + transporter playing a major slr1509 KtrB role in K + uptake under osmotic stress slr1508 KtrE slr1728 KdpA High-affinity...”
- “...Organism b3404 EnvZ SYNW0807 SYNW0807-0808 1 E. coli b3405 OmpR SYNW0808 SYNW0807-0808 0.99 E. coli sll0689 NhaS3 SYNW0157 0.99 PCC6803 sll0493 KtrA SYNW2169 SYNW2165-2170 0.56 PCC6803 slr1509 KtrB SYNW2168 SYNW2165-2170 0.99 PCC6803 slr1508 KtrE SYNW0663 SYNW0663-0667 0.99 PCC6803 b2741 38 SYNW1621 1 E. coli sll0306 RpoD...”
- Identification and characterization of the Na+/H+ antiporter Nhas3 from the thylakoid membrane of Synechocystis sp. PCC 6803
Tsunekawa, The Journal of biological chemistry 2009 - “...coli--For heterologous expression in E. coli, the nhaS3 (sll0689) gene was isolated from chromosomal DNA by PCR using KpnI site-containing forward primer...”
- More
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...domain of AtKEA3, EcNhaA, EcKefB (P45522) EcKefC, TtNapA, Bacillus cereus GerN (BcGerN, Q9KI10), Synechocystis NhaS3 (Q55190) and NhaS4 (Q5N3F5), Arabidopsis AtCHX17 (Q9SUQ7), AtKEA2 and Saccharomyces cerevisiae KHA1 (P40309) were performed using ClustalOmega. KEA3 co-expression analysis was performed by determining Pearson correlation coefficients of all Arabidopsis genes...”
BC5041 Na+/H+ antiporter NapA from Bacillus cereus ATCC 14579
26% identity, 55% coverage
BF9343_3100 cation:proton antiporter from Bacteroides fragilis NCTC 9343
25% identity, 50% coverage
- Multi-omics approach for understanding the response of Bacteroides fragilis to carbapenems
Zholdybayeva, Heliyon 2024 - “...BF9343_0082 Gluconate 5-dehydrogenase (gno) 1,17E+00 transporter activity BF9343_3070 Chromate transport protein 1,32E+00 proton antiporter activity BF9343_3100 cation:proton antiporter 8,55E-01 oxidoreductase activity BF9343_1855 cytochrome d ubiquinol oxidase subunit II(cydB) 1,98E+00 dipeptidase activity BF9343_1339 Dipeptidase (pepD_3) 4,98E+00 lyase activity BF9343_2473 Carboxynorspermidine/carboxyspermidine decarboxylase 1,20E+00 BF9343_1258 Pyruvate formate-lyase-activating enzyme (pflA)...”
KHA1_YEAST / P40309 K(+)/H(+) antiporter 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
TC 2.A.37.4.1 / P40309 K+:H+ antiporter, Kha1 (YJL094c) from Saccharomyces cerevisiae (Baker's yeast) (see 6 papers)
KHA1 / RF|NP_012441.1 K(+)/H(+) antiporter 1 from Saccharomyces cerevisiae
NP_012441 Kha1p from Saccharomyces cerevisiae S288C
YJL094C Kha1p from Saccharomyces cerevisiae
22% identity, 42% coverage
- function: Potassium-proton antiport
- substrates: H+, K+
- Ion antiport accelerates photosynthetic acclimation in fluctuating light environments
Armbruster, Nature communications 2014 - “...Q9KI10), Synechocystis NhaS3 (Q55190) and NhaS4 (Q5N3F5), Arabidopsis AtCHX17 (Q9SUQ7), AtKEA2 and Saccharomyces cerevisiae KHA1 (P40309) were performed using ClustalOmega. KEA3 co-expression analysis was performed by determining Pearson correlation coefficients of all Arabidopsis genes with KEA3 using the GeneCAT database ( http://genecat.mpg.de ). All genes in...”
- Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase.
Wu, The Journal of biological chemistry 2016 - GeneRIF: study reveals a novel functional role of K(+) in the binding of copper to apoFet3p and identifies a K(+)/H(+) exchanger at the secretory pathway as a new molecular factor associated with iron uptake in yeast.
- Genetic interactions among the Arl1 GTPase and intracellular Na(+) /H(+) antiporters in pH homeostasis and cation detoxification.
Marešová, FEMS yeast research 2010 (PubMed)- GeneRIF: The authors conclude that Arl1p, Kha1p and Nhx1p collaborate in survival strategies at nonoptimal pH, temperatures and cation concentrations, but work independent of each other.
- Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison.
Pribylová, Folia microbiologica 2006 (PubMed)- GeneRIF: Yeast Kha1 proteins probably belong to the same subfamily as bacterial antiporters, whereas Nhal proteins form a distinct subfamily
- Physiological characterization of Saccharomyces cerevisiae kha1 deletion mutants.
Maresova, Molecular microbiology 2005 (PubMed)- GeneRIF: important for the regulation of intracellular cation homeostasis and optimal pH control
- Whole genome duplication and enrichment of metal cation transporters revealed by de novo genome sequencing of extremely halotolerant black yeast Hortaea werneckii
Lenassi, PloS one 2013 - “...with the following S. cerevisiae S288C protein queries: Trk1 (YJL129C), Trk2 (YKR050W), Tok1 (YJL093C), Kha1 (YJL094C), Mrs7 (YPR125W), Nha1 (YLR138W), Vnx1 (YNL321W), Nhx1 (YDR456W), Ena1 (YDR040C), Ena2 (YDR039C), Ena5 (YDR038C) and Pho89 (YBR296C). a A part of N-terminal or C-terminal protein sequence might be missing. b...”
- “...Tok1 (YJL093C), Nha1 (YLR138W), Ena1 (YDR040C), Ena2 (YDR039C), Ena5 (YDR038C), Pho89 (YBR296C), Nhx1 (YDR456W), Kha1 (YJL094C), Vnx1 (YNL321W), Mrs7 (YPR125W)) and its H. werneckii ( Table 2 ) and M. graminicola (Trk1: XP_003850012.1; Tok1: XP_003847595.1; Nha1: XP_003855439.1, XP_003856011.1 and XP_003855492.1; Ena1: XP_003852150.1, XP_003854801 and XP_003850456.1; Pho89:...”
- Population genomics and transcriptional consequences of regulatory motif variation in globally diverse Saccharomyces cerevisiae strains
Connelly, Molecular biology and evolution 2013 - “...YAL049C YPL255W YNR049C YOR071C YPR119W YPL108W YNL240C YKL108W YJL094C YBR222C YGL117W YLR152C YPL167C YLR007W 0.26 0.27 0.29 0.57 0.32 0.47 0.24 0.59 0.39...”
- Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting
Chanroj, The Journal of biological chemistry 2011 - “...AtCHX15AtCHX20. FIGURE 1. Arabidopsis CHX15 to -20 are homologs of yeast KHA1. AtCHX15AtCHX20 and ScKHA1 (YJL094C) form a cluster within the CPA2 family, and ScNHX1 (YDR456W) and AtNHX12 (At5g27150, At3g05030) cluster within the CPA1 family. AtCHX genes identified by AGI no. At2g13620 (CHX15), At1g64170 (CHX16), At4g23700...”
- Alkali metal cation transport and homeostasis in yeasts
Ariño, Microbiology and molecular biology reviews : MMBR 2010 - “...12 Symporter 574 10 Channel Not known Not known Kha1 Nhx1 YJL094c YDR456w 2.A.37.4.1 2.A.36.2.1 GA LE Antiporter Antiporter 873 633 12 8 (?) K, Rb K, Rb K Na,...”
- “...the three yeast Na/H antiporters, Kha1 (encoded by YJL094c) has the highest level of similarity to bacterial sodium or potassium/proton antiporters (218, 227)....”
- The unfolded protein response is necessary but not sufficient to compensate for defects in disulfide isomerization
Kim, The Journal of biological chemistry 2009 - “...SEC28 (YIL076W) ARR4/GET3 (YDL100C) INP53 (YOR109W) KHA1 (YJL094C) RGP1 (YDR137W) ALG8 (YOR067C) GEF1 (YJR040W) PSD2 (YGR170W) YUR1 (YJL139C) SYS1 (YJL004C)...”
- “...(YMR123W) component component V-ATPase assembly KHA1 (YJL094C) cation homeostasis vesicle Golgi component component reticulum-associated degradation (ERAD) of...”
- Novel insights into iron metabolism by integrating deletome and transcriptome analysis in an iron deficiency model of the yeast Saccharomyces cerevisiae
Jo, BMC genomics 2009 - “...Ferric (and cupric) reductase Plasma membrane YKR052C 1 MRS4 0.50 -3.55 Mitochondrial carrier protein Mitochondria YJL094C 1 KHA1 0.40 -2.51 Putative H+/K+ antiporter Membrane YHL027W 1 RIM101 0.76 -2.64 Transcriptional activator required for meiosis Nucleus YDR271C 1 0.82 -2.56 Unknown, overlaps CCC2 Unknown YDR270W 1 CCC2...”
- Role of PUG1 in inducible porphyrin and heme transport in Saccharomyces cerevisiae
Protchenko, Eukaryotic cell 2008 - “...1.9 2.2 2.0 2.3 5.5 6.9 3.1 1.6 2.2 1.9 2.4 3.9 6.5 YJL094C YJL094C FET4 TRK2 7 10 2.0 2.1 2.4 2.1 2.7 3.4 2.1 3.2 YJL094C YML132W KHA1 COS3 12 4 2.0 1.9 2.4...”
- A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae
Addinall, Genetics 2008 - “...YHL002W YGL253W [YJR118C YDR315C YFR055W YGL016W YHR158C YNL238W YJL094C YAR018C YNL322C CYT2 DBP3 DEG1 DID2 DIE2 DOA1 EBS1 EDE1 ELM1 EMI1 ERG5 ERG6] FTR1...”
- More
PF0275 Na+/H+ antiporter from Pyrococcus furiosus DSM 3638
28% identity, 41% coverage
AORI_1497 cation:proton antiporter from Amycolatopsis keratiniphila
25% identity, 57% coverage
- Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Xu, BMC genomics 2014 - “...P450 lcl|Y16952.3_cdsid_CAC48370.1 85.35 AORI_1495 hmaS 4-hydroxymandelate synthase lcl|AJ223998.1_cdsid_CAA11761.1 75.56 AORI_1496 hmo 4-hydroxymandelate oxidase lcl|Y16952.3_cdsid_CAC48372.1 85.24 AORI_1497 Antipoter lcl|Y16952.3_cdsid_CAC48373.1 80 AORI_1498 vcaA Oxidase lcl|Y16952.3_cdsid_CAC48374.1 88.04 AORI_1499 vcaE Reductase lcl|Y16952.3_cdsid_CAC48375.1 84.21 AORI_1500 vcaB Aminotransferase lcl|AJ223998.1_cdsid_CAA11782.1 88.62 AORI_1501 vcaD Epimerase lcl|Y16952.3_cdsid_CAC48377.1 85.57 AORI_1502 dpgA Polyketide synthase lcl|AJ223998.1_cdsid_CAA11765.1 92.9 AORI_1503...”
A5BEW1 Uncharacterized protein from Vitis vinifera
27% identity, 39% coverage
CHX19_ARATH / Q9LUN4 Cation/H(+) antiporter 19; Protein CATION/H+ EXCHANGER 19; AtCHX19 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
AT3G17630 ATCHX19 (CATION/H+ EXCHANGER 19); monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
NP_001319577 cation/H+ exchanger 19 from Arabidopsis thaliana
26% identity, 36% coverage
- function: May operate as a cation/H(+) antiporter.
- Transcriptome analysis of Arabidopsis reveals freezing-tolerance related genes induced by root endophytic fungus Piriformospora indica
Jiang, Physiology and molecular biology of plants : an international journal of functional plant biology 2021 - “...Table S7). Seven DEGs (AT5G17850, AT3G53720, AT5G47560, AT3G17630, AT2G38170, AT3G06370, AT5G17860) encode sodium exchanger proteins, two DEGs (AT4G35090 and...”
- Dissecting the subcellular membrane proteome reveals enrichment of H+ (co-)transporters and vesicle trafficking proteins in acidic zones of Chara internodal cells
Pertl-Obermeyer, PloS one 2018 - “...at1g15500 P 34.12 transport.metal 29 19 10 cation/H + exchanger 19 (CHX19) 17 9 8 at3g17630 PM cation/H + exchanger 18 (CHX18) 9 7 2 at5g41610 PM heavy metal atpase 2 (HMA2) 1 1 0 at4g30110 PM sodium:hydrogen antiporter 1 (NHD1) 2 2 0 at3g19490 P...”
- “...5 = Na + /cation transporter HKT1 (at4g10310) 6 = cation/H + exchanger CHX18/19 (at5g41610, at3g17630) 7 = P-type H + ATPase, PM H + ATPase (at5g57350). Cell regions with abundant charasomes are also significantly enriched in cortical mitochondria [ 3 , 18 ]. Accordingly, proteins...”
- Transporters involved in pH and K+ homeostasis affect pollen wall formation, male fertility, and embryo development
Padmanaban, Journal of experimental botany 2017 - “...( Leydon et al. , 2015 ). Accession numbers CHX17 (At4g23700), CHX18 (At5g41610), and CHX19 (At3g17630) Results chx17chx18chx19 mutants show reduced seed set Vegetative growth and flower development of chx17chx18chx19 plants were similar to those of the wild type ( Fig. 1A-i ; Supplementary Fig. S1...”
- Plant-specific cation/H+ exchanger 17 and its homologs are endomembrane K+ transporters with roles in protein sorting
Chanroj, The Journal of biological chemistry 2011 - “...family. AtCHX genes identified by AGI no. At2g13620 (CHX15), At1g64170 (CHX16), At4g23700 (CHX17), At5g41610 (CHX18), At3g17630 (CHX19), and At3g53720 (CHX20) encode proteins of 86.991.5 kDa ( supplemental Fig. S1 ). Alignment was processed by ClustalX version 2.0. Values indicate the percentage of times (%) that each...”
- Pollen development and fertilization in Arabidopsis is dependent on the MALE GAMETOGENESIS IMPAIRED ANTHERS gene encoding a type V P-type ATPase
Jakobsen, Genes & development 2005 - “...At1g47980 At4g16480 At1g22760 At2g28680 At5g20710 At5g48570 At4g08670 At3g17630 At2g16750 At1g80660 At5g03620 At1g47400 At1g18280 At4g16160 15 16 17 18 19 20 21...”
- Transporters involved in pH and K+ homeostasis affect pollen wall formation, male fertility, and embryo development.
Padmanaban, Journal of experimental botany 2017 - GeneRIF: Fertility could be partially compromised by impaired pollen tube and/or sperm function as CHX19 and CHX18 are expressed in the pollen tube and sperm cell, respectively. CHX17 and CHX18 proteins may promote embryo development possibly through the endosperm where these genes are expressed.
alr0091 Na+/H+-exchanging protein from Nostoc sp. PCC 7120
23% identity, 53% coverage
slr1595 Na/H antiporter from Synechocystis sp. PCC 6803
26% identity, 44% coverage
- RNA-Seq analysis provides insights for understanding photoautotrophic polyhydroxyalkanoate production in recombinant Synechocystis Sp
Lau, PloS one 2014 - “...signal transduction slr2131 cation or drug efflux system protein 2.29 2.6 26.11 59.75 67.83 transport slr1595 Na/H antiporter 2.45 2.59 13.76 33.65 35.64 cation transport slr1912 anti-sigma F factor antagonist 1.87 2.59 75.93 141.71 196.56 regulation of transcription ssl2296 pterin-4-alpha-carbinolamine dehydratase 1.81 2.51 56.88 103.21 142.56...”
- Two members of a network of putative Na+/H+ antiporters are involved in salt and pH tolerance of the freshwater cyanobacterium Synechococcus elongatus
Billini, Journal of bacteriology 2008 - “...(19, 24, 65) (encoded by slr1727, sll0273, sll0689, slr1595, slr0415, and sll0556 and designated NhaS1, NhaS2, NhaS3, NhaS4, NhaS5, and NhaS6, respectively), as...”
- Polymerase chain reaction-based mutageneses identify key transporters belonging to multigene families involved in Na+ and pH homeostasis of Synechocystis sp. PCC 6803
Wang, Molecular microbiology 2002 (PubMed)- “...putative Na+/H+ antiporters (slr1727, sll0273, sll0689, slr1595 and slr0415) and seven cation ATPases (sll1614, sll1920, slr0671-72, slr0822, slr1507-08-09,...”
- “...of a knock-out fragment for the Na+/H+ antiporter, slr1595 (nhaS4), illustrating how the method works. Specific primers were used to amplify the...”
- Functional analysis of the Na+/H+ antiporter encoding genes of the cyanobacterium Synechocystis PCC 6803
Elanskaya, Biochemistry. Biokhimiia 2002 (PubMed)- “...antiporters encoded by nhaS1 (slr1727), nhaS3 (sll0689), nhaS4 (slr1595), and nhaS5 (slr0415) in salt stress response and internal pH regulation of the...”
- “...type, while the remaining three proteins (Sll0689 = NhaS3; Slr1595 = NhaS4; Slr0415 = NhaS5) are obviously of prokaryotic type (Fig. 1). For two of these...”
- Functional expression in Escherichia coli of low-affinity and high-affinity Na(+)(Li(+))/H(+) antiporters of Synechocystis
Inaba, Journal of bacteriology 2001 - “...et al. [22]), nhaS2 (sll0273), nhaS3 (sll0689), nhaS4 (slr1595), and nhaS5 (slr0415). Bacterial strains and growth conditions. E. coli TO114 (W3110 nhaA::Kmr...”
IGB08_04050 cation:proton antiporter family protein from Lactiplantibacillus plantarum
25% identity, 78% coverage
CHX23_ARATH / Q8VYD4 Cation/H(+) antiporter 23, chloroplastic; Protein CATION/H+ EXCHANGER 23; AtCHX23 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
TC 2.A.37.4.5 / Q8VYD4 Cation/H(+) antiporter 23, chloroplastic (Protein CATION/H+ EXCHANGER 23) (AtCHX23) from Arabidopsis thaliana (see 7 papers)
NP_172049 cation/H+ exchanger 23 from Arabidopsis thaliana
AT1G05580 ATCHX23 (CATION/H+ EXCHANGER 23); monovalent cation:proton antiporter/ sodium:hydrogen antiporter from Arabidopsis thaliana
22% identity, 67% coverage
- function: Operates as a K(+)/H(+) antiporter or Na(+)/H(+) antiporter of the chloroplast envelope that functions in pH homeostasis and chloroplast development. Monovalent cation transporter with a preference for Cs(+), K(+) and Rb(+) relative to Na(+) or Li(+). Required for pollen tube guidance, but not for normal pollen development. May also be involved in the development or function of the female gametophyte.
disruption phenotype: Mutants with impaired chloroplast development that display aberrant chloroplast structure (PubMed:15220473). No defect in male fertility, due to redundancy with CHX21. Chx21 and chx23 double mutants are male sterile (PubMed:21239645). - substrates: H+, cations
- Arabidopsis antiporter CHX23 and auxin transporter PIN8 coordinately regulate pollen growth.
Gao, Journal of plant physiology 2021 (PubMed)- GeneRIF: Arabidopsis antiporter CHX23 and auxin transporter PIN8 coordinately regulate pollen growth.
- Pollen tubes lacking a pair of K+ transporters fail to target ovules in Arabidopsis.
Lu, The Plant cell 2011 - GeneRIF: CHX21 and CHX23, participate in pollen tube guidance and are functionally redundant.
- Identification of Quantitative Trait Locus and Candidate Genes for Drought Tolerance in a Soybean Recombinant Inbred Line Population
Ouyang, International journal of molecular sciences 2022 - “...AT5G19780 TUA5 Glyma.16G041400 putative H/ACA ribonucleoprotein complex subunit 1-like AT3G03920 unknown Glyma.16G039700 cation/H(+) antiporter 15-like AT1G05580 CHX23 Glyma.16G041300 hypothetical protein PRUPE_ppa015420mg AT3G42170 BED zinc finger Glyma.16G036500 hypothetical protein MTR_7g013520 unknown unknown Glyma.16G044300 omega-hydroxy palmitate O-feruloyl transferase-like AT1G65450 unknown Glyma.16G039600 quinate hydroxycinnamoyl transferase-like AT2G19070 SHT Glyma.16G038500 mavicyanin-like...”
- Comparative transcriptome analysis and ChIP-sequencing reveals stage-specific gene expression and regulation profiles associated with pollen wall formation in Brassica rapa
Shen, BMC genomics 2019 - “...11.79 H3K27 AT2G38910 CPK20 calcium dependent protein kinase [ 85 ] Bra015442 6.91 11.81 H3K27 AT1G05580 CHX23 member of putative Na + /H + antiporter family [ 86 ] Bra025052 4.79 14.1 H3K27 AT5G45810 CIPK19 CBL-interacting protein kinase [ 87 ] Bra022172 4.35 15.47 H3K27 AT3G16640...”
- The roles of the cation transporters CHX21 and CHX23 in the development of Arabidopsis thaliana
Evans, Journal of experimental botany 2012 - “...related members of this CHX family. A genotype carrying a knockout of the AtCHX23 gene (At1g05580) showed no phenotype when grown in glasshouse conditions. In particular, it did not exhibit the reduced root growth phenotype observed for a knockout of its homologue AtCHX21 when exposed to...”
- “...from the SAIL collection (Code 899 G8), carrying a putative T-DNA insert in gene AtCHX23 (At1g05580), was obtained from the Nottingham Arabidopsis Stock Centre. Genotypes homozygous for a knockout mutant allele of AtCHX21 (SLAT 01_19_03b) had already been produced (see Hall et al. , 2006 )....”
- Host origin of plastid solute transporters in the first photosynthetic eukaryotes
Tyra, Genome biology 2007 - “...63 distinct genes (lower chart) considered in this study. Table 1 Arabidopsis solute transporters Host At1g05580 Cation/hydrogen exchanger At1g54320 Ligand-effect modulator 3 (LEM3) family At1g59870 ABC transporter At1g61800 Glucose-6-phosphate/phosphate translocator 2 (GPT2) At1g64150 Expressed protein At1g66950 ABC transporter At1g70610 Transporter associated with antigen processing protein 1...”
- A probable Na+(K+)/H+ exchanger on the chloroplast envelope functions in pH homeostasis and chloroplast development in Arabidopsis thaliana
Song, Proceedings of the National Academy of Sciences of the United States of America 2004 - “...analysis. The AtCHX23 gene is located on chromosome I (At1g05580) and encodes a protein of 867-aa residues with a predicted molecular mass of 95.8 kDa. The...”
4gx2B / Q74FS9 Gsuk channel bound to NAD (see paper)
31% identity, 18% coverage
- Ligands: calcium ion; zinc ion; nicotinamide-adenine-dinucleotide (4gx2B)
4gx0D / Q74FS9 Crystal structure of the gsuk l97d mutant (see paper)
32% identity, 19% coverage
- Ligands: calcium ion; zinc ion (4gx0D)
XP_006343907 cation/H(+) antiporter 18-like from Solanum tuberosum
24% identity, 44% coverage
CH_124268 putative potassium:hydrogen antiporter (S.pombe) from Magnaporthe grisea 70-15 (see paper)
24% identity, 40% coverage
CPAA_BACSU / O31615 Putative Na(+)/H(+) antiporter YjbQ from Bacillus subtilis (strain 168) (see paper)
28% identity, 48% coverage
- function: Probable Na(+)/H(+) antiporter.
- A decade of research on the second messenger c-di-AMP
Yin, FEMS microbiology reviews 2020 - “...9 M NA 64.4 3.4 nM ABD21176 CAB13021 ABD22474 A0A0H2XH32 O31615 A0A0H2XI70 BusR TrkH NA NA NA NA NA NA NA B. subtilis S. agalactiae M. pneumoniae B. subtilis...”
sll0993 potassium channel from Synechocystis sp. PCC 6803
P73132 Potassium channel from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
31% identity, 19% coverage
- Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB
Selim, Science advances 2021 - “...potassium transporters in Synechocystis KtrA ( sll0493 ), TrkA ( slr0773 ), and MthK ( sll0993 ). Moreover, the magnesium transporter MgtE ( slr1216 ), the sodium/H + antiporters NhaS2 and NhaS5 ( sll0273 and slr0415 , respectively), and the glutamate-Na + symporter ( slr0625 )...”
- “...K + transporters, including KtrA ( sll0493 ), TrkA ( slr0773 ), and MthK ( sll0993 ) as c-di-AMP targets. This highlights a conserved role for c-di-AMP in controlling osmotic homeostasis and K + transport ( 12 14 ), which is of particular importance, since K...”
- Global Proteomic Analysis Reveals an Exclusive Role of Thylakoid Membranes in Bioenergetics of a Model Cyanobacterium
Liberton, Molecular & cellular proteomics : MCP 2016 - “...protein Hypothetical protein Sll0108 Sll0169 Sll0574 Sll0855 Sll0993 Sll1276 Sll1695 Slr0114 Slr0369 Slr0593 Slr0615 Slr0678 Slr0798 Slr1149 Slr1216 Slr1423...”
- Evidence of SARS-CoV-2-Specific T-Cell-Mediated Myocarditis in a MIS-A Case.
Vannella, Frontiers in immunology 2021 - “...P26312 CASSFSGFTDTQYF 92.86 2 lmmuneCODE P39473 CASSESSGFTDTQYF 93.33 1 lmmuneCODE P73013 CASSYSTFTDTQYF 92.86 2 lmmuneCODE P73132 CASSYSSVTDTQYF 92.86 2 lmmuneCODE P97143 CASSYSGFTDTQYF 100 1 lmmuneCODE P118904 CASSYSSPTDTQYF 92.86 2 lmmuneCODE P120946 CASSYSPGFTDTQYF 93.33 1 lmmuneCODE Clonotype2 CASSRLAGRETQYF NA NA Patient P137116 CASSRLAGREQYF 100 1 lmmuneCODE Clonotype3...”
SAUSA300_0911 transporter, monovalent cation:proton antiporter-2 (CPA2) family protein from Staphylococcus aureus subsp. aureus USA300_FPR3757
SA0868 hypothetical protein from Staphylococcus aureus subsp. aureus N315
NWMN_0880 monovalent cation/H+ antiporter-2 (CPA2) family protein from Staphylococcus aureus subsp. aureus str. Newman
23% identity, 86% coverage
- Molecular mechanisms of thioridazine resistance in Staphylococcus aureus
Wassmann, PloS one 2018 - “...mutants Plasmids for in-frame deletions of relevant genes ( rpoC , SAUSA300_0649 , SAUSA300_0703 , SAUSA300_0911 , pyc , SAUSA300_1119 , SAUSA300_1720 , SAUSA300_1797 , and cls ) were constructed through splicing by overlap extension (SOE) PCR [ 23 ] into the pIMAY plasmid [ 24...”
- “...of the gene was verified by sequencing by Eurofins Genomics for the genes SAUSA300_0649 , SAUSA300_0911 , pyc , SAUSA300_1797 , and cls . The remaining deletion mutants were unsuccessful for different technical reasons. Assessment of membrane potential using flow cytometry Measurement of the membrane potential...”
- Intracellular Concentrations of Borrelia burgdorferi Cyclic Di-AMP Are Not Changed by Altered Expression of the CdaA Synthase
Savage, PloS one 2015 - “...]; Staphylococcus aureus KtrA, GenBank locus SAUSA300_0988 [ 32 ]; Staphylococcus aureus CpaA, GenBank locus SAUSA300_0911 [ 32 ]; Staphylococcus aureus KdpD, GenBank locus AFH70306 [ 32 ]; and Staphylococcus aureus PstA, GenBank locus AFH69624 [ 32 ]. Bacteria and plasmids The cdaA gene was cloned...”
- Systematic identification of conserved bacterial c-di-AMP receptor proteins
Corrigan, Proceedings of the National Academy of Sciences of the United States of America 2013 - “...RCK_C domain. In this manner we discovered the protein SAUSA300_0911 in S. aureus strain LAC*, which we rename CpaA. This protein is a predicted cation/proton...”
- “...the predicted K+ or Na+ antiporter CpaA (SAUSA300_0911), containing an N-terminal transmembrane (yellow) and cytoplasmically located RCK_N (blue) and RCK_C...”
- The C-terminal region of the RNA helicase CshA is required for the interaction with the degradosome and turnover of bulk RNA in the opportunistic pathogen Staphylococcus aureus
Giraud, RNA biology 2015 - “...SA0701 Uncharacterized membrane protein with similarity to diguanylate cyclase DgkC SA0701 3.2 2.9 1.8 3.0 SA0868 SA0863-SA0868 Uncharacterized protein, similar to Putative Na(+)/H(+) antiporter YjbQ [yjbQ] SA0868 2.5 2.4 1.9 2.2 SA0927 SA0927-SA0929 Uncharacterized protein, weakly similar to Putative HMP/thiamine permease protein YkoC SA0927 2.3 2.1...”
- Characterizing the effects of inorganic acid and alkaline shock on the Staphylococcus aureus transcriptome and messenger RNA turnover
Anderson, FEMS immunology and medical microbiology 2010 - “...SA0862 hypothetical protein sa_c7760s6763_at * 19.9 15 15 SA0625 hypothetical protein sa_c8473s7434_a_at 3.0 2.5 5 SA0868 hypothetical protein sa_c10616s11070_s_at 2.1 2.5 2.5 SA0871 hypoyhetical protein sa_c304s144_a_at 4.9 15 15 SA0985 putative surface protein sa_c470s302_at 2.9 2.5 5 SA1026 hypothetical protein sa_c485s314_at 281.2 ND 15 SA1033 hypothetical...”
- [Analysis of protein interaction network and function of Staphylococcus aureus]
Liu, Wei sheng wu xue bao = Acta microbiologica Sinica 2009 (PubMed)- “...was subject to scale-free property and a number of very important proteins, such as SA0939, SA0868, rpID. Through the analysis of the important cell wall synthesis and signal transduction and regulation local networks, we also found some very important proteins. Such information will help us better...”
- Bacillithiol: a key protective thiol in Staphylococcus aureus
Perera, Expert review of anti-infective therapy 2015 - “...( Figure 8 ). The closest B. subtilis KhtU analog in S. aureus Newman is NWMN_0880, a Na + /H + transporter (23% sequence identity with 69% coverage) in the same CPA2 family as the E. coli transporter. Interestingly, glxA shares 2530% sequence identity with S....”
XP_049349907 cation/H(+) antiporter 18-like from Solanum verrucosum
23% identity, 43% coverage
LACBIDRAFT_172283 uncharacterized protein from Laccaria bicolor S238N-H82
24% identity, 41% coverage
- Comparative transcriptomics of the model mushroom Coprinopsis cinerea reveals tissue-specific armories and a conserved circuitry for sexual development
Plaza, BMC genomics 2014 - “...CC1G_06868 SCHCODRAFT_53362 LACBIDRAFT_182606 Endo-1,3(4)-beta-glucanase CC1G_07550 SCHCODRAFT_56140 LACBIDRAFT_291657 Lipase CC1G_05781 SCHCODRAFT_72461 LACBIDRAFT_291413 OrdA protein CC1G_08860 SCHCODRAFT_80181 LACBIDRAFT_172283 Cation/H+exchanger CC1G_09431 SCHCODRAFT_49922 LACBIDRAFT_190903 O-methylsterigmatocystin oxidoreductase CC1G_11000 SCHCODRAFT_107054 LACBIDRAFT_310382 Hypothetical protein 6 (PC: 0.94) CC1G_02440 SCHCODRAFT_103563 LACBIDRAFT_308947 ras GEF CC1G_07544 SCHCODRAFT_80832 LACBIDRAFT_243185 DUF89-containing protein CC1G_05410 SCHCODRAFT_104205 LACBIDRAFT_301153 Hypothetical protein CC1G_02628...”
PH0302 Na(+)/H(+) antiporter from Pyrococcus horikoshii OT3
26% identity, 51% coverage
LOC110699138 LOW QUALITY PROTEIN: cation/H(+) antiporter 20-like from Chenopodium quinoa
25% identity, 48% coverage
- Genome-Wide Transcriptomic and Proteomic Exploration of Molecular Regulations in Quinoa Responses to Ethylene and Salt Stress
Ma, Plants (Basel, Switzerland) 2021 - “...cotransporter (SMT) (LOC110727554), four NRTs (LOC110688100, LOC110684366, LOC110715529, and LOC110684367), one cation/H + antiporter (CAH) (LOC110699138), one Na + /Ca 2+ exchanger (NCL) (LOC110709231), and one aquaporin (LOC110697673) were detected in the quinoa responses to ethylene and salt stress ( Table 2 , Supplementary Material Figure...”
- “...adjustment late response gene LOC110684367 NRT2.4 high-affinity nitrate transporter 2.4 osmotic adjustment late response gene LOC110699138 CAH20 cation/H + antiporter 20 osmotic adjustment late response gene LOC110709231 NCL sodium/calcium exchanger NCL osmotic adjustment late response gene LOC110697673 PIP2-5 aquaporin PIP2-5 osmotic adjustment post-transcriptional modification LOC110737811 OCTN7...”
Q10SB9 K+ efflux antiporter, putative, expressed from Oryza sativa subsp. japonica
31% identity, 28% coverage
CPR_0227 Na/H antiporter (napA) from Clostridium perfringens SM101
24% identity, 49% coverage
CC1G_08860 cation/H+ exchanger from Coprinopsis cinerea okayama7#130
27% identity, 39% coverage
- Comparative transcriptomics of the model mushroom Coprinopsis cinerea reveals tissue-specific armories and a conserved circuitry for sexual development
Plaza, BMC genomics 2014 - “...(PC: 0.99) CC1G_06868 SCHCODRAFT_53362 LACBIDRAFT_182606 Endo-1,3(4)-beta-glucanase CC1G_07550 SCHCODRAFT_56140 LACBIDRAFT_291657 Lipase CC1G_05781 SCHCODRAFT_72461 LACBIDRAFT_291413 OrdA protein CC1G_08860 SCHCODRAFT_80181 LACBIDRAFT_172283 Cation/H+exchanger CC1G_09431 SCHCODRAFT_49922 LACBIDRAFT_190903 O-methylsterigmatocystin oxidoreductase CC1G_11000 SCHCODRAFT_107054 LACBIDRAFT_310382 Hypothetical protein 6 (PC: 0.94) CC1G_02440 SCHCODRAFT_103563 LACBIDRAFT_308947 ras GEF CC1G_07544 SCHCODRAFT_80832 LACBIDRAFT_243185 DUF89-containing protein CC1G_05410 SCHCODRAFT_104205 LACBIDRAFT_301153 Hypothetical...”
HVO_1916 TrkA-N domain family from Haloferax volcanii DS2
24% identity, 81% coverage
MJK1_METJA / Q57604 Potassium channel protein 1; MjK1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
TC 1.A.1.13.8 / Q57604 Potassium channel protein, MjK1 of 333 aas and 6 TMSs. Seems to conduct potassium at low membrane potentials (Hellmer and Zeilinger 2003). Also called TrkA3, a Trk channel with a C-terminal NAD-binding domain from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
25% identity, 27% coverage
- function: Potassium channel protein. Seems to conduct potassium at low membrane potentials.
subunit: Homotetramer. - substrates: K+
B1745_00845 monovalent cation:proton antiporter family protein from Lactobacillus amylolyticus
24% identity, 75% coverage
- Adaptive mechanism of Lactobacillus amylolyticus L6 in soymilk environment based on metabolism of nutrients and related gene-expression profiles
Fei, Food science & nutrition 2022 - “...thioesterase 1.40 B1745_05670 biotin carboxylase 1.58 B1745_00100 mgtC putative Mg 2+ transporter family protein 1.26 B1745_00845 pot potassium transporter 1.36 B1745_05305 amt ammonium transporter 1.97 B1745_06945 cadmiumtranslocating Ptype ATPase 2.16 Genes downregulated Carbohydrate transport and metabolism B1745_06195 hypothetical protein 1.03 B1745_05125 glvB PTS alphaglucoside transporter subunit...”
- “...The expression of five genes, such as mgtC (B1745_00100) coding for Mg 2+ transporter, pot (B1745_00845) coding for potassium transporter, amt (B1745_05305) coding for ammonium transporter, and cadmiumtranslocating Ptype ATPase gene (B1745_06945), was significantly induced in the logarithmic phase, indicating the importance of inorganic ions in...”
SYNW0157 putative Na+/H+ antiporter, CPA2 family from Synechococcus sp. WH 8102
26% identity, 65% coverage
- Computational prediction of the osmoregulation network in Synechococcus sp. WH8102
Mao, BMC genomics 2010 - “...EnvZ SYNW0807 SYNW0807-0808 1 E. coli b3405 OmpR SYNW0808 SYNW0807-0808 0.99 E. coli sll0689 NhaS3 SYNW0157 0.99 PCC6803 sll0493 KtrA SYNW2169 SYNW2165-2170 0.56 PCC6803 slr1509 KtrB SYNW2168 SYNW2165-2170 0.99 PCC6803 slr1508 KtrE SYNW0663 SYNW0663-0667 0.99 PCC6803 b2741 38 SYNW1621 1 E. coli sll0306 RpoD SYNW0102 SYNW0101-0102...”
- “..., we noticed that only the Na + /H + antiporter in PCC6803 maps to SYNW0157 (CPA2 family Na+/H+ antiporter) in WH8102 , while Na + /H + antiporters in E. coli and A.halo do not have any hits. The three K + uptake systems in...”
Npun_F4960 TrkA domain-containing protein from Nostoc punctiforme
27% identity, 19% coverage
- Proteome profiling reveals changes in energy metabolism, transport and antioxidation during drought stress in Nostoc flagelliforme
Li, BMC plant biology 2022 - “...of N. flagelliforme under drought stress. Npun_F5517 is PsbP, Npun_F4810 is PsbO, Npun_R2847 is M16, Npun_F4960 is TrkA, Npun_F3527 is GST, Npun_F2147 is ArsA, htpX is M48, Npun_F6222 is VacB, Npun_F3917 is FBP, Npun_F5056 is S41 and Npun_F3917 is GlpX Physiological observations The contents of soluble...”
Synpcc7942_0307 Na+/H+ antiporter from Synechococcus elongatus PCC 7942
27% identity, 44% coverage
- The Freshwater Cyanobacterium Synechococcus elongatus PCC 7942 Does Not Require an Active External Carbonic Anhydrase
Kupriyanova, Plants (Basel, Switzerland) 2024 - “...Synpcc7942_1469, Synpcc7942_1473, and Synpcc7942_1474); potential Na + /H + antiporters Nha17 (Synpcc7942_0811, Synpcc7942_1264, Synpcc7942_2359, Synpcc7942_0546, Synpcc7942_0307, Synpcc7942_2394, and Synpcc7942_2186); as well as the PxcA proton pump (Synpcc7942_0991). The following genes were selected for this study: (1) ndhD5 (Synpcc7942_1473), which encodes one of the Mnh complexs subunits;...”
MJK2_METJA / Q58752 Probable potassium channel protein 2; MjK2 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
23% identity, 29% coverage
- function: Probable potassium channel protein.
rrnAC2811 potassium channel related protein from Haloarcula marismortui ATCC 43049
25% identity, 45% coverage
- Genome information management and integrated data analysis with HaloLex
Pfeiffer, Archives of microbiology 2008 - “...OE3895F rrnAC2806 381 228 Extended NP1698A, HQ1429A, OE3894R rrnAC2810 909 990 Shortened OE3892R, NP1688A, HQ3137A rrnAC2811 1,905 1,128 Extended OE3889R, HQ3102A, NP1576A rrnAC2818 1,344 1,479 Shortened NP2252A, HQ3104A, OE3882R rrnAC2822 951 309 Extended NP2248A, OE3879F, HQ3106A rrnAC2831 540 411 Extended NP5076A, HQ1339A, OE3871R rrnAC2834 1,032 1,677...”
DVU1606 potassium uptake protein, TrkA family from Desulfovibrio vulgaris Hildenborough
29% identity, 19% coverage
XP_006361543 cation/H(+) antiporter 15-like from Solanum tuberosum
23% identity, 40% coverage
BMQ_1747 Na+/H+ antiporter from Bacillus megaterium QM B1551
25% identity, 53% coverage
MJ1275 NA(+)/H(+) antiporter (napA) from Methanocaldococcus jannaschii DSM 2661
22% identity, 58% coverage
- A bioenergetic basis for membrane divergence in archaea and bacteria
Sojo, PLoS biology 2014 - “...consistent with this prediction. BLAST [47] results show a match for archaeon Methanocaldococcus jannaschii 's Mj1275 SPAP to an equivalent or very closely related protein in at least one member of 35 out of all 37 prokaryotic phyla reported to date ( Table S2 ). The...”
- “...in speed) from http://github.com/UCL/membranedivergence BLAST Searches The primary amino acid sequence of the M. jannaschii Mj1275 Na + /H + antiporter (SPAP) was obtained from the NCBI protein sequence database. Mj1275 is one of three known SPAP genes in archaeon M. jannaschii , the other two...”
MSMEG_6769 transporter, monovalent cation:proton antiporter-2 (CPA2) family protein from Mycobacterium smegmatis str. MC2 155
25% identity, 61% coverage
Q7UEH3 Probable glutathione-regulated potassium-efflux system protein KefB from Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)
37% identity, 11% coverage
XP_036009778 transmembrane and coiled-coil domain-containing protein 3 isoform X1 from Mus musculus
41% identity, 10% coverage
An15g04050 uncharacterized protein from Aspergillus niger
26% identity, 48% coverage
AM1_4901 potassium transporter, VIC family, putative from Acaryochloris marina MBIC11017
24% identity, 19% coverage
- Computational prediction of cAMP receptor protein (CRP) binding sites in cyanobacterial genomes
Xu, BMC genomics 2009 - “...factors MBIC11017 AM1_1272 AM1_1560 AM1_0526 AM1_2193 AM1_2114 AM1_2462 AM1_5490 AM1_0481 AM1_1165 AM1_6038 AM1_0773 AM1_2986 AM1_3534 AM1_4901 AM1_2335 AM1_5208 AM1_5107 AM1_2792 AM1_5169 AM1_3844 AM1_4185 AM1_5140 AM1_0632 ATCC29413 Ava_4451 Ava_3710 Ava_0640 Ava_1491 Ava_4669 Ava_0687 Ava_4995 Ava_1172 Ava_0874 Ava_4457 Ava_0873 Ava_0613 Ava_4753 Ava_1559 Ava_3542 Ava_1149 Ava_3207 Ava_3867 Ava_4503 Ava_3009...”
DR1149 Na+/H+ antiporter, putative from Deinococcus radiodurans R1
28% identity, 56% coverage
A0A0P9D655 Potassium transporter TrkA from Kouleothrix aurantiaca
27% identity, 18% coverage
- New Insights into Plant TPK Ion Channel Evolution
Dabravolski, Plants (Basel, Switzerland) 2021 - “...Archaea VDC-free protein A0A133VJR6 was clustered together with proteins from photosynthetic bacteria Kouleothrix aurantiaca (VDC-free A0A0P9D655) and Anabaena sp. WA102 (VDC-containing A0A0K2LYX4). The second branch contains two VDC proteins: archaeal A0A062V1H8 and bacterial Bilophila wadsworthia (E5YBI1). Similarly, the third branch contains proteins from bacteria and archaea:...”
CCNA_01688 potassium channel protein from Caulobacter crescentus NA1000
26% identity, 26% coverage
DDB_G0292830 hypothetical protein from Dictyostelium discoideum AX4
22% identity, 44% coverage
- The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA
Li, Cell host & microbe 2009 - “...DDB_G0292986 ABC transporters (3 genes) 2.252.85 2.293.17 0.731.10 DDB_G0274661 Phosphotransferase membrane protein 2.21 2.73 1.39 DDB_G0292830 sodium, hydrogen exchanger 6.67 2.16 1.00 DDB_G0283345 Sugar transporter ComD 10.69 2.89 0.77 Development Reduced Expression DDB_G0281823 V4-7,vegetative stage specific 0.34 0.09 0.26 Enhanced Expression DDB_G0273919 Discoidin I-like chains (2...”
BH05860 Potassium uptake protein from Bartonella henselae str. Houston-1
30% identity, 20% coverage
LSA0134 Putative Na(+)/H(+) antiporter from Lactobacillus sakei subsp. sakei 23K
26% identity, 46% coverage
- Global transcriptome response in Lactobacillus sakei during growth on ribose
McLeod, BMC microbiology 2011 - “...and metabolism Transport/binding of inorganic ions LSA0029 lsa0029 Putative ion Mg(2+)/Co(2+) transport protein, hemolysinC-family -0.7 LSA0134 lsa0134 Putative Na(+)/H(+) antiporter -0.6 LSA0180 mtsC Manganese ABC transporter, ATP-binding subunit -0.8 LSA0181 mtsB Manganese ABC transporter, membrane-spanning subunit -0.8 -1.0 LSA0182 mtsA Manganese ABC transporter, substrate-binding lipoprotein precursor...”
XP_006341222 cation/H(+) antiporter 20 from Solanum tuberosum
24% identity, 49% coverage
KHA1 / CAL69581.1 putative sodium KHA1 transporter from Debaryomyces hansenii (see paper)
20% identity, 44% coverage
TM1057 potassium channel, putative from Thermotoga maritima MSB8
30% identity, 18% coverage
- The Thermotoga maritima Trk potassium transporter--from frameshift to function
Johnson, Journal of bacteriology 2009 - “...pMT2765/6 pSpeedET no insert pSpeedET TM1088A, TM1088B TM1057 TM1088A TM1088A, TM1088B, TM1089 TM1088B, TM1089 TM1089 TM1088A G14AG16AG19A, TM1088B, TM1089...”
- “...the membrane-spanning protein of the Trk system; and TM1057, another TrkA-like annotated protein. Aspects of TM1088 are unusual for a putative K transporter....”
Mb3225c POSSIBLE TRANSMEMBRANE CATION TRANSPORTER from Mycobacterium bovis AF2122/97
25% identity, 24% coverage
Rv3200c POSSIBLE TRANSMEMBRANE CATION TRANSPORTER from Mycobacterium tuberculosis H37Rv
25% identity, 24% coverage
- Telacebec Interferes with Virulence Lipid Biosynthesis Protein Expression and Sensitizes to Other Antibiotics
Zhou, Microorganisms 2023 - “...Rv3731 LigC DNA ligase C 0.00194 4.862120018 Others Rv1287 NA HTH-type transcriptional regulator 0.02526 2.906914209 Rv3200c NA Transmembrane cation transporter 0.01681 2.480604421 Rv1072 NA Transmembrane protein 0.0353 1.432802446 Rv1849 UreB Urease subunit beta 0.00076 0.150994385 Rv3011c GatA Glutamyl-tRNA amidotransferase subunit A 0.04319 0.738761056 Rv3290c Lat L-lysine-epsilon...”
- Tuberculosis Severity Predictive Model Using Mtb Variants and Serum Biomarkers in a Colombian Cohort of APTB Patients
Ocampo, Biomedicines 2023 - “...acid stress, and nutrient availability [ 47 ]. Additionally, a polymorphism was found in the Rv3200c protein, a putative potassium channel [ 48 ]. The second region (R2) corresponds to the PPE and PE_PGRS protein family members. Here, we highlight a polymorphism in the pe_pgrs30 gene,...”
- Selective Pressure by Rifampicin Modulates Mutation Rates and Evolutionary Trajectories of Mycobacterial Genomes
Cebrián-Sastre, Microbiology spectrum 2023 - “...homologs of some candidate genes have been detected in rifampicin-resistant isolates of M. tuberculosis : Rv3200c (MSMEI_1903; mchK ), Rv1384 (MSMEI_2973; carB ), Rv2006 (MSMEI_3863; otsB1 ), Rv2343c (MSMEI_4371; dnaG ), Rv3823c (MSMEI_4625; mmpL ), Rv0803 (MSMEI_5666; purL ), and Rv3689 (MSMEI_6055; transmembrane protein gene) (in...”
- Inactivation of a New Potassium Channel Increases Rifampicin Resistance and Induces Collateral Sensitivity to Hydrophilic Antibiotics in Mycobacterium smegmatis
Do, Antibiotics (Basel, Switzerland) 2022 - “...protein also showed an 81% sequence similarity with another putative cation channel from M. tuberculosis (Rv3200c) ( Figure 1 b). The M. smegmatis target gene MSMEG_1945 identified in our screening was denominated mchK (for mycobacterial channel K + ). Gene products were denominated MchK sm and...”
- Whole-Genome Sequencing Analysis of Serially Isolated Multi-Drug and Extensively Drug Resistant Mycobacterium tuberculosis from Thai Patients
Faksri, PloS one 2016 - “...showed missense SNPs at genomic position 3,573,234 G>A (CGC>TGC of the minus strand, Arg146Cys) in Rv3200c (non-essential gene, possible transmembrane cation transporter [ 37 ]) and a reverse missense SNP at genomic position 4,139,678 G>T (AGC>AGA of the minus strand, Ser26Arg) in glpK (non-essential gene predicted...”
- Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: expanding the mycobacterial phosphoproteome catalog
Fortuin, Frontiers in microbiology 2015 - “...* LER T This study Rv3197 Rv3197 S # KDEVTAELMEK S Prisic et al., 2010 Rv3200c Rv3200c QSGADTVVVSS $ ETAGR S Prisic et al., 2010 ; This study QSGADTVVVS * SETAGR S This study Rv3248c SahH GVTEETTTGVLR Not known Prisic et al., 2010 GVTEETT * T...”
- Expression of the Genes Encoding the Trk and Kdp Potassium Transport Systems of Mycobacterium tuberculosis during Growth In Vitro
Cholo, BioMed research international 2015 - “...have been identified, namely, the Trk and Kdp. However, in addition, the genes, mbtG , Rv3200c , and Rv3237c , encode the putative K + channels, L-lysine 6-monooxygenase, a possible transmembrane cation transporter, and a conserved hypothetical protein, respectively [ 6 ]. These have been sequenced...”
- Detecting the molecular scars of evolution in the Mycobacterium tuberculosis complex by analyzing interrupted coding sequences
Deshayes, BMC evolutionary biology 2008 - “...Intermediary metabolism 0070 (Rv3172c 160 aa ) 0090 0069 0083 ICDS Conserved hypothetical Unknown 0071 (Rv3200c 355 aa ) 0091 0101 0122 ICDS Hypothetical Unknown 0075 (Rv3349c 246 aa ) 0100 0102 0085 ICDS Transposase IS/phage 0076 (Rv3351c 264 aa Rv3352c 123 aa ) 0101 0071...”
- More
vnz_04410 cation:proton antiporter from Streptomyces venezuelae
31% identity, 29% coverage
sll0536 hypothetical protein from Synechocystis sp. PCC 6803
30% identity, 16% coverage
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in Cyanobacterium Synechocystis sp. PCC 6803
Lin, International journal of molecular sciences 2023 - “...slr1821 would exaggerate the ammonium toxicity. Secondly, WT decreased the expression of potassium channel ( sll0536 ), cation exporters of nickel and cobalt ( slr0793 ) and an ABC transporter for guanidine ( sll1080-1082 ). It was consistent with the repression of guanidine hydrolase as mentioned...”
- Genome-wide protein-protein interactions and protein function exploration in cyanobacteria
Lv, Scientific reports 2015 - “...sub-network 14. In this sub-network, six function unknown proteins (Slr1660, Slr1658, Slr1657, Sll0537, Slr1659 and Sll0536) were assigned to GO terms: catalytic activity (GO:0003824), cellular ketone metabolic process (GO:0042180), regulation of cellular process (GO:0050794), ligase activity (GO:0016874) and hydrolase activity (GO:0016787). These proteins with unknown function...”
- RNA-seq based identification and mutant validation of gene targets related to ethanol resistance in cyanobacterial Synechocystis sp. PCC 6803
Wang, Biotechnology for biofuels 2012 - “...iron carrying protein 2.31 1.70 sll0450 cytochrome b subunit of nitric oxide reductase 1.62 4.26 sll0536 probable potassium channel protein 2.00 1.93 sll0540 phosphate-binding protein PstS homolog 1.57 1.79 sll0613 Holliday junction DNA helicase RuvB 1.73 1.91 sll0621 putative c-type cytochrome biogenesis protein CcdA 2.22 2.00...”
- “...Nat permease, sll0540 encoding a phosphate-binding protein PstS homolog, sll0671 encoding a probable cation transporter, sll0536 encoding a probable potassium channel protein, sll1428 encoding a probable sodium-dependent transporter, slr2131 encoding a RND multidrug efflux transporter, sll0384 encoding a cation and iron carrying protein, sll1041 encoding a...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
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by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory