PaperBLAST
PaperBLAST Hits for tr|Q9I498|Q9I498_PSEAE Probable enoyl-CoA hydratase/isomerase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA1240 PE=1 SV=1 (265 a.a., MSEANSGPGR...)
Show query sequence
>tr|Q9I498|Q9I498_PSEAE Probable enoyl-CoA hydratase/isomerase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=PA1240 PE=1 SV=1
MSEANSGPGRVTREQRGHLFLIGLDRAGKRNAFDSAMLADLALAMGEYERSEESRCAVLF
AHGEHFTAGLDLMELAPKLAASGFRYPDGGVDPWGVVQPRRSKPLVVAVQGTCWTAGIEL
MLNADIAVAARGTRFAHLEVLRGIPPLGGSTVRFPRAAGWTDAMRYILTGDEFDADEALR
MRLLTEVVEPGEELARALEYAERIARAAPLAVRAALQSAFQGRDEGDDAALSRVNESLAA
LIGSEDVREGVLAMVQKRAPAFKGR
Running BLASTp...
Found 250 similar proteins in the literature:
Q9I498 Probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA1240 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...(P23966, P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at...”
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...gnyBH, accD, desB, fadD1, fabABDF1F2GH2IZ, uppS, mdcA, atuC,PA0098, PA0182,PA0286, PA0493, PA0506-PA0508, PA0745-PA0746, PA0879, PA1020-PA1022, PA1187, PA1240, PA1470, PA1535, PA1576, PA1628-PA1629, PA1631, PA1827, PA1869, PA2550, PA2552, PA2815, PA2841, PA2887-PA2891, PA2893, PA3286, PA3426, PA3589, PA3591, PA3593, PA3924,PA4089, PA4330, PA4912, PA4979-PA4980, PA4995, PA5020, PA5524 Protein and Amino acid metabolism...”
- “...showed that 8 genes encoding putative enoyl-CoA hydratase/isomerase ( PA3591, PA1021, PA1629, PA2890, PA3426, PA4330, PA1240 , and PA2841 ) were highly up-regulated in our study, known PPI data showed that only PA4980 directly interacts with a sensor kinase and its response regulator (Yang and Lu,...”
- Comparative systems biology analysis to study the mode of action of the isothiocyanate compound Iberin on Pseudomonas aeruginosa
Tan, Antimicrobial agents and chemotherapy 2014 - “...PA0201 PA0202 PA0283 PA0284 PA0565 PA0849 PA0865 PA0878 PA1240 PA1260 PA1285 PA1310 PA1332 PA1334 PA1493 PA1999 PA2000 PA2008 PA2009 PA2197 PA2247 PA2248 PA2249...”
ABUW_2957 crotonase/enoyl-CoA hydratase family protein from Acinetobacter baumannii
59% identity, 97% coverage
Q9I002 Probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA2841 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa PAO1
56% identity, 97% coverage
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at least...”
- Evolution of lasR mutants in polymorphic Pseudomonas aeruginosa populations facilitates chronic infection of the lung
Zhao, Nature communications 2023 - “...PA1362 gatC lasR, pscR, pilS tle3, mucA, ptrB, cupB5, yheU, yqaA, PA0190, PA1153, PA1879, PA2315, PA2841, PA2875, PA3470, PA3884 W4a a phzF1, algO, PA0946 PA0946 W6b a phzF1, phzE, glnA W3a 3280 genes opdH, phlE, ymdC gatC pscR yheU, yqaA, PA0190, PA1879, PA3470, PA3884 W5b b...”
- “...ymdC gatC, bkdR pscR, algR tle3, cupB3, rocS1, vfr, yheU, yqaAPA0126, PA0190, PA1153, PA1879, PA2315, PA2841, PA3884 a The genome sequences of the isolates belonging to Subgroup 1 in Fig. 2b were compared to that of W1c. b The genome sequences of the isolates belonging to...”
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...PA0745-PA0746, PA0879, PA1020-PA1022, PA1187, PA1240, PA1470, PA1535, PA1576, PA1628-PA1629, PA1631, PA1827, PA1869, PA2550, PA2552, PA2815, PA2841, PA2887-PA2891, PA2893, PA3286, PA3426, PA3589, PA3591, PA3593, PA3924,PA4089, PA4330, PA4912, PA4979-PA4980, PA4995, PA5020, PA5524 Protein and Amino acid metabolism thrS, folC, glnA, gmk, tgt, dadA, pauA3A5, gltX, gcvT1T2, glyQ, gdhA,...”
- “...genes encoding putative enoyl-CoA hydratase/isomerase ( PA3591, PA1021, PA1629, PA2890, PA3426, PA4330, PA1240 , and PA2841 ) were highly up-regulated in our study, known PPI data showed that only PA4980 directly interacts with a sensor kinase and its response regulator (Yang and Lu, 2007 ). Similarly,...”
MSMEG_3362 enoyl-CoA hydratase from Mycobacterium smegmatis str. MC2 155
46% identity, 96% coverage
ECH19_MYCS2 / A0R4Q3 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
MSMEG_5915 enoyl-CoA hydratase from Mycobacterium smegmatis str. MC2 155
35% identity, 99% coverage
- function: Degradation of the cholesterol side chain involves 3 multistep beta-oxidation cycles, this may be involved in the second cycle. Hydrates 3-OCDO-CoA ((22E)-3-oxo-chol-4,22-dien-24-oyl-CoA) to make 22-HOCO-CoA (22-hydroxy-3-oxo-cholest-4-en-24-oyl-CoA). Not active on (E)-3-OCDS-CoA ((E)-3-oxocholest- 4,24-dien-26-oyl-CoA) or 3-OPDC- CoA (3-oxo-4,17-pregnadiene-20-carboxyl-CoA). Hydrates the same substrate as ChsH3 (AC I7GCU8), but the 2 enzymes make different stereoisomers of the product.
catalytic activity: (22E)-3-oxochola-4,22-dien-24-oyl-CoA + H2O = (22R)-hydroxy-3- oxo-chol-4-ene-24-oyl-CoA (RHEA:72575)
subunit: Homotrimer; substrate probably binds in elongated tunnels between the subunits.
disruption phenotype: Not required for growth on rich medium. Grows slower than wild-type on beta-sitosterol (a C-24 branched side chain sterol), beta-sitosterol degradation is slower in this strain. - Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...class IV Intermediary metabolism and respiration 2.2 MSMEG_6365 Rv3780 conserved hypothetical protein Conserved hypotheticals 2.1 MSMEG_5915 Rv3516 ( echA19 ) enoyl-CoA hydratase Lipid metabolism 1.2 MSMEG_1530 integral membrane protein Conserved hypotheticals 1.1 MSMEG_0408 ( pks ) type I modular polyketide synthase Lipid metabolism 6.0 MSMEG_5866 Rv0761c...”
- Pathogen roid rage: cholesterol utilization by Mycobacterium tuberculosis
Wipperman, Critical reviews in biochemistry and molecular biology 2014 - “...PE_PGRS57 PE family of glycine rich proteins ? Rv3515c MSMEG_5914 fadD19 3-keto-4-cholest-4-ene-26-oyl-CoA ligase KstR1 Rv3516 MSMEG_5915 echA19 enoyl-CoA hydratase ? Induced KstR1 Rv3517 - conserved hypothetical protein ? Rv3518c MSMEG_5918 cyp142 cholest-4-en-3-one 26-monooxygenase Induced KstR1 Rv3519 MSMEG_5919 conserved hypothetical protein ? Essential KstR1 Rv3520c MSMEG_5920 coenzyme...”
- A highly conserved mycobacterial cholesterol catabolic pathway
García-Fernández, Environmental microbiology 2013 - “...ID Name Annotation References MSMEG_5914 Rv3515c 83 FadD19 Acyl-CoA synthetase Cole et al ., 1998 MSMEG_5915 Rv3516 82 EchA19 Enoyl-CoA hydratase Cole et al ., 1998 MSMEG_5916 ---- ---- ---- ---- -- MSMEG_5917 Rv3517 59 ---- Hypothetical protein -- MSMEG_5918 Rv3518c 78 CYP142A1 Cytochrome P450 monooxygenase...”
- A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis
Kendall, Molecular microbiology 2007 - “...MSMEG_2645 cgcagtactgggtcatgaaa gagtggatcggcgagtagaa MSMEG_3519 gtacacccccgaacagctt gtccatgccctcgtacttgt MSMEG_5555 cactgttcgacaaccgtctg tggtgtcatcttggtcttgg MSMEG_5904 cgtcatgcgagttgaagttg caccggatcggatttcac MSMEG_5914 ctcggcactcgagaagagtt aacacgcggtagatgtcctc MSMEG_5915 ccaaggagtacggcctgat tcacggatggtcttgaggat MSMEG_5925 acacctacggcgagttcaag cttccagatctcgacgtcct MSMEG_ 5932 agacctcactcaacggcact gaactgcaggtaacccttgc MSMEG_5995 atcccttccgatttcgactt gcctcgacacctccttgac MSMEG_5996 gccgagacctactaccacctc gttgacgttcaccttgtcca MSMEG_6038 tcgatgagatcggcttcttc cagttgtgcacaccgatgat MSMEG_6039 tccacatcgaggtgttcaag gtccagcagtacatcgagca MSMEG_6041 gcttccggcacagtcgaatt gggtgcgttgtccagctcg MSMEG_6043 cgatttcgacggcacactcgc ggcacgtccggagatcag MSMEG_2758 (sigA) ccaagggctacaagttctcg...”
MT3617 enoyl-CoA hydratase from Mycobacterium tuberculosis CDC1551
34% identity, 98% coverage
- REMap: Operon map of M. tuberculosis based on RNA sequence data
Pelly, Tuberculosis (Edinburgh, Scotland) 2016 - “...MT1376-MT1376.1 MT1373-MT1382 MT1373-MT1382 MT1874-MT1875 MT1874,MT1875-MT1876 MT1868- MT1874,MT1875-MT1877 MT2816-MT2815 MT2816,MT2814-MT2815,MT2817 MT2814-MT2817 MT3240-MT3241 MT3233-MT3246 MT3233-MT3246 MT3617-MT3618 MT3617,MT3618 MT3617, MT3618 expression below cutoff Operons in bold indicate operons that are predicted identically between 2 programs. Commas separate independently transcribed units. Table 3 Summary of operons identified by REMap for...”
ECH19_MYCTU / O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 5 papers)
33% identity, 100% coverage
- function: Degradation of the cholesterol side chain involves 3 multistep beta-oxidation cycles, this may be involved in the second cycle (Probable). Hydrates 3-OCDO-CoA ((22E)-3-oxo-chol-4,22-dien-24- oyl-CoA) to make (22R)-HOCO-CoA (3-oxo-chol-4-ene-(22R)-hydroxy-24-oyl- CoA). Also acts on octenoyl-CoA. Not active on (E)-3-OCDS-CoA ((E)-3- oxocholest-4,24-dien-26-oyl-CoA) or 3-OPDC-CoA (3-oxo-4,17-pregnadiene- 20-carboxyl-CoA). Hydrates the same substrate as ChsH3, but the 2 enzymes make different stereoisomers of the product (PubMed:32649175, PubMed:33826843).
catalytic activity: (22E)-3-oxochola-4,22-dien-24-oyl-CoA + H2O = (22R)-hydroxy-3- oxo-chol-4-ene-24-oyl-CoA (RHEA:72575)
subunit: Homotrimer; substrate probably binds in elongated tunnels between the subunits.
disruption phenotype: Not required for growth in a mouse tuberculosis model (PubMed:14569030). Not required for growth on cholesterol (PubMed:21980284). - Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress
Lu, Microbiology spectrum 2023 - “...), PSase PhyA ( P9WHP3 , Rv3397c ), cholesterol catabolism related enoyl-CoA hydratase EchA19 ( O53561 , Rv3516 ), gathered together and the SOH modification levels of these proteins were all increased. Among them, FadA, was formerly reported as a hypoxia-induced mycobacterial protein suppressing host immunity...”
- Enzymatic β-Oxidation of the Cholesterol Side Chain in Mycobacterium tuberculosis Bifurcates Stereospecifically at Hydration of 3-Oxo-cholest-4,22-dien-24-oyl-CoA
Yuan, ACS infectious diseases 2021 - “...7LG9 and 7LGB. UniProt accession IDs for proteins used in this work: ChsH3, Q6MWW2; EchA19, O53561; ChsB1, O53547. Author Present Address UCB Canada Inc. 2201 Bristol Circle, Suite 602, Oakville, Ontario, L6H 0J8 Author Contributions T.Y., J.M.W., and X.Y.Y. contributed equally to this work. The authors...”
- Post-translational Succinylation of Mycobacterium tuberculosis Enoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA
Bonds, ACS infectious diseases 2020 - “...ACS Publications website. Accession codes UniProt accession IDs for proteins used in this work: EchA19, O53561; MCR, O06543; Rv1151c, P9WGG3; ChsE4-ChsE5, I6YCA3-I6Y3Q0; FadD17, O53551. The structure factors and atomic coordinates for Mtb EchA19 have been deposited into the Protein Data Bank with accession code 6WYI. Authors...”
KR76_14215 crotonase/enoyl-CoA hydratase family protein from Pimelobacter simplex
35% identity, 93% coverage
Rv3516 enoyl-CoA hydratase from Mycobacterium tuberculosis H37Rv
RVBD_3516 crotonase/enoyl-CoA hydratase family protein from Mycobacterium tuberculosis H37Rv
33% identity, 98% coverage
- Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress
Lu, Microbiology spectrum 2023 - “...FadA ( Rv0859 ), EchA7 ( Rv0971c ), EchA16 ( Rv2831 ), and EchA19 ( Rv3516 ) were all increased, which were involved in fatty acid metabolism, valine, leucine and isoleucine degradation, lysine degradation, fatty acid degradation, tryptophan metabolism, benzoate degradation, butanoate metabolism pathways, etc. The...”
- “...PhyA ( P9WHP3 , Rv3397c ), cholesterol catabolism related enoyl-CoA hydratase EchA19 ( O53561 , Rv3516 ), gathered together and the SOH modification levels of these proteins were all increased. Among them, FadA, was formerly reported as a hypoxia-induced mycobacterial protein suppressing host immunity via modulation...”
- Enzymatic β-Oxidation of the Cholesterol Side Chain in Mycobacterium Tuberculosis Bifurcates Stereospecifically at Hydration of 3-Oxo-Cholest-4,22- Dien-24-Oyl-CoA
Yuan, 2021 - A Comprehensive Map of Mycobacterium tuberculosis Complex Regions of Difference
Bespiatykh, mSphere 2021 - “...this locus [data not shown]). The RD316 (1,297bp) deletion, resulting in the loss of the Rv3516 and Rv3517 genes, is specific to all members of lineage 3. Rv1179c and was truncated by RD306 (256bp), which was specific to lineage 4.4.1.1 and lineage 4.4.1.2 sublineages. FIG3 RD...”
- Enzymatic β-Oxidation of the Cholesterol Side Chain in Mycobacterium tuberculosis Bifurcates Stereospecifically at Hydration of 3-Oxo-cholest-4,22-dien-24-oyl-CoA
Yuan, ACS infectious diseases 2021 - “...ChsH3 favors the 22 S hydration of 3-oxo-cholest-4,22-dien-24-oyl-CoA in contrast to the previously reported EchA19 (Rv3516), which catalyzes formation of the (22 R )-hydroxy-3-oxo-cholest-4-en-24-oyl-CoA from the same enoyl-CoA substrate. ChsB1 is stereospecific and catalyzes dehydrogenation of the ChsH3 product but not the EchA19 product. The X-ray...”
- Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC
Karlikowska, Cell surface (Amsterdam, Netherlands) 2021 - “...IV Intermediary metabolism and respiration 2.2 MSMEG_6365 Rv3780 conserved hypothetical protein Conserved hypotheticals 2.1 MSMEG_5915 Rv3516 ( echA19 ) enoyl-CoA hydratase Lipid metabolism 1.2 MSMEG_1530 integral membrane protein Conserved hypotheticals 1.1 MSMEG_0408 ( pks ) type I modular polyketide synthase Lipid metabolism 6.0 MSMEG_5866 Rv0761c (...”
- Ancient Bacterial Class Alphaproteobacteria Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species
Nzuza, International journal of molecular sciences 2021 - “...48 Acyl-CoA dehydrogenase echA9 Rv1071c 3-Hydroxyisobutyryl-CoA hydrolase pzu:PHZ_c1679 39 57 Enoyl-CoA hydratase/isomerase family protein echA19 Rv3516 Possible enoyl-CoA hydratase pzu:PHZ_c2535 38 54 Enoyl-CoA hydratase/carnithine racemase echA20 Rv3550 Possible enoyl-CoA hydratase pzu:PHZ_c3429 33 51 Enoyl-CoA hydratase/isomerase family protein fadB2 Rv0468 hydroxybutyryl-CoA dehydrogenase pzu:PHZ_c3152 43 60 3-Hydroxyacyl-CoA dehydrogenase...”
- Post-translational Succinylation of Mycobacterium tuberculosis Enoyl-CoA Hydratase EchA19 Slows Catalytic Hydration of Cholesterol Catabolite 3-Oxo-chol-4,22-diene-24-oyl-CoA
Bonds, ACS infectious diseases 2020 - “...which cholesterol is degraded may permit identification of new therapeutic targets. Here, we characterized EchA19 (Rv3516), an enoyl-CoA hydratase involved in cholesterol side chain catabolism. Steady-state kinetics assays demonstrated that EchA19 preferentially hydrates cholesterol enoyl-CoA metabolite 3-oxo-chol-4,22-diene-24-oyl-CoA, an intermediate of side chain -oxidation. In addition, succinyl-CoA,...”
- “...used as a template for PCR amplification of ChsE4-ChsE5 ( Rv3504-Rv3505 ), MCR (Rv1143), EchA19 (Rv3516), FadD17 ( Rv3506 ), and Rv1151c ( Rv1151c ). The constructs used in this work are listed in Table S1 with the primers listed in Table S2 for either traditional...”
- System OMICs analysis of Mycobacterium tuberculosis Beijing B0/W148 cluster
Bespyatykh, Scientific reports 2019 - “...The high representation of cellular lipid metabolic process (GO:0044255) (Rv0632c, Rv0971c, Rv1142c, Rv1472, Rv3039c, Rv3373, Rv3516) in RUS_B0 has been confirmed as compared to H37Rv 18 . Figure 5 DosR regulon expression and protein level. Bars indicate fold changes in gene expression (blue bars) and protein...”
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- Host-pathogen genetic interactions underlie tuberculosis susceptibility in genetically diverse mice
Smith, eLife 2022 - “...97.08 97.79 Hip31 ansA RVBD_1538 c mod11 8.28 3.32E-02 13 96.82 97.79 99.09 Hip32 echA19 RVBD_3516 mod20 9.68 3.95E-02 13 113.20 114.59 117.64 Hip33 rv1836c RVBD_1836 c mod15 9.19 1.75E-02 14 74.94 76.40 76.43 Hip34 rv2183c RVBD_2183 c mod11 7.75 4.84E-02 16 12.18 14.06 17.92 Hip35...”
YdbR / b1393 putative 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Escherichia coli K-12 substr. MG1655 (see 7 papers)
PAAF_ECOLI / P76082 2,3-dehydroadipyl-CoA hydratase; Enoyl-CoA hydratase; EC 4.2.1.17 from Escherichia coli (strain K12) (see 4 papers)
P76082 enoyl-CoA hydratase (EC 4.2.1.17) from Escherichia coli (see paper)
NP_415911 putative 2,3-dehydroadipyl-CoA hydratase from Escherichia coli str. K-12 substr. MG1655
b1393 enoyl-CoA hydratase-isomerase from Escherichia coli str. K-12 substr. MG1655
35% identity, 96% coverage
- function: Catalyzes the reversible conversion of enzymatically produced 2,3-dehydroadipyl-CoA into 3-hydroxyadipyl-CoA.
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (RHEA:20724)
disruption phenotype: Mutants accumulate delta3-dehydroadipate and are unable to use phenylacetate as a carbon source. - Genetic dissection of the degradation pathways for the mycotoxin fusaric acid in Burkholderia ambifaria T16
Vinacour, Applied and environmental microbiology 2023 (secret) - On the Enigma of Glutathione-Dependent Styrene Degradation in Gordonia rubripertincta CWB2
Heine, Applied and environmental microbiology 2018 - “...Accession no. BAL04135 Q9L9C1 P76081 P76080 P76079 P76078 P76077 P77467 P76083 P76082 P0C7L2 A0R4Z6 P77455 P43491 P76084 % ID 76 68 43 42 42 67 66 37 36 36 55...”
- Catabolism of the Last Two Steroid Rings in Mycobacterium tuberculosis and Other Bacteria.
Crowe, mBio 2017 - “...1330 12450 e Short-chain-type dehydrogenase/reductase A6CQL2 34 echA20 Rv3550 RS27700 6001 1280 00335 HIEC-CoA hydrolase P76082 (1,4-dihydroxy-2-naphthoyl-CoA synthase [MenB]) 28 ipdA Rv3551 RS22695 6002 1276 00310 COCHEA-CoA hydrolase, subunit Q59111 (glutaconate CoA-transferase, subunit) 26 ipdB Rv3552 RS22690 6003 1277 00315 COCHEA-CoA hydrolase, subunit Q59111 (glutaconate CoA-transferase,...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q8ZR85, Q8ZL15, P40811, Q9L6T2, Q93IM7), Crotonase family (P23966, P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were...”
- Protein-protein interactions in the β-oxidation part of the phenylacetate utilization pathway: crystal structure of the PaaF-PaaG hydratase-isomerase complex.
Grishin, The Journal of biological chemistry 2012 - GeneRIF: Data indicate that the PaaFG complex has a four-layered structure composed of homotrimeric discs of PaaF and PaaG.
- Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid
Shin, Microbial cell factories 2021 - “...engineered C. glutamicum For trans -2-hexenedioic acid biosynthesis in C. glutamicum , PaaF (KEGG ID: b1393) from E. coli [ 50 ] was added to pZ8-paaH-tesB-paaJ. The resulting vector was named pZ8-paaH-tesB-paaJF and transformed into C. glutamicum . PaaF from E . coli catalyzes the dehydration...”
- Tracing the phylogenetic history of the Crl regulon through the Bacteria and Archaea genomes
Santos-Zavaleta, BMC genomics 2019 - “...CRP (+), IHF (+), PaaX () + MSI [ 10 ] phenylacetate catabolic process paaD b1393 paaAB C D E F G H IJ K CRP (+), IHF (+), PaaX () + MSI [ 10 ] phenylacetate catabolic process paaF b1395 paaAB C D E F...”
- FLIM-MAP: Gene Context Based Identification of Functional Modules in Bacterial Metabolic Pathways
Bhatt, Frontiers in microbiology 2018 - “...metabolic pathways like Butanoate metabolism and Fatty acid metabolism. Therefore, the two genes (b1395 and b1393, respectively, in E. coli K12 MG1655) are also included as part of the above mentioned pathways in homology based methods (KEGG, MetaCyc etc.). KEGG maps b1393 and b1395 to Butanoate...”
- “...(KEGG, MetaCyc) annotate the pathway incorrectly in such cases. On the contrary, since the genes (b1393 and b1395) would only lie in within paa gene cluster, FLIM-MAP would lead to an appropriate assignment of these genes exclusively to the Phenylacetate to Succinyl-CoA Metabolism where they actually...”
- Genomic content typifying a prevalent clade of bovine mastitis-associated Escherichia coli
Goldstone, Scientific reports 2016 - “...activator for tynA and feaB 100 82.1 2 b1385 feaB phenylacetaldehyde dehydrogenase 100 82.2 2 b1393 paaF 2,3-dehydroadipyl-CoA hydratase 100 79.2 2 b1394 paaG 1,2-epoxyphenylacetyl-CoA isomerase, oxepin-CoA-forming 100 76.7 2 b1395 paaH 3-hydroxyadipyl-CoA dehydrogenase, NAD+-dependent 98.5 76.0 2 b1396 paaI hydroxyphenylacetyl-CoA thioesterase 98.5 76.6 2 b1397...”
- 18th Congress of the European Hematology Association, Stockholm, Sweden, June 13–16, 2013
, Haematologica 2013 - Identification and mapping of self-assembling protein domains encoded by the Escherichia coli K-12 genome by use of lambda repressor fusions
Mariño-Ramírez, Journal of bacteriology 2004 - “...b4119 b3929 b3939 b0349 b0783 b4351 b0091 b2733 b2286 b1393 b1396 b0134 b3019 b2564 b4100 b1020 b3724 b0951 b4201 b0467 b0386 b0333 b1304 b1303 b3763 b3947...”
crt / P52046 crotonase monomer (EC 4.2.1.74; EC 4.2.1.150) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) (see 3 papers)
CRT_CLOAB / P52046 Short-chain-enoyl-CoA hydratase; 3-hydroxybutyryl-CoA dehydratase; Crotonase; EC 4.2.1.150 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W) (see 2 papers)
P52046 short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Clostridium acetobutylicum (see paper)
crt 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 from Clostridium acetobutylicum (see paper)
CAC2712 Crotonase (3-hydroxybutyryl-COA dehydratase) from Clostridium acetobutylicum ATCC 824
CA_C2712 short-chain-enoyl-CoA hydratase from Clostridium acetobutylicum ATCC 824
32% identity, 97% coverage
- function: Catalyzes the reversible hydration of crotonyl-CoA. Can also use hexenoyl-CoA but not higher analogs.
catalytic activity: a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain (2E)- enoyl-CoA + H2O (RHEA:52664)
subunit: Homotetramer. - Transcriptional analysis of micronutrient zinc-associated response for enhanced carbohydrate utilization and earlier solventogenesis in Clostridium acetobutylicum
Wu, Scientific reports 2015 - “...In terms of the expression of the pyruvate to butyryl-CoA formation genes, thlA (CAC2873), crt (CAC2712), etfA (CAC2709), etfB (CAC2710), and bcd (CAC2711) were differentially upregulated by no more than 1.75-fold. Surprisingly, thlB (CAC0078) encoding acetyl-CoA acetyltransferase was 0.65-fold downregulated compared to 1.46-fold upregulation of the...”
- Redox-responsive repressor Rex modulates alcohol production and oxidative stress tolerance in Clostridium acetobutylicum
Zhang, Journal of bacteriology 2014 - “...nadC asrT asrA asrB asrC CAP0035 CAC0267 CAC2873 CAC2712 CAC2711 CAC2710 CAC2709 CAC2708 CAC3076 CAC3075 CAC1025 CAC1024 CAC1023 CAC1512 CAC1513 CAC1514 CAC1515...”
- Meta-analysis and functional validation of nutritional requirements of solventogenic Clostridia growing under butanol stress conditions and coutilization of D-glucose and D-xylose
Heluane, Applied and environmental microbiology 2011 - “...CAC2235 CAC2388 CAC2389 CAC2390 CAC2634 CAC2708 CAC2711 CAC2712 CAC3164 CAC3170 CAC3348 CAC3462 CAC3596 CAC3680 CAC3681 0.40506 1.51803 1.07553 1.7949 2.70695...”
- A proteomic and transcriptional view of acidogenic and solventogenic steady-state cells of Clostridium acetobutylicum in a chemostat culture
Janssen, Applied microbiology and biotechnology 2010 - “...2.4 3.0 1.6 C CAC2711 bcd Butyryl-CoA dehydrogenase 2.0 1.8 6.1 2.4 3.1 2.0 I CAC2712 crt Enoyl-CoA hydratase 2.1 1.8 7.2 2.9 3.5 2.5 I CAC2810 Glucoamylase family protein 2.7 2.2 10.1 4.9 5.0 3.6 G CAC2873 thlA Acetyl-CoA acetyltransferase 2.3 1.7 8.1 3.5 3.9...”
- “...of acetyl-CoA to butyryl-CoA, e.g., thiolase A ( thlA , CAC2873), crotonase ( crt , CAC2712), butyryl-CoA dehydrogenase ( bcd , CAC2711), and the subunit of the electron transfer flavoprotein ( etfA , CAC2709). Otherwise, 3-hydroxybutyryl-CoA dehydrogenase ( hbd , CAC2708) and the second subunit of...”
- The role of PerR in O2-affected gene expression of Clostridium acetobutylicum
Hillmann, Journal of bacteriology 2009 - “...CAC2458 CAC2459 CAC2499 CAC2708 CAC2709 CAC2710 CAC2711 CAC2712 CAC2873 CAC3075 CAC3076 CAC3657 CAC3658 CAC3659 Arginine biosynthesis CAC0316 CAC0376 CAC0973...”
- “...CAC0711 to CAC0713, CAC2458 and CAC2459, CAC2708 to CAC2712, CAC3075 and CAC3076, CAC3657 to CAC3659, CAC0973 and CAC0974, and CAC2388 to CAC2391 are genes...”
- Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum
Alsaker, Journal of bacteriology 2005 - “...formation genes thl (CAC2873), hbd (CAC2708), crt (CAC2712), etfA (CAC2709), etfB (CAC2710), and bcd (CAC2711) generally increased in stationary phase but...”
- Transcriptional analysis of spo0A overexpression in Clostridium acetobutylicum and its effect on the cell's response to butanol stress
Alsaker, Journal of bacteriology 2004 - “...stress are related to butyrate formation: crt (CAC2712, encoding crotonase) and ptb (CAC3076, encoding phosphate butyryltrans- ferase). A sugar symporter...”
- Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program
Tomas, Applied and environmental microbiology 2003 - “...in this cluster, including hexokinase (CAC2613) and crotonase (CAC2712). ftsA and ftsZ, which form a predicted bicistronic operon (CAC1692 and CAC1693) (39),...”
- An optimized reverse β-oxidation pathway to produce selected medium-chain fatty acids in Saccharomyces cerevisiae
Garces, Biotechnology for biofuels and bioproducts 2023 - “...HBD ( C. acetobutylicum ) (UniProt ID: P52041), CRT ( C. acetobutylicum ) (UniProt ID: P52046) and TER ( T. denticola ) (UniProt ID: Q73Q47) were amplified by PCR from pVS6, TER ( E. gracilis ) (UniProt ID: Q5EU90) was PCR amplified from pVS1. All the...”
- SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron.
Wu, iScience 2023 - “...database (version 2020.10). Furthermore, protein sequence FASTA from nucleoprotein (SwissProt: P0DTC9) and short-chain-enoyl-CoA hydratase (SwissProt: P52046) were added to the protein database. The spectra search criteria include 10ppm mass tolerance for precursor, 0.02Da for product ions, and trypsin enzyme specificity with up to 2 missed cleavages....”
- Cloning and characterization of the ferulic acid catabolic genes of Sphingomonas paucimobilis SYK-6
Masai, Applied and environmental microbiology 2002 - “...dehydratase of Clostridium acetobutylicum ATCC824 (P52046); MenB_Ecoli, naphthoate synthase of E. coli (P27290); MenB_Hinfl, naphthoate synthase...”
- Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins
Liu, Biotechnology for biofuels 2018 - “...factor I protein Hfq 12 CA_C3125 299 7908 7 3 5.4 Ribosomal protein L29 13 CA_C2712 571 28,400 28 10 4.9 Crotonase 14 CA_C1807 182 10,251 7 5 4.8 Ribosomal Protein S15 15 CA_C3211 1113 10,341 59 6 4.7 DNA binding protein HU 16 CA_P0164 1098...”
- Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
Loder, Applied and environmental microbiology 2015 - “...Ter AdhE AdhE Bad Bdh CA_C2873 CA_C2708 CA_C2712 TDE_0597 CA_P0035 CA_P0035 Cbei_3832 CA_C3298 TTE0549 TTE0548 TTE0544 STHERM_c16300 Cthe_0423 Teth514_0627...”
- Fermentation of oxidized hexose derivatives by Clostridium acetobutylicum
Servinsky, Microbial cell factories 2014 - “...phosphotransacetylase, CA_C1742; 16) acetate kinase, CA_C1743; 17) hydroxybutyryl-CoA dehydrogenase, CA_C2009, CA_C2708; 18) crotonase, CA_C2012, CA_C2016, CA_C2712; 19) butyryl-CoA dehydrogenase, CA_C2711; 20) phosphotransbutyrylase, CA_C3076; 21) butyrate kinase, CA_C1660, CA_C3075; 22) galacturonate symporter, CA_C0694; 23) galacturonate isomerase, CA_C0692 24) altronate oxidoreductase, CA_C0695; 25) altronate dehydratase, CA_C0696; 26) 2-keto-3-deoxygluconokinase,...”
- Converting carbon dioxide to butyrate with an engineered strain of Clostridium ljungdahlii
Ueki, mBio 2014 - “...were amplified by PCR. The amplified genes were thl (NCBI GenBank; CA_C2873), crt (NCBI GenBank; CA_C2712), bcd (NCBI GenBank; CA_C2711), etfB (NCBI GenBank, CA_C2710), etfA (NCBI GenBank; CA_C2709), hbd (NCBI GenBank; CA_C2708), buk (NCBI GenBank; CA_C3075), and ptb (NCBI GenBank; CA_C3076). Primers used for the PCR...”
- Rapid construction of metabolic models for a family of Cyanobacteria using a multiple source annotation workflow
Mueller, BMC systems biology 2013 - “...acetobutylicum and the adhA gene in Synechocystis 6803. EC-gene relationships: 2.3.1.9: CA_C2873, 1.1.1.36: CA_C2708, 4.2.1.17: CA_C2712, 1.3.99.2: CA_C2711, 1.2.1.10: CA_P0035, 1.1.1.-: slr1192. The proposed workflow also served to complete unfinished pathways from i Cyt773. All five models are capable of converting galactose-1-phosphate to fructose-6-phosphate as in...”
G8E09_11740 enoyl-CoA hydratase from Acinetobacter pittii
30% identity, 92% coverage
- Phenotypic Variation and Carbapenem Resistance Potential in OXA-499-Producing Acinetobacter pittii
Zhang, Frontiers in microbiology 2020 - “...1.76 0.0000 0.0024 G8E09_14055 Hypothetical protein 1.76 0.0002 0.0089 G8E09_06975 Hypothetical protein 1.76 0.0000 0.0014 G8E09_11740 Enoyl-CoA hydratase 1.76 0.0003 0.0124 G8E09_12805 LysE family transporter 1.75 0.0000 0.0004 G8E09_08700 3-oxoacid CoA-transferase subunit A 1.74 0.0001 0.0044 G8E09_11745 SDR family oxidoreductase 1.74 0.0012 0.0318 G8E09_12925 OprD family...”
FQU82_01642 enoyl-CoA hydratase from Acinetobacter baumannii
30% identity, 92% coverage
- Transcriptomic analysis reveals the regulatory role of quorum sensing in the Acinetobacter baumannii ATCC 19606 via RNA-seq
Xiong, BMC microbiology 2022 - “...the abaI strain, the upregulated DEGs involved in this pathway included the FQU82_00157, FQU82_00183, FQU82_02324, FQU82_01642( paaF ), FQU82_02187, FQU82_01589( paaK ), FQU82_01581( paaB ), FQU82_01580( paaA ), and FQU82_01583( paaD ), while FQU82_02901 was found to be downregulated. Among the DEGs involved in the arginine...”
- “...10 upregulated DEGs were found to be enriched in the propanoate metabolism: FQU82_00191, FQU82_00562, FQU82_03635, FQU82_01642 ( paaF ), FQU82_00192, FQU82_00189 ( mmsA ), FQU82_00193, FQU82_00159 ( prpB ), FQU82_00160 ( prpC ), and FQU82_00161 ( acnD ). In purine metabolism, 8 DEGs were downregulated, including...”
H16_A1719 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
31% identity, 95% coverage
AzCIB_1939 enoyl-CoA hydratase from Azoarcus sp. CIB
35% identity, 92% coverage
F1721_32640 crotonase/enoyl-CoA hydratase family protein from Saccharopolyspora hirsuta
36% identity, 94% coverage
KPHS_23740 enoyl-CoA hydratase-isomerase from Klebsiella pneumoniae subsp. pneumoniae HS11286
34% identity, 95% coverage
- Use of a combined antibacterial synergy approach and the ANNOgesic tool to identify novel targets within the gene networks of multidrug-resistant Klebsiella pneumoniae
Lee, mSystems 2024 - “...Lysine degradation. Upregulation of KPHS_13090 (lysine decarboxylase 1) and downregulation of KPHS_09840 (glutarate 2-hydroxylase) and KPHS_23740 (enoyl-CoA hydratase-isomerase) were significantly changed after combination treatment exposure. ( H ) Starch and sucrose metabolism. KPHS_04990 (trehalose(maltose)-specific PTS system component IIBC) was upregulated and KPHS_52250 (putative PTS, EIIC) was...”
- “...( G ) KPHS_23680 (enoyl-CoA hydratase), which is involved in phenylalanine metabolism, is linked to KPHS_23740. Downregulation of KPHS_46340 (putrescine aminotransferases) and KPHS_43350 (acetyl-CoA acetyltransferase) results in a connection with the target gene KPHS_28070, which is a novel noncoding sRNA. KPHS_02470 (glucose-6-phosphate isomerase) is a positively...”
BB0763 enoyl CoA dehydratase/isomerase from Bordetella bronchiseptica RB50
34% identity, 93% coverage
paaF / Q845K2 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Pseudomonas sp. Y2 (see paper)
36% identity, 92% coverage
AUO97_RS14195 enoyl-CoA hydratase-related protein from Acinetobacter baumannii
33% identity, 93% coverage
- Mutation in the two-component regulator BaeSR mediates cefiderocol resistance and enhances virulence in Acinetobacter baumannii
Liu, mSystems 2023 - “...<0.0001 AUO97_RS14185 Phenylacetate-CoA oxygenase subunit PaaJ 2.213 <0.0001 AUO97_RS14190 Phenylacetate-CoA oxygenase/reductase subunit PaaK 1.891 <0.0001 AUO97_RS14195 Enoyl-CoA hydratase 1.944 <0.0001 AUO97_RS14200 2-(1,2-epoxy-1,2-dihydrophenyl) acetyl-CoA isomerase 1.607 <0.0001 AUO97_RS14205 3-hydroxyacyl-CoA dehydrogenase 1.478 <0.0001 AUO97_RS19215 CsuA/B 2.057 0.0014 ATCC17978 BaeR S104N AUO97_RS10785 MFS transporter 1.049 <0.0001 AUO97_RS19200 Molecular chaperone...”
- The abaI/abaR Quorum Sensing System Effects on Pathogenicity in Acinetobacter baumannii
Sun, Frontiers in microbiology 2021 - “...( paaB ), AUO97_RS14180 ( paaC ), AUO97_RS14185 ( paaD ), AUO97_RS14190 ( paaE ), AUO97_RS14195 ( paaF ), and AUO97_RS14215 ( paaK1 ) were highly expressed ( Figure 8A ), and the expression of this operon was not changed in the abaIR mutant. Branched-chain amino...”
- “..., the abaIR mutant enhances slightly more virulent than abaI . In the abaI mutant, AUO97_RS14195, AUO97_RS16430, AUO97_RS06660, AUO97_RS18630, and AUO97_RS12705 involved in the propanoate metabolism pathway were upregulated. In the abaIR mutant, AUO97_RS14380, AUO97_RS14375, and AUO97_RS14370 involved in the propanoate metabolism pathway were upregulated. Lipids...”
SIDH_ASPFU / Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see 6 papers)
AFUA_3G03410, Afu3g03410, XP_748661 enoyl-CoA hydratase/isomerase family protein from Aspergillus fumigatus Af293
33% identity, 91% coverage
- function: Mevalonyl-coenzyme A hydratase; part of the siderophore biosynthetic pathway (PubMed:22106303). Aspergillus fumigatus produces 4 types of siderophores, low-molecular-mass iron chelators, including excreted fusarinine C (FsC) and triacetylfusarinine C (TAFC) for iron uptake and intacellular ferricrocin (FC) for hyphal and hydroxyferricrocin (HFC) for conidial iron distribution and storage. TAFC consists of 3 N(2)-acetyl-N(5)-anhydromevalonyl-N(5)- hydroxyornithine residues cyclically linked by ester bonds; FC is a cyclic hexapeptide with the structure Gly-Ser-Gly-(N(5)-acetyl-N(5)- hydroxyornithine)x3. The biosynthesis of all four siderophores depends on the hydroxylation of ornithine, catalyzed by the monooxygenase sidA (PubMed:15504822, PubMed:16113265). Subsequently, the pathways for biosynthesis of extra- and intracellular siderophores split (PubMed:17845073). For biosynthesis of extracellular siderophores, the transacylase sidF transfers anhydromevalonyl to N(5)-hydroxyornithine (PubMed:17845073). The required anhydromevalonyl-CoA moiety is derived from mevalonate by CoA ligation and dehydration catalyzed by sidI and sidH respectively (PubMed:22106303). The acetylation of N(5)- hydroxyornithine for FC biosynthesis involves the constitutively expressed sidL (PubMed:21622789). FC is hydroxylated to HFC by an as yet uncharacterized enzyme during conidiation (PubMed:17845073). Assembly of fusarinine C (FsC) and FC is catalyzed by two different nonribosomal peptide synthetases (NRPS), sidD and sidC respectively (PubMed:17845073). Subsequently, sidG catalyzes N2-acetylation of FsC for forming TAFC (PubMed:17845073). Both extra- and intracellular siderophores are crucial for growth during iron limitation and virulence (PubMed:16113265).
disruption phenotype: Blocks TAFC biosynthesis, decreases resistance to oxidative stress, and attenuates virulence in a murine model of invasive pulmonary aspergillosis (PubMed:22106303). - The Siderophore Ferricrocin Mediates Iron Acquisition in Aspergillus fumigatus
Happacher, Microbiology spectrum 2023 - “...h after initiation of germination before they significantly increased ( Fig.2A ). sidF (AFUA_3G03400), sidH (AFUA_3G03410), sidI (AFUA_1G17190), and mirD (AFUA_3G03440), which are involved in biosynthesis and uptake of fusarinine-type siderophores ( 6 , 9 , 14 , 20 ), had higher TLs in conidia (0-h...”
- Nitrogen, Iron and Zinc Acquisition: Key Nutrients to Aspergillus fumigatus Virulence
Perez-Cuesta, Journal of fungi (Basel, Switzerland) 2021 - “...Decreased [ 59 ] Acetyltransferase Afu3g03650 sidG Triacylfusarinine non-productive Normal [ 59 ] Mevalonyl-CoA hydratase Afu3g03410 sidH Extracellular siderophores non-productive Decreased [ 60 ] Mevalonyl-CoA ligase Afu1g17190 sidI Extracellular siderophores non-productive Decreased [ 60 ] Fusarinine C esterase Afu3g03390 sidJ Viable Unknown [ 61 ] GNAT-type...”
- Aspergillus Fumigatus ZnfA, a Novel Zinc Finger Transcription Factor Involved in Calcium Metabolism and Caspofungin Tolerance
Valero, Frontiers in fungal biology 2021 - “...for virulence Iron homeostasis Afu7g06060 2.90909 sit1 Putative siderophore transporter; SrbA-regulated during hypoxia Iron homeostasis Afu3g03410 2.73843 sidH Enoyl-CoA hydratase/isomerase family protein; mevalonyl-CoA hydratase; PTS1 receptor-mediated peroxisomal localization is essential for triacetylfusarinine C (TAFC) biosynthesis Iron homeostasis Afu6g12870 3.04948 atm1 ABC iron exporter; transcript up-regulated in...”
- Genomic Organization and Expression of Iron Metabolism Genes in the Emerging Pathogenic Mold Scedosporium apiospermum
Le, Frontiers in microbiology 2018 - “...SidF Hydroxyornithine transacylase AFUA_3G03400 SAPIO_CDS2803 (6e-125/47%) 88 NW_015971788.1 * SidG Transacetylase AFUA_3G03650 SidH Mevalonyl-CoA hydratase AFUA_3G03410 SAPIO_CDS2272 (4e-80/48%) 97 NW_015971787.1 * SidI Mevalonyl-CoA ligase AFUA_1G17190 SAPIO_CDS2805 (2e-143/64%) 96 KEZ44717.1 SidL Transacetylase AFUA_1G04450 SAPIO_CDS2796 (7e-120/42%) 100 KEZ44711.1 EstB Triacetylfusarinine C esterase AFUA_3G03660 SidJ Lipase/Esterase AFUA_3G03390 PptA Phosphopantetheinyl...”
- Functional Investigation of Iron-Responsive Microsomal Proteins, including MirC, in Aspergillus fumigatus
Mulvihill, Frontiers in microbiology 2017 - “...biosynthesis were also shared; mevalonyl-CoA ligase SidI (AFUA_1G17190), transacylase SidF (AFUA_3G03400), and mevalonyl-CoA hydratase SidH (AFUA_3G03410). Strikingly, 27 proteins associated with the ribosome showed differential abundance in mirC compared with WT during iron-replete growth (Tables S8 , S9 ). Of the 19 ribosome-associated proteins with increased...”
- Regulation of Secondary Metabolism by the Velvet Complex Is Temperature-Responsive in Aspergillus
Lind, G3 (Bethesda, Md.) 2016 - “..., Afu3g03330 , Afu3g03340 , Afu3g03350 , Afu3g03370 , Afu3g03380 , Afu3g03390 , Afu3g03400 , Afu3g03410 , Afu3g03420 , Afu3g03430 , Afu3g03440 , Afu3g03445 , Afu3g03450 , Afu3g03460 Inglis et al. (2013) Cluster 13 Hexadehydroastechrome (HAS) cluster Afu3g12890 , Afu3g12900 , Afu3g12910 , Afu3g12920 , Afu3g12930...”
- RNAseq analysis of Aspergillus fumigatus in blood reveals a just wait and see resting stage behavior
Irmer, BMC genomics 2015 - “...FRE family ferric-chelate reductase, metalloreductase involved in response to iron starvation; reductive iron assimilation, freB AFUA_3G03410 sidH enoyl-CoA hydratase/isomerase family protein, mevalonyl-CoA hydratase; is essential for (TAFC) biosynthesis AFUA_3G03400 sidF siderophore biosynthesis acetylase AceI, hydroxyornithine transacylase; involved in extracellular siderophore biosynthesis; essential for TAFC biosynthesis AFUA_3G03420...”
- “...MirB), AFUA_3G03440 (MFS siderophore iron transporter), AFUA_7G06060 (siderochrome-iron transporter Sit1), AFUA_1G17270 (FRE family ferric-chelate reductase), AFUA_3G03410 (enoyl-CoA hydratase/isomerase family protein SidH), AFUA_3G03400 (siderophore biosynthesis acetylase AceI), AFUA_3G03420 (nonribosomal peptide synthase SidD), AFUA_3G03430 (ABC multidrug transporter SitT), AFUA_3G03440 (MFS siderophore iron transporter), and AFUA_2G07680 (L-ornithine N5-oxygenase SidA)...”
- Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals
Gressler, eLife 2015 - “...43%/59% SidF Transacylase AFUA_3G03400 Fe ATEG_05075 52%/67% SidG Transacetylase AFUA_3G03650 Fe none SidH Mevalonyl hydratase AFUA_3G03410 Fe ATEG_01509 53%/67% SidI Mevalonyl ligase AFUA_1G17190 Fe ATEG_05074 86%/91% SidL Transacetylase AFUA_1G04450 ATEG_03770 64%/76% Siderophore transporter (SIT) MirA Enterobactin transporter AN7800; - Fe ATEG_04071 68%/77% MirB TAFC transporter AN8540;...”
- More
crt1 / A5N5C7 crotonase (EC 4.2.1.150) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680) (see paper)
31% identity, 97% coverage
A4U99_15055 enoyl-CoA hydratase-related protein from Flavonifractor plautii
34% identity, 95% coverage
Clocel_2976 short-chain-enoyl-CoA hydratase from Clostridium cellulovorans 743B
32% identity, 97% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...to butyrate were down-regulated ( Figure 8 ). In addition, slight down-regulation of enoyl-CoA hydratase/isomerase (Clocel_2976) and butyrate kinase (Clocel_3674), although not statistically significant, provided a further evidence that the butyrate production pathway is repressed by butanol supplementation in C. cellulovorans . These data are inconsistent...”
- “...Clostridium Rex DNA binding element (TTGTTAANNNNTTAACAA) identified two motif instances upstream (23 and 88 from Clocel_2976 transcription start site) of the Clocel_2972-2976 gene cluster further supporting the hypothesis that Rex regulates its expression. Additional putative Rex binding motif instances were found upstream of acetyl-CoA acetyltransferase encoding...”
TTE0544 Enoyl-CoA hydratase/carnithine racemase from Thermoanaerobacter tengcongensis MB4
31% identity, 92% coverage
- Alcohol Selectivity in a Synthetic Thermophilic n-Butanol Pathway Is Driven by Biocatalytic and Thermostability Characteristics of Constituent Enzymes
Loder, Applied and environmental microbiology 2015 - “...Torrance, CA). Recombinant production of pathway enzymes. TTE0544 (crt), TTE0548 (hbd), TTE0549 (thl), Teth514_1935 (X514-bdh), and Teth514_1942 (X514-bad) were...”
- “...CA_P0035 CA_P0035 Cbei_3832 CA_C3298 TTE0549 TTE0548 TTE0544 STHERM_c16300 Cthe_0423 Teth514_0627 Teth514_1942 Teth514_1935 Homology to Enzyme query Sequence-...”
- Quantitative proteomics reveals the temperature-dependent proteins encoded by a series of cluster genes in thermoanaerobacter tengcongensis
Chen, Molecular & cellular proteomics : MCP 2013 - “...instance, the genes in the consecutively down-regulated mode, TTE0544, TTE0545, TTE0546, TTE0547, and TTE0549 were located within a 6 kilobase pairs region. To...”
Ccar_RS01400 short-chain-enoyl-CoA hydratase from Clostridium carboxidivorans P7
28% identity, 98% coverage
PA3591 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa PAO1
Q9HY35 Probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
34% identity, 98% coverage
- Static Growth Promotes PrrF and 2-Alkyl-4(1H)-Quinolone Regulation of Type VI Secretion Protein Expression in Pseudomonas aeruginosa
Brewer, Journal of bacteriology 2020 (secret) - Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...PA1535, PA1576, PA1628-PA1629, PA1631, PA1827, PA1869, PA2550, PA2552, PA2815, PA2841, PA2887-PA2891, PA2893, PA3286, PA3426, PA3589, PA3591, PA3593, PA3924,PA4089, PA4330, PA4912, PA4979-PA4980, PA4995, PA5020, PA5524 Protein and Amino acid metabolism thrS, folC, glnA, gmk, tgt, dadA, pauA3A5, gltX, gcvT1T2, glyQ, gdhA, valS, purD, trmD, speA, metGK, hutH,...”
- “...(Figure 5A ). While, microarray analysis showed that 8 genes encoding putative enoyl-CoA hydratase/isomerase ( PA3591, PA1021, PA1629, PA2890, PA3426, PA4330, PA1240 , and PA2841 ) were highly up-regulated in our study, known PPI data showed that only PA4980 directly interacts with a sensor kinase and...”
- Cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in Acinetobacter sp. strain ADP1
Parke, Applied and environmental microbiology 2001 - “...indicated parenthetically: dcaA homolog (PA3593), dcaE homolog (PA3591), dcaH homolog (PA3590), dcaF homolog (PA3589), and the possible porin gene (PA3588). In...”
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at least 83 amino...”
Ccar_22780, Ccar_RS22775 short-chain-enoyl-CoA hydratase from Clostridium carboxidivorans P7
28% identity, 96% coverage
- Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO2 and H2
Lauer, Microbial cell factories 2022 - “...butyryl-CoA/hexanoyl-CoA biosynthesis. Two homologous sets of these genes are present in the C. carboxidivorans genome: Ccar_RS22775 Ccar_RS22800 (Ccar1) and Ccar_RS01400 Ccar_RS01430 (Ccar2). We therefore amplified them from genomic DNA and placed them under the control of the C. acetobutylicum constitutive thlA promoter or the C. ljungdahlii...”
- Combination of Trace Metal to Improve Solventogenesis of Clostridium carboxidivorans P7 in Syngas Fermentation
Han, Frontiers in microbiology 2020 - “...fdhIV Formate dehydrogenase Ccar_16050 fdhV Formate dehydrogenase Ccar_22795 bcd Butyryl-CoA dehydrogenase Butyral-CoA synthesis from acetyl-CoA Ccar_22780 crt Crotonase Ccar_22785 hbd 3-Hydroxybutyryl-CoA dehydrogenase Ccar_22790 thl Acetyl-CoA acetyltransferase Ccar_22800 etfB Electron transfer flavoprotein Ccar_22805 etfA Electron transfer flavoprotein subunit alpha Ccar_00690 pta Phosphate acetyltransferase Acetate synthesis from acetyl-CoA...”
- “...etfA , and etfB . These six genes were clustered together on the chromosome from Ccar_22780 to Ccar_22805 and were all dramatically up-regulated under the Mo (0x) condition compared to levels observed in the Mo (1x) condition ( Figure 5B ). Two adjacent CDSs (Ccar_19515 and...”
dcaE / AAL09093.1 DcaE from Acinetobacter baylyi (see 11 papers)
28% identity, 94% coverage
Q9I393 Probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA1629 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa PAO1
34% identity, 92% coverage
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at least 83...”
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...). While, microarray analysis showed that 8 genes encoding putative enoyl-CoA hydratase/isomerase ( PA3591, PA1021, PA1629, PA2890, PA3426, PA4330, PA1240 , and PA2841 ) were highly up-regulated in our study, known PPI data showed that only PA4980 directly interacts with a sensor kinase and its response...”
- Characterization of molecular mechanisms controlling fabAB transcription in Pseudomonas aeruginosa
Schweizer, PloS one 2012 - “...encoding the 54 factor rpoN ; PA0286 ( desA ), encoding aerobic fatty acid desaturase; PA1629 , encoding a probable enoyl-CoA hydratase/isomerase; PA4760 , encoding a putative heat shock protein; PA4233 , encoding a probable major facilitator superfamily (MFS) transporter; and several other genes e.g. PA0358...”
- “...M 54 transcription factor # 16 PA4760 ::Tn M Heat shock protein DnaJ # 25 PA1629 ::Tn M Probable enoyl-CoA hydratase/isomerase # 30 PA0358 ::Tn M Hypothetical protein # 39 PA3649 ::Tn M Hypothetical protein (63% similar to E. coli yaeL ) # 44 PA4476 ::Tn...”
- In vivo evidence of Pseudomonas aeruginosa nutrient acquisition and pathogenesis in the lungs of cystic fibrosis patients
Son, Infection and immunity 2007 - “...PC PC PC Fatty acid degradation PA0507 PA1284 PA1628 PA1629 PA2889 PA2893 PA3013 PA3014 PA3300 PA3454 PA4199 PA4785 PA4814 PA4994 PA4995 fadE fadE fadB fadB...”
- Cloning and genetic characterization of dca genes required for beta-oxidation of straight-chain dicarboxylic acids in Acinetobacter sp. strain ADP1
Parke, Applied and environmental microbiology 2001 - “...for the PAO1 genes are indicated in parentheses: dcaE (PA1629), dcaH (PA1628), dcaR (PA1630), and dcaA (PA1631). In Acinetobacter, dcaR is linked to dcaF and...”
Q39VG6 (E)-2-benzylidenesuccinyl-CoA hydratase from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
34% identity, 94% coverage
Q70IM9 Putative enoyl-CoA hydratase I from Pseudomonas sp. Y2
34% identity, 92% coverage
bbsH / Q9KJE7 (2S)-2-[(R)-hydroxybenzyl]succinyl-CoA monomer (EC 4.2.1.180) from Thauera aromatica (see 2 papers)
BBSH_THAAR / Q9KJE7 (E)-benzylidenesuccinyl-CoA hydratase; EC 4.2.1.180 from Thauera aromatica (see 2 papers)
33% identity, 96% coverage
- function: Involved in an anaerobic toluene degradation pathway (PubMed:34601806). Catalyzes the hydration of (E)-2- benzylidenesuccinyl-CoA to the corresponding alcohol intermediate, 2- (alpha-hydroxybenzyl)succinyl-CoA (PubMed:34601806). Also accepts the N-acetylcysteamine (NAC) thioester of (E)-benzylidenesuccinate (PubMed:34601806).
catalytic activity: (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA = (E)-2- benzylidenesuccinyl-CoA + H2O (RHEA:70227)
subunit: Homotrimer.
H16_A1889 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
34% identity, 95% coverage
D2.1 / CAJ14152.1 putative dodecenoylCoA deltaisomerase, partial from Anopheles gambiae (see paper)
35% identity, 68% coverage
Msed_2001 / A4YI89 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) (see 2 papers)
HPCD_METS5 / A4YI89 3-hydroxypropionyl-coenzyme A dehydratase; 3-hydroxypropionyl-CoA dehydratase; EC 4.2.1.116 from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) (see paper)
A4YI89 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) from Metallosphaera sedula (see 3 papers)
5zaiC / A4YI89 Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
WP_012021928 3-hydroxypropionyl-CoA dehydratase from Metallosphaera prunae
Msed_2001 Enoyl-CoA hydratase/isomerase from Metallosphaera sedula DSM 5348
28% identity, 96% coverage
- function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl-CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crotonyl- CoA. Inactive towards (R)-3-hydroxybutyryl-CoA.
catalytic activity: 3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O (RHEA:26518)
subunit: Monomer. - Ligand: coenzyme a (5zaiC)
- Structural Insight into Substrate Specificity of 3-Hydroxypropionyl-Coenzyme A Dehydratase from Metallosphaera sedula.
Lee, Scientific reports 2018 - GeneRIF: Study reported Metallosphaera sedula 3-hydroxypropionyl-CoA dehydratase (Ms3HPCD) crystal structure in complex with Coenzyme A (CoA). Compared with the other enoyl-CoA hydratase family enzymes Ms3HPCD has a tightly formed alpha3 helix near the active site, and bulky aromatic residues are located at the enoyl-group binding site.
- A Novel Gene Cluster Is Involved in the Degradation of Lignin-Derived Monoaromatics in Thermus oshimai JL-2
Chakraborty, Applied and environmental microbiology 2021 (secret) - Genome analysis of the thermoacidophilic archaeon Acidianus copahuensis focusing on the metabolisms associated to biomining activities
Urbieta, BMC genomics 2017 - “...3-hydroxybutyryl-CoA dehydrogenase hpcD-hbd A4YDS4 651 EZQ11226 661 0 49 + (91) Bifunctional crotonyl-CoA hydratase/3-hydroxybutyryl-CoA dehydrogenase A4YI89 259 EZQ04864 257 2.00E -133 71 + (15) 3-hydroxypropionyl-coenzyme A dehydratase The proteins are identified by their NCBI accession number ( A. copahuensis ) or Uniprot identification number ( M....”
- Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways
Santos, Journal of advanced research 2023 - “...thus essential for the functioning of the 3-hydroxypropionate/4-hydroxybutyrate cycle [84] . In Metallosphaera sedula , Msed_2001 catalyzes the same reaction as Nmar_1028, and these two enzymes are homologous and have evolved independently from their respective bacterial homologs [15] . The existence of the HP/HB cycle in...”
- Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Liu, mSphere 2021 - “...both reactions are catalyzed in the aforementioned archaeal groups by a promiscuous 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase (Msed_2001 in crenarchaeon Metallosphaera sedula and Nmar_1308 in thaumarchaeon Nitrosopumilus maritimus ). Although these two enzymes are homologous, they are closely related to bacterial enoyl-CoA hydratases and were retrieved independently from...”
- “...Fig.1 ). In M. sedula , two dehydratases/hydratases have been characterized, (i) 3-hydroxypropionyl-CoA dehydratase (3HPD) Msed_2001 that acts equally well as crotonyl-CoA hydratase, and (ii) a bifunctional fusion protein crotonyl-CoA hydratase/( S )-3-hydroxybutyryl-CoA dehydrogenase Msed_0399; they are both present in, and were purified from, autotrophically grown...”
- Structural insights into bifunctional thaumarchaeal crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase from Nitrosopumilus maritimus
Destan, Scientific reports 2021 - “...addition, it was recently discovered that the Nmar_1308 homolog in the crenarchaeon Metallosphaera sedula (5ZA1; Msed_2001), previously characterized as 3HPD 23 , can also function as a CCAH 22 , 23 . Despite the critical role of Nmar_1308 protein in this aerobic archaeal CO 2 fixation...”
- “...5JBX) superposed with the Nmar_1308 structure with the indicated root mean squared deviation (RMSD) values. Msed_2001 (PDB IDs: 5ZAI) is shown as bifunctional 22 , 26 . It is notable that the RMSD value between Msed_2001 and Nmar_1308 is quite small, 0.91, the lowest among the...”
- Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula
Liu, Frontiers in microbiology 2020 - “...Msed_1456 Msed_1456 0.24 18 g / Up 0.7 Alber et al., 2008 3-Hydroxypropionyl-CoA dehydratase (5) Msed_2001 Msed_2001 0.24 /151 f,g /272 f,g No 0.04 Teufel et al., 2009 ; Loder et al., 2016 Acryloyl-CoA reductase (6) Msed_1426 Msed_1426 0.24 /7.6 f,g /18.7 f,g,h Up 0.9 Teufel...”
- “...2013a ; Ai et al., 2019 ). The studied crotonases are homologous to 3-hydroxypropionyl-CoA dehydratase Msed_2001 and possess low 3-hydroxypropionyl-CoA dehydratase activity ( Table 2 ), thus probably also contributing to 3-hydroxypropionyl-CoA dehydratase activity in the cells. 3-Hydroxypropionyl-CoA dehydratase, in turn, has crotonase activity ( Teufel...”
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea
Loder, Metabolic engineering 2016 - “...expression, and purification of HPCS, HPCD, ACR, MCR, and SSR The genes hpcs (Msed_1456), hpcd (Msed_2001), acr (Msed_1426), mcr (Msed_0709), and ssr (Msed_1424) were amplified from genomic DNA using the primers listed in Supplementary Table S1 . The genes hpcs, acr and ssr were ligated into...”
- “...ACR and SSR by Yeast Two Hybrid (A) Genomic context of HPCS (Msed_1456) and SSR (Msed_2001); ACR (Msed_1426) and SSR (Msed_1424). (B) Yeast two hybrid analyses of HPCS and HPCD, ACR and SSR. For HPCS and HPCD assay, Vector AD and BK, AD and BK-Msed_2001, AD-Msed_1456...”
- Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Hawkins, Applied and environmental microbiology 2014 - “...Msed_0148, Msed_1375 Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639 Msed_0638, Msed_2055 Msed_1424 Msed_0406 Msed_1321 Msed_0399 Msed_0656 NCE...”
- Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea
Hawkins, The Journal of biological chemistry 2013 - “...Msed_0148 Msed_1375 Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639 Msed_0638 Msed_2055 Msed_1424 Msed_0394 Msed_0406 Msed_1321 Msed_0399 Msed_0656...”
- Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle
Han, Applied and environmental microbiology 2012 - “...Msed_0148() Msed_1375() Msed_0709 Msed_1993 Msed_1456 Msed_2001 Msed_1426 Msed_0639 Msed_0638 Msed_2055 Acetyl-CoA/propionyl-CoA carboxylase Native (28, 44)...”
- More
CBO3202 3-hydroxybutyryl-CoA dehydratase from Clostridium botulinum A str. ATCC 3502
27% identity, 95% coverage
- Heat shock and prolonged heat stress attenuate neurotoxin and sporulation gene expression in group I Clostridium botulinum strain ATCC 3502
Selby, PloS one 2017 - “...motility ( sigD , cheA , and flgE ), and carbon metabolism ( cbo3199 , cbo3202 ), and included genes showing significantly activated or suppressed, or unaffected transcription levels in the microarray analysis. A linear regression analysis revealed a strong correlation between the log 2 fold-changes...”
- “...of these compounds in heat stressed C . botulinum culture. Two of these genes ( cbo3202 and cbo3199 ) were linked also to low-temperature stress response of ATCC 3502 at 17C [ 65 ]. Interestingly, butyrate in the growth medium has been shown to induce toxin...”
- The cold-induced two-component system CBO0366/CBO0365 regulates metabolic pathways with novel roles in group I Clostridium botulinum ATCC 3502 cold tolerance
Dahlsten, Applied and environmental microbiology 2014 - “...base 467 in antisense orientation, erm Insertional disruption of cbo3202 at base 167 in antisense orientation, erm ATCCa 15 This study This study This study...”
- “...with L1.LtrB retargeted to base 167 of cbo3202 in antisense orientation Recombinant protein expression vector, kanR pET-28b() harboring cbo0365 with N-terminal...”
- Transcriptomic analysis of (group I) Clostridium botulinum ATCC 3502 cold shock response
Dahlsten, PloS one 2014 - “..., cbo1407 , cbo2226 , cbo2227 , cbo2525 , cbo2847 , cbo2961 , cbo3199 and cbo3202 one hour after the cold shock, normalized to 16S rrn transcript levels and calibrated to pre-cold-shock transcript levels, were calculated using the Cq values obtained from qPCR runs. The Cq...”
- “...previous study [13] for genes cbo0751, cbo0753, cbo1407, cbo2226, cbo2227, cbo2525, cbo2847, cbo3199 , and cbo3202 ; all other data were produced in the current study. In a linear regression analysis between the microarray and RT-qPCR log 2 fold changes ( Fig. 2 ), a R...”
Q6CF43 YALI0B10406p from Yarrowia lipolytica (strain CLIB 122 / E 150)
32% identity, 71% coverage
VPARA_05530 enoyl-CoA hydratase/isomerase family protein from Variovorax paradoxus
31% identity, 93% coverage
- The catabolism of 3,3'-thiodipropionic acid in Variovorax paradoxus strain TBEA6: A proteomic analysis
Heine, PloS one 2019 - “...E . coli expression vector (C-terminal His-tag, Amp r , T7 promoter) expressing ech -30 (VPARA_05530) This study pET32a(+)::e ch-20 E . coli expression vector (C-terminal His-tag, Amp r , T7 promoter) expressing ech -20 (VPARA_05520) This study To determine the growth phase of liquid cell...”
- “...genes (VPARA_21730, VPARA_27740, VPARA_27760), no connections to the TDP catabolism were identified. Enoyl-CoA hydratases (VPARA_05520, VPARA_05530) and a crotonase family protein (VPARA_05510) Ech-20 and Ech-30 were of special interest, as they seemed to be involved in TDP metabolism. Deletion mutants lacking one or both ech genes...”
DMB42_RS42710 enoyl-CoA-hydratase DpgD from Nonomuraea sp. WAC 01424
35% identity, 97% coverage
D3RXI4 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); 3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) from Ferroglobus placidus (see paper)
30% identity, 37% coverage
AWY96_RS24510 2,3-dehydroadipyl-CoA hydratase PaaF from Serratia plymuthica
32% identity, 96% coverage
BAB1_2185 Enoyl-CoA hydratase/isomerase from Brucella melitensis biovar Abortus 2308
31% identity, 96% coverage
- Intracellular adaptation of Brucella abortus
Lamontagne, Journal of proteome research 2009 - “...synthesis BA14 lectin like 73 BAB1_0483 Lipid metabolism FabG 74 BAB1_0486 Lipid metabolism FabF 75 BAB1_2185 Lipid metabolism Ech 76 BAB1_2174 Lipid metabolism FabA 77 BAB2_0975 Lipid metabolism FabC 78 BAB2_1008 Cell division MepA 79 BAB1_1530 Cell division UvrB 80 BAB2_0475 Cell division XseA 81 BAB1_0640...”
BMEI1945 enoyl-CoA hydratase from Brucella melitensis 16M
33% identity, 96% coverage
echA mitochondrial enoyl-CoA hydratase from Emericella nidulans (see paper)
30% identity, 83% coverage
- CharProtDB Description: Mitochondrial enoyl-CoA hydratase, involved in fatty acid beta-oxidation; required for growth on short-chain fatty acids and for catabolism of isoleucine and valine; transcription is induced by fatty acids; Source:AspGD
I6J59_05415 short-chain-enoyl-CoA hydratase from Butyricimonas virosa
28% identity, 97% coverage
Afu2g10920 enoyl-CoA hydratase/isomerase family protein from Aspergillus fumigatus Af293
29% identity, 85% coverage
- Evolutionary Analysis of Sequence Divergence and Diversity of Duplicate Genes in Aspergillus fumigatus
Yang, Evolutionary bioinformatics online 2012 - “...1.49 [1.57, 0.69] Afu5g12770 2.20 [2.17, 1.20] Afu1g00540 1.54 [1.78, 1.00] Afu8g04060 1.74 [1.85, 0.86] Afu2g10920 1.57 [2.32, 2.09] Afu3g03410 1.95 [2.24, 1.52] Afu8g04070 1.66 [1.85, 0.92] Afu1g00480 2.98 [2.01, 0.19] Afu3g07830 1.67 [2.27, 1.88] Afu1g06710 2.55 [2.08, 0.69] Afu2g10840 1.81 [1.29, 0.01] Afu6g11810 2.47 [1.61,...”
- Genes differentially expressed in conidia and hyphae of Aspergillus fumigatus upon exposure to human neutrophils
Sugui, PloS one 2008 - “...family AN6752.2 Afu7g06090 fatty-acyl coA oxidase (Pox1) AN6752.2 Afu4g10950 3-ketoacyl-coA thiolase peroxisomal A precursor AN5646.2 Afu2g10920 enoyl-CoA hydratase/isomerase family protein AN5916.2/ echA Acetate metabolism: Afu1g13510 C6 transcription factor (FacB/Cat8) AN0689.2/ facB Afu4g11080 acetyl-coenzyme A synthetase FacA AN5626.2/ facA Afu6g14100 mitochondrial carnitine:acyl carnitine carrier AN5356.2/ acuH Afu1g12340...”
CBY_3041 3-hydroxybutyryl-CoA dehydratase from Clostridium butyricum 5521
30% identity, 94% coverage
- Reduced catabolic protein expression in Clostridium butyricum DSM 10702 correlate with reduced 1,3-propanediol synthesis at high glycerol loading
Gungormusler-Yilmaz, AMB Express 2014 - “...0.36 0.59 0.00 0.22 CBY_2920 butyrate kinase 0.62 0.54 0.89 0.91 0.26 0.32 0.38 0.33 CBY_3041 3-hydroxybutyryl-CoA dehydratase 0.50 0.29 1.17 1.11 NQ NQ NQ NQ CBY_3042 acyl-coa dehydrogenase (short-chain specific) 0.20 0.25 0.90 0.60 0.06 0.06 0.96 0.94 CBY_3045 3-hydroxybutyryl-coa dehydrogenase 0.24 0.20 1.16 1.28...”
- “...CBY_1889 and 3642), FeFe hydrogenase (CBY_2300), acetyl CoA acetyltransferase (CBY_1290), 3-hydroxybutyryl-CoA dehydrogenase (CBY_3045), 3-hydroxybutyryl-coa dehydratase (CBY_3041), acyl-CoA dehydrogenase (CBY_3258 and 3042), phosphate butyryltransferase (CBY_2919), butyrate kinase (CBY_2920), butanol dehydrogenase (CBY_3747 and 3751), phosphate acetyltransferase (CBY_0205), acetate kinase (CBY_0206), lactate dehydrogenase (CBY_0742, 2341 and 2757), and ethanol...”
GAH_01602 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein from Geoglobus ahangari
32% identity, 40% coverage
- The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T)
Manzella, Standards in genomic sciences 2015 - “...hydratase, which in G. ahangari could be encoded by 4 genes (GAH_00487, GAH_00802, GAH_01332, and GAH_01602). Two of these genes (GAH_00487 and GAH_01602) are in fact hybrid proteins containing an enoyl-CoA hydratase domain fused to a 3-hydroxyacyl-CoA dehydrogenase domain. Hybrid enoyl-CoA hydratase/dehydrogenase proteins such as these...”
- “...dehydrogenase protein, which in G. ahangari is likely encoded by several genes (GAH_00328, GAH_00487, GAH_01600, GAH_01602 again noting the hybrid nature of GAH_00487 and GAH_01602). Finally, acetyl-CoA is removed from the 3-oxo-acyl-CoA molecule by an acetyl-CoA acetyltransferase and is free to enter the TCA cycle. There...”
DPGD_AMYOR / G4V4T7 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis) (see paper)
35% identity, 97% coverage
- function: Involved in the biosynthesis of the nonproteinogenic amino acid monomer (S)-3,5-dihydroxyphenylglycine (Dpg) responsible of the production of vancomycin and teicoplanin antibiotics. Catalyzes the syn-addition of a water molecule across the double bond of a trans-2- enoyl-CoA thioester, resulting in the formation of a beta-hydroxyacyl- CoA thioester. Physiologically, DpgD could act as a dehydratase, facilitating the aromatization of the DPA-S-DgpA or DPA-S-CoA intermediate. It can use the non physiological substrates crotonyl-CoA and beta-methylcrotonyl-CoA.
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (RHEA:20724) - Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil.
Margesin, Applied microbiology and biotechnology 2021 - “...WP_028194277.1, WP_069266866.1, WP_064271658.1, WP_012431551.1, WP_012431843.1, WP_035551717.1, WP_012432712.1, WP_035997457.1, WP_011489469.1, WP_011489931.1, WP_012434439.1, SDH36466.1, SDB90530.1, SAL69706.1, SAL32406.1, G4V4T7, O34893 Chromosome 1, 2, and 3 Feruloyl-SCoA to vanillin and acetyl-SCoA (Gasson et al. 1998 ) 5.0e-66; 1.0e-130; 1.6e-201; 1.7e-163; 1.1e-58; 3.5e-123; 2.6e-135; 7.1e-52; 0; 2.4e-130; 1.5e-132; 4.0e-138; 4.2e-157; 1.6e-139;...”
XP_002791730 carnitinyl-CoA dehydratase from Paracoccidioides lutzii Pb01
33% identity, 85% coverage
FGSG_11295 hypothetical protein from Fusarium graminearum PH-1
31% identity, 91% coverage
F502_06297 short-chain-enoyl-CoA hydratase from Clostridium pasteurianum DSM 525 = ATCC 6013
28% identity, 94% coverage
Swol_0790 Enoyl-CoA hydratase/carnithine racemase from Syntrophomonas wolfei subsp. wolfei str. Goettingen
27% identity, 97% coverage
- Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei
Fu, Frontiers in microbiology 2022 - “...as follows: acyl-CoA transferase (Swol_0309, 1014, 1147, 2128), acyl-CoA dehydrogenase (Swol_0488, 0788, 1841), enoyl-CoA hydratase (Swol_0790, 2031, 2129), 3- hydroxybutyryl-CoA dehydrogenase (Swol_0307, 0791, 1171), and acetyl-CoA acetyltransferase (Swol_0308, 0789). Our improved proteomic depth revealed additional -oxidation paralogs and led us to investigate the presence of protein...”
PP_3284 enoyl-CoA hydratase/isomerase from Pseudomonas putida KT2440
34% identity, 92% coverage
- β-oxidation-polyhydroxyalkanoates synthesis relationship in Pseudomonas putida KT2440 revisited
Liu, Applied microbiology and biotechnology 2023 - “...KT2440 PhaJ homologues for the search, other hydratases, such as PP_3726, PP_1845, PP_2217, PP_2136 and PP_3284, PP_3491, PP_3925 were identified. We have detected PP_1845, PP_2217, PP_2136, PP_3925 and PP_3491 in the proteome of both the WT and sextuple mutant, albeit without a statistically significant change in...”
AUO97_RS14200 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG from Acinetobacter baumannii
28% identity, 97% coverage
XP_001123353 probable enoyl-CoA hydratase, mitochondrial from Apis mellifera
26% identity, 85% coverage
DSY1709 hypothetical protein from Desulfitobacterium hafniense Y51
30% identity, 94% coverage
ACICU_01342 enoyl-CoA hydratase/carnithine racemase from Acinetobacter baumannii ACICU
28% identity, 97% coverage
- Colistin Resistant A. baumannii: Genomic and Transcriptomic Traits Acquired Under Colistin Therapy
Cafiso, Frontiers in microbiology 2018 - “...the over-expressed DEGs mapped on A. baumannii ACICU RefGen, i.e. Butanoate metabolism (4 genes: ACICU_01343, ACICU_01342, ACICU_01735, ACICU_01767); Glycolysis/Gluconeogenesis (3 genes: ACICU_02656, ACICU_01735, ACICU_01737); Benzoate degradation via CoA ligation (3genes: ACICU_01343, ACICU_01342, ACICU_01767); Pyruvate metabolism (3 genes: ACICU_01735, ACICU_01737, ACICU_01767); Valine, leucine and isoleucine degradation (3...”
PFLU3030 putative phenylacetic acid degradation enoyl-CoA hydratase from Pseudomonas fluorescens SBW25
32% identity, 94% coverage
HFX_2830 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein from Haloferax mediterranei ATCC 33500
30% identity, 39% coverage
- Enoyl-CoA hydratase mediates polyhydroxyalkanoate mobilization in Haloferax mediterranei
Liu, Scientific reports 2016 - “...of the -oxidation pathway in PHA mobilization. The expression of four genes ( HFX_1509 , HFX_2830 , HFX_4016 , and HFX_6355 , Supplementary Information Table S3 ) that encode the predicted key enzyme, 3-hydroxyacyl-CoA dehydrogenase, in -oxidation cycle were investigated when the PHA-accumulated cells were cultured...”
- “...PD medium (PAC medium without glucose, the condition for PHA mobilization), the expression of gene HFX_2830 , HFX_4016 , and HFX_6355 was up-regulated clearly and enhanced gradually within the observed 10hours, while the expression of gene HFX_1509 was up-regulated in the first hour but decreased later...”
- Multiple propionyl coenzyme A-supplying pathways for production of the bioplastic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Haloferax mediterranei
Han, Applied and environmental microbiology 2013 - “...The enzymes (HFX_0862, HFX_1490, HFX_2726, and HFX_2830) that catalyze the conversion of 2-oxobutyrate to propionyl-CoA in the citramalate/2-oxobutyrate...”
- “...Citramalate/2-oxobutyrate and aspartate/2-oxobutyrate pathways HFX_0862 HFX_1490 HFX_2726 HFX_2830 korA acd/caiA acd/caiA fadB -2.1 0.2 -5.5 0.0 -1.9 0.2 -2.9...”
A9762_23740 enoyl-CoA hydratase from Pandoraea sp. ISTKB
31% identity, 95% coverage
rrnAC1083 3-hydroxyacyl-CoA dehydrogenase from Haloarcula marismortui ATCC 43049
31% identity, 38% coverage
- Genome information management and integrated data analysis with HaloLex
Pfeiffer, Archives of microbiology 2008 - “...Shortened HQ1663A, OE1669F rrnAC0956 462 537 Shortened HQ1497A, OE2934R rrnAC1042 1,806 1,851 Shortened rrnAC1570, HQ3533A rrnAC1083 1,965 2,010 Shortened NP4322A, OE2871F rrnAC1106 519 420 Extended NP4198A, OE2985F, HQ2561A rrnAC1107 1,476 1,176 Extended NP4904A, HQ1686A rrnAC1115 270 324 Shortened NP4036A, OE2903R, HQ2458A rrnAC1138 849 507 Extended OE2020F,...”
XALc_1063 hypothetical enoyl-coa hydratase/isomerase; protein from Xanthomonas albilineans
34% identity, 96% coverage
- Genome mining reveals the genus Xanthomonas to be a promising reservoir for new bioactive non-ribosomally synthesized peptides
Royer, BMC genomics 2013 - “...that may generate 3,5-dihydroxyphenylacetyl-CoA from four molecules of malonyl-CoA, with the assistance of XALc_1060 and XALc_1063 (which encode proteins DpgB and DpgD, respectively, and which may exhibit dehydratase activity). XALc_1061 is predicted to encode dioxygenase DpgC, which may convert 3,5-dihydroxyphenylacetyl-CoA into 3,5-dihydroxyphenyl-glyoxylate, and subsequently generate Dpg...”
- “...HpgT (CAC48367) 43% / 58% HpgT (AAM80549) 42% / 58% Transaminase, generates 3,5-dihydroxyphenyl-glycine from 3,5-dihydroxyphenylglyoxylate XALc_1063 DpgD (CAC48381) 62% / 74% DpgD (AAM80545) 61% / 74% Belongs to crotonase/Enoyl-CoA hydratase superfamily, enhances DpgA activity * This function was characterized for biosynthesis of vancomycin [ 43 ]....”
An02g02820 uncharacterized protein from Aspergillus niger
31% identity, 82% coverage
- Metabolic pathway reconstruction of eugenol to vanillin bioconversion in Aspergillus niger
Srivastava, Bioinformation 2010 - “...An01g09260 (gi|145230075|ref|XP_001389346.1 predicted as CalB), hypothetical protein An14g05630 (gi|145249694|ref|XP_001401186.1 predicted as Fcs) and hypothetical protein An02g02820 (gi|145231906|ref|XP_001399422.1 predicted as Ech) predicted to be the pathways enzymes of target organism i.e., A. niger with conserved protein domains & motifs (Table 1 in supplementary material ). Sequence similarity...”
X276_16680 short-chain-enoyl-CoA hydratase from Clostridium beijerinckii NRRL B-598
30% identity, 94% coverage
DEFDS_1835 3-hydroxybutyryl-CoA dehydratase from Deferribacter desulfuricans SSM1
31% identity, 90% coverage
P96404 Probable enoyl-CoA hydratase EchA1 (Enoyl hydrase) (Unsaturated acyl-CoA hydratase) (Crotonase) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
L7N5S5 Enoyl-CoA hydratase/isomerase family protein from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
NP_214736 enoyl-CoA hydratase EchA1 from Mycobacterium tuberculosis H37Rv
Rv0222 enoyl-CoA hydratase from Mycobacterium tuberculosis H37Rv
33% identity, 98% coverage
- Mycobacterium tuberculosis peptides presented by HLA-E molecules are targets for human CD8 T-cells with cytotoxic as well as regulatory activity
Joosten, PLoS pathogens 2010 - “...29 LMHYRGELL Rv1965 O53966 201 20 30 NMMARGMDL Rv0221 P96403 201 >50 31 LPAERAHEL Rv0222 P96404 202 >50 32 VMMSEIAGL Rv1813c P64889 348 13 33 TMITFRLRL Rv1733c P71991 373 2,4 34 VMTTVLATL Rv1734c P71992 348 1,8 35 GMGMVGTAL Rv1737c P71995 348 >50 36 AMAGSIDLL Rv2006 Q10850...”
- Activity-Based Protein Profiling Reveals That Cephalosporins Selectively Active on Non-replicating Mycobacterium tuberculosis Bind Multiple Protein Families and Spare Peptidoglycan Transpeptidases
Lopez, Frontiers in microbiology 2020 - “...Q7D7S1 Rv1925 fadD31 No PP: acyl-CoA synthase Q50824 Rv0685 desA1 Yes PP: putative acyl-[acyl-carrier-protein] desaturase L7N5S5 Rv0222 echA1 No SF: probable enoyl-CoA hydratase; crotonase P0A4W6 Rv2244 AcpM Yes SF: meromycolate extension acyl carrier protein Q11198 Rv3389c htdY No SF: 3-hydroxy acyl thioester dehydratase Ribosomal/transcriptional function P66044...”
- Host-mediated ubiquitination of a mycobacterial protein suppresses immunity.
Wang, Nature 2020 (PubMed)- GeneRIF: host E3 ubiquitin ligase ANAPC2 interacts with the mycobacterial protein Rv0222 and promotes the attachment of lysine-11-linked ubiquitin chains to lysine 76 of Rv0222 in order to suppress the expression of proinflammatory cytokines; identification of mechanism that M. tuberculosis uses to suppress host immunity, and provide insights relevant to the development of effective immunomodulators that target M. tuberculosis
- Identification of new diagnostic biomarkers for Mycobacterium tuberculosis and the potential application in the serodiagnosis of human tuberculosis
Ren, Microbial biotechnology 2018 - “...2010b ). Rv0222, a 263amino acid protein, is possibly an enoylCoA hydratase (GenBank accession no: NP_214736 ), which is conserved in most mycobacterial species. Although Rv0222 was previously used to detect TB, with a sensitivity of 82% in 38TB cases, including 29 PTB and 9 EPTB...”
- Improving sensitivity for serodiagnosis of tuberculosis using TB16.3-echA1 fusion protein.
Khurshid, Tuberculosis (Edinburgh, Scotland) 2014 (PubMed)- GeneRIF: Novel fusion protein TB16.3-echA1 has a potential in serodiagnosis of tuberculosis with improved sensitivity and reliability.
- Development and evaluation of a triplex droplet digital PCR method for differentiation of M. tuberculosis, M. bovis and BCG
Qu, Frontiers in microbiology 2024 (PubMed)- “...(targeting CFP-10-ESAT-6 gene of RD1 and Rv0222 genes of RD4), <italic>M. bovis</italic> (targeting CFP-10-ESATs-6 gene of RD1), and...”
- “...<i>M. tuberculosis</i> (targeting CFP-10-ESAT-6 gene of RD1 and Rv0222 genes of RD4), <i>M. bovis</i> (targeting CFP-10-ESATs-6 gene of RD1), and BCG (targeting...”
- Mycobacterium tuberculosis protein Rv2652c enhances intracellular survival by inhibiting host immune responses
Li, Immunity, inflammation and disease 2024 - “...through the inhibition of p38 MAPK and NFB signaling cascades. 24 On the other hand, Rv0222 has been demonstrated to subvert innate immune responses by targeting the JNK, p38 MAPK, and NFB signaling pathways. 25 Furthermore, Mce3E effectively inhibits the ERK1/2 signaling cascade, consequently suppressing the...”
- In silico design of Mycobacterium tuberculosis multi-epitope adhesin protein vaccines
Pillay, Heliyon 2024 - “...interaction. The authors further showed that the fusion of the truncated PstS1 to the EchA1 (Rv0222) proteins displayed an improved serodiagnosis of TB [ 112 ]. 4.2 Two trifusion protein constructs with multi-epitope regions lacking a signal peptide with good population coverage Common B-cell epitope regions...”
- From pathogenesis to antigens: the key to shaping the future of TB vaccines
Yang, Frontiers in immunology 2024 - “...of TNF-, IL-6, and NO via interacting with macrophage ubiquitin ligase (E3) ( 44 ) Rv0222 Through interaction with the host E3 ubiquitin ligase ANAPC2, Rv0222 prevents ubiquitination and activation of TRAF6 by facilitating the binding of the protein tyrosine phosphatase SHP1 to the adaptor protein...”
- Mycobacterial Rv1804c binds to the PEST domain of IκBα and activates macrophage-mediated proinflammatory responses
Zheng, iScience 2024 - “...NF-B and AP-1 signaling pathways by interacting with makorin ring finger protein 1. 13 Mtb Rv0222 suppresses the expression of proinflammatory cytokines by interacting with anaphase-promoting complex subunit 2, an E3 ubiquitin ligase, in the host. 14 Mtb PPE36 inhibits host inflammatory responses and increases bacterial...”
- Research progress of single-cell sequencing in tuberculosis
Pan, Frontiers in immunology 2023 - “...residues on the bacterial cell surface ( 8 ), Mtb can secrete the virulence factor Rv0222 to escape the immune response after infecting the human body ( 9 ). However, after infection, a comprehensive panorama of the intricate host immune regulatory processes and dynamic evolution still...”
- The exploitation of host autophagy and ubiquitin machinery by Mycobacterium tuberculosis in shaping immune responses and host defense during infection
Shariq, Autophagy 2023 (secret) - Mycobacterium tuberculosis-macrophage interaction: Molecular updates
Bo, Frontiers in cellular and infection microbiology 2023 - “...etal., 2022 ). This inhibition of host immunity was also reported following lysine-11-linked ubiquitination of Rv0222 by the host E3 ubiquitin ligase ANAPC2 involving SHP1 and TRAF6, or deubiquitination of TRAF6 by HAUSP after PE_PGRS38 ectopic expression in M. smegmatis in murine bone marrow-derived macrophages (...”
- More
lpp0932 hypothetical protein from Legionella pneumophila str. Paris
30% identity, 93% coverage
Cbei_2034 3-hydroxybutyryl-CoA dehydratase from Clostridium beijerincki NCIMB 8052
30% identity, 94% coverage
Dred_1781 Enoyl-CoA hydratase/isomerase from Desulfotomaculum reducens MI-1
29% identity, 96% coverage
SPA0071 carnitine racemase from Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150
SC0064 carnitine racemase from Salmonella enterica subsp. enterica serovar Choleraesuis str. SC-B67
34% identity, 95% coverage
CIBE_0339, X276_25220 short-chain-enoyl-CoA hydratase from Clostridium beijerinckii
29% identity, 94% coverage
- Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level
Patakova, Scientific reports 2019 - “...crt )- butyryl-CoA dehydrogenase ( bcd ) etfB etfA - 3-hydroxybutyryl-CoA dehydrogenase ( hbd ), (X276_25220 - X276_25200). Surprisingly, Genome2D operon prediction suggested another operon organisation and placed the last gene hbd (X276_25200) into a separate operon (operon_0155). Simultaneously, expression profiles showed high correlation of the...”
- Genome and transcriptome of the natural isopropanol producer Clostridium beijerinckii DSM6423
Máté, BMC genomics 2018 - “...dedicated to the production of butyryl-CoA from acetoacetyl-CoA seems to be organized in an operon (CIBE_0339 to 0343, Fig. 4 ) and are not regulated in our fermentation conditions. More interestingly, the 3 genes (CIBE_5186, CIBE _2196 and CIBE _1684) apparently involved in the pyruvate decarboxylation...”
TTHA1434 3-hydroxybutyryl-CoA dehydratase from Thermus thermophilus HB8
35% identity, 90% coverage
STM0070 carnitine racemase from Salmonella typhimurium LT2
34% identity, 95% coverage
Swol_2031 3-hydroxybutyryl-CoA dehydratase from Syntrophomonas wolfei subsp. wolfei str. Goettingen
29% identity, 95% coverage
- Dynamic acylome reveals metabolite driven modifications in Syntrophomonas wolfei
Fu, Frontiers in microbiology 2022 - “...Swol_2126 117 K2(Acetyl) AKHIVSFAFTEPGTGSDPK 2 Swol_2126 214 K3(Acetyl) DVKVPADNLLGK 2 Swol_2126 273 K6(Acetyl) GQPIAKFQAIQLK 3 Swol_2031 197 K18(Acetyl) IGLVNHVYPADQLMDEAKK 3 Swol_2031 202 K4(Acetyl, 3- Hydroxybutyryl) IANKAPLAVGYAK 3 Swol_2,129 15 K3(Acetyl) LEKGILLVSLNRPEK 4 Swol_0435 52, 56 K1(Acetyl); K5(Butyryl) KAVEKGK 4 Swol_0435 172 K23(Acetyl) LVEVIPGAETSEAVSSAIVELCKK 4 Swol_0791 28...”
FNP_0790 enoyl-CoA hydratase-related protein from Fusobacterium polymorphum ATCC 10953
29% identity, 96% coverage
- Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium
Karpathy, PloS one 2007 - “...in S. flexneri [74] , [75] . The strain also carries genes for butyrate fermentation (FNP_0790, 0791, 0969, 0970, 0971, 1762 and either 1467 or 2146). The production of butyrate has been associated with mouth odor and gingival inflammation [76] . We also include FipA (described...”
- “...domain protein FNP_1921 2263322 2265424 vacB ribonuclease R Butyrate fermentation FNP_2146 62092 60956 butyryl-CoA dehydrogenase FNP_0790 1158356 1159132 3-hydroxybutyryl-CoA dehydratase FNP_0791 1159148 1159987 fadB 3-hydroxybutyryl-CoA dehydrogenase FNP_0969 1326816 1326151 atoA butyrate-acetoacetate CoA-transferase, beta subunit FNP_0970 1327487 1326834 atoD butyrateacetoacetate CoA-transferase, alpha subunit FNP_0971 1329020 1327641 atoE...”
Cbei_4544 enoyl-CoA hydratase/isomerase from Clostridium beijerincki NCIMB 8052
28% identity, 95% coverage
AORI_1505 enoyl-CoA-hydratase DpgD from Amycolatopsis keratiniphila
34% identity, 96% coverage
- Complete genome sequence and comparative genomic analyses of the vancomycin-producing Amycolatopsis orientalis
Xu, BMC genomics 2014 - “...exploration. The vcm cluster was annotated to encode a total of 35 enzymes (AORI_1471 to AORI_1505), including three vancomycin-resistance proteins (VanH, VanA, and VanX [ 7 , 8 ]), three large NRPSs, several post-assembly tailoring enzymes, and a series of biosynthetic proteins for the supply of...”
- “...dpgA Polyketide synthase lcl|AJ223998.1_cdsid_CAA11765.1 92.9 AORI_1503 dpgB Isomerase lcl|Y16952.3_cdsid_CAC48379.1 75 AORI_1504 dpgC Thioesterase lcl|Y16952.3_cdsid_CAC48380.1 83.25 AORI_1505 dpgD Dehydration protein lcl|Y16952.3_cdsid_CAC48381.1 86.49 Note: - represents no orthologous gene was found in bal (Y16952.3) or cep (AL078635.1, AJ223999.1 and AJ223998.1) clusters. Similar to the biosynthesis of balhimycin and...”
NP_650199 Spliceosome-ribosome linker protein from Drosophila melanogaster
30% identity, 53% coverage
TTX_1028 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein from Thermoproteus tenax Kra 1
31% identity, 37% coverage
GAH_00487 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein from Geoglobus ahangari
27% identity, 38% coverage
- The complete genome sequence and emendation of the hyperthermophilic, obligate iron-reducing archaeon "Geoglobus ahangari" strain 234(T)
Manzella, Standards in genomic sciences 2015 - “...catalyzed by an enoyl-CoA hydratase, which in G. ahangari could be encoded by 4 genes (GAH_00487, GAH_00802, GAH_01332, and GAH_01602). Two of these genes (GAH_00487 and GAH_01602) are in fact hybrid proteins containing an enoyl-CoA hydratase domain fused to a 3-hydroxyacyl-CoA dehydrogenase domain. Hybrid enoyl-CoA hydratase/dehydrogenase...”
- “...a 3-hydroxyl-CoA dehydrogenase protein, which in G. ahangari is likely encoded by several genes (GAH_00328, GAH_00487, GAH_01600, GAH_01602 again noting the hybrid nature of GAH_00487 and GAH_01602). Finally, acetyl-CoA is removed from the 3-oxo-acyl-CoA molecule by an acetyl-CoA acetyltransferase and is free to enter the TCA...”
ST0048 254aa long hypothetical enoyl-CoA hydratase from Sulfolobus tokodaii str. 7
29% identity, 96% coverage
- Macromolecular fingerprinting of sulfolobus species in biofilm: a transcriptomic and proteomic approach combined with spectroscopic analysis
Koerdt, Journal of proteome research 2011 - “...n.s. 0.69 0.075 Carbon monoxide dehydrogenase subunit G SSO2430 n.d. 1.19 0.029 Sulfurtransferase enoyl-CoA hydratase ST0048 2.01 n.s. Carbon monoxide dehydrogenase large chain Saci_2117 n.s. 0.51 0.002 SSO3009 n.d. 0.3 0.046 Oxidoreductase SSO2794 n.d. 0.32 0.021 Thiosulfate reductase electron transport protein (PhsB) ST1839 n.d. 0.67 0.022...”
- “...Anaerobic dimethylsulfoxide reductase ST1789 1.06 n.s. Putative thiosulfate sulfurtransferase ST2564 1.22 n.s. Sulfurtransferase enoyl-CoA hydratase ST0048 2.01 n.s. Carbohydrate transport and metabolism Sugar-related transporter Saci_1782 n.d. 0.59 0.381 SSO2057 n.d. 0.93 0.023 Sugar transporter Saci_2111 n.d. 0.71 0.006 SSO2716 n.d. 0.66 0.012 proline/betaine transporter SSO2938 n.d....”
SS1G_14001 enoyl-CoA hydratase/carnithine racemase from Sclerotinia sclerotiorum 1980 UF-70
29% identity, 89% coverage
BP1026B_I0261 enoyl-CoA hydratase from Burkholderia pseudomallei 1026b
32% identity, 95% coverage
- Transient In Vivo Resistance Mechanisms of Burkholderia pseudomallei to Ceftazidime and Molecular Markers for Monitoring Treatment Response
Cummings, PLoS neglected tropical diseases 2017 - “...are unknown hypotheticals (N = 14). Annotated genes of interest as potential biomarkers are paaF (BP1026B_I0261), tagD-4 (BP1026B_II0586), and filR (BP1026B_I0034). Together, the molecular markers of in vivo infection and the molecular markers discriminant of ceftazidime treatment provide the foundation for diagnostics about response to treatment....”
- “...markers ceftazidime treatment in vivo . Locus Tag Gene Name Product Name COG Mean FPKM BP1026B_I0261 paaF enoyl-CoA hydratase Lipid metabolism 7281 BP1026B_I1657 hypothetical protein Function unknown 3468 BP1026B_II0389 hypothetical protein Function unknown 2509 BP1026B_II2046 carboxylesterase family protein Lipid metabolism 1729 BP1026B_II1993 Function unknown 1715 BP1026B_II1217...”
ATEG_01509 uncharacterized protein from Aspergillus terreus NIH2624
31% identity, 90% coverage
- Phytotoxin production in Aspergillus terreus is regulated by independent environmental signals
Gressler, eLife 2015 - “...Transacylase AFUA_3G03400 Fe ATEG_05075 52%/67% SidG Transacetylase AFUA_3G03650 Fe none SidH Mevalonyl hydratase AFUA_3G03410 Fe ATEG_01509 53%/67% SidI Mevalonyl ligase AFUA_1G17190 Fe ATEG_05074 86%/91% SidL Transacetylase AFUA_1G04450 ATEG_03770 64%/76% Siderophore transporter (SIT) MirA Enterobactin transporter AN7800; - Fe ATEG_04071 68%/77% MirB TAFC transporter AN8540; -AFUA_3G03640 Fe...”
YaaL / b0036 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
caiD / P31551 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli (strain K12) (see 2 papers)
CAID_ECOLI / P31551 Carnitinyl-CoA dehydratase; Crotonobetainyl-CoA hydratase; EC 4.2.1.149 from Escherichia coli (strain K12) (see paper)
P31551 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Escherichia coli (see 2 papers)
caiD carnitinyl-CoA dehydratase; EC 4.2.1.- from Escherichia coli K12 (see 5 papers)
b0036 crotonobetainyl CoA hydratase from Escherichia coli str. K-12 substr. MG1655
32% identity, 95% coverage
- function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA (PubMed:11551212). Can also hydrate crotonyl-CoA to hydroxybutyryl-CoA (PubMed:11551212).
catalytic activity: (R)-carnitinyl-CoA = crotonobetainyl-CoA + H2O (RHEA:28338)
catalytic activity: 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:45584)
subunit: Homotrimer. - Genome annotation errors in pathway databases due to semantic ambiguity in partial EC numbers
Green, Nucleic acids research 2005 - “...EC 4.2.1.- appears in 10 KEGG pathway maps. In eight of these maps, two genes b0036 [Swiss-Prot entry P31551, Carnitinyl-CoA dehydratase (EC 4.2.1.-)] (example URL: ) and b1517 [Swiss-Prot entry P76143, Putative aldolase yneB (EC 4.2.1.-); hypothetical 31.9 kDa protein in hipBuxaB intergenic region] are both...”
- “...a set of 16 distinct reactions as shown in the Supplementary Materials. According to EcoCyc, b0036 and b1517 encode a carnitine racemase (EC 5.-.-.-) and a putative aldolase, respectively. The function of b0036 is supported by experimental evidence ( 11 ). VIMSS also assigns the same...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q9L6T2, Q93IM7), Crotonase family (P23966, P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had...”
- Genome annotation errors in pathway databases due to semantic ambiguity in partial EC numbers
Green, Nucleic acids research 2005 - “...in 10 KEGG pathway maps. In eight of these maps, two genes b0036 [Swiss-Prot entry P31551, Carnitinyl-CoA dehydratase (EC 4.2.1.-)] (example URL: ) and b1517 [Swiss-Prot entry P76143, Putative aldolase yneB (EC 4.2.1.-); hypothetical 31.9 kDa protein in hipBuxaB intergenic region] are both assigned to catalyze...”
PP_2217 enoyl-CoA hydratase/isomerase FadB1x from Pseudomonas putida KT2440
31% identity, 94% coverage
- β-oxidation-polyhydroxyalkanoates synthesis relationship in Pseudomonas putida KT2440 revisited
Liu, Applied microbiology and biotechnology 2023 - “...analysis, and using KT2440 PhaJ homologues for the search, other hydratases, such as PP_3726, PP_1845, PP_2217, PP_2136 and PP_3284, PP_3491, PP_3925 were identified. We have detected PP_1845, PP_2217, PP_2136, PP_3925 and PP_3491 in the proteome of both the WT and sextuple mutant, albeit without a statistically...”
- “...with or without nitrogen limitation; Supplemental information). However, there is a trend of increase in PP_2217 expression in the sextuple mutant compared to the WT, but due to the variation among biological replicates, this was not found to be a statistically significant change. Discussion It this...”
- Integration of ARTP Mutation and Adaptive Laboratory Evolution to Reveal 1,4-Butanediol Degradation in Pseudomonas putida KT2440
Qian, Microbiology spectrum 2023 - “...aldehyde dehydrogenase ( peaE ), oxidoreductase (PP_3208, PP_2737, fadBA ), acetyl-CoA C-acyltransferase (PP_2215), enoyl-CoA hydratase (PP_2217), acetyl-CoA C-acyltransferase ( fadA , PP_2215, pcaF-II ), and especially the enzymes in GCL pathway ( Fig.3A ). Hence, this pathway and enhances GCL module do improve the BDO degradation....”
- When metabolic prowess is too much of a good thing: how carbon catabolite repression and metabolic versatility impede production of esterified α,ω-diols in Pseudomonas putida KT2440
Lu, Biotechnology for biofuels 2021 - “...( FadA : acetyl-CoA acetyltransferase (PP_2137, PP_2215), FadB : 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (PP_2047, PP_2136, PP_2214, PP_2217), FadE : acyl-CoA dehydrogenase (PP_2048, PP_2216) [ 36 , 37 ] of -oxidation were deleted, generating P. putida BOX as a control strain. For alkyl acetate ester hydrolysis in P....”
- Genome-Wide Analysis of Targets for Post-Transcriptional Regulation by Rsm Proteins in Pseudomonas putida
Huertas-Rosales, Frontiers in molecular biosciences 2021 - “...Glutamine-hydrolyzing GMP synthase Purine metabolism PP_1073 glpD Aerobic glycerol-3-phosphate dehydrogenase PP_2080 gdhB NAD-specific glutamate dehydrogenase PP_2217 Enoyl-CoA hydratase PP_2437 Acyl-CoA dehydrogenase PP_2640 GNAT family acetyltransferase PP_2681 pqqD-II Pyrroloquinoline quinone biosynthesis chaperone PP_4571 cysK Cysteine synthase K Cysteine biosynthesis PP_5003 phaA poly (3-hydroxyalkanoate) polymerase Synthesis of carbon/energy...”
- Fatty Acid and Alcohol Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing
Thompson, Applied and environmental microbiology 2020 (secret) - Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin
Salvachúa, Microbial biotechnology 2020 - “...loci PP_22142217, where FadB is encoded at two separate coding regions PP_2214 (3hydroxyacylCoA dehydrogenase) and PP_2217 (enoylCoA hydratase) while FadA is encoded at PP_2215. This putative operon also encodes an acylCoA dehydrogenase ( fadE ; PP_2216). These two gene clusters, PP_21362137 and PP_22142217, were deleted in...”
c0045 carnitinyl-CoA dehydratase from Escherichia coli CFT073
32% identity, 95% coverage
BMEII1021 ENOYL-COA HYDRATASE from Brucella melitensis 16M
33% identity, 91% coverage
CD630_10570, CDIF630erm_01197 enoyl-CoA hydratase-related protein from Clostridioides difficile 630
CD1057 3-hydroxybutyryl-CoA dehydratase from Clostridium difficile 630
28% identity, 95% coverage
- The WalRK Two-Component System Is Essential for Proper Cell Envelope Biogenesis in Clostridioides difficile
Müh, Journal of bacteriology 2022 - “...flavoprotein beta 1.78 2.11 1.07 cd630_10560 10560 etfA Electron transfer flavoprotein alpha 1.95 2.18 1.10 cd630_10570 10570 crt2 3-Hydroxybutyryl-CoA dehydratase 1.96 2.27 1.06 cd630_31000 31000 C4-dicarboxylate anaerobic carrier 1.88 2.59 NC Membrane cd630_30990 30990 Amidohydrolase 1.80 2.47 NC Membrane cd630_10580 10580 hbd 3-Hydroxybutyryl-CoA dehydrogenase 1.86 2.05...”
- Iron Regulation in Clostridioides difficile
Berges, Frontiers in microbiology 2018 - “...OFF -7.70 OFF CD630_10560 CDIF630erm_01196 etfA Electron transfer flavoprotein subunit alpha -6.75 OFF -7.75 OFF CD630_10570 CDIF630erm_01197 crt2 3-hydroxybutyryl-CoA dehydratase -6.37 -7.48 OFF CD630_10580 CDIF630erm_01198 hbd 3-hydroxybutyryl-CoA dehydrogenase -5.21 OFF -5.97 OFF CD630_10590 CDIF630erm_01199 thlA1 Acetyl-CoA acetyltransferase -6.22 OFF -6.43 OFF CD630_29660 CDIF630erm_03250 adhE Bifunctional acetaldehyde-CoA/alcohol...”
- “...-7.70 OFF CD630_10560 CDIF630erm_01196 etfA Electron transfer flavoprotein subunit alpha -6.75 OFF -7.75 OFF CD630_10570 CDIF630erm_01197 crt2 3-hydroxybutyryl-CoA dehydratase -6.37 -7.48 OFF CD630_10580 CDIF630erm_01198 hbd 3-hydroxybutyryl-CoA dehydrogenase -5.21 OFF -5.97 OFF CD630_10590 CDIF630erm_01199 thlA1 Acetyl-CoA acetyltransferase -6.22 OFF -6.43 OFF CD630_29660 CDIF630erm_03250 adhE Bifunctional acetaldehyde-CoA/alcohol dehydrogenase...”
- Adaptive strategies and pathogenesis of Clostridium difficile from in vivo transcriptomics
Janoir, Infection and immunity 2013 - “...acetyltransferase; CD1058, 3-hydroxybutyryl-CoA-dehydrogenase; CD1057, 3-hydroxybutyryl-CoA dehydratase; CD1054, butyryl-CoA dehydrogenase; CD1055-CD1056,...”
- Effect of an oxygen-tolerant bifurcating butyryl coenzyme A dehydrogenase/electron-transferring flavoprotein complex from Clostridium difficile on butyrate production in Escherichia coli
Aboulnaga, Journal of bacteriology 2013 - “...(CD1056, etfA2), crotonase (EC 4.2.1.17) (CD1057, crt2), 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) (CD1058, hbd), and acetyl-CoA C-acetyltransferase...”
- Global transcriptional control by glucose and carbon regulator CcpA in Clostridium difficile
Antunes, Nucleic acids research 2012 - “...adhE (CD2966) and CD3006, aldehyde-alcohol dehydrogenase; thlA (CD1059), acetyl-CoA acetyltransferase; hbd (CD1058), 3-hydroxybutyryl-CoA dehydrogenase; crt2 (CD1057), 3-hydroxybutyryl-CoA dehydratase; cat1 (CD2343), succinyl-CoA: coenzyme A transferase; sucD (CD2342), succinate-semialdehyde dehydrogenase; 4hbd (CD2338), 4-hydroxybutyrate dehydrogenase; cat2 (CD2339), 4-hydroxybutyrate CoA transferase; abfD (CD2341), vinylacetyl-coa--isomerase; bcd2 (CD1054), butyryl-CoA dehydrogenase; etfBA (CD1055CD1056),...”
Saci_1109 3-hydroxybutyryl-CoA dehydrogenase from Sulfolobus acidocaldarius DSM 639
29% identity, 37% coverage
Psest_2437 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) from Pseudomonas stutzeri RCH2
32% identity, 92% coverage
- mutant phenotype: Specifically important for utilizing L-Isoleucine. Automated validation from mutant phenotype: the predicted function (METHYLACYLYLCOA-HYDROXY-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
FQU82_00193 enoyl-CoA hydratase from Acinetobacter baumannii
30% identity, 96% coverage
- Transcriptomic analysis reveals the regulatory role of quorum sensing in the Acinetobacter baumannii ATCC 19606 via RNA-seq
Xiong, BMC microbiology 2022 - “...the propanoate metabolism: FQU82_00191, FQU82_00562, FQU82_03635, FQU82_01642 ( paaF ), FQU82_00192, FQU82_00189 ( mmsA ), FQU82_00193, FQU82_00159 ( prpB ), FQU82_00160 ( prpC ), and FQU82_00161 ( acnD ). In purine metabolism, 8 DEGs were downregulated, including FQU82_00576 ( ndk ), FQU82_02944 ( purL ), FQU82_02508...”
Msed_0384 Enoyl-CoA hydratase/isomerase from Metallosphaera sedula DSM 5348
29% identity, 94% coverage
- Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Liu, mSphere 2021 - “...published data e Substrate, parameter Msed_2001 a , b Msed_0399 c , d Msed_0336 c Msed_0384 c Msed_0385 c Msed_0566 c 3-Hydroxypropionyl-CoA V max , U mg 1 protein 372 a /544 b 4.8 4 (Sp. act.) 4 (Sp. act.) ND ND K m , mM...”
- “...abundance can be ranked in the order Msed_2001 (set to 100%) > Msed_0399 (89%) > Msed_0384 (68%) > Msed_0385 (47%) > Msed_0566 (42%) > Msed_0336 (16%) ( Table3 ). The characterized crotonyl-CoA hydratase homologs (Msed_0399, Msed_0384, Msed_0336, and Msed_0566) display a V max too low to...”
- Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula
Liu, Frontiers in microbiology 2020 - “...Sulfolobales, strengthening this conclusion. Two uncharacterized crotonase homologs present in M. sedula genome (Msed_0336 and Msed_0384) were heterologously produced and characterized. Both proteins were highly efficient crotonyl-CoA hydratases and may contribute (or be responsible) for the corresponding reaction in the HP/HB cycle in vivo . 3-hydroxypropionate/4-hydroxybutyrate...”
- “...0.39 ND /2.2 f,h No Up 12.5 Hawkins et al., 2014 Crotonyl-CoA hydratase (14) Msed_0399 Msed_0384 Msed_0385 Msed_0336 Msed_0566 Msed_0399 15.0 13.8 f ,g /38 g /20 f,h ND ND ND ND No Down Down No No 19.7 Ramos-Vera et al., 2011 ; Hawkins et al.,...”
Rru_A3801 Enoyl-CoA hydratase/isomerase from Rhodospirillum rubrum ATCC 11170
30% identity, 95% coverage
Q1ZXF1 Probable enoyl-CoA hydratase, mitochondrial from Dictyostelium discoideum
27% identity, 92% coverage
- Genome-wide identification of pathogenicity factors of the free-living amoeba Naegleria fowleri
Zysset-Burri, BMC genomics 2014 - “...Tu Anaeromyxobacter sp. Fw109-5 2.46 2.42 Q9CR62 Mitochondrial 2-oxoglutarate/malate carrier protein Mus musculus 2.56 3.14 Q1ZXF1 Probable enoyl-CoA hydratase, mitochondrial Dictyostelium discoideum 2.69 4.44 F4P6T0 Ubiquinol oxidase, mitochondrial Batrachochytrium dendrobatidis JAM81 2.97 5.61 P77735 Uncharacterized oxidoreductase YajO Escherichia coli K-12 3.06 2.15 Q889U1 Single-stranded DNA-binding protein...”
O34893 Putative enoyl-CoA hydratase/isomerase YngF from Bacillus subtilis (strain 168)
BSU18220 enoyl-CoA hydratase from Bacillus subtilis subsp. subtilis str. 168
30% identity, 93% coverage
- Biodegradation of lignin monomers and bioconversion of ferulic acid to vanillic acid by Paraburkholderia aromaticivorans AR20-38 isolated from Alpine forest soil
Margesin, Applied microbiology and biotechnology 2021 - “...WP_069266866.1, WP_064271658.1, WP_012431551.1, WP_012431843.1, WP_035551717.1, WP_012432712.1, WP_035997457.1, WP_011489469.1, WP_011489931.1, WP_012434439.1, SDH36466.1, SDB90530.1, SAL69706.1, SAL32406.1, G4V4T7, O34893 Chromosome 1, 2, and 3 Feruloyl-SCoA to vanillin and acetyl-SCoA (Gasson et al. 1998 ) 5.0e-66; 1.0e-130; 1.6e-201; 1.7e-163; 1.1e-58; 3.5e-123; 2.6e-135; 7.1e-52; 0; 2.4e-130; 1.5e-132; 4.0e-138; 4.2e-157; 1.6e-139; 9.6e-143;...”
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q9HYA3, Q9HWK1, Q8ZQF0, Q8ZNE8, Q8ZR85, Q8ZL15, P40811, Q9L6T2, Q93IM7), Crotonase family (P23966, P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5,...”
- The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis
Eichenberger, PLoS biology 2004 - “...yknV (BSU14330), ykuD (BSU14040), ykvI (BSU13710), ykvU (BSU13830), ylbJ (BSU15030), ylbO/gerR (BSU15090), yngE (BSU18210), yngF (BSU18220), yngG (BSU18230), yngH (BSU18240), yngI (BSU18250), yngJ (BSU18260), yoaB (BSU18540), yoaD (BSU18560), yodU (BSU19810), ypqA (BSU22240), ypqP (BSU21670), yqfT (BSU25120), yqhV (BSU24440), yrdK (BSU26680), yydB (BSU40220), yydC (BSU40210), yydD (BSU40200),...”
swp_4934 Carnitine racemase from Shewanella piezotolerans WP3
32% identity, 97% coverage
VE01_00786 uncharacterized protein from Pseudogymnoascus verrucosus
30% identity, 85% coverage
- Geographical Diversity of Proteomic Responses to Cold Stress in the Fungal Genus Pseudogymnoascus
Abu, Microbial ecology 2023 - “...ribosomal protein S14 Pseudogymnoascus sp. 03VT05 79 12 150 16 10.87 2.8 1,352,887,002 Hypothetical protein VE01_00786 Pseudogymnoascus verrucosus 41 1 292 31.6 8.92 2.3 1,040,537,116 Argininosuccinate synthase Pseudogymnoascus sp. 05NY08 58 5 416 46.4 5.48 2.8 440,637,842 Phosphoglycerate kinase Pseudogymnoascus destructans 20,63121 62 1 417 44.4...”
WP_256691142 enoyl-CoA hydratase-related protein from Cetobacterium sp. NK01
27% identity, 95% coverage
C0PI72 Uncharacterized protein from Zea mays
34% identity, 62% coverage
acuH / Q5LWT8 acryloyl-CoA hydratase (EC 4.2.1.116) from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (see 2 papers)
SPO0147, SPO_RS00755 enoyl-CoA hydratase from Ruegeria pomeroyi DSS-3
27% identity, 96% coverage
- Oxidative Stress Regulates a Pivotal Metabolic Switch in Dimethylsulfoniopropionate Degradation by the Marine Bacterium Ruegeria pomeroyi
Wang, Microbiology spectrum 2022 - “...pomeroyi DSS-3. Genes: dmdA (SPO_RS09710), dmdB (SPO_RS10375, SPO_RS03420), dmdC (SPO_RS19300, SPO_RS01515, SPO_RS14785), dmdD (SPO_RS19305), acuH (SPO_RS00755), adlH (SPO_RS00490), mtoX (SPO_RS21180), dddP (SPO_RS11655), dddQ (SPO_RS22175), dddW (SPO_RS02290), prpE SPO_RS14880), and acuI (SPO_RS09715). RESULTS AND DISCUSSION Establishment of chemostat conditions. One goal of the planned studies was to...”
- Metabolism of dimethylsulphoniopropionate by Ruegeria pomeroyi DSS-3
Reisch, Molecular microbiology 2013 (PubMed)- “...also rapidly hydrated to 3-hydroxypropionyl-CoA by acryloyl-CoA hydratase (SPO0147). A SPO1914 mutant was unable to grow on acrylate as the sole carbon source,...”
- “...forming 3-hydroxypropionyl-CoA is catalysed by acryloyl-CoA hydratase (SPO0147). labelling patterns of amino acids and nucleosides following growth on [1-13C]...”
- Transcriptional changes underlying elemental stoichiometry shifts in a marine heterotrophic bacterium
Chan, Frontiers in microbiology 2012 - “...( norQ ) 2.2 0.1 SPOA0220 Cytochrome cd1 nitrite reductase ( nirS ) 0.2 P-LIMITED SPO0147 Enoyl-CoA hydratase 4.3 3.0 SPO0304 Lipoprotein, putative 7.0 5.4 SPO0468 Alkylphosphonate utilization protein ( phnG ) 5.2 SPO0472 Phosphonate C-P lyase system protein ( phnK ) 10.9 SPO0781 Phosphonate ABC...”
- “...transporter, periplasmic binding protein 6.6 SPOA0399 R body protein RebB-like protein 5.6 P-LIMITED (20 GENES) SPO0147 Enoyl-CoA hydratase 4.3 3.0 SPO0304 Putative lipoprotein 7.0 5.4 SPO0468 phnG Alkylphosphonate utilization protein 5.2 SPO0472 phnK Phosphonate C-P lyase system protein 10.9 SPO0505 rplO Ribosomal protein L15 3.2 5.7...”
Q8GB17 crotonobetainyl-CoA hydratase (EC 4.2.1.149) from Proteus sp. (see paper)
caiD / CAD48582.1 crotonobetainyl-CoA-hydratase from Proteus sp. LE138 (see paper)
33% identity, 94% coverage
Bd1852 hypothetical protein from Bdellovibrio bacteriovorus HD100
32% identity, 95% coverage
TGME49_317705 enoyl-CoA hydratase/isomerase family protein from Toxoplasma gondii ME49
30% identity, 67% coverage
- Identification of three novel Toxoplasma gondii rhoptry proteins
Camejo, International journal for parasitology 2014 - “...8 11.7 7.3 583.m05758 TGME49_316540 IMC sub-compartment protein ISP3 (ISP3) 1 0 11.1 8.0 611.m00052 TGME49_317705 enoyl-CoA hydratase/isomerase family protein 8 0 10.5 7.2 641.m01483 TGME49_318470 AP2 domain transcription factor AP2IV-4 (AP2IV4) 1 0 9.3 6.5 641.m00181 TGME49_320740 hypothetical protein 5 1 Yes 11.7 8.6 645.m00324...”
H16_B1905 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
32% identity, 93% coverage
TRIVIDRAFT_87842 uncharacterized protein from Trichoderma virens Gv29-8
27% identity, 82% coverage
- Transcriptome Dynamics Underlying Chlamydospore Formation in Trichoderma virens GV29-8
Peng, Frontiers in microbiology 2021 - “...differentially expressed ( Supplementary Table 23 ), including genes encoding acetyl-CoA C-acetyltransferase (TRIVIDRAFT_169943), enoyl-CoA hydratase (TRIVIDRAFT_87842), acetyl-CoA carboxylase (TRIVIDRAFT_78374), fatty acid synthase subunit beta, fungi type (TRIVIDRAFT_171412), fatty acid elongase 3 (TRIVIDRAFT_82162) and acetyl-CoA acyltransferase 1 (TRIVIDRAFT_80821). Genes related to lipid metabolism were differentially expressed in...”
- “...Figure 7B ). In these two modules, metabolic and oxidation-reduction genes were dominant. Enoyl-CoA hydratase (TRIVIDRAFT_87842), glucan endo-1,3-beta-D-glucosidase (TRIVIDRAFT_111476), glucanase (TRIVIDRAFT_89797) and phosphoglucomutase (TRIVIDRAFT_87728) were included ( Supplementary Table 16 , 17 ). The fatty acid degradation (tre00071) pathway was enriched in the dark red and...”
SMc01153 PROBABLE ENOYL COA HYDRATASE PROTEIN from Sinorhizobium meliloti 1021
31% identity, 95% coverage
couA / Q6N8W7 4-coumaroyl-CoA hydratase/aldolase from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) (see paper)
Q6N8W7 feruloyl-CoA hydratase/lyase (EC 4.1.2.61) from Rhodopseudomonas palustris (see paper)
RPA1786 putative 3-hydroxybutyryl-CoA dehydratase from Rhodopseudomonas palustris CGA009
32% identity, 79% coverage
- Anaerobic p-coumarate degradation by Rhodopseudomonas palustris and identification of CouR, a MarR repressor protein that binds p-coumaroyl coenzyme A
Hirakawa, Journal of bacteriology 2012 - “...R. palustris CGA009 CGA855 CGA856 CGA857 Wild-type couA (rpa1786) mutant couB (rpa1787) mutant couR (rpa1794) mutant; Kmr 19 This work This work This work...”
- “...plasmids by standard techniques. In-frame deletions of couA (rpa1786) and couB (rpa1787) were constructed by sequence overlap extension PCR (15) according to a...”
- Identification of a p-coumarate degradation regulon in Rhodopseudomonas palustris by Xpression, an integrated tool for prokaryotic RNA-seq data processing
Phattarasukol, Applied and environmental microbiology 2012 - “...mutant cells Wild type b Locus Size (bp) Kind of read rpa1786 rpa1786 rpa1786 rpa1786 297 297 807 807 Igbot Igtop Sense Antis a b Gene couA couR mutant Raw...”
- “...motif (GTTATA NNN TATAAC) (9) upstream of rpa1782 and rpa1786 and in the intergenic region between rpa1793 and couR (rpa1794). In addition, a CouR binding motif...”
- Environment sensing and response mediated by ABC transporters
Giuliani, BMC genomics 2011 - “...same strand as the SBP encode the enzymes p-coumaric acid-CoA ligase (RPA1787) and p-coumaroyl hydratase/lyase (RPA1786). These enzymes have been predicted to catalyze the first two catabolic steps of p-coumaric acid degradation [ 22 ]. Previously, microarray transcriptome profiling and quantitative proteomics measurements were performed with...”
- Anaerobic catabolism of aromatic compounds: a genetic and genomic view
Carmona, Microbiology and molecular biology reviews : MMBR 2009 - “...is located within a gene cluster that also contains RPA1786, a gene encoding a protein that is closely related to members of the enoyl-CoA hydratase/isomerase...”
- “...p-coumarate-CoA ligase (RPA1787), p-coumaroyl-CoA hydratase/lyase (RPA1786), and p-hydroxybenzaldehyde dehydrogenase (RPA1206). (B) Organization of the gene...”
AFUA_8G01210 enoyl-CoA hydratase/isomerase family protein from Aspergillus fumigatus Af293
31% identity, 91% coverage
A1S_1342 putative enoyl-CoA hydratase II from Acinetobacter baumannii ATCC 17978
30% identity, 86% coverage
- MacAB-TolC Contributes to the Development of Acinetobacter baumannii Biofilm at the Solid-Liquid Interface
Robin, Frontiers in microbiology 2021 - “...221.52 4.9E-03 ABYAL1577 A1S_1336 9.6 paaA 1,2-phenylacetyl-CoA epoxidase subunit A I 3 113.1 7.7E-04 ABYAL1583 A1S_1342 9.2 paaG 2-(1,2-epoxy-1.2-dihydrophenyl)acetyl-CoA isomerase I+M 8 394.61 3.1E-03 ABYAL1584 A1S_1343 9.6 paaH 3-hydroxybutyryl-CoA dehydrogenase I 3 178.95 2.6E-03 ABYAL1585 A1S_1344 12.4 paaJ Beta-ketoadipyl-CoA thiolase I 2 123.36 6.4E-03 Secretion system...”
- Mucin acts as a nutrient source and a signal for the differential expression of genes coding for cellular processes and virulence factors in Acinetobacter baumannii
Ohneck, PloS one 2018 - “...2.946424012 0.008872698 3-oxoadipate CoA-transferase subunit A, PcaI Phenylacetate catabolism A1S_1341 7.870503414 0.042039166 Enoyl-CoA hydratase, PaaF A1S_1342 8.861252528 0.039866679 Epoxyphenylacetyl-CoA isomerase, PaaG A1S_1343 10.85004468 0.023505204 3-hydroxyacyl-CoA dehydrogenase, PaaH A1S_1344 13.15884402 0.005538184 Beta-ketoadipyl CoA thiolase, PaaJ A1S_1345 * 15.00769651 0.001207025 Phenylacetate-CoA ligase, PaaK A1S_1346 * 14.78059953 0.00014185 Phenylacetate-CoA...”
- Effect of ethanol on differential protein production and expression of potential virulence functions in the opportunistic pathogen Acinetobacter baumannii
Nwugo, PloS one 2012 - “...Table 2 ): a putative enoyl-CoA hydratase II (spot 48, which relates to annotated gene A1S_1342), an acyl-CoA dehydrogenase (spot 51, which relates to annotated gene A1S_1376) and a thiolase (spot 56, which relates to annotated gene A1S_1344). Interestingly, the putative enoyl-CoA hydratase II and thiolase...”
GAH_01332 enoyl-CoA hydratase/isomerase family protein from Geoglobus ahangari
28% identity, 95% coverage
RPPS3_06600 enoyl-CoA hydratase-related protein from Rhodopseudomonas palustris
31% identity, 96% coverage
bhn_I2225 enoyl-CoA hydratase-related protein from Butyrivibrio hungatei
31% identity, 94% coverage
- The complete genome sequence of the rumen bacterium Butyrivibrio hungatei MB2003
Palevich, Standards in genomic sciences 2017 - “...pyruvate conversion to acetyl-CoA, as well as a butyryl-CoA dehydrogenase/electron transferring flavoprotein bcd - etfAB (bhn_I2225, bhn_I2221 and bhn_I2222) to generate ATP by classic substrate level phosphorylation. In addition, MB2003 possesses genes that encode all six subunits of the Rnf ( rnfA , rnfB , rnfC...”
MELS_1449 short-chain-enoyl-CoA hydratase from Megasphaera elsdenii DSM 20460
28% identity, 92% coverage
WP_027131966 enoyl-CoA hydratase-related protein from Fusobacterium necrophorum HUN048
27% identity, 94% coverage
Dred_0401 Enoyl-CoA hydratase/isomerase from Desulfotomaculum reducens MI-1
28% identity, 95% coverage
Cspa_c04330 short-chain-enoyl-CoA hydratase from Clostridium saccharoperbutylacetonicum N1-4(HMT)
29% identity, 94% coverage
A1S_1341 Enoyl-CoA hydratase/carnithine racemase from Acinetobacter baumannii ATCC 17978
34% identity, 62% coverage
- Insights Into Mechanisms of Biofilm Formation in Acinetobacter baumannii and Implications for Uropathogenesis
Colquhoun, Frontiers in cellular and infection microbiology 2020 - “...14.96 A132, ATCC 17978 Nait Chabane et al., 2014 ; Kentache et al., 2017 paaF A1S_1341 enoyl-CoA hydratase/carnithine racemase 161.43 ATCC 17978 Rumbo-Feal et al., 2013 2.46 ATCC 17978 Kentache et al., 2017 paaJ A1S_1344 beta-ketoadipyl CoA thiolase 28.43 ATCC 17978 Rumbo-Feal et al., 2013 16.96...”
- Colistin Resistant A. baumannii: Genomic and Transcriptomic Traits Acquired Under Colistin Therapy
Cafiso, Frontiers in microbiology 2018 - “...ATCC 17978 showed 3 affected KEGG pathways including the Butanoate metabolism (5 genes: A1S_1729, A1S_1699, A1S_1341, A1S_2102, A1S_1705), Tryptophan metabolism (4 genes: A1S_1729, A1S_1341, A1S_2102, A1S_2450), Lysine degradation (3 genes: A1S_1729, A1S_1341, A1S_2102), Fatty acid metabolism, Limonene and pinene degradation (3 genes: A1S_1344, A1S_1341, A1S_2102), Benzoate...”
- Mucin acts as a nutrient source and a signal for the differential expression of genes coding for cellular processes and virulence factors in Acinetobacter baumannii
Ohneck, PloS one 2018 - “...0.000100525 Beta-ketoadipyl CoA thiolase, PcaF A1S_1894 2.946424012 0.008872698 3-oxoadipate CoA-transferase subunit A, PcaI Phenylacetate catabolism A1S_1341 7.870503414 0.042039166 Enoyl-CoA hydratase, PaaF A1S_1342 8.861252528 0.039866679 Epoxyphenylacetyl-CoA isomerase, PaaG A1S_1343 10.85004468 0.023505204 3-hydroxyacyl-CoA dehydrogenase, PaaH A1S_1344 13.15884402 0.005538184 Beta-ketoadipyl CoA thiolase, PaaJ A1S_1345 * 15.00769651 0.001207025 Phenylacetate-CoA ligase,...”
- Whole transcriptome analysis of Acinetobacter baumannii assessed by RNA-sequencing reveals different mRNA expression profiles in biofilm compared to planktonic cells
Rumbo-Feal, PloS one 2013 - “...0.41 A1S_1339 phenylacetate-CoA oxygenase PaaJ subunit 196.37 0.78 A1S_1340 phenylacetate-CoA oxygenase/reductase PaaK subunit 161.34 0.77 A1S_1341 enoyl-CoA hydratase/carnithine racemase 28.43 0.56 A1S_1344 thiolase 14.31 0.40 A1S_1357 alanine racemase 4.59 1.23 A1S_1370 oxidoreductase 2.67 0.80 A1S_1376 acyl-CoA dehydrogenase 11.34 3.14 A1S_1377 acrR family transcriptional regulator 4.28 0.56...”
CCNA_00006 enoyl-CoA hydratase from Caulobacter crescentus NA1000
CC0006 enoyl-CoA hydratase/isomerase family protein from Caulobacter crescentus CB15
33% identity, 92% coverage
A0A0H3M485 Probable enoyl-CoA hydratase echA5 from Mycobacterium bovis (strain BCG / Pasteur 1173P2)
Rv0675 enoyl-CoA hydratase from Mycobacterium tuberculosis H37Rv
YP_177745 enoyl-CoA hydratase EchA5 from Mycobacterium tuberculosis H37Rv
36% identity, 69% coverage
- Identification of antigens presented by MHC for vaccines against tuberculosis
Bettencourt, NPJ vaccines 2020 - “...mbtD A0A0H3M738 dnaB A0A0H3M178 metH A0A0H3M6G7 dnaN_1 A0A0G2Q9D4 mmpL12 A0A0H3M689 dxs A1KM20 mmpL14 A0A0H3M5L6 echA5 A0A0H3M485 mmpL3 A0A0H3M2S6 efpA A0A0H3MDJ5 PE_PGRS14 A0A0H3M376 embA A0A0H3MFP8 PE_PGRS27 A0A0H3M617 embB A0A0H3MC15 pks12 A0A0H3M7L7 eno A1KHG1 PPE24 A0A0H3M5L1 fadD26 A0A0H3M8A6 PPE4 A0A0H3MA86 fadE34 A0A0H3MIH3 PPE8 A0A0H3M207 fas A0A0H3MFR6 ppsD A0A0H3MGQ7...”
- Immunogenicity of Mycobacterial Extracellular Vesicles Isolated From Host-Related Conditions Informs About Tuberculosis Disease Status
Schirmer, Frontiers in microbiology 2022 - “...Rv2831 EchA16 0.59 Rv2244 AcpM 1.2 Rv0824c DesA1 0.56 Rv2831 EchA16 1.08 Rv3800c Pks13 0.54 Rv0675 EchA5 1.07 Rv1070c EchA8 0.51 Rv0642c MmaA4 0.72 Information pathways 93 Rv0723 RpIO 1.18 41 Rv0054 Ssb 1.73 Rv0054 Ssb 1.06 Rv0716 RpIE 1.23 Rv2904c RpIS 1 Rv0714 RpIN 1.21...”
- Lineage-Specific Proteomic Signatures in the Mycobacterium tuberculosis Complex Reveal Differential Abundance of Proteins Involved in Virulence, DNA Repair, CRISPR-Cas, Bioenergetics and Lipid Metabolism
Yimer, Frontiers in microbiology 2020 - “...4, 5 Membrane-anchored mycosin MycP mycp3 Rv0291 7 3, 4, 5 Siderophore exporter MmpL5 mmpl5 Rv0675 7 3, 4, 5 TABLE 2 Differentially abundant proteins in the ESX-1 secretion system and other virulence related proteins in MTBC lineages. Protein name Gene symbol Rv number Less abundance...”
- Mycobacteriophage SWU1 gp39 can potentiate multiple antibiotics against Mycobacterium via altering the cell wall permeability
Li, Scientific reports 2016 - “...MSMEG_1387 3.64 Rv0672 involved in lipid degradation MSMEG_1388 4.16 Rv0673 enoyl-CoA hydratase EchA4 MSMEG_1390 3.39 Rv0675 enoyl-CoA hydratase EchA5 MSMEG_5996 3.24 Rv3546 involved in lipid degradation MSMEG_4717 3.00 Rv2502c fatty acid metabolism MSMEG_6041 2.41 Rv3573c lipid degradation MSMEG_3465 2.18 Rv1925 lipid degradation MSMEG_4716 2.51 Rv2501c lipid...”
- Proteome analysis of the Mycobacterium tuberculosis Beijing B0/W148 cluster
Bespyatykh, Scientific reports 2016 - “...metabolism and respiration 0.86 0.0386941 Rv0243 under Rv0238 acetyl-CoA acetyltransferase FadA Lipid metabolism 0.91 0.0166606 Rv0675 under Rv0674 enoyl-CoA hydratase EchA5 Lipid metabolism 1.1 0.0007597 Rv0768 under Rv0576; Rv1255c aldehyde dehydrogenase AldA Intermediary metabolism and respiration Rv0824c hycD under Rv0472c acyl-ACP desaturase DesA Lipid metabolism 1.98...”
- The Mycobacterium tuberculosis β-oxidation genes echA5 and fadB3 are dispensable for growth in vitro and in vivo.
Williams, Tuberculosis (Edinburgh, Scotland) 2011 - GeneRIF: gene echA5 is dispensable for growth in vitro and in vivo
CPE0095 crotonase from Clostridium perfringens str. 13
29% identity, 91% coverage
H16_A3307 putative enoyl-CoA hydratase from Ralstonia eutropha H16
H16_A3307 enoyl-CoA hydratase from Cupriavidus necator H16
32% identity, 95% coverage
- Microaerobic insights into production of polyhydroxyalkanoates containing 3-hydroxyhexanoate via native reverse β-oxidation from glucose in Ralstonia eutropha H16
Huong, Microbial cell factories 2024 - “...dehydrogenases PaaH1 (H16_A0282) and Had (H16_A0602), as well as the ( S )-specific crotonase Crt2 (H16_A3307) in R. eutropha were reported to be broad substrate specific [ 20 ], thus possess capability to function in conversion of 3-oxoacyl-CoA to trans -2-enoyl-CoA via ( S )-3-hydroxyacyl-CoA of...”
- “...), phaB3 ( h16_A2171 ), had ( h16_A0602 ), paaH1 ( h16_A0282 ), crt2 ( h16_A3307 ), bktB ( h16_A1445 ), phaJ4a ( h16_A1070 ), h16_A3330 , and fadB ( h16_A0461 ) (Additional file 1 : Table S2). The deletion vectors for bktB and h16_A3330 were...”
- Biosynthesis of Polyhydroxyalkanoate Terpolymer from Methanol via the Reverse β-Oxidation Pathway in the Presence of Lanthanide
Orita, Microorganisms 2022 - “...at the corresponding site to obtain pCM80Km_emdbktB. A tandem of had Re (H16_A0602)- crt2 Re (H16_A3307) genes was prepared by fusion PCR. The had Re and crt2 Re fragments were individually amplified from R. eutropha genomic DNA using A0602-F/A0602-R-Fus and A3307-F-Fus/A3307-R as the primer sets, respectively....”
- Insights into the Degradation of Medium-Chain-Length Dicarboxylic Acids in Cupriavidus necator H16 Reveal β-Oxidation Differences between Dicarboxylic Acids and Fatty Acids
Strittmatter, Applied and environmental microbiology 2022 (secret) - Two NADH-dependent (S)-3-hydroxyacyl-CoA dehydrogenases from polyhydroxyalkanoate-producing Ralstonia eutropha
Segawa, Journal of bioscience and bioengineering 2019 (PubMed)- “...partially purified and identified as H16_A3307. 0 false false Polyhydroxyalkanoates Poly(3-hydroxybutyrate) Ralstonia eutropha NADH-3-hydroxyacyl-CoA...”
- “...partially purified and identified as H16_A3307. Key words Polyhydroxyalkanoates Poly(3-hydroxybutyrate) Ralstonia eutropha NADH-3-hydroxyacyl-CoA dehydrogenase...”
- Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds
Zhang, Microbial cell factories 2019 - “...PaaH1 (H16_A0282) and Had (H16_A0602), as well as ( S )-specific enoyl-CoA hydratase (crotonase) Crt2 (H16_A3307) in the cell extract of R. eutropha [ 39 ]. Analysis of the enzymatic characteristics indicated that these enzymes showed rather broad specificities with high activities toward the C 4...”
- Whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in Ralstonia eutropha H16
Brigham, Applied and environmental microbiology 2012 - “...reductase), fabZ (encoding 3-hydroxymyristoyl-[ACP] dehydratase), and H16_A3307 (encoding a putative enoyl-CoA hydratase). Our microarray data have confirmed...”
- Genome-wide transcriptome analyses of the 'Knallgas' bacterium Ralstonia eutropha H16 with regard to polyhydroxyalkanoate metabolism
Peplinski, Microbiology (Reading, England) 2010 (PubMed)- “...In strain H16, phaP3, accC2, fabZ, fabG and H16_A3307 exhibited a decreased transcription level in the stationary growth phase compared with the transition...”
- “...Compared with PHB"4, we found that phaA, phaB1, paaH1, H16_A3307, phaP3, accC2 and fabG were induced in the wildtype, and phaP1, phaP4, phaZ2 and phaZ6...”
WP_009006293 enoyl-CoA hydratase-related protein from Fusobacterium necrophorum D12
27% identity, 94% coverage
FP2_20590 enoyl-CoA hydratase-related protein from Faecalibacterium prausnitzii L2-6
29% identity, 94% coverage
A9762_22630 crotonase/enoyl-CoA hydratase family protein from Pandoraea sp. ISTKB
33% identity, 87% coverage
ECHM_MOUSE / Q8BH95 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Mus musculus (Mouse) (see paper)
NP_444349 enoyl-CoA hydratase, mitochondrial precursor from Mus musculus
29% identity, 84% coverage
- function: Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl- CoA) to the corresponding (3S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (By similarity). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)- butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta- oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)- hydroxyhexanoyl-CoA) (By similarity).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA (RHEA:45228)
catalytic activity: (3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA (RHEA:45736)
catalytic activity: (3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:26558)
catalytic activity: 3-hydroxyisovaleryl-CoA = 3-methyl-(2E)-butenoyl-CoA + H2O (RHEA:31079)
catalytic activity: 3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O (RHEA:26518)
catalytic activity: 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:45584)
catalytic activity: (2E)-2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl- CoA (RHEA:31175)
catalytic activity: (3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O (RHEA:30547)
catalytic activity: (3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O (RHEA:31191)
subunit: Homohexamer; dimer of trimers. - Branched-Chain Amino Acid Degradation Pathway was Inactivated in Colorectal Cancer: Results from a Proteomics Study
Lian, Journal of Cancer 2024 - “...Yes Q61425 Hadh Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 21.34 7 1.01 0.53 0.51 Down 0.25 Yes Q8BH95 Echs1 Enoyl-CoA hydratase, mitochondrial 35.52 12 1 0.52 0.48 Down 0.99 Yes Q8QZT1 Acat1 Acetyl-CoA acetyltransferase, mitochondrial 17.45 9 1 0.63 0.62 Down 0.91 Yes Q9CZS1 Aldh1b1 Aldehyde dehydrogenase X,...”
- Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular Stressors.
Starr, bioRxiv : the preprint server for biology 2024 - “...Kras protein Q5J7N1 0.027 reduced AP-1 complex subunit beta-1 O35643 0.005 reduced Enoyl-CoA hydratase, mitochondrial Q8BH95 0.045 reduced Transforming protein RhoA Q9QUI0 0.037 reduced Prohibitin P67778 0.044 reduced Transportin-1 Q8BFY9 0.040 reduced 26S proteasome non-ATPase regulatory subunit 2 Q8VDM4 0.023 reduced Tubby-related protein 1 Q9Z273 0.042...”
- Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis
Banfi, Antioxidants (Basel, Switzerland) 2023 - “...P97807 6 0.004 1.79 NEG Fumarate hydratase_ mitochondrial OS = Mus musculus GN = Fh Q8BH95 3 0.037 1.70 NEG Enoyl-CoA hydratase_ mitochondrial OS = Mus musculus GN = Echs1 Q9WTP7 2 0.007 1.68 NEG GTP:AMP phosphotransferase AK3_ mitochondrial OS = Mus musculus GN = Ak3...”
- ISG15 Is a Novel Regulator of Lipid Metabolism during Vaccinia Virus Infection.
Albert, Microbiology spectrum 2022 - “...Peroxisomal acyl-coenzyme A oxidase 3 8.97 P97742 Cpt1a Carnitine O -palmitoyltransferase 1, liver isoform 8.54 Q8BH95 Echs1 Enoyl-CoA hydratase, mitochondrial 8.31 Q07417 Acads Short-chain-specific acyl-CoA dehydrogenase, mitochondrial 7.62 P51174 Acadl Long-chain-specific acyl-CoA dehydrogenase, mitochondrial 6.74 Q8QZT1 Acat1 Acetyl-CoA acetyltransferase, mitochondrial 6.14 P42125 Eci1 Enoyl-CoA delta isomerase...”
- “...mitochondrial 7.71 Q9Z2Z6 Slc25a20 Mitochondrial carnitine/acylcarnitine carrier protein 6.67 Q9JHI5 Ivd Isovaleryl-CoA dehydrogenase, mitochondrial 6.39 Q8BH95 Echs1 Enoyl-CoA hydratase, mitochondrial 5.58 Q8JZN5 Acad9 Complex I assembly factor ACAD9, mitochondrial 5.39 Q99JY0 Hadhb Trifunctional enzyme subunit beta, mitochondrial 5.11 Q9CQ62 Decr1 2,4-Dienoyl-CoA reductase ([3E]-enoyl-CoA-producing), mitochondrial 4.23 P50544...”
- Proteomic Analysis of Mucopolysaccharidosis IIIB Mouse Brain
De, Biomolecules 2020 - “...M Q3TYV5 Cnp 2,3-cyclic-nucleotide 3-phosphodiesterase 0.5 0.00001 ERS B7U582 Heat shock protein 70-2 0.5 0.00001 Q8BH95 Echs1 Enoyl-CoA hydratase, mitochondrial 0.5 0.03689 M Q6P1J1 Crmp1 Crmp1 protein 0.5 0.00045 CK, C, N B2CY77 Rpsa Laminin receptor (Fragment) 0.5 0.01872 C, ERS, N, PM Q922F4 Tubb6 Tubulin...”
- PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.
Feng, International journal of oncology 2020 - “...Peroxisomal bifunctional enzyme 1.71 Up 0.0032 Q61425 Hadh Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 1.69 Up 0.0012 Q8BH95 Echs1 Enoyl-CoA hydratase, mitochondrial 1.69 Up 0.0418 P51174 Acadl Long-chain specific acyl-CoA dehydrogenase, mitochondrial 1.64 Up 0.0386 Q921H8 Acaa1a 3-ketoacyl-CoA thiolase A, peroxisomal 1.57 Up 0.0249 Q9R0H0 Acox1 Peroxisomal acyl-coenzyme...”
- γ-Oryzanol Improves Cognitive Function and Modulates Hippocampal Proteome in Mice
Rungratanawanich, Nutrients 2019 - “...0.001 1.539 Q9CR68 Cytochrome b-c1 complex subunit Rieske, mitochondrial (Uqcrfs1) 19 29.3 8.70 0.000 2.014 Q8BH95 Enoyl-CoA hydratase, mitochondrial (Echs1) 30 31.5 8.48 0.001 1.226 P08226 Apolipoprotein E (ApoE) 19 35.8 5.68 0.000 2.826 P05064 Fructose-bisphosphate aldolase A (Aldoa) 61 39.3 8.09 0.001 0.413 P35486 Pyruvate...”
- Thymic Microenvironment Is Modified by Malnutrition and Leishmania infantum Infection
Losada-Barragán, Frontiers in cellular and infection microbiology 2019 - “...Up P56391 10046.87 3 16 0.779 2.2 0.003 Cytochrome c oxidase subunit 6B1 Cox6b1 Up Q8BH95 31436.2 4 21 1.269 3.6 0.001 Enoyl-CoA hydratase, mitochondrial Echs1 Up P08249 35570.75 4 22 0.697 2.0 0.001 Malate dehydrogenase, mitochondrial Mdh2 Up Q99KI0 85392.01 7 33 0.785 2.2 0.000...”
- More
- Type 2 diabetic obese db/db mice are refractory to myocardial ischaemic post-conditioning in vivo: potential role for Hsp20, F1-ATPase δ and Echs1.
Zhu, Journal of cellular and molecular medicine 2012 - GeneRIF: potential protein targets for the loss of PostC may include F(1)-ATPase gamma, Echs1 and Hsp20 that could regulate cellular ATP consumption/production and defense response to ischaemic stress
Msed_0399 / A4YDS4 crotonyl-CoA hydratase/(S)-3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.35; EC 4.2.1.150) from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) (see 3 papers)
A4YDS4 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Metallosphaera sedula (see 2 papers)
Msed_0399 3-hydroxyacyl-CoA dehydrogenase, NAD-binding from Metallosphaera sedula DSM 5348
29% identity, 37% coverage
- Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Liu, mSphere 2021 - “...well as crotonyl-CoA hydratase, and (ii) a bifunctional fusion protein crotonyl-CoA hydratase/( S )-3-hydroxybutyryl-CoA dehydrogenase Msed_0399; they are both present in, and were purified from, autotrophically grown cells ( 15 , 16 ). In M. sedula cell extracts, correspondingly, the crotonyl-CoA hydratase activity is much higher...”
- “...universally found in autotrophic Sulfolobales ) and that the bifunctional crotonyl-CoA hydratase/( S )-3-hydroxybutyryl-CoA dehydrogenase Msed_0399 is apparently not the main crotonyl-CoA hydratase in this archaeon, although this protein is present in autotrophically grown cells ( 17 ). Indeed, the genome contains four more dehydratase candidates...”
- (S)-3-Hydroxybutyryl-CoA Dehydrogenase From the Autotrophic 3-Hydroxypropionate/4-Hydroxybutyrate Cycle in Nitrosopumilus maritimus
Liu, Frontiers in microbiology 2021 - “...low ( S )-3-hydroxybutyryl-CoA dehydrogenase activity. The activity of bifunctional crotonyl-CoA hydratase/( S )-3-hydroxybutyryl-CoA dehydrogenase Msed_0399 is comparable to that of Nmar_1028, while its K m value is 6 times higher than that of Nmar_1028 ( Tables 1 , 3 ). Msed_1423 is the main (...”
- “...). The catalytic properties of Nmar_1028 are rather comparable with those of Msed_1423 than of Msed_0399. Indeed, the k cat / K m values of Nmar_1028 and Msed_1423 were 5- and 20-fold higher than the corresponding value of Msed_0399 in the ( S )-3-hydroxybutyryl-CoA dehydrogenase reaction...”
- Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula
Liu, Frontiers in microbiology 2020 - “...model autotrophic member of Sulfolobales, Metallosphaera sedula , possesses in addition to the bifunctional protein (Msed_0399) several separate genes coding for crotonyl-CoA hydratase and ( S )-3-hydroxybutyryl-CoA dehydrogenase. Their genes were previously shown to be transcribed under autotrophic and mixotrophic conditions. The dehydrogenase Msed_1423 (and not...”
- “...Msed_1321 0.39 ND /2.2 f,h No Up 12.5 Hawkins et al., 2014 Crotonyl-CoA hydratase (14) Msed_0399 Msed_0384 Msed_0385 Msed_0336 Msed_0566 Msed_0399 15.0 13.8 f ,g /38 g /20 f,h ND ND ND ND No Down Down No No 19.7 Ramos-Vera et al., 2011 ; Hawkins et...”
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate CO2 fixation cycle in extremely thermoacidophilic archaea
Loder, Metabolic engineering 2016 - “...H 2 O R ( Hawkins et al., 2014 ) Crotonyl-CoA hydratase/(S)-3-Hydroxybutyryl-CoA dehydrogenase (NADH) CCH/HBCD Msed_0399 a) Crotonyl-CoA + H 2 O ( S )-3-Hydroxybutyryl-CoA b) ( S )-3-Hydroxybutyryl-CoA + NAD Acetoacetyl-CoA + NADH NP ( Ramos-Vera et al., 2011 ), R ( Hawkins et al.,...”
- Novel Transcriptional Regulons for Autotrophic Cycle Genes in Crenarchaeota
Leyn, Journal of bacteriology 2015 - “...(hpcD-hbd) from the HHC pathway, which is encoded by Msed_0399 in M. sedula (6), does not belong to the reconstructed HhcR regulon in the Sulfolobales spp. (see...”
- “...M. sedula), which is 46% identical to Msed_0399. Interestingly, the Sulfolobales have at least three 4-hydroxybutyryl-CoA synthetase gene candidates. Msed_0406...”
- Conversion of 4-hydroxybutyrate to acetyl coenzyme A and its anapleurosis in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway
Hawkins, Applied and environmental microbiology 2014 - “...(Msed_1321), crotonyl-CoA hydratase/(S)-3-hydroxybutyrylCoA dehydrogenase (Msed_0399), and acetoacetyl-CoA -ketothiolase (Msed_0656), were produced...”
- “...Msed_2001 Msed_1426 Msed_0639 Msed_0638, Msed_2055 Msed_1424 Msed_0406 Msed_1321 Msed_0399 Msed_0656 NCE (6, 34) R (35, 36) R (36) NP (37) NP, R (38) NP...”
- Role of 4-hydroxybutyrate-CoA synthetase in the CO2 fixation cycle in thermoacidophilic archaea
Hawkins, The Journal of biological chemistry 2013 - “...Msed_0638 Msed_2055 Msed_1424 Msed_0394 Msed_0406 Msed_1321 Msed_0399 Msed_0656 CO2-H2 Autotrophy in Metallosphaera sedula FIGURE 2. Bioreactor schematic for...”
- Epimerase (Msed_0639) and mutase (Msed_0638 and Msed_2055) convert (S)-methylmalonyl-coenzyme A (CoA) to succinyl-CoA in the Metallosphaera sedula 3-hydroxypropionate/4-hydroxybutyrate cycle
Han, Applied and environmental microbiology 2012 - “...HBCD CCH Msed_1424 Unknown Msed_1321 Msed_0399 16 ACK Msed_0656 Malonyl-CoA/succinyl-CoA reductase Malonate semialdehyde reductase 3-Hydroxypropionyl-CoA...”
- More
AUO97_RS06660 enoyl-CoA hydratase from Acinetobacter baumannii
30% identity, 96% coverage
- The abaI/abaR Quorum Sensing System Effects on Pathogenicity in Acinetobacter baumannii
Sun, Frontiers in microbiology 2021 - “...abaIR mutant enhances slightly more virulent than abaI . In the abaI mutant, AUO97_RS14195, AUO97_RS16430, AUO97_RS06660, AUO97_RS18630, and AUO97_RS12705 involved in the propanoate metabolism pathway were upregulated. In the abaIR mutant, AUO97_RS14380, AUO97_RS14375, and AUO97_RS14370 involved in the propanoate metabolism pathway were upregulated. Lipids play an...”
ScpB / b2919 methylmalonyl-CoA decarboxylase from Escherichia coli K-12 substr. MG1655 (see 5 papers)
scpB / P52045 methylmalonyl-CoA decarboxylase from Escherichia coli (strain K12) (see 4 papers)
SCPB_ECOLI / P52045 Methylmalonyl-CoA decarboxylase; MMCD; Transcarboxylase; EC 4.1.1.- from Escherichia coli (strain K12) (see 2 papers)
1ef9A / P52045 The crystal structure of methylmalonyl coa decarboxylase complexed with 2s-carboxypropyl coa (see paper)
b2919 putative enzyme from Escherichia coli str. K-12 substr. MG1655
29% identity, 92% coverage
- function: Catalyzes the decarboxylation of (R)-methylmalonyl-CoA to propionyl-CoA. Could be part of a pathway that converts succinate to propanoate.
catalytic activity: (R)-methylmalonyl-CoA + H(+) = propanoyl-CoA + CO2 (RHEA:27666)
subunit: Dimer of homotrimers. - Ligand: 2-carboxypropyl-coenzyme a (1ef9A)
- Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q93IM7), Crotonase family (P23966, P40805, P40802, O07533, O34893, P94549, O32178, P0ABU0, P76082, P21177, P77399, P31551, P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment...”
- luxS-dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling
Wang, Journal of bacteriology 2005 - “...b0076 b2723 b3683 b3028 b2968 b0579 b3220 b0260 b3046 b2919 b3906 b2060 b0790 b1720 b1010 b2797 b2993 b0042 b1001 b2549 b1311 b3141 b1012 b1571 lsrG lsrD lsrA...”
CRCH_SYNWW / Q0AVM1 Crotonyl-CoA hydratase; EC 4.2.1.150 from Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) (see paper)
Swol_1936 3-hydroxybutyryl-CoA dehydratase from Syntrophomonas wolfei subsp. wolfei str. Goettingen
28% identity, 95% coverage
- function: Involved in syntrophic growth of S.wolfei with butyrate, as part of the butyrate oxidation pathway. Probably catalyzes the hydration of crotonyl-CoA to 3-hydroxybutyryl-CoA.
catalytic activity: 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:45584)
catalytic activity: a short-chain (3S)-3-hydroxyacyl-CoA = a short-chain (2E)- enoyl-CoA + H2O (RHEA:52664)
subunit: Homotetramer. - Proteomic analysis reveals metabolic and regulatory systems involved in the syntrophic and axenic lifestyle of Syntrophomonas wolfei
Sieber, Frontiers in microbiology 2015 - “...interspecies interactions (Supplemental Table 2 ). Two proteins have annotated functions in beta-oxidation (Swol_1935 and Swol_1936 gene products) and one has an annotated function in poly-(3-hydroxyalkanoate) synthesis [poly-(3-hydroxyalkanoic acid) synthase, Swol_1241 gene product]. Two proteins with unknown function (Swol_1036 and Swol_2364 gene products) were detected. Swol_1036...”
- A proteomic view at the biochemistry of syntrophic butyrate oxidation in Syntrophomonas wolfei
Schmidt, PloS one 2013 - “...that is attributed to act as EtfAB:quinone oxidoreductase (see Discussion ). The crotonyl-CoA hydratase gene Swol_1936 in the gene cluster Swol_1933-36 was identified by the prominent spot E14 ( Fig. 2AB ), and the NAD-dependent 3-hydroxybutyryl-CoA dehydrogenase gene Swol_1935 by two prominent protein spots, E18 and...”
- Involvement of NADH:acceptor oxidoreductase and butyryl coenzyme A dehydrogenase in reversed electron transport during syntrophic butyrate oxidation by Syntrophomonas wolfei
Müller, Journal of bacteriology 2009 - “...not shown), i.e., enoylCoA hydratase (Swol_1936), 3-hydroxyacyl-CoA dehydrogenase (Swol_1935), and acetyl-CoA C-acyltransferases (Swol_ 1934 and Swol_2051)....”
Q9NEZ8 Enoyl-CoA hydratase from Caenorhabditis elegans
30% identity, 91% coverage
badK / O07453 BadK from Rhodopseudomonas palustris (see paper)
RPA0650 cyclohex-1-ene-1-carboxyl-CoA hydratase from Rhodopseudomonas palustris CGA009
30% identity, 96% coverage
H16_A0179 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
H16_A0179 enoyl-CoA hydratase/isomerase family protein from Cupriavidus necator H16
32% identity, 93% coverage
RPYSC3_06760 enoyl-CoA hydratase-related protein from Rhodopseudomonas palustris
30% identity, 96% coverage
5jbxB / Q1D5Y4 Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
31% identity, 95% coverage
- Ligand: coenzyme a (5jbxB)
Q1D5Y4 methylglutaconyl-CoA hydratase (EC 4.2.1.18) from Myxococcus xanthus (see paper)
31% identity, 95% coverage
3h81A / P9WNN9 Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
29% identity, 96% coverage
- Ligand: calcium ion (3h81A)
BAB2_1046 Enoyl-CoA hydratase/isomerase from Brucella melitensis biovar Abortus 2308
33% identity, 92% coverage
Rv1070c enoyl-CoA hydratase from Mycobacterium tuberculosis H37Rv
29% identity, 96% coverage
- Revolutionizing control strategies against Mycobacterium tuberculosis infection through selected targeting of lipid metabolism
Kim, Cellular and molecular life sciences : CMLS 2023 - “...H, Capreomycin drrA, drrB, drrC rv2936-2938 VAN Down echA8 rv1070c H, Capreomycin Up echA12 rv1472 H Up fabD rv2243 H, Thiolactomycin Up R Down fadD2 rv0270 H...”
- Immunogenicity of Mycobacterial Extracellular Vesicles Isolated From Host-Related Conditions Informs About Tuberculosis Disease Status
Schirmer, Frontiers in microbiology 2022 - “...Rv2244 AcpM 1.2 Rv0824c DesA1 0.56 Rv2831 EchA16 1.08 Rv3800c Pks13 0.54 Rv0675 EchA5 1.07 Rv1070c EchA8 0.51 Rv0642c MmaA4 0.72 Information pathways 93 Rv0723 RpIO 1.18 41 Rv0054 Ssb 1.73 Rv0054 Ssb 1.06 Rv0716 RpIE 1.23 Rv2904c RpIS 1 Rv0714 RpIN 1.21 Rv0714 RpIN 0.86...”
- Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-starvation-responsive toxin-antitoxin systems
Albrethsen, Molecular & cellular proteomics : MCP 2013 - “...proteins were identified in more than one spot. In one protein spot (#1464), two proteins (Rv1070c and Rv2716) were identified. Fig. 3. Representative two-dimensional DIGE image of culture filtrate proteins from log phase and starvation conditions. The total amount of protein used for CyDye labeling was...”
- ald of Mycobacterium tuberculosis encodes both the alanine dehydrogenase and the putative glycine dehydrogenase
Giffin, Journal of bacteriology 2012 - “...Rv1133c DnaK HspX Acg Ald Hrp1 MetE Rv1070c EchA8 Heat shock 70-kDa protein -Crystallin/URB-1 Unknown function L-Alanine dehydrogenase Hypoxic response protein...”
- The TB Structural Genomics Consortium: a decade of progress
Chim, Tuberculosis (Edinburgh, Scotland) 2011 - “.... We have determined the atomic level structure of the prokaryotic crotonase from Mtb , Rv1070c (manuscript in preparation). The overall fold of the prokaryotic crotonase is similar to the eukaryotic crotonase. The oligomerization state of the Mtb crotonase is that of hexamers and nonamers as...”
PFL_3064 enoyl-CoA hydratase/isomerase FadB1x from Pseudomonas fluorescens Pf-5
29% identity, 94% coverage
AT4G16210 ECHIA (ENOYL-COA HYDRATASE/ISOMERASE A); catalytic from Arabidopsis thaliana
Q6NL24 Probable enoyl-CoA hydratase 1, peroxisomal from Arabidopsis thaliana
33% identity, 74% coverage
- Dehydrin Client Proteins Identified Using Phage Display Affinity Selected Libraries Processed With Paired-End Phage Sequencing
Unêda-Trevisoli, Molecular & cellular proteomics : MCP 2024 - “...AT3G50240 2/3 1/3 75 2 2 1 KINESIN-RELATED PROTEIN, ATP-binding microtubule motor family protein, (KICP-02). AT4G16210 2/3 1/3 8 5 2 1 ENOYL-COA HYDRATASE/ISOMERASE A (ECHIA). ENOYL-COA HYDRATASE2 (E-COAH-2). AT4G19710 1/3 2/3 1 41 1 2 ASPARTATE KINASE-HOMOSERINE DEHYDROGENASE II (AK-HSDH II). plastidial location? AT4G20850 1/3...”
- “...that were bound by the DHNs in this study. Those were AT3G24080 [KRR1 family protein], AT4G16210 [ENOYL-COA HYDRATASE 2], and AT4G27170 [SESA 4] ( Supplemental Figs.S23 , S28 , and S32 , respectively). The other two proteins containing this motif were AT1G16800 [P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE...”
- Quantitative Proteomics Reveals the Dynamic Regulation of the Tomato Proteome in Response to Phytophthora infestans
Fan, International journal of molecular sciences 2021 - “...(ACAD) AT3G51840 3 0.057 0.872 3 0.050 0.928 3 2.085 0.014 Solyc01g059830 enoyl-CoA hydratase (ECH) AT4G16210 3 0.469 0.386 2 0.156 NQ 3 0.750 0.041 Solyc12g094450 enoyl-CoA hydratase (ECH) AT1G76150 2 0.278 NQ 3 0.202 0.216 3 1.663 0.002 a Number of biological replicates in which...”
- Multilevel Regulation of Peroxisomal Proteome by Post-Translational Modifications
Sandalio, International journal of molecular sciences 2019 - “...ps AT3G51840 acyl-CoA oxidase 4 na; nt; ph; ps AT4G04320 malonyl-CoA decarboxylase family protein ph AT4G16210 enoyl-CoA hydratase/isomerase A ac; mo; na; nt; ph; ps AT4G27780 acyl-CoA binding protein 2 ph AT4G29010 Enoyl-CoA hydratase/isomerase family ac; mo; na; nt; ps; ca AT5G16370 acyl activating enzyme 5...”
- Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses
Lin, Plant & cell physiology 2016 - “...LW10802 LW5678 NA AT2G39260 RNA binding LW1323 NA CAN66130 NA a Mpo-miR529c.1 LW4860 LW7434 NA AT4G16210 Enoyl-CoA hydratase/isomerase A LW8291 NA AT5G56110 R2R3 MYB transcription factor Mpo-miR529c.2 LW2562 NA AT2G40260 Homeodomain-like superfamily LW2626 NA AT1G76810 Translation initiation factor Mpo-miR529c.3 LW1838 NA AT1G66350 Negative regulator of gibberellin...”
- “...33 a NA, not available. Mpo-miR529c.1 targets LW1323 (112 degradome reads) and the ENOYL-COA HYDRATASE2 (AT4G16210) homolog-LW7434 (102 degradome reads). Mpo-miR529c.2 targets the MYB TF (AT5G56110) homolog-LW8291 (17 degradome reads) and the homeodomain TF (AT2G40260) homolog-LW2562 (13 degradome reads), whereas Mpo-miR529c.3 targets the translation initiation factor...”
- Defining the plant peroxisomal proteome: from Arabidopsis to rice
Kaur, Frontiers in plant science 2011 - “...2009 ), Goepfert et al. ( 2008 ), Zolman et al. ( 2008 ) ECHIA At4g16210 Monofunctional enoyl-CoA hydratase/isomerase a SKL LOC_Os03g19680 SKL Eubel et al. ( 2008 ), Reumann et al. ( 2007 , 2009 ) HIT1 At4g16566 Histidine triad family protein 1 SKV LOC_Os01g59750*...”
- The proteome map of spinach leaf peroxisomes indicates partial compartmentalization of phylloquinone (vitamin K1) biosynthesis in plant peroxisomes
Babujee, Journal of experimental botany 2010 (PubMed)- “...SRL>) and two enoyl-CoA hydratases/isomerases (ECHIa, At4g16210, SKL>; NS/ECHId, At1g60550, RLx5HL). The peroxisomal localization of the three proteins...”
- “...and two monofunctional enoyl-CoA hydratases/isomerases (ECHIa, At4g16210, 265 residues; SKL>, NS/ECHId, At1g60550, 337 residues, RLx5HL) were isolated from...”
- Peroxisome biogenesis and function
Kaur, The arabidopsis book 2009 - “...2007). Five additional proteins (BSMDR, At1g49670; ECHIa, At4g16210; ECHIc, At1g65520; SDRa, At4g05530; and NBP/HIT1, At4g16566) from this list have...”
- “...orthologs for these three proteins (AtSDRa, At4g05530; AtECHIa, At4g16210; AtHCDH, At3g15290) were also identified and proven to be peroxisomal targeted in vivo...”
- Phylogenomic study of lipid genes involved in microalgal biofuel production-candidate gene mining and metabolic pathway analyses
Misra, Evolutionary bioinformatics online 2012 - “...* , A4RUY4 Q01C53 * , CMC137C Enoyl-CoA hydratase (ECH) 4.2.1.17 KOG1680 KOG1679 K01692 GO:0004300 Q6NL24, O23468 , Q0WRQ2 , Q9T0K7 A8I9B0 , D8TRG5 * A4SBD9 Q010Z7 CMK139C CMT074C Enoyl-CoA reductase (TER) 1.3.1.38 KOG1639 K10258 GO:0019166 Q8LCU7 , F4J6R6 * , Q9M2U2 A8HM32 , A8JAQ9 D8THB1...”
- “...D8U2A4 * A4RQF1 Q01H50 * , CML080C Enoyl-CoA hydratase (ECH) 4.2.1.17 KOG1680 KOG1679 K01692 GO:0004300 Q6NL24, O23468 , Q0WRQ2 , Q9T0K7 A8I9B0 , D8TRG5 * A4SBD9 , A4S307 Q010Z7 CMK139C CMT074C 3-hydroxyacyl-CoA dehydrogenase (CHAD) 1.1.1.35 KOG2304 K00074 GO:0008691 GO:0003857 Q9LDF5 , Q9ZPI5, Q9ZPI6 A8IVP3 , D8UMK6...”
FGSG_13111 enoyl-CoA hydratase from Fusarium graminearum PH-1
27% identity, 82% coverage
NGR_c37150 enoyl-CoA hydratase from Sinorhizobium fredii NGR234
NGR_c37150 predicted enoyl-CoA hydratase/isomerase family protein from Rhizobium sp. NGR234
32% identity, 94% coverage
- Screening of metagenomic and genomic libraries reveals three classes of bacterial enzymes that overcome the toxicity of acrylate
Curson, PloS one 2014 - “...are adjacent to each other on the large plasmid pNGR234b, but are unlinked to vutD (NGR_c37150) and vutG (NGR_c03390), which are both located (but separately from each other) on the S. fredii chromosome ( Figure 4 ). The VutD and VutE Enzymes of S. fredii NGR234...”
- “...the valine oxidation pathway in S. fredii itself, we made insertional, genomic mutations in vutD (NGR_c37150) and vutE (NGR_b20860) of S. fredii , using pBIO1879, a Spc R -resistant derivative of the widely used suicide plasmid pK19 mob (see Materials and Methods). One ratified mutant for...”
T1E_5590 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG from Pseudomonas putida DOT-T1E
32% identity, 92% coverage
CD630_08000, CDR20291_0731 enoyl-CoA hydratase-related protein from Clostridioides difficile R20291
26% identity, 95% coverage
U3J3G1 Enoyl-CoA hydratase, mitochondrial from Anas platyrhynchos platyrhynchos
30% identity, 86% coverage
P34559 Probable enoyl-CoA hydratase, mitochondrial from Caenorhabditis elegans
28% identity, 87% coverage
AF0963 enoyl-CoA hydratase (fad-3) from Archaeoglobus fulgidus DSM 4304
28% identity, 95% coverage
CDR20291_0731 3-hydroxybutyryl-CoA dehydratase (crotonase) from Clostridium difficile R20291
26% identity, 93% coverage
WP_172595718 crotonase/enoyl-CoA hydratase family protein from Nocardia brasiliensis
36% identity, 68% coverage
MAP1017c EchA8_1 from Mycobacterium avium subsp. paratuberculosis str. k10
29% identity, 96% coverage
- Lymphoproliferative and gamma interferon responses to stress-regulated Mycobacterium avium subsp. paratuberculosis recombinant proteins
Gurung, Clinical and vaccine immunology : CVI 2014 - “...MAP0187c MAP2487c MAP3393c MAP3268 MAP1560 MAP1588c MAP1589c MAP1017c Superoxide dismutase Carbonic anhydrase IMP biosynthesis Heat shock proteins 18_3 and 18_2...”
- “...other four recombinant antigens (MAP2487c, MAP3393c, MAP3268, and MAP1017c). The IFN- response to the majority of the antigens was stronger in the vaccinated or...”
- Antigenicity of recombinant maltose binding protein-Mycobacterium avium subsp. paratuberculosis fusion proteins with and without factor Xa cleaving
Gurung, Clinical and vaccine immunology : CVI 2013 - “...MAP0184c, MAP1586, MAP3555, MAP3864 MAP0508, MAP0516c, MAP1017c, MAP2698c, MAP2872c, MAP3190, MAP3577, MAP3651c MAP0187c, MAP0540, MAP1560, MAP1885c, MAP2411,...”
- “...performed with MBP fusion proteins (MAP0435c, MAP1846c, and MAP1017c) to determine the optimal time for enzymatic cleavage of the M. avium subsp....”
- In silico identification of epitopes in Mycobacterium avium subsp. paratuberculosis proteins that were upregulated under stress conditions
Gurung, Clinical and vaccine immunology : CVI 2012 - “...of intermediate-affinity binding. Five proteins (MAP1589c, MAP4340, MAP1017c, MAP3974c, and MAP3268) did not reveal any epitopes with intermediate- or...”
- “...to stress conditions Mass (kDa) MAP0187c MAP0540 MAP1017c MAP1168c MAP1297 MAP1560 MAP1588c MAP1589c MAP1653 MAP1698c MAP1834c MAP2058c MAP2312c MAP2487c...”
1dubA / P14604 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 92% coverage
- Ligand: acetoacetyl-coenzyme a (1dubA)
PP3283 enoyl-CoA hydratase/isomerase PhaB from Pseudomonas putida KT2440
PP_3283 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG from Pseudomonas putida KT2440
32% identity, 92% coverage
ECHS1 / Q58DM8 short chain enoyl-CoA hydratase subunit (EC 4.2.1.150) from Bos taurus (see 5 papers)
NP_001020377 enoyl-CoA hydratase, mitochondrial precursor from Bos taurus
28% identity, 84% coverage
Q1D5U2 3-hydroxybutyryl-CoA dehydratase from Myxococcus xanthus (strain DK1622)
30% identity, 95% coverage
- Proteome Analyses of Soil Bacteria Grown in the Presence of Potato Suberin, a Recalcitrant Biopolymer
Sidibé, Microbes and environments 2016 - “...0.10 I Q1D340 malonyl CoA-acyl carrier protein transacylase 0.11 I Q1D5U1 3-hydroxyacyl-CoA dehydrogenase 0.18 I Q1D5U2 enoyl CoA dehydratase 0.13 I Q1D4E4 acyl-CoA dehydrogenase 0.07 I Q1D0T9 acetyl CoA carboxylase 0.03 I Q1D555 acetyl-coenzyme A carboxylase carboxyl transferase 0.06 I Q1D234 acetyl-CoA acetyltransferase 0.05 I Q1CZK4...”
- “...Q1D3D6, Q1D4E4 b , Q1D5Y1 b , Q1CZW5, A0A0H4WWQ8 enoyl-CoA hydratase I4WR77 b , I4WPL0 Q1D5U2 b 3-hydroxyacyl-CoA dehydrogenase I4WIC4, I4VRU7 a Q1D5U1 b , Q1D233 b acetyl-CoA acetyltransferase I4WBZ6, I4WIC3 Q1D5VO, Q1D234 b , BKT b , Q1D003 a Regulation of the fatty acid utilization...”
ECHM_RAT / P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 6 papers)
P14604 enoyl-CoA hydratase (EC 4.2.1.17); DELTA3,5-DELTA2,4-dienoyl-CoA isomerase (EC 5.3.3.21) from Rattus norvegicus (see 6 papers)
NP_511178 enoyl-CoA hydratase, mitochondrial precursor from Rattus norvegicus
29% identity, 84% coverage
- function: Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl- CoA) to the corresponding 3(S)-3-hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:10074351, PubMed:7883013). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:10074351, PubMed:7883013). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl- CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl- CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2- methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (By similarity). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:7883013). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl- CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA) (PubMed:10074351).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA (RHEA:45228)
catalytic activity: (3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA (RHEA:45736)
catalytic activity: (3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:26558)
catalytic activity: 3-hydroxyisovaleryl-CoA = 3-methyl-(2E)-butenoyl-CoA + H2O (RHEA:31079)
catalytic activity: 3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O (RHEA:26518)
catalytic activity: 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:45584)
catalytic activity: (2E)-2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl- CoA (RHEA:31175)
catalytic activity: (3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O (RHEA:30547)
catalytic activity: (3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O (RHEA:31191)
subunit: Homohexamer; dimer of trimers. - Acetylation-Mediated Post-Translational Modification of Pyruvate Dehydrogenase Plays a Critical Role in the Regulation of the Cellular Acetylome During Metabolic Stress
Rajakumar, Metabolites 2024 - “...c oxidase subunit 4 isoform 1, mitochondrial 29 P10888 UP 78 Enoyl-CoA hydratase, mitochondrial 101 P14604 UP 79 Succinate dehydrogenase [ubiquinone] ironsulfur subunit, mitochondrial 64 P21913 NC 80 10 kDa heat shock protein, mitochondrial 80 P26772 UP 81 Peptidyl-prolyl cistrans isomerase F, mitochondrial 72 P29117 UP...”
- The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity.
Ji, PloS one 2022 - “...P12007 Ivd Isovaleryl-CoA dehydrogenase, mitochondrial 1.73 0.008189 THTR_RAT P24329 Tst Thiosulfate sulfurtransferase 1.73 0.009805 ECHM_RAT P14604 Echs1 Enoyl-CoA hydratase, mitochondrial 1.72 0.001788 ACON_RAT Q9ER34 Aco2 Aconitate hydratase, mitochondrial 1.71 0.002251 CALR_RAT P18418 Calr Calreticulin 1.71 0.00505 PPIF_RAT P29117 Ppif Peptidyl-prolyl cis-trans isomerase F, mitochondrial 1.70 0.002718...”
- Space Radiation-Induced Alterations in the Hippocampal Ubiquitin-Proteome System
Tidmore, International journal of molecular sciences 2021 - “...Exposure Marker (TEM) signature proteins. UniProt Accession Numbers F1LQ48 * P13803 Q07303 Q4KLM4 * O35763 P14604 Q02874 Q5M9I5 O70511 P18484 P62243 * Q63803 P09330 P21396 P53676 Q68FS2 * P13638 P26772 P48679 * Q91XU8 Fully mapped and annotated in UniProt. * Denotes matched to UPS data base....”
- Myocardial proteomic profile in pulmonary arterial hypertension.
Hołda, Scientific reports 2020 - “...1.19 P14408 Fh Fumarate hydratase, mitochondrial 1.09 P11951 Cox6c2 Cytochrome c oxidase subunit 6C-2 1.19 P14604 Echs1 Enoyl-CoA hydratase, mitochondrial 1.10 P07895 Sod2 Superoxide dismutase [Mn], mitochondrial 1.19 Q9ER34 Aco2 Aconitate hydratase, mitochondrial 1.10 P07340 Atp1b1 Sodium/potassium-transporting ATPase subunit beta-1 1.20 P12007 Ivd Isovaleryl-CoA dehydrogenase, mit...”
- Transcriptome analysis of genes and pathways associated with metabolism in Scylla paramamosain under different light intensities during indoor overwintering.
Li, BMC genomics 2020 - “...dipeptidase Q96KP4 0.1312 0.0006 0.2145 0.0087 Spermidine synthase Q64674 0.5022 0.0310 0.5562 0.0409 Enoyl-CoA hydratase P14604 0.4812 0.0056 Alpha-aminoadipic semialdehyde dehydrogenase Q2KJC9 0.4374 0.0041 Fatty acid elongation (ko00062) Lysosomal thioesterase PPT2 O70489 3.4756 0.0236 3.1206 0.0462 Lysosomal thioesterase PPT2-B Q6GNY7 1.9706 0.0252 3.0013 1.27E-05 3-ketoacyl-CoA thiolase...”
- Inflammation and apoptosis accelerate progression to irreversible atrophy in denervated intrinsic muscles of the hand compared with biceps: proteomic analysis of a rat model of obstetric brachial plexus palsy.
Yu, Neural regeneration research 2020 - “...P12785 * , A0A0G2K5G8 * , Q63151 * , P33124, P17764, Q9WVK7, Q9WVK3, P08503, P70584, P14604, Q5M9H2, Q63704 0.002 Pathways at 5 weeks Glycolysis/gluconeogenesis 29 G3V9W6 * , P25113 * , D3ZZN3, P07323, P30835, A0A0G2JZH8, Q6P9U7, E9PTN6, D4A5G8, Q9Z1N1, B1WBN9, P49432, E9PTV9, Q6P6R2, P08461, P27881, P09117,...”
- “...0.001 Fatty acid metabolism 18 O35547 * , Q63151 * , P70584, Q9WVK3, P33124, G3V9U2, P14604, P17764, P15651, Q64428, Q5M9H2, Q9WVK7, Q60587, P18163, P18886, P08503, P07896, G3V7N5 < 0.001 Calcium signaling 16 P11275, P20651, A0A0G2K9C8, P13286, Q64578, A0A0G2JSR0, F1LLZ7, Q304F3, G3V731, A0A0G2K5J1, F1LQL1, P29117, Q9Z2L0, Q05962,...”
- 2-Methoxyestradiol protects against pressure overload-induced left ventricular hypertrophy
Maayah, Scientific reports 2018 - “...Enoyl-CoA delta isomerase 1 0.48 (0.025) 0.97 (0.921) P45953 Acyl-CoA dehydrogenase 0.59 (0.025) 1.44 (0.15) P14604 Enoyl-CoA hydratase 0.60 (0.039) 1.01 (0.97) Q64591 2,4-Dienoyl-CoA reductase 0.39 (0.145) 2.76 (0.038) Q5XIT9 Methylcrotonoyl-CoA carboxylase beta chain 0.85 (0.086) 1.63 (0.041) P35738 2-Oxoisovalerate dehydrogenase 0.63 (0.028) 2.61 (0.14) Q920L2...”
- Quantitative proteomic analysis of intracerebral hemorrhage in rats with a focus on brain energy metabolism.
Liu, Brain and behavior 2018 - “...Inpp5j Phosphatidylinositol 4,5bisphosphate 5phosphatase A 0.72 P13221 44.91 71.43 54 Got1 Aspartate aminotransferase, cytoplasmic 0.74 P14604 24.5 53.45 20 Echs1 EnoylCoA hydratase, mitochondrial 0.76 Inflammation and stress Q63041 68.56 29.87 53 Pzp Alpha1macroglobulin 25.40 M0RBF1 71.38 31.21 49 C3 Complement C3 23.34 P24090 12.52 35.80 10...”
- More
- Functional proteomic analysis of nonalcoholic fatty liver disease in rat models: enoyl-coenzyme a hydratase down-regulation exacerbates hepatic steatosis.
Zhang, Hepatology (Baltimore, Md.) 2010 (PubMed)- GeneRIF: enoyl-coenzyme a hydratase down-regulation exacerbates hepatic steatosis.
H16_A0865 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
32% identity, 96% coverage
Q7JR58 enoyl-CoA hydratase from Drosophila melanogaster
26% identity, 82% coverage
NP_823962, SAV_2786 enoyl-CoA hydratase from Streptomyces avermitilis MA-4680
33% identity, 89% coverage
- Distribution analysis of hydrogenases in surface waters of marine and freshwater environments
Barz, PloS one 2010 - “...YP_525330; Rmetalli, Ralstonia metallidurans CH34 YP_583693; Saverm, Streptomyces avermitilis MA-4680 NP_828541; Savermi Streptomyces avermitilis MA-4680 NP_823962; Scoelic Streptomyces coelicolor A3(2) NP_629596; Sdegra, Saccharophagus degradans 2-40 YP_526001; Smalto Stenotrophomonas maltophilia R551-3 YP_002027502; Ssedimi, Shewanella sediminis HAW-EB3 YP_001475080; Sulfuro, Sulfurovum sp. NBC37-1 YP_001358952; Xcamp Xanthomonas campestris pv. vesicatoria...”
- Biochemical analysis of the biosynthetic pathway of an anticancer tetracycline SF2575
Pickens, Journal of the American Chemical Society 2009 - “...42% / 57% Q7D785 SsfI PlmI DAHP-7-phosphate synthase 45 kDa 56% / 67% AY354515 SsfJ SAV_2786 Enoyl CoA hydratase/isomerase 28 kDa 61% / 75% BAC70497 SsfK Athe_1156 3-Ketoacyl-(ACP) reductase 26 kDa 38% / 59% ACM60257 SsfL1 SdgA Salicylyl-AMP ligase 58kDa 75% / 84% BAC78380 SsfL2 OxyH...”
CPF_0090 3-hydroxybutyryl-CoA dehydratase from Clostridium perfringens ATCC 13124
28% identity, 91% coverage
PHATRDRAFT_55192 hydratase enyol-coa hydratase from Phaeodactylum tricornutum CCAP 1055/1
29% identity, 91% coverage
- Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum
Feng, Scientific reports 2015 - “...but also supplied P for cell growth. Also, we observed the downregulation of enoyl-CoA hydratase (PHATRDRAFT_55192), which is responsible for hydrating the double bond between the second and third carbons on acyl-CoA, also known as crotonase important in catalyzing fatty acids to produce acetyl-CoA and energy...”
- “...(PHATRDRAFT_20342) and adenylosuccinate synthase (PHATRDRAFT_26256) involved in alanine, aspartate and glutamate metabolism, and enoyl-CoA hydratase (PHATRDRAFT_55192) involved in tryptophan metabolism. Among these, downregulation of glutamate synthase would cause a reduction of glutamate which plays an important role in transamination of amino acids in amino acid metabolism...”
ANACAC_03496 hypothetical protein from Anaerostipes caccae DSM 14662
29% identity, 93% coverage
ECHS1 / P30084 short-chain enoyl-CoA hydratase monomer (EC 4.2.1.17) from Homo sapiens (see 7 papers)
ECHM_HUMAN / P30084 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Homo sapiens (Human) (see 6 papers)
P30084 enoyl-CoA hydratase (EC 4.2.1.17) from Homo sapiens (see 3 papers)
NP_004083 enoyl-CoA hydratase, mitochondrial from Homo sapiens
29% identity, 87% coverage
- function: Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl- CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:25125611, PubMed:26251176). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:26251176). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3- methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA) (PubMed:26251176). Can bind tiglyl-CoA (2- methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (PubMed:26251176). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:25125611, PubMed:26251176). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)- hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl- CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA (RHEA:45228)
catalytic activity: (3E)-hexenoyl-CoA = (2E)-hexenoyl-CoA (RHEA:45736)
catalytic activity: (3S)-3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:26558)
catalytic activity: 3-hydroxyisovaleryl-CoA = 3-methyl-(2E)-butenoyl-CoA + H2O (RHEA:31079)
catalytic activity: 3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O (RHEA:26518)
catalytic activity: 3-hydroxybutanoyl-CoA = (2E)-butenoyl-CoA + H2O (RHEA:45584)
catalytic activity: (2E)-2-methylpropenoyl-CoA + H2O = (S)-3-hydroxyisobutanoyl- CoA (RHEA:31175)
catalytic activity: (3S)-hydroxyhexanoyl-CoA = (2E)-hexenoyl-CoA + H2O (RHEA:30547)
catalytic activity: (3S)-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O (RHEA:31191)
subunit: Homohexamer; dimer of trimers. - Molecular and in silico investigation of a novel ECHS1 gene mutation in a consanguine family with short-chain enoyl-CoA hydratase deficiency and Mt-DNA depletion: effect on trimer assembly and catalytic activity.
Maalej, Metabolic brain disease 2024 (PubMed)- GeneRIF: Molecular and in silico investigation of a novel ECHS1 gene mutation in a consanguine family with short-chain enoyl-CoA hydratase deficiency and Mt-DNA depletion: effect on trimer assembly and catalytic activity.
- Delineating the neurological phenotype in children with defects in the ECHS1 or HIBCH gene.
Marti-Sanchez, Journal of inherited metabolic disease 2021 (PubMed)- GeneRIF: Delineating the neurological phenotype in children with defects in the ECHS1 or HIBCH gene.
- ECHS1 disease in two unrelated families of Samoan descent: Common variant - rare disorder.
Simon, American journal of medical genetics. Part A 2021 - GeneRIF: ECHS1 disease in two unrelated families of Samoan descent: Common variant - rare disorder.
- ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation.
Li, Cell death & disease 2021 - GeneRIF: ECHS1, an interacting protein of LASP1, induces sphingolipid-metabolism imbalance to promote colorectal cancer progression by regulating ceramide glycosylation.
- Inactivation of the AMPK-GATA3-ECHS1 Pathway Induces Fatty Acid Synthesis That Promotes Clear Cell Renal Cell Carcinoma Growth.
Qu, Cancer research 2020 (PubMed)- GeneRIF: In clear cell renal cell carcinoma (ccRCC) ECHS1 downregulation induced fatty acid (FA) and branched-chain amino acid (BCAA) accumulation, which inhibited AMPK-promoted expression of GATA3, a transcriptional activator of ECHS1. BCAA accumulation induced activation of mTORC1 and de novo FA synthesis and promoted cell proliferation. GATA3 expression phenocopied ECHS1 in predicting ccRCC progression and patient survival.
- ECHS1 suppresses renal cell carcinoma development through inhibiting mTOR signaling activation.
Wang, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 (PubMed)- GeneRIF: ECHS1 suppresses renal cell carcinoma development through inhibiting mTOR signaling activation.
- Novel ECHS1 mutations in Leigh syndrome identified by whole-exome sequencing in five Chinese families: case report.
Sun, BMC medical genetics 2020 - GeneRIF: Novel ECHS1 mutations in Leigh syndrome identified by whole-exome sequencing in five Chinese families: case report.
- Paroxysmal and non-paroxysmal dystonia in 3 patients with biallelic ECHS1 variants: Expanding the neurological spectrum and therapeutic approaches.
Illsinger, European journal of medical genetics 2020 (PubMed)- GeneRIF: Paroxysmal and non-paroxysmal dystonia in 3 patients with biallelic ECHS1 variants: Expanding the neurological spectrum and therapeutic approaches.
- More
- ProtGraph: a tool for the quick and comprehensive exploration and exploitation of the peptide search space derived from protein sequence databases using graphs.
Lux, Briefings in bioinformatics 2024 - “...233 HBB (P68871) VAR_002901 VAR_002904 541 HBB (P68871) VAR_002933 VAR_002934 VAR_002939 VAR_002941 2 175 ECHS1 (P30084) VAR_022274 427 HBA (P69905) VAR_002788 VAR_002795 VAR_002799 3 168 HBA (P69905) VAR_002788 VAR_002792 364 HBA (P69905) VAR_002788 VAR_002792 4 161 HBB (P68871) VAR_003077 335 HBB (P68871) VAR_002935 VAR_002937 VAR_002939 5...”
- Cortical lipid metabolic pathway alteration of early Alzheimer's disease and candidate drugs screen.
Wang, European journal of medical research 2024 - “...Protein symbol Full name Score Up-regulated network 1 P80404 ABAT 4-Aminobutyrate aminotransferase, mitochondrial 0.997 2 P30084 ECHS1 Enoyl-CoA hydratase, mitochondrial 0.992 3 Q9BQ95 ECSIT Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial 0.994 4 O14842 FFAR1 Free fatty acid receptor 1 0.993 5 P03923 MT-ND6 NADH-ubiquinone...”
- Proteome architecture of human-induced pluripotent stem cell-derived three-dimensional organoids as a tool for early diagnosis of neuronal disorders
Negi, Indian journal of pharmacology 2023 - “...12 1.04 6.44E-03 18 P52943 Cysteine-rich protein 2 CRIP2 4 22.5 36 1.08 2.18E-03 19 P30084 Enoyl-CoA hydratase, mitochondrial ECHS1 4 31.4 23 1.1 1.68E-03 20 Q14165 Malectin MLEC 2 32.2 10 3.21 1.14E-10 21 Q10471 Polypeptide N-acetylgalactosaminyltransferase GALNT2 2 64.7 5 1.19 2.22E-03 22 A0A024RC16...”
- “...9 55.4 1.27 9 Q10471 Polypeptide N-acetylgalactosaminyl transferase 2 GALNT2 5 2 64.7 1.19 10 P30084 Enoyl-CoA hydratase, mitochondrial ECHS1 23 4 31.4 1.1 11 P52943 Cysteine-rich protein 2 CRIP2 36 4 22.5 1.08 12 P17677 Neuromodulin GAP43 63 13 24.8 1.03 13 P14550 Alcohol dehydrogenase...”
- Shared and Unique Disease Pathways in Amyotrophic Lateral Sclerosis and Parkinson's Disease Unveiled in Peripheral Blood Mononuclear Cells.
Lualdi, ACS chemical neuroscience 2023 - “...5.6 0.164 597 PSME1 Q06323 proteasome activator complex subunit 1 28.7 5.8 0.207 618 ECHS1 P30084 enoyl-CoA hydratase, mitochondrial 31.4 8.3 0.183 635 YWHAZ P63104 1433proteinzeta/delta 27.7 4.7 0.151 639 ARPC2 O15144 actin-related protein 2/3 complex subunit 2 34.3 6.8 0.196 683 WDR1 O75083 WD repeat-containing...”
- CLPX regulates mitochondrial fatty acid β-oxidation in liver cells.
Suzuki, The Journal of biological chemistry 2023 - “...CS Citrate synthase, mitochondrial 3 14 P63167 DYNLL1 Dynein light chain 1, cytoplasmic 1 3 P30084 ECHS1 Enoyl-CoA hydratase, mitochondrial 7 29 P13804 ETFA Electron transfer flavoprotein subunit alpha, mitochondrial 5 8 P40939 HADHA Trifunctional enzyme subunit alpha, mitochondrial 10 17 P55084 HADHB Trifunctional enzyme subunit...”
- 2D-DIGE-Based Proteomic Profiling with Validations Identifies Vimentin as a Secretory Biomarker Useful for Early Detection and Poor Prognosis in Oral Cancers
Sivagnanam, Journal of oncology 2022 - “...1, SV = 3 [PRDX6_HUMAN] 49.05 51.79 3 9 13 31 224 25.0 6.38 4 P30084 Enoyl CoAhydratase, mitochondrialOS = Homosapiens, GN = ECHS1, PE = 1, SV = 4 [ECHM_HUMAN] 63.59 45.86 1 9 15 39 290 31.4 8.07 5 P52565 RhoGDP dissociationinhibitor1OS = HomosapiensGN...”
- Dissecting Regulators of Aging and Age-Related Macular Degeneration in the Retinal Pigment Epithelium.
Karunadharma, Oxidative medicine and cellular longevity 2022 - “...59.1/6.3 56.8/5.98 25 11 20 D22 0.0078 2,3 0.0037, 0.0948 I, O Enoyl-CoA hydratase, mitochondrial P30084 ECHS1 29.4/6.6 28.3/5.88 48 14 30 Phosphoglycerate mutase 1 P18669 PGAM1 28.7/6.75 17 3 3 D23 0.0305 3,4 0.0266, 0.0887 I, A Aminoacylase 1 Q03154 ACY1 44.5/6.4 45.9/5.77 13 4...”
- HSP60 Regulates Lipid Metabolism in Human Ovarian Cancer
Li, Oxidative medicine and cellular longevity 2021 - “...sapiens GN=EHHADH PE=1 SV=3[ECHP_HUMAN] EHHADH 33.47 22 22 34 79.44 9.14 1.62 2.25 E 03 P30084 Enoyl-CoA hydratase, mitochondrial OS=Homo sapiens GN=ECHS1 PE=1 SV=4[ECHM_HUMAN] ECHS1 75.86 19 19 156 31.37 8.07 1.52 3.56 E 03 Q08426 Peroxisomal bifunctional enzyme OS=Homo sapiens GN=EHHADH PE=1 SV=3[ECHP_HUMAN] EHHADH 33.47...”
- “...sapiens GN=EHHADH PE=1 SV=3[ECHP_HUMAN] EHHADH 33.47 22 22 34 79.44 9.14 1.62 2.25 E 03 P30084 Enoyl-CoA hydratase, mitochondrial OS=Homo sapiens GN=ECHS1 PE=1 SV=4[ECHM_HUMAN] ECHM 75.86 19 19 156 31.37 8.07 1.52 3.56 E 03 Q16836 Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial OS=Homo sapiens GN=HADH PE=1 SV=3[HCDH_HUMAN] HCDH...”
- More
SS1G_00237 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
29% identity, 86% coverage
BP0627 probable enoyl-CoA hydratase/isomerase from Bordetella pertussis Tohama I
29% identity, 96% coverage
- Avirulent phenotype promotes Bordetella pertussis adaptation to the intramacrophage environment
Farman, Emerging microbes & infections 2023 - “...amino acids, required for the metabolism of fatty acids (acetyl-CoA synthetase BP0661 , enoyl-CoA hydratases BP0627 and BP0662 , and long-chain fatty acid ligase fadD ), and for phenylacetic acid catabolic pathway ( BP2675BP2684 ), was significantly increased. Large part of the BvgAS regulon is modulated...”
- Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution
Dienstbier, Emerging infectious diseases 2021 - “...Group_2206 BP0624 2.2 2.5 Substrate-CoA ligase toh_00607 Group_2604 BP0625 2.3 3.3 Acyl-CoA dehydrogenase toh_00609 Group_2725 BP0627 2.0 3.3 Enoyl-CoA hydratase/isomerase toh_00610 Group_895 BP0628 2.4 2.8 Pyruvate dehydrogenase component toh_00611 pdhA BP0629 2.3 2.8 Pyruvate dehydrogenase component toh_01576 Group_23 WP_003811211.1 6.8 3.3 Capsular biosynthesis protein toh_01584 wza...”
- The multifaceted RisA regulon of Bordetella pertussis
Coutte, Scientific reports 2016 - “...to non-modulated BPSM ( Fig. 3 ). Only 6 genes fall in this cluster ( bp0627, bp0628, bp1704, bp2496, bp3501, bp3871 ), all of unknown function. Based on their expression in modulated BPSM, these genes would be classified as vrg s. However, their expression does not...”
2hw5C / P30084 The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
29% identity, 96% coverage
- Ligand: crotonyl coenzyme a (2hw5C)
LOC21392597 probable enoyl-CoA hydratase 1, peroxisomal from Morus notabilis
34% identity, 70% coverage
- Genome-Wide Identification of Candidate Genes Associated with Heat Stress in Mulberry (Morus alba L.)
Jin, Current issues in molecular biology 2023 - “...aminotransferase 1 (LOC21390509), isovaleryl-CoA dehydrogenase (LOC21397545), methylcrotonoyl-CoA carboxylase subunit alpha (LOC21385626), probable enoyl-CoA hydratase 1 (LOC21392597), aldehyde dehydrogenase family 3 (LOC21396538) and alanine--glyoxylate aminotransferase (LOC21404820). In plants, the catabolism of amino acids is extremely important in metabolic stress (e.g., when there are limited carbohydrates during prolonged...”
BPHYT_RS17335 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
30% identity, 95% coverage
- mutant phenotype: Specifically important for utilization of phenylacetate and phenylalanine. 51% identical to the characterized enzyme (paaF = Q845K2) from Pseudomonas sp. Y2, which is part of the aerobic phenylacetyl-CoA pathway. Also, 83% identical to enoyl-CoA hydratase Crt2 = H16_A3307 from Ralstonia eutropha (PMID:30243533).
LK421_13465 enoyl-CoA hydratase-related protein from Coprococcus eutactus ATCC 27759
30% identity, 91% coverage
CNAG_04531 enoyl-CoA hydratase from Cryptococcus neoformans var. grubii H99
27% identity, 90% coverage
- Cryptococcus neoformans resists to drastic conditions by switching to viable but non-culturable cell phenotype
Hommel, PLoS pathogens 2019 - “...Genes Corrected p value (FDR 1%) Fatty acid degradation CNAG_00490, CNAG_00524, CNAG_03019, CNAG_02489, CNAG_06628, CNAG_07747, CNAG_04531, CNAG_04688 1.20E-09 Valine, leucine and isoleucine degradation CNAG_00484, CNAG_00490, CNAG_00524, CNAG_03067, CNAG_06628, CNAG_04531, CNAG_04351, CNAG_04688 2.40E-08 Fatty acid metabolism CNAG_00490, CNAG_00524, CNAG_03019, CNAG_07747, CNAG_04531, CNAG_04688 9.50E-07 Propanoate metabolism CNAG_00797, CNAG_06628,...”
- “...CNAG_04351, CNAG_04217, CNAG_04688 4.00E-05 Biosynthesis of secondary metabolites CNAG_00484, CNAG_00490, CNAG_00524, CNAG_00797, CNAG_03067, CNAG_02489, CNAG_06628, CNAG_04531, CNAG_04217, CNAG_04688, CNAG_06431 2.60E-04 Peroxisome CNAG_00537, CNAG_03067, CNAG_03019, CNAG_06551, CNAG_07747 2.90E-04 Metabolic pathways CNAG_00484, CNAG_00490, CNAG_00524, CNAG_00797, CNAG_00826, CNAG_03067, CNAG_03019, CNAG_01540, CNAG_02489, CNAG_06628, CNAG_07747, CNAG_05653, CNAG_05303, CNAG_04531, CNAG_04351, CNAG_04217, CNAG_04688...”
Saci_2208 3-hydroxybutyryl-CoA dehydrogenase from Sulfolobus acidocaldarius DSM 639
30% identity, 40% coverage
- Impact of nutrient excess on physiology and metabolism of <i>Sulfolobus acidocaldarius</i>
Sedlmayr, Frontiers in microbiology 2024 - “...saci_1054 Acyl-CoA synthetase 3.0 1.6 saci_1134 3-Hydroxyacyl-CoA dehydrogenase 2.6 1.2 saci_2148 Acyl-CoA synthetase 2.1 1.1 saci_2208 3-Hydroxyacyl-CoA dehydrogenase 2.8 1.4 saci_2209 Acetyl-CoA acetyltransferase 4.0 2.1 saci_2211 Acyl-CoA synthetase 2.4 2.1 saci_2219 Sterol carrier protein 2.0 1.5 saci_2232 Acetyl-CoA acetyltransferase 2.8 1.8 saci_2233 Acetyl-CoA acetyltransferase 3.0 1.9...”
acuK / C8YX87 acryloyl-CoA hydratase/3-hydroxypropanoyl-CoA hydrolase (EC 3.1.2.4; EC 4.2.1.116) from Halomonas sp. HTNK1 (see paper)
30% identity, 96% coverage
H281DRAFT_05725 2,3-dehydroadipyl-CoA hydratase / enoyl-CoA hydratase (EC 4.2.1.17) from Paraburkholderia bryophila 376MFSha3.1
30% identity, 95% coverage
- mutant phenotype: Specifically important for phenylacetate utilization. 51% identical to the characterized enzyme (paaF = Q845K2) from Pseudomonas sp. Y2, which is part of the aerobic phenylacetyl-CoA pathway. Also, 84% identical to enoyl-CoA hydratase Crt2 = H16_A3307 from Ralstonia eutropha (PMID:30243533).
CNBH0240 hypothetical protein from Cryptococcus neoformans var. neoformans B-3501A
27% identity, 90% coverage
A1WF10 Short chain enoyl-CoA hydratase from Verminephrobacter eiseniae (strain EF01-2)
32% identity, 95% coverage
HCAG_02218 hypothetical protein from Histoplasma mississippiense (nom. inval.)
29% identity, 78% coverage
ACTT3_ALTAL / Q589W8 Enoyl-CoA hydratase ACTT3; ACT-toxin biosynthesis protein 3; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata) (see 5 papers)
29% identity, 82% coverage
- function: Enoyl-CoA hydratase; part of the gene clusters that mediate the biosynthesis of the host-selective toxins (HSTs) ACT-toxins responsible for brown spot of tangerine disease by the tangerine pathotype which affects tangerines and mandarins (PubMed:18944496, PubMed:18986255). ACT-toxins consist of three moieties, 9,10-epoxy-8- hydroxy-9-methyl-decatrienoic acid (EDA), valine and a polyketide (PubMed:22846083). ACT-toxin I is toxic to both citrus and pear; toxin II the 5''-deoxy derivative of ACT-toxin I, is highly toxic to pear and slightly toxic to citrus (PubMed:22846083). On cellular level, ACT- toxins affect plasma membrane of susceptible cells and cause a sudden increase in loss of K(+) after a few minutes of toxin treatment (PubMed:22846083). The acyl-CoA ligase ACTT1, the hydrolase ACTT2, the enoyl-CoA hydratases ACTT3 and ACTT6, and the acyl-CoA synthetase ACTT5 are all involved in the biosynthesis of the AK-, AF- and ACT-toxin common 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (EDA) structural moiety (PubMed:18944496, PubMed:18986255, PubMed:19271978). The exact role of each enzyme, and of additional enzymes identified within the AF-toxin clusters have still to be determined (PubMed:18944496, PubMed:18986255, PubMed:19271978). On the other hand, ACTTS1 to ACTTS4 are specific to the tangerine pathotype (PubMed:22846083). The function of ACTTS3 is to elongate the polyketide chain portion of ACT-toxin that is unique to this toxin (PubMed:20192828). The enoyl-reductase ACTTS2 might complement the missing enoyl-reductase (ER) domain in ACTTS3 in the synthesis of the polyketide portion of ACT-toxin (PubMed:20055645). The roles of the nonribosomal peptide synthetases-related proteins ACTTS1 and ACTTS4 have also still not been elucidated (PubMed:22846083).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (RHEA:20724)
BC4524 3-hydroxybutyryl-CoA dehydratase from Bacillus cereus ATCC 14579
27% identity, 89% coverage
MAVA5_16150 enoyl-CoA hydratase from Mycobacterium avium subsp. hominissuis A5
35% identity, 79% coverage
CNI00240 enoyl-CoA hydratase from Cryptococcus neoformans var. neoformans JEC21
27% identity, 90% coverage
WP_185876117 enoyl-CoA hydratase-related protein from Cetobacterium sp. 8H
27% identity, 92% coverage
BPSL3043 probable enoyl-CoA hydratase PaaG from Burkholderia pseudomallei K96243
29% identity, 97% coverage
BCAL0409 putative phenylacetic acid degradation enoyl-CoA hydratase PaaF from Burkholderia cenocepacia J2315
30% identity, 95% coverage
FRAAL4765 Putative enoyl-CoA hydratase from Frankia alni ACN14a
33% identity, 31% coverage
- Genomic Insights of Alnus-Infective Frankia Strains Reveal Unique Genetic Features and New Evidence on Their Host-Restricted Lifestyle
Kim, Genes 2023 - “...protein FRAAL1256 TM I Lipid transport and metabolism FRAAL2505 atoD Acetoacetyl-CoA transferase FRAAL2504, FRAAL3148, FRAAL3149 FRAAL4765 Putative enoyl-CoA hydratase FRAAL2509, FRAAL2514, FRAAL3092, FRAAL3517, FRAAL3973, FRAAL5910, FRAAL6774 FRAAL1660 Putative Acyl-CoA dehydrogenase FRAAL6459 J Translation, ribosomal structure and biogenesis FRAAL4260 Putative glutamyl-tRNA(Gln) amidotransferase, subunit A FRAAL0363, FRAAL3665, FRAAL6013,...”
Gmet_1716 Enoyl-CoA hydratase/isomerase from Geobacter metallireducens GS-15
31% identity, 91% coverage
- Genome sequence of a dissimilatory Fe(III)-reducing bacterium Geobacter soli type strain GSS01(T)
Yang, Standards in genomic sciences 2015 - “...acyl-CoA thioesterases (SE37_09325, SE37_09950, SE37_10860, SE37_14445 and SE37_15385), enoyl-CoA hydratases (SE37_15375; SE37_11185, 81% similarity to Gmet_1716 in G. metallireducens ), phenylacetate-CoA ligase (SE37_04405, SE37_06045 and SE37_06085) and acyl-CoA synthetase (SE37_06810). The ability to utilize aromatic compounds and other carbon sources may be due to stepwise breakdown...”
AKT31_ALTAL / Q9P4U9 Enoyl-CoA hydratase AKT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata) (see 4 papers)
29% identity, 82% coverage
- function: Enoyl-CoA hydratase; part of the gene clusters that mediate the biosynthesis of the host-selective toxins (HSTs) AK-toxins responsible for Japanese pear black spot disease by the Japanese pear pathotype (PubMed:10432635, PubMed:10975654, PubMed:20348386). AK- toxins are esters of 9,10-epoxy 8-hydroxy 9-methyldecatrienoic acid (EDA) (PubMed:22846083). On cellular level, AK-toxins affect plasma membrane of susceptible cells and cause a sudden increase in loss of K(+) after a few minutes of toxin treatment (PubMed:22846083). The acyl-CoA ligase AKT1, the hydrolase AKT2 and enoyl-CoA hydratase AKT3 are all involved in the biosynthesis of the AK-, AF- and ACT-toxin common 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (EDA) structural moiety (PubMed:10432635, PubMed:10975654, PubMed:22846083). Part of the EDA biosynthesis occurs in the peroxisome since these 3 enzymes are localized in peroxisomes (PubMed:20348386). The exact roles of the 3 enzymes, as well as of additional AK-toxin clusters enzymes, including AKT4, AKT6 and AKTS1, have still to be elucidated (PubMed:10432635, PubMed:10975654, PubMed:22846083). The Cytochrome P450 monooxygenase AKT7 on the other side functions to limit production of EDA and AK- toxin, probably via the catalysis of a side reaction of EDA or its precursor (PubMed:24611558).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (RHEA:20724)
disruption phenotype: Abolishes the production of AF-toxins and their precuror 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid; and impairs the pathogenicity.
BP3277 putative enoyl-CoA hydratase from Bordetella pertussis Tohama I
31% identity, 93% coverage
- Immuno-proteomics analysis between OMV of vaccine and dominant wild type strains of Bordetella pertussis in Iran
Badamchi, Iranian journal of microbiology 2020 - “...protein (spot 27; BP1616), Inorganic pyrophosphatase (spot 30; BP2533), a Putative enoyl-CoA hydratase (spot 25; BP3277). Five of protein spots were common among OMV of the vaccine strain tested and OMV of Bp IP91 including (BP2759), (BP2377), (BP2818), (BP3266) (BP0965), (BP0205) and BP0558. ( Figs. 4A...”
- “...subunit 64.8 6.1 596 cytoplasm 34 lpdA 5.872 Dihydrolipoyl dehydrogenase 62.3 6.1 596 cytoplasm 35 BP3277 45.45 Putative enoyl-CoA hydratase 30.1 6.2 275 cytoplasm 36 mmsB 19.19 3-hydroxyisobutyrate dehydrogenase 29.6 5.7 297 cytoplasm 37 BP2434 2.828 Periplasmic serine endoprotease DegPlike 52.1 7.6 495 periplasm, Outer membrane...”
BB4614 putative enoyl-CoA hydratase from Bordetella bronchiseptica RB50
31% identity, 96% coverage
D5RAC1 Enoyl-CoA hydratase/isomerase family protein from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 23726 / VPI 4351)
FN1020 3-hydroxybutyryl-CoA dehydratase from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
26% identity, 93% coverage
- Quantitative Proteomic Analysis of Outer Membrane Vesicles from Fusobacterium nucleatum Cultivated in the Mimic Cancer Environment
Zhang, Microbiology spectrum 2023 - “...D5RDA3 and D5RD18), formate C-acetyltransferase (D5RDZ7), acetyl-CoA C-acetyltransferase (D5RE94), 3-hydroxyacyl-CoA dehydrogenase (D5RAC0), and enoyl-CoA hydratase (D5RAC1), were downregulated, among which pyruvate synthase (D5RD18) was significantly downregulated by 0.42-fold. The downregulation of pyruvate synthase can inhibit the oxidative decarboxylation of pyruvate to form acetyl-CoA, reducing the amount...”
- The Pathogenicity of Fusobacterium nucleatum Modulated by Dietary Fibers-A Possible Missing Link between the Dietary Composition and the Risk of Colorectal Cancer
Nawab, Microorganisms 2023 - “...FN1170 (Por)) to Acetyl-CoA (FN0495 (Thl/atoB)), Acetoacetyl-CoA (Hbd (FN1019), (R) and (S)-3-Hydroxybutanoyl-CoA (CroR (FN0816), cro (FN1020)), and Crotonyl-CoA (Bcd (FN1424, FN0783, and FN1535) to yield Butyryl-CoA; from Butyryl-CoA through the butyryl-CoA, the acetate CoA transferase (But: atoD/atoA) route (FN1856 and FN1857) finally yielded butyrate, and almost...”
- “...31 ] Lysine FN1868 (AAL93967) 3-keto-5-aminohexanoate cleavage enzyme (kce) [ 45 ] Pyruvate FN1421 (AAL95614) FN1020 (AAL95216) Pyruvate-flavodoxin/ferredoxin oxidoreductase (Por/nifJ) Enoyl-Coenzyme A (CoA) hydratase (Crt) This study F. prausnitzii L2-6 Glutarate FP2_05280 (CBK98162) Glutaconyl-CoA decarboxylase beta subunit (GcdB) This study Pyruvate FP2_19990 (CBK99399) FP2_20590 (CBK99451) Pyruvate:ferredoxin...”
- Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586
Kapatral, Journal of bacteriology 2002 - “...deamidation of glutamate by NAD() glutamate dehydrogenase (FN1020), followed by the reduction of 2-oxoglutarate by 2-hydroxyglutarate dehydrogenase. An ORF...”
BCE_4651 enoyl-CoA hydratase/isomerase family protein from Bacillus cereus ATCC 10987
27% identity, 94% coverage
H16_B0915 Enoyl-CoA hydratase/carnithine racemase from Ralstonia eutropha H16
32% identity, 97% coverage
BDBG_02226 enoyl-CoA hydratase from Blastomyces gilchristii SLH14081
28% identity, 88% coverage
- SREB, a GATA transcription factor that directs disparate fates in Blastomyces dermatitidis including morphogenesis and siderophore biosynthesis
Gauthier, PLoS pathogens 2010 - “...of the iron-upregulated genes from A. fumigatus (BDBG_00046, BDBG_00047, BDBG_00048, BDBG_00050, BDBG_00053, BDBG_00054, BDBG_00055, BDBG_01314, BDBG_02226, BDBG_06775, BDBG_06965, BDBG_08034, BDBG_08208, BDBG_09322) and searched the 1 kb upstream for common motifs; MEME options were set for any number of motifs per region (-mod anr), the above described...”
C1G2P3 Enoyl-CoA hydratase from Paracoccidioides brasiliensis (strain Pb18)
PADG_01209 uncharacterized protein from Paracoccidioides brasiliensis Pb18
29% identity, 82% coverage
- Extracellular vesicles from virulent P. brasiliensis induce TLR4 and dectin-1 expression in innate cells and promote enhanced Th1/Th17 response
Montanari, Virulence 2024 - “...(8S) 8,920571 0,020855 C1GL50 NADPH2:quinone reductase UP(D) 6,671233 5,33E05 C1GBI8 Ribose-5-phosphate isomerase UP(D) 5,144232 3,44E05 C1G2P3 Enoyl-CoA hydratase UP(D) 4,382212 3,44E05 C1G977 Hexokinase UP (12S) 4,342947 0,032162 C1GCI8 2-methylcitrate synthase, mitochondrial UP(D) 3,832717 4,39E05 C1GL12 Glycogen debranching enzyme UP(D) 2,979608 0,000124 Amino acid metabolism C1G3Q0 Methylthioribulose-1-phosphate...”
- Transcriptional profiling of a fungal granuloma reveals a low metabolic activity of Paracoccidioides brasiliensis yeasts and an actively regulated host immune response
Borges, Frontiers in cellular and infection microbiology 2023 - “...DOWN(12) -2,42964 0,026103 Energy metabolism C1G294 succinyl-CoA synthetase alpha subunit [EC:6.2.1.4 6.2.1.5] DOWN(D) -3,29572 0,003605 C1G2P3 enoyl-CoA hydratase [EC:4.2.1.17] DOWN(D) -4,37849 2,82E-05 C1G2W2 Pyruvate kinase (EC 2.7.1.40) DOWN(D) -5,32367 0,00092 C1GM03 Cytochrome b-c1 complex subunit 2 DOWN(D) -4,10832 0,000135 Transport C1FZ88 importin subunit alpha-6/7 DOWN(8) -4,36192...”
- “...production at substrate level, and to provide precursors for porphyrin and heme biosynthesis; enoyl-CoA hydratase (C1G2P3) for beta-oxidation, that provide acetyl-CoA to citric acid cycle, but also intermediates for the metabolism of lipids, cholesterol and ketone body; and, Aspartate aminotransferase (C1G3V5), that catalyses the interconversion of...”
- Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
Rosa, Journal of fungi (Basel, Switzerland) 2020 - “...Dihydrolopiol dehydrogenase PAAG_06494 0.0 100% 97% Dolichol-phosphate mannosyltransferase PAAG_01874 0.0 98% 100% Enoyl coenzyme crontonase PADG_01209 0.0 100% 97% Formamidase PAAG_03333 0.0 99% 98% Formamidase PADG_06490 0.0 100% 97% Fumarylacetoacetoato hydrolase PAAG_00869 0.0 98% 100% Glutamyl transferase range PADG_01479 0.0 97% 100% Glutamate dehydrogenase PADG_04516 0.0...”
- Beyond Melanin: Proteomics Reveals Virulence-Related Proteins in Paracoccidioides brasiliensis and Paracoccidioides lutzii Yeast Cells Grown in the Presence of L-Dihydroxyphenylalanine
Almeida-Paes, Journal of fungi (Basel, Switzerland) 2020 - “...the role of L-DOPA in the virulence enhancement of this species. The enzymes enoyl-CoA hydratase (PADG_01209) and acyl-CoA dehydrogenase (PADG_02244) were more abundant in melanized Pb 18, indicating -oxidation of fatty acids in this strain, which also occurs when acetate is available for fungal growth [...”
- Proteomic Analysis of Paracoccidioides brasiliensis During Infection of Alveolar Macrophages Primed or Not by Interferon-Gamma
Chaves, Frontiers in microbiology 2019 - “...F1, gamma subunit; PADG_08349, ATP synthase subunit beta, mitochondrial; PADG_07789, ATP synthase subunit delta, mitochondrial; PADG_01209, enoyl-CoA hydratase; PADG_03194, 3-ketoacyl-CoA thiolase B; PADG_01687, 3-ketoacyl-CoA thiolase; PADG_03449, isopentenyl-diphosphate delta-isomerase; PADG_04343, short chain dehydrogenase/reductase; PADG_02751, acetyl-CoA acetyltransferase. Proteins Regulated in P. brasiliensis During Infection of Non-primed Macrophages A...”
- Differential Metabolism of a Two-Carbon Substrate by Members of the Paracoccidioides Genus
Baeza, Frontiers in microbiology 2017 - “...and Pb EPM83 (PAAG_06329; PAAG_02664; PAAG_05454; PAAG_03116; PAAG_06309; PAAG_06392; PAAG_01557; PADG_01228; PADG_01687; PADG_06805; PADG_07023; PADG_06721; PADG_01209; PADG_02527), producing acetyl-CoA and propionyl-CoA. Acetyl-CoA can be consumed in the glyoxylate cycle for biosynthetic purposes or in the TCA cycle to generate cellular energy as reduced cofactors. Beta-oxidation was...”
- “...01 because of up-regulation of the enzymes acylCoA dehydrogenase (PAAG_05454; PADG_06805) and enoyl-CoA hydratase (PAAG_06309; PADG_01209) in both (Figure 7 , Supplementary Figure 8 ), as well as the up-regulation of 2-oxovalerate dehydrogenase (PAAG_01194) and methylmalonate-semialdehyde dehydrogenase (PAAG_07036) in Pb 01 and 3-hydroxyisobutyrate dehydrogenase (PADG_03466) and...”
- Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection
Lacerda, Virulence 2017 - “...ethanol production (TableS4). Proteins involved with fatty acid breakdown, acyl-CoA-binding protein (PADG_01363), enoyl CoA hydratase (PADG_01209), and isocitrate lyase (PADG_01483) in the glyoxylate cycle was induced 1.53-fold in vivo . Enzymes involved with ethanol production are upregulated; alcohol dehydrogenase (PADG_04701) and pyruvate decarboxylase (PADG_00714) increased 13.73...”
- “...MDH malate dehydrogenase (PADG_08054), ICL isocitrate lyase (PADG_01483), MLS malate synthase (PADG_04702), ECH enoyl-CoA hydratase (PADG_01209), ACD acyl-CoA dehydrogenase (PADG_07604) PLA phospholipase (PADG_05993), PGL phosphoglucolactonase (PADG_07771), TRX thioredoxins (PADG_03161 and PADG_05504), TrxR thioredoxin reductase (PADG_01551), SOD superoxide dismutase (PADG_07418 and PADG_01755), CCP cytochrome C peroxidase (PADG_03163)....”
- Macrophage Interaction with Paracoccidioides brasiliensis Yeast Cells Modulates Fungal Metabolism and Generates a Response to Oxidative Stress
Parente-Rocha, PloS one 2015 - “...subunit 120.14 * PADG_08013 Succinate dehydrogenase iron sulfur subunit 665.89 * Beta-oxidation of fatty acid PADG_01209 Enoyl CoA hydratase 11615.45 1.84 Amino acid degradation PADG_03020 Alanine glyoxylate aminotransferase 1597.6 1.93 PADG_01621 Aspartate aminotransferase 768.6 * PADG_08468 4-hydroxyphenylpyruvate dioxygenase 9595.33 2.10 PADG_02214 4-aminobutyrate aminotransferase 1086.24 1.79 PADG_04516...”
ANACAC_00255 hypothetical protein from Anaerostipes caccae DSM 14662
30% identity, 96% coverage
BAS4420 enoyl-CoA hydratase/isomerase family protein from Bacillus anthracis str. Sterne
27% identity, 94% coverage
NP_629596 enoyl-coA hydratase from Streptomyces coelicolor A3(2)
31% identity, 86% coverage
- Distribution analysis of hydrogenases in surface waters of marine and freshwater environments
Barz, PloS one 2010 - “...YP_583693; Saverm, Streptomyces avermitilis MA-4680 NP_828541; Savermi Streptomyces avermitilis MA-4680 NP_823962; Scoelic Streptomyces coelicolor A3(2) NP_629596; Sdegra, Saccharophagus degradans 2-40 YP_526001; Smalto Stenotrophomonas maltophilia R551-3 YP_002027502; Ssedimi, Shewanella sediminis HAW-EB3 YP_001475080; Sulfuro, Sulfurovum sp. NBC37-1 YP_001358952; Xcamp Xanthomonas campestris pv. vesicatoria str. 85-10 YP_363011 (0.50 MB...”
Q39TX4 Enoyl-CoA hydratase/isomerase from Geobacter metallireducens (strain ATCC 53774 / DSM 7210 / GS-15)
Gmet_2071 Enoyl-CoA hydratase/isomerase from Geobacter metallireducens GS-15
29% identity, 92% coverage
- Adaptation of Carbon Source Utilization Patterns of Geobacter metallireducens During Sessile Growth
Marozava, Frontiers in microbiology 2020 - “...release ABC transporter, membrane protein 0.1 1.8 1.8 Butanoate metabolism Q39TU7 Phosphotransbutyrylase 1.5 0.4 0.4 Q39TX4 Enoyl-CoA hydratase/isomerase 1.4 0.4 0.2 Q39UY1 Electron transfer flavoprotein, alpha subunit 0.9 4.5 2.6 Q39UY5 Acyl-CoAcarboxylate coenzyme A transferase, beta subunit 0.7 0.5 0.2 Ethanol degradation Q39WT9 Aldehyde:ferredoxin oxidoreductase, tungsten-containing...”
- Genomic and microarray analysis of aromatics degradation in Geobacter metallireducens and comparison to a Geobacter isolate from a contaminated field site
Butler, BMC genomics 2007 - “...Bacterial regulatory proteins, IclR Gmet_2065 28.7 oxr 4Fe-4S binding Gmet_2068 32.6 scsA* Succinyl-CoA synthetase, alpha Gmet_2071 14.1 ech* Enoyl-CoA hydratase/isomerase Gmet_2072 18.4 acd^ 3-hydroxyacyl-CoA dehydrogenase Gmet_2074 50.7 bamN^ Thiolase Gmet_2075 19.1 bamM* Acyl-CoA dehydrogenase Gmet_2077 21.8 bamK N-acetyltransferase Gmet_2080 36.1 bamH^ NAD(P) diaphorase, HoxF Gmet_2081 27.5...”
- “...15-fold were another thiolase (Gmet_2058), two acyl-CoA dehydrogenases (Gmet_2072, Gmet_2075), and two enoyl-CoA hydratases (Gmet_2057, Gmet_2071) (Table 2 ). Thus, though there are several homologs to beta fatty acid oxidation genes in the genome, only one cluster showed increases in expression during benzoate oxidation (Figure 5...”
6slbAAA / H9ZNW0 6slbAAA (see paper)
32% identity, 97% coverage
- Ligand: (~{e})-6-[2-[3-[[(2~{r})-4-[[[(2~{r},3~{s},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid (6slbAAA)
3p85A / A0A0H2ZUQ2 Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
34% identity, 79% coverage
- Ligand: calcium ion (3p85A)
Slip_2089 enoyl-CoA hydratase-related protein from Syntrophothermus lipocalidus DSM 12680
27% identity, 92% coverage
- Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Liu, mSphere 2021 - “...Catalytic properties of 3-hydroxypropionyl-CoA dehydrases/crotonyl-CoA hydratases Msed_2001 and Nmar_1308 as well as homologous enoyl-CoA hydratase Slip_2089 from S. lipocalidus d Substrate Msed_2001 Nmar_1308 Slip_2089 V max (Umg 1 protein) K m (mM) k cat / K m (s 1 mM 1 ) a V max (Umg...”
- “...catalytic properties of one of those homologs, i.e., the enoyl-CoA hydratase homolog from Syntrophothermus lipocalidus Slip_2089, were studied. FIG3 Maximum likelihood phylogenetic tree of 3-hydroxypropionyl-CoA dehydratases/crotonyl-CoA hydratases. Msed_2001, Nmar_1308, and Slip_2089 are marked with *. Bacterial sequences are shown in green, euryarchaeal in black, the sequences...”
Q9HYH9 Probable enoyl CoA-hydratase/isomerase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA3426 enoyl-CoA hydratase from Pseudomonas aeruginosa PAO1
35% identity, 68% coverage
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at least 83 amino acids, distances of 200 Pam units or less, and aligned over at least...”
- Dissection of the cis-2-decenoic acid signaling network in Pseudomonas aeruginosa using microarray technique
Rahmani-Badi, Frontiers in microbiology 2015 - “...PA1240, PA1470, PA1535, PA1576, PA1628-PA1629, PA1631, PA1827, PA1869, PA2550, PA2552, PA2815, PA2841, PA2887-PA2891, PA2893, PA3286, PA3426, PA3589, PA3591, PA3593, PA3924,PA4089, PA4330, PA4912, PA4979-PA4980, PA4995, PA5020, PA5524 Protein and Amino acid metabolism thrS, folC, glnA, gmk, tgt, dadA, pauA3A5, gltX, gcvT1T2, glyQ, gdhA, valS, purD, trmD, speA,...”
- “...microarray analysis showed that 8 genes encoding putative enoyl-CoA hydratase/isomerase ( PA3591, PA1021, PA1629, PA2890, PA3426, PA4330, PA1240 , and PA2841 ) were highly up-regulated in our study, known PPI data showed that only PA4980 directly interacts with a sensor kinase and its response regulator (Yang...”
Rv2831 enoyl-CoA hydratase from Mycobacterium tuberculosis H37Rv
I6YEH6 Probable enoyl-CoA hydratase EchA16 (Enoyl hydrase) (Unsaturated acyl-CoA hydratase) (Crotonase) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
BCG_2851 putative enoyl-CoA hydratase echa16 from Mycobacterium bovis BCG str. Pasteur 1173P2
36% identity, 79% coverage
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...0.020180231 BCG_3851 BCG_3851 Rv3789 Conserved hypothetical protein with GtrA domain-containing protein 0.6412 0.02717627 echA16 BCG_2851 Rv2831 Probable enoyl-CoA hydratase echa16 0.6407 0.040951984 BCG_3745c BCG_3745c Rv3686c Conserved hypothetical protein 0.6383 0.023684945 BCG_0241c BCG_0241c Rv0204c Probable conserved transmembrane protein 0.6362 0.010034117 BCG_3015 BCG_3015 Rv2994 Probable conserved integral membrane...”
- Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress
Lu, Microbiology spectrum 2023 - “...Interestingly, the modification levels of FadA ( Rv0859 ), EchA7 ( Rv0971c ), EchA16 ( Rv2831 ), and EchA19 ( Rv3516 ) were all increased, which were involved in fatty acid metabolism, valine, leucine and isoleucine degradation, lysine degradation, fatty acid degradation, tryptophan metabolism, benzoate degradation,...”
- “...( O53871 , Rv0859 ), enoyl-CoA hydratase EchA7 (P71540, Rv0971c ), enoyl-CoA hydratase EchA16 (I6YEH6, Rv2831 ), oxidoreductase ( P9WGQ9 , Rv0769 ), thiamine biosynthesis oxidoreductase ThiO (P96261, Rv0415 ), stress-related carboxylesterase B CaeB ( P9WHR5 , Rv2223c ), PSase PhyA ( P9WHP3 , Rv3397c ),...”
- Immunogenicity of Mycobacterial Extracellular Vesicles Isolated From Host-Related Conditions Informs About Tuberculosis Disease Status
Schirmer, Frontiers in microbiology 2022 - “...DnaK 0.32 Rv0432 SodC 0.39 Lipid metabolism 110 Rv0242c FabG4 0.83 35 Rv0242c FabG4 1.39 Rv2831 EchA16 0.59 Rv2244 AcpM 1.2 Rv0824c DesA1 0.56 Rv2831 EchA16 1.08 Rv3800c Pks13 0.54 Rv0675 EchA5 1.07 Rv1070c EchA8 0.51 Rv0642c MmaA4 0.72 Information pathways 93 Rv0723 RpIO 1.18 41...”
- TB or not to be: what specificities and impact do antibodies have during tuberculosis?
Hermann, Oxford open immunology 2021 - “...hypothetical protein x Rv0983 Probable serine protease PepD x Rv0474 Probable transcriptional regulatory protein x Rv2831 Probable enoyl-CoA hydratase EchA16 x Rv3912 Hypothetical alanine-rich protein x Rv1904 Conserved hypothetical protein x Rv2922c Probable chromosome partition protein Smc x Rv2668 Possible exported alanine and valine rich protein...”
- VapBC22 toxin-antitoxin system from Mycobacterium tuberculosis is required for pathogenesis and modulation of host immune response
Agarwal, Science advances 2020 - “...( 29 , 30 ). We also noticed that the levels of Rv2830c (VapB22) and Rv2831 (EchA16) proteins were significantly increased in the mutant strain ( Fig. 3A and table S3). Among proteins with elevated expression levels, Rv3477 (PE31), Rv3804c (FbpA), Rv3615c (EspC), Rv3616c (EspA), Rv3478...”
- Identification of novel mutations associated with cycloserine resistance in Mycobacterium tuberculosis
Chen, The Journal of antimicrobial chemotherapy 2017 - “...Gene alr rv0759c rv1403c rv1435c rv1726 rv1731 rv2831 Materials and methods D-Cycloserine-resistant mutant isolation M. tuberculosis H37Rv cultures, 1 month...”
- “...non-synonymous non-synonymous non-synonymous PE_PGRS50 (rv3345c) rv3690 echA16 (rv2831) hisC2 (rv3772) G(#52)T G103A G253T T110C -- D35N A85S L37P alr...”
- Using a Label Free Quantitative Proteomics Approach to Identify Changes in Protein Abundance in Multidrug-Resistant Mycobacterium tuberculosis
Phong, Indian journal of microbiology 2015 - “...4.5 Rv1912c Possible oxidoreductase fadB5 1 5.9 Rv2831 Rv1080c Enoyl-CoA hydratase/isomerase family protein Transcription elongation factor echA16 greA 1 2 6.2...”
- “...FadB5 (Rv1912) and enoyl-CoA hydratase/isomerase family protein (Rv2831). Rv2187 catalyzes the activation of long-chain fatty acids as acyl-coenzyme A...”
- Flux Balance Analysis with Objective Function Defined by Proteomics Data-Metabolism of Mycobacterium tuberculosis Exposed to Mefloquine
Montezano, PloS one 2015 - “...Rv2868c I6YEL0 30 Day 2 Rv0155 P96832 44 Day 2 Rv1001 I6X008 29 Day 4 Rv2831 I6YEH6 37 Day 4 Rv0382c I6Y3M7 29 Day 4 Rv0500 I6Y7Z2 34 Day 4 Rv0753c 053816 34 This table shows proteins with largest fold-change values between mefloquine and control experimental...”
- More
- Identification and Quantification of S-Sulfenylation Proteome of Mycobacterium tuberculosis under Oxidative Stress
Lu, Microbiology spectrum 2023 - “...FadA ( O53871 , Rv0859 ), enoyl-CoA hydratase EchA7 (P71540, Rv0971c ), enoyl-CoA hydratase EchA16 (I6YEH6, Rv2831 ), oxidoreductase ( P9WGQ9 , Rv0769 ), thiamine biosynthesis oxidoreductase ThiO (P96261, Rv0415 ), stress-related carboxylesterase B CaeB ( P9WHR5 , Rv2223c ), PSase PhyA ( P9WHP3 , Rv3397c...”
- Flux Balance Analysis with Objective Function Defined by Proteomics Data-Metabolism of Mycobacterium tuberculosis Exposed to Mefloquine
Montezano, PloS one 2015 - “...I6YEL0 30 Day 2 Rv0155 P96832 44 Day 2 Rv1001 I6X008 29 Day 4 Rv2831 I6YEH6 37 Day 4 Rv0382c I6Y3M7 29 Day 4 Rv0500 I6Y7Z2 34 Day 4 Rv0753c 053816 34 This table shows proteins with largest fold-change values between mefloquine and control experimental conditions....”
- Comparison of the transcriptome, lipidome, and c-di-GMP production between BCGΔBCG1419c and BCG, with Mincle- and Myd88-dependent induction of proinflammatory cytokines in murine macrophages
Flores-Valdez, Scientific reports 2024 - “...0.6448 0.020180231 BCG_3851 BCG_3851 Rv3789 Conserved hypothetical protein with GtrA domain-containing protein 0.6412 0.02717627 echA16 BCG_2851 Rv2831 Probable enoyl-CoA hydratase echa16 0.6407 0.040951984 BCG_3745c BCG_3745c Rv3686c Conserved hypothetical protein 0.6383 0.023684945 BCG_0241c BCG_0241c Rv0204c Probable conserved transmembrane protein 0.6362 0.010034117 BCG_3015 BCG_3015 Rv2994 Probable conserved integral...”
BB341_RS07030, SCLAV_4367 enoyl-CoA hydratase/isomerase family protein from Streptomyces clavuligerus
33% identity, 85% coverage
- The CagRS Two-Component System Regulates Clavulanic Acid Metabolism via Multiple Pathways in Streptomyces clavuligerus F613-1
Fu, Frontiers in microbiology 2019 - “...system affects CA biosynthesis ( Santamarta et al., 2005 ); pgk , encoding phosphoglycerate kinase; BB341_RS07030, BB341_RS26625 and BB341_RS03665 ( paaH ), which are involved in fatty acid degradation; and BB341_RS20995 ( glnA3 ), a gene encoding glutamine synthetase, which is involved in arginine biosynthesis. Moreover,...”
- “...biosynthesis protein CA biosynthesis regulation 2.53812 SCLAV_1147 (pgk) BB341_RS22330 (pgk) Phosphoglycerate kinase Glycolysis 3.0029 SCLAV_4367 BB341_RS07030 Enoyl-CoA hydratase Fatty acid degradation 2.33484 SCLAV_0228 BB341_RS26625 Acyl-CoA dehydrogenase Fatty acid degradation 3.83473 SCLAV_2974(paaH) BB341_RS03665(paaH) 3-hydroxybutyryl-CoA dehydrogenase Fatty acid degradation 4.78544 SCLAV_1431(glnA3) BB341_RS20995(glnA3) Glutamine synthetase Arginine synthesis 2.80523 The...”
- “...Gamma-butyrolactone biosynthesis protein CA biosynthesis regulation 2.53812 SCLAV_1147 (pgk) BB341_RS22330 (pgk) Phosphoglycerate kinase Glycolysis 3.0029 SCLAV_4367 BB341_RS07030 Enoyl-CoA hydratase Fatty acid degradation 2.33484 SCLAV_0228 BB341_RS26625 Acyl-CoA dehydrogenase Fatty acid degradation 3.83473 SCLAV_2974(paaH) BB341_RS03665(paaH) 3-hydroxybutyryl-CoA dehydrogenase Fatty acid degradation 4.78544 SCLAV_1431(glnA3) BB341_RS20995(glnA3) Glutamine synthetase Arginine synthesis 2.80523...”
LIC_12495 enoyl-CoA hydratase-related protein from Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130
24% identity, 95% coverage
PAAG_06309 enoyl-CoA hydratase from Paracoccidioides lutzii Pb01
29% identity, 82% coverage
- The Response of Paracoccidioides lutzii to the Interaction with Human Neutrophils
Silva, Journal of fungi (Basel, Switzerland) 2023 - “...related to fatty acids oxidation, such as 3 hydroxybutyryl CoA dehydrogenase (PAAG_06329), enoyl CoA hydratase (PAAG_06309) and 3 ketoacyl CoA thiolase (PAAG_02664), were also increased after neutrophil internalization, suggesting a potential utilization of fatty acids as fuel within neutrophils. The interaction with neutrophils also increased the...”
- “...0.68 ** PAAG_08859 peroxisomal multifunctional enzyme 1.43 * PAAG_06329 3 hydroxybutyryl CoA dehydrogenase # 1.12 PAAG_06309 enoyl CoA hydratase 1.27 1.32 PAAG_02664 3 ketoacyl CoA thiolase # 1.62 PAAG_01310 2-oxoisovalerate dehydrogenase subunit alpha # 1.65 PAAG_05984 glutaryl CoA dehydrogenase # 1.38 PAAG_03330 dihydrolipoyl dehydrogenase # 1.26...”
- Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
Rosa, Journal of fungi (Basel, Switzerland) 2020 - “...dehydrogenase PADG_06805 0.0 100% 98% Acyl CoA dehydrogenase PADG_07604 0.0 100% 97% Acyl CoA hydratase PAAG_06309 0.0 97% 100% Actin F protein subunit uptake protein PADG_07756 0.0 100% 100% Alcohol dehydrogenase PADG_01174 0.0 100% 97% Alpha-1,2 mannosyltransferase PAAG_02462 0.0 100% 98% Alpha-1,2 mannosyltransferase KTR1 PAAG_07238 0.0...”
- Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses
Santos, IMA fungus 2020 - “...act as acyl-CoA dehydrogenases and acyl-CoA hydratase were found as induced in our work (PAAG_06329, PAAG_06309, PAAG_04811). However, further efforts need to be done in order to confirm this hypothesis. CONCLUSIONS Our study brought new insights into biochemical and molecular aspects of propionate metabolism in Paracoccidioides...”
- Differential Metabolism of a Two-Carbon Substrate by Members of the Paracoccidioides Genus
Baeza, Frontiers in microbiology 2017 - “...of fatty acids mainly functions in Pb 01 and Pb EPM83 (PAAG_06329; PAAG_02664; PAAG_05454; PAAG_03116; PAAG_06309; PAAG_06392; PAAG_01557; PADG_01228; PADG_01687; PADG_06805; PADG_07023; PADG_06721; PADG_01209; PADG_02527), producing acetyl-CoA and propionyl-CoA. Acetyl-CoA can be consumed in the glyoxylate cycle for biosynthetic purposes or in the TCA cycle to...”
- “...Pb 01 because of up-regulation of the enzymes acylCoA dehydrogenase (PAAG_05454; PADG_06805) and enoyl-CoA hydratase (PAAG_06309; PADG_01209) in both (Figure 7 , Supplementary Figure 8 ), as well as the up-regulation of 2-oxovalerate dehydrogenase (PAAG_01194) and methylmalonate-semialdehyde dehydrogenase (PAAG_07036) in Pb 01 and 3-hydroxyisobutyrate dehydrogenase (PADG_03466)...”
- Proteomic profile response of Paracoccidioides lutzii to the antifungal argentilactone
Prado, Frontiers in microbiology 2015 - “...* Methyl citrate cycle 2-methylcitrate dehydratase PAAG_04559 20407.15 1.297 Oxidation of fatty acids Enoyl-CoA hydratase PAAG_06309 3244.11 1.716 Acetyl-CoA acetyltransferase PAAG_03447 1578.04 * Peroxisomal 3-ketoacyl-coA thiolase PAAG_03689 1248.76 * Siderophore-iron transport Siderophore peptide synthase PAAG_02354 1582.68 * Protein fate Chaperone DnaK PAAG_01339 14261.80 1.259 Chaperonin PAAG_05142...”
SE37_11185 enoyl-CoA hydratase-related protein from Geobacter soli
29% identity, 95% coverage
- Genome sequence of a dissimilatory Fe(III)-reducing bacterium Geobacter soli type strain GSS01(T)
Yang, Standards in genomic sciences 2015 - “...G. bemidjiensis ; SE37_13685), acyl-CoA thioesterases (SE37_09325, SE37_09950, SE37_10860, SE37_14445 and SE37_15385), enoyl-CoA hydratases (SE37_15375; SE37_11185, 81% similarity to Gmet_1716 in G. metallireducens ), phenylacetate-CoA ligase (SE37_04405, SE37_06045 and SE37_06085) and acyl-CoA synthetase (SE37_06810). The ability to utilize aromatic compounds and other carbon sources may be...”
Q5SLK3 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) from Thermus thermophilus (see paper)
32% identity, 96% coverage
SS1G_04249 hypothetical protein from Sclerotinia sclerotiorum 1980 UF-70
26% identity, 80% coverage
XNRR2_0150 enoyl-CoA hydratase from Streptomyces albidoflavus
30% identity, 98% coverage
3pe8A / A0QVP0 Crystal structure of enoyl-coa hydratase from mycobacterium smegmatis (see paper)
32% identity, 74% coverage
XP_059733516 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial from Bos taurus
25% identity, 76% coverage
SIDH_BEAB2 / J4KLY0 Mevalonyl-coenzyme A hydratase SIDH; Siderophore biosynthesis cluster protein H; EC 4.2.1.- from Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus) (Tritirachium shiotae) (see paper)
31% identity, 88% coverage
- function: Mevalonyl-coenzyme A hydratase; part of the gene cluster that mediates the biosynthesis of at least 11 siderophores, including beauverichelin A, dimerumic acid (DA), Na-dimethyl coprogen (NADC), eleutherazine B, ferricrocin (FC), fusarinine A, fusarinine C (FsC), metachelin A, mevalonolactone, rhodotorulic acid (RA) and tenellin (PubMed:39109629). This cocktail of siderophores for iron metabolism is essential for virulence, and more specifically for the fungal virulence in penetrating through the host cuticle (PubMed:39109629). Siderophore synthesis is also involved in conidial germination under iron-deficient conditions (PubMed:39109629). For biosynthesis of fusarinine C, the transacylase SIDF transfers anhydromevalonyl to N(5)-hydroxyornithine. The required anhydromevalonyl-CoA moiety is derived from mevalonate by CoA ligation and dehydration catalyzed by SIDI and sidH respectively (By similarity). SIDH is not essential for siderophore production, probably due to functional redundancy of this protein family, as there are 15 homologs of SIDH in B.bassiana (PubMed:39109629).
disruption phenotype: Does not affect conidial germination, sensitivity to oxidative stress and iron-starvation, nor virulence.
G7JC24 Enoyl-CoA hydratase/delta3,5-delta2,4-dienoyl-CoA isomerase from Medicago truncatula
28% identity, 91% coverage
PAAG_07013 enoyl-CoA hydratase/carnithine racemase from Paracoccidioides lutzii Pb01
26% identity, 88% coverage
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...* porphyrin biosynthesis Lipid, fatty acid and isoprenoid metabolism PAAG_02211 GDSL Lipase/Acylhydrolase * lipid metabolism PAAG_07013 enoyl-CoA hydratase/carnithine racemase * lipid, fatty acid and isoprenoid metabolism PAAG_01525 fatty acid synthase subunit alpha reductase * lipid and fatty acid biosynthesis PAAG_01524 fatty acid synthase subunit beta dehydratase...”
Igni_1058 3-hydroxyacyl-CoA dehydrogenase, NAD-binding from Ignicoccus hospitalis KIN4/I
28% identity, 39% coverage
- Functional compartmentalization and metabolic separation in a prokaryotic cell
Flechsler, Proceedings of the National Academy of Sciences of the United States of America 2021 (secret) - (S)-3-Hydroxybutyryl-CoA Dehydrogenase From the Autotrophic 3-Hydroxypropionate/4-Hydroxybutyrate Cycle in Nitrosopumilus maritimus
Liu, Frontiers in microbiology 2021 - “...al., 2011 ; Hawkins et al., 2014 ; Liu et al., 2020 Ignicoccus hospitalis CCH/HBDH Igni_1058 117/152 0.086 1,800 NR NR NR Flechsler et al., 2021 Cupriavidus necator H16 HBDH/enoyl-CoA hydratase b H16_A0461 NR NR NR 149/216 0.048 4,500 Volodina and Steinbchel, 2014 HBDH RePaaH1 NR...”
- “...red triangle, Msed_1423; red star, Msed_0399; olive star, Tneu_0541 (from Pyrobaculum neutrophilum ); turquoise star, Igni_1058 (from Ignicoccus hospitalis ); , dehydrogenase; without , fusion protein; 80 sequences from TACK group in red, 15 sequences from non-AOA Thaumarchaeota in fuchsia, 49 sequences from Euryarchaeota in blue,...”
- Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota
Ramos-Vera, Journal of bacteriology 2011 - “...(N-terminal domain, 40 kDa) (Msed_0399, Tneu_0541, Igni_1058). Autotrophic Sulfolobales contained additional, less similar candidate genes coding for members...”
- The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota
Siebers, PloS one 2011 - “...4-Hydroxybutyryl-CoA dehydratase TTX_1102 Tneu_0422 (100%, 0.0) Crotonyl-CoA hydratase/( S )-3-Hydroxybutyryl-CoA DH TTX_1028 Tneu_0541 (99%, 0.0) Igni_1058 (99%, 1e-160) Acetoacetyl-CoA -ketothiolase TTX_0886 Tneu_0249 (99%, 1e-166) Igni_1401 (99%, 2e-104) The corresponding e-values derived from blastp analyses of the T. neutrophilus (Tneu_) and I. hospitalis (Igni_) candidates involved in...”
N646_4048 enoyl-CoA hydratase-related protein from Vibrio alginolyticus NBRC 15630 = ATCC 17749
27% identity, 92% coverage
ELI_0538, KR505_10040 enoyl-CoA hydratase-related protein from Eubacterium callanderi
29% identity, 94% coverage
- Biosynthesis of butyrate from methanol and carbon monoxide by recombinant Acetobacterium woodii
Chowdhury, International microbiology : the official journal of the Spanish Society for Microbiology 2022 - “...KIST612 harbors the genes encoding thiolase ( thlA , ELI_0537), 3-hydroxybutyryl-CoA dehydrogenase ( hbd , ELI_0538), crotonase ( crt , ELI_0539), and the electron bifurcating butyryl-CoA dehydrogenase complex ( bcd/etfAB , ELI_0540-0542). While the genes of the butyrate pathway are clustered in a single gene cluster,...”
- Gut Microbiota Eubacterium callanderi Exerts Anti-Colorectal Cancer Activity
Ryu, Microbiology spectrum 2022 - “...electron transfer flavoprotein subunit beta (KR505_10025); Hbd , hydroxybutyryl dehydrogenase (KR505_10035); Cro , crotonase/enoyl-CoA hydratase (KR505_10040); Thl , acetyl-CoA C-acetyltransferase (KR505_10045). (B) Series of genes related to GABA biosynthesis. GadC , glutamate/gamma-aminobutyrate antiporter (KR505_12475); GadB , glutamate decarboxylase (KR505_12480); GdhA , glutamate dehydrogenase (NADP + )...”
- Energy Conservation Model Based on Genomic and Experimental Analyses of a Carbon Monoxide-Utilizing, Butyrate-Forming Acetogen, Eubacterium limosum KIST612
Jeong, Applied and environmental microbiology 2015 - “...(thlA, ELI_0537), 3-hydroxybutyryl-CoA dehydrogenase (hbd, ELI_0538), crotonase (crt, ELI_0539), and butyryl-CoA dehydrogenase (bcd, ELI_0540) (Fig. 6A)....”
XP_001551006 hypothetical protein from Botrytis cinerea B05.10
27% identity, 89% coverage
- Fungal siderophore biosynthesis is partially localized in peroxisomes
Gründlinger, Molecular microbiology 2013 - “...XP_002484634 RLQQTQRHI XP_002486020.1 SKL 8.4 XP_002486021.1 AKL 6.9 Leotiomycetes Botryotinia fuckeliana XP_001546797.1 PKL a 13.5 XP_001551006 SKL 9.1 XP_001551005.1 PKL a 6.5 Sclerotinia sclerotiorum XP_001585101.1 PKL 12.7 XP_001594442.1 SKL b 9.1 XP_001594441.1 AKL 6.9 Sordariomycetes Neurospora crassa XP_959826.1 SKL 6.2 XP_962600.1 SKL 6.3 XP_959825.1 PKL 6.5...”
Q9I5I4 enoyl-CoA hydratase (EC 4.2.1.17) from Pseudomonas aeruginosa (see paper)
PA14_54640 probable enoyl-CoA hydratase/isomerase from Pseudomonas aeruginosa UCBPP-PA14
PA0745 enoyl-CoA hydratase from Pseudomonas aeruginosa PAO1
29% identity, 94% coverage
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Evolution by leaps: gene duplication in bacteria
Serres, Biology direct 2009 - “...P52045, P77467, P76082, Q9I498, Q9I002, Q9I393, Q9HY35, Q9HZJ2, Q9I300, Q9HZV7, Q9I298, Q9I5I5, Q9HW71, Q9HUI5, Q9I2S4, Q9I5I4, Q9I4V3, Q9I2Y9, Q9I076, Q9HYH9, Q9L6L5, Q8ZNA7, Q82RX5, Q7CQ56). Sequence pairs were collected that had alignment lengths of at least 83 amino acids, distances of 200 Pam units or less, and...”
- <i>cis</i>-DA-dependent dispersion by <i>Pseudomonas aeruginosa</i> biofilm and identification of <i>cis</i>-DA-sensory protein DspS
Kalia, mBio 2023 - “...). Production of cis -DA by P. aeruginosa requires an enoyl-CoA synthetase encoded by dspI (PA14_54640, a PA0745 ortholog), with dspI inactivation resulting in significantly reduced dispersion events and defective swarming motility ( 34 ). Transcriptomic profiling indicated that cis -DA affects the expression of 666...”
- “...the enoyl-CoA synthetase necessary for the production of cis -DA, biofilms formed by the dspI (PA14_54640, PA0745) mutant were devoid of central voids. Overall, void formation was observed in only 5% of microcolonies of dspI mutant biofilms compared with 63% of wild-type PA14 biofilm microcolonies (...”
- Pseudomonas aeruginosa core metabolism exerts a widespread growth-independent control on virulence
Panayidou, Scientific reports 2020 - “...virulent strains metabolic genes exhibited clear defects in virulence (Fig. 3B ). Of the latter, PA14_54640 ( dspI ) encodes for a putative enoyl-CoA hydratase involved in biofilm dispersion. Its mutation produces high biofilm formation, a condition that may promote chronicity to the expense of acute...”
- Control of Biofilms with the Fatty Acid Signaling Molecule cis-2-Decenoic Acid
Marques, Pharmaceuticals (Basel, Switzerland) 2015 - “...virulence, iron uptake, and respiration [ 119 ]. Current findings have shown the dspI gene (PA14_54640), a PA0745 ortholog, to be required for the production of cis -DA [ 120 ]. Mutation of dspI in P. aeruginosa leads to a reduction of pyoverdine (a virulence factor)...”
- The putative enoyl-coenzyme A hydratase DspI is required for production of the Pseudomonas aeruginosa biofilm dispersion autoinducer cis-2-decenoic acid
Amari, Journal of bacteriology 2013 - “...pathogen Pseudomonas aeruginosa. The protein is encoded by PA14_54640 (PA0745), named dspI for dispersion inducer. The gene sequence for this protein shows...”
- “...the present work, we report that the gene PA14_54640 (PA0745), named dspI (dispersion inducer), is required for pro- 4600 jb.asm.org Journal of Bacteriology...”
- <i>cis</i>-DA-dependent dispersion by <i>Pseudomonas aeruginosa</i> biofilm and identification of <i>cis</i>-DA-sensory protein DspS
Kalia, mBio 2023 - “...of cis -DA by P. aeruginosa requires an enoyl-CoA synthetase encoded by dspI (PA14_54640, a PA0745 ortholog), with dspI inactivation resulting in significantly reduced dispersion events and defective swarming motility ( 34 ). Transcriptomic profiling indicated that cis -DA affects the expression of 666 genes (...”
- “...enoyl-CoA synthetase necessary for the production of cis -DA, biofilms formed by the dspI (PA14_54640, PA0745) mutant were devoid of central voids. Overall, void formation was observed in only 5% of microcolonies of dspI mutant biofilms compared with 63% of wild-type PA14 biofilm microcolonies ( 34...”
- Identification of genes involved in enhanced membrane vesicle formation in Pseudomonas aeruginosa biofilms: surface sensing facilitates vesiculation
Kanno, Frontiers in microbiology 2023 - “...0.054 (0.024) 1.5 PAMK78G2 PA0744 (812073) Probable enoyl-CoA hydratase/isomerase 0.078 (0.0094) 0.85 (0.12) 1.3 PAMK90H12 PA0745 (813068) dspI Putative enoyl-CoA hydratase/isomerase 0.23 (0.16) 0.78 (0.12) 1.7 PAMK20E7 PA1430 (1558495) lasR Transcriptional regulator 0.24 (0.017) 1.3 (0.33) 1.7 PAMK42F4 PA3479 (3892951) rhlA Rhamnosyltransferase chain A 0.13 (0.028)...”
- The Pseudomonas aeruginosa RpoH (σ32) Regulon and Its Role in Essential Cellular Functions, Starvation Survival, and Antibiotic Tolerance
Williamson, International journal of molecular sciences 2023 - “...PA5001-PA5010. The PA0744 operon includes enzymes for fatty acid biosynthesis, including Enoyl-CoA hydratase/isomerase (PA0744 and PA0745), Acyl-CoA dehydrogenase (PA0746), and NAD-dependent aldehyde dehydrogenase (PA0747). The structure and function of PA0745 (DspI) were determined [ 70 ] and shown to be involved in the dehydration reaction cis-2-decenoic...”
- “...sigma factor RpoD 2.7 2.0 10 3 +++ PA0744 Enoyl-CoA hydratase/isomerase 8.3 3.0 10 4 PA0745 dspI Enoyl-CoA hydratase/isomerase 10.7 6.2 10 4 PA0746 Acyl-CoA dehydrogenase 10.5 1.8 10 3 PA0747 NAD-dependent aldehyde dehydrogenase 14.2 6.3 10 4 PA0763 mucA Anti-sigma factor 2.1 2.5 10 3...”
- The Small RNAs PA2952.1 and PrrH as Regulators of Virulence, Motility, and Iron Metabolism in Pseudomonas aeruginosa
Coleman, Applied and environmental microbiology 2021 (secret) - Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis
Liu, Scientific reports 2018 - “...in DSF biosynthesis 7 , 16 21 . In P . aeruginosa , the gene PA0745 encodes a putative crotonase, named dspI , which has been shown to be responsible for the CDA biosynthesis 10 . Meanwhile, dspI has been verified to be required for P...”
- The P-Type ATPase PA1429 Regulates Quorum-Sensing Systems and Bacterial Virulence
Zhang, Frontiers in microbiology 2017 - “...Probable cation-transporting P-type ATPa 4 5450147 PA4854( purH ) Phosphoribosylaminoimidazolecarboxamide formyltransferase 2.5 Decreased expression 813117 PA0745 Probable enoyl-CoA hydratase/isomerase 2 2450231 PA2228 Hypothetical protein 10 1557569 PA3011 ( topA ) DNA topoisomerase I 3 1559485 PA1432 ( lasI ) Autoinducer synthesis protein LasI 8 1549342 PA1423...”
- “...a set of new loci that had not been described, such as PA1429, PA2228, and PA0745 ( Table 1 ). PA1429 Represses the Expression of pqsH Among the 11 transposon mutants, the PA1429 mutation resulted in significant increase in pqsH expression. PA1429 encodes a probable cation-transporting...”
- Proteomic Response of Pseudomonas aeruginosa PAO1 Adhering to Solid Surfaces
Guilbaud, Frontiers in microbiology 2017 - “...7.4 3.9E-03 6.7E-03 probable acyl-CoA dehydrogenase PA3972 2.0 2.2 5.8 3.8E-03 7.1E-03 probable enoyl-CoA hydratase/isomerase PA0745 2.4 5.6 8.0 1.8E-04 flavin-containing monooxygenase PA4217 phzS 1.2 3.0 5.0 8.9E-04 Hypothetical proteins hypothetical protein PA2330 1.6 1.0 6.2 3.5E-04 1.7E-05 conserved hypothetical protein PA1135 0.4 0.0 3.8 1.8E-04...”
- “...energy metabolism. In addition, 3 proteins belonging to the putative enzymes class, PA2815, PA3972, and PA0745, were less abundant in this condition and might be involved in lipid homeostasis (Table S5 ). Conversely, several proteins were more abundant in the context of SS/PS. This was the...”
- Mitochondrial targeting increases specific activity of a heterologous valine assimilation pathway in Saccharomyces cerevisiae
Solomon, Metabolic engineering communications 2016 - “...Westermann and Neupert, 2000 ). To simulate the metabolic burden of carboxylic acid production, echA3 (PA0745) and hchA (PA0744) encoding enoyl-CoA hydratases were cloned between the BglII and EcoRI sites of pYES-mtGFP with the primers in Table 2 to target expression to the mitochondria via the...”
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Atu1417 enoyl-CoA hydratase from Agrobacterium tumefaciens str. C58 (Cereon)
34% identity, 95% coverage
- Characterization of the first tetrameric transcription factor of the GntR superfamily with allosteric regulation from the bacterial pathogen Agrobacterium fabrum
Vigouroux, Nucleic acids research 2021 - “...is added to ferulic acid by Atu1416, a feruloyl-CoA synthase. Feruloyl-CoA is then converted by Atu1417, an enoyl-CoA hydratase, into 4-hydroxy-3-methoxyphenyl--hydroxypropionyl (HMPHP)-CoA, which is in turn transformed into vanillic acid by Atu1415, a phenylhydroxypropionyl-CoA dehydrogenase, and then Atu1421, a 4-hydroxy-3-methoxyphenyl--ketopropionyl-CoA (HMPKP)-CoA -keto-thiolase. The O-demethylase Atu1420 degrades...”
- “...degradation of ferulic acid leading to different intermediates ( 5 ): Atu1416 (a feruloyl-CoA synthase), Atu1417 (an enoyl-CoA hydratase), Atu1415 (a phenylhydroxypropionyl-CoA dehydrogenase), Atu1421 (a 4-hydroxy-3-methoxyphenyl--ketopropionyl-CoA -keto-thiolase) and Atu1420 (a tetrahydrofolate-dependent vanillate O-demethylase). Atu1420 shares 56% sequence identity with Sphingomonas paucimobilis and Sphingobium sp. SYK-6 homologues...”
- Identification of enzymatic genes with the potential to reduce biomass recalcitrance through lignin manipulation in Arabidopsis
Sakamoto, Biotechnology for biofuels 2020 - “...transgenes (Additional file 1 : Table S3). We failed to recover a transgenic plant carrying Atu1417 in the present study. The resulting transgenic plants were grown until inflorescence stems were fullyelongated. Hand-sliced cross sections of primary inflorescence stems from all 296 transgenic plants were stained with...”
- Functional Redundancy in the Hydroxycinnamate Catabolism Pathways of the Salt Marsh Bacterium Sagittula stellata E-37
Frank, Applied and environmental microbiology 2018 - “...Atu1415 (NP_354422) Atu1416 (NP_354423) Atu1416 (NP_354423) Atu1417 (NP_354425) Atu1421 (NP_354429) S. stellata homolog locus (NCBI RefSeq accession no.)...”
- Coordinated Regulation of Species-Specific Hydroxycinnamic Acid Degradation and Siderophore Biosynthesis Pathways in Agrobacterium fabrum
Baude, Applied and environmental microbiology 2016 - “...expression of genes involved in HCA degradation (atu1416, atu1417, and atu1420) Applied and Environmental Microbiology June 2016 Volume 82 Number 12 Downloaded...”
- “...(HMPHP)-CoA by the enoyl-CoA hydratase Atu1417. Two original enzymatic reactions then follow. HMPHPCoA is first converted...”
- Analysis of hydroxycinnamic acid degradation in Agrobacterium fabrum reveals a coenzyme A-dependent, beta-oxidative deacetylation pathway
Campillo, Applied and environmental microbiology 2014 - “...vanillic acid, and protocatechuic acid. The genes atu1416, atu1417, and atu1420 have been experimentally shown to be necessary for the degradation of ferulic...”
- “...described by Lassalle et al. (23). The atu1420 and atu1417 gene sequences were deleted and replaced by the Neo-Kan resistance gene nptII in the C58Atu1420 and...”
- Genomic species are ecological species as revealed by comparative genomics in Agrobacterium tumefaciens
Lassalle, Genome biology and evolution 2011 - “...functions that could be related to the same metabolic pathway, including: an enoyl-CoA hydratase (Ech) (Atu1417), a feruloyl-CoA dehydratase (Fcd) (Atu1414), a tetrahydrofolate-dependent vanillate O-demethylase (LigM) (Atu1420), and a methylenetetrahydrofolate reductase (MetF) (Atu1418), as well as substrate-binding regulators VanR (Atu1419) and FerR (Atu1422). Indeed, we were...”
AOC05_11215 enoyl-CoA hydratase from Arthrobacter alpinus
30% identity, 96% coverage
- Complete genome of Arthrobacter alpinus strain R3.8, bioremediation potential unraveled with genomic analysis
See-Too, Standards in genomic sciences 2017 - “...and 2-methylnaphthalene degradation, and other genes involved in 1,4-dichlorobenzene degradation, namely enoyl-CoA hydratase [AOC05_00980, AOC05_05365, AOC05_11215, AOC05_11270, AOC05_11290, AOC05_11310, AOC05_13700, AOC05_13710], alkaline phosphatase [AOC05_01425, AOC05_03310, AOC05_12520], nitrilotriacetate monooxygenase [AOC05_10370] and aliphatic amidase amiE [AOC05_16895]. Furthermore, two genes involved in the production of urease were also identified...”
AFUA_3G14520 enoyl-CoA hydratase/isomerase family protein from Aspergillus fumigatus Af293
Q4WYW7 Enoyl-CoA hydratase/isomerase family protein from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
29% identity, 91% coverage
PG1079 enoyl-CoA hydratase/isomerase family protein from Porphyromonas gingivalis W83
29% identity, 90% coverage
LOC103497788 probable enoyl-CoA hydratase 1, peroxisomal from Cucumis melo
33% identity, 74% coverage
- Study on the differences in aroma components and formation mechanisms of "Nasmi" melon from different production areas
Shao, Food science & nutrition 2022 - “...66.893 AcetylCoA carboxylase carboxyl transferase subunit alpha [EC:6.4.1.2 2.1.3.15] LOC103500505 111.556 553.813 AcetylCoA Cacetyltransferase [EC:2.3.1.9] LOC103497788 62.923 187.83 EnoylCoA hydratase [EC:4.2.1.17] LOC103501532 73.04 5.46 Fatty acylACP thioesterase B [EC:3.1.2.14 3.1.2.21] LOC103500074 174.696 2261.113 Alcohol dehydrogenase [EC:1.1.1.1] LOC103483849 1639.03 5491.8 Aldehyde dehydrogenase (NAD+) [EC:1.2.1.3] LOC103483275 26.67 41.816...”
AFT31_ALTAL / Q96VB3 Enoyl-CoA hydratase AFT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata) (see 3 papers)
28% identity, 82% coverage
- function: Enoyl-CoA hydratase; part of the gene clusters that mediate the biosynthesis of the host-selective toxins (HSTs) AF-toxins responsible for Alternaria black spot of strawberry disease by the strawberry pathotype (PubMed:12019223). AF-toxin I and III are valine derivatives of 2,3-dyhydroxy-isovaleric acid and 2-hydroxy-isovaleric acid respectively, while AF II is an isoleucine derivative of 2- hydroxy-valeric acid (PubMed:15066029, PubMed:22846083, Ref.2). These derivatives are bound to a 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (EDA) moiety (PubMed:15066029, PubMed:22846083, Ref.2). On cellular level, AF-toxins affect plasma membrane of susceptible cells and cause a sudden increase in loss of K(+) after a few minutes of toxin treatment (PubMed:22846083). The aldo-keto reductase AFTS1 catalyzes the conversion of 2-keto-isovaleric acid (2-KIV) to 2- hydroxy-isovaleric acid (2-HIV) by reduction of its ketone to an alcohol (PubMed:15066029). The acyl-CoA ligase AFT1, the hydrolase AFT2 and the enoyl-CoA hydratases AFT3 and AFT6, but also the polyketide synthase AFT9, the acyl-CoA dehydrogenase AFT10, the cytochrome P450 monooxygenase AFT11 and the oxidoreductase AFT12 are all involved in the biosynthesis of the AK-, AF- and ACT-toxin common EDA structural moiety (PubMed:12019223, PubMed:18986255, Ref.2). The exact function of each enzyme, and of additional enzymes identified within the AF-toxin clusters have still to be determined (PubMed:12019223, PubMed:18986255, Ref.2).
catalytic activity: a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (RHEA:16105)
catalytic activity: a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (RHEA:20724)
disruption phenotype: Abolishes the production of AF-toxins and their precuror 9,10-epoxy-8-hydroxy-9-methyl-decatrienoic acid (EDA); and impairs the pathogenicity (PubMed:12019223). Does not affect growth rate of cultures, sporulation, and spore germination (PubMed:12019223).
O35459 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial from Mus musculus
NP_058052 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor from Mus musculus
26% identity, 79% coverage
- Adenosine-rich extract of Ganoderma lucidum: A safe and effective lipid-lowering substance.
Li, iScience 2022 - “...() P55096 Abcd3 down () up: 260 () Q9DBM2 Ehhadh down () down: 240 () O35459 Ech1 down () up: 316 () Q921G7 Etfdh down () up: 222 () Q99LC5 Etfa down () up: 206 () Q9R0H0 Acox1 down () up: 260 () up: 260 ()...”
- Effects of microgravity exposure and fructo-oligosaccharide ingestion on the proteome of soleus and extensor digitorum longus muscles in developing mice.
Ohira, NPJ microgravity 2021 - “...P =3.3E1 Etfdh (Q921G7) * FC=0.70, P =2.2E3 FC=0.80, P =1.1E2 FC=0.96, P =5.8E1 Ech1 (O35459) * FC=0.38, P =6.1E4 * FC=0.46, P =1.1E3 FC=0.92, P =3.7E1 Eci1 (P42125) * FC=0.64, P =1.8E3 * FC=0.72, P =1.1E3 FC=0.93, P =3.4E1 Eci2 (Q9WUR2) * FC=0.71, P =1.5E3...”
- Functional, proteomic and bioinformatic analyses of Nrf2- and Keap1- null skeletal muscle
Gao, The Journal of physiology 2020 - “...Hadha Q8BMS1 Trifunctional enzyme subunit alpha, mitochondrial 0.04 1.71 Ttn A2ASS6 Titin 0.04 1.25 Ech1 O35459 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 0.04 1.59 Hspb7 P35385 Heat shock protein beta-7 0.04 1.56 Slc27a1 Q60714 Long-chain fatty acid transport protein 1 0.04 1.57 Afg3l2 Q8JZQ2 AFG3-like protein 2 0.04 1.84...”
- PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.
Feng, International journal of oncology 2020 - “...Paired box protein Pax-2 2.83 Up 0.024 Q8BWT1 Acaa2 3-ketoacyl-CoA thiolase, mitochondrial 2.06 Up 0.0101 O35459 Ech1 Delta( 3 , 5 )-Delta( 2 , 4 )-dienoyl-CoA isomerase, mitochondrial 1.74 Up 0.0036 Q9DBM2 Ehhadh Peroxisomal bifunctional enzyme 1.71 Up 0.0032 Q61425 Hadh Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial 1.69...”
- Mice depleted for Exchange Proteins Directly Activated by cAMP (Epac) exhibit irregular liver regeneration in response to partial hepatectomy.
Sivertsen, Scientific reports 2019 - “...O35728 Cyp4a14 Cytochrome P450 4A14 9.89 13.73 Q9CQ62 Decr1 2,4-dienoyl-CoA reductase, mitochondrial 1.19, ns 1.31 O35459 Ech1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 1.31 1.35 Q9DBM2 Ehhadh Peroxisomal bifunctional enzyme; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase 1.18, ns 1.59 P51660 Hsd17b4 Peroxisomal multifunctional enzyme type 2;(3R)-hydroxyacyl-CoA dehydrogenase; Enoyl-CoA hydratase 2...”
- Proteomic and transcriptomic study of brain microvessels in neonatal and adult mice.
Porte, PloS one 2017 - “...3.89 - - - P54869 Hmgcs2 1.740.12 3.520.51 1.810.29 0.10 5.23 - - 8 - O35459 Ech1 2.540.43 5.431.07 5.520.34 4.161.66 e 18.603 9.503.70 - 33 - P47738 Aldh2 2.460.13 4.490.41 10.250.54 2.212.17 3.991.75 18.264.46 - 37 49 P05064 Aldoa 52.0816.82 52.085.97 104.6512.28 92.35 113.05 906.50...”
- Multifactorial comparative proteomic study of cytochrome P450 2E1 function in chronic alcohol administration.
Wang, PloS one 2014 - “...Peroxiredoxin 6 Prdx6 * oxidation reduction - 2.3 Q5M9M5 MCG10806 Rpl23a DNA binding - 2.62 O35459 Betainehomocysteine S-methyltransferase 1 Bhmt * amino acid metabolism - 2.74 Q3UIA9 Fumarate hydratase 1 Fh1 fatty acid metabolism - 2.87 Q63880 Liver carboxylesterase 31 Es31 alcohol metabolism - 3.19 Q56A15...”
- Comparative differential proteomic profiles of nonfailing and failing hearts after in vivo thoracic aortic constriction in mice overexpressing FKBP12.6
Prévilon, Physiological reports 2013 - “...protein 1 O70400 6.5 36 6.4 36 2 74 38 7 58 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial O35459 6.3 31 7.6 36 7 404 92 25 60 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial O35459 6.0 31 7.6 36 2 95 61 8 62 Malate dehydrogenase, cytoplasmic P14152 5.9 34 6.2 36...”
- More
- Enoyl coenzyme A hydratase 1 alleviates nonalcoholic steatohepatitis in mice by suppressing hepatic ferroptosis.
Liu, American journal of physiology. Endocrinology and metabolism 2021 (PubMed)- GeneRIF: Enoyl coenzyme A hydratase 1 alleviates nonalcoholic steatohepatitis in mice by suppressing hepatic ferroptosis.
- Enoyl coenzyme A hydratase 1 combats obesity and related metabolic disorders by promoting adipose tissue browning.
Mao, American journal of physiology. Endocrinology and metabolism 2020 (PubMed)- GeneRIF: ECH1 regulated the thermogenic program by inhibiting mammalian target of rapamycin signaling, which may partially explain the potential mechanism for ECH1 regulating adipose browning
- Enoyl coenzyme A hydratase 1 protects against high-fat-diet-induced hepatic steatosis and insulin resistance.
Huang, Biochemical and biophysical research communications 2018 (PubMed)- GeneRIF: Ech1 protects against high-fat-diet-induced hepatic steatosis and insulin resistance.
- Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma.
Zhang, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2013 (PubMed)- GeneRIF: current work indicates the involvement of Ech1 in tumor metastasis development and progression, but the subcellular location of Ech1 has not much contribution to that.
- [Down-regulation of Ech1 decreases the adhesion ability of mouse hepatocarcinoma Hca-F cells].
Zhang, Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology 2012 (PubMed)- GeneRIF: Down-regulation of Ech1 can inhibit the adhesive capacity of metastatic Hca-F cells.
- Enoyl coenzyme A hydratase 1 is an important factor in the lymphatic metastasis of tumors.
Zhang, Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2011 (PubMed)- GeneRIF: The downregulation of Ech1 inhibited proliferation of the Hca-F cells, increased the ratio of Hca-F cells in S phase to G(1) phase and decreased the adhesion and migration capacities of Hca-F cells.
- [Expression of enoyl CoA hydratase 1 reduces cell proliferation and migration in mouse hepatocarcinoma cells].
Zhang, Zhonghua bing li xue za zhi = Chinese journal of pathology 2011 (PubMed)- GeneRIF: Down-regulation of ECH1 could inhibit the cell proliferation and migration of Hca-F hepatocarcinoma cells.
- Overexpression of peroxisome proliferator-activated receptor-alpha (PPARalpha)-regulated genes in liver in the absence of peroxisome proliferation in mice deficient in both L- and D-forms of enoyl-CoA hydratase/dehydrogenase enzymes of peroxisomal beta-oxidation system.
Jia, The Journal of biological chemistry 2003 (PubMed)- GeneRIF: disruption of peroxisomal fatty acid beta-oxidation at the level of enoyl-CoA hydratase/L-3-hydroxyacyl-CoA dehydrogenase (L-PBE) in mice leads to the induction of many of the PPARalpha target genes independently of peroxisome proliferation in hepatocytes
POX_g08550 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase from Penicillium oxalicum
30% identity, 86% coverage
- Regulation of fungal raw-starch-degrading enzyme production depends on transcription factor phosphorylation and recruitment of the Mediator complex
Ning, Communications biology 2023 - “...ANOVA. RSDE raw starch degrading enzyme, SSDE soluble starch degrading enzyme. PoxRsrA interacts with protein POX_g08550 Tandem affinity purification-mass spectroscopy (TAP-MS) was applied to detect proteins that may interact with PoxRsrA. Recombinant protein His-PoxRsrA 9011360 -GFP was produced by E. coli and used as a probe...”
- “...confirmation of the above interactions. Only the experiments confirming interaction between PoxRsrA 9011360 and candidate POX_g08550 are shown; the 11 other candidates require further study. For the GST-pulldown assay, the fusion proteins PoxRsrA 9011360 -GST and POX_g08550-His were produced by E . coli , and purified....”
PNUC_RS08130 enoyl-CoA hydratase from Polynucleobacter asymbioticus QLW-P1DMWA-1
29% identity, 95% coverage
F4JML5 methylglutaconyl-CoA hydratase (EC 4.2.1.18) from Arabidopsis thaliana (see paper)
26% identity, 81% coverage
- Identification of the Candidate Proteins Related to Oleic Acid Accumulation during Peanut (Arachis hypogaea L.) Seed Development through Comparative Proteome Analysis
Liu, International journal of molecular sciences 2018 - “...1.61 Q9SW21 ACP4 fatty acid biosynthetic process Araip.10019224.1 1.47 O82399 PMDH1 lipid oxidation Araip.10019122.1 1.46 F4JML5 At4g16800 lipid oxidation Araip.10033415.1 1.44 Q9ZP05 PMDH2 regulation of lipid catabolic process Araip.10019397.1 1.27 Q9LD43 CAC3 fatty acid biosynthetic process Araip.10008554.1 1.27 Q9M8L4 GLPK lipid oxidation Araip.10034912.1 1.27 Q9FLH8 At5g51830...”
- “...P33207 At1g24360 fatty acid biosynthetic process Araip.10037915.1 1.67 Q9SQI8 LTA2 lipid biosynthetic process Araip.10019122.1 1.64 F4JML5 At4g16800 fatty acid metabolic process Araip.10020945.1 1.62 Q9SW21 ACP4 fatty acid biosynthetic process Araip.10014644.1 1.62 Q9SS98 At3g01570 lipid storage Araip.10014038.1 1.34 Q9M7Z1 BCE2 fatty acid biosynthetic process Araip.10039882.1 1.33 Q8L9C4...”
AR1Y2_1116 enoyl-CoA hydratase-related protein from Anaerostipes rhamnosivorans
28% identity, 94% coverage
- Conversion of dietary inositol into propionate and acetate by commensal Anaerostipes associates with host health
Bui, Nature communications 2021 - “...including a 3-oxoacid CoA transferase (OxcT encoded by AR1Y2_1115), an enoyl-CoA dehydratase (AcaD encoded by AR1Y2_1116) and an acyl dehydrogenase (EcdH encoded by AR1Y2_1117) and acyl dehydrogenase complex (AcaD-Etf encoded by AR1Y2_1050-1052). CO 2 /H 2 or formate is also formed from a conversion of pyruvate...”
- “...(OxcT encoded by AR1Y2_1115), acyl-CoA dehydrogenase (AcaD encoded by AR1Y2_1117), enoyl-CoA hydratase (EcdH encoded by AR1Y2_1116) and acryloyl-CoA dehydrogenase/Etf (AcaD-Etf encoded by AR1Y2_1050-1052 ) . Interestingly, oxcT, ecdH and acaD (AR1Y2_1115-1117) were located in a putative operon. The 3-oxoacid CoA transferase protein (OxcT encoded by AR1Y2_1115)...”
BA2551 enoyl-CoA hydratase/isomerase family protein from Bacillus anthracis str. Ames
29% identity, 93% coverage
PGN_1175 putative enoyl-CoA hydratase from Porphyromonas gingivalis ATCC 33277
29% identity, 90% coverage
ferB2 / Q8RR26 feruloyl-CoA hydratase/lyase from Sphingomonas paucimobilis (see paper)
ferB2 / BAB86296.1 feruloyl-CoA hydratase/lyase from Sphingomonas paucimobilis (see paper)
29% identity, 83% coverage
SGRA_p0039 enoyl-CoA hydratase-related protein from Saprospira grandis str. Lewin
26% identity, 95% coverage
YP_482743 Enoyl-CoA hydratase/isomerase from Frankia sp. CcI3
35% identity, 73% coverage
- Distribution analysis of hydrogenases in surface waters of marine and freshwater environments
Barz, PloS one 2010 - “...Chromobacterium violaceum ATCC 12472 NP_903812; Daroma, Dechloromonas aromatica RCB YP_287160; Frankia Cc Frankia sp. CcI3 YP_482743; Frankia EA Frankia sp. EAN1pec YP_001505433; MmagAMB, Magnetospirillum magneticum AMB-1 YP_420998; MmagMS-1, Magnetospirillum magnetotacticum MS-1 ZP_00055441; Mmarina Microscilla marina ATCC 23134 ZP_01691397; Mpetro, Methylibium petroleiphilum PM1 YP_001021998; Ncaesar, Neptuniibacter caesariensis...”
Tneu_0541 3-hydroxyacyl-CoA dehydrogenase NAD-binding from Thermoproteus neutrophilus V24Sta
Tneu_0541 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein from Pyrobaculum neutrophilum V24Sta
30% identity, 37% coverage
- Identification of missing genes and enzymes for autotrophic carbon fixation in crenarchaeota
Ramos-Vera, Journal of bacteriology 2011 - “...Igni_0595); 14, crotonyl-CoA hydratase (Msed_0399, Tneu_0541, Igni _1058); 15, (S)-3-hydroxybutyryl-CoA dehydrogenase (NAD) (Msed_0399, Tneu_0541, Igni _1058);...”
- “...dehydrogenase (N-terminal domain, 40 kDa) (Msed_0399, Tneu_0541, Igni_1058). Autotrophic Sulfolobales contained additional, less similar candidate genes coding...”
- The complete genome sequence of Thermoproteus tenax: a physiologically versatile member of the Crenarchaeota
Siebers, PloS one 2011 - “...Tneu_0420 (82%, 0.0) 4-Hydroxybutyryl-CoA dehydratase TTX_1102 Tneu_0422 (100%, 0.0) Crotonyl-CoA hydratase/( S )-3-Hydroxybutyryl-CoA DH TTX_1028 Tneu_0541 (99%, 0.0) Igni_1058 (99%, 1e-160) Acetoacetyl-CoA -ketothiolase TTX_0886 Tneu_0249 (99%, 1e-166) Igni_1401 (99%, 2e-104) The corresponding e-values derived from blastp analyses of the T. neutrophilus (Tneu_) and I. hospitalis (Igni_)...”
- Regulation of autotrophic CO2 fixation in the archaeon Thermoproteus neutrophilus
Ramos-Vera, Journal of bacteriology 2010 - “...Tneu_1204 Tneu_0418 Tneu_1509 Tneu_1464 Tneu_1463 Tneu_0420 Tneu_0422 Tneu_0541 Enzymea VOL. 192, 2010 REGULATION OF AUTOTROPHY IN THERMOPROTEUS 5335 scription...”
- (S)-3-Hydroxybutyryl-CoA Dehydrogenase From the Autotrophic 3-Hydroxypropionate/4-Hydroxybutyrate Cycle in Nitrosopumilus maritimus
Liu, Frontiers in microbiology 2021 - “...are marked as following: purple triangle, Nmar_1028; red triangle, Msed_1423; red star, Msed_0399; olive star, Tneu_0541 (from Pyrobaculum neutrophilum ); turquoise star, Igni_1058 (from Ignicoccus hospitalis ); , dehydrogenase; without , fusion protein; 80 sequences from TACK group in red, 15 sequences from non-AOA Thaumarchaeota in...”
UW3_RS0120745 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase PaaG from Pseudomonas donghuensis
30% identity, 92% coverage
- The Gene paaZ of the Phenylacetic Acid (PAA) Catabolic Pathway Branching Point and ech outside the PAA Catabolon Gene Cluster Are Synergistically Involved in the Biosynthesis of the Iron Scavenger 7-Hydroxytropolone in Pseudomonas donghuensis HYS
Wang, International journal of molecular sciences 2023 - “...), ech , and NodN from P. donghuensis HYS are UW3_RS0120700, UW3_RS0120705, UW3_RS0120710, UW3_RS0120715, UW3_RS0120720, UW3_RS0120745, UW3_RS0120680, UW3_RS0113785, and UW3_RS0112810, respectively. 5. Conclusions In summary, this study is the first to report that the paaABCDE and paaG genes in cluster 2 are involved in the biosynthesis...”
S5LPF1 Enoyl-CoA hydratase/aldolase (Fragment) from Streptomyces sp. V-1
30% identity, 73% coverage
- Biosynthesis of Vanillin by Rational Design of Enoyl-CoA Hydratase/Lyase
Ye, International journal of molecular sciences 2023 - “...of Ech The targeted Ech sequence was selected from Streptomyces sp. strain V-1 (accession number: S5LPF1) in UniProt database. The multiple sequence alignment (MSA) of Ech were performed by retrieving 250 sequences which exhibit more than 60% identity with the targeted sequence from UniProt database. The...”
- “...synthase (Fcs, accession number: S5M744), and the other is the enoyl-CoA hydratase/lyase (Ech, accession number: S5LPF1). Sequence alignments were performed towards Ech, sequences with more than 60 percent homology were retrieved, and the ClusterW [ 32 ] algorithm was used to calculate the multiple sequence alignment....”
- Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization
Moraes, Biotechnology for biofuels 2018 - “...acid identity to Pseudomonas nitroreducens (C3VA24), followed by Amycolatopsis methanolica (59%; A0A076MUC3), Streptomyces sp. (55%; S5LPF1), and D. acidovorans (55%; Q8VNW7). Amino acid sequences from NCBI database and Uniprot with similarity higher than 20% to FerA_B3 and FerB_B11 were considered to construct phylogenetic trees (Additional file...”
H16_B1738 Enoyl-CoA hydratase/isomerase family from Ralstonia eutropha H16
31% identity, 95% coverage
Q13011 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial from Homo sapiens
NP_001389 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial precursor from Homo sapiens
27% identity, 76% coverage
- Combined High-Throughput Proteomics and Random Forest Machine-Learning Approach Differentiates and Classifies Metabolic, Immune, Signaling and ECM Intra-Tumor Heterogeneity of Colorectal Cancer
Contini, Cells 2024 - “...[ 47 ] P10606 ( COX5B ) Cytochrome c oxidase sub. 5B 0.0012 D<H 4.5 Q13011 ( ECH1 ) DI (mitochondrial) 0.0007 D<H 3.3 P36957 ( DLST ) DLST 0.0008 D<H 3.4 0.0011 O95571 ( ETHE1 ) ETHE1 0.0000 D<H 4.0 0.0002 0.02 IHC, WB, LCMS/MS...”
- ProtGraph: a tool for the quick and comprehensive exploration and exploitation of the peptide search space derived from protein sequence databases using graphs.
Lux, Briefings in bioinformatics 2024 - “...(P68871) VAR_003077 6 106 SORD (Q00796) VAR_060351 236 HBA (P69905) VAR_002783 VAR_002789 7 86 ECH1 (Q13011) VAR_014927 235 HBA (P69905) VAR_002793 VAR_012662 8 82 HBA (P69905) VAR_002799 VAR_002808 VAR_002810 198 HBA (P69905) VAR_066401 9 82 ADH1C (P00326) VAR_000429 181 HBB (P68871) VAR_002923 VAR_002926 VAR_002927 10 77...”
- Generation and Characterization of Trastuzumab/Pertuzumab-Resistant HER2-Positive Breast Cancer Cell Lines.
Sanz-Álvarez, International journal of molecular sciences 2023 - “...FMR1 2.72 1.45 1 11 173 Q9Y3B8 REXO2 Oligoribonuclease, mitochondrial 2.66 1.41 1 8 95 Q13011 ECH1 Delta(3,5)-delta(2,4)-dienoyl-CoA isomerase, mitochondrial 2.62 1.39 8 79 1337 Q8NEJ9 NGDN Neuroguidin 2.60 1.38 3 11 189 P23434 GCSH Glycine cleavage system H protein, mitochondrial 2.57 1.36 2 7 129...”
- Effects of Physiological and Pathological Urea Concentrations on Human Microvascular Endothelial Cells
Colombo, International journal of molecular sciences 2022 - “...12.660 0.000001995520621 P29401 Transketolase 11.977 0.000003566403024 Q16658 Fascin 11.654 0.000004723432374 Q9BTY2 Plasma alpha-L-fucosidase 11.477 0.000018168658036 Q13011 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial 11.337 0.000007886470783 PFAS Phosphoribosylformylglycinamidine synthase, isoform CRA_b 11.234 0.000006831656478 Q53T99 Ribosome biogenesis protein WDR12 9.173 0.000048900585780 Q6EMK4 Vasorin 9.026 0.000641197347120 V9HWF4 Phosphoglycerate kinase 8.109 0.000151428360979 G3V180 Dipeptidyl...”
- The muscle proteome reflects changes in mitochondrial function, cellular stress and proteolysis after 14 days of unilateral lower limb immobilization in active young men
Doering, PloS one 2022 - “...P28289 TMOD1 Tropomodulin-1 1.35 1.50E-03 Negatively enriched proteins Q92523 CPT1B Carnitine O-palmitoyltransferase 1 -1.35 3.80E-03 Q13011 ECH1 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial -1.32 6.87E-03 Q9H511 KLH31 Kelch-like protein 31 -2.14 7.09E-03 P52815 RM12 39S ribosomal protein L12, mitochondrial -1.31 1.41E-02 P35613 BASI Basigin -1.29 1.42E-02 P45880 VDAC2 Voltage-dependent...”
- Alterations of actin cytoskeleton and arterial protein level in patients with obstructive jaundice.
Wang, Genetics and molecular biology 2022 - “...nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. LMNA Intermediate filament, nucleus. Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Q13011 -1.45960 Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. ECH1 Mitochondrion, peroxisome. Galactokinase P51570 1.86176 Catalyzes the transfer of a phosphate from ATP to alpha-D-galactose and participates in the first committed step in...”
- Proteome profiling of human placenta reveals developmental stage-dependent alterations in protein signature
Khorami, Clinical proteomics 2021 - “...2.173 Cellular catabolic process 4.259E-04 7 P63261 ACTG 0.372 0.7053 0.527 Blood coagulation 5.944E-03 8 Q13011 ECH1 0.169 0.0976 1.731 Metabolic pathways 2.595E-03 9 Q13162 PRDX4 0.2158 0.0788 2.738 Response to stress 6.958E-04 10 P30040 ERP29 0.1616 0.042 3.847 Protein folding 7.616E-05 11 P30084 ECHM 0.1258...”
- Acquisition of Letrozole Resistance Through Activation of the p38/MAPK Signaling Cascade
Walker, Anticancer research 2021 - “...of cell death OS=Homo sapiens GN=BAD PE=1 SV=1 - [F5GYS3_HUMAN] 157 16.6392 1.5392774 p <0.01 Q13011 Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens GN=ECH1 PE=1 SV=2 - [ECH1 _HUMAN] 328 35.7934 1.5385737 p <0.01 P05091 Aldehyde dehydrogenase, mitochondrial OS=Homo sapiens GN=ALDH2 PE=1 SV=2 - [ALDH2_HUMAN] 517 56.3456 1.5385587...”
- More
- Enoyl coenzyme A hydratase 1 alleviates nonalcoholic steatohepatitis in mice by suppressing hepatic ferroptosis.
Liu, American journal of physiology. Endocrinology and metabolism 2021 (PubMed)- GeneRIF: Enoyl coenzyme A hydratase 1 alleviates nonalcoholic steatohepatitis in mice by suppressing hepatic ferroptosis.
- Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson, PloS one 2010 - GeneRIF: Observational study of gene-disease association. (HuGE Navigator)
- Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue.
Kovalyov, Biochemistry. Biokhimiia 2006 (PubMed)- GeneRIF: Polymorphism of delta3,5-delta2,4-dienoyl-coenzyme A isomerase (the ECH1 gene product protein) in human striated muscle tissue
- Isolation and characterization of rat and human cDNAs encoding a novel putative peroxisomal enoyl-CoA hydratase.
FitzPatrick, Genomics 1995 (PubMed)- GeneRIF: Possible roles for the ECH1 protein product in peroxisomal beta-oxidation are discussed.
PADG_05773 uncharacterized protein from Paracoccidioides brasiliensis Pb18
29% identity, 68% coverage
- Prediction of Conserved Peptides of Paracoccidioides for Interferon-γ Release Assay: The First Step in the Development of a Lab-Based Approach for Immunological Assessment during Antifungal Therapy
Rosa, Journal of fungi (Basel, Switzerland) 2020 - “...Carrier ADP/ATP PAAG_08620 0.0 100% 97% Carrier calcium PAAG_07762 0.0 100% 98% Carnitil CoA dehydrogenase PADG_05773 0.0 100% 97% Catalase PAAG_01454 0.0 100% 98% Catalase PADG_00324 0.0 98% 100% Catalase PAAG_01943 0.0 99% 99% Chitin synthase VII class ABV31248.1 0.0 98% 99% 1,3 ketoacyl-CoA lyase PADG_07365...”
- Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection
Lacerda, Virulence 2017 - “...pathway, which includes acyl-CoA dehydrogenases (PADG_07604, PADG_05046, PADG_06335), short chain dehydrogenases/reductases (PADG_06247, PADG_06006), carnitine transport/metabolism (PADG_05773, PADG_06378), peroxisomal hydratase-dehydrogenase-epimerase (PADG_08651) and a 3-hydroxybutyryl-CoA dehydratase (PADG_05039). These genes demonstrated increase transcript abundance that ranged from 2.29 to 17.00 (TableS1). In addition, ethanol producing genes were induced including...”
- “...number a Gene Description b Ratio Infection/Control c Function d PADG_06490 Formamidase 22.77 Nitrogen metabolism PADG_05773 Carnitinyl-CoA dehydratase 17.00 Lipid, fatty acid and isoprenoid metabolism PADG_07604 Acyl-CoA dehydrogenase 13.46 Fatty acid degradation PADG_08544 GABA permease 10.80 Transport PADG_07751 Mitochondrial integral membrane protein 8.35 Transport PADG_05876 Centromere/microtubule-binding...”
H16_B0419 Enoyl-CoA hydratase from Ralstonia eutropha H16
31% identity, 72% coverage
FGSG_00704 hypothetical protein from Fusarium graminearum PH-1
28% identity, 81% coverage
Nmar_1308 Enoyl-CoA hydratase/isomerase from Nitrosopumilus maritimus SCM1
27% identity, 83% coverage
- Structural insights into bifunctional thaumarchaeal crotonyl-CoA hydratase and 3-hydroxypropionyl-CoA dehydratase from Nitrosopumilus maritimus
Destan, Scientific reports 2021 - “...of the most energy-efficient CO 2 fixation cycles discovered thus far. The protein encoded by Nmar_1308 (from Nitrosopumilus maritimus SCM1) is a promiscuous enzyme that catalyzes two essential reactions within the thaumarchaeal 3HP/4HB cycle, functioning as both a crotonyl-CoA hydratase (CCAH) and 3-hydroxypropionyl-CoA dehydratase (3HPD). In...”
- “...fewer than the Calvin-Benson-Bassham cycle 1 . Figure 1 The dual enzymatic steps catalyzed by Nmar_1308 in the thaumarcheal 3HP/4HB cycle. Representation of 3HP/4HB cycle reactions in the thaumarcheal variant. The reaction showing the energy efficiency of the taumarcheal cycle is colored in red. Highlighted rectangle...”
- Structural Insights into Bifunctional Thaumarchaeal Crotonyl-CoA Hydratase and 3-Hydroxypropionyl-CoA Dehydratase from Nitrosopumilus maritimus
Destan, 2021 - Convergent Evolution of a Promiscuous 3-Hydroxypropionyl-CoA Dehydratase/Crotonyl-CoA Hydratase in Crenarchaeota and Thaumarchaeota
Liu, mSphere 2021 - “...aforementioned archaeal groups by a promiscuous 3-hydroxypropionyl-CoA dehydratase/crotonyl-CoA hydratase (Msed_2001 in crenarchaeon Metallosphaera sedula and Nmar_1308 in thaumarchaeon Nitrosopumilus maritimus ). Although these two enzymes are homologous, they are closely related to bacterial enoyl-CoA hydratases and were retrieved independently from the same enzyme pool by the...”
- “...grown M. sedula , at best. TABLE4 Catalytic properties of 3-hydroxypropionyl-CoA dehydrases/crotonyl-CoA hydratases Msed_2001 and Nmar_1308 as well as homologous enoyl-CoA hydratase Slip_2089 from S. lipocalidus d Substrate Msed_2001 Nmar_1308 Slip_2089 V max (Umg 1 protein) K m (mM) k cat / K m (s 1...”
- Stress response of a marine ammonia-oxidizing archaeon informs physiological status of environmental populations
Qin, The ISME journal 2018 - “...synthetase (Nmar_1309), 3-hydroxybutyryl-CoA dehydratase (Nmar_1308), 4-hydroxybutyryl-CoA dehydratase (Nmar_0207), and acetoacetyl-CoA -ketothiolase...”
- Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO2 fixation
Könneke, Proceedings of the National Academy of Sciences of the United States of America 2014 - “...160 20 1.5 Nmar_0272/0273/0274 ? ? Nmar_1309 Nmar_1308 ? Nmar_0272/0273/0274 Nmar_0953 Nmar_0954/0958 ? ? Nmar_0206 Nmar_0207 Nmar_1308 Nmar_1028 Nmar_0841...”
- “...an enoyl-CoA hydratase of the crotonase family (Nmar_1308) that may represent a 3-hydroxypropionyl-CoA dehydratase, the next enzyme in the reaction sequence....”
- A metaproteomic assessment of winter and summer bacterioplankton from Antarctic Peninsula coastal surface waters
Williams, The ISME journal 2012 - “...dehydratase Nmar_0207 Crotonyl-CoA hydratase Nmar_1308 3-Hydroxybutyryl-CoA dehydrogenase Nmar_1028 (NAD ) Acetoacetyl-CoA beta-ketothiolase Nmar_1631...”
- 3-hydroxypropionyl-coenzyme A dehydratase and acryloyl-coenzyme A reductase, enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in the Sulfolobales
Teufel, Journal of bacteriology 2009 - “...autotrophic marine group I Archaea (Nitrosopumilus sp. Nmar_1308 and Cenarchaeum sp. CENSYa_0166) shows only moderate similarity with the Metallosphaera en-...”
- “...acid sequence identity and 65 to 69% similarity for Nmar_1308 and 65% CENSYa_0166). It should be stressed, however, that the enoyl-CoA hydratase family and the...”
CBG09979 Protein CBG09979 from Caenorhabditis briggsae
28% identity, 70% coverage
WP_004885783 enoyl-CoA hydratase-related protein from Acinetobacter nosocomialis
27% identity, 93% coverage
F1NSS6 Enoyl-CoA hydratase domain containing 2 from Gallus gallus
33% identity, 57% coverage
BAU10_20670 enoyl-CoA hydratase-related protein from Vibrio alginolyticus
29% identity, 86% coverage
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory