PaperBLAST
PaperBLAST Hits for sp|Q9I0M7|CYSG_PSEAE Siroheme synthase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=cysG PE=3 SV=1 (465 a.a., MDFLPLFHSL...)
Show query sequence
>sp|Q9I0M7|CYSG_PSEAE Siroheme synthase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=cysG PE=3 SV=1
MDFLPLFHSLQGRLALVVGGGEVALRKARLLADAGARLRVVAPQIHIELRHLVEQGGGEL
LERDYQDGDQPGCALIIAATDDEPLNAEVSRAANARGIPVNVVDAPALCSVIFPAIVDRS
PLVVAVSSGGDAPVLARLIRAKLETWIPSTYGQLAGLASRFRHRVKELLPDLQQRRVFWE
NLFQGEIAERVLAGRPAEAERLLEEHLAGGLAHIATGEVYLVGAGPGDPDLLTFRALRLM
QQADVVLYDRLVAPSILELCRRDAERLYVGKRRAEHAVPQDRINRLLVELASQGKRVLRL
KGGDPFIFGRGGEEIDELAAHGIPFQVVPGITAASGCAAYAGIPLTHRDHAQSVRFVTGH
LKDGTTDLPWQDLVAPGQTLVFYMGLVGLPVICEQLVAHGRSAQTPAALIQQGTTAQQRV
FTGTLENLPQLVAEHEVHAPTLVIVGEVVQLRDKLAWFEGAREDA
Running BLASTp...
Found 252 similar proteins in the literature:
PA2611 siroheme synthase from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
W6QWV1 Siroheme synthase from Ectopseudomonas oleovorans (strain CECT 5344)
79% identity, 100% coverage
PP_3999 uroporphyrin-III C-methyltransferase from Pseudomonas putida KT2440
78% identity, 100% coverage
ABUW_0853 siroheme synthase CysG from Acinetobacter baumannii
57% identity, 98% coverage
D0CDR1 Siroheme synthase from Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
57% identity, 98% coverage
AHA_4121 siroheme synthase from Aeromonas hydrophila subsp. hydrophila ATCC 7966
55% identity, 98% coverage
STY4319 siroheme synthase from Salmonella enterica subsp. enterica serovar Typhi str. CT18
54% identity, 99% coverage
CysG / b3368 siroheme synthase (EC 2.1.1.107; EC 1.3.1.76; EC 4.99.1.4) from Escherichia coli K-12 substr. MG1655 (see 3 papers)
cysG / P0AEA8 siroheme synthase (EC 2.1.1.107; EC 1.3.1.76; EC 4.99.1.4) from Escherichia coli (strain K12) (see 10 papers)
CYSG_ECOLI / P0AEA8 Siroheme synthase; EC 2.1.1.107; EC 1.3.1.76; EC 4.99.1.4 from Escherichia coli (strain K12) (see 5 papers)
cysG / RF|NP_417827 siroheme synthase; EC 1.3.1.76; EC 2.1.1.107; EC 4.99.1.4 from Escherichia coli K12 (see 8 papers)
b3368 fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase from Escherichia coli str. K-12 substr. MG1655
NP_417827 siroheme synthase from Escherichia coli str. K-12 substr. MG1655
Z4729 uroporphyrinogen III methylase; sirohaeme biosynthesis from Escherichia coli O157:H7 EDL933
NJ74_RS16775 siroheme synthase CysG from Escherichia coli DH5[alpha]
54% identity, 99% coverage
- function: Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
catalytic activity: precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+) (RHEA:15613)
catalytic activity: siroheme + 2 H(+) = sirohydrochlorin + Fe(2+) (RHEA:24360)
subunit: Monomer. - Genome-scale identification and characterization of moonlighting proteins
Khan, Biology direct 2014 - “...For E. coli examples of two-domain proteins listed in Table 1 include ThrA (b0002) CysG (b3368) MetL (b3940) NadR (b4390) HldE (b3052) SpoT (b3650) to name just a few. In addition ATF2 PutA neuropilin-I and BirA which are discussed on pp. 23-24 and displayed on Figure...”
- “...These proteins include all the six proteins pointed out by the reviewer, ThrA (b0002), CysG (b3368), MetL (b3940), NadR (b4390), HldE (b3052), and SpoT (b3650) and four more proteins. (NadR was found in the MoonProt database, too). We excluded these ten multi-domain proteins from Table 1...”
- Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...(b0785) bmocogdp[c] moeA (b0826) bmocogdp[c] moeB (b0827) bmocogdp[c] ubiX (b2311) 2ohph[c] iscS (b2530) bmocogdp[c] cysG (b3368) sheme[c] mobA (b3857) bmocogdp[c] ubiC (b4039) 2ohph[c] Two false positive cases could be explained by incorrect gene-protein-reaction associations (GPRs) in i JO1366 (Table 6 ). In one, the gene hisH...”
- “...one condition, possible error argG (b3172) only experimental growth under one condition, possible error cysG (b3368) only experimental growth under one condition, possible error bioH (b3412) possibly biotin in media due to incomplete washing ilvE (b3770) only experimental growth under one condition, possible error thiH (b3990)...”
- Profiling of methyltransferases and other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Lenz, Journal of visualized experiments : JoVE 2010 - “...2-MTase small molecule 2 0 0 0 3 CysG b3368 50.0 Siroheme synthase includes uroporphyrinogen-III C-MTase small molecule 1 0 0 0 2 SmtA b0921 29.8 Protein...”
- Genome-scale analysis to the impact of gene deletion on the metabolism of E. coli: constraint-based simulation approach
Xu, BMC bioinformatics 2009 - “...b2615, b2746 b3729 b2747, b2763, b2764 b3730 b2927, b3041, b3058 b3967 b3177, b3187, b3360 b3972 b3368, b3634, b3639 b3804, b3805, b3850 b3974, b3990, b3991 b3992, b3993, b3994 b3997, b4039, b4040 b4407, s0001 SS MM APM GM MLM PPB genes b0159 b0159 b0175 b0185 b0522, b0523 b2687...”
- Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase.
Warren, FEBS letters 1990 (PubMed)- GeneRIF: N-terminus verified by Edman degradation on complete protein
- Clonal and antigenic analysis of serogroup A Neisseria meningitidis with particular reference to epidemiological features of epidemic meningitis in the People's Republic of China
Wang, Infection and immunity 1992 - “...IIb IIa, IIb IIa, IIb IIa, IIb I Z4727, Z4728, Z4729, Z4730, Z4731, Z4732, B54, B516, B236 B303 B328, B506 Z3510, Z3515 Z3771, Z3786, Z3787 Z3905, Z3909 B503,...”
- Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
Jahn, Microbial cell factories 2016 - “...ID Amplicon length Primer/probe sequence T m /C Length/bp cysG ( E. coli genome ) NJ74_RS16775 127 5-AGCCATTACTGAAACGACC-3 60.16 19 [ 46 ] 5-GCTGAATTTGTTGCAGTCC-3 60.16 19 5-FAM-ACCAACCAGCACCACTTCACCG-BHQ-1-3 69.99 22 oriT ( SEVA plasmids ) JX560321.2 104 5-CAGGTGCGAATAAGGGAC-3 60.25 18 [ 1 ] 5-GTAGACTTTCCTTGGTGTATCC-3 60.33 22 5-HEX-CCTATCCTGCCCGGCTGACG-BHQ-1-3...”
ESA_RS02410 siroheme synthase CysG from Cronobacter sakazakii ATCC BAA-894
53% identity, 97% coverage
- The Global Response of Cronobacter sakazakii Cells to Amino Acid Deficiency
Chen, Frontiers in microbiology 2018 - “...Thiamine phosphate synthase ESA_RS02415 cysD 6.83 Sulfate adenylyltransferase subunit 2 ESA_RS13090 amtB 6.79 Ammonium transporter ESA_RS02410 cysG 6.79 Siroheme synthase ESA_RS02425 cysC 6.78 Adenylyl-sulfate kinase ESA_RS07295 yciW 6.71 Oxidoreductase ESA_RS20880 N/A 6.69 Unknown ESA_RS20875 N/A 6.68 Unknown ESA_RS02420 cysN 6.6 Sulfate adenylyltransferase ESA_RS03740 cysA 6.52 Sulfate...”
cysG / P25924 uroporphyrinogen-III C-methyltransferase subunit (EC 4.99.1.4; EC 1.3.1.76) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 5 papers)
CYSG_SALTY / P25924 Siroheme synthase; EC 2.1.1.107; EC 1.3.1.76; EC 4.99.1.4 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
NP_462380 siroheme synthase from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
53% identity, 99% coverage
1pjqB / P25924 Structure and function of cysg, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis (see paper)
53% identity, 99% coverage
- Ligand: s-adenosyl-l-homocysteine (1pjqB)
YPO0158 siroheme synthase from Yersinia pestis CO92
52% identity, 99% coverage
YPTB0764 siroheme synthase, catalyses four separate reactions required for the transformation of uroporphyrinogen III into siroheme from Yersinia pseudotuberculosis IP 32953
55% identity, 97% coverage
- IscR is essential for yersinia pseudotuberculosis type III secretion and virulence
Miller, PLoS pathogens 2014 - “...3.7 YPTB0760 sulfite reductase, alpha subunit cysI 2.2 YPTB0761 3-phosphoadenosine 5-phosphosulfate (PAPS) reductase cysH 2.0 YPTB0764 Siroheme synthase cysG 2.4 YPTB0765 ATP-sulfurylase, subunit 2 cysD 4.8 YPTB0766 ATP-sulfurylase, subunit 1 cysN 4.0 YPTB0767 adenosine 5-phosphosulfate kinase cysC 2.7 YPTB2714 cysteine synthase A cysK 3.5 YPTB2732 ABC...”
SMDB11_RS00610 siroheme synthase CysG from Serratia marcescens subsp. marcescens Db11
55% identity, 96% coverage
- RNA‑seq analyses of antibiotic resistance mechanisms in Serratia marcescens
Li, Molecular medicine reports 2019 - “...where the most significant DE gene was located, which was the region from locus tag SMDB11_RS00610 to SMDB11_RS00625 (siroheme synthase, sulfate adenylyltransferase subunit 2, sulfate adenylyltransferase, and adenylyl-sulfate kinase) ( Fig. 3A ). The four genes were all identified as DEGs by edgeR, and the four...”
- “...IGV plot of two genomic regions. (A) IGV plot of the region from locus tag SMDB11_RS00610 to SMDB11_RS00625. (B) IGV plot of the genomic region from locus tag SMDB11_RS14095 to SMDB11_RS14110. IGV, Integrative Genomics Viewer. Figure 4. Functional enrichment analysis of DEGs. Count represents the number...”
ZMO0006 uroporphyrin-III C-methyltransferase from Zymomonas mobilis subsp. mobilis ZM4
50% identity, 99% coverage
- Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions
Yang, Frontiers in microbiology 2020 - “...in cysteine synthesis (CysC, N, D, H, I, J, K < ZMO0003-5, 0007-9, 0748>, and ZMO0006), and two ABC transporter (SsuA, C < ZMO1261, 2>) are involved in sulfonate import. The 56 chromosomal genes down-regulated by furfural in RMG were involved in amino acid metabolism and...”
- Transcriptome analysis of Zymomonas mobilis ZM4 reveals mechanisms of tolerance and detoxification of phenolic aldehyde inhibitors from lignocellulose pretreatment
Yi, Biotechnology for biofuels 2015 - “...2.53 3.60 2.47 Lipid metabolism ZMO1222 3-oxoacyl-ACP reductase 2.73 2.11 Metabolism of cofactors and vitamins ZMO0006 Uroporphyrin-III C-methyltransferase 3.09 2.05 Metabolism of cofactors and vitamins ZMO0474 3,4-dihydroxy-2-butanone 4-phosphate synthase 2.71 2.34 Metabolism of cofactors and vitamins ZMO1132 Lipoyl synthase 2.17 2.33 Metabolism of cofactors and vitamins...”
- Insights into acetate toxicity in Zymomonas mobilis 8b using different substrates
Yang, Biotechnology for biofuels 2014 - “...including five genes involved in cysteine synthesis (CysC, N, S, K<ZMO003, 4, 7, 0748>, and ZMO0006) and an ABC transport system SsuACB (ZMO1261-3) involved in sulfonate import. The 43 genes downregulated by acetate in RMG were involved in cell motility (for flagellar assembly) and protein synthesis...”
- Elucidation of Zymomonas mobilis physiology and stress responses by quantitative proteomics and transcriptomics
Yang, Frontiers in microbiology 2014 - “...to cysteine synthesis, with 6 genes clustered together ( cysD, I, J, K, N , ZMO0006 and ZMO0055) and tryptophan synthesis ( trpB, F ), and most of the downregulated genes encoded hypothetical proteins with unknown function with the exception of several related to flagellar biosynthesis...”
EAM_2691 siroheme synthase [includes: uroporphyrinogen-III C-methyltransferase; precorrin-2 dehydrogenase; sirohydrochlorin ferrochelatase] from Erwinia amylovora ATCC 49946
52% identity, 98% coverage
Kkor_0729 uroporphyrin-III C-methyltransferase from Kangiella koreensis DSM 16069
48% identity, 95% coverage
NMBNZ0533_1167 siroheme synthase CysG from Neisseria meningitidis NZ-05/33
51% identity, 95% coverage
BB1367 siroheme synthase from Bordetella bronchiseptica RB50
51% identity, 97% coverage
BP1055 siroheme synthase from Bordetella pertussis Tohama I
51% identity, 97% coverage
- Avirulent phenotype promotes Bordetella pertussis adaptation to the intramacrophage environment
Farman, Emerging microbes & infections 2023 - “...during infection. In particular, the sbp ( BP0966 ), cysD ( BP0970 ), cysG ( BP1055 ), cysJ ( BP1209 ), sulphate permease BP2049 , cysI ( BP3432 ), and adenosylhomocysteinase BP3068 genes were significantly downregulated. The BP2816BP2818 locus, which encodes the putative methionine importer, was...”
- Combined RNAseq and ChIPseq Analyses of the BvgA Virulence Regulator of Bordetella pertussis
Coutte, mSystems 2020 - “...BvgA mutant versus a BP536 BvgA/S double mutant. As an example of an annotation error, bp1055 was considered vag by Moon et al. ( 7 ) but not in this study because of reads overlapping from the 3 end of the vag bp1054 ( prn )...”
- Differentially expressed genes in Bordetella pertussis strains belonging to a lineage which recently spread globally
de, PloS one 2014 - “...BP0970A cysD sulfate adenylyltransferase subunit 2 C BP0971 cysN sulfate adenylyltransferase subunit 1 (Pseudogene) Un BP1055 cysG siroheme synthase C 4.7 * 3.2 BP1362 amino-acid ABC transporter, ATP-binding protein Cm 3.6 * 3.4 BP1363 amino-acid ABC transporter, permease protein Cm 6.2 * 4.4 3.2 BP1364 amino-acid...”
NMV_1233 siroheme synthase CysG from Neisseria meningitidis 8013
51% identity, 96% coverage
XF0832 siroheme synthase from Xylella fastidiosa 9a5c
45% identity, 96% coverage
- Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques
Baldi, Frontiers in plant science 2017 - “...CGGGTTCCAAAGGATTCCTAA NADH-ubiquinone oxidoreductase, NQO14 subunit gltT a XF0656 TTGGGTGTGGGTACGTTGCTG CGCTGCCTCGTAAACCGTTGT Glutamate symport protein cysG a XF0832 GGCGGCGGTAAGGTTG GCGTATGTCTGTGCGGTGTGC cysG Siroheme synthase petC a XF0910 CTGCCATTCGTTGAAGTACCT CGTCCTCCCAATAAGCCT Ubiquinol cytochrome c oxidoreductase pilU a XF1632 CAATGAAGATTCACGGCAATA ATAGTTAATGGCTCCGCTATG Twitching motility protein leuA a XF18188 GGGCGTAGACATTATCGAGAC GTATCGTTGTGGCGTACACTG 2-Isopropylmalate synthase lacF...”
- “...CCTCGGGTCATCACATAAGGC ABC transporter sugar permease rfbD b XF0257 TTTGGTGATTGAGCCGAGGGT a TCCATAAACGGCGCCTTC dTDP-4-dehydrorhamnose-3, 5-epimerase cysG b XF0832 GGCGGCGGTAAGGTTG a GCATATGTCTGTGCGGTGTGC cysG Siroheme synthase holC c XF0136 ATGGCACGCGCCGACTTCT ATGTCGTGTTTGTTCATGTGCAGG DNA polymerase III holoenzyme, chi subunit nuoL c XF0316 TAGCGACTTACGGTTACTGGGC ACCACCGATCCACAACGCAT NADH-ubiquinone oxidoreductase, NQO12 subunit gltT c XF0656 TCATGATCCAAATCACTCGCTT...”
- A multigene phylogenetic study of clonal diversity and divergence in North American strains of the plant pathogen Xylella fastidiosa
Schuenzel, Applied and environmental microbiology 2005 - “...XF0316 nuoL XF0318 nuoN XF0656 gltT XF0832 cysG DNA polymerase III holoenzyme chi subunit dTDP-4-dehydrorhamnose 3,5-epimerase NADH-ubiquinone oxidoreductase,...”
- Multilocus sequence type system for the plant pathogen Xylella fastidiosa and relative contributions of recombination and point mutation to clonal diversity
Scally, Applied and environmental microbiology 2005 - “...gltT cysG XF0316 Yes Yes petC nuoN XF0656 XF0832 Yes pilU leuA lacF XF0318 XF0910 Yes Yes DNA polymerase III holoenzyme, chi subunit dTDP-4-dehydrorhamnose-3,...”
PD1840 siroheme synthase from Xylella fastidiosa Temecula1
45% identity, 95% coverage
- Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques
Baldi, Frontiers in plant science 2017 - “...GCATTGGCAGCAACACTCGAATCA Outer membrane afimbrial adhesin gltT e PD1516 TTTTTCAGGGGTGTCGCGC TTCCAACGTTACTGGACGCT Glutamate symport protein cysG e PD1840 CCAAACATAGAAGCACGCCG CGTATGTCTGTGCGGTGTG Siroheme synthase leuA e PD1047 GGCCAGTGCTGTGTTTTGTT GGGCTACTTGCTGGAGGAAG 2-Isopropylmalate synthase lacF e PD1465 TTCTTTGGTGGGTTGGGTGT CACACAGCATCAACGTCGTC ABC transporter sugar permease a Schuenzel et al. ( 2005 ) . b Almeida...”
- The Secreted Protease PrtA Controls Cell Growth, Biofilm Formation and Pathogenicity in Xylella fastidiosa
Gouran, Scientific reports 2016 - “...asd (PD0608), aspH (PD0777), trpDE (PD0876 and PD0877), serC (PD1358), leuB (PD1397), cofactor recycling cysG (PD1840), folP (PD0068), ptr1 (PD0677), nadA (PD0869), grx (PD1409), ribF (PD1438), folB (PD1642), terpenoid dxr (PD0328), phosphate uptake oprO (PD0270), ammonium uptake amtB (PD1024), sugar ABC transporter malGFE (PD1464-6), and energy...”
SMc01053 PROBABLE SIROHEME SYNTHASE PROTEIN from Sinorhizobium meliloti 1021
41% identity, 93% coverage
BCAN_A0184 uroporphyrin-III C-methyltransferase from Brucella canis ATCC 23365
43% identity, 89% coverage
O50477 precorrin-2 dehydrogenase (EC 1.3.1.76) from Rhizobium etli (see paper)
41% identity, 95% coverage
BMEI1768 UROPORPHYRIN-III C-METHYLTRANSFERASE / PRECORRIN-2 OXIDASE (EC 1.-.-.-) / FERROCHELATASE (EC 4.99.1.-) from Brucella melitensis 16M
43% identity, 89% coverage
BAbS19_I01700 CysG, siroheme synthase from Brucella abortus S19
43% identity, 89% coverage
- Comparative proteomic and genomic analyses of Brucella abortus biofilm and planktonic cells
Tang, Molecular medicine reports 2020 - “...groups [GO:0016746] nitrogen compound metabolic process [GO:0006807] 5.3 2.02 BAbS19_I14530 MaoC-like dehydratase Butanoate 6.5 1.58 BAbS19_I01700 CysG, siroheme synthase NAD binding [GO:0051287]; precorrin-2 dehydrogenase activity [GO:0043115]; sirohydrochlorin ferrochelatase activity [GO:0051266]; uroporphyrin-III C-methyltransferase activity [GO:0004851] cobalamin biosynthetic process [GO:0009236]; siroheme biosynthetic process [GO:0019354] metabolism [path:ko00650] 5.7 2.07...”
BAB1_0179 Uroporphyrin-III C/tetrapyrrole (Corrin/Porphyrin) methyltransferase:Pyrokinin:Uroporphiryn-III C-methyltransferase:Uroporphy... from Brucella melitensis biovar Abortus 2308
43% identity, 89% coverage
VF_0773 uroporphyrin-III C-methyltransferase from Vibrio fischeri ES114
VF_0773 uroporphyrinogen-III C-methyltransferase from Aliivibrio fischeri ES114
57% identity, 52% coverage
- Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes
Wasilko, Molecular microbiology 2021 - “...65.15% VF_0323 CysC CysH PAPS sulfotransferase 99% 69.96% VF_0312 CysH CysG Siroheme synthase 54% 54% VF_0773 CobA 58% 33.46% VF_1531 VF_1531 CysI NADPH-sulfite reductase hemoprotein 97% 70.97% VF_0311 CysI CysJ NADPH-sulfite reductase flavoprotein 98% 53.18% VF_0310 CysJ CysE Serine transacetylase 97% 72.83% VF_2347 CysE CysM O...”
- “...cysD Sulfate adenylyltransferase 1 - VF_0322 yfbS Divalent anion: sodium symporter family protein 2 - VF_0773 cobA Urophorphyrin-III C-methyltransferase 3 - VF_1008 yfcA Inner membrane protein 8 + VF_1531 VF_1531 Ferrochelatase 3 - VF_1893 cysK O -Acetyl (thiol)-lyase A 3 - a Mutants were assessed for...”
BC1426 Uroporphyrin-III C-methyltransferase from Bacillus cereus ATCC 14579
49% identity, 54% coverage
Dshi_1155 uroporphyrin-III C-methyltransferase from Dinoroseobacter shibae DFL 12
41% identity, 96% coverage
PA14_06660 putative uroporphyrin-III c-methyltransferase from Pseudomonas aeruginosa UCBPP-PA14
59% identity, 51% coverage
SO3728 uroporphyrin-III C-methyltransferase from Shewanella oneidensis MR-1
54% identity, 53% coverage
P95417 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Pseudomonas aeruginosa (see 2 papers)
ACG06_RS02660 uroporphyrinogen-III C-methyltransferase from Pseudomonas aeruginosa
G3XD80 uroporphyrinogen-III C-methyltransferase from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PA0510 probable uroporphyrin-III c-methyltransferase from Pseudomonas aeruginosa PAO1
59% identity, 51% coverage
- Whole-transcriptome analysis reveals mechanisms underlying antibacterial activity and biofilm inhibition by a malic acid combination (MAC) in Pseudomonas aeruginosa
Song, PeerJ 2023 - “...FC = 7.28), FMN-dependent NADH-azoreductase AzoR2 ( ACG06_RS16675 , FC = 7.27), uroporphyrinogen-III C-methyltransferase ( ACG06_RS02660 , FC = 6.38), tryptophan synthase subunit beta ( ACG06_RS00215 , FC = 6.37), MBL fold metallo-hydrolase ( ACG06_RS12740 , FC = 6.36), NADP-dependent glyceraldehyde-3-phosphate dehydrogenase ( ACG06_RS14485 , FC...”
- Proteome-wide identification of druggable targets and inhibitors for multidrug-resistant <i>Pseudomonas aeruginosa</i> using an integrative subtractive proteomics and virtual screening approach
Vemula, Heliyon 2025 - Pseudomonas aeruginosa and Staphylococcus aureus Display Differential Proteomic Responses to the Silver(I) Compound, SBC3
Piatek, Antibiotics (Basel, Switzerland) 2023 - “...binding protein component of ABC transporter Q9HVS5 Peptide transmembrane transporter activity 10.6 PA0510 Uroporphyrinogen-III C-methyltransferase G3XD80 Methyltransferase; cobalamin biosynthesis; porphyrin biosynthesis 11.7 nirQ Denitrification regulatory protein NirQ Q51481 Transcription; transcription regulation 13.8 rmf Ribosome modulation factor Q9HZF9 Translation regulation 36.7 PA2462 Haemagg_act domain-containing protein Q9I120 Macromolecule...”
- Pseudomonas aeruginosa and Staphylococcus aureus Display Differential Proteomic Responses to the Silver(I) Compound, SBC3
Piatek, Antibiotics (Basel, Switzerland) 2023 - “...10.5 PA4496 Probable binding protein component of ABC transporter Q9HVS5 Peptide transmembrane transporter activity 10.6 PA0510 Uroporphyrinogen-III C-methyltransferase G3XD80 Methyltransferase; cobalamin biosynthesis; porphyrin biosynthesis 11.7 nirQ Denitrification regulatory protein NirQ Q51481 Transcription; transcription regulation 13.8 rmf Ribosome modulation factor Q9HZF9 Translation regulation 36.7 PA2462 Haemagg_act domain-containing...”
- Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin-rifampicin combination against Pseudomonas aeruginosa
Mahamad, Journal of biomedical science 2022 - “...extrusion protein 3.11 <0.05 narK1 Nitrite extrusion protein 3.18 <0.05 nirN Cytochrome C 2.51 2.24E07 PA0510 Uroporphyrin-III C-methyltransferase 2.99 3.93E08 nirJ , nirH , nirG , nirL , nirD , nirF Heme d1 biosynthesis protein <1.95 to 3.15 0.001 nirC Cytochrome c55X 2.26 5.33E06 nirM Cytochrome...”
- ECF Sigma Factor HxuI Is Critical for In Vivo Fitness of Pseudomonas aeruginosa during Infection
Cai, Microbiology spectrum 2022 - “...Cytochrome C oxidase assembly factor 2.21 6.07E-10 PA0509 nirN Cytochrome C Anr box 2.61 4.52E-38 PA0510 nirE Uroporphyrin-III C-methyltransferase Anr box 2.67 3.9E-30 PA0511 nirJ Heme d1 biosynthesis protein Anr box 2.32 1.18E-28 PA0512 nirH Heme d1 biosynthesis protein Anr box 2.25 2.72E-31 PA0513 nirG Heme...”
- A High-Throughput Method for Identifying Novel Genes That Influence Metabolic Pathways Reveals New Iron and Heme Regulation in Pseudomonas aeruginosa
Glanville, mSystems 2021 - “...biosynthesis 2 154 nirL PA0514 HemD1 biosynthesis 154 nirJ PA0511 HemD1 biosynthesis 1 154 nirE PA0510 Uroporphyrin III c -methyltransferase 1 154 Siroheme cysG PA2611 Siroheme synthase 155 cobA PA1778 Uroporphyrin III methyltransferase 1 155 Colbamin (B12) cobI PA2904 Cobalt-factor-2 C20-methyltransferase 156 cobG PA2906 Precorrin-3B synthase...”
- Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype
Thöming, NPJ biofilms and microbiomes 2020 - “...PA14_38860 PA1982 exaA 5.8 PA14_06650 PA0509 nirN 3.0 PA14_07355 PA0565 3.6 PA14_38880 PA1981 5.7 PA14_06660 PA0510 nirE 3.3 PA14_09980 PA4167 dkgB 2.7 PA14_38900 PA1980 exaE 2.9 PA14_06670 PA0511 nirJ 3.5 PA14_10170 PA4159 fepB 3.2 PA14_38910 PA1979 exaD 3.0 PA14_06680 PA0512 nirH 3.2 PA14_10200 PA4156 2.8 PA14_38920...”
- Unraveling the role of quorum sensing-dependent metabolic homeostasis of the activated methyl cycle in a cooperative population of Burkholderia glumae
Kang, Scientific reports 2019 - “...BP1026B_I2850 - BP1026B_II1162 Pseudomonas aeruginosa PAO1 d PA0432* PA0430* PA1927* PA1843* PA0546 - PA4209 - PA0510 a Gene IDs and regulations by QS of B . glumae BGR1 were obtained from An et al . 32 . Bold letters indicate genes activated by QS. b Gene...”
- Protein complex formation during denitrification by Pseudomonas aeruginosa
Borrero-de, Microbial biotechnology 2017 - “...via NorC (Fig. 2 A). NosR was, itself, found to interact with NarG, NarH, NirE (PA0510), NirQ, NirS and NirM. Importantly, the N 2 O reductase NosZ (PA3392) exhibited a strong interaction with NosR. The thus far not well understood NosR function seems to be linked...”
- “...Furthermore, the haem d 1 biosynthesis proteins NirF (PA0516), NirJ (PA0511), NirL (PA0514) and NirE (PA0510) were found attached (Layer etal ., 2010 ). Haem d 1 is a cofactor of nitrite reductase NirS. The electron donor systems for NirS, the c type cytochromes NirM (PA0518)...”
- Effect of efflux pump inhibition on Pseudomonas aeruginosa transcriptome and virulence
Rampioni, Scientific reports 2017 - “...a Gene name a Fold change b Product name a PA0509 * nirN 2.27 NirN PA0510 * nirE 2.33 NirE PA0511 * nirJ 2.23 heme d 1 biosynthesis protein NirJ PA0514 * nirL 2.3 heme d 1 biosynthesis protein NirL PA0516 * nirF 2.3 heme d...”
- More
SM39_1886 uroporphyrinogen-III C-methyltransferase from Serratia marcescens SM39
58% identity, 52% coverage
Dred_2162 uroporphyrin-III C-methyltransferase from Desulfotomaculum reducens MI-1
50% identity, 48% coverage
XAC3340 siroheme synthase from Xanthomonas axonopodis pv. citri str. 306
57% identity, 53% coverage
- The sulfur/sulfonates transport systems in Xanthomonas citri pv. citri
Pereira, BMC genomics 2015 - “...domains of S. typhimurium CysG and the three CysG from X. citri, showed that only Xac3340 presented the necessary motifs for the predicted activity (Additional file 7 : Figure A2-B). Although Xac3340 encodes an enzyme that fits better in the model of a siroheme synthase, the...”
- “...of transcripts were not suitable for these genes. The gene that encodes the putative CysG (Xac3340) was not identified. Similarly, we tried to amplify the additional cysG genes without success. Still in XAM1, we observed the amplification of cysB that shares 39% of amino acid sequence...”
SXYL_02635 uroporphyrinogen-III C-methyltransferase from Staphylococcus xylosus
50% identity, 52% coverage
- Investigating Extracellular DNA Release in Staphylococcus xylosus Biofilm In Vitro
Leroy, Microorganisms 2021 - “...Energy-coupling factor transporter 2.2 * SXYL_01194 hemA Glutamyl-tRNA reductase 2.0 SXYL_01196 hemC Porphobilinogen deaminase 2.5 SXYL_02635 cobA Uroporphyrin-III C-methyltransferase 2.0 2.9 SXYL_00839 thiD Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase 2.0 SXYL_01231 HesA/MoeB/ThiF family protein 2.1 2.0 SXYL_01893 menF Isochorismate synthase 2.0 2.4 SXYL_02295 folE2 GTP cyclohydrolase FolE2 2.2 INORGANIC ION...”
2yboA / P95417 The x-ray structure of the sam-dependent uroporphyrinogen iii methyltransferase nire from pseudomonas aeruginosa in complex with sah (see paper)
58% identity, 51% coverage
- Ligand: s-adenosyl-l-homocysteine (2yboA)
SUMT_PRIMG / P29928 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Priestia megaterium (Bacillus megaterium) (see paper)
COBA / AAA22317.1 S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Bacillus megaterium (see paper)
53% identity, 51% coverage
- function: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and siroheme.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
subunit: Monomer.
B8FVX9 uroporphyrinogen-III C-methyltransferase from Desulfitobacterium hafniense (strain DSM 10664 / DCB-2)
50% identity, 49% coverage
LMOf2365_1210 uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase from Listeria monocytogenes str. 4b F2365
49% identity, 49% coverage
BTB_RS10815 uroporphyrin-III C-methyltransferase from Bacillus thuringiensis Bt407
47% identity, 51% coverage
lmo1201 similar uroporphyrinogen-III methyltransferase/uroporphyrinogen-III synthase from Listeria monocytogenes EGD-e
LMOf6854_1240 uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase from Listeria monocytogenes str. 1/2a F6854
49% identity, 49% coverage
LM6179_1508 uroporphyrinogen-III C-methyltransferase from Listeria monocytogenes 6179
49% identity, 49% coverage
BC2134 Uroporphyrin-III C-methyltransferase from Bacillus cereus ATCC 14579
BMB171_RS10580 uroporphyrin-III C-methyltransferase from Bacillus thuringiensis BMB171
47% identity, 51% coverage
- SecDF as part of the Sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins
Vörös, PloS one 2014 - “...(operons BC3941-3944; BC0695-0698) including a cytochrome d ubiquinol oxidase (BC4792-4793), fermentation (BC0491-0492, BC2220), anaerobic respiration (BC2134, BC2128) and the regulator of the arginine deaminase operon arcABDC (BC0410). The genome of B. cereus ATCC 14579 also contains a cryptic, linear plasmid pBClin15, encoding what appears to be...”
- Global gene expression profile for swarming Bacillus cereus bacteria
Salvetti, Applied and environmental microbiology 2011 - “...BC0407 BC0408 BC0409 BC0410 BC0491 BC0492 BC2220 BC0668 BC2134 BC2128 Virulence Other a BC3526 BC3698 BC3101 BC0753 BC0754 BC0755 BC0756 BC1435 BC1436 Gene name...”
- “...phosphorylation (BC4792), and anaerobic respiration (BC2128 and BC2134), were downregulated in the swarming compared to the nonswarming population (Table 1; see...”
- Transcriptional responses of Bacillus cereus towards challenges with the polysaccharide chitosan
Mellegård, PloS one 2011 - “...chitosan A treatment. locus tag Expression ratio a Significance(p-value) b annotation c feature d Downregulated BC2134 0.3 10 4 bifunctional uroporphyrinogen-III methylase domain BC0744 0.3 10 5 hydroxymethylpyrimidine transport system permease protein SS, TMS(6) BC4927 0.3 10 5 cell surface protein TMS(2) BC3855 0.3 10 4...”
- NupR Responding to Multiple Signals Is a Nucleoside Permease Regulator in Bacillus thuringiensis BMB171
Qin, Microbiology spectrum 2022 - “...to increased expression of genes coding for cobalamin biosynthesis protein (BMB171_RS10575) and bifunctional uroporphyrinogen methyltransferase (BMB171_RS10580). The absence of nupR also indirectly affects sugar utilization and transport. The expression of cggR (BMB171_RS25590, central glycolytic genes regulator), gap2 (BMB171_RS25585, glyceraldehyde-3-phosphate dehydrogenase) modulated by CggR ( 13 ),...”
Q50EH6 uroporphyrinogen-III synthase (EC 4.2.1.75) from Limosilactobacillus reuteri (see paper)
50% identity, 52% coverage
Cthe_2528 uroporphyrinogen-III synthase / uroporphyrinogen-III C-methyltransferase from Clostridium thermocellum ATCC 27405
Cthe_2528 uroporphyrinogen-III C-methyltransferase from Acetivibrio thermocellus ATCC 27405
49% identity, 49% coverage
- Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution
Ha-Tran, Microorganisms 2021 - “...Cthe_3014, Cthe_3018, Cthe_3020, Cthe_0428, Cthe_0429, Cthe_0430), ammonia assimilation genes (Cthe_01970199), porphyrin biosynthesis gene (Cthe_2525, Cthe_2527, Cthe_2528, Cthe_2529), and sulfate transport (Cthe_2533). The results suggest that FAs1 and GAs1 did not suffer much from redox imbalance as hydrogen production was favorably maintained for cell redox homeostasis. Transcriptomic...”
NASF_BACSU / P42437 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Bacillus subtilis (strain 168) (see 2 papers)
48% identity, 51% coverage
DCF50_p2935 uroporphyrinogen-III C-methyltransferase from Dehalobacter sp. CF
48% identity, 48% coverage
Glov_3656 uroporphyrin-III C-methyltransferase from Geobacter lovleyi SZ
37% identity, 97% coverage
SUMT_BACSU / O34744 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Bacillus subtilis (strain 168) (see paper)
49% identity, 52% coverage
- function: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and siroheme.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
Q6TA16 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Geobacillus stearothermophilus (see paper)
49% identity, 53% coverage
SHALO_1527, SJPD1_1540, SMN_1549, SMUL_1563 uroporphyrinogen-III C-methyltransferase from Sulfurospirillum halorespirans DSM 13726
44% identity, 49% coverage
- Tetrachloroethene respiration in Sulfurospirillum species is regulated by a two-component system as unraveled by comparative genomics, transcriptomics, and regulator binding studies
Esken, MicrobiologyOpen 2020 - “...SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike SHALO_1530 SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic...”
- “...cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike SHALO_1530 SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic acid A,Cdiamide synthase CbiA SHALO_1531 SMUL_1567 SMN_3265 SJPD1_1543 cysteinerich CWC protein SHALO_1532...”
- “...SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike SHALO_1530 SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic acid A,Cdiamide...”
- “...SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike SHALO_1530 SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic acid...”
- “...CbiL 6.826321327 3.45861E65 SMUL_1560 CbiF 7.12898108 2.37995E69 SMUL_1561 CbiG 6.327298396 5.61016E85 SMUL_1562 CbiH 6.783888426 2.94934E59 SMUL_1563 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase 5.675055838 1.15417E79 SMUL_1564 CbiJ 4.857598806 6.31887E71 SMUL_1565 lipid A export ATPbinding/permease protein MsbAlike 3.373444956 2.04003E15 SMUL_1566 CbiA 4.123923514 6.67467E31 SMUL_1567 cysteinerich CWC protein 3.537696231 1.02731E22...”
D2KI47 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Hordeum vulgare (see paper)
50% identity, 53% coverage
F2CPW6 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Hordeum vulgare (see paper)
50% identity, 53% coverage
UPM1_ARATH / Q42606 S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase, chloroplastic; Urophorphyrin III methylase; Urophorphyrin methylase 1; AtUPM1; EC 2.1.1.107 from Arabidopsis thaliana (Mouse-ear cress) (see 5 papers)
AT5G40850 UPM1 (UROPHORPHYRIN METHYLASE 1); uroporphyrin-III C-methyltransferase from Arabidopsis thaliana
NP_198901 urophorphyrin methylase 1 from Arabidopsis thaliana
51% identity, 51% coverage
- function: Essential protein required for siroheme biosynthesis (PubMed:20592802). Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1 (PubMed:9006913). It is a step in the biosynthesis of siroheme (PubMed:9006913). Promotes nitrogen and sulfur assimilation as well as photosynthesis efficiency by triggering chlorophyll, nitrite reductase (NiR) and sulfite reductase (SiR) biosynthesis (PubMed:29472934).
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
disruption phenotype: Embryo lethal. - A comprehensive analysis of transcriptomic data for comparison of plants with different photosynthetic pathways in response to drought stress
Karami, PloS one 2023 - “...genes HEMA2 (AT1G09940), ferrochelatase 2 ( FC2 ; AT2G30390) and urophorphyrin methylase 1 ( UPM1; AT5G40850) were also detected only in C3 plants. The up-regulated gene protochlorophyllide oxidoreductase A ( PORA ; AT5G54190) and down-regulated genes coproporphyrinogen III oxidase (AT1G03475) and, chlorophyll synthase ( G4 ;...”
- The transcription factor WRKY22 is required during cryo-stress acclimation in Arabidopsis shoot tips
Stock, Journal of experimental botany 2020 - “...protein 33 AT1G02820 3.8 LEA3 development 34 AT3G06900 3.8 U4 SMALL NUCLEOLAR RNA2 RNA.processing 35 AT5G40850 3.8 UROPHORPHYRIN METHYLASE 1 tetrapyrrole synthesis 36 AT1G51850 3.8 Leucine-rich repeat protein kinase family protein signalling.receptor kinases.misc 37 AT4G33070 3.7 ATPDC1 fermentation.PDC 38 AT1G24280 3.7 G6PD3 OPP.oxidative 39** AT5G23010 3.7...”
- Transcriptome Profile of the Variegated Ficus microcarpa c.v. Milky Stripe Fig Leaf
Shih, International journal of molecular sciences 2019 - “...of the cytochrome b6f complex ndhb.2 3.0 ATCG01250 NADH dehydrogenase ND2 Tetrapyrrole metabolism upm1 3.2 AT5G40850 Urophorphyrin III methylase nyc1 2.4 AT4G13250 Chlorophyll b reductase clh1 4.7 AT1G19670 Chlorophyllase I clh2 4.6 AT5G43860 Chlorophyllase II nye1 3.1 AT4G22920 Non-yellowing 1, stay-green 1 sgrl 3.7 AT1G44000 Stay-green...”
- Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways
Pavlů, International journal of molecular sciences 2018 - “...2 [ 157 ] NIR1 AT2G15620 Ferredoxinnitrite reductase 1/0 [ 13 , 158 ] UMP1 AT5G40850 Urophorphyrin methylase 1 [ 13 , 157 , 158 ] GLN1-1 AT5G37600 Glutamine synthetase cytosolic isozyme 1-1 [ 159 , 164 ] GLN1-4 AT5G16570 Glutamine synthetase cytosolic isozyme 1-4 0/1...”
- Alleviation of Nitrogen and Sulfur Deficiency and Enhancement of Photosynthesis in Arabidopsis thaliana by Overexpression of Uroporphyrinogen III Methyltransferase (UPM1)
Garai, Frontiers in plant science 2017 - “...The AtUPM1 coding DNA sequence (CDS) (accession # NM_123450 ) of the complete AtUPM1 gene (At5g40850), consisting of 1,110 bp, was amplified from the cDNA library of WT A. thaliana (Col 0), using the gene specific primer pair: F-5ATGGCTCTTGTTCAGCGGATTC3 and R - 5CTACCGGGTCTCTACAA GGCA 3; it...”
- Phylogenetic analysis and photoregulation of siroheme biosynthesis genes: uroporphyrinogen III methyltransferase and sirohydrochlorin ferrochelatase of Arabidopsis thaliana
Garai, Physiology and molecular biology of plants : an international journal of functional plant biology 2016 - “...for uroporphyrinogen III methyl transferase: promoter ID (At5g40850) GATA promoter motif [LRE] 16381472 16381477 tgatag GATA promoter motif [LRE] 16381480...”
- The immediate upstream region of the 5'-UTR from the AUG start codon has a pronounced effect on the translational efficiency in Arabidopsis thaliana
Kim, Nucleic acids research 2014 - “...qRT-PCR using specific primers for the GFP coding region. Although the 5-UTRs of AT1G58420 and AT5G40850 induced dramatic differences in the GFP level, these constructs showed no significant differences in the transcript level, confirming that the difference in the GFP level stems from the difference in...”
- “...103 CATTTTTCAATTTCATAAAAC 24 Pre-mRNA-splicing factor 38B 148 AT4G11320 1 TTACTTTTAAGCCCAACAAAA 24 Probable cysteine proteinase 82 AT5G40850 10 GGCGTGTGTGTGTGTTGTTGA 19 Urophorphyrin III methylase 304 AT1G06150 9 GTGGTGAAGGGGAAGGTTTAG 14 Transcription factor EMB1444 503 AT2G26080 19 TTGTTTTTTTTTGGTTTGGTT 10 Glycine dehydrogenase 42 Nucleotide sequences were obtained from 5-UTRs (nucleotide positions...”
- Systems analysis of transcriptome data provides new hypotheses about Arabidopsis root response to nitrate treatments
Canales, Frontiers in plant science 2014 - “...factor family protein 16 16 0 At1g80380 P-loop containing nucleoside triphosphate hydrolases 16 16 0 At5g40850 Urophorphyrin methylase 1 ( UPM1 ) 15 15 0 At5g10210 Unknown 15 15 0 At1g77760 Nitrate reductase 1 ( NIA1 ) 15 15 0 At1g78050 Phosphoglycerate/bisphosphoglycerate mutase ( PGM )...”
- More
- Genome wide transcriptional profile analysis of Vitis amurensis and Vitis vinifera in response to cold stress
Xin, PloS one 2013 - “...GSVIVT01031881001 XP_002519124 GTP-binding protein alpha subunit, gna, putative [Ricinus communis] 11 16 3 2 GSVIVT01032030001 NP_198901 urophorphyrin methylase 1 [Arabidopsis thaliana] 13 9 3 4 GSVIVT01033502001 AEI60128 microsomal delta-12 oleate desaturase [Vitis labrusca] 3 1 2 3 GSVIVT01034054001 NP_001044811 Os01g0850000 [Oryza sativa Japonica Group] 5 5...”
SYNW2475 putative uroporphyrin-III c-methyltransferase from Synechococcus sp. WH 8102
51% identity, 50% coverage
NP_001105724 siroheme uroporphyrinogen methyltransferase 1 from Zea mays
49% identity, 53% coverage
GSU3286 uroporphyrinogen III synthase/methyltransferase from Geobacter sulfurreducens PCA
52% identity, 47% coverage
Q5KZ09 uroporphyrinogen-III C-methyltransferase from Geobacillus kaustophilus (strain HTA426)
46% identity, 53% coverage
HVO_0077 uroporphyrin-III C-methyltransferase from Haloferax volcanii DS2
51% identity, 52% coverage
- Nitrate-Responsive Suppression of Dimethyl Sulfoxide Respiration in a Facultative Anaerobic Haloarchaeon, Haloferax volcanii
Koyanagi, Journal of bacteriology 2021 (secret) - Growth phenotype analysis of heme synthetic enzymes in a halophilic archaeon, Haloferax volcanii
Kosugi, PloS one 2017 - “...H . volcanii genome. In contrast, the genes included in the anaerobic Ahb pathway, hemX (HVO_0077), cysG (HVO_2312), nirDL and nirGH (HVO_2227 and HVO_2313, respectively. Both are homologous to ahbAB ), AhbC (HVO_1121), and ahbD (HVO_2144), were completely conserved. Grey and white arrows in the figure...”
Synpcc7942_2610 uroporphyrin-III C-methyltransferase from Synechococcus elongatus PCC 7942
47% identity, 53% coverage
GRMZM2G000739 urophorphyrin methylase 1 from Zea mays
48% identity, 53% coverage
Amuc_0896 uroporphyrin-III C-methyltransferase from Akkermansia muciniphila ATCC BAA-835
46% identity, 48% coverage
- Comparative Genomics Guides Elucidation of Vitamin B12 Biosynthesis in Novel Human-Associated Akkermansia Strains
Kirmiz, Applied and environmental microbiology 2020 - “...most of the genes associated with the upstream (tetrapyrrole precursor biosynthesis, e.g., Amuc_0090, Amuc_0091, Amuc_0417, Amuc_0896, and Amuc_1730) and downstream (nucleotide loop assembly, e.g., Amuc_1678 to Amuc_1683) stages of vitamin B 12 biosynthesis ( 28 ). Genes annotated as a TonB-dependent transporter (e.g., Amuc_1684) and an...”
SUMT_METBF / Q46BL0 S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase; Mba_SUMT; SUMT; Uroporphyrinogen-III C-methyltransferase; EC 2.1.1.107 from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
49% identity, 51% coverage
- function: Involved in the archaeal biosynthesis of heme. Catalyzes the methylation of carbons 2 and 7 of uroporphyrinogen-III (UROGEN) to yield precorrin-2. It does not catalyze the overmethylation of precorrin-2 to trimethylpyrrocorphin.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
subunit: Homodimer.
CAETHG_1125 uroporphyrinogen-III C-methyltransferase from Clostridium autoethanogenum DSM 10061
42% identity, 50% coverage
CC_1118 uroporphyrinogen-III C-methyltransferase from Caulobacter vibrioides CB15
50% identity, 53% coverage
1s4dE / P21631 Crystal structure analysis of the s-adenosyl-l-methionine dependent uroporphyrinogen-iii c-methyltransferase sumt (see paper)
46% identity, 54% coverage
- Ligand: s-adenosyl-l-homocysteine (1s4dE)
cobA / P21631 CobA from Sinorhizobium sp. (see 2 papers)
SUMT_SINSX / P21631 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Sinorhizobium sp. (see 2 papers)
45% identity, 55% coverage
- function: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and siroheme. Neither uroporphyrin III nor the chlorin (factor I) is a substrate of SUMT.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
catalytic activity: uroporphyrinogen III + S-adenosyl-L-methionine = precorrin-1 + S-adenosyl-L-homocysteine + H(+) (RHEA:19089)
catalytic activity: precorrin-1 + S-adenosyl-L-methionine = precorrin-2 + S- adenosyl-L-homocysteine (RHEA:21972)
subunit: Homodimer.
disruption phenotype: Cells lacking this gene are deficient in cobalamin biosynthesis.
sll0378 uroporphyrin-III C-methyltransferase from Synechocystis sp. PCC 6803
Q55749 Uroporphyrinogen-III C-methyltransferase from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa)
45% identity, 52% coverage
- Self-Assembly of Nanofilaments in Cyanobacteria for Protein Co-localization
Zedler, ACS nano 2023 - “...2 hydration protein 2.54 Q55558 sll0172 Unknown protein 2.04 P73005 slr1041 PatA subfamily 2.34 Q55749 sll0378 Uroporphyrinogen-III C-methyltransferase 2.50 In both nanofilament-forming strains, proteins involved in carbon fixation were found to be the most upregulated ( Tables 1 and 2 ). These include carboxysome shell proteins...”
- “...nitrogen stress were not as pronounced, with only a single protein involved in cofactor biosynthesis (Sll0378) significantly downregulated. Accordingly, no differences were observed in the amount of phycocyanin and chlorophyll a ( Supplementary Figure 5B,D ). In cyanobacteria, carbon and nitrogen metabolism are sinks for ATP...”
- Photorespiratory 2-phosphoglycolate metabolism and photoreduction of O2 cooperate in high-light acclimation of Synechocystis sp. strain PCC 6803
Hackenberg, Planta 2009 - “...htrA , Protease slr2048 2.53 0.24 Unknown proteinTPR-motif, periplasmic sll0857 4 2.49 0.10 Unknown protein sll0378 2.46 1.83 cysG , cobA , Uroporphyrin-III C-methyltransferase sll1514 2.46 0.16 hspA , hsp1 , 16.6kDa small heat shock protein sll0856 4 2.34 0.60 sigH , rpoE , Group3 RNA...”
- Cobalamin (vitamin B12) biosynthesis: identification and characterization of a Bacillus megaterium cobI operon
Raux, The Biochemical journal 1998 - “...(ET ) slr1368 (T ) slr1879 slr0239 slr0969 (N-ter) sll1501 sll0378 36.1 cobA 37.5 cobO 37.7 slr0260 cbiL cbiF cbiG cbiA cysGA cbiY btuR Figure 2 M. jannaschii...”
- Self-Assembly of Nanofilaments in Cyanobacteria for Protein Co-localization
Zedler, ACS nano 2023 - “...CO 2 hydration protein 2.54 Q55558 sll0172 Unknown protein 2.04 P73005 slr1041 PatA subfamily 2.34 Q55749 sll0378 Uroporphyrinogen-III C-methyltransferase 2.50 In both nanofilament-forming strains, proteins involved in carbon fixation were found to be the most upregulated ( Tables 1 and 2 ). These include carboxysome shell...”
CW752_RS05260 uroporphyrinogen-III C-methyltransferase from Chryseobacterium sp. PMSZPI
44% identity, 54% coverage
Caur_2564 uroporphyrin-III C-methyltransferase from Chloroflexus aurantiacus J-10-fl
46% identity, 53% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...(Caur_1198), cobQ / cbiP (Caur_2560), N -transferase (Caur_2561), cobP / cobU (Caur_2562), cobB/cbiA (Caur_2563), cobA (Caur_2564, Caur_0687), cobJ (Caur_2565), cobM / cbiF (Caur_2566), cobI/cbiL (Caur_2567), cobL / cbiET (Caur_2568), cbiD (Caur_2569), cobH/cbiCH (Caur_2570), cbiK (Caur_2572), cobD / cbiB (Caur_2573), cbiMNQO (Caur_2574 to Caur_2577), cobU / cobT...”
AM1_4366 uroporphyrin-III C-methyltransferase from Acaryochloris marina MBIC11017
44% identity, 52% coverage
- The Complex Transcriptional Response of Acaryochloris marina to Different Oxygen Levels
Hernández-Prieto, G3 (Bethesda, Md.) 2017 - “...AM1_1445 , and ChlB, AM1_1539 ) were significantly reduced under microoxic conditions. Similarly, the gene AM1_4366 , encoding the uroporphyrin-III C-methyltransferase (cysG) at the branching point for the B12 (cobalamin) synthetic pathway, was significantly reduced under microoxic conditions. Another group of genes ( AM1_1222 , AM1_1223...”
- “...D-POR, ChlL 1657 421 3533 1.97 1.09 AM1_1539 D-POR, ChlB 1659 417 2684 1.99 0.69 AM1_4366 Uroporphyrin-III C-methyltransferase, CysG 195 92 430 1.08 1.14 AM1_2801 Protein with homology to HemJ 182 178 162 0.03 0.17 AM1_0467 O 2 -independent coproporphyrinogen III oxidase, HemN 6 822 5...”
MTBMA_c06180 uroporphyrinogen-III C-methyltransferase from Methanothermobacter marburgensis str. Marburg
44% identity, 52% coverage
MTH167 S-adenosyl-L-methionine uroporphyrinogen methyltransferase from Methanothermobacter thermautotrophicus str. Delta H
45% identity, 50% coverage
Q72M22 uroporphyrinogen-III C-methyltransferase from Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)
40% identity, 51% coverage
PA1778 uroporphyrin-III C-methyltransferase from Pseudomonas aeruginosa PAO1
NP_250469 uroporphyrin-III C-methyltransferase from Pseudomonas aeruginosa PAO1
46% identity, 51% coverage
- A High-Throughput Method for Identifying Novel Genes That Influence Metabolic Pathways Reveals New Iron and Heme Regulation in Pseudomonas aeruginosa
Glanville, mSystems 2021 - “...nirE PA0510 Uroporphyrin III c -methyltransferase 1 154 Siroheme cysG PA2611 Siroheme synthase 155 cobA PA1778 Uroporphyrin III methyltransferase 1 155 Colbamin (B12) cobI PA2904 Cobalt-factor-2 C20-methyltransferase 156 cobG PA2906 Precorrin-3B synthase 1 156 cobJ PA2903 Precorrin-3B C17-methyltransferase 156 cobM PA2948 Cobalt-precorrin-4 C11-methyltransferase 156 cobF Precorrin-6A...”
- To Hit or Not to Hit, That Is the Question - Genome-wide Structure-Based Druggability Predictions for Pseudomonas aeruginosa Proteins
Sarkar, PloS one 2015 - “.../ ANP 9 26.8528.66 9 / 0 / 0 Serine-threonine kinase Stk1 Stk1 195 (42) PA1778 1cbf / SAH 9 28.6546.15 9 / 0 / 0 Uroporphyrin-III C-methyltrans-ferase cobA - PA2086 1vj5 / CIU 7 29.8229.82 7 / 0 / 0 Probable epoxide hydrolase 336 (62)...”
- Complete genome sequence of the biocontrol strain Pseudomonas protegens Cab57 discovered in Japan reveals strain-specific diversity of this species
Takeuchi, PloS one 2014 - “...(Cluster S2: Fig. 5 and Table S5 ). In P. aeruginosa PAO1, the corresponding locus PA1778 to PA1786 was inferred to encode functions required for a nitrite/nitrate assimilation operon, due to homologies with genes reported in other bacteria, and its function has already been demonstrated [35]...”
- Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa
Wang, BMC genomics 2013 - “...2.42 Rhamnosyltransferase 2 PA1671 stk1 2.78 2.99 Serine-threonine kinase PA1707 pcrH 2.09 2.23 Regulatory protein PA1778 cobA 1.92 2.14 Methyltransferase PA2193 hcnA 3.86 5.95 Hydrogen cyanide synthase PA2300 chiC 5.52 2.27 Chitinase PA2303 ambD 0.92 1.53 Taurine catabolism dioxygenase PA1899 phzA2 12.66 11.33 Phenazine biosynthesis protein...”
- A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...Pyridoxine metabolism ThrC (PA3735) Riboflavin metabolism RibD (PA4056) RibA (PA4047) RibB (PA4054) Tetrapyrrole biosynthesis CobA (PA1778) Thiamine metabolism ThiL (PA4051) Threonine and lysine metabolism LysC (PA0904) Asd (PA3117) Hom (PA3736) Ubiquinone biosynthesis UbiG (PA3171) Vitamin B6 metabolism PdxA (PA0593) These genes were sorted based on available...”
- Microarray analysis and functional characterization of the nitrosative stress response in nonmucoid and mucoid Pseudomonas aeruginosa
Firoved, Journal of bacteriology 2004 - “...PA2664 PA3877 PA3915 PA0525 PA0523 PA0524 PA1671 PA4225 PA1778 PA0024 PA4235 PA2532 PA3394 PA0291 PA3392 PA3746 PA4260 PA3549 PA1077 PA4687 PA3989 PA1796 PA3396...”
- Transcriptional regulation of nitrate assimilation in Pseudomonas aeruginosa occurs via transcriptional antitermination within the nirBD-PA1779-cobA operon.
Romeo, Microbiology (Reading, England) 2012 (PubMed)- GeneRIF: Transcriptional regulation of nitrate assimilation occurs via transcriptional antitermination within the nirBD-PA1779-cobA operon.
PA14_41563 uroporphyrin-III C-methyltransferase from Pseudomonas aeruginosa UCBPP-PA14
46% identity, 51% coverage
- Pseudomonas aeruginosa Genomic Structure and Diversity
Klockgether, Frontiers in microbiology 2011 - “...Hypothetical protein 3670384 A G PA14_41150 syn. Putative permease of ABC transporter 3711749 T C PA14_41563 syn. cobA Uroporphyrin-III C-methyltransferase 3711791 G C PA14_41563 V L cobA Uroporphyrin-III C-methyltransferase 3764383 A G PA14_42220 I M Membrane sensor domain-containing protein 3769180 C G PA14_42250 syn. pscL Type...”
Rv2847c POSSIBLE MULTIFUNCTIONAL ENZYME SIROHEME SYNTHASE CYSG: UROPORPHYRIN-III C-METHYLTRANSFERASE (UROGEN III METHYLASE) (SUMT) (UROPORPHYRINOGEN III METHYLASE) (UROM) + PRECORRIN-2 OXIDASE + FERROCHELATASE from Mycobacterium tuberculosis H37Rv
34% identity, 92% coverage
- Comparative Transcriptomics Reveal Differential Expression of Coding and Non-Coding RNAs in Clinical Strains of <i>Mycobacterium tuberculosis</i>
Mvubu, International journal of molecular sciences 2024 - “...B). Transcript 85 had no significant mRNA target while Transcript 64 and Transcript 433 targeted Rv2847c and Rv1296 , respectively, which are both involved in intermediary metabolism and respiration ( Table 4 ). The Beijing strain most expressed Transcript 354 had 130 significant mRNA targets with...”
- “...No mRNA targets - - - - smRNA Seq Transcript 64 104.24 kcal/mol cysG ( Rv2847c ) Total = 4 RPKM = 28 Intermediary metabolism and respiration 2.7 e 04 0.67 46.38 Total Seq Transcript 433_7H9 224.81 kcal/mol thrB ( Rv1296 ) Total = 165 RPKM...”
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...AWC07_17805 cbiX Sirohydrochlorin ferrochelatase Rv0259c A3N95_07805 AWC07_14695 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase Rv2847c MAB_3143c MCNS_15910 AWC07_16325 cysH Phosphoadenylyl-sulfate reductase Rv2392 MAB_1661c MCNS_35620 AWC07_21315 gltX Glutamyl-tRNA synthetase Rv2992c MAB_3298c AWC07_23235 hemA Glutamyl-tRNA reductase Rv0509 MAB_3993c MCNS_03960 AWC07_11800 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase Rv0512...”
- “...Rv0259c 12.73 1.498465882 0 0 0 0 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase Rv2847c 68.92 5.318890862 36.77 10.84717782 63.26 89.46314996 cysH Phosphoadenylyl-sulfate reductase Rv2392 312.9333333 19.43125032 503.0566667 166.2576146 0 0 gltX Glutamyl-tRNA synthetase Rv2992c 170.96 11.21727537 89.43333333 42.90810128 107.7533333 76.94248949 hemA Glutamyl-tRNA reductase Rv0509...”
- Implementation of homology based and non-homology based computational methods for the identification and annotation of orphan enzymes: using Mycobacterium tuberculosis H37Rv as a case study
Sinha, BMC bioinformatics 2020 - “...5.4.99.18 (5-(carboxyamino)imidazole ribonucleotide mutase Rv3275c 5.4.99.61(Precorrin isomerase/porphyrin and chlorophyll metabolism) 6.3.5.9 (Hydrogenobyrinic acid a,c-diamide synthase) Rv2847c 2.1.1.132 (Precorrin-6 methyltransferase) Rv2072c 6.2.1.28 (DHCA-CoA ligase) 1.2.1.3 (Aldehyde dehydrogenase (NAD(+)) Rv0458 2.3.1.16 (Acyl CoA C-acyltransferase) 6.3.3.1 (Phosphoribosylformylglycinamidine cyclo-ligase/purine metabolism) 6.3.4.18 (5-(carboxyamino)imidazole ribonucleotide synthase) Rv3276c 6.3.5.3 (Phosphoribosylformylglycinamidine synthase) Rv0803 The...”
- “...function 1.14.13.83 (Precorrin-3B synthase) Rv2064 1 4Fe-4S cluster binding/metal ion binding/oxidoreductase activity 2.1.1.152 (Precorrin-6A synthase) Rv2847c 2 Precorrin 2 dehydrogenase activity 1.3.3.3 (Coprogen oxidase/ Porphyrin and chlorophyll metabolism) Rv2388c 1 Coproporphyrinogen III oxidase 6.2.1.28 (3-alpha,7-alpha-dihydroxy-5-beta-cholestanateCoA ligase) Rv0459 1 Uncharacterized protein 6.3.3.1 Phosphoribosylformylglycinamidine cyclo-ligase Rv0809 1 Phosphoribosylaminoimidazole...”
- Purifying Selective Pressure Suggests the Functionality of a Vitamin B12 Biosynthesis Pathway in a Global Population of Mycobacterium tuberculosis
Minias, Genome biology and evolution 2018 - “...cobB Rv2848c 1,374 1.97 0.71 7 6 che1 Rv2393 846 3.90 0.71 2 2 cysG Rv2847c 1,218 2.55 0.66 4 3 cobC Rv2231c 1,095 3.56 0.63 3 3 cobQ1 Rv0255c 1,485 3.43 0.62 4 4 cobM Rv2071c 756 3.44 0.61 7 7 Rv2067c 1,224 3.02 0.61...”
- Mapping and characterization of G-quadruplexes in Mycobacterium tuberculosis gene promoter regions
Perrone, Scientific reports 2017 - “...4 GGG CGACCC GA GG CATAC GGGG CGCT GG CAT GGG CCGCC GG TATGGT GGG Rv2847c cysG 30 GGG GACC GGG CGCCGC GGG TCGCCACCATCA GGG Rv0896 gltA2 23 GGG ATGACCCGCCTGCCC GGGG T GGGG TCTCT GG CACCAT GGG Rv2367c 34 GGG TC GGGG CTGAATC GGG C GG...”
- Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry
Kelkar, Molecular & cellular proteomics : MCP 2011 - “...in the case of eight proteins (Rv2175c, Rv2847c, Rv3133c, Rv1683, Rv1612, Rv3131, Rv2986c, and Rv3001c) we found peptide evidence indicating translation...”
SMb20987 putative siroheme synthetase protein from Sinorhizobium meliloti 1021
38% identity, 71% coverage
- The Nitrate Assimilatory Pathway in Sinorhizobium meliloti: Contribution to NO Production
Ruiz, Frontiers in microbiology 2019 - “...B. japonicum the genes that encode NasC and NirA are located at different loci. cysG (SMb20987) encodes a putative uroporphyrin-III C-methyltransferase involved in the synthesis of sirohaem, the nitrite reductase cofactor. In this work we analyzed the expression profiles of narB and nirB genes in free-living...”
- “...(encoding a small nitrite reductase subunit); green, SMb20436 (encoding the putative nitrite transporter); red, cysG (SMb20987) (encoding the putative protein involved in the synthesis of sirohaem, the nitrite reductase cofactor); purple, flp . Closest homologs (protein identity) found in other rhizobia are shown with the same...”
- Absence of functional TolC protein causes increased stress response gene expression in Sinorhizobium meliloti
Santos, BMC microbiology 2010 - “...-22.7 SMb20985 nirD nitrite reductase NAD(P)H -26.6 SMb20986 narB putative nitrate reductase, large subunit -14.1 SMb20987 Putative uroporphiryn-III C-methyltransferase -7.6 SMb21094 argH2 argininosuccinate lyase -20.7 SMb21163 hutU urocanate hydratase (urocanase) -10.3 SMb21164 hutG Putative formiminoglutamase -11.5 SMb21165 hutH Putative histidine ammonia-lyase histidase -7.7 SMc01041 dusB tRNA-dihydrouridine...”
MCNS_15910 uroporphyrinogen-III C-methyltransferase from Mycobacterium conspicuum
34% identity, 95% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...H/cobC Rv2228c cobF Precorrin-6A synthase MCNS_43990 AWC07_18155 H0P51_RS23435 EET03_RS22025 cobA Probable cob(I)alamin adenosyltransferase CobO Rv2849c MCNS_15910 XA26_25280 AWC07_16325 H0P51_RS10520 EET03_RS08695 cobB Cobyrinic acid A,C-diamide synthase Rv2848c MAB_3155c MCNS_15900 AWC07_16320 cobC L-threonine 3-O-phosphate decarboxylase Rv2231c MAB_1902 cobD Adenosylcobinamide-phosphate synthase Rv2236c MAB_1898 MCNS_32370 AWC07_23980 cobG Precorrin-3B synthase Rv2064...”
- “...Sirohydrochlorin ferrochelatase Rv0259c A3N95_07805 AWC07_14695 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase Rv2847c MAB_3143c MCNS_15910 AWC07_16325 cysH Phosphoadenylyl-sulfate reductase Rv2392 MAB_1661c MCNS_35620 AWC07_21315 gltX Glutamyl-tRNA synthetase Rv2992c MAB_3298c AWC07_23235 hemA Glutamyl-tRNA reductase Rv0509 MAB_3993c MCNS_03960 AWC07_11800 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase Rv0512 MAB_3990c MCNS_03990...”
Psyr_2098 Uroporphyrin-III C-methyltransferase, C-terminal from Pseudomonas syringae pv. syringae B728a
45% identity, 52% coverage
Pden_2488 uroporphyrin-III C-methyltransferase from Paracoccus denitrificans PD1222
48% identity, 52% coverage
Q51720 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Propionibacterium freudenreichii (see paper)
cobA / AAA78897.1 uroporphyrinogen III methyltransferase from Propionibacterium freudenreichii subsp. shermanii (see 2 papers)
RM25_RS02055 uroporphyrinogen-III C-methyltransferase from Propionibacterium freudenreichii subsp. freudenreichii
45% identity, 49% coverage
MKAN_RS23630 uroporphyrinogen-III C-methyltransferase from Mycobacterium kansasii ATCC 12478
33% identity, 92% coverage
SYNPCC7002_A1192 uroporphyrin-III synthase/methyltransferase from Synechococcus sp. PCC 7002
44% identity, 48% coverage
AWC07_16325 uroporphyrinogen-III C-methyltransferase from Mycobacterium gastri
33% identity, 92% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...cobF Precorrin-6A synthase MCNS_43990 AWC07_18155 H0P51_RS23435 EET03_RS22025 cobA Probable cob(I)alamin adenosyltransferase CobO Rv2849c MCNS_15910 XA26_25280 AWC07_16325 H0P51_RS10520 EET03_RS08695 cobB Cobyrinic acid A,C-diamide synthase Rv2848c MAB_3155c MCNS_15900 AWC07_16320 cobC L-threonine 3-O-phosphate decarboxylase Rv2231c MAB_1902 cobD Adenosylcobinamide-phosphate synthase Rv2236c MAB_1898 MCNS_32370 AWC07_23980 cobG Precorrin-3B synthase Rv2064 MAB_2200c MCNS_30550...”
- “...ferrochelatase Rv0259c A3N95_07805 AWC07_14695 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase Rv2847c MAB_3143c MCNS_15910 AWC07_16325 cysH Phosphoadenylyl-sulfate reductase Rv2392 MAB_1661c MCNS_35620 AWC07_21315 gltX Glutamyl-tRNA synthetase Rv2992c MAB_3298c AWC07_23235 hemA Glutamyl-tRNA reductase Rv0509 MAB_3993c MCNS_03960 AWC07_11800 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase Rv0512 MAB_3990c MCNS_03990 XA26_52000...”
BLBBGE_278 uroporphyrinogen-III C-methyltransferase from Blattabacterium sp. (Blattella germanica) str. Bge
41% identity, 53% coverage
I35_RS08110 uroporphyrinogen-III C-methyltransferase from Burkholderia cenocepacia H111
46% identity, 51% coverage
- Transcriptional Response of Burkholderia cenocepacia H111 to Severe Zinc Starvation
Barnett, British journal of biomedical science 2023 - “...2.107024951 0.005429617 I35_RS00155 ATP synthase FoF1 subunit A 2.183650426 0.013504754 I35_RS07930 Peptidase M23 2.283848351 0.027058355 I35_RS08110 Uroporphyrin-III C-methyltransferase 2.348103878 0.0000143 I35_RS13745 MIP family channel protein 2.34927356 0.015213303 I35_RS04075 Hypothetical protein 2.355094182 0.00130666 I35_RS10765 Glutamine synthetase 2.366860655 0.01636581 I35_RS00165 ATP synthase subunit B 2.411303669 0.003024043 I35_RS14515 Dioxygenase...”
ZMO1271 uroporphyrin-III C-methyltransferase from Zymomonas mobilis subsp. mobilis ZM4
40% identity, 50% coverage
Mchl_5731 uroporphyrin-III C-methyltransferase from Methylobacterium chloromethanicum CM4
45% identity, 54% coverage
PP_2090 uroporphyrin-III C-methyltransferase from Pseudomonas putida KT2440
44% identity, 50% coverage
corA / P29564 uroporphyrinogen-III C-methyltransferase subunit from Methanobacterium ivanovii (see paper)
SUMT_METIV / P29564 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Methanobacterium ivanovii (see paper)
P29564 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Methanobacterium ivanovii (see paper)
CORA / AAA72997.1 S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase from Methanobacterium ivanovii (see paper)
44% identity, 49% coverage
- function: Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and coenzyme F430.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459)
catalytic activity: uroporphyrinogen III + S-adenosyl-L-methionine = precorrin-1 + S-adenosyl-L-homocysteine + H(+) (RHEA:19089)
catalytic activity: precorrin-1 + S-adenosyl-L-methionine = precorrin-2 + S- adenosyl-L-homocysteine (RHEA:21972)
subunit: Homodimer.
Synpcc7942_0271 uroporphyrin-III C-methyltransferase from Synechococcus elongatus PCC 7942
42% identity, 52% coverage
EET03_RS08695 uroporphyrinogen-III C-methyltransferase from Mycobacterium innocens
32% identity, 92% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...synthase MCNS_43990 AWC07_18155 H0P51_RS23435 EET03_RS22025 cobA Probable cob(I)alamin adenosyltransferase CobO Rv2849c MCNS_15910 XA26_25280 AWC07_16325 H0P51_RS10520 EET03_RS08695 cobB Cobyrinic acid A,C-diamide synthase Rv2848c MAB_3155c MCNS_15900 AWC07_16320 cobC L-threonine 3-O-phosphate decarboxylase Rv2231c MAB_1902 cobD Adenosylcobinamide-phosphate synthase Rv2236c MAB_1898 MCNS_32370 AWC07_23980 cobG Precorrin-3B synthase Rv2064 MAB_2200c MCNS_30550 XA26_35740 AWC07_17760...”
A5717_05685 uroporphyrinogen-III C-methyltransferase from Mycolicibacterium porcinum
33% identity, 92% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...H/cobC cobF Precorrin-6A synthase MKAN_RS08645 A5717_31225 MXEN_19174 MSMEG_5548 cobA Probable cob(I)alamin adenosyltransferase CobO MKAN_RS09965 CDN37_RS24000 A5717_05685 MXEN_03569 AWC27_RS04140 cobB Cobyrinic acid A,C-diamide synthase A5717_05680 MXEN_03564 MSMEG_2617 cobC L-threonine 3-O-phosphate decarboxylase cobD Adenosylcobinamide-phosphate synthase MKAN_03275 A5717_01680 MXEN_00720 MSMEG_4310 cobG Precorrin-3B synthase MKAN_RS01775 CDN37_RS01855 MPHLCCUG_RS13200 A5717_31635 MXEN_01317 AWC27_RS14575...”
- “...cbiX Sirohydrochlorin ferrochelatase - A5717_10190 SAMEA4434518_00227 MXEN_11286 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase A5717_05685 MXEN_03559 cysH Phosphoadenylyl-sulfate reductase A5717_28590 SAMEA4434518_01414 MXEN_11291 gltX Glutamyl-tRNA synthetase A5717_14355 MXEN_16257 MSMEG_2383 hemA Glutamyl-tRNA reductase A5717_22190 SAMEA4434518_00496 MXEN_04673 MSMEG_0919 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase MKAN_RS17655 CDN37_RS17885 MPHLCCUG_RS22000 SAMEA4434518_00499...”
MSMEG_2618 uroporphyrin-III C-methyltransferase from Mycobacterium smegmatis str. MC2 155
33% identity, 92% coverage
- c-di-AMP Accumulation Regulates Growth, Metabolism, and Immunogenicity of Mycobacterium smegmatis
Ning, Frontiers in microbiology 2022 - “...c-di-AMP in M. smegmatis significantly upregulated the expression of porphyrin-metabolizing enzymes in 3-day culture, including MSMEG_2618 (uroporphyrin-III C-methyltransferase), MSMEG_0954 (uroporphyrinogen-III synthase), and MSMEG_2780 (uroporphyrinogen decarboxylase) ( Figure 6C ), and downregulated the expression of MSMEG_4525 (putative oxygen-independent coproporphyrinogen III oxidase) ( Figure 6C ), which may...”
MW2320 uroporphyrin-III C-methyl transferase from Staphylococcus aureus subsp. aureus MW2
41% identity, 52% coverage
- Nucleotide substitution and recombination at orthologous loci in Staphylococcus aureus
Hughes, Journal of bacteriology 2005 - “...MW1889, MW1895, MW1928, MW1932, MW1962, MW2132, MW2254, MW2320, MW2321, MW2396, MW2449, MW2466, MW2507, MW2551, MW2576, Protein function VOL. 187, 2005 2703...”
MAB_3143c Possible multifunctional enzyme siroheme synthase CysG/Uroporphyrin-III C-methyltransferase-like from Mycobacterium abscessus ATCC 19977
32% identity, 91% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...cbiX Sirohydrochlorin ferrochelatase Rv0259c A3N95_07805 AWC07_14695 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase Rv2847c MAB_3143c MCNS_15910 AWC07_16325 cysH Phosphoadenylyl-sulfate reductase Rv2392 MAB_1661c MCNS_35620 AWC07_21315 gltX Glutamyl-tRNA synthetase Rv2992c MAB_3298c AWC07_23235 hemA Glutamyl-tRNA reductase Rv0509 MAB_3993c MCNS_03960 AWC07_11800 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase Rv0512 MAB_3990c...”
- “...transporter permease Anaerobic pathway cbiX Sirohydrochlorin ferrochelatase Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase MAB_3143c 95.06666667 7.895709806 cysH Phosphoadenylyl-sulfate reductase MAB_1661c 174.4733333 18.36925783 gltX Glutamyl-tRNA synthetase MAB_3298c 287.6433333 11.14623853 MSMEG_2383 156.9933333 19.13254383 hemA Glutamyl-tRNA reductase MAB_3993c 268.16 8.943438936 MSMEG_0919 174.06 98.84548143 hemB Probable delta-aminolevulinic acid...”
SCO1553 uroporphyrin-III methyltransferase from Streptomyces coelicolor A3(2)
33% identity, 91% coverage
- Deciphering the Transcriptional Response Mediated by the Redox-Sensing System HbpS-SenS-SenR from Streptomycetes
Busche, PloS one 2016 - “...1.943 SCO1851 a cobO cob(I)alamin adenosyltransferase 0.64 1.6 SCO1852 a cobI precorrin-2 C20-methyltransferase 0.87 1.83 SCO1553 a cysG uroporphyrin-III C-methyltransferase 1.20 2.30 SCO1554 a cobT nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase 1.14 2.20 SCO5227 a nrdX redoxin 1.45 2.73 SCO5226 a nrdA ribonucleotide reductase large chain 1.45 2.73 SCO5225 a...”
- “...in synthesis of the corrin ring of cobalamin (SCO1847-SCO1853) ( S1 Fig ), uroporphyrin-III methyltransferase (SCO1553, named cysG ) and nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltranferase (SCO1554, named cobT ). CysG is predicted to catalyse the S-adenosylmethionine-dependent methylation of uroporphyrinogen III, leading to the product sirohydrochlorin that is the common...”
- Genome-scale analysis reveals a role for NdgR in the thiol oxidative stress response in Streptomyces coelicolor
Kim, BMC genomics 2015 - “...Name Function Category a Note b SCO1552 rRNA methylase 2.2.11 RNA synthesis, modification, DNA transcript'n SCO1553 cysG Putative uroporphyrin-III methyltransferase 3.2.6 Heme, porphyrin * SCO1707 Putative ABC sugar transporter, ATP-binding subunit 1.5.0 Transport/binding proteins * SCO1776 pyrG Putative CTP synthetase 3.3.11 Nucleotide interconversions *, I SCO2103...”
- “...]. Moreover, NdgR bound to the promoter region of putative siroheme synthase encoded by cysG (SCO1553). Siroheme is a prosthetic group that participates in six-electron reduction reactions catalyzed by both sulfite and nitrite reductases. CysG converts uroporphyrinogen III, which is a precursor of heme and cobalamin...”
AO090020000339 No description from Aspergillus oryzae RIB40
27% identity, 79% coverage
A0A0H3KAA0 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Staphylococcus aureus (see paper)
NWMN_RS13215, SAUSA300_RS12940 uroporphyrinogen-III C-methyltransferase from Staphylococcus aureus subsp. aureus str. Newman
SAUSA300_2344 uroporphyrin-III C-methyl transferase from Staphylococcus aureus subsp. aureus USA300_FPR3757
SACOL2396 uroporphyrinogen III methylase SirB, putative from Staphylococcus aureus subsp. aureus COL
41% identity, 51% coverage
- Global Transcriptomic Analysis of Bacteriophage-Host Interactions between a Kayvirus Therapeutic Phage and Staphylococcus aureus
Finstrlová, Microbiology spectrum 2022 - “...NarH 6.04 5.60 n.s. n.s. NWMN_RS13210 Nitrate reductase subunit alpha NarG 6.64 6.26 n.s. n.s. NWMN_RS13215 Uroporphyrinogen-III C-methyltransferase CobA 6.99 5.80 n.s. n.s. NWMN_RS13220 Nitrite reductase small subunit NirD 9.74 4.93 n.s. n.s. NWMN_RS13225 NAD(P)/FAD-dependent oxidoreductase NasD 26.63 13.53 n.s. n.s. NWMN_RS13230 Sirohydrochlorin chelatase SirB 22.27...”
- Network-based genetic investigation of virulence-associated phenotypes in methicillin-resistant Staphylococcus aureus
Kim, Scientific reports 2018 - “...Ability (Significance) 1 SAUSA300_RS12890 1.73569E-08 FALSE Not tested 2 SAUSA300_RS12950 9.84818E-08 TRUE (1) FALSE 3 SAUSA300_RS12940 1.04527E-05 TRUE (2) FALSE 4 SAUSA300_RS12935 3.05251E-05 FALSE Not tested 5 SAUSA300_RS03540 3.89378E-05 FALSE Not tested 6 SAUSA300_RS04955 5.47043E-05 FALSE Not tested 7 SAUSA300_RS12930 0.000143066 FALSE Not tested 8 SAUSA300_RS13325...”
- VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus
Boyle-Vavra, Antimicrobial agents and chemotherapy 2013 - “...4.6 Nitrate/nitrite metabolism Nitrate/nitrite metabolism SAUSA300_2342 2.7 SAUSA300_2344 SA2186 This study 3.6 6.8 5.5 SA2187 This study 6.3 4 SAUSA300_2346...”
- Protein CoAlation and antioxidant function of coenzyme A in prokaryotic cells
Tsuchiya, The Biochemical journal 2018 - “...32.773 QETTEWC*EK 218.02 SAOUHSC_02146 Uncharacterized protein 40.354 ESGC*TVFQGK 93.345 SAOUHSC_02146 Uncharacterized protein 40.354 LILENC*R 125.68 SACOL2396 Uroporphyrinogen III methylase SirB, putative 36.29 INDC*IVEAAR 113.37 glpK Glycerol kinase 55.625 ATLESLC*YQTR 158.91 glpK Glycerol kinase 55.625 QTQSIC*SELKQQGYEQTFR 125.84 HMPREF3211_00337 Methyltransferase domain protein 21.763 ALDIGC*GSGLLVEK 55.031 tarJ Ribulose-5-phosphate reductase...”
- Staphylococcus aureus gene expression in a rat model of infective endocarditis
Hanses, Genome medicine 2014 - “...pflB SACOL0204 44.7 46.9 59.9 65.6 formate acetyltransferase rbsK SACOL0253 6.9 2.5 4.9 ns. ribokinase SACOL2396 19.0 14.3 7.4 6.1 uroporphyrin-III C-methyl transferase SACOL1476 24.8 22.4 18.7 18.6 amino acid permease Regulators agrA SACOL2026 ns ns 4.8 ns accessory gene regulator protein A agrC SACOL2025 -2.6...”
- Changes in the Staphylococcus aureus transcriptome during early adaptation to the lung
Chaffin, PloS one 2012 - “...SACOL0491 were upregulated in vivo. The uroporphyrin-III C-methyltransferase gene sirB ( nasF / cobA , SACOL2396), involved in siroheme synthesis, was 3.0 to 3.6 fold higher after 30 in vivo than in either of the in vitro conditions. Siroheme is a co-factor of nitrite reductase, which...”
- Rbf promotes biofilm formation by Staphylococcus aureus via repression of icaR, a negative regulator of icaADBC
Cue, Journal of bacteriology 2009 - “...SACOL2070 SACOL2397 SACOL0696 SACOL0569 SACOL1591 SACOL2138 SACOL2357 SACOL2386 SACOL2396 N315-A1617 SACOL0568 SACOL1790 5.4 2.1 2.2 2.2 2.1 2.1 2.3 2.1 2.1 2.0...”
SPO2632 uroporphyrinogen-III C-methyltransferase from Ruegeria pomeroyi DSS-3
44% identity, 50% coverage
- Transcriptional changes underlying elemental stoichiometry shifts in a marine heterotrophic bacterium
Chan, Frontiers in microbiology 2012 - “...polymerase factor sigma-32 ( rpoH-2 ) 12.5 SPO2596 5-amino-levulinate synthase ( hemA-1 ) 1.8 4.6 SPO2632 Uroporphyrinogen-III C -methyltransferase ( cobA-1 ) 3.4 5.9 SPO2634 Sulfite reductase, putative 5.7 5.3 SPO3383 Thiol-specific antioxidant protein 4.9 4.5 SPO3527 Universal stress protein family protein 3.7 5.3 SPO3532 Coproporphyrinogen...”
- “...4.0 3.5 SPO1409 rpoH-2 RNA polymerase factor sigma-32 12.5 SPO2596 hemA-1 5-amino-levulinate synthase 1.8 4.6 SPO2632 cobA-1 Uroporphyrin-III C -methyltransferase 3.4 5.9 SPO2634 Sulfite reductase, putative 5.7 5.3 SPO3383 Thiol-specific antioxidant protein 4.9 4.5 SPO3527 Universal stress protein family protein 3.7 5.3 SPO3532 hemN Coproporphyrinogen III...”
Caur_0687 uroporphyrin-III C-methyltransferase from Chloroflexus aurantiacus J-10-fl
43% identity, 51% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...cobQ / cbiP (Caur_2560), N -transferase (Caur_2561), cobP / cobU (Caur_2562), cobB/cbiA (Caur_2563), cobA (Caur_2564, Caur_0687), cobJ (Caur_2565), cobM / cbiF (Caur_2566), cobI/cbiL (Caur_2567), cobL / cbiET (Caur_2568), cbiD (Caur_2569), cobH/cbiCH (Caur_2570), cbiK (Caur_2572), cobD / cbiB (Caur_2573), cbiMNQO (Caur_2574 to Caur_2577), cobU / cobT (Caur_2578),...”
XAC2159 siroheme synthase from Xanthomonas axonopodis pv. citri str. 306
41% identity, 71% coverage
- The sulfur/sulfonates transport systems in Xanthomonas citri pv. citri
Pereira, BMC genomics 2015 - “...CysG from S. typhimurium. (A) Positioning of the cysG genes in the X. citri genome. Xac2159 and Xac2157 genes, encoding respectively, an uroporphyrin-III C-methyl transferase and a syroheme synthase, are closely located in opposite directions. The third gene, Xac3341, encoding another syroheme synthase is located in...”
SAOUHSC_02682 uroporphyrin-III C-methyltransferase, putative from Staphylococcus aureus subsp. aureus NCTC 8325
41% identity, 51% coverage
MXEN_03559 uroporphyrinogen-III C-methyltransferase from Mycobacterium xenopi RIVM700367
33% identity, 92% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Sirohydrochlorin ferrochelatase - A5717_10190 SAMEA4434518_00227 MXEN_11286 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase A5717_05685 MXEN_03559 cysH Phosphoadenylyl-sulfate reductase A5717_28590 SAMEA4434518_01414 MXEN_11291 gltX Glutamyl-tRNA synthetase A5717_14355 MXEN_16257 MSMEG_2383 hemA Glutamyl-tRNA reductase A5717_22190 SAMEA4434518_00496 MXEN_04673 MSMEG_0919 hemB Probable delta-aminolevulinic acid dehydratase/porphobilinogen synthase MKAN_RS17655 CDN37_RS17885 MPHLCCUG_RS22000 SAMEA4434518_00499 AWC27_RS21685...”
SAR2487 tetrapyrrole (corrin/porphyrin) methylase family protein from Staphylococcus aureus subsp. aureus MRSA252
40% identity, 52% coverage
SA2186 uroporphyrin-III C-methyl transferase from Staphylococcus aureus subsp. aureus N315
39% identity, 54% coverage
- VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus
Boyle-Vavra, Antimicrobial agents and chemotherapy 2013 - “...Nitrate/nitrite metabolism Nitrate/nitrite metabolism SAUSA300_2342 2.7 SAUSA300_2344 SA2186 This study 3.6 6.8 5.5 SA2187 This study 6.3 4 SAUSA300_2346 SA2188...”
- Serine/threonine phosphatase Stp1 contributes to reduced susceptibility to vancomycin and virulence in Staphylococcus aureus
Cameron, The Journal of infectious diseases 2012 - “...Transglycosylase 1.86 2.37 yfbB Acyl-CoA thioester hydrolase SA2186 nasF Uroporphyrin-III C-methyl transferase 2.16 SA1082 SA1184 rimM citB 16S ribosomal RNA...”
- Characterization of the oxygen-responsive NreABC regulon of Staphylococcus aureus
Schlag, Journal of bacteriology 2008 - “...Materials and Methods. The genes nirR (SA2189) and nasF (SA2186) are likely to be involved in the synthesis of siroheme, the cofactor of the nitrite reductase...”
- “...SA2085 SA2086 SA2149 SA2150 SA2176 SA2183 SA2184 SA2185 SA2186 SA2187 SA2188 SA2189 Product 7852 SCHLAG ET AL. J. BACTERIOL. TABLE 2. Genes differentially...”
- Characterization of the Staphylococcus aureus heat shock, cold shock, stringent, and SOS responses and their effects on log-phase mRNA turnover
Anderson, Journal of bacteriology 2006 - “...SA2180 SA2183 SA2184 SA2185 SA2186 SA2628 6-Phospho-beta-glucosidase Alpha-glucosidase 6-Phospho-beta-galactosidase Tagatose 1,6-diphosphate aldolase...”
- Nucleotide substitution and recombination at orthologous loci in Staphylococcus aureus
Hughes, Journal of bacteriology 2005 - “...SA1761 SA1765 SA1799 SA1801 SA1843 SA2008 SA2125 SA2186 SA2187 SA2260 SA2317 SA2333 SA2373 SA2423 SA2448 Unknown Unknown Cassette chromosome recombinase B...”
- Multidrug resistance in Staphylococcus aureus due to overexpression of a novel multidrug and toxin extrusion (MATE) transport protein
Kaatz, Antimicrobial agents and chemotherapy 2005 - “...Berkeley SA0095 SA0404 SA0405 SA0406 SA0757 SA1321 SA2184 SA2185 SA2186 SA2549 Gene VOL. 49, 2005 NOVEL S. AUREUS MATE MDR TRANSPORTER 1861 The positioning of...”
- Role of a cysteine synthase in Staphylococcus aureus
Lithgow, Journal of bacteriology 2004 - “...amino acid identity); CysJ (SA2413, 50% identity); YlnD (SA2186, 42% identity); YlnE (SA2189, 21% identity); YlnF (SA2186, 35% identity); and the -glutamyl...”
- Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci
Dunman, Journal of bacteriology 2001 - “...ND 3.7 SA2187 SA2312 SA0433 SA0915 SA1491 SA2186 SA0537 Amino acid metabolism Amino acid metabolism Carbohydrate metabolism Carbohydrate metabolism Coenzyme...”
DRA0011 uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase from Deinococcus radiodurans R1
49% identity, 46% coverage
AFUA_3G06600 siroheme synthase, putative from Aspergillus fumigatus Af293
28% identity, 76% coverage
VF_1531 ferrochelatase from Vibrio fischeri ES114
VF_1531 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Aliivibrio fischeri ES114
36% identity, 61% coverage
- Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes
Wasilko, Molecular microbiology 2021 - “...sulfate as the sole sulfur source ( Table 2 ). Transposon-insertion mutants for cobA and VF_1531 also failed to grow on sulfate alone ( Table 2 ), which is consistent with their predicted roles in the synthesis of the essential siroheme cofactor of CysIJ sulfite reductase...”
- “...cysG gene encodes siroheme synthase and its N-and C-termini exhibit significant identity with cobA and VF_1531 , respectively ( Table 1 ). The yfcA gene, which is predicted to encode an inner membrane protein, was also hit in the screen multiple times ( Table 2 ),...”
SERP_RS09915 uroporphyrinogen-III C-methyltransferase from Staphylococcus epidermidis RP62A
SERP1988 uroporphyrinogen-III methylase SirB, putative from Staphylococcus epidermidis RP62A
40% identity, 50% coverage
XA26_25280 uroporphyrinogen-III C-methyltransferase from Mycolicibacterium fortuitum
31% identity, 92% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Rv2228c cobF Precorrin-6A synthase MCNS_43990 AWC07_18155 H0P51_RS23435 EET03_RS22025 cobA Probable cob(I)alamin adenosyltransferase CobO Rv2849c MCNS_15910 XA26_25280 AWC07_16325 H0P51_RS10520 EET03_RS08695 cobB Cobyrinic acid A,C-diamide synthase Rv2848c MAB_3155c MCNS_15900 AWC07_16320 cobC L-threonine 3-O-phosphate decarboxylase Rv2231c MAB_1902 cobD Adenosylcobinamide-phosphate synthase Rv2236c MAB_1898 MCNS_32370 AWC07_23980 cobG Precorrin-3B synthase Rv2064 MAB_2200c...”
CC0618 siroheme synthase from Caulobacter crescentus CB15
42% identity, 50% coverage
An11g09700 uncharacterized protein from Aspergillus niger
28% identity, 79% coverage
RSP_1944 Uroporphiryn-III C-methyltransferase/siroheme synthase from Rhodobacter sphaeroides 2.4.1
41% identity, 50% coverage
sll0166 uroporphyrin-III synthase from Synechocystis sp. PCC 6803
37% identity, 48% coverage
TTHB060 S-adenosyl-L-methionine uroporphyrinogen methyltransferase from Thermus thermophilus HB8
44% identity, 51% coverage
- Complete Genome Sequence of Thermus aquaticus Y51MC23
Brumm, PloS one 2015 - “...eleven gene cluster (genes 976 through 986) that is syntenous to pTT27 genes TTHB051 through TTHB060. Remaining orthologs to the pTT27 pathway are scattered through the Y51MC23 chromosome. Y51MC23 encodes genes for the pathways involved in biosynthesis of riboflavin, nicotinate/NAD, and folate. Genes coding for pathways...”
FOIG_05965 uroporphyrin-III C-methyltransferase/precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Fusarium odoratissimum NRRL 54006
26% identity, 77% coverage
I35_RS12715 uroporphyrinogen-III C-methyltransferase from Burkholderia cenocepacia H111
40% identity, 52% coverage
- Transcriptional Response of Burkholderia cenocepacia H111 to Severe Zinc Starvation
Barnett, British journal of biomedical science 2023 - “...2.411303669 0.003024043 I35_RS14515 Dioxygenase 2.415249229 0.000261085 I35_RS32500 Spermidine synthase 2.433711956 0.001790561 I35_RS03095 Thioredoxin 2.460179293 0.027058355 I35_RS12715 Uroporphyrin-III C-methyltransferase 2.527603678 0.003439743 I35_RS16655 n-acetyl-gamma-glutamyl-phosphate reductase 2.686444773 0.001583599 I35_RS22845 Lysine transporter LysE 2.724901267 0.000529129 I35_RS10190 RNA helicase 3.068648173 0.025920137 I35_RS32515 Hypothetical protein 3.300579506 1.14E08 I35_RS32510 Aldolase 3.393813782 0.00000587 I35_RS32505...”
BCAM1687 putative uroporphyrin-III C-methyltransferase from Burkholderia cenocepacia J2315
44% identity, 51% coverage
B7G0M8 Tetrapyrrole methylase domain-containing protein (Fragment) from Phaeodactylum tricornutum (strain CCAP 1055/1)
38% identity, 50% coverage
XP_001742170 uncharacterized protein from Monosiga brevicollis MX1
41% identity, 55% coverage
- Cyanobacterial contribution to the genomes of the plastid-lacking protists
Maruyama, BMC evolutionary biology 2009 - “...gruberi D. discoideum S. cerevisiae M. brevicollis Uroporphyrin III methyltransferase porphyrin 51635 - - - XP_001742170 Cobalamin-independent methionine synthase methionine 72019 - - - - Amino acid decarboxylase amino acid - 36109 - - - TIC55-like oxidoreductase unknown - 52597 - - - Folate/biopterin transporter folate...”
SPCC1739.06c uroporphyrin methyltransferase (predicted) from Schizosaccharomyces pombe
29% identity, 72% coverage
- Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast
Guo, G3 (Bethesda, Md.) 2016 - “...(5135 genes) Genes Biological process Sulfur compound metabolic process 15 3.40e07 27.78% (10) 1.81% (93) SPCC1739.06c , cys2 , gcs2 , gsa1 , met14 , cys11 , hmt2 , sua1 , met16 , met8 Sulfur amino acid metabolic process 25 3.11e05 16.67% (6) 0.66% (34) SPCC1739.06c...”
- “..., gsa1 , hmt2 Cofactor biosynthetic process 12 5.30e5 22.22% (8) 1.85% (95) snz1 , SPCC1739.06c , coq3 , nde2 , coq5 , dps1 , coq4 , coq9 , met8 Ubiquinone biosynthetic process 56 6.30e06 13.89% (5) 0.25% (13) coq9 , coq4 , coq3 , coq5...”
- A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe
Kennedy, Toxicological sciences : an official journal of the Society of Toxicology 2008 - “...SPCC737.09c None None met14 sua1 SPCC1739.06c SPAC1782.11 SPBC27.08c SPBC1198.08 MET1/YKR069W MET14/YKL001C MET3/YJR010W YFR044C cys11 SPBC36.04 CYS12/YGR012W...”
- “...SPAC13G7.06/met16 SPCC584.01c/sir1 SPAC4D7.06c SPCC1739.06c SPBC1711.04 SPBC36.04/cys11 SPAC1039.08/ SPBC8D2.18c/pi047 SPAC22F3.10c/gcs1 SPBC2G5.06c/hmt2...”
- SCF(Pof1)-ubiquitin and its target Zip1 transcription factor mediate cadmium response in fission yeast
Harrison, The EMBO journal 2005 - “...name Function Budding yeast SPAC869.05c SPCC1739.06c SPCPB1C11.03 SPBPB10D8.02c SPBPB10D8.01 pof1+ SPBC106.17c SPBPB2B2.08 SPBP16F5.08c SPAC10F6.01c frp1+...”
Pden_2556 uroporphyrin-III C-methyltransferase from Paracoccus denitrificans PD1222
44% identity, 50% coverage
Tph_c15350 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Thermacetogenium phaeum DSM 12270
39% identity, 39% coverage
MET1 potential uroporphyrin-3 C-methyltransferase from Candida albicans (see 3 papers)
29% identity, 65% coverage
- CharProtDB CGD description: Putative uroporphyrin-3 C-methyltransferase; enzyme of methionine biosynthesis; upregulated in the presence of human whole blood or polymorphonuclear (PMN) cells; Hog1p-induced; fungal-specific (no human or murine homolog)
MET1 / P36150 uroporphyrinogen-III C-methyltransferase (EC 2.1.1.107) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (see 2 papers)
SUMT_YEAST / P36150 Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; EC 2.1.1.107 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
YKR069W Met1p from Saccharomyces cerevisiae
35% identity, 42% coverage
- function: Siroheme synthase involved in methionine biosynthesis.
catalytic activity: uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+) (RHEA:32459) - Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid
Lairón-Peris, Microbial genomics 2021 - “...also explored the expression of genes involved in sulfite consumption by sulfite reductase [ MET1 (YKR069W), MET5 (YJR137C), MET8 (YBR213W), MET10 (YFR030W)], and sulfitolysis of glutathione [ GLR1 (YPL091W)]. Of them, the MET5 S. cerevisiae allele was overexpressed in H14A7-etoh at the latency phase at 15...”
- The Recent De Novo Origin of Protein C-Termini
Andreatta, Genome biology and evolution 2015 - “...cer 265 3 10 Frameshift 2 Strong Strong Mitochondrial ribosomal protein of the large subunit YKR069W MET1 S. cer 591 2 2 Point 2 Low Strong Uroporphyrinogen III transmethylase activity; sulfate assimilation and methionine biosynthesis YLR095C IOC2 S. cer 816 11 38 Frameshift 2 Strong Strong...”
- Comprehensive structural and substrate specificity classification of the Saccharomyces cerevisiae methyltransferome
Wlodarski, PloS one 2011 - “...Protein YDR410C (STE14) Lipid YGR157W (CHO2), YJR073C (OPI3) Tetrapyrrole methylase Protein YLR172C (DPH5) Small molecule YKR069W (MET1) DNA/RNA-binding 3-helical bundle Protein YDL200C a (MGT1) TIM beta/alpha-barrel Small molecule YER091C b (MET6), YLL062C (MHT1), YPL273W (SAM4) Thymidylate synthetase Small molecule YOR074C c (CDC21) MTases are grouped according...”
- Identification of MET10-932 and characterization as an allele reducing hydrogen sulfide formation in wine strains of Saccharomyces cerevisiae
Linderholm, Applied and environmental microbiology 2010 - “...(Ba126) UCD950 (Ba196) UCD956 (Ba224) UCD957 (Ba229) UCD522 YKR069W BY4742 YJR137C BY4742 YBR213W BY4742 YFR030W BY4742 ALY38 ALY39 ALY95 ALY72 ALY40 ALY41...”
- Transcriptional response to mitochondrial NADH kinase deficiency in Saccharomyces cerevisiae
Stuart, Mitochondrion 2009 - “...growth YNL036W NCE103 -3.40 -2.77 -2.34 4.17 3.62 Carbonic anhydrase; poorly transcribed under aerobic conditions YKR069W MET1 -3.27 -2.23 -2.67 1.76 1.81 Sulfate assimilation, methionine metabolism, and siroheme biosynthesis YPL171C OYE3 -3.25 3.77 -1.13 1.56 -2.38 Conserved NADPH oxidoreductase; may be involved in sterol metabolism YJR010W...”
- Positional analyses of BRCA1-dependent expression in Saccharomyces cerevisiae
Skibbens, Cell cycle (Georgetown, Tex.) 2008 - “...Up 6.01615 Up YGR292W MAL12 2.286683 Up 6.6888905 Up YBR299W MAL32 3.6775527 Up 2.285604 Up YKR069W MET1 3.3087142 Up 3.3314137 Up YFR030W MET10 3.7851515 Up 3.1707487 Up YPR167C MET16 2.9498475 Up 2.9523308 Up YLR303W MET17 12.952447 Up 6.8508286 Up YNL277W MET2 4.7936215 Up 3.8631165 Up YIR017C...”
- Identification of molecular pathways affected by pterostilbene, a natural dimethylether analog of resveratrol
Pan, BMC medical genomics 2008 - “...Gene Description a. Methionine metabolism YJR137C ECM17 -2.5 2.48 10 -5 Sulfite reductase beta subunit YKR069W MET1 -3.1 3.88 10 -4 AdoMet-dependent uroporphyrinogen III transmethylase YJR010W MET3 -3.8 3.5 10 -4 ATP sulfurylase YER091C MET6 -2.4 8.83 10 -5 Cobalamin-independent methionine synthase YFR030W MET10 -2.1 1.5...”
- Identification of coherent patterns in gene expression data using an efficient biclustering algorithm and parallel coordinate visualization
Cheng, BMC bioinformatics 2008 - “...microtubule nucleation 1.13E-04 5.52E-03 YDR356W, YKL042W, YOR373W, YPL124W 57 sulfate assimilation 1.76E-04 9.50E-03 YFR030W, YJR010W, YKR069W microtubule nucleation 1.24E-04 6.67E-03 YBL063W, YMR117C, YOR373W, YPL124W 59 DNA replication checkpoint 5.86E-04 3.23E-02 YCL061C, YMR048W Table 13 Annotations of cellular component ontology for biclusters identified by the proposed algorithm...”
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SSO2299 Cobalamin biosynthesis precorrin-3 methylase, putative (cbiF) from Sulfolobus solfataricus P2
35% identity, 49% coverage
TTC0308 No description from Thermus thermophilus HB27
43% identity, 51% coverage
1v9aB / Q5SKH6 Crystal structure of uroporphyrin-iii c-methyl transferase from thermus thermophilus complexed with s-adenyl homocysteine (see paper)
42% identity, 51% coverage
- Ligand: s-adenosyl-l-homocysteine (1v9aB)
Entcl_1738 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from [Enterobacter] lignolyticus SCF1
40% identity, 44% coverage
- Multi-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium
Orellana, PloS one 2017 - “...using cobalamine (Vitamine B-12) as a cofactor. Several genes involved in the synthesis of cobalamine (Entcl_1738, Entcl_1762, Entcl_1763, Entcl_1764, Entcl_1765, Entcl_1766, Entcl_1767, Entcl_1768, and Entcl_1771) were found to be up-regulated in cultures were lignin was added. Furthermore, both laccases are predicted to be extracellular and periplasmic,...”
DSY2227 putative precorrin-2 oxidase from Desulfitobacterium hafniense Y51
35% identity, 44% coverage
WP_012020918 uroporphyrinogen-III C-methyltransferase from Metallosphaera prunae
35% identity, 50% coverage
Q8Y7S4 CbiF protein from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
LM6179_1504 cobalt-precorrin-4 methyltransferase from Listeria monocytogenes 6179
31% identity, 49% coverage
SCO3317 uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase from Streptomyces coelicolor A3(2)
32% identity, 43% coverage
- Comparative analysis of non-coding RNAs in the antibiotic-producing Streptomyces bacteria
Moody, BMC genomics 2013 - “...gene sco2364 sav5807 sven2178 Conserved hypothetical protein sco2685 sav5363 sven2472 Putative ATP-binding protein sco3318 - sco3317 sav4741 - sav4740 sven3179 - sven3180 Putative porphobilinogen deaminase (HemC) / uroporphyrinogen-III synthase (HemD) sco3408 sav4662 sven3260 D-Ala-D-Ala carboxypeptidase sco3671 sav4484 sven3433 DnaK - heat shock protein sco4137 sav4077 sven3895...”
GSU3282 siroheme synthase, N-terminal domain protein from Geobacter sulfurreducens PCA
36% identity, 44% coverage
SHALO_1524, SJPD1_1537, SMN_1546, SMUL_1560 precorrin-4 C(11)-methyltransferase from Sulfurospirillum halorespirans DSM 13726
29% identity, 52% coverage
- Tetrachloroethene respiration in Sulfurospirillum species is regulated by a two-component system as unraveled by comparative genomics, transcriptomics, and regulator binding studies
Esken, MicrobiologyOpen 2020 - “...SHALO_1522 SMUL_1558 SMN_1544 SJPD1_1534 cobaltprecorrin6y C15methyltransferase [decarboxylating] CbiT SHALO_1523 SMUL_1559 SMN_1545 SJPD1_1535 cobaltprecorrin2 C20methyltransferase CbiL SHALO_1524 SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase...”
- “...SMN_1545 SJPD1_1535 cobaltprecorrin2 C20methyltransferase CbiL SHALO_1524 SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551...”
- “...SMN_1544 SJPD1_1534 cobaltprecorrin6y C15methyltransferase [decarboxylating] CbiT SHALO_1523 SMUL_1559 SMN_1545 SJPD1_1535 cobaltprecorrin2 C20methyltransferase CbiL SHALO_1524 SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564...”
- “...SMUL_1558 SMN_1544 SJPD1_1534 cobaltprecorrin6y C15methyltransferase [decarboxylating] CbiT SHALO_1523 SMUL_1559 SMN_1545 SJPD1_1535 cobaltprecorrin2 C20methyltransferase CbiL SHALO_1524 SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528...”
- “...CbiD 6.293549376 4.05651E85 SMUL_1557 CbiE 5.940440235 2.94744E41 SMUL_1558 CbiT 6.377525645 1.48587E40 SMUL_1559 CbiL 6.826321327 3.45861E65 SMUL_1560 CbiF 7.12898108 2.37995E69 SMUL_1561 CbiG 6.327298396 5.61016E85 SMUL_1562 CbiH 6.783888426 2.94934E59 SMUL_1563 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase 5.675055838 1.15417E79 SMUL_1564 CbiJ 4.857598806 6.31887E71 SMUL_1565 lipid A export ATPbinding/permease protein MsbAlike...”
LB_156 precorrin-4 C(11)-methyltransferase from Leptospira interrogans serovar Lai str. 56601
28% identity, 51% coverage
HVO_B0060 cobalamin biosynthesis precorrin-3 methylase from Haloferax volcanii DS2
D4GP62 Cobalt-precorrin-4 C11-methyltransferase from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
33% identity, 49% coverage
SS1G_09177 siroheme synthase from Sclerotinia sclerotiorum 1980 UF-70
31% identity, 50% coverage
- Changes in the Sclerotinia sclerotiorum transcriptome during infection of Brassica napus
Seifbarghi, BMC genomics 2017 - “...- 18.8 - - SS1G_01217 NRPS-like enzyme - - - 5.4 10.5 7.7 3. Other SS1G_09177 siroheme synthase 10.9 8.7 8.3 5.7 - - a Annotation based on the presence of conserved PFAM domains and BLAST reports b Fold change relative to 0 h post inoculation...”
- “...as xenobiotic detoxification [ 81 ]. In the current study, a gene encoding siroheme synthase (SS1G_09177), also known as S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase, was sharply induced during the earliest stages of the infection (1-12 hpi) attesting to the importance of iron sequestration and metabolism. In addition to...”
cbiF / P0A2G9 cobalt precorrin-4 (C11)-methyltransferase (EC 2.1.1.271) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
CBIF_SALTY / P0A2G9 Cobalt-precorrin-4 C(11)-methyltransferase; Cobalt-precorrin-3 methylase; EC 2.1.1.271 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
NP_460974 synthesis of vitamin B12 adenosyl cobalamide precursor from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
33% identity, 50% coverage
CAETHG_1116 precorrin-4 C(11)-methyltransferase from Clostridium autoethanogenum DSM 10061
28% identity, 51% coverage
NGO0806 hypothetical protein from Neisseria gonorrhoeae FA 1090
43% identity, 35% coverage
SCO1855 precorrin-4 C11-methyltransferase from Streptomyces coelicolor A3(2)
34% identity, 49% coverage
- Iron competition triggers antibiotic biosynthesis in Streptomyces coelicolor during coculture with Myxococcus xanthus
Lee, The ISME journal 2020 - “...]. Accordingly, based on the RNA-Seq results, we selected two weak promoters from SCO1797 and SCO1855, which showed constant expression during cell growth (Supplementary Fig. 14a ). The constructed SCO6666 overexpression strain with the SCO1797 promoter actively produced actinorhodin under pure-culture conditions and iron-restricted conditions (Fig....”
- “...were 5.73- and 2.66-fold higher than that in the wild-type and the strain with the SCO1855 promoter, respectively (Supplementary Fig. 14b ). In addition, the complemented strain constructed by introducing SCO6666 overexpression vector into SCO6666 deletion strain showed the same phenotype as the SCO6666 overexpression strain...”
- Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in Streptomyces coelicolor
Xu, Applied and environmental microbiology 2019 - “...sets: seven vitamin B 12 biosynthesis genes, SCO1848 to SCO1850 , SCO1852 , SCO1853 , SCO1855 , and SCO1857 , are ACT upmodulators; six genes involved in branched-chain amino acid metabolism, including three ACT upmodulators, SCO2528 ( leuA ), SCO3345 ( ilvD ), and SCO5512 (...”
TTC0311 No description from Thermus thermophilus HB27
32% identity, 41% coverage
- Effects of Argonaute on Gene Expression in Thermus thermophilus
Swarts, PloS one 2015 - “...(Far) downstream CRISPR 2.7 (reverse orientation) TTC0310 4.2 PAPS reduct ** Phosphoadenosine phosphosulfate reductase No TTC0311 4.3 NAD binding 7, CysG dimeriser, Sirohm synth M ** Uroporphyrin-III C-methyltransferase No TTC0399 4.1 - Hypothetical protein No TTC1169 12.6 DEDD Tnp IS110, Transposase 20, Helix-Hairpin-Helix (HHH) ** Transposase,...”
- “...other in a predicted operon. These and three other genes located on the chromosome (TTC0310, TTC0311 and TTC0399) appear to have no link with CRISPR loci. As we observe only elevated RNA levels under these conditions, it is highly unlikely that Tt Ago interferes with RNA,...”
Caur_0688 siroheme synthase from Chloroflexus aurantiacus J-10-fl
33% identity, 44% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...cbiB (Caur_2573), cbiMNQO (Caur_2574 to Caur_2577), cobU / cobT (Caur_2578), cobN (Caur_2579), cobO (Caur_2580), cysG (Caur_0688) and cbiMNQ (Caur_3680 to Caur_3682) (Genes shared with aerobic and anaerobic pathways shown as aerobic/anaerobic) Heme biosynthesis hemA , hemC , hemD , hemB , hemE , hemL , hemF...”
CAC1380 precorrin-4 methylase cbiF from Clostridium acetobutylicum ATCC 824
26% identity, 50% coverage
PGN_0316 precorrin-4 C11-methyltransferase from Porphyromonas gingivalis ATCC 33277
27% identity, 39% coverage
- Regulon controlled by the GppX hybrid two component system in Porphyromonas gingivalis
Hirano, Molecular oral microbiology 2013 - “...PGN_1053 putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase 0.88 0.04 PGN_0315 precorrin-6x reductase/cobalamin biosynthetic protein CbiD 0.86 0.01 PGN_0316 precorrin-4 C11-methyltransferase 0.86 0.02 PGN_2023 putative phosphoribosylformylglycinamidine cyclo-ligase 0.81 0.03 PGN_1479 hypothetical protein 0.81 0.01 PGN_1749 probable NADPH-quinone reductase 0.78 0.02 PGN_2013 cation efflux system protein 0.77 0.01 PGN_0285 pyridine...”
Entcl_1768 cobalt-precorrin-4 methyltransferase from [Enterobacter] lignolyticus SCF1
32% identity, 50% coverage
- Multi-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium
Orellana, PloS one 2017 - “...Several genes involved in the synthesis of cobalamine (Entcl_1738, Entcl_1762, Entcl_1763, Entcl_1764, Entcl_1765, Entcl_1766, Entcl_1767, Entcl_1768, and Entcl_1771) were found to be up-regulated in cultures were lignin was added. Furthermore, both laccases are predicted to be extracellular and periplasmic, respectively by PSORT [ 53 ] suggesting...”
Achr_f600 precorrin-4 C(11)-methyltransferase from Azotobacter chroococcum NCIMB 8003
32% identity, 50% coverage
TDE0614 precorrin-4 C11-methyltransferase from Treponema denticola ATCC 35405
TDE_0614 precorrin-4 C(11)-methyltransferase from Treponema denticola ATCC 35405
27% identity, 50% coverage
- Transcriptional responses of Treponema denticola to other oral bacterial species
Sarkar, PloS one 2014 - “...the majority of the affected genes were repressed, while induction was only observed for cobM (TDE0614) in response to P. ginigivalis or T. forsythia as well as for TDE1516 (ABC transporter) and TDE2118 (topoisomerase IV) in the presence of P. ginigivalis . Genes repressed in response...”
- “...TDE0308 hypothetical protein 2.56 TDE0567 hypothetical protein 1.75 2.06 TDE0586 membrane protein, putative 1.89 2.09 TDE0614 cobM precorrin-4 C11-methyltransferase 2.91 2.83 TDE0665 pyruvate ferredoxin/flavodoxin oxidoreductase family protein 2.24 2.22 TDE0693 thiD phosphomethylpyrimidine kinase 3.25 TDE0745 grdA glycine reductase complex selenoprotein GrdA 2.37 TDE0753 hypothetical protein 2.03...”
- Characterization of a potential ABC-type bacteriocin exporter protein from Treponema denticola
Tanaka-Kumazawa, BMC oral health 2016 - “...GNAT family 30.0 TDE_0337 glucosamine-6-phosphate deaminase 19.5 TDE_2214 hypothetical protein 17.9 TDE_0561 hypothetical protein 16.0 TDE_0614 precorrin-4 C11-methyltransferase 16.0 TDE_0506 DNA-damage-inducible protein J, putative 15.3 TDE_1848 hypothetical protein 15.2 TDE_0307 hypothetical protein 14.3 TDE_2378 ABC transporter, ATP-binding protein, putative 14.2 TDE_0259 transcriptional regulator, MarR family 12.7...”
BPSL1755 precorrin-4 C11-methyltransferase from Burkholderia pseudomallei K96243
33% identity, 50% coverage
- Microevolution of Burkholderia pseudomallei during an acute infection
Limmathurotsakul, Journal of clinical microbiology 2014 - “...GCACC C35, C43 Intergenic Large 1894305 Deletion of CGACGAC C34 Intergenic Large 2032551 GA C19 BPSL1755 Nonsynonymous substitution, Val132Met Precorrin-4 C11-methyltransferase Porphyrin metabolism Large 2362053 Insertion of ACGGCG C22 Intergenic Large 2485411 GA C39 BPSL2105 Synonymous substitution PspA/IM30 family protein Unknown Large 3572138 CT C19 BPSL3034...”
- Molecular basis of rare aminoglycoside susceptibility and pathogenesis of Burkholderia pseudomallei clinical isolates from Thailand
Trunck, PLoS neglected tropical diseases 2009 - “...factor MbaS defined by mbaF-fmtA-mbaA-mbaI-mbaJ-mbaE - BPSL1781-BPSL1782-BPSL1783-BPSL1784-BPSL1785-BPSL1786-mbaS ; 4 a cluster of 18 genes ( BPSL1755 - BPSL1773 ) encoding a putative aerobic (or late cobalt insertion) vitamin B 12 biosynthetic pathway with an embedded gene ( BPSL1763 ) encoding a putative exported chitinase; 5 arcD...”
- “...Second, immediately adjacent to the malleobactin biosynthetic genes is a cluster of 18 genes ( BPSL1755 - BPSL1773 ) encoding a putative aerobic (or late cobalt insertion) vitamin B 12 biosynthetic pathway [37] . Vitamin B 12 is a known cofactor for numerous enzymes mediating methylation,...”
CBIF_PRIMG / O87696 Cobalt-precorrin-4 C(11)-methyltransferase; Cobalt-precorrin-3 methylase; EC 2.1.1.271 from Priestia megaterium (Bacillus megaterium) (see paper)
O87696 cobalt-precorrin-4 methyltransferase (EC 2.1.1.271) from Priestia megaterium (see 2 papers)
28% identity, 49% coverage
- function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.
catalytic activity: Co-precorrin-4 + S-adenosyl-L-methionine = Co-precorrin-5A + S-adenosyl-L-homocysteine + H(+) (RHEA:26277)
subunit: Homodimer - Bioinformatic Identification of Novel Methyltransferases.
Petrossian, Epigenomics 2009 - “...PSI-BLAST parameter is used with the single sequence of CbiF sequence (UniProtKB: locus CBIF_BACME, accession O87696) against the yeast proteome. Additionally, HHpred is used with the multiple alignment of the corresponding COG group ( COG1798 : Diphthamide biosynthesis methyltransferase DPH5) against the yeast proteome. m The...”
Caur_2566 precorrin-4 C11-methyltransferase from Chloroflexus aurantiacus J-10-fl
32% identity, 46% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...(Caur_2561), cobP / cobU (Caur_2562), cobB/cbiA (Caur_2563), cobA (Caur_2564, Caur_0687), cobJ (Caur_2565), cobM / cbiF (Caur_2566), cobI/cbiL (Caur_2567), cobL / cbiET (Caur_2568), cbiD (Caur_2569), cobH/cbiCH (Caur_2570), cbiK (Caur_2572), cobD / cbiB (Caur_2573), cbiMNQO (Caur_2574 to Caur_2577), cobU / cobT (Caur_2578), cobN (Caur_2579), cobO (Caur_2580), cysG (Caur_0688)...”
1cbfA / O87696 The x-ray structure of a cobalamin biosynthetic enzyme, cobalt precorrin-4 methyltransferase, cbif (see paper)
28% identity, 49% coverage
- Ligand: s-adenosyl-l-homocysteine (1cbfA)
D9QAC4 Precorrin-4 C(11)-methyltransferase from Corynebacterium pseudotuberculosis (strain C231)
29% identity, 50% coverage
PA2948 precorrin-3 methylase from Pseudomonas aeruginosa PAO1
33% identity, 50% coverage
MSMEG_3877 precorrin-4 C11-methyltransferase from Mycobacterium smegmatis str. MC2 155
MSMEG_3877 precorrin-4 C(11)-methyltransferase from Mycolicibacterium smegmatis MC2 155
33% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase A5717_01465 MXEN_00460 cobQ1 Cobyric acid...”
- “...cobL Cobalt-precorrin-6y C5-methyltransferase MAB_2195 95.33 10.65838168 MSMEG_3878 33.24333333 4.282117856 cobM Cobalt-precorrin-4 C11-methyltransferase MAB_2196 190.0066667 34.70278423 MSMEG_3877 40.97333333 4.718308313 cobN Cobalt chelatase MAB_2201 78.53 3.401043957 MSMEG_3864 95.39333333 66.90839808 cobO Cob(I)alamin adenosyltransferase MAB_3156c 94.59 20.00465696 MSMEG_2616 150.34 26.29276136 cobQ1 Cobyric acid synthase MSMEG_2588 69.94666667 36.76729162 cobQ2 Putative amidotransferase...”
BCAL1730 precorrin-4 C11-methyltransferase from Burkholderia cenocepacia J2315
34% identity, 50% coverage
EET03_RS15035 precorrin-4 C(11)-methyltransferase from Mycobacterium innocens
31% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase AWC07_21235 cobQ1 Cobyric acid...”
XA26_35820 precorrin-4 C(11)-methyltransferase from Mycolicibacterium fortuitum
31% identity, 53% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase AWC07_21235...”
PSPPH_2221 precorrin-4 C11-methyltransferase from Pseudomonas syringae pv. phaseolicola 1448A
32% identity, 50% coverage
MMAR_3053 precorrin-4 C11-methyltransferase, CobM from Mycobacterium marinum M
31% identity, 50% coverage
slr0239 precorrin methylase from Synechocystis sp. PCC 6803
42% identity, 29% coverage
PAE3601 uroporphyrin-III C-methyltransferase from Pyrobaculum aerophilum str. IM2
34% identity, 36% coverage
A5717_31670 precorrin-4 C(11)-methyltransferase from Mycolicibacterium porcinum
31% identity, 53% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase A5717_01465 MXEN_00460 cobQ1...”
- “...cbiC Precorrin-8X methylmutase A5717_31640 cbiD cbiE Precorrin-6Y C(5,15)-methyltransferase CDN37_RS01900 A5717_31675 MXEN_01352 AWC27_RS14545 cbiF Precorrin-4 C(11)-methyltransferase A5717_31670 MXEN_01347 cbiG cbiH ATP-binding protein A5717_31645 cbiJ Cobalt-precorrin-6A reductase A5717_31665 MXEN_01342 cbiK cbiL ATP-binding protein A5717_31645 cbiP cbiT Precorrin-6Y-methylase A5717_31675 MXEN_01352 cbiX Sirohydrochlorin ferrochelatase - A5717_10190 SAMEA4434518_00227 MXEN_11286 Urpoporfirynogen III...”
PP_3410 precorrin-4 C11-methyltransferase from Pseudomonas putida KT2440
33% identity, 50% coverage
MPHLCCUG_RS13225 precorrin-4 C(11)-methyltransferase from Mycolicibacterium phlei
32% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Cobalt-precorrin-6reductase A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase A5717_01465 MXEN_00460...”
AWC07_17800 precorrin-4 C(11)-methyltransferase from Mycobacterium gastri
31% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase AWC07_21235 cobQ1...”
- “...cbiB cbiC Precorrin-8X methylmutase AWC07_17765 cbiD cbiE Precorrin-6Y C(5,15)-methyltransferase MCNS_30630 AWC07_17805 H0P51_RS16435 cbiF Precorrin-4 C(11)-methyltransferase AWC07_17800 cbiG - cbiH ATP-binding protein AWC07_17770 cbiJ Cobalt-precorrin-6A reductase AWC07_17795 cbiK cbiL ATP-binding protein AWC07_17770 cbiP cbiT Precorrin-6Y-methylase AWC07_17805 cbiX Sirohydrochlorin ferrochelatase Rv0259c A3N95_07805 AWC07_14695 Urpoporfirynogen III pathway cysG Multifunctional...”
MXEN_01347 precorrin-4 C(11)-methyltransferase from Mycobacterium xenopi RIVM700367
31% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase A5717_01465 MXEN_00460 cobQ1 Cobyric...”
- “...Precorrin-8X methylmutase A5717_31640 cbiD cbiE Precorrin-6Y C(5,15)-methyltransferase CDN37_RS01900 A5717_31675 MXEN_01352 AWC27_RS14545 cbiF Precorrin-4 C(11)-methyltransferase A5717_31670 MXEN_01347 cbiG cbiH ATP-binding protein A5717_31645 cbiJ Cobalt-precorrin-6A reductase A5717_31665 MXEN_01342 cbiK cbiL ATP-binding protein A5717_31645 cbiP cbiT Precorrin-6Y-methylase A5717_31675 MXEN_01352 cbiX Sirohydrochlorin ferrochelatase - A5717_10190 SAMEA4434518_00227 MXEN_11286 Urpoporfirynogen III pathway...”
cg0510 uroporphyrinogen III synthase/methyltransferase from Corynebacterium glutamicum ATCC 13032
29% identity, 41% coverage
SHALO_1532, SJPD1_1544, SMN_1553, SMUL_1568 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Sulfurospirillum halorespirans DSM 13726
29% identity, 32% coverage
- Tetrachloroethene respiration in Sulfurospirillum species is regulated by a two-component system as unraveled by comparative genomics, transcriptomics, and regulator binding studies
Esken, MicrobiologyOpen 2020 - “...SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic acid A,Cdiamide synthase CbiA SHALO_1531 SMUL_1567 SMN_3265 SJPD1_1543 cysteinerich CWC protein SHALO_1532 SMUL_1568 SMN_1553 SJPD1_1544 Precorrin2 dehydrogenase (NADdependent) SHALO_1533 SJPD1_1545 TetRlike transcriptional regulator SMUL_1569 SMN_1554 TetRlike transcriptional regulator, disrupted by transposase SMUL_1570 SMN_1555 ISPsy4, transposition helper protein SMUL_1571 SMN_1556 integrase catalytic region...”
- “...Sh_gDNA 23.83 1.627 Neg Ctrl: SHALO_1504 > SHALO_1505 T774/T775 H 2 O N/A N/A Sample: SHALO_1532 > SHALO_1533 T776/T777 Sh_cDNA 22.48 0.657 RT Ctrl: SHALO_1532 > SHALO_1533 T776/T777 Sh_cDNA N/A N/A Pos Ctrl: SHALO_1532 > SHALO_1533 T776/T777 Sh_gDNA 16.65 0.248 Neg Ctrl: SHALO_1532 > SHALO_1533 T776/T777...”
- “...cobyrinic acid A,Cdiamide synthase CbiA SHALO_1531 SMUL_1567 SMN_3265 SJPD1_1543 cysteinerich CWC protein SHALO_1532 SMUL_1568 SMN_1553 SJPD1_1544 Precorrin2 dehydrogenase (NADdependent) SHALO_1533 SJPD1_1545 TetRlike transcriptional regulator SMUL_1569 SMN_1554 TetRlike transcriptional regulator, disrupted by transposase SMUL_1570 SMN_1555 ISPsy4, transposition helper protein SMUL_1571 SMN_1556 integrase catalytic region SMUL_1572 SMN_1557 TetRlike...”
- “...SJPD1_1542 cobyrinic acid A,Cdiamide synthase CbiA SHALO_1531 SMUL_1567 SMN_3265 SJPD1_1543 cysteinerich CWC protein SHALO_1532 SMUL_1568 SMN_1553 SJPD1_1544 Precorrin2 dehydrogenase (NADdependent) SHALO_1533 SJPD1_1545 TetRlike transcriptional regulator SMUL_1569 SMN_1554 TetRlike transcriptional regulator, disrupted by transposase SMUL_1570 SMN_1555 ISPsy4, transposition helper protein SMUL_1571 SMN_1556 integrase catalytic region SMUL_1572 SMN_1557...”
- “...CCGTTAGAACAATATTTTTTTCTG SMUL_1543 cbiB T739 GCAAAAAAAGCTATTAATGCGG SMUL_1543 cbiB T774 AATATTGCAAAAACATTAGAAGAG SMUL_1540 T775 ATTAAAAATTTTCTTCGATTAAAATTC SMUL_1541 T776 TTAATGTATTTGTAAAACAGCAC SMUL_1568 sirC T777 TTCCCAAAAAAGTTCCATTGC SMUL_1569 T778 AACCATACTAGCACTGCTC SMUL_1572 T779 GGTGAGCCGGATACTCC SMUL_1573 T886 TATGCAGAAGATGATGCAGG SMUL_1539 pceP T887 GTAGCCATGACAATCGGG SMUL_1539 pceP T888 TATATGTTGAAAAATCCTAATAGAG SMUL_1539 pceP T889 TGAAGAACCAGCTTGTATCC SMUL_1539 pceP John Wiley & Sons,...”
- “...SMN_1552 SJPD1_1542 cobyrinic acid A,Cdiamide synthase CbiA SHALO_1531 SMUL_1567 SMN_3265 SJPD1_1543 cysteinerich CWC protein SHALO_1532 SMUL_1568 SMN_1553 SJPD1_1544 Precorrin2 dehydrogenase (NADdependent) SHALO_1533 SJPD1_1545 TetRlike transcriptional regulator SMUL_1569 SMN_1554 TetRlike transcriptional regulator, disrupted by transposase SMUL_1570 SMN_1555 ISPsy4, transposition helper protein SMUL_1571 SMN_1556 integrase catalytic region SMUL_1572...”
An07g02200 uncharacterized protein from Aspergillus niger
26% identity, 45% coverage
C629_RS02545 uroporphyrinogen-III synthase from Corynebacterium glutamicum SCgG2
28% identity, 41% coverage
- Modular control of multiple pathways of Corynebacterium glutamicum for 5-aminolevulinic acid production
Ge, AMB Express 2021 - “...regulon C629_RS02555 hemB Porphobilinogen synthase 0.14 1.494 1.364 C629_RS02485 hemC Hydroxymethylbilane synthase 1.50 1.54 0.09 C629_RS02545 hemD Bifunctional uroporphyrinogen-III C-methyltransferase/uroporphyrinogen-III synthase 1.66 1.36 +0.24 C629_RS02575 hemE Uroporphyrinogen decarboxylase 0.54 0.69 0.20 C629_RS11225 hemN Coproporphyrinogen III oxidase +0.99 +0.31 0.73 C629_RS02580 hemY Protoporphyrinogen oxidase 1.16 1.27 0.16...”
Glov_3650 precorrin-4 C11-methyltransferase from Geobacter lovleyi SZ
32% identity, 50% coverage
Rv2071c Probable precorrin-4 C11-methyltransferase CobM from Mycobacterium tuberculosis H37Rv
31% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Cobalt-precorrin-6reductase Rv2070c MAB_2197 MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide...”
- “...419.98 124.7825583 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c 51.73666667 2.388616522 56.36333333 44.1996458 219.44 310.3350241 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c 32.80333333 1.224100577 63.16666667 39.84209192 0 0 cobN Cobalt chelatase Rv2062c 101.4866667 3.59177146 46.24666667 13.10098554 167.3266667 118.4526528 cobO Cob(I)alamin adenosyltransferase Rv2849c 77.32666667 3.099519676 74.58333333 10.26311302 507.9333333 458.6724143 cobQ1 Cobyric acid synthase...”
- Implementation of homology based and non-homology based computational methods for the identification and annotation of orphan enzymes: using Mycobacterium tuberculosis H37Rv as a case study
Sinha, BMC bioinformatics 2020 - “...and chlorophyll metabolism) 1.3.1.54 (Precorrin-6A reductase) Rv2070c 2.5.1.6 (Methionine adenosyltransferase) Rv1392 2.1.1.133 (Precorrin-4 C (11)-methyltransferase) Rv2071c 2.3.2.10 (UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase/ Peptidoglycan biosynthesis) 6.1.1.14 (GlycinetRNA ligase) Rv0041 6.1.1.7 AlaninetRNA ligase Rv2555c 6.3.2.10 (MurF synthetase) Rv2157c 6.3.1.2 (Glutamine synthetase) Rv2222c 1.14.13.83 (Precorrin-3B synthase/porphyrin and chlorophyll metabolism) 2.1.1.131 (Precorrin-3B C(17)-methyltransferase)...”
- Purifying Selective Pressure Suggests the Functionality of a Vitamin B12 Biosynthesis Pathway in a Global Population of Mycobacterium tuberculosis
Minias, Genome biology and evolution 2018 - “...cobC Rv2231c 1,095 3.56 0.63 3 3 cobQ1 Rv0255c 1,485 3.43 0.62 4 4 cobM Rv2071c 756 3.44 0.61 7 7 Rv2067c 1,224 3.02 0.61 7 6 cobG Rv2064 1,092 2.93 0.54 4 3 cbiX Rv0259c 744 4.03 0.49 2 2 mutB Rv1493 2,253 2.57 0.47...”
- Comparative Genomic Analysis of Two Clonally Related Multidrug Resistant Mycobacterium tuberculosis by Single Molecule Real Time Sequencing
Leung, Frontiers in cellular and infection microbiology 2017 - “...mutation at rv3303c ( lpdA) V44I and a 6-bp inframe deletion at codon 6768 in rv2071c ( cobM) in the post-treatment strain. Multiple sequence alignment showed that these mutations were occurring at highly conserved regions among pathogenic mycobacteria. Using structural-based and sequence-based algorithms, we further predicted...”
- “...novel gene targets with differential mutations. Gene Orientation Oligonucleotide sequence rv0888 Forward 5-ATGACTTGCTCAATGCCCTG-3 Reverse 5-GGTCACGATGCCATGCTG-3 rv2071c Forward 5-GACGGTCTATTTCATCGGAGC-3 Reverse 5-GGCGGTATGGGTGTGGAC-3 rv2921c Forward 5-GACGAGGTGTTGCTGGTGC-3 Reverse 5-GGGTCGCCACCATCAGAC-3 rv3303c Forward 5-CTAGGTTATGGGCTGTGGTGAC-3 Reverse 5-CTCATGCTCACTGGTGGAG-3 Alignment analysis Amino acid sequences of target gene candidates in MTB H37Rv were identified in KEGG...”
- iniBAC induction Is Vitamin B12- and MutAB-dependent in Mycobacterium marinum
Boot, The Journal of biological chemistry 2016 - “...Rv2848 Rv1492 Rv1493 Rv1496 Rv2062c Rv2065 Rv2066 Rv2070c Rv2071c Rv2072c Rv0254c Rv2207 Rv2231c Rv2236c Rv1013 none Rv0819 3153 684 5616 1377 1878 2262 1011...”
- High Persister Mutants in Mycobacterium tuberculosis
Torrey, PloS one 2016 - “...Rv1387 PPE family protein 1563341 C T P525S Rv1395 transcriptional regulator 1571669 C T P208L Rv2071c precorrin-4 c11-methyltransferase cobM 2328463 C T A172V Rv2249c glycerol-3-phosphate dehydrogenase glpD1 2523381 C T R471W Rv2423 hypothetical protein 2719708 G A V38M KL2802 Rv0351 chaperone grpE 422212 G A M168I...”
- Differential gene expression between Mycobacterium bovis and Mycobacterium tuberculosis
Rehren, Tuberculosis (Edinburgh, Scotland) 2007 - “...Rv1977 6.9 0.0452 7 Conserved hypothetical protein Rv2071c cobM 3.7 0.00765 Precorrin-4 c11-methyltransferase Rv2073c 4.8 0.032 9 Probable shortchain...”
MSMEG_0954 uroporphyrinogen-III synthase from Mycolicibacterium smegmatis MC2 155
27% identity, 42% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...CDN37_RS17875 MPHLCCUG_RS22010 A5717_22195 SAMEA4434518_00497 MXEN_04668 AWC27_RS07580 MSMEG_0953 hemD Uroporphyrinogen III methyltransferase/synthase - A5717_10195 SAMEA4434518_00498 MXEN_04663 MSMEG_0954 hemL Glutamate-1-semialdehyde 2,1-aminomutase MKAN_RS17800 CDN37_RS18005 MPHLCCUG_RS21920 A5717_22280 SAMEA4434518_00514 MXEN_04593 AWC27_RS07655 MSMEG_0969 hemY ChlI component of cobalt chelatase SAMEA4434518_01694 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase CDN37_RS02225 MPHLCCUG_RS15920 A5717_31970 SAMEA4434518_01721 MXEN_01507...”
- “...201.9733333 27.76189895 hemC Porphobilinogen deaminase MAB_3992c 638.4233333 82.95156076 MSMEG_0953 374.95 313.6007022 hemD Uroporphyrinogen III methyltransferase/synthase MSMEG_0954 317.5766667 44.41745753 hemL Glutamate-1-semialdehyde 2,1-aminomutase MSMEG_0969 110.7066667 25.2243619 hemY ChlI component of cobalt chelatase MAB_2985c 196.3366667 1.652099674 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase MAB_2129 605.9966667 39.45958101 MSMEG_0093 94.66 39.15167557...”
MSMEG_0954 uroporphyrinogen-III synthase from Mycobacterium smegmatis str. MC2 155
27% identity, 44% coverage
- c-di-AMP Accumulation Regulates Growth, Metabolism, and Immunogenicity of Mycobacterium smegmatis
Ning, Frontiers in microbiology 2022 - “...smegmatis significantly upregulated the expression of porphyrin-metabolizing enzymes in 3-day culture, including MSMEG_2618 (uroporphyrin-III C-methyltransferase), MSMEG_0954 (uroporphyrinogen-III synthase), and MSMEG_2780 (uroporphyrinogen decarboxylase) ( Figure 6C ), and downregulated the expression of MSMEG_4525 (putative oxygen-independent coproporphyrinogen III oxidase) ( Figure 6C ), which may result in more...”
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...CDN37_RS17875 MPHLCCUG_RS22010 A5717_22195 SAMEA4434518_00497 MXEN_04668 AWC27_RS07580 MSMEG_0953 hemD Uroporphyrinogen III methyltransferase/synthase - A5717_10195 SAMEA4434518_00498 MXEN_04663 MSMEG_0954 hemL Glutamate-1-semialdehyde 2,1-aminomutase MKAN_RS17800 CDN37_RS18005 MPHLCCUG_RS21920 A5717_22280 SAMEA4434518_00514 MXEN_04593 AWC27_RS07655 MSMEG_0969 hemY ChlI component of cobalt chelatase SAMEA4434518_01694 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase CDN37_RS02225 MPHLCCUG_RS15920 A5717_31970 SAMEA4434518_01721 MXEN_01507...”
- “...201.9733333 27.76189895 hemC Porphobilinogen deaminase MAB_3992c 638.4233333 82.95156076 MSMEG_0953 374.95 313.6007022 hemD Uroporphyrinogen III methyltransferase/synthase MSMEG_0954 317.5766667 44.41745753 hemL Glutamate-1-semialdehyde 2,1-aminomutase MSMEG_0969 110.7066667 25.2243619 hemY ChlI component of cobalt chelatase MAB_2985c 196.3366667 1.652099674 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase MAB_2129 605.9966667 39.45958101 MSMEG_0093 94.66 39.15167557...”
- The NnaR orphan response regulator is essential for the utilization of nitrate and nitrite as sole nitrogen sources in mycobacteria
Antczak, Scientific reports 2018 - “...NnaR does not possess HemD activity 14 . M . smegmatis possesses another HemD paralog, MSMEG_0954, which is likely the actual uroporphyrinogen-III synthase. Interestingly, NnaR is required to support the expression of the bacterial truncated haemoglobin, trHbN ( msmeg_5765 ), which is one of the highest-affinity...”
BC1428 Ferrochelatase from Bacillus cereus ATCC 14579
31% identity, 39% coverage
Newbould305_0486 NAD(P)-binding protein from Staphylococcus aureus subsp. aureus str. Newbould 305
30% identity, 35% coverage
- Recombination-mediated remodelling of host-pathogen interactions during Staphylococcus aureus niche adaptation
Spoor, Microbial genomics 2015 - “...protein Newbould305_0563 Rhodanese domain sulfur transferase Newbould305_0534 Acetyltransferase Newbould305_0564 Pyrrolidone carboxylate peptidase Newbould305_0641 Amidohydrolase cysG Newbould305_0486 Precorrin-2 dehydrogenase Newbould305_0793 Hexitol dehydrogenase Newbould305_0736 RND transporter Newbould305_0757 NADH-dependent dehydrogenase Newbould305_0815 Ribose transporter RbsU Newbould305_0748 -Glutamyltransferase Newbould305_0719 4-Phosphopantetheinyl transferase * Locus tags according to annotations in strain MW2 (GenBank...”
MKAN_RS01815 precorrin-4 C(11)-methyltransferase from Mycobacterium kansasii ATCC 12478
30% identity, 53% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MSMEG_3873 cobK Cobalt-precorrin-6reductase A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase...”
CDN37_RS01895 precorrin-4 C(11)-methyltransferase from Mycobacterium persicum
31% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...cobK Cobalt-precorrin-6reductase A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864 cobO Cob(I)alamin adenosyltransferase MKAN_RS23620 CDN37_RS23990 MPHLCCUG_RS15725 A5717_05675 MXEN_03569 AWC27_RS04130 MSMEG_2616 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase A5717_01465...”
MCNS_30620 precorrin-4 C(11)-methyltransferase from Mycobacterium conspicuum
30% identity, 50% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MAB_2197 MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase...”
AFUA_7G05680 siroheme synthase Met8, putative from Aspergillus fumigatus Af293
28% identity, 43% coverage
XAC2157 uroporphyrin-III C-methyltransferase from Xanthomonas axonopodis pv. citri str. 306
44% identity, 21% coverage
- The sulfur/sulfonates transport systems in Xanthomonas citri pv. citri
Pereira, BMC genomics 2015 - “...S. typhimurium. (A) Positioning of the cysG genes in the X. citri genome. Xac2159 and Xac2157 genes, encoding respectively, an uroporphyrin-III C-methyl transferase and a syroheme synthase, are closely located in opposite directions. The third gene, Xac3341, encoding another syroheme synthase is located in the same...”
Mchl_5685 precorrin-4 C11-methyltransferase from Methylobacterium chloromethanicum CM4
34% identity, 51% coverage
DSY4068 cobalt-precorrin-4 C(11)-methyltransferase from Desulfitobacterium hafniense Y51
28% identity, 55% coverage
MAB_3991c Possible Uroporphyrin-III C-methyltransferase from Mycobacterium abscessus ATCC 19977
29% identity, 44% coverage
Clocel_2227 precorrin-4 C(11)-methyltransferase from Clostridium cellulovorans 743B
26% identity, 49% coverage
cobM / P21922 precorrin-4 (C11)-methyltransferase (EC 2.1.1.133) from Sinorhizobium sp. (see paper)
P21922 precorrin-4 C11-methyltransferase (EC 2.1.1.133) from Pseudomonas denitrificans (nom. rej.) (see 2 papers)
29% identity, 51% coverage
MAB_2196 precorrin-4 C(11)-methyltransferase from Mycobacteroides abscessus ATCC 19977
29% identity, 53% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Rv2070c MAB_2197 MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate...”
- “...MSMEG_3875 40.99 6.827935266 cobL Cobalt-precorrin-6y C5-methyltransferase MAB_2195 95.33 10.65838168 MSMEG_3878 33.24333333 4.282117856 cobM Cobalt-precorrin-4 C11-methyltransferase MAB_2196 190.0066667 34.70278423 MSMEG_3877 40.97333333 4.718308313 cobN Cobalt chelatase MAB_2201 78.53 3.401043957 MSMEG_3864 95.39333333 66.90839808 cobO Cob(I)alamin adenosyltransferase MAB_3156c 94.59 20.00465696 MSMEG_2616 150.34 26.29276136 cobQ1 Cobyric acid synthase MSMEG_2588 69.94666667 36.76729162...”
MAB_2196 Probable precorrin-4 C11-methyltransferase CobM from Mycobacterium abscessus ATCC 19977
29% identity, 53% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...Rv2070c MAB_2197 MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate...”
- “...MSMEG_3875 40.99 6.827935266 cobL Cobalt-precorrin-6y C5-methyltransferase MAB_2195 95.33 10.65838168 MSMEG_3878 33.24333333 4.282117856 cobM Cobalt-precorrin-4 C11-methyltransferase MAB_2196 190.0066667 34.70278423 MSMEG_3877 40.97333333 4.718308313 cobN Cobalt chelatase MAB_2201 78.53 3.401043957 MSMEG_3864 95.39333333 66.90839808 cobO Cob(I)alamin adenosyltransferase MAB_3156c 94.59 20.00465696 MSMEG_2616 150.34 26.29276136 cobQ1 Cobyric acid synthase MSMEG_2588 69.94666667 36.76729162...”
PC2DH_METBF / Q46CH4 Precorrin-2 dehydrogenase; Mba_PC2_DH; EC 1.3.1.76 from Methanosarcina barkeri (strain Fusaro / DSM 804) (see paper)
28% identity, 39% coverage
- function: Involved in the archaeal biosynthesis of heme. Catalyzes the oxiation of precorrin-2 into sirohydroclorin.
catalytic activity: precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+) (RHEA:15613)
subunit: Homodimer - Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea.
Pfeiffer, Genes 2021 - “...- function unassigned; no GSP; distantly related to paralogs 3a HVO_2312 sirC yes/no 31% Mbar_A1461 Q46CH4 [ 166 ] 21197080 precorrin-2 DH; no analysis for Fe-chelatase 3a HVO_2312 (cont.) yes/no 29% STM3477 P25924 [ 167 ] [ 168 ] 14595395 32054833 matches to the N-term domain...”
MET8_SCHPO / O14172 Siroheme biosynthesis protein met8; EC 1.3.1.76; EC 4.99.1.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
SPAC4D7.06c siroheme synthase (predicted) from Schizosaccharomyces pombe
29% identity, 36% coverage
H0P51_RS16430 precorrin-4 C(11)-methyltransferase from Mycobacterium vicinigordonae
28% identity, 49% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201 A3N95_10105 MCNS_30500 XA26_35720 AWC07_17750 H0P51_RS16365 EET03_RS14960 cobO Cob(I)alamin adenosyltransferase Rv2849c MAB_3156c MCNS_15890 XA26_25260 AWC07_16315 H0P51_RS10510 EET03_RS08685 cobP Adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase AWC07_21235 cobQ1 Cobyric...”
SHALO_1526, SJPD1_1539, SMN_1548, SMUL_1562 precorrin-3B C(17)-methyltransferase from Sulfurospirillum diekertiae
26% identity, 50% coverage
- Tetrachloroethene respiration in Sulfurospirillum species is regulated by a two-component system as unraveled by comparative genomics, transcriptomics, and regulator binding studies
Esken, MicrobiologyOpen 2020 - “...SHALO_1524 SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease...”
- “...SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike SHALO_1530 SMUL_1566 SMN_1552 SJPD1_1542 cobyrinic acid A,Cdiamide synthase...”
- “...SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein MsbAlike...”
- “...SMUL_1560 SMN_1546 SJPD1_1536 cobaltprecorrin4 C11methyltransferase CbiF SHALO_1525 SMUL_1561 SMN_1547 SJPD1_1537 cobalamin biosynthesis protein CbiG SHALO_1526 SMUL_1562 SMN_1548 SJPD1_1538 cobaltprecorrin3b C17methyltransferase CbiH SHALO_1527 SMUL_1563 SMN_1549 SJPD1_1539 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase SHALO_1528 SMUL_1564 SMN_1550 SJPD1_1540 cobaltprecorrin6x reductase CbiJ SHALO_1529 SMUL_1565 SMN_1551 SJPD1_1541 lipid A export ATPbinding/permease protein...”
- “...CbiT 6.377525645 1.48587E40 SMUL_1559 CbiL 6.826321327 3.45861E65 SMUL_1560 CbiF 7.12898108 2.37995E69 SMUL_1561 CbiG 6.327298396 5.61016E85 SMUL_1562 CbiH 6.783888426 2.94934E59 SMUL_1563 uroporphyrinogenIII methyltransferase / UroporphyrinogenIII synthase 5.675055838 1.15417E79 SMUL_1564 CbiJ 4.857598806 6.31887E71 SMUL_1565 lipid A export ATPbinding/permease protein MsbAlike 3.373444956 2.04003E15 SMUL_1566 CbiA 4.123923514 6.67467E31 SMUL_1567 cysteinerich...”
BTB_RS10805 precorrin-2 dehydrogenase from Bacillus thuringiensis Bt407
29% identity, 33% coverage
SIRC_BACSU / O34813 Precorrin-2 dehydrogenase; EC 1.3.1.76 from Bacillus subtilis (strain 168) (see 2 papers)
32% identity, 28% coverage
- function: Catalyzes the dehydrogenation of precorrin-2 to form sirohydrochlorin which is used as a precursor in both siroheme biosynthesis and in the anaerobic branch of adenosylcobalamin biosynthesis.
catalytic activity: precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+) (RHEA:15613)
disruption phenotype: Cells lacking this gene are unable to grow on minimal medium with nitrate as nitrogen source. Growth is restored only after addition of both cysteine and ammonia to the medium.
MXEN_04663 uroporphyrinogen-III synthase from Mycobacterium xenopi RIVM700367
28% identity, 44% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MKAN_RS17645 CDN37_RS17875 MPHLCCUG_RS22010 A5717_22195 SAMEA4434518_00497 MXEN_04668 AWC27_RS07580 MSMEG_0953 hemD Uroporphyrinogen III methyltransferase/synthase - A5717_10195 SAMEA4434518_00498 MXEN_04663 MSMEG_0954 hemL Glutamate-1-semialdehyde 2,1-aminomutase MKAN_RS17800 CDN37_RS18005 MPHLCCUG_RS21920 A5717_22280 SAMEA4434518_00514 MXEN_04593 AWC27_RS07655 MSMEG_0969 hemY ChlI component of cobalt chelatase SAMEA4434518_01694 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase CDN37_RS02225 MPHLCCUG_RS15920 A5717_31970 SAMEA4434518_01721...”
EQ812_02400 NAD(P)-binding protein from Staphylococcus lugdunensis
27% identity, 35% coverage
BC2132 Ferrochelatase from Bacillus cereus ATCC 14579
29% identity, 33% coverage
Rv0511 PROBABLE UROPORPHYRIN-III C-METHYLTRANSFERASE HEMD (UROPORPHYRINOGEN III METHYLASE) (UROGEN III METHYLASE) (SUMT) (UROGEN III METHYLASE) (UROM) from Mycobacterium tuberculosis H37Rv
28% identity, 43% coverage
- Acquiring of photosensitivity by Mycobacterium tuberculosis in vitro and inside infected macrophages is associated with accumulation of endogenous Zn-porphyrins
Shleeva, Scientific reports 2024 - “...of porphyrins for actively growing cells: uroporphyrinogen-III synthase ( Rv0260c ) and uroporphyrin-III C-methyltransferase ( Rv0511 ) (Fig. S8 )SAM-dependent, membrane-bound methyltransferase methylating uroporphyrin III to form sirohydrochlorin 31 . The effect of ALA supplementation was also analyzed in relation to the gene expression level of...”
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...EET03_RS02885 hemC Porphobilinogen deaminase Rv0510 MAB_3992c MCNS_03970 XA26_52020 AWC07_11795 H0P51_RS04260 EET03_RS02875 hemD Uroporphyrinogen III methyltransferase/synthase Rv0511 MCNS_03980 AWC07_14690 hemL Glutamate-1-semialdehyde 2,1-aminomutase Rv0524 MCNS_04110 XA26_51830 AWC07_11675 H0P51_RS04340 EET03_RS03025 hemY ChlI component of cobalt chelatase Rv2850c MAB_2985c MCNS_17540 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase Rv2124c MAB_2129 MCNS_30990...”
- “...114.4782576 hemC Porphobilinogen deaminase Rv0510 627.0233333 64.43651415 435.5433333 115.7868324 161.2566667 114.1554689 hemD Uroporphyrinogen III methyltransferase/synthase Rv0511 640.4733333 56.28158037 342.2533333 64.04123949 138.85 9.488702054 hemL Glutamate-1-semialdehyde 2,1-aminomutase Rv0524 437.8066667 25.11909809 114.9666667 72.38097832 391.8 135.6539762 hemY ChlI component of cobalt chelatase Rv2850c 135.66 6.78823001 38.01666667 3.454488224 81.53333333 115.3055458 Vitamin...”
- Implementation of homology based and non-homology based computational methods for the identification and annotation of orphan enzymes: using Mycobacterium tuberculosis H37Rv as a case study
Sinha, BMC bioinformatics 2020 - “...reactions in a pathway are unique). The hole filling protocol ranked the proteins Rv2064 and RV0511 as the top-ranked candidates for the enzymes 1.14.13.83 and 2.1.1.152, respectively. A more detailed analysis of these proteins tells us that both are unreviewed entries in UniProt with only electronic...”
- “...(PF03460: NIR_SIR_ferr) providing additional evidence for the functionality for the candidate protein Rv2064. Similarly, for Rv0511 which is ranked 1 for the function, EC 2.1.1.152, Precorrin-6A synthase, the presence of the same Pfam domain TP_methylase (PF00590) supports the prediction using our method. These annotations, if experimentally...”
- Purifying Selective Pressure Suggests the Functionality of a Vitamin B12 Biosynthesis Pathway in a Global Population of Mycobacterium tuberculosis
Minias, Genome biology and evolution 2018 - “...cobL Rv2072c 1,173 4.18 0.87 5 5 metH Rv2124c 3,579 2.93 0.84 14 13 hemD Rv0511 1,698 3.47 0.84 5 4 nrdZ Rv0570 2,054 3.65 0.76 11 10 cobK Rv2070c 735 5.85 0.72 5 3 cobB Rv2848c 1,374 1.97 0.71 7 6 che1 Rv2393 846 3.90...”
- Characterizing the pocketome of Mycobacterium tuberculosis and application in rationalizing polypharmacological target selection
Anand, Scientific reports 2014 - “...are highly treatment-experienced or have HIV-1 strains resistant to multiple protease inhibitors Ritonavir (DB00503;RIT) 0.68 Rv0511; Rv0627; Rv0904c; Rv1092c; Rv1220c; Rv1237; Rv1663; Rv1843c; Rv2026c.; Rv2335; Rv2388c; Rv2678c; Rv2689c; Rv2842c; Rv2935; Rv3303c; Rv3509c; Rv3582c; Rv3825c; Rv3886c; Indicated in combination with other antiretroviral agents for the treatment of...”
- “...Rv3790; Rv3882c; Rv3886c; For treatment of external genital warts (Condyloma acuminatum). Lapatinib (DB01259;FMM) 0.56 Rv0237; Rv0511; Rv0527; Rv0545c; Rv0554; Rv0555; Rv0570; Rv0724; Rv0732; Rv0904c; Rv1127c; Rv1181; Rv1220c; Rv1485; Rv1505c; Rv1530; Rv1650; Rv1663; Rv1843c; Rv1872c; Rv1905c; Rv2165c; Rv2231c; Rv2439c; Rv2524c; Rv2610c; Rv2613c; Rv2623; Rv2678c; Rv2897c; Rv2902c; Rv2941;...”
- A novel nucleoid-associated protein of Mycobacterium tuberculosis is a sequence homolog of GroEL
Basu, Nucleic acids research 2009 - “...S/No. E. coli M. tuberculosis e -value Remarks 1 HNS Rv2477c 7.2 Absent 2 Hfq Rv0511 0.55 Absent 3 StpA Rv3296 1.4 Absent 4 Dps Rv1522c 0.14 Absent 5 HupA Rv2986 (HupB) 8 e Best BLAST hits 6 HupB Rv2986 (HupB) 4 e 11 for all...”
SAOUHSC_02945 siroheme synthase, putative from Staphylococcus aureus subsp. aureus NCTC 8325
SAUSA300_2553 putative siroheme synthase from Staphylococcus aureus subsp. aureus USA300_FPR3757
31% identity, 35% coverage
DVU2748 precorrin-4 C11-methyltransferase from Desulfovibrio vulgaris Hildenborough
32% identity, 40% coverage
SA2412 precorrin-2 dehydrogenase from Staphylococcus aureus subsp. aureus N315
31% identity, 35% coverage
GBAA2142 siroheme synthase domain protein from Bacillus anthracis str. 'Ames Ancestor'
27% identity, 33% coverage
MCNS_03980 uroporphyrinogen-III synthase from Mycobacterium conspicuum
26% identity, 44% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...hemC Porphobilinogen deaminase Rv0510 MAB_3992c MCNS_03970 XA26_52020 AWC07_11795 H0P51_RS04260 EET03_RS02875 hemD Uroporphyrinogen III methyltransferase/synthase Rv0511 MCNS_03980 AWC07_14690 hemL Glutamate-1-semialdehyde 2,1-aminomutase Rv0524 MCNS_04110 XA26_51830 AWC07_11675 H0P51_RS04340 EET03_RS03025 hemY ChlI component of cobalt chelatase Rv2850c MAB_2985c MCNS_17540 Vitamin B12 dependent enzymes metH 5-Methyltetrahydrofolatehomocysteine methyltransferase Rv2124c MAB_2129 MCNS_30990 AWC07_11205...”
3ndcA / O68100 Crystal structure of precorrin-4 c11-methyltransferase from rhodobacter capsulatus
29% identity, 51% coverage
- Ligand: s-adenosyl-l-homocysteine (3ndcA)
Mchl_5690 precorrin-2 C20-methyltransferase from Methylobacterium chloromethanicum CM4
31% identity, 31% coverage
LB157 precorrin methylase from Leptospira interrogans serovar lai str. 56601
LB_157 precorrin-3B C(17)-methyltransferase from Leptospira interrogans serovar Lai str. 56601
29% identity, 47% coverage
HMPREF9321_0616 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Veillonella atypica ACS-049-V-Sch6
27% identity, 43% coverage
sirC / P61818 precorrin-2 dehydrogenase monomer (EC 1.3.1.76) from Priestia megaterium (see 3 papers)
SIRC_PRIMG / P61818 Precorrin-2 dehydrogenase; EC 1.3.1.76 from Priestia megaterium (Bacillus megaterium) (see 2 papers)
P61818 precorrin-2 dehydrogenase (EC 1.3.1.76) from Priestia megaterium (see 2 papers)
28% identity, 31% coverage
- function: Catalyzes the dehydrogenation of precorrin-2 to form sirohydrochlorin which is used as a precursor in both siroheme biosynthesis and in the anaerobic branch of adenosylcobalamin biosynthesis. It is unable to oxidize precorrin-3.
catalytic activity: precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+) (RHEA:15613)
subunit: Homodimer. - Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea.
Pfeiffer, Genes 2021 - “...domain which is bifunctional as precorrin-2 DH and Fe-chelatase 3a HVO_2312 (cont.) yes/no 29% - P61818 [ 163 ] [ 169 ] 12408752 18588505 precorrin-2 DH; devoid of Fe-chelatase activity 3b HVO_B0061 cbiL no 32% STM2024 Q05593 [ 170 ] 1451790 equivalent reaction on cobalt-free substrate...”
lmo1199 similar to precorrin methylase from Listeria monocytogenes EGD-e
33% identity, 33% coverage
- Comparison between Listeria sensu stricto and Listeria sensu lato strains identifies novel determinants involved in infection
Schardt, Scientific reports 2017 - “...validated via qRT-PCR for the selected genes lmo1146, pocR (lmo1150), pduC (lmo1153), lmo1190 and cbiA (lmo1199) in stationary phase (Table 2 ). In exponential phase, there is a discrepancy between qRT-PCR and NGS results due to the higher sensitivity of qRT-PCR and the overall low number...”
- “...Relative transcription in percent of the genes lmo1146, pocR (lmo1150), pduC (lmo1153), lmo1190 and cbiH (lmo1199) during growth in BHI with 10mM 1,2-PD (BHI+1,2-PD), in BHI with 10mM 1,2-PD and 25nM cobalamin (BHI+1,2-PD+B 12 ), or in BHI. Gene Stationary Phase Exponential Phase BHI* BHI+1,2-PD BHI+1,2-PD+B...”
- Genome-wide analyses reveal lineage specific contributions of positive selection and recombination to the evolution of Listeria monocytogenes
Orsi, BMC evolutionary biology 2008 - “..., lmo2348 , lmo2566 , lmo0487 , lmo0878 , lmo1004 , lmo1011 , cbiH ( lmo1199 ). "x" indicate substitutions inferred to have occurred in the respective branches. Red lines represent the probability for each nucleotide to have been imported by means of recombination. Values at...”
LMOf2365_1208 precorrin-3B C17-methyltransferase from Listeria monocytogenes str. 4b F2365
33% identity, 33% coverage
LM6179_1506 precorrin-3B C(17)-methyltransferase from Listeria monocytogenes 6179
33% identity, 33% coverage
DSY4066 cobalt-precorrin-3B C(17)-methyltransferase from Desulfitobacterium hafniense Y51
27% identity, 46% coverage
BLBBGE_281 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Blattabacterium sp. (Blattella germanica) str. Bge
25% identity, 36% coverage
Caur_2565 precorrin-3B C17-methyltransferase from Chloroflexus aurantiacus J-10-fl
38% identity, 17% coverage
- Complete genome sequence of the filamentous anoxygenic phototrophic bacterium Chloroflexus aurantiacus
Tang, BMC genomics 2011 - “...cbiP (Caur_2560), N -transferase (Caur_2561), cobP / cobU (Caur_2562), cobB/cbiA (Caur_2563), cobA (Caur_2564, Caur_0687), cobJ (Caur_2565), cobM / cbiF (Caur_2566), cobI/cbiL (Caur_2567), cobL / cbiET (Caur_2568), cbiD (Caur_2569), cobH/cbiCH (Caur_2570), cbiK (Caur_2572), cobD / cbiB (Caur_2573), cbiMNQO (Caur_2574 to Caur_2577), cobU / cobT (Caur_2578), cobN (Caur_2579),...”
SMc03191 PROBABLE PRECORRIN-2 C20-METHYLTRANSFERASE PROTEIN from Sinorhizobium meliloti 1021
30% identity, 44% coverage
cobI / P21639 CobI (EC 2.1.1.130) from Sinorhizobium sp. (see 2 papers)
30% identity, 31% coverage
HVO_B0058 precorrin-3B C17-methyltransferase from Haloferax volcanii DS2
D4GP60 Cobalt-factor-III C17-methyltransferase from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
33% identity, 28% coverage
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea
Pfeiffer, Genes 2021 - “...Q05590 [ 171 ] [ 172 ] 9331403 16198574 equivalent reaction on cobalt-free substrate 3b HVO_B0058 cbiH1 special 32% - O87689 [ 160 ] 23922391 corresponds to N-term of O87689 which has a C-term extension; more distant to O87689 than CbiH2 3b HVO_B0058 (cont.) no 30%...”
- “...of reaction is indicated (oxidoreductase and ~CH3, indicating a methylation reaction). The question mark after HVO_B0058 indicates that this protein, currently co-attributed to EC 2.1.1.272, is a candidate for the yet-unassigned EC 2.1.1.195 reaction. We note that haloarchaea might use a deviating biosynthesis pathway, e.g., by...”
- Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes
van, Nucleic acids research 2018 - “...140 PrmC D4GW96 0.0021 Class I translation termination factor methyltransferase YES Class I 149 CbiH1 D4GP60 0.0019 Precorrin-3B C17-methyltransferase Not tested Class III 211 CbiF D4GP62 0.0015 Cobalamin biosynthesis precorrin-3 methylase Not tested Class III 240 HVO_1534 D4GYH8 0.0014 Unknown Not tested Class I 260 HVO_1715...”
SSO2306 Cobalamin biosynthesis precorrin-3 methylase, putative (cbiH) from Sulfolobus solfataricus P2
24% identity, 52% coverage
Synpcc7942_1853 precorrin-2 C20-methyltransferase from Synechococcus elongatus PCC 7942
32% identity, 30% coverage
- Blueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942
Delaye, BMC genomics 2011 - “...antiporter subunit C Oxidative phosphorylation Synpcc7942_1851 ferredoxin--nitrite reductase Nitrogen metabolism Synpcc7942_1852 precorrin-8X methylmutase Chlorophyll metabolism Synpcc7942_1853 precorrin-2 C20-methyltransferase Chlorophyll metabolism Synpcc7942_1854 precorrin-3 methyltransferase Chlorophyll metabolism Synpcc7942_1855 cobyrinic acid a,c-diamide synthase Chlorophyll metabolism Synpcc7942_2151 cellulose synthase (UDP-forming) Starch and sucrose metabolism Synpcc7942_2238 glucose transport protein Transport Synpcc7942_2436...”
- “...Maby transferred among Cyanobacteria. Synpcc7942_1852 precorrin-8X methylmutase yes yes 35 -1 Likely transferred among Cyanobacteria. Synpcc7942_1853 cobalt-factor II C20-methyltransferase yes yes 35 -1 Likely related to alpha-proteobacteria. Synpcc7942_1854 precorrin-3 methyltransferase yes 26 -2 N-J Closely related to alpha proteobacteria. Synpcc7942_1855 cobyrinic acid a,c-diamide synthase yes yes...”
PAAG_00047 hypothetical protein from Paracoccidioides lutzii Pb01
26% identity, 31% coverage
- Transcriptional and proteomic responses to carbon starvation in Paracoccidioides
Lima, PLoS neglected tropical diseases 2014 - “...metabolism PAAG_07529 orotidine 5-phosphate decarboxylase * pyrimidine biosynthesis PAAG_01437 uricase * purine metabolism Secundary metabolism PAAG_00047 siroheme synthase * cobalamin (vitamin B12) biosynthesis PAAG_01519 inositol monophosphatase * secondary metabolism ENERGY Glycolysis and gluconeogenesis PAAG_01015 hexokinase * glycolysis Electron transport and membrane-associated energy conservation PAAG_07285 vacuolar ATP...”
CpC231_1021 precorrin-3B C(17)-methyltransferase from Corynebacterium pseudotuberculosis C231
D9QAC2 Precorrin-3B C(17)-methyltransferase from Corynebacterium pseudotuberculosis (strain C231)
31% identity, 29% coverage
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...cobH CpC231_1020 Precorrin-8X methyl mutase 0.111 1.440 * 1.324 Coenzyme transport and metabolism D9QAC2 cobJ CpC231_1021 Precorrin-3B C(17)-methyltransferase 0.071 1.677 * 1.679 * Coenzyme transport and metabolism D9QAD2 pafB CpC231_1031 Protein pafB 0.927 * 0.125 Transcription D9QAE2 aspA CpC231_1041 Aspartate ammonia-lyase 0.835 * 1.018 1.664 *...”
- “...increased expression in the field isolate is encoded in the gene region from CpC231_1019 to CpC231_1021. All of these have been predicted to reside in a shared operon ( http://www.coryneregnet.de ) and are involved with Precorrin. These genes have homologs in the cob gene products of...”
- Changes in protein abundance are observed in bacterial isolates from a natural host
Rees, Frontiers in cellular and infection microbiology 2015 - “...metabolism D9QAC1 cobH CpC231_1020 Precorrin-8X methyl mutase 0.111 1.440 * 1.324 Coenzyme transport and metabolism D9QAC2 cobJ CpC231_1021 Precorrin-3B C(17)-methyltransferase 0.071 1.677 * 1.679 * Coenzyme transport and metabolism D9QAD2 pafB CpC231_1031 Protein pafB 0.927 * 0.125 Transcription D9QAE2 aspA CpC231_1041 Aspartate ammonia-lyase 0.835 * 1.018...”
2zvbA / Q53WA6 Crystal structure of tt0207 from thermus thermophilus hb8
32% identity, 49% coverage
- Ligand: s-adenosyl-l-homocysteine (2zvbA)
TT_P0013 No description from Thermus thermophilus HB27
32% identity, 49% coverage
CNF00750 hypothetical protein from Cryptococcus neoformans var. neoformans JEC21
28% identity, 37% coverage
Glov_3652 precorrin-2 C20-methyltransferase from Geobacter lovleyi SZ
32% identity, 29% coverage
- Genomic determinants of organohalide-respiration in Geobacter lovleyi, an unusual member of the Geobacteraceae
Wagner, BMC genomics 2012 - “...Cobalamin biosynthesis protein 31(49) Glov_3650 cbiF Precorrin-4 C11-methyltransferase 50(68) Glov_3651 cbiD Cobalamin biosynthesis protein 35(51) Glov_3652 cbiL Precorrin-2 C20-methyltransferase 33(52) Glov_3653 cbiK Anaerobic cobalt chelatase 32(45) Glov_3654 cbiC Precorrin-8X methylmutase 34(52) Glov_3655 cbiA Cobyrinic acid a,c-diamide synthase 39(55) Glov_3656 sirA/cysG Siroheme synthase/ uroporphyrin-III C-methyltransferase 41(57)/40(57) Glov_2767...”
DVU3170 precorrin-3b C17-methyltransferase from Desulfovibrio vulgaris Hildenborough
33% identity, 32% coverage
SG2052 precorrin-3 C17-methyltransferase from Salmonella enterica subsp. enterica serovar Gallinarum str. 287/91
26% identity, 49% coverage
cbiH / Q05590 cobalt-precorrin-3 (C17)-methyltransferase from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
CBIH_SALTY / Q05590 Probable cobalt-factor III C(17)-methyltransferase; Cobalt-precorrin-3 methyltransferase; Cobalt-precorrin-3 methylase; EC 2.1.1.- from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
STM14_2515 precorrin-3B C(17)-methyltransferase from Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S
STM2027 synthesis of vitamin B12 adenosyl cobalamide precursor from Salmonella typhimurium LT2
26% identity, 49% coverage
- function: Methyltransferase that likely catalyzes the ring contraction and methylation of C-17 in cobalt-factor III to form cobalt-factor IV. May also convert cobalt-precorrin-3 to cobalt-precorrin-4.
catalytic activity: Co(II)-factor III + S-adenosyl-L-methionine + H(+) = Co(II)- factor IV + S-adenosyl-L-homocysteine (RHEA:45852) - Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...STM14_2313 Hypothetical protein STM14_2383 STM1963 amyA Cytoplasmic alpha-amylase STM14_2395 STM1974 fliK Flagellar hook-length control protein STM14_2515 STM2027 cbiH Precorrin-3B C17-methyltransferase STM14_2658 STM2155 metG Methionyl-tRNA synthetase STM14_2864 STM2323 nuoG NADH dehydrogenase subunit G STM14_2938 STM2389 fadI 3-ketoacyl-CoA thiolase STM14_2999 STM2441 cysA Sulfate/thiosulfate transporter subunit STM14_3031 STM2472 maeB...”
- Characterisation of Indian gut microbiome for B-vitamin production and its comparison with Chinese cohort
Chandel, The British journal of nutrition 2024 - “...from B 7 pathway (UniProt id P0A6E9) and two genes from B 12 (UniProt id Q05590 and P31570), which could be identified only through Reciprocal Best BLAST approach. The median value of normalised abundances of all genes of a pathway of a given sample was used...”
- Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea.
Pfeiffer, Genes 2021 - “...to N-term of O87689 which has a C-term extension 3b HVO_B0057 (cont.) no 40% STM2027 Q05590 [ 171 ] [ 172 ] 9331403 16198574 equivalent reaction on cobalt-free substrate 3b HVO_B0058 cbiH1 special 32% - O87689 [ 160 ] 23922391 corresponds to N-term of O87689 which...”
- “...a C-term extension; more distant to O87689 than CbiH2 3b HVO_B0058 (cont.) no 30% STM2027 Q05590 [ 171 ] [ 172 ] 9331403 16198574 equivalent reaction on cobalt-free substrate 3b HVO_B0060 cbiF no 40% STM2029 P0A2G9 [ 170 ] [ 173 ] 1451790 16866557 equivalent reaction...”
- Inorganic Polyphosphate Is Essential for Salmonella Typhimurium Virulence and Survival in Dictyostelium discoideum
Varas, Frontiers in cellular and infection microbiology 2018 - “...Hypothetical protein STM14_2383 STM1963 amyA Cytoplasmic alpha-amylase STM14_2395 STM1974 fliK Flagellar hook-length control protein STM14_2515 STM2027 cbiH Precorrin-3B C17-methyltransferase STM14_2658 STM2155 metG Methionyl-tRNA synthetase STM14_2864 STM2323 nuoG NADH dehydrogenase subunit G STM14_2938 STM2389 fadI 3-ketoacyl-CoA thiolase STM14_2999 STM2441 cysA Sulfate/thiosulfate transporter subunit STM14_3031 STM2472 maeB Malic...”
- Salmonella serovar identification using PCR-based detection of gene presence and absence
Arrach, Journal of clinical microbiology 2008 - “...STM1619, STM1867, STM1897, STM1998, STM2005, STM2018, STM2027, STM2134, STM2176, STM2225, STM2237, STM2452, STM2455, STM2456, STM2508, STM2610, STM2618,...”
MMAR_3046 bifunctional protein: CobI-CobJ fusion protein from Mycobacterium marinum M
32% identity, 25% coverage
BMEI0712 bifunctional cbiH protein and precorrin-3B C17-methyltransferase from Brucella melitensis 16M
30% identity, 23% coverage
A5717_31645 precorrin-2 C(20)-methyltransferase from Mycolicibacterium porcinum
32% identity, 26% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MKAN_RS01775 CDN37_RS01855 MPHLCCUG_RS13200 A5717_31635 MXEN_01317 AWC27_RS14575 MSMEG_3871 cobH Cobalt-precorrin-8methylmutase A5717_31640 MXEN_01322 MSMEG_3872 cobIJ Cobalt-precorrin-2 C20-methyltransferase A5717_31645 MXEN_01327 MSMEG_3873 cobK Cobalt-precorrin-6reductase A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292...”
- “...Precorrin-6Y C(5,15)-methyltransferase CDN37_RS01900 A5717_31675 MXEN_01352 AWC27_RS14545 cbiF Precorrin-4 C(11)-methyltransferase A5717_31670 MXEN_01347 cbiG cbiH ATP-binding protein A5717_31645 cbiJ Cobalt-precorrin-6A reductase A5717_31665 MXEN_01342 cbiK cbiL ATP-binding protein A5717_31645 cbiP cbiT Precorrin-6Y-methylase A5717_31675 MXEN_01352 cbiX Sirohydrochlorin ferrochelatase - A5717_10190 SAMEA4434518_00227 MXEN_11286 Urpoporfirynogen III pathway cysG Multifunctional uroporphyrin-III C-methyltransferase/precorrin-2 oxidase/ferrochelatase...”
cbiH60 / O87689 cobalt-cofactor III methyltransferase (EC 2.1.1.272) from Priestia megaterium (see paper)
CBIH_PRIMG / O87689 Cobalt-factor III methyltransferase; Cobalt-precorrin-3 methylase; EC 2.1.1.272 from Priestia megaterium (Bacillus megaterium) (see paper)
O87689 cobalt-factor III methyltransferase (EC 2.1.1.272) from Priestia megaterium (see paper)
25% identity, 43% coverage
- function: Methyltransferase that catalyzes the reduction, ring contraction and methylation of C-17 in cobalt-factor III to form cobalt-precorrin-4. Is also able to convert cobalt-precorrin-3 to cobalt-precorrin-4.
catalytic activity: Co(II)-factor III + AH2 + S-adenosyl-L-methionine = Co- precorrin-4 + A + S-adenosyl-L-homocysteine (RHEA:36155)
cofactor: [4Fe-4S] cluster (Binds 1 [4Fe-4S] cluster.) - Open Issues for Protein Function Assignment in Haloferax volcanii and Other Halophilic Archaea.
Pfeiffer, Genes 2021 - “...[ 170 ] 1451790 equivalent reaction on cobalt-free substrate 3b HVO_B0057 cbiH2 yes 45% - O87689 [ 160 ] 23922391 corresponds to N-term of O87689 which has a C-term extension 3b HVO_B0057 (cont.) no 40% STM2027 Q05590 [ 171 ] [ 172 ] 9331403 16198574 equivalent...”
- “...3b HVO_B0058 cbiH1 special 32% - O87689 [ 160 ] 23922391 corresponds to N-term of O87689 which has a C-term extension; more distant to O87689 than CbiH2 3b HVO_B0058 (cont.) no 30% STM2027 Q05590 [ 171 ] [ 172 ] 9331403 16198574 equivalent reaction on cobalt-free...”
LM6179_1448 bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase from Listeria monocytogenes 6179
29% identity, 31% coverage
WP_013083418 precorrin-3B C(17)-methyltransferase from Priestia megaterium
25% identity, 43% coverage
O27402 cobalt-factor II C20-methyltransferase (EC 2.1.1.151) from Methanothermobacter thermautotrophicus (see paper)
30% identity, 29% coverage
2qbuA / O27402 Crystal structure of methanothermobacter thermautotrophicus cbil (see paper)
30% identity, 29% coverage
- Ligand: s-adenosyl-l-homocysteine (2qbuA)
HVO_B0057 precorrin-3B C17-methyltransferase from Haloferax volcanii DS2
D4GP59 Cobalt-factor-III C17-methyltransferase from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
26% identity, 49% coverage
Rv2066 Probable bifunctional protein, CobI-CobJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase from Mycobacterium tuberculosis H37Rv
33% identity, 27% coverage
- Genome-wide screen identifies host loci that modulate Mycobacterium tuberculosis fitness in immunodivergent mice
Meade, G3 (Bethesda, Md.) 2023 - “...145.321 3.92 144.448 145.321 2.93E02 hp065 Rv0538 Rv0538 8 11.279 4.57 11.279 13.680 1.47E02 hp066 Rv2066 cobI 8 14.819 4.31 12.880 15.343 4.00E02 hp067 Rv1039c PPE15 8 16.687 4.55 13.680 16.946 1.31E02 hp068 Rv3855 ethR 8 87.897 4.62 87.219 89.367 1.44E02 hp069 Rv2161c Rv2161c 8 113.708...”
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MAB_2200c MCNS_30550 XA26_35740 AWC07_17760 H0P51_RS16405 EET03_RS14985 cobH Cobalt-precorrin-8methylmutase Rv2065 MAB_2199c MCNS_30560 AWC07_17765 cobIJ Cobalt-precorrin-2 C20-methyltransferase Rv2066 MCNS_30570 AWC07_17770 cobK Cobalt-precorrin-6reductase Rv2070c MAB_2197 MCNS_30570 AWC07_17795 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c MAB_2195 MCNS_30630 AWC07_17805 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c MAB_2196 MCNS_30620 XA26_35820 AWC07_17800 H0P51_RS16430 EET03_RS15035 cobN Cobalt chelatase Rv2062c MAB_2201...”
- “...26.14527236 133.5466667 188.8635072 cobH Cobalt-precorrin-8methylmutase Rv2065 178.23 4.38254112 0.593333333 0.839100047 0 0 cobIJ Cobalt-precorrin-2 C20-methyltransferase Rv2066 150.18 9.995749096 74.32666667 8.916786915 72.98 58.51295412 cobK Cobalt-precorrin-6reductase Rv2070c 89.46666667 8.680419857 42.11333333 30.69800681 419.98 124.7825583 cobL Cobalt-precorrin-6y C5-methyltransferase Rv2072c 51.73666667 2.388616522 56.36333333 44.1996458 219.44 310.3350241 cobM Cobalt-precorrin-4 C11-methyltransferase Rv2071c 32.80333333...”
- Implementation of homology based and non-homology based computational methods for the identification and annotation of orphan enzymes: using Mycobacterium tuberculosis H37Rv as a case study
Sinha, BMC bioinformatics 2020 - “...synthetase) Rv2157c 6.3.1.2 (Glutamine synthetase) Rv2222c 1.14.13.83 (Precorrin-3B synthase/porphyrin and chlorophyll metabolism) 2.1.1.131 (Precorrin-3B C(17)-methyltransferase) Rv2066 2.1.1.130 (Precorrin-2C (20)-methyltransferase) Rv2066 1.3.3.3 (Coprogen oxidase/porphyrin and chlorophyll metabolism) 1.3.3.4 (Protoporphyrinogen oxidase) Rv2677c 4.1.1.37 (Uroporphyrinogen decarboxylase) Rv2678c 3.6.1.40 (Guanosine-5-triphosphate, 3diphosphate diphosphatase/purine metabolism) 3.1.7.2 (Guanosine-3,5-bis(diphosphate) 3-diphosphatase) Rv2583c 2.7.6.5 (GTP diphosphokinase)...”
- Purifying Selective Pressure Suggests the Functionality of a Vitamin B12 Biosynthesis Pathway in a Global Population of Mycobacterium tuberculosis
Minias, Genome biology and evolution 2018 - “...pduO Rv1314c 582 3.61 1.20 3 2 cobT Rv2207 1,086 3.04 1.07 5 5 cobIJ Rv2066 1,527 3.73 1.00 12 12 hemY Rv2850c 1,833 4.31 0.97 12 9 cobD Rv2236c 942 3.29 0.94 3 2 cobN Rv2062c 3,585 3.40 0.93 23 21 cobS Rv2208 750 4.13...”
- iniBAC induction Is Vitamin B12- and MutAB-dependent in Mycobacterium marinum
Boot, The Journal of biological chemistry 2016 - “...Rv3208 Rv2940c Rv2848 Rv1492 Rv1493 Rv1496 Rv2062c Rv2065 Rv2066 Rv2070c Rv2071c Rv2072c Rv0254c Rv2207 Rv2231c Rv2236c Rv1013 none Rv0819 3153 684 5616 1377...”
- Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families and their association with the duplication of the ESAT-6 (esx) gene cluster regions
Gey, BMC evolutionary biology 2006 - “...M. tuberculosis most homologous to PPE Rv0280 NT03MA1895 MAP1813c Absent in M. tuberculosis Situated between Rv2066 and Rv2069 most homologous to PPE Rv0256c NT03MA2236 MAP2136c Situated between Rv2348 and Rv2357c, in other words in the position of PPE Rv2352, Rv2353 or Rv2356, but does not contain...”
CAETHG_1114 precorrin-3B C(17)-methyltransferase from Clostridium autoethanogenum DSM 10061
26% identity, 50% coverage
Synpcc7942_1854 precorrin-3B C17-methyltransferase from Synechococcus elongatus PCC 7942
29% identity, 32% coverage
- Blueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942
Delaye, BMC genomics 2011 - “...Synpcc7942_1851 ferredoxin--nitrite reductase Nitrogen metabolism Synpcc7942_1852 precorrin-8X methylmutase Chlorophyll metabolism Synpcc7942_1853 precorrin-2 C20-methyltransferase Chlorophyll metabolism Synpcc7942_1854 precorrin-3 methyltransferase Chlorophyll metabolism Synpcc7942_1855 cobyrinic acid a,c-diamide synthase Chlorophyll metabolism Synpcc7942_2151 cellulose synthase (UDP-forming) Starch and sucrose metabolism Synpcc7942_2238 glucose transport protein Transport Synpcc7942_2436 peptide methionine sulfoxide reductase Repair...”
- “...transferred among Cyanobacteria. Synpcc7942_1853 cobalt-factor II C20-methyltransferase yes yes 35 -1 Likely related to alpha-proteobacteria. Synpcc7942_1854 precorrin-3 methyltransferase yes 26 -2 N-J Closely related to alpha proteobacteria. Synpcc7942_1855 cobyrinic acid a,c-diamide synthase yes yes 34 -2 Likely transferred among cyanobacteria. Synpcc7942_1873 two component transcriptional regulator 35...”
DCF50_RS14480, UNSWDHB_RS14695 precorrin-3B C(17)-methyltransferase from Dehalobacter sp. CF
31% identity, 34% coverage
MSMEG_3873 precorrin-2 C(20)-methyltransferase from Mycolicibacterium smegmatis MC2 155
MSMEG_3873 cobalamin biosynthesis protein cobIJ from Mycobacterium smegmatis str. MC2 155
31% identity, 31% coverage
- Cobalamin is present in cells of non-tuberculous mycobacteria, but not in Mycobacterium tuberculosis
Minias, Scientific reports 2021 - “...MPHLCCUG_RS13200 A5717_31635 MXEN_01317 AWC27_RS14575 MSMEG_3871 cobH Cobalt-precorrin-8methylmutase A5717_31640 MXEN_01322 MSMEG_3872 cobIJ Cobalt-precorrin-2 C20-methyltransferase A5717_31645 MXEN_01327 MSMEG_3873 cobK Cobalt-precorrin-6reductase A5717_31665 MXEN_01342 MSMEG_3875 cobL Cobalt-precorrin-6y C5-methyltransferase A5717_31675 MXEN_01352 MSMEG_3878 cobM Cobalt-precorrin-4 C11-methyltransferase MKAN_RS01815 CDN37_RS01895 MPHLCCUG_RS13225 A5717_31670 MXEN_01347 MSMEG_3877 cobN Cobalt chelatase MKAN_RS01760 CDN37_RS01840 MPHLCCUG_RS12530 A5717_31630 MXEN_01292 AWC27_RS14585 MSMEG_3864...”
- “...10.51069138 MSMEG_3871 43.05333333 9.251671921 cobH Cobalt-precorrin-8methylmutase MAB_2199c 53.07 1.728670009 MSMEG_3872 110.2366667 37.68649802 cobIJ Cobalt-precorrin-2 C20-methyltransferase MSMEG_3873 52.53333333 35.62759651 cobK Cobalt-precorrin-6reductase MAB_2197 118.63 8.898027871 MSMEG_3875 40.99 6.827935266 cobL Cobalt-precorrin-6y C5-methyltransferase MAB_2195 95.33 10.65838168 MSMEG_3878 33.24333333 4.282117856 cobM Cobalt-precorrin-4 C11-methyltransferase MAB_2196 190.0066667 34.70278423 MSMEG_3877 40.97333333 4.718308313 cobN Cobalt...”
- Key Transitions in the Evolution of Rapid and Slow Growing <i>Mycobacteria</i> Identified by Comparative Genomics
Bachmann, Frontiers in microbiology 2019 - “...ViuB COG2375 Siderophore-interacting protein MSMEG_3035 Efp COG0231 Translation elongation factor P MSMEG_3059 Aes COG0657 Esterase/lipase MSMEG_3873 COG4178 ABC-type uncharacterized transport system MSMEG_3873 CobJ COG1010 Precorrin-3B methylase MSMEG_4286 LipA COG0320 Lipoate synthase MSMEG_4329 COG4799 Acetyl-CoA carboxylase, carboxyltransferase component MSMEG_4342 GloB COG0491 Zn-dependent hydrolases, including glyoxylases MSMEG_4484 COG1512...”
- Methylfolate Trap Promotes Bacterial Thymineless Death by Sulfa Drugs
Guzzo, PLoS pathogens 2016 - “...of B 12 -dependent methionine synthase. Two other mutants had insertions at TA 112-113 of msmeg_3873 , which encodes an enzyme (CobIJ) that catalyzes two methylation steps, precorrin-2 C20 methyltransferase [CobI, EC:2.1.1.130] and precorrin-3B C17-methyltransferase [CobJ, EC:2.1.1.131], of the B 12 (cobalamin) biosynthetic pathway. Interestingly, the...”
- “...SULFA resistance, and 5-CH 3 -H 4 PteGlu n metabolism, the encoding genes, msmeg_4185 and msmeg_3873 , respectively, were individually deleted by homologous recombination [ 34 ]. Similar to the transposon mutants, the targeted null mutants, Ms metH and Ms cobIJ , displayed increased SULFA susceptibility...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory