PaperBLAST
PaperBLAST Hits for sp|Q9HUI9|ARUH_PSEAE Arginine--pyruvate transaminase AruH OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=aruH PE=1 SV=1 (393 a.a., MRYSDFTQRI...)
Show query sequence
>sp|Q9HUI9|ARUH_PSEAE Arginine--pyruvate transaminase AruH OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=aruH PE=1 SV=1
MRYSDFTQRIAGDGAAAWDIHYRALARVEQGEEILLLSVGDPDFDTPAPIVQAAIDSLLA
GNTHYADVRGKRALRQRIAERHRRRSGQAVDAEQVVVLAGAQCALYAVVQCLLNPGDEVI
VAEPMYVTYEAVFGACGARVVPVPVRSENGFRVQAEEVAALITPRTRAMALNSPHNPSGA
SLPRATWEALAELCMAHDLWMISDEVYSELLFDGEHVSPASLPGMADRTATLNSLSKSHA
MTGWRVGWVVGPAALCAHLENLALCMLYGSPEFIQDAACTALEAPLPELEAMREAYRRRR
DLVIECLADSPGLRPLRPDGGMFVMVDIRPTGLSAQAFADRLLDRHGVSVLAGEAFGPSA
AGHIRLGLVLGAEPLREACRRIALCAAELLGQA
Running BLASTp...
Found 250 similar proteins in the literature:
aruH / Q9HUI9 L-arginine:pyruvate transaminase subunit (EC 2.6.1.84) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
ARUH_PSEAE / Q9HUI9 Arginine--pyruvate transaminase AruH; EC 2.6.1.84 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
Q9HUI9 arginine-pyruvate transaminase (EC 2.6.1.84) from Pseudomonas aeruginosa (see paper)
PA4976 aspartate transaminase from Pseudomonas aeruginosa PAO1
100% identity, 100% coverage
- function: Catalyzes the conversion of L-arginine into 2-ketoarginine via transamination. L-arginine is the best substrate, but it can also use L-lysine, L-methionine, L-leucine, ornithine and L-glutamine, which indicates that it may have a broader physiological function in amino acid catabolism.
catalytic activity: L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L- alanine (RHEA:13833)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - A systems biology approach to drug targets in Pseudomonas aeruginosa biofilm
Sigurdsson, PloS one 2012 - “...(P4810), FdnH (PA4811), FdnG (PA4812) Amino acid synthesis LysC (PA0904), Hom (PA3736), Ldh (PA3418), AruH (PA4976), GdhA (PA4588) Central metabolism Fda (PA0555), SdhC (PA1581), SdhD (PA1582), SdhA (PA1583), SdhB (PA1584), Fbp (PA5110), TktA (PA0548), AceA (PA2634), Edd (PA3194), GapA (PA3195) Energy metabolism PetC(PA4429), Ppa (PA4031) Ethanol/...”
- L-lysine catabolism is controlled by L-arginine and ArgR in Pseudomonas aeruginosa PAO1
Chou, Journal of bacteriology 2010 - “...expression from pUCP18. Full-length ldcA (PA1818) and aruIH (PA4976 and PA4977) genes were successfully PCR amplified and subcloned into pUCP18 to construct...”
- Arginine racemization by coupled catabolic and anabolic dehydrogenases
Li, Proceedings of the National Academy of Sciences of the United States of America 2009 - “...standard errors of less than 5%. PA0265 PA0266 PA1421 PA4976 PA4977 PA5312 Group IV: Arginine and glutamate biosynthesis gabD gabT gbuA aruH aruI kauB 796 943...”
- Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa
Yang, Journal of bacteriology 2007 - “...LB plates supplemented with tetracycline and streptomycin. PA4975, PA4976 (aruH), and PA5304 (dadA) knockout mutants were also constructed by a strategy similar...”
- “...PCR products: for PA4975 and PA4976 (aruH), 5-GTCTAAGCTTGACTGGCCTGGCGCGCGTCG-3 and 5-CGCA AGCTTCGGGCAGTCCGGCGTGACCCT-3; for PA5304 (dadA), 5-ACGG...”
PP_3721 arginine:pyruvate transaminase AruH (EC 2.6.1.84) from Pseudomonas putida KT2440
PP_3721 aspartate aminotransferase from Pseudomonas putida KT2440
81% identity, 98% coverage
SMc02262 PUTATIVE AMINOTRANSFERASE PROTEIN from Sinorhizobium meliloti 1021
40% identity, 97% coverage
TTC1960 No description from Thermus thermophilus HB27
40% identity, 93% coverage
AAPAT_THET8 / Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 5 papers)
Q56232 aspartate-prephenate aminotransferase (EC 2.6.1.78) from Thermus thermophilus (see paper)
aspC / RF|YP_143312.1 aspartate transaminase; EC 2.6.1.1 from Thermus thermophilus HB8 (see 2 papers)
TTHA0046 aspartate aminotransferase from Thermus thermophilus HB8
40% identity, 93% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:25070637, PubMed:8907187). Can also transaminate prephenate in the presence of aspartate (PubMed:25070637, PubMed:30771275).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + oxaloacetate = prephenate + L-aspartate (RHEA:20445)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Lysine propionylation is a prevalent post-translational modification in Thermus thermophilus
Okanishi, Molecular & cellular proteomics : MCP 2014 - “...TTHA1965 TTHB240 Amino acid transport and metabolism TTHA0046 TTHA0216 TTHA0457 TTHA0545 TTHA0582 TTHA0722 TTHA1210 TTHA1211 TTHA1212 TTHA1496 Metabolism Energy...”
1b5oA / Q56232 Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
40% identity, 93% coverage
- Ligand: pyridoxal-5'-phosphate (1b5oA)
CAA67877.1 aspartate aminotransferase from Thermus aquaticus (see paper)
O33822 Probable aspartate/prephenate aminotransferase from Thermus aquaticus
38% identity, 96% coverage
- PIKFYVE inhibition mitigates disease in models of diverse forms of ALS.
Hung, Cell 2023 - “...17. For treatment with apilimod, cultures were treated with DMSO or 3 M apilimod (Achemblock, O33822) after neurotrophic factor withdrawal (starting at day 17). For treatment with GW4869 to block exocytosis, cultures were treated with 10 M GW4869 (Cayman Chemical, 13127) along with DMSO or 3...”
- “...Lenti-X concentrator TaKaRa 631232 Polybrene (Hexadimethrine bromide) Sigma H9268-5G Neurobasal-A Life Technologies 10888-022 Apilimod achemblock O33822 GW 4869 (hydrochloride hydrate) Cayman Chemicals 13127 SB431542 Cayman Chemical 13031 CHIR99021 Cayman Chemical 13122 Retinoic Acid Millipore Sigma R2625 DMH1 selleckchem S7146 Purmorphamine Cayman Chemical 10009634 Donor Equine Serum...”
- ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids.
Bowles, Cell 2021 - “...acids 100X Thermo 11140050 Penicillin-streptomycin 50X Corning 30-001-CI (+)-MK-801 (hydrogen maleate) Cayman 10009019 Apilimod AChemBlock O33822 CNQX Cayman 14618 Dorsomorphin Tocris 3093 Dorsomorphin Cayman 11967 L-Glutamic acid monosodium salt hydrate Millipore G1626 Nimodipine Cayman 14573 SB-431542 Tocris 1614 SB-431542 Cayman 13031 XAV-939 Tocris 3748 Y-27632 Tocris...”
BL0628 similar to aspartate aminotransferase from Bifidobacterium longum NCC2705
40% identity, 80% coverage
BBIF_1610 pyridoxal phosphate-dependent aminotransferase from Bifidobacterium bifidum S17
38% identity, 88% coverage
- Proteomic Profiling of Bifidobacterium bifidum S17 Cultivated Under In Vitro Conditions
Wei, Frontiers in microbiology 2016 - “...aminotransferase 394 43.5 BBIF_1428 serC Phosphoserine aminotransferase 380 40.6 BBIF_1519 bbif_1519 N-succinyldiaminopimelate aminotransferase 392 41.5 BBIF_1610 aspC2 Aspartate aminotransferase 409 44.5 a aa, amino acids. b kDa, kilo Dalton. FIGURE 2 Enzymes of the bifidus shunt (A) and Embden-Meyerhof pathway (B) detected in the proteome of...”
PF1253 aspartate transaminase from Pyrococcus furiosus DSM 3638
37% identity, 91% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...recognized were Tyr, Phe, Glu, His, and Trp ( Matsui et al., 2000 ). The PF1253 protein from P. furiosus , referred to as AroAT II, displayed preference to Phe, Tyr and Trp, with highest k cat / K m toward Phe ( Ward et al.,...”
- “...also displays activity toward His ( Matsui et al., 2000 ). The activities of the PF1253 ( Ward et al., 2002 ) and OCC_04737 ( Andreotti et al., 1994 ) proteins toward His have not been examined. In the case of the TK2268 protein, it is...”
- Genome sequencing of a genetically tractable Pyrococcus furiosus strain reveals a highly dynamic genome
Bridger, Journal of bacteriology 2012 - “...region (6,238 bp) that includes the genes PF1249 to PF1253 (deleted) and PF1254 (904 bp deleted at the 5= end). These encode an ABC transporter, a...”
- “...(bp) PF0073 PF1249d PF1250 PF1251 PF1252 PF1253 PF1254 Beta-glucosidase (CelB) ABC transporter Hypothetical protein Alpha-galactosidase, NEW3 domain...”
- Whole-genome DNA microarray analysis of a hyperthermophile and an archaeon: Pyrococcus furiosus grown on carbohydrates or peptides
Schut, Journal of bacteriology 2003 - “...PF0974 PF1057 [Amino acid metabolism] PF1245 PF1246 PF1253 PF1341 2-Keto acid ferredoxin oxidoreductases PF1767 PF1768 PF1769 PF1770 PF1771 PF1772 PF1773 PF1874...”
- “...up-regulated. They include one yet to be characterized, PF1253 (6.1-fold) and two that have been purified, PF0121 (4.5-fold) (3) and PF1497 (2.5-fold) (62)....”
- Molecular analysis of the role of two aromatic aminotransferases and a broad-specificity aspartate aminotransferase in the aromatic amino acid metabolism of Pyrococcus furiosus
Ward, Archaea (Vancouver, B.C.) 2002 - “...furiosus genome (http://www.genome.utah.edu) revealed two putative paralogs (PF1253 and PF1702). To study their function, the latter genes were cloned,...”
OCC_08839 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Thermococcus litoralis DSM 5473
35% identity, 99% coverage
1gd9A / O59096 Crystall structure of pyrococcus protein-a1 (see paper)
37% identity, 91% coverage
- Ligand: pyridoxal-5'-phosphate (1gd9A)
O59096 aromatic-amino-acid transaminase (EC 2.6.1.57) from Pyrococcus horikoshii (see paper)
PH1371 aspartate aminotransferase from Pyrococcus horikoshii OT3
37% identity, 91% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...G6 have been relatively well studied. In the case of G1, the structure of the PH1371 protein from P. horikoshii has been elucidated, and the amino donors most recognized were Tyr, Phe, Glu, His, and Trp ( Matsui et al., 2000 ). The PF1253 protein from...”
- “...with other members of G1. To a lower degree, the protein also recognized His. The PH1371 protein also displays activity toward His ( Matsui et al., 2000 ). The activities of the PF1253 ( Ward et al., 2002 ) and OCC_04737 ( Andreotti et al., 1994...”
- Molecular analysis of the role of two aromatic aminotransferases and a broad-specificity aspartate aminotransferase in the aromatic amino acid metabolism of Pyrococcus furiosus
Ward, Archaea (Vancouver, B.C.) 2002 - “...abyssi (PAB2227 and PAB0525) and P. horikoshii (PH0207 and PH1371). The structural tyrC gene encoding the AroAT II consists of 1203 bp and encodes a protein of...”
TK0548 aromatic aminotransferase from Thermococcus kodakaraensis KOD1
37% identity, 91% coverage
- TK2268 encodes the major aminotransferase involved in the conversion from oxaloacetic acid to aspartic acid in <i>Thermococcus kodakarensis</i>
Su, Applied and environmental microbiology 2025 (PubMed)- “...of aspartate is unknown. Here, four Class I aminotransferases in T. kodakarensis encoded by TK0186, TK0548, TK1094, and TK2268 were examined to identify the enzyme(s) responsible for the conversion of oxaloacetate to aspartate. Among the four proteins, the TK2268 protein (TK2268p) was the only protein to...”
- Removal of phosphoglycolate in hyperthermophilic archaea
Michimori, Proceedings of the National Academy of Sciences of the United States of America 2024 - “...or microaerobic conditions, respectively. ( D ) AGAT activity in cell extracts from KU216, TK0186, TK0548, TK2268, and TK1094 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. Activity measurements were carried out as described...”
- “...T. kodakarensis proteome resulted in the identification of multiple proteins (encoded by TK0186, TK2268, TK1094, TK0548, TK0260, and TK0250) with varying degrees of homology, suggesting that multiple proteins might exhibit aminotransferase activity with glyoxylate as the amino acceptor. Among the six candidate genes, TK0250 and TK0260...”
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...Here we examined the biochemical properties and physiological roles of two Class I aminotransferases. The TK0548 protein was produced in Escherichia coli and the TK2268 protein in T. kodakarensis . Purified TK0548 protein preferred Phe, Trp, Tyr, and His, and to a lower extent, Leu, Met...”
- “...Cys, Leu, Ala, Met and Tyr. Both proteins recognized 2-oxoglutarate as the amino acceptor. The TK0548 protein exhibited the highest k cat / K m value toward Phe, followed by Trp, Tyr, and His. The TK2268 protein exhibited highest k cat / K m values for...”
- Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles
Okada, Journal of bacteriology 2014 - “...instrument after analysis by the MASCOT database. TK0545, TK0548, TK0967, and TK1691 were identified as candidate enzymes, and the corresponding genes were...”
- “...the T. kodakarensis genome: TK0545, bp 465352 to 466569 (); TK0548, bp 467635 to 468804 (); TK0967, bp 844599 to 845645 (); and TK1691, bp 1488064 to 1489119...”
PAB0525 aspartate aminotransferase from Pyrococcus abyssi GE5
36% identity, 89% coverage
YP_001737635 aspartate/tyrosine/aromatic aminotransferase from Candidatus Korarchaeum cryptofilum OPF8
35% identity, 92% coverage
MA1819 aspartate aminotransferase from Methanosarcina acetivorans C2A
34% identity, 90% coverage
- Deconstructing Methanosarcina acetivorans into an acetogenic archaeon
Schöne, Proceedings of the National Academy of Sciences of the United States of America 2022 - “...responsible for MKOmtrSFs phenotype. Unique to MKOmtrSF is a deletion of a threonine codon in MA1819, which encodes a homolog of AspB ( 25 ). Strikingly, MKOmtrSF also carries a 177,114-base pair lesion (compared to the reference strains), which corresponds to approximately 3% of its genome....”
- “...in the region missing, SI Appendix , Table S5 ), or the codon deletion in MA1819 ( SI Appendix , Table S4 ) caused the phenotype of MKOmtrSF could not be unraveled, if only for the sheer number of genes affected. To learn how the genomic...”
AAPAT_CERS1 / A3PMF8 Aspartate/prephenate aminotransferase; AspAT / PAT; EC 2.6.1.1; EC 2.6.1.79 from Cereibacter sphaeroides (strain ATCC 17029 / ATH 2.4.9) (Rhodobacter sphaeroides) (see paper)
39% identity, 89% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:24302739). Can also transaminate prephenate in the presence of glutamate (PubMed:24302739).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + 2-oxoglutarate = prephenate + L-glutamate (RHEA:22880)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
TON_0565 aromatic aminotransferase from Thermococcus onnurineus NA1
36% identity, 92% coverage
AAPAT_RHIME / Q02635 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.79 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see 4 papers)
Q02635 aspartate transaminase (EC 2.6.1.1); aspartate-prephenate aminotransferase (EC 2.6.1.78); glutamate-prephenate aminotransferase (EC 2.6.1.79) from Sinorhizobium meliloti (see 2 papers)
aatA / AAA26245.1 aspartate aminotransferase from Sinorhizobium meliloti (see paper)
SMc01578 ASPARTATE AMINOTRANSFERASE A (TRANSAMINASE) PROTEIN from Sinorhizobium meliloti 1021
WP_010969860 pyridoxal phosphate-dependent aminotransferase from Sinorhizobium meliloti 1021
35% identity, 90% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:24302739, PubMed:8096210). Can also transaminate prephenate in the presence of glutamate (PubMed:24302739). Required for symbiotic nitrogen fixation (PubMed:2019560).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + 2-oxoglutarate = prephenate + L-glutamate (RHEA:22880)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
disruption phenotype: Mutant is unable to grow on aspartate but can grow on other carbon and nitrogen sources. Mutant is unable to fix nitrogen within nodules formed on alfalfa. - Impact of Hydrogen on the Transcriptome of Sinorhizobium meliloti 1021 Using RNA-sequencing Technology
Liu, Polish journal of microbiology 2020 - “...et al. 2015 ). RT-qPCR and physiological research verification . For the specific genes SMc02983, SMc01578, SMc01656, SMc02677, SM_b20752, cyoD, cyoC , and cyoB , real-time quantitative PCR was performed with the following primers. SMc02983 : 5-GGCACAATCGTGCGTGTCCTTCAAC-3 and 5-GCCATTCGCCCATCTCAT-3; SMc01578: 5-GACTAGACTACGGTCCCAAC-3 and 5-TTATTACTCGCTCGTGATCTCATC-3; SMc01656: 5-GCCTTGTGTCTGAGGTTATCATC-3 and...”
- “...in oxidative phosphorylation. SMc02983 encodes arginine decarboxylase, SMc01656 encodes gamma-aminobutyraldehyde dehydrogenase, SM_b20752 encodes 3-hydroxyisobutyryl-CoA hydrolase, SMc01578 encodes aspartate aminotransferase, and SMc02677 ( proC ) encodes pyrroline-5-carboxylate reductase; these genes are involved in arginine and proline metabolism. The transcription levels of SMc02983, SMc01656, SMc01578, SMc02677, SM_b20752, cyoB,...”
- Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms
Graindorge, The Journal of biological chemistry 2014 - “...database under the following UniProt accession numbers: Q02635, A3PMF8, P54691, Q55128, Q55828, Q3AJX2, Q82IK5, Q82DR2, Q82JN7, Q82EU6, O50434, Q82WA8, Q82UJ8,...”
- “...corresponded to an aminotransferase: an aspartate aminotransferase (Q02635) for R. meliloti, a branched-chain aminotransferase (P54691) for Synechocystis, and a...”
- Tyrosine metabolism: identification of a key residue in the acquisition of prephenate aminotransferase activity by 1β aspartate aminotransferase.
Giustini, The FEBS journal 2019 (PubMed)- GeneRIF: X-ray crystal structure analysis supported by site-directed mutagenesis, modeling, and molecular dynamics simulations identified a molecular determinant of prephenate aminotransferase activity in the flexible N-terminal loop of 1beta AAT. A Lys/Arg/Gln residue in position 12 in the sequence present only in PAT-competent enzymes could interact with the 4-hydroxyl group of the prephenate substrate.
6f77A / Q02635 Crystal structure of the prephenate aminotransferase from rhizobium meliloti (see paper)
35% identity, 90% coverage
- Ligand: pyridoxal-5'-phosphate (6f77A)
PF0522 aspartate transaminase from Pyrococcus furiosus DSM 3638
34% identity, 93% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...Pyrococcus furiosus I PF1253 G1 Aromatic aminotransferase Phe, Tyr, Trp 2-OG Ward et al. (2002) PF0522 G2 (Aspartate aminotransferase) PF1497 G3 Alanine aminotransferase Ala, Glu, (Asp, Ile, Leu) 2-OG, Pyr Ward et al. (2000) PF1702 G4 Aspartate aminotransferase Asp, (Glu) 2-OG, (Pyr, phenylpyruvate) Ward et al....”
TK2268 aspartate aminotransferase from Thermococcus kodakaraensis KOD1
34% identity, 93% coverage
- TK2268 encodes the major aminotransferase involved in the conversion from oxaloacetic acid to aspartic acid in <i>Thermococcus kodakarensis</i>
Su, Applied and environmental microbiology 2025 (PubMed)- “...unknown. Here, four Class I aminotransferases in T. kodakarensis encoded by TK0186, TK0548, TK1094, and TK2268 were examined to identify the enzyme(s) responsible for the conversion of oxaloacetate to aspartate. Among the four proteins, the TK2268 protein (TK2268p) was the only protein to recognize oxaloacetate as...”
- Removal of phosphoglycolate in hyperthermophilic archaea
Michimori, Proceedings of the National Academy of Sciences of the United States of America 2024 - “...microaerobic conditions, respectively. ( D ) AGAT activity in cell extracts from KU216, TK0186, TK0548, TK2268, and TK1094 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. Activity measurements were carried out as described in...”
- “...against the T. kodakarensis proteome resulted in the identification of multiple proteins (encoded by TK0186, TK2268, TK1094, TK0548, TK0260, and TK0250) with varying degrees of homology, suggesting that multiple proteins might exhibit aminotransferase activity with glyoxylate as the amino acceptor. Among the six candidate genes, TK0250...”
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...of two Class I aminotransferases. The TK0548 protein was produced in Escherichia coli and the TK2268 protein in T. kodakarensis . Purified TK0548 protein preferred Phe, Trp, Tyr, and His, and to a lower extent, Leu, Met and Glu. The TK2268 protein preferred Glu and Asp,...”
- “...k cat / K m value toward Phe, followed by Trp, Tyr, and His. The TK2268 protein exhibited highest k cat / K m values for Glu and Asp. The TK0548 and TK2268 genes were individually disrupted, and both disruption strains displayed a retardation in growth...”
- An archaeal histone is required for transformation of Thermococcus kodakarensis
Čuboňováa, Journal of bacteriology 2012 - “...TK1431 TK1046 TK1624 TK0560 TK1245 TK2048 TK0136 TK0307 TK2268 TK0352 TK2035 TK1539 TK0878 TK1500 TK0465 TK1534 TK1295 TK0308 TK1469 TK1174 Fold difference...”
Cthe_0363 aminotransferase from Clostridium thermocellum ATCC 27405
37% identity, 90% coverage
BSU31400 hypothetical protein from Bacillus subtilis subsp. subtilis str. 168
34% identity, 96% coverage
PH0771 aspartate aminotransferase from Pyrococcus horikoshii OT3
33% identity, 91% coverage
MOC_2191 pyridoxal phosphate-dependent aminotransferase from Methylobacterium oryzae CBMB20
36% identity, 88% coverage
BC4901 Aromatic amino acid aminotransferase from Bacillus cereus ATCC 14579
36% identity, 88% coverage
BH3350 hypothetical protein from Bacillus halodurans C-125
36% identity, 90% coverage
APA386B_861 pyridoxal phosphate-dependent aminotransferase from Acetobacter pasteurianus 386B
37% identity, 94% coverage
- Genome-Scale Metabolic Reconstruction of Acetobacter pasteurianus 386B, a Candidate Functional Starter Culture for Cocoa Bean Fermentation
Pelicaen, Frontiers in microbiology 2019 - “...different orthogroups. Aspartate aminotransferase (EC 2.6.1.1) was linked to three genes, likely encoding this enzyme (APA386B_861, APA386B_862, and APA386B_942). These were not predicted to be homologous to the E. coli aspartate aminotransferase gene (b0928, aspC ) but shared considerable sequence identity with curated SwissProt sequences from...”
- “...Thus, the L-tyrosine-forming reaction was tentatively associated to the aspartate aminotransferases encoded in the genome (APA386B_861, APA386B_862, and APA386B_942). Since A. pasteurianus 386B was able to grow in a defined medium containing sulfate as the sole sulfur source, sulfate assimilation was assumed ( Figure 3B )....”
AL01_03035 pyridoxal phosphate-dependent aminotransferase from Bombella intestini
36% identity, 91% coverage
BMF77_00644 pyridoxal phosphate-dependent aminotransferase from Dolichospermum sp. UHCC 0315A
35% identity, 90% coverage
- Insight into the genome and brackish water adaptation strategies of toxic and bloom-forming Baltic Sea Dolichospermum sp. UHCC 0315
Teikari, Scientific reports 2019 - “...indicated by the induced expression of BMF77_01290, nitrate reduction (BMF77_3991; 3993), and fine-tuning amino-acid metabolism (BMF77_00644; 02305; 03528). High demand for the reorganization of nitrogen metabolism was also seen among 30 most up and downregulated genes (Fig. 5 ). A general stress response was implemented, hindering...”
- “...branched-chain amino acid transport system permease protein LivH 1,10 BMF77_01290 Putative serine protease HtrA 1,01 BMF77_00644 Aspartate aminotransferase 1,20 BMF77_01666 Cystathionine beta-lyase MetC 1,04 BMF77_01996 Cysteine desulfurase NifS 1,22 BMF77_01034 ATP-dependent Clp protease ATP-binding subunit ClpC 1,08 Transcription and translation BMF77_04949 50S ribosomal protein L16 arginine...”
APA386B_862 pyridoxal phosphate-dependent aminotransferase from Acetobacter pasteurianus 386B
35% identity, 82% coverage
- Genome-Scale Metabolic Reconstruction of Acetobacter pasteurianus 386B, a Candidate Functional Starter Culture for Cocoa Bean Fermentation
Pelicaen, Frontiers in microbiology 2019 - “...orthogroups. Aspartate aminotransferase (EC 2.6.1.1) was linked to three genes, likely encoding this enzyme (APA386B_861, APA386B_862, and APA386B_942). These were not predicted to be homologous to the E. coli aspartate aminotransferase gene (b0928, aspC ) but shared considerable sequence identity with curated SwissProt sequences from other...”
- “...the L-tyrosine-forming reaction was tentatively associated to the aspartate aminotransferases encoded in the genome (APA386B_861, APA386B_862, and APA386B_942). Since A. pasteurianus 386B was able to grow in a defined medium containing sulfate as the sole sulfur source, sulfate assimilation was assumed ( Figure 3B ). Indeed,...”
BAS1454 aspartate aminotransferase from Bacillus anthracis str. Sterne
33% identity, 93% coverage
BC1546 Aspartate aminotransferase from Bacillus cereus ATCC 14579
32% identity, 93% coverage
ARAT2_THELN / H3ZPU1 Aromatic-amino-acid aminotransferase 2; ARAT-II; AROAT; EC 2.6.1.57 from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see paper)
OCC_04737 pyridoxal phosphate-dependent aminotransferase from Thermococcus litoralis DSM 5473
36% identity, 93% coverage
- function: Catalyzes the transamination of phenylalanine, tyrosine and tryptophan. Shows virtually no activity towards aspartic acid, alanine, valine or isoleucine.
catalytic activity: an aromatic L-alpha-amino acid + 2-oxoglutarate = an aromatic oxo-acid + L-glutamate (RHEA:17533)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...highest k cat / K m toward Phe ( Ward et al., 2002 ). The OCC_04737 protein from T. litoralis , referred to as ArAT II, also displayed similar properties, preferring Phe, Tyr, and Trp ( Andreotti et al., 1994 ). In the case of G6,...”
- “...al., 2000 ). The activities of the PF1253 ( Ward et al., 2002 ) and OCC_04737 ( Andreotti et al., 1994 ) proteins toward His have not been examined. In the case of the TK2268 protein, it is the first characterized representative of G2, and displays...”
BMB171_C1377 aspartate transaminase AspB from Bacillus thuringiensis BMB171
32% identity, 93% coverage
Q9RWP3 Aminotransferase from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
36% identity, 92% coverage
6f35A / P58350 Crystal structure of the aspartate aminotranferase from rhizobium meliloti (see paper)
35% identity, 91% coverage
- Ligand: pyridoxal-5'-phosphate (6f35A)
SMc04386 2-aminoadipate:2-oxoglutarate aminotransferase (EC 2.6.1.39) from Sinorhizobium meliloti 1021
AAT_RHIME / P58350 Aspartate aminotransferase; AAT; AspAT; Putative 2-aminoadipate transaminase; Transaminase A; EC 2.6.1.1; EC 2.6.1.39 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) (see 2 papers)
35% identity, 89% coverage
- mutant phenotype: Specifically important for: L-Lysine. This is required for catabolism of lysine on the way to glutarate. The amino group donor is uncertain
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (By similarity). Genetic evidence shows that this protein is involved in L-lysine catabolism. It may have 2-aminoadipate:2-oxoglutarate aminotransferase activity (PubMed:29769716).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L- glutamate (RHEA:12601)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
Q8YMS6 aspartate transaminase (EC 2.6.1.1) from Nostoc sp. (see paper)
alr4853 aspartate aminotransferase from Nostoc sp. PCC 7120
34% identity, 90% coverage
- β-N-Methylamino-L-Alanine (BMAA) Causes Severe Stress in Nostoc sp. PCC 7120 Cells under Diazotrophic Conditions: A Proteomic Study
Koksharova, Toxins 2021 - “...0.0013 *** 47 aspartate aminotransferase [EC:2.6.1.1] 2-oxoglutarate-glutamate aminotransferase L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate alr4853 # Alanine, aspartate and glutamate metabolism Arginine biosynthesis Cysteine and methionine metabolism Arginine and proline metabolism Tyrosine metabolism Phenylalanine metabolism Phenylalanine, tyrosine and tryptophan biosynthesis 1.61 0.0016 *** 48 S-adenosylmethionine...”
- “...I/II/III large subunit [EC:2.2.1.6] alr2811 valine- pyruvate aminotransferase [EC:2.6.1.66] alr3809 carbamoyl-phosphate synthase large subunit [EC:6.3.5.5] alr4853 aspartate aminotransferase [EC:2.6.1.1] alr3293 threonine synthase [EC:4.2.3.1] Transporters alr0140 peptide/nickel transport system substrate- binding protein periplasmic oligopeptide- binding ABC transporter Quorum sensing alr1554 ATP-binding cassette, subfamily B alr4164 periplasmic amino...”
- Identification of two genes required for heptadecane production in a N2-fixing cyanobacterium Anabaena sp. strain PCC 7120
Gibbons, AMB Express 2018 - “...nitrogen starvation (Flaherty et al. 2011 ). The glnA promoter was successfully used for complementing alr4853 mutant in Anabaena 7120 (Xu et al. 2015 ). DR935(pZR2248) contained both genes in a two gene operon ( ado aar ) under the glnA promoter, yet did not recover...”
- The Anabaena sp. PCC 7120 Exoproteome: Taking a Peek outside the Box
Oliveira, Life (Basel, Switzerland) 2015 - “...metabolism - Alr4794 Alr4794 protein NO 3 , NH 4 + Conserved hypothetical protein Anabaena Alr4853 Aspartate aminotransferase N 2 Amino acid biosynthesis - Alr4907 Ornithine carbamoyltransferase N 2 , NO 3 , NH 4 + Amino acid biosynthesis - Alr4976 Phosphodiesterase/alkaline phosphatase D NO 3...”
- “...as processes regarding amino acid biosynthesis and processing (All2533, Alr0237, Alr0880, Alr0996, Alr1381, All4287, Alr1742, Alr4853, All2315, Alr1004, Alr1313, Alr2771, Alr0608, Alr1080, Alr2328 Alr4907, All1683, All4464), sugar breakdown and processing (Alr3608, Alr4448, All3964, All0167, All0168, All0875, Alr0169, Alr2190, All4539) and phosphor scavenge and transport (All0207, All2843,...”
- Catabolic function of compartmentalized alanine dehydrogenase in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120
Pernil, Journal of bacteriology 2010 - “...which could result from a transamination reaction. ORF alr4853 of the Anabaena genome encodes an aspartate transaminase (14) that is also the predicted Anabaena...”
P23034 Aspartate aminotransferase from Bacillus sp. (strain YM-2)
34% identity, 90% coverage
- Profiling the orphan enzymes.
Sorokina, Biology direct 2014 - “...shares more than 50% of amino acid identity with biochemically characterized aspartate aminotransferases (UniProt ACs P23034, Q59228). This activity is more coherent with the asparaginyl tRNA synthetase genomic context than the asparagine aminotransferase activity proposed by Yamada et al., an activity described only in eukaryotes for...”
- Identification of the biosynthetic gene cluster for 3-methylarginine, a toxin produced by Pseudomonas syringae pv. syringae 22d/93
Braun, Applied and environmental microbiology 2010 - “...Bsp Asp, Bacillus sp. aspartate aminotransferase (P23034); Sso Asp, Sulfolobus solfataricus aspartate aminotransferase (X16505); Cgl 2877, Corynebacterium...”
- Novel archaeal alanine:glyoxylate aminotransferase from Thermococcus litoralis
Sakuraba, Journal of bacteriology 2004 - “...PSerATye PSerATr PheATpd DapaATec DapaATbs P00509 P14909 P23034 P23542 P00504 P00508 P26563 P25409 NP_418478a P04694 P06986 P07172 P16246 P17736 P17731 P18335...”
- Imidazole acetol phosphate aminotransferase in Zymomonas mobilis: molecular genetic, biochemical, and evolutionary analyses
Gu, Journal of bacteriology 1995 - “...Bsp Asp, Bacillus species aspartate aminotransferase (P23034); Sso Asp, Sulfolobus solfataricus aspartate aminotransferase (X16505); Rat Tyr, rat tyrosine...”
aspB / GB|CAB14153.1 aspartate transaminase; EC 2.6.1.1 from Bacillus subtilis (see paper)
aspB / AAB38454.1 aspartate aminotransferase from Bacillus subtilis (see paper)
P53001 Aspartate aminotransferase from Bacillus subtilis (strain 168)
BSU22370 aspartate aminotransferase from Bacillus subtilis subsp. subtilis str. 168
33% identity, 90% coverage
- Characterization of a bordetella pertussis diaminopimelate (DAP) biosynthesis locus identifies dapC, a novel gene coding for an N-succinyl-L,L-DAP aminotransferase
Fuchs, Journal of bacteriology 2000 - “...(36) (HP0624), B. subtilis (GenBank accession number P53001), and Synechocystis sp. (GenBank accession number D64000). Amino acids identical or similar in...”
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis
Richts, Frontiers in molecular biosciences 2019 - “...Czaplewski et al., 1992 ; Brinsmade et al., 2014 ; Ogura and Kanesaki, 2018 AspB BSU22370 No 2.6.1.1 1J32 ( Phosmidium lapideum , 45%) Aspartate aminotransferase Aspartate biosynthesis Unknown AlaT (41%), PatA (41%)/PLP AAT class I family Zhao et al., 2018 CysK BSU00730 No 2.5.1.47 1Y7L...”
ATU_RS10725 pyridoxal phosphate-dependent aminotransferase from Agrobacterium fabrum str. C58
33% identity, 89% coverage
OP10G_2150 pyridoxal phosphate-dependent aminotransferase from Fimbriimonas ginsengisoli Gsoil 348
35% identity, 92% coverage
GOX0190 Aspartate aminotransferase A from Gluconobacter oxydans 621H
35% identity, 89% coverage
CC1534 aspartate aminotransferase from Caulobacter crescentus CB15
CCNA_01603 aspartate aminotransferase from Caulobacter crescentus NA1000
34% identity, 95% coverage
- Transcriptional profiling of Caulobacter crescentus during growth on complex and minimal media
Hottes, Journal of bacteriology 2004 - “...aldAd CC0945, oxidoreductase, glucose-methanolcholine family CC1534, aspartate aminotransferase, aspc CC2152, secreted protein, putativec CC2153, polysaccharide...”
- The complex logic of stringent response regulation in Caulobacter crescentus: starvation signalling in an oligotrophic environment
Boutte, Molecular microbiology 2011 - “...NA1000 cysE hMu CCNA_02734 ( Huitema et al , 2006 ) 1167 NA1000 asp mar414 CCNA_01603 ( Huitema et al , 2006 ) 329 NA1000 thrB mar414 CCNA_03475 ( Huitema et al , 2006 ) 316 NA1000 proC hMu CCNA_00528 ( Huitema et al , 2006...”
BMEI0516 ASPARTATE AMINOTRANSFERASE A from Brucella melitensis 16M
33% identity, 85% coverage
- Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics
He, Frontiers in cellular and infection microbiology 2012 - “...E: AMINO ACID TRANSPORT AND METABOLISM 8 aroC BMEI1506 Macrophages, HeLa, mice 11119550 9 aspC BMEI0516 HeLa 12761078 10 BMEII0626 BMEII0626 Mice, macrophages, HeLa 14979322 11 BMEII0923 BMEII0923 Mice 14979322 12 cysK BMEI0933 Mice, macrophages, HeLa 14979322 13 dppA BMEI0433 Mice, macrophages, HeLa 14979322 14 glnA...”
- Global analysis of quorum sensing targets in the intracellular pathogen Brucella melitensis 16 M
Uzureau, Journal of proteome research 2010 - “...no. F.C. sequence C % score m/z charge method babR , pH 47 AA metabolism BMEI0516 Aspartate aminotransferase AAL51697.1 0.68 QAAIAAINR 2 51 464,2757 2+ Q-TOF Central metabolism BMEI0791 Isocitrate deshydrogenase AAL51972.1 0.68 ASFNYGLKR 2 49 528,2996 2+ Q-TOF Central metabolism BMEI1436 pyruvate phosphate dikinase AAL52617.1...”
Rru_A1135 Aminotransferase, class I and II from Rhodospirillum rubrum ATCC 11170
35% identity, 88% coverage
- The metabolic pathways of carbon assimilation and polyhydroxyalkanoate production by Rhodospirillum rubrum in response to different atmospheric fermentation
Tang, PloS one 2024 - “...Pyruvate- flavodoxin oxidoreductase ND ND 0.002392 0.561 Threonine dependent branched-chain amino acid (BCAA) biosynthesis pathways Rru_A1135 Aminotransferase ND ND 0.003836 0.325 Rru_A0743 Aspartate kinase ND ND 0.013116 0.284 Rru_A1196 Aspartate semialdehyde dehydrogenase ND ND 2.20E-34 2.005 Rru_A3053 Homoserine kinase 1.21E-06 0.938103 ND ND Rru_A0467 Acetolactate synthase...”
- “...enzymes in reductive threonine-dependent pathway were found to be upregulated in the syngas condition ( Rru_A1135 , p = 0.003, fold change = 0.325; Rru_A0743 , p = 0.013, fold change = 0.284; Rru_A1196 , p = 2.20E-34, fold change = 2.005). One oxaloacetate and one...”
AAT_RHIML / Q06191 Aspartate aminotransferase; AAT; AspAT; Transaminase A; EC 2.6.1.1 from Rhizobium meliloti (Ensifer meliloti) (Sinorhizobium meliloti) (see paper)
33% identity, 89% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate.
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
disruption phenotype: Insertion mutant is prototroph and is able to carry out symbiotic nitrogen fixation.
Clocel_2059 pyridoxal phosphate-dependent aminotransferase from Clostridium cellulovorans 743B
33% identity, 91% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...Table 1 and Supplementary Table 2 ). In addition, up-regulated proteins include four aminotransferases (Clocel_1948, Clocel_2059, Clocel_2390, Clocel_3812). The role of amino acids in the cellular stress response is a well-known concern. Increase in pyrroline-5-carboxylate reductase and intracellular levels of proline in response to butanol stress...”
BruAb1_1488 pyridoxal phosphate-dependent aminotransferase from Brucella abortus bv. 1 str. 9-941
Q57C18 Aminotransferase from Brucella abortus biovar 1 (strain 9-941)
BAB1_1514 1-aminocyclopropane-1-carboxylate synthase:Aminotransferase, class-I:Aminotransferase, class I and II from Brucella melitensis biovar Abortus 2308
33% identity, 90% coverage
- Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle
Lee, Veterinary research 2015 - “...space E: Amino acid transport and metabolism 151 aspC 3339882 aspartate aminotransferase YP_222177.1 Q57C18 400 BruAb1_1488 193 43812 43554 5.94 18 unknown F: Nucleotide transport and metabolism 161 BruAb1_0775 3339474 3-hydroxyisobutyryl-CoA hydrolase YP_221504.1 Q57DZ1 349 BruAb1_0775 258 38120 37802 4.84 27 unknown H: Coenzyme transport and...”
- Immunoproteomic identification of immunodominant antigens independent of the time of infection in Brucella abortus 2308-challenged cattle
Lee, Veterinary research 2015 - “...25 periplasmic space E: Amino acid transport and metabolism 151 aspC 3339882 aspartate aminotransferase YP_222177.1 Q57C18 400 BruAb1_1488 193 43812 43554 5.94 18 unknown F: Nucleotide transport and metabolism 161 BruAb1_0775 3339474 3-hydroxyisobutyryl-CoA hydrolase YP_221504.1 Q57DZ1 349 BruAb1_0775 258 38120 37802 4.84 27 unknown H: Coenzyme...”
- Proteomics of Brucella
Poetsch, Proteomes 2020 - “...regulator (BAB2_0377); DnaK: chaperone protein (BAB1_2129); SurA: trigger factor-peptidyl prolyl cis-trans isomerase (BAB1_0917); AspC: aminotransferase (BAB1_1514); CHG: choloylglycine hydrolase (BAB1_1488); SerC: phosphoserine transaminase (BAB1_1699); GroES: 10 kDa chaperonin (BAB2_0190); IalB: invasion protein B (BAB1_0368); FtsZ: cell division protein FtsZ (BAB1_144); MinD: septum site-determining protein (BAB2_0883); TolB:...”
- An account of evolutionary specialization: the AbcR small RNAs in the Rhizobiales
Sheehan, Molecular microbiology 2018 - “...bab1_1515 and bab1_1516 , both of which encode small hypothetical proteins. abcR2 is flanked by bab1_1514 , encoding an aspartate aminotransferase, and bab1_1517 , encoding the LysR-type transcriptional regulator VtlR ( Figure 1C ). VtlR, for v irulence-associated t ranscriptional L ysR-family r egulator, is the...”
- Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genome
Lamontagne, BMC genomics 2010 - “...YbgT BAB1_0810 RpsI; S9 BAB1_1384 Cibk BAB1_1858 RplU; L21 BAB2_0869 HlyD BAB1_0830 NDH-1 subunit I BAB1_1514 AspC BAB1_1984 LysA BAB2_1002 NqoB Locus tags and descriptions of proteins are indicated and proteins are organized by predicted subcellular localization. Correction of five pseudogene annotations In previous studies using...”
AF_1623 pyridoxal phosphate-dependent aminotransferase from Archaeoglobus fulgidus DSM 4304
36% identity, 89% coverage
- A novel methoxydotrophic metabolism discovered in the hyperthermophilic archaeon Archaeoglobus fulgidus
Welte, Environmental microbiology 2021 - “...& AF_2185/6), 2oxoglutarate/2oxoacid ferredoxin oxidoreductase KorABDG (AF_046871), glutamate synthase GltB13 (AF_09524), aspartate aminotransferase AspBC (AF_0409, AF_1623, AF_2129, AF_2366, AF_1417), isocitrate dehydrogenase Icd (AF_0647), aconitase Acn (AF_1963), citrate synthase CitZ (AF_1340), sulfate adenylyltransferase Sat (AF_1667), adenylylsulfate reductase AprAB (AF_166970), dissimilatory sulfite reductase DsrAB (AF_04234), DsrC (AF_2228), quinonemodifying...”
FQU82_02901 pyridoxal phosphate-dependent aminotransferase from Acinetobacter baumannii
36% identity, 88% coverage
- Transcriptomic analysis reveals the regulatory role of quorum sensing in the Acinetobacter baumannii ATCC 19606 via RNA-seq
Xiong, BMC microbiology 2022 - “...FQU82_02187, FQU82_01589( paaK ), FQU82_01581( paaB ), FQU82_01580( paaA ), and FQU82_01583( paaD ), while FQU82_02901 was found to be downregulated. Among the DEGs involved in the arginine biosynthesis, FQU82_00067 and FQU82_01752 were upregulated, whereas FQU82_01177, FQU82_02901, FQU82_01050 ( ureC ), FQU82_01049 ( ureB ), and...”
Cthe_0755 aspartate aminotransferase from Clostridium thermocellum ATCC 27405
32% identity, 89% coverage
101251438 / A0A3Q7IS73 2-oxo-4-methylthiobutanoate-glutamine aminotransferase monomer (EC 2.6.1.117) from Solanum lycopersicum (see paper)
34% identity, 77% coverage
bll7416 aspartate aminotransferase A from Bradyrhizobium japonicum USDA 110
34% identity, 88% coverage
Q6F9C7 Aminotransferase from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)
35% identity, 88% coverage
A0A6F8T0V6 aspartate transaminase (EC 2.6.1.1) from Geobacillus sp. MAS1 (see paper)
33% identity, 90% coverage
AF_2129 pyridoxal phosphate-dependent aminotransferase from Archaeoglobus fulgidus DSM 4304
32% identity, 90% coverage
- A novel methoxydotrophic metabolism discovered in the hyperthermophilic archaeon Archaeoglobus fulgidus
Welte, Environmental microbiology 2021 - “...AF_2185/6), 2oxoglutarate/2oxoacid ferredoxin oxidoreductase KorABDG (AF_046871), glutamate synthase GltB13 (AF_09524), aspartate aminotransferase AspBC (AF_0409, AF_1623, AF_2129, AF_2366, AF_1417), isocitrate dehydrogenase Icd (AF_0647), aconitase Acn (AF_1963), citrate synthase CitZ (AF_1340), sulfate adenylyltransferase Sat (AF_1667), adenylylsulfate reductase AprAB (AF_166970), dissimilatory sulfite reductase DsrAB (AF_04234), DsrC (AF_2228), quinonemodifying oxidoreductase...”
patA / P16524 N-acetyl-L,L-diaminopimelate aminotransferase from Bacillus subtilis (strain 168) (see 3 papers)
DAPX_BACSU / P16524 Probable N-acetyl-LL-diaminopimelate aminotransferase; Putative aminotransferase A; EC 2.6.1.- from Bacillus subtilis (strain 168) (see paper)
BSU14000 aminotransferase A from Bacillus subtilis subsp. subtilis str. 168
33% identity, 91% coverage
- function: Essential for murein biosynthesis (PubMed:29280348). Probably catalyzes the conversion of L-2-acetamido-6-oxopimelate to N-acetyl-LL- 2,6-diaminopimelate (Probable).
catalytic activity: N-acetyl-(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = L-2-acetamido-6-oxoheptanedioate + L-glutamate (RHEA:25315)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis
Richts, Frontiers in molecular biosciences 2019 - “...AbrB (), CodY(), ScoC () PLP AAT class IV family Nicolas et al., 2012 PatA BSU14000 Yes 2.6.1.- 1GDE ( Pyrococcus sp., 42%) N -Acetyl-L,L-diaminopimelate aminotransferase Biosynthesis of lysine and peptidoglycan Unknown AlaT (41%), AspB (41%)/PLP AAT class I family Nicolas et al., 2012 ; Rueff...”
- The Blueprint of a Minimal Cell: MiniBacillus
Reuß, Microbiology and molecular biology reviews : MMBR 2016 - “...S. pneumoniae B. anthracis Corynebacterium glutamicum B. anthracis BSU14000 BSU14190 BSU32170 Yes Yes Yes 3.5.1.47 5.1.1.7 1GDE 1YSJ 2OTN P. horikoshii B....”
AT1G77670 aminotransferase class I and II family protein from Arabidopsis thaliana
35% identity, 79% coverage
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond
Apodiakou, Journal of experimental botany 2023 - “...BGLU28 AT2G44460 3 Carbon metabolism, glucosinolate hydrolysis Gaudet et al. (2011) Pyridoxal phosphate (PLP) protein AT1G77670 3 Carbon metabolism, transaminase activity Depuydt and Vandepoele (2021) PYD4 AT3G08860 3 Response to nitrogen, transaminase activity Zrenner et al. (2009) SIP1;2 AT5G18290 3 Transporter activity Ishikawa et al. (2005)...”
- “...family AT5G40670 No Yes Yes FAD binding barberine AT4G20820 Yes No Yes Pyridoxal phosphate (PLP) AT1G77670 Yes No Yes NmrA-like negative transcriptional regulator AT1G75280 No Yes Yes hypothetical protein AT2G32487 Yes No Yes Information about the regulation of the extended OAS cluster co-expression gene network from...”
- The genome wide analysis of Tryptophan Aminotransferase Related gene family, and their relationship with related agronomic traits in Brassica napus
Cheng, Frontiers in plant science 2022 - “..., AT2G24850 , AT2G24850 , AT4G08040 , AT5G49810 , AT5G51690 , AT1G23320 , AT1G62960 , AT1G77670 , AT2G13810 , AT3G61510 , AT4G11280 , AT4G24670 , AT4G28410 , AT5G36160 ) had less than four copies in B. napus ( Table S3 ), by simply calculating the Arabidopsis...”
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms in Arabidopsis Thaliana
Willems, Frontiers in plant science 2021 - “...triad family protein 3 (HIT3) Met19 Ac-A AT5G63890 Histidinol dehydrogenase (HDH) Met18 NH 2 /Ac-MK AT1G77670 Pyridoxal phosphate-dependent transferase Met41 Ac-T AT5G13050 5-Formyltetrahydrofolate cycloligase (5-FCL) Met43 NH 2 /Ac-S AT5G12040 -amidase Met63 Ac-A AT4G08790 Deaminated glutathione amidase Met29 Ac-A AT5G03370 Acylphosphatase family Met66 NH 2 /Ac-T...”
- The genetic architecture of constitutive and induced trichome density in two new recombinant inbred line populations of Arabidopsis thaliana: phenotypic plasticity, epistasis, and bidirectional leaf damage response
Bloomer, BMC plant biology 2014 - “...x Sf-2 Constitutive; pLOD=7.32 SSC1 1 107 102-109.7 7.84 Sf 7.7 ; St 6.7 GL2; At1g77670 1 ; JAZ2 SSC2 2 61 52-68 7.15 Sf 6.6; St 7.4 URM9; TTG2 1 SSC3 3 72 9-76 6.77 Sf 7.4 ; St 6.8 SSC4 4 29 25-32 9.88...”
- “...1 102 87.92-109 12.34 Sf 9.3 ; St 8.3 RGL1; JAZ9 1 ; JAZ2; GL2; At1g77670 SSD2 3 7.8 3-13 6.89 Sf 8.9 ; St 8.0 - SSD3 5 4 0-108 6.99 Sf 8.2; St 8.8 - TOTAL 26.22 St-0 x Sf-2 Response; pLOD=2.51 SSR1 1...”
- Mining the soluble chloroplast proteome by affinity chromatography
Bayer, Proteomics 2011 - “...dehydrogenase O + AT1G76690 OPR2; 12-oxophytodienoate reductase O + AT1G77122 Unknown protein C + + AT1G77670 a) Aminotransferase class I and II family protein M + AT1G77930 DNAJ heat shock N-terminal domain-containing protein C + WP AT1G79530 c) GAPCP-1; glyceraldehyde-3-phosphate dehydrogenase C + WP, LFA AT1G79870...”
- “...C C C C + AT1G22410 DAS 2-Dehydro-3-deoxyphosphoheptonate aldolase C C C C + + AT1G77670 ATF Aminotransferase class I and II family protein M C O O + AT2G17240 CUP2 Chloroplast unknown protein 1 C C C C + AT2G25870 HAC Haloacid dehalogenase-like family protein...”
- A systems approach reveals regulatory circuitry for Arabidopsis trichome initiation by the GL3 and GL1 selectors
Morohashi, PLoS genetics 2009 - “...LEA protein + + + + At4g20960 Cytidine/deoxycytidylate deaminase family protein + + + + At1g77670 Aminotransferase + + + + At3g10113 MYB transcription factor + + GL3/GL1 Direct Target Identified by ChIP-chip At5g04470 SIM + + + At3g12280 RBR1 + + + At2g26250 FDH +...”
- “...identified as putatively bound by both GL3 and GL1. Six of the seven genes ( At1g77670 , At3g50790 / At3g50800 , At4g20960 , At5g28350 and At5g52510 ) were confirmed as recognized by both GL3 and GL1, and one ( At3g10113 ) showed no binding by either...”
AAT_MUSP7 / C6C2Z3 Aspartate aminotransferase; AAT; AspAT; EC 2.6.1.1 from Musicola paradisiaca (strain Ech703) (Dickeya paradisiaca) (Dickeya dadantii) (see paper)
33% identity, 90% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:25070637). Has very weak prephenate aminotransferase activity (PubMed:25070637).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
FAD_1098 pyridoxal phosphate-dependent aminotransferase from Ferroplasma acidiphilum
31% identity, 90% coverage
- Metabolic and evolutionary patterns in the extremely acidophilic archaeon Ferroplasma acidiphilum YT
Golyshina, Scientific reports 2017 - “...12, fumarate hydratase (FAD_1630); 13, malate dehydrogenase (FAD_0718); 14, glutamate dehydrogenase (FAD_0434); 15, aspartate aminotransferase (FAD_1098); 16, phosphoenolpyruvate synthase (FAD_1233); 17, phosphoglycerate mutase (FAD_0440, FAD_1169, FAD_1350); 18,2-phosphoglycerate kinase (FAD_1810); 19, glyceraldehyde-3-phosphate dehydrogenase (FAD_0549); 20, triosephosphate isomerase (FAD_0107); 21, fructose-2,6-bisphosphatase (FAD_0332); 22,6-phosphofructokinase (FAD_0353); 23, bifunctional phosphoglucose/phosphomannose isomerase...”
- “...of nitrogen metabolism. Additionally, glutamate can be also formed from 2-OG by an aspartate aminotransferase (FAD_1098) yielding oxaloacetate (Fig. 4 ). Glycolysis/Gluconeogenesis Growth on amino acids requires a gluconeogenic pathway for carbohydrate synthesis 48 and in line with that all genes for a reverse glycolytic pathway...”
GBO44_RS03485, WP_159211138 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Pediococcus acidilactici
33% identity, 89% coverage
- The aminotransferase Aat initiates 3-phenyllactic acid biosynthesis in Pediococcus acidilactici
Wenger, Frontiers in microbiology 2023 - “...which does not comply with these terms. The function of the aminotransferase Aat (GenBank Protein WP_159211138) from Pediococcus acidilactici FAM 18098 was studied in vivo . For this purpose, the gene was replaced with an erythromycin resistance gene using the temperature-sensitive Escherichia coli-Pediococcus shuttle plasmid pSET4T_...”
- “...FAM 18098 for genes encoding aminotransferases, an aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme (GenBank Protein WP_159211138) was identified that may be involved in the formation of -aminobutyric acid and alanine ( Wenger et al., 2020 ). In order to analyze the enzymatic activity of this aminotransferase,...”
- Identification of a species-specific aminotransferase in Pediococcus acidilactici capable of forming α-aminobutyrate
Wenger, AMB Express 2020 - “...and aat_Rv (5-TGCTCGTTTAGCTTTGAGG-3) were designed to amplify the complete aminotransferase-coding gene presented by the locus GBO44_RS03485. The primer aat_Rv did not contain the stop codon for cloning reasons. The amplification reactions were performed using the AmpliTaq Gold DNA Polymerase (Thermo Fisher Scientific) and applying the manufacturers...”
- “...for genes encoding aminotransferases to follow this hypothesis. Six genes represented by GBO44_RS00380, GBO44_RS02360, GBO44_RS03135, GBO44_RS03485, GBO44_RS04865, and GBO44_RS09300 were annotated to encode amino acid aminotransferases (Fig. 2 ). BLAST searches were performed to obtain more clarity about the function of these aminotransferases. First, it was...”
MPNT_250010 pyridoxal phosphate-dependent aminotransferase from Candidatus Methylacidithermus pantelleriae
34% identity, 93% coverage
1o4sB / Q9X0Y2 Crystal structure of aspartate aminotransferase (tm1255) from thermotoga maritima at 1.90 a resolution (see paper)
33% identity, 94% coverage
- Ligand: pyridoxal-5'-phosphate (1o4sB)
AAT_THEMA / Q9X0Y2 Aspartate aminotransferase; AspAT; Transaminase A; EC 2.6.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
TM1255 aspartate aminotransferase from Thermotoga maritima MSB8
33% identity, 94% coverage
- catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Crystal structure of an aspartate aminotransferase (TM1255) from Thermotoga maritima at 1.90 A resolution
Schwarzenbacher, Proteins 2004 (PubMed)- “...of an Aspartate Aminotransferase (TM1255) From Thermotoga maritima at 1.90 A Resolution Robert Schwarzenbacher,1,4 Lukasz Jaroszewski,1,4 Frank von Delft,1,6...”
- “...6 Scripps Research Institute, La Jolla, California The TM1255 gene of Thermotoga maritima encodes an aspartate aminotransferase (AspC-1, EC 2.6.1.1), with a...”
LOC105058225 uncharacterized protein LOC105058225 from Elaeis guineensis
34% identity, 75% coverage
jhp0615 ASPARTATE AMINOTRANSFERASE from Helicobacter pylori J99
32% identity, 97% coverage
- Growth phase-dependent response of Helicobacter pylori to iron starvation
Merrell, Infection and immunity 2003 - “...JHP0098 JHP0099 JHP0570 JHP0594 JHP0615 3-Deoxy-D-arabino-heptulosonate-7-phosphate synthase, aroF Cystathionine gamma-synthase, metB Cysteine synthetase,...”
- “...Amino acid biosynthesis *HP0649 *#HP0672 JHP0594 JHP0615 Aspartate ammonia-lyase, aspA Solute binding signature and mitochondrial signature protein, aspB...”
aspC / GB|CAA63799.1 aspartate transaminase; EC 2.6.1.1 from Geobacillus stearothermophilus (see paper)
Q59228 Aspartate aminotransferase from Geobacillus stearothermophilus
34% identity, 90% coverage
- Data on a thermostable enzymatic one-pot reaction for the production of a high-value compound from l-arabinose
Bawn, Data in brief 2018 - “...violaceum (%) Deinococcus radiodurans DSM 20539 Q9RWP3 pQR1746 78 38 31 Geobacillus stearothermophilus DSM 22 Q59228 pQR1756 (TAm Gste ) 25 38 40 Thermobifida fusca strain YX Q47LH8 pQR1748 26 37 41 Thermotoga maritima DSM 3109 G4FE93 pQR1749 30 58 31 Deinococcus geothermalis DSM 11300 Q1IZC2...”
- Profiling the orphan enzymes.
Sorokina, Biology direct 2014 - “...more than 50% of amino acid identity with biochemically characterized aspartate aminotransferases (UniProt ACs P23034, Q59228). This activity is more coherent with the asparaginyl tRNA synthetase genomic context than the asparagine aminotransferase activity proposed by Yamada et al., an activity described only in eukaryotes for asparagine...”
Q98I67 Aminotransferase from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
34% identity, 90% coverage
- Cyclic Isothiocyanate Goitrin Impairs Lotus japonicus Nodulation, Affects the Proteomes of Nodules and Free Mesorhizobium loti, and Induces the Formation of Caffeic Acid Derivatives in Bacterial Cultures
Jeong, Plants (Basel, Switzerland) 2024 - “...Q9836B, a protein for the interconversion of serine and glycine was downregulated, as was aminotransferase Q98I67. Also, nitrogen regulatory protein Q98N18 was reduced. Q98M73, with a function in glycerophospholipid biosynthesis, Q98BH8, an enzyme for the introduction of cis unsaturation into fatty acids, and Q984T2, with a...”
O25383 Aminotransferase from Helicobacter pylori (strain ATCC 700392 / 26695)
HP0672 solute-binding signature and mitochondrial signature protein (aspB) from Helicobacter pylori 26695
33% identity, 97% coverage
- Outer Membrane Vesicles Secreted by Helicobacter pylori Transmitting Gastric Pathogenic Virulence Factors
Wei, ACS omega 2022 - “...P56145 pheT 108 P56031 rplC 109 O24914 HP_0087 110 O25732 Cad 111 O25116 pyrG 112 O25383 HP_0672 113 O25787 HP_1173 114 O25166 HP_0410 115 O25052 AddB 116 O25465 HP_0773 117 O25008 iscS 118 O25067 amiE 119 P56060 kdsA 120 O25658 HdhA 121 O25936 fbp 122 P55982...”
- “...O25368 mqnE 246 P55970 grpE 247 O25218 Omp11 248 P64653 HP_0122 249 O25234 HP_0492 250 O25383 HP_0672 251 O25762 HP_1143 252 P66637 HP_1143 253 O26039 plsY 254 O24864 CheV 255 O26052 HP_1524 256 O25073 DppF 257 O24999 mrp 258 O25684 HP_1043 259 P56041 rplP 260 O25288...”
- Structural and functional aspects of the Helicobacter pylori secretome
Zanotti, World journal of gastroenterology 2014 - “...z b' b' 3EZS 3FNM, 2QM6, 2QMC, 2NQO 1J2Z O25383 O25743 P56463 O25798 O25927 P42383 P0A0R3 O24973 O25076 P56098 O25646 O25751 2KI2 2XRH O25501 O25506 P56029...”
- Structural and functional aspects of the Helicobacter pylori secretome
Zanotti, World journal of gastroenterology 2014 - “...Zanotti G et al . Structural and functional aspects HP0672 HP1118 HP1178 HP1186 HP1375 Others HP0010 HP0011 HP0166 HP0305 HP0743 HP1000 HP1126 Member of the...”
- “...in the secretion of other enzymes, including HP0672, a member of the PLP-dependent aminotransferase superfamily clan, is not evident. The existence...”
- Targeted identification of glycosylated proteins in the gastric pathogen Helicobacter pylori (Hp)
Champasa, Molecular & cellular proteomics : MCP 2013 - “...inner membrane 8 2 10.6 HP0330 O25097 ilvC 38.5 ketol-acid reductoisomerase cytoplasm 2 2 10.3 HP0672 AAD07733 aspB 45.1 mitochondrial signature protein cytoplasm 2 2 10.0 HP1103 O25731 glk 38.6 glucokinase cytoplasm 11 2 9.9 HP0221 AAD07289 38.3 nifU-like cytoplasm 2 2 9.9 HP0604 AAD07669 hemE...”
- Iron and pH homeostasis intersect at the level of Fur regulation in the gastric pathogen Helicobacter pylori
Gancz, Infection and immunity 2006 - “...of California, Berkeley 4.2 2.8 2.3 0.4 HP0672 JHP0626 JHP0742 JHP0743 JHP0810 JHP0811 JHP0819 JHP0939 JHP1258 Biopolymer transport protein, exbD...”
- Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants
Thiele, Journal of bacteriology 2005 - “...(CHORM), HP0672 (EHGLAT, PHETA1, TYRTA),...”
- Gene expression profiling of Helicobacter pylori reveals a growth-phase-dependent switch in virulence gene expression
Thompson, Infection and immunity 2003 - “...1.9 1.6 hydB Amino acid biosynthesis HP0649e HP0672 Biosynthesis of cofactors HP1582d,e HP1583d Cell envelope HP1373 HP1429 Cellular processes HP0630 Central...”
- The society of genes: networks of functional links between genes from comparative genomics
Yanai, Genome biology 2002 - “...the exception of a lack of a fusion link between HP1279 and HP1282. HP0154 and HP0672 are present in the network but are not linked to other genes known to act in this pathway. Overall, in E. coli, 26 clusters of seven or more genes corresponding...”
MTH_1894 guanitoxin biosynthesis PLP-dependent (S)-gamma-hydroxy-L-arginine cyclodehydratase GntC from Methanothermobacter thermautotrophicus str. Delta H
34% identity, 89% coverage
M1E0E5 Uncharacterized protein from Solanum tuberosum
34% identity, 57% coverage
LOC104882468 uncharacterized protein LOC104882468 from Vitis vinifera
36% identity, 71% coverage
Dde_3596 Aspartate aminotransferase (aspB) from Desulfovibrio desulfuricans G20
34% identity, 97% coverage
OsIDI4 / FAA00314.1 aminotransferase from Oryza sativa Japonica Group (see paper)
36% identity, 74% coverage
APA386B_942 pyridoxal phosphate-dependent aminotransferase from Acetobacter pasteurianus 386B
35% identity, 90% coverage
- Genome-Scale Metabolic Reconstruction of Acetobacter pasteurianus 386B, a Candidate Functional Starter Culture for Cocoa Bean Fermentation
Pelicaen, Frontiers in microbiology 2019 - “...aminotransferase (EC 2.6.1.1) was linked to three genes, likely encoding this enzyme (APA386B_861, APA386B_862, and APA386B_942). These were not predicted to be homologous to the E. coli aspartate aminotransferase gene (b0928, aspC ) but shared considerable sequence identity with curated SwissProt sequences from other bacteria with...”
- “...reaction was tentatively associated to the aspartate aminotransferases encoded in the genome (APA386B_861, APA386B_862, and APA386B_942). Since A. pasteurianus 386B was able to grow in a defined medium containing sulfate as the sole sulfur source, sulfate assimilation was assumed ( Figure 3B ). Indeed, import of...”
DVU3223 aspartate aminotransferase from Desulfovibrio vulgaris Hildenborough
35% identity, 97% coverage
- Absence of biofilm adhesin proteins changes surface attachment and cell strategy for <i>Desulfovibrio vulgaris</i> Hildenborough
Pickens, Journal of bacteriology 2025 - “...DVU2512 S-adenosyl-methyltransferase MraW M Cytoplasm 2.013 0.047 DVU2070 TPR domain protein S Unknown 2.102 0.023 DVU3223 Aspartate aminotransferase E Cytoplasm 2.107 0.006 DVU0091 Conserved hypothetical protein Unknown 2.211 0.032 DVU0606 Transcriptional regulator, ArsR family/methyltransferase, UbiE/COQ5 family H Cytoplasm 2.256 0.048 DVU1346 Exodeoxyribonuclease VII, large subunit L...”
- Genetic Basis of Chromate Adaptation and the Role of the Pre-existing Genetic Divergence during an Experimental Evolution Study with Desulfovibrio vulgaris Populations
Shi, mSystems 2021 - “...genes were mutated in both EC-Cr and ES-Cr populations, including DVU0426, DVU1092, DVU2206, DVU3134, and DVU3223. DVU1571 was mutated in both the AN-Cr and EC-Cr populations, DVU2394 was mutated in both the AN-Cr and ES-Cr populations, and DVU1834 and DVU2894 were mutated in all three groups....”
- “...of DVU1344 increased under heat shock ( 52 ); the expression of DVU2206, DVU2894, DVU3134, DVU3223, and DVU3230 was associated with NaCl stress ( 36 , 53 , 54 ); and expression of DVU1834 decreased under high pH ( 55 ). Moreover, expression of 10 of...”
- Characterization of NaCl tolerance in Desulfovibrio vulgaris Hildenborough through experimental evolution
Zhou, The ISME journal 2013 - “...5-dehydrogenase (aroE) DVU2492 N-(5-phosphoribosyl)anthranilate isomerase (trpF-2) DVU3223 Aspartate aminotransferase (aspB) LS4D LS4D-100 LS4D-250 ES vs An ES...”
Dshi_0762 aminotransferase class I and II from Dinoroseobacter shibae DFL 12
34% identity, 89% coverage
- Dinoroseobacter shibae Outer Membrane Vesicles Are Enriched for the Chromosome Dimer Resolution Site dif
Wang, mSystems 2021 - “...protein Transporter 9.51E03 1.98E03 0.95 Dshi_0318 Glutamate/glutamine/aspartate/asparagine ABC transporter Periplasmic protein Transporter 9.23E03 1.33E03 0.92 Dshi_0762 Aminotransferase class I and II Intracellular protein Amino acid metabolism 7.45E03 1.21E03 0.75 Dshi_0273 Translation elongation factor G Intracellular protein Translation 7.22E03 1.46E03 0.72 Dshi_3318 d -3-Phosphoglycerate dehydrogenase Intracellular protein...”
OG1RF_11417 pyridoxal phosphate-dependent aminotransferase from Enterococcus faecalis OG1RF
34% identity, 90% coverage
- Enterococcal quorum-controlled protease alters phage infection
Sheriff, FEMS microbes 2024 - “...Preprotein translocase subunit SecY 86.97 No, not present OG1RF_11743 TetR/AcrR family transcriptional regulator 52.26 Yes OG1RF_11417 Pyridoxal phosphate-dependent aminotransferase 47.66 Yes OG1RF_10804 ATP-binding protein 45.55 Yes OG1RF_12485 Alkaline phosphatase family protein 40.55 Yes phoU ( OG1RF_11465 ) Phosphate signaling complex protein 40.16 Yes OG1RF_10823 AAA family...”
RD1_3892 aspartate aminotransferase from Roseobacter denitrificans OCh 114
34% identity, 90% coverage
- Carbohydrate metabolism and carbon fixation in Roseobacter denitrificans OCh114
Tang, PloS one 2009 - “...(phosphoenolpyruvate carboxykinase, RD1_1376), ppc (phosphoenolpyruvate carboxylase RD1_4248), ppdK (pyruvate phosphate dikinase, RD1_1948), aatA (aspirate aminotransferase, RD1_3892), tme (malic enzyme, RD1_0421) and ilvA (threonine deaminase, RD1_0416). Relative gene expression value of each gene is calculated with 2 (Ct) , where Ct=Ct target gene Ct 16S rRNA gene...”
BBMN68_1544 pyridoxal phosphate-dependent aminotransferase from Bifidobacterium longum subsp. longum BBMN68
35% identity, 91% coverage
- Mechanism analysis of acid tolerance response of bifidobacterium longum subsp. longum BBMN 68 by gene expression profile using RNA-sequencing
Jin, PloS one 2012 - “...2.37 * E COG0436 Aspartate/tyrosine/aromatic aminotransferase BBMN68_88 71 167.86 2.36 * E COG0436 Aspartate/tyrosine/aromatic aminotransferase BBMN68_1544 245 572.85 2.34 * E COG0436 Aspartate/tyrosine/aromatic aminotransferase BBMN68_1747 122.62 299.33 2.44 * E COG0683 livK K01999, branched-chain amino acid transport system substrate-binding protein BBMN68_1748 194.66 173.56 1.12 E COG0559...”
- “...more than 2 folds ( Table 4 ). BBMN68_1775 , BBMN68_1595 , BBMN68_88 , and BBMN68_1544 encoding PLP-dependent aspartate, tyrosine, and aromatic aminotransferase were differential gene expression showed ( Table 4 ). By analyzing the metabolism pathways, it was known that aspartate metablism provided the precursors...”
Tery_0293 aminotransferase, class I and II from Trichodesmium erythraeum IMS101
31% identity, 92% coverage
- The use of genome-scale metabolic network reconstruction to predict fluxes and equilibrium composition of N-fixing versus C-fixing cells in a diazotrophic cyanobacterium, Trichodesmium erythraeum
Gardner, BMC systems biology 2017 - “...transaminase Leptolyngbya sp. NIES 3755 L-aspartase 4.3.1.1 Tery_1328 Fumarase Nitrosococcus oceani L-arogenate: 2-oxoglutarate aminotransferase 2.6.1.79 Tery_0293 L-aspartate aminotransferase Pleurocapsa sp. PCC 7327 L-threonine ammonium-lyase 4.3.1.19 Tery_4742 Pyridoxal-5-phosphate-dependent enzyme, beta subunit/cysteine synthase A Zymomonas mobilis subsp. NRRL B-12526 Isoprenoid Synthesis Tocopherol phytyltransferase 2.5.1.117 Tery_3881 Homogentisate phytyltransferase Nostoc...”
- “...Tery_4268 2,3-Dimethylmalate lyase/methylisocitrate lyase Candidatus Nitrososphaera gargensis Assumed Promiscuous Amino Acid Metabolism 4-Hydroxyglutamate transaminase 2.6.1.23 Tery_0293 L-aspartate aminotransferase T. erythraeum Cofactor and Energy Carrier Metabolism Dihydroneopterin P i dephosphorylase 3.6.1.1 Tery_1519 Inorganic diphosphatase Dihydroneopterin PPP i dephosphorylase 3.6.1.1 Tery_1519 Inorganic diphosphatase Lipid Metabolism/Secondary Carbon Metabolism Glycoaldehyde...”
LOC123915658 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase from Trifolium pratense
34% identity, 75% coverage
- Expression and Variation of the Genes Involved in Rhizobium Nodulation in Red Clover
Dinkins, Plants (Basel, Switzerland) 2022 - “...protein ATS3B-like; NPD LOC123884281 5.08 20,011.87 11.95 ctenidin-1-like LOC123916883 6.72 17,961.34 11.38 NMS32/34 protein, putative LOC123915658 3049.67 13,975.22 2.20 bifunctional aspartate aminotransferase LOC123893140 4110.65 13,859.39 1.75 legume-specific_protein LOC123918940 4.23 13,807.55 11.67 NMS32/34 protein, putative LOC123891069 18.14 13,225.90 9.51 leghemoglobin_Lb120-1 LOC123888452 57.99 12,010.79 7.69 early_nodulin_ENOD18 LOC123913320 2502.68...”
GRMZM2G067265 uncharacterized protein LOC100276369 from Zea mays
36% identity, 70% coverage
- Soil chemistry determines whether defensive plant secondary metabolites promote or suppress herbivore growth
Hu, Proceedings of the National Academy of Sciences of the United States of America 2021 - “...database under the following accession numbers ZmActin (GRMZM2G126010), ZmMPI (GRMZM2G028393), ZmRIP2 (GRMZM2G119705), ZmRPI (GRMZM2G035599), ZmIDI4 (GRMZM2G067265), ZmNAS3 (GRMZM2G478568), ZmDMAS1 (GRMZM2G060952), ZmTOM2 (GRMZM5G877788), ZmYS1 (GRMZM2G156599), ZmNRAMP1 (GRMZM2G178190) and ZmIRO2 (GRMZM2G057413). We thank Professor Nicolaus von Wirn from Institut fr Pflanzengenetik und Kulturpflanzenforschung Gatersleben for sharing the ys1...”
- Transcriptomic analyses of maize ys1 and ys3 mutants reveal maize iron homeostasis
Nozoye, Genomics data 2015 - “...ACAAACTTGGGGACAGGCCA ACTCTAACTCTCTGAGAAGA ZmMTK GRMZM2G464137 CCTGCCTGATATATACAACA CTTGCTGGCATCCTTGATTG ZmIDI2 GRMZM2G139533 CTCTTCATTCGGGGGGAGTT GGAGAGATCAATGGAAGTTA ZmFDH GRMZM2G049811 CTGATTCTGCTCAGGAACTT TCTCCGTAAGAGGTGTGTTG ZmIDI4 GRMZM2G067265 CTGAGGCAATAGCTGCAACC AGACCTCATCTGAGAACAGC ZmRPI GRMZM2G035599 GGCGTCGTCGAGCACGGCAT CTTCCTTTCCATGACTGCGA ZmPRPPs GRMZM2G065030 GACCTCCGTTTGAGGTTCTG CGAGCATCGACAGTAAGTAC ZmIRO2 GRMZM2G057413 AACGACCTCTACTCCTCGCT CTGCAGCTCCGGGATGTACT ZmIRO3 GRMZM2G350312 AGATTCATAAGGCTGAGAGG TCGAAGAGAGTCTACTTGAA ZmNAS1 GRMZM2G034956 ATCCTGAGGACATTCGTCGC CAGCGATTTGTAACTATTAT ZmNAS3 GRMZM2G478568 CGTGTCTACACCACATGCGT GAGCTAAGCTACATGCTAA ZmDMAS1 GRMZM2G060952 CTGATCGTGAAGAGCTTCGA GCAGGGCAGTGGCACGCATT...”
- Comparative transcriptomics uncovers alternative splicing changes and signatures of selection from maize improvement
Huang, BMC genomics 2015 - “...and developmental process regulation [ 35 ]. GRMZM2G088064 encodes alanine aminotransferase, and both GRMZM2G094712 and GRMZM2G067265 encode aspartate aminotransferases; all are induced by stress factors that facilitate the acquisition of somatic embryogenesis capacities [ 36 ]. GRMZM2G002656, AC230011.2_FG002, and seven other genes encode proteins with an...”
- “...these genes play potential roles in various biotic and abiotic stress responses. Moreover, GRMZM2G094712, GRMZM2G088064, GRMZM2G067265, and 11 other genes are involved in oxygenic photosynthesis; GRMZM2G382914, GRMZM2G003724, GRMZM2G089136, and nine other genes are involved in the CalvinBensonBassham cycle. Some flowering-related genes also showed AS level increase...”
- Characterizing the crucial components of iron homeostasis in the maize mutants ys1 and ys3
Nozoye, PloS one 2013 - “...GRMZM2G171111; ZmAPT , GRMZM2G093347; ZmMTK , GRMZM2G464137; ZmIDI2 , GRMZM2G139533; ZmFDH , GRMZM2G049811; ZmIDI4 , GRMZM2G067265; ZmRPI , GRMZM2G035599) and MAs biosynthesis ( ZmNAS1 , GRMZM2G034956; ZmDMAS1 , GRMZM2G060952) were markedly elevated in roots under Fe-deficient conditions ( Figure 3 ). The expression level of ZmNAS3...”
- “...GRMZM2G171111; ZmAPT , GRMZM2G093347; ZmMTK , GRMZM2G464137; ZmIDI2 , GRMZM2G139533; ZmFDH , GRMZM2G049811; ZmIDI4 , GRMZM2G067265; ZmRPI , GRMZM2G035599; ZmPRPPs , GRMZM2G065030), transcription ( ZmIRO2 , GRMZM2G057413; ZmIRO3 , GRMZM2G350312), MAs biosynthesis ( ZmNAS1 , GRMZM2G034956; ZmNAS3 , GRMZM2G478568; ZmDMAS1 , GRMZM2G060952), and transport ( ZmYS1...”
Q25C96 Putative asparate aminotransferase from Hordeum vulgare
34% identity, 83% coverage
CSP5_0812 pyridoxal phosphate-dependent aminotransferase from Cuniculiplasma divulgatum
30% identity, 94% coverage
- Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, Cuniculiplasma divulgatum
Bargiela, Microorganisms 2020 - “...-lyase (CSP5_0202, up 1.16 fold), threonine synthase (CSP5_1396, 1.49-fold), homoserine dehydrogenase (CSP5_0597, 1.18-fold), aspartate aminotransferase (CSP5_0812, 1.24-fold) and aspartate ammonia-lyase (CSP5_1895, 1.14-fold)). We expect that overexpression of methionine production is required for the synthesis of SAM (S-adenosylmethionine). SAM synthetase is one of the most expressed proteins...”
A0A1D6I5Q5 L-glutamine-4-(methylsulfanyl)-2-oxobutanoate aminotransferase (EC 2.6.1.117) from Zea mays (see paper)
36% identity, 62% coverage
AAT_SYNY3 / Q55128 Aspartate aminotransferase; AAT; AspAT; EC 2.6.1.1 from Synechocystis sp. (strain ATCC 27184 / PCC 6803 / Kazusa) (see 2 papers)
sll0402 aspartate aminotransferase from Synechocystis sp. PCC 6803
35% identity, 86% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:24302739, PubMed:25070637). Has very weak prephenate aminotransferase activity (PubMed:25070637).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms
Graindorge, The Journal of biological chemistry 2014 - “...following UniProt accession numbers: Q02635, A3PMF8, P54691, Q55128, Q55828, Q3AJX2, Q82IK5, Q82DR2, Q82JN7, Q82EU6, O50434, Q82WA8, Q82UJ8, Q82S89, and Q82TJ4....”
- Arginine inhibits the arginine biosynthesis rate-limiting enzyme and leads to the accumulation of intracellular aspartate in Synechocystis sp. PCC 6803
Katayama, Plant molecular biology 2024 - “...respectively (Fig. 2 c). The expression levels of the gene encoding argD (slr1022) and aspC (sll0402) were not significantly different among the GT, ArgGOX, and ArgHOX strains (Fig. 2 c). We also measured RNA expression of the argB gene encoding NAGK that is a significant regulator...”
- Metabolic engineering of Synechocystis sp. PCC 6803 for the improved production of phenylpropanoids
Kukil, Microbial cell factories 2024 - “...ago [ 14 ]. In Synechocystis, two branched-chain aminotransferases encoded by slr0032 (UniprotKB P54691) and sll0402 ( aspC , NCBI accession no WP_010873487.1), annotated as aspartate aminotransferases, were purified as recombinant proteins and demonstrated very little PPA-AT activity, while some aromatic aminotransferase activity was detected using...”
- Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism
Baers, Plant physiology 2019 - “...biosynthetic steps under these conditions. For example, only one of the two possible Asp aminotransferases (Sll0402) was detected. The remaining proteome may not have been detected for a variety of reasons. Only proteins that were identified in both replicates were included, and, although MS is a...”
ZMO0342 aminotransferase class I and II from Zymomonas mobilis subsp. mobilis ZM4
32% identity, 92% coverage
1j32A / Q8RR70 Aspartate aminotransferase from phormidium lapideum
aspC / BAB86290.1 aspartate aminotransferase from Phormidium lapideum (see paper)
32% identity, 92% coverage
- Ligand: pyridoxal-5'-phosphate (1j32A)
NSCAC_1014 pyridoxal phosphate-dependent aminotransferase from Candidatus Nitrosacidococcus tergens
32% identity, 92% coverage
- Ammonia oxidation at pH 2.5 by a new gammaproteobacterial ammonia-oxidizing bacterium
Picone, The ISME journal 2021 - “...most likely replenished by phosphoenolpyruvate carboxylase (EC 4.1.1.31, NSCAC_1317) and subsequently used for L-aspartate biosynthesis (NSCAC_1014, 1244). From L-aspartate, fumarate can be formed via either adenylo- or L-arginino-succinate intermediates (NSCAC_0583, 0584, 1629, 1756). Together with the fumarate formed here, L-aspartate can also be used to form...”
HMPREF0424_1220 putative aspartate transaminase from Gardnerella vaginalis 409-05
34% identity, 87% coverage
- Comparative transcriptomic analysis of Gardnerella vaginalis biofilms vs. planktonic cultures using RNA-seq
Castro, NPJ biofilms and microbiomes 2017 - “...Phosphoglyceratemutase 3.62 Rank Upregulated 1 HMPREF0424_0510 Uncharacterized protein 14.41 2 HMPREF0424_0563 Pyroglutamyl-peptidase I 9.57 3 HMPREF0424_1220 Aminotransferase, class I/II 6.74 4 HMPREF0424_0420 LPXTG-motif cell wall anchor domain-containing protein 6.30 5 HMPREF0424_0397 Uncharacterized protein 5.76 6 HMPREF0424_0573 LysM domain-containing protein 5.51 7 HMPREF0424_0943 ComEA protein 5.15 8...”
- “...function related to hydrolase activity. Furthermore, in biofilm cells we found an overexpression of the HMPREF0424_1220 gene encoding an aminotransferase involved in amino acid biosynthesis. A similar trend was reported for Neisseria meningitides . 18 Interestingly, we found HMPREF0424_0420 , a gene that encodes the LPXTG-motif...”
E5LBC4 Prephenate aminotransferase from Solanum lycopersicum
32% identity, 76% coverage
- Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast⁻Chloroplast Proteomics.
Zhao, International journal of molecular sciences 2019 - “...GO:0005622; GO:0006412; GO:0009570; GO:0009941 K4BXX3 Alpha-1,4 glucan phosphorylase GO:0005737; GO:0005980; GO:0008184; GO:0030170 K4BLP5 Uncharacterized protein E5LBC4 Prephenate aminotransferase GO:0008483; GO:0009058; GO:0030170 Q6T7F1 Phosphoribosylaminoimidazole carboxylase GO:0004638; GO:0005524; GO:0006189; GO:0046872 K4C5B9 Uroporphyrinogen decarboxylase GO:0004853; GO:0005829; GO:0006782; GO:0006783; GO:0009570; GO:0009735; GO:0009941 K4BJL2 Uncharacterized protein GO:0003735; GO:0005840; GO:0006412 K4CPX9 Uncharacterized...”
Cj0762c aspartate aminotransferase from Campylobacter jejuni subsp. jejuni NCTC 11168
CJSA_0718 pyridoxal phosphate-dependent aminotransferase from Campylobacter jejuni subsp. jejuni IA3902
YP_002344169 aspartate aminotransferase from Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
31% identity, 90% coverage
- Metabolic characterisation of Magnetospirillum gryphiswaldense MSR-1 using LC-MS-based metabolite profiling
Abdelrazig, RSC advances 2020 - “...(aspA) mutant of Campylobacter jejuni (unable to utilise any amino acid except serine) and a Cj0762c (aspB) mutant (unable to utilise glutamate), were severely impaired. Recently, microaerobic growth conditions were found to upregulate amino acid synthesis genes in E. coli . 41 Our findings, therefore, may...”
- The Involvement of the Cas9 Gene in Virulence of Campylobacter jejuni
Shabbir, Frontiers in cellular and infection microbiology 2018 - “...the basis of their role in virulence and differential expression in RNA-Seq results. These included Cj0762c (aspartate aminotransferase), Cj1523c (CRISPR-associated protein), Cj1222c (two-component sensor histidine kinase), Cj0996 (GTP cyclohydrolase II), Cj0725c (molybdenum cofactor biosynthesis), Cj0454c (membrane protein), and Cj0330c (50S ribosomal protein L32). For RT-qPCR, a...”
- “...0.0093 Cj1587c Putative ABC transporter 2.70 0.039 0.544 Verification by RT-qPCR Seven genes ( Cj1523c, Cj0762c, Cj1222c, Cj0996, Cj0725c, Cj0454c, Cj0330c ) encoding proteins related to the virulence mechanism of C. jejuni NCTC1168 were selected for validation of RNA-Seq results. Among the seven selected genes only...”
- Convergent Amino Acid Signatures in Polyphyletic Campylobacter jejuni Subpopulations Suggest Human Niche Tropism
Méric, Genome biology and evolution 2018 - “...in the maf6-Cj1347 genomic region (46) cj1253 pnp 472 Polynucleotide phosphorylase/polyadenylase J Translation 7 5 cj0762c aspB 285 Aspartate aminotransferase E Amino acid transport and metabolism genes 6 1 A aspB mutant is defective for entry into cultured human epithelial cells (38) cj0810 nadE 301 NAD...”
- Comparison of Proteomics Profiles of Campylobacter jejuni Strain Bf under Microaerobic and Aerobic Conditions
Rodrigues, Frontiers in microbiology 2016 - “...by glutamine synthetase GlnA (Cj0699c) or also used as substrate of the aspartate:glutamate transaminase AspB (Cj0762c) to produce aspartate and -ketoglutarate from oxaloacetate and glutamate (Guccione et al., 2008 ). Moreover, glutamate catabolism and the utilization of aspartate are crucial for the metabolic fitness and growth...”
- Metabolomic analysis of the food-borne pathogen Campylobacter jejuni: application of direct injection mass spectrometry for mutant characterisation
Howlett, Metabolomics : Official journal of the Metabolomic Society 2014 - “...being 2-oxoglutarate, a glutamate precursor, malate and citrate AspB in C. jejuni is encoded by cj0762c and is essential for growth on glutamate, as it converts glutamate to aspartate using oxaloacetate as the amino-acceptor. In the aspB mutant a significant accumulation of m/z 146.00 (glutamate) is...”
- “...physiologically significant as a cj0150c mutant strain shows no growth defect on glutamate, whereas a cj0762c ( aspB ) mutant is completely unable to grow on or utilise glutamate or proline as a carbon source (Guccione et al. 2008 ). Here, we compared the metabolic fingerprints...”
- Defining the metabolic requirements for the growth and colonization capacity of Campylobacter jejuni
Hofreuter, Frontiers in cellular and infection microbiology 2014 - “...the type I glutamine synthetase GlnA (Cj0699c) or is substrate of the aspartate:glutamate transaminase AspB (Cj0762c) catalyzing the generation of aspartate and 2-oxoglutarate from oxaloacetate and glutamate (Guccione et al., 2008 ). AspB plays an important role in the intermediary metabolism of C. jejuni and its...”
- Nutrient acquisition and metabolism by Campylobacter jejuni
Stahl, Frontiers in cellular and infection microbiology 2012 - “...al., 2006 ). Once glutamate has been taken up into the cell, the enzyme AspB (Cj0762c), allows for the transamination of glutamate to aspartate. One molecule of glutamate and oxaloacetate are used to produce aspartate and 2-oxoglutarate in a reversible reaction (Guccione et al., 2008 )....”
- Hyperosmotic stress response of Campylobacter jejuni
Cameron, Journal of bacteriology 2012 - “...synthase glnA aspB leuD leuC leuB leuA cj0699c cj0762c cj1716c cj1717c cj1718c cj1719c November 2012 Volume 194 Number 22 mqo icd mdh sucC sucD...”
- More
- Small RNA CjNC110 regulates the activated methyl cycle to enable optimal chicken colonization by Campylobacter jejuni
Ruddell, mSphere 2025 - “...1824 2.06 W7 only RNAup hom CJSA_0140 Cj0149c 5 5258 1723 6.42 Both IntaRNA aspB CJSA_0718 Cj0726c 5 5258 4854 4.96 Both IntaRNA heuR CJSA_1321 Cj1387c 5 713 6470 3.01 Both RNAup metC CJSA_1324 Cj1392 5 6669 1821 4.35 Both RNAup Transport metQ3 a2 CJSA_0726 Cj0770c...”
- Amino acid-dependent growth of Campylobacter jejuni: key roles for aspartase (AspA) under microaerobic and oxygen-limited conditions and identification of AspB (Cj0762), essential for growth on glutamate.
Guccione, Molecular microbiology 2008 (PubMed)- GeneRIF: The aspA mutant and the AspB mutant were severely growth impaired in complex media.
PAT_PETHY / E9L7A5 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase; PhPPA-AT; EC 2.6.1.1; EC 2.6.1.78; EC 2.6.1.79 from Petunia hybrida (Petunia) (see 2 papers)
33% identity, 75% coverage
- function: Prokaryotic-type aspartate aminotransferase. Has also a prenate transaminase activity (PubMed:21102469). Involved in the aromatic amino acids biosynthesis pathway via the arogenate route (PubMed:21102469). Required for the transamination of prephenate into arogenate (PubMed:21102469). Can use 2-oxoglutarate, oxaloacetate and prephenate as substrates, but not phenylpyruvate or 4- hydroxyphenylpyruvate (PubMed:21102469).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + oxaloacetate = prephenate + L-aspartate (RHEA:20445)
catalytic activity: L-arogenate + 2-oxoglutarate = prephenate + L-glutamate (RHEA:22880)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Bioinformatics-based Characterization of the Sequence Variability of Zika Virus Polyprotein and Envelope Protein (E).
Polanco, Evolutionary bioinformatics online 2022 - “...Q9X3Y6, P05652, O50627, and GW. CAM-E Q9S850, Q9X3Y6, and GW. Q32-E SVTP, and GW. POL-P E9L7A5, A0A142I5B9, P24230, POL-P, Q9MA50, Q8MI68, A8MLD3, B6HJU4, P0C988, P50569, Q5QMN5, Q9MYY0, Q0CBN5, Q7VPJ7, A5EXM7, Q5BJH7, Q7UH14, Q0BV25, P0C987, Q65215, Q8XD86, Q9GKL2, Q13B10, Q9ZBD8, E9D269, Q8ZRP8, P53678, P97784, P53677, P75464, Q32Q86,...”
CJJ81176_0783 aspartate aminotransferase from Campylobacter jejuni subsp. jejuni 81-176
31% identity, 90% coverage
D820_RS03740 pyridoxal phosphate-dependent aminotransferase from Streptococcus mutans ATCC 25175
32% identity, 92% coverage
LBA0856 aspartate aminotransferase from Lactobacillus acidophilus NCFM
34% identity, 89% coverage
- Lactobacillus acidophilus-Rutin Interplay Investigated by Proteomics
Mazzeo, PloS one 2015 - “...metabolism DOWN(-1.7) isomerase LBA0651 Pentose phosphate pathway UP(1.6) AMINO ACIDS BIOSYNTESIS AND METABOLISM aspartate aminotransferase LBA0856 Lysine biosynthesis UP(1.6) branched-chain amino acid aminotransferase ilvE LBA1341 Valine, leucine and isoleucine biosynthesis or degradation UP(1.6) glutamine synthetase glnA LBA1501 Alanine, aspartate and glutamate metabolism / Arginine and proline...”
SCO4645 aspartate aminotransferase from Streptomyces coelicolor A3(2)
34% identity, 88% coverage
lmo1897 similar to aspartate aminotransferases from Listeria monocytogenes EGD-e
31% identity, 92% coverage
LGG_00828 aromatic amino acid aminotransferase / Acetyldiaminopimelate aminotransferase from Lactobacillus rhamnosus GG
33% identity, 91% coverage
aspC / Q4FNY9 alanine—glyoxylate transaminase (EC 2.6.1.44) from Pelagibacter ubique (strain HTCC1062) (see 2 papers)
SAR11_0279 probable aspartate transaminase from Candidatus Pelagibacter ubique HTCC1062
28% identity, 97% coverage
SPD_1373 aspartate aminotransferase from Streptococcus pneumoniae D39
32% identity, 91% coverage
- The LuxS/AI-2 Quorum-Sensing System of Streptococcus pneumoniae Is Required to Cause Disease, and to Regulate Virulence- and Metabolism-Related Genes in a Rat Model of Middle Ear Infection
Yadav, Frontiers in cellular and infection microbiology 2018 - “...49 SPD_1357 ( aliB ) Oligopeptide ABC transporter, oligopeptide-binding protein AliB Transmembrane transport 3.78 50 SPD_1373 ( aspC ) Aminotransferase Biosynthetic process 166 51 SPD_1381 ( def-2 ) Peptide deformylase Translation 5.55 52 SPD_1626 ( xth ) Exodeoxyribonuclease III Endonuclease activity 200 53 SPD_1642 ( proWX...”
- Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling
Härtel, The Journal of biological chemistry 2012 - “...used are as follows: aspC, aspartate aminotransferase (SPD_1373 (EC 2.6.1.1)); aroC, chorismate synthase (SPD_1208 (EC 4.2.3.5)); glyA, SHMT (SPD_0910 (EC...”
- “...reaction, the aspartate transaminase (aspC, SPD_1373 (EC 2.6.1.1)) converts [1,2,3-13C3]oxaloacetate into [1,2,3-13C3]Asp and thereby unlabeled glutamate...”
SP_1544 aspartate aminotransferase from Streptococcus pneumoniae TIGR4
32% identity, 91% coverage
SAR11_0080 aspartate transaminase from Candidatus Pelagibacter ubique HTCC1062
29% identity, 90% coverage
BBIF_0278 pyridoxal phosphate-dependent aminotransferase from Bifidobacterium bifidum S17
34% identity, 95% coverage
- Proteomic Profiling of Bifidobacterium bifidum S17 Cultivated Under In Vitro Conditions
Wei, Frontiers in microbiology 2016 - “...proteome of B. bifidum S17. Locus_tag Gene name Description Length [aa] a MW [kDa] b BBIF_0278 aspC1 Aminotransferase 401 42.8 BBIF_0311 yhdR Aspartate aminotransferase 396 43.2 BBIF_0342 bbif_0342 Multiple substrate aminotransferase (MsaT) containing domain of GntR family (transcriptional regulator) 509 55.6 BBIF_0469 bbif_0469 Aspartate/tyrosine/aromatic aminotransferase 398...”
XNR_3703 pyridoxal phosphate-dependent aminotransferase from Streptomyces albidoflavus
34% identity, 88% coverage
- Insights into naturally minimised Streptomyces albus J1074 genome
Zaburannyi, BMC genomics 2014 - “...major glutamine synthetase (GS), GlnA (XNR_4684), NAD-specific glutamate dehydrogenase GDH (XNR_1879), and aspartate aminotransferase AspC (XNR_3703) were maintained at high levels up to the 60h time point. While S. coelicolor has 5 GS-like genes, S. albus J1074 contains four genes for glutamine synthetase: XNR_4684 , XNR_4658...”
NOAT_STRMU / Q8DTM1 Asparagine--oxo-acid transaminase; Asparagine:2-oxoglutarate aminotransferase; EC 2.6.1.14 from Streptococcus mutans serotype c (strain ATCC 700610 / UA159) (see paper)
Q8DTM1 asparagine-oxo-acid transaminase (EC 2.6.1.14) from Streptococcus mutans serotype c (see paper)
31% identity, 92% coverage
- function: Catalyzes the transamination reaction between L-asparagine and 2-oxoglutarate to produce L-glutamate and 2-oxosuccinamate (PubMed:22569339). Is not active with pyruvate as amine acceptor (PubMed:22569339). May also use other amino acids as substrates.
catalytic activity: a 2-oxocarboxylate + L-asparagine = 2-oxosuccinamate + an L- alpha-amino acid (RHEA:19813)
catalytic activity: L-asparagine + 2-oxoglutarate = 2-oxosuccinamate + L- glutamate (RHEA:52528)
cofactor: pyridoxal 5'-phosphate - Profiling the orphan enzymes.
Sorokina, Biology direct 2014 - “...in the degradation process via the histidine transaminase activity. (3) the candidate protein (UniProt AC Q8DTM1) shares more than 50% of amino acid identity with biochemically characterized aspartate aminotransferases (UniProt ACs P23034, Q59228). This activity is more coherent with the asparaginyl tRNA synthetase genomic context than...”
- Prediction and identification of sequences coding for orphan enzymes using genomic and metagenomic neighbours
Yamada, Molecular systems biology 2012 - “...; Stahl and Stintzi, 2011 ). Heterologous expression of candidate genes For EC 2.6.1.14, protein Q8DTM1 (STRING id) was PCR amplified from Streptococcus mutans (DSM 20523) gDNA. For EC 2.6.1.38, protein Q8R5Q4 (STRING id) was PCR amplified from Thermoanaerobacter tengcongensis (DSM 15242) gDNA (see Supplemental Methods...”
SSIG_RS12365 pyridoxal phosphate-dependent aminotransferase from Streptomyces filamentosus NRRL 11379
34% identity, 89% coverage
GSU1242 aspartate aminotransferase from Geobacter sulfurreducens PCA
30% identity, 96% coverage
FTN_1146 aspartate aminotransferase from Francisella tularensis subsp. novicida U112
31% identity, 89% coverage
RSp0943 PROBABLE ASPARTATE AMINOTRANSFERASE A PROTEIN from Ralstonia solanacearum GMI1000
34% identity, 88% coverage
Q47LH8 Aminotransferase from Thermobifida fusca (strain YX)
32% identity, 89% coverage
BF638R_RS02825 pyridoxal phosphate-dependent aminotransferase from Bacteroides fragilis 638R
32% identity, 91% coverage
- Genome-scale metabolic modeling of the human gut bacterium Bacteroides fragilis strain 638R
Neal, PLoS computational biology 2023 - “...supplemented with amino acids. 10.1371/journal.pcbi.1011594.t003 Table 3 Differentially Essential Genes. Gene Identifier Function Metabolites Blocked BF638R_RS02825 Aminotransferase All 8 NTPs and dNTPs; NADH/NADPH; Several amino acids BF638R_RS14560 Methionine synthase 6 amino acids BF638R_RS19065 Methylenetetrahydrofolate reductase Arginine, histidine, proline BF638R_RS02840 Homoserine O-succinyltransferase Arginine, histidine, proline Each row...”
spr0035 Aspartate aminotransferase from Streptococcus pneumoniae R6
33% identity, 89% coverage
ZP_01264754 aspartate transaminase from Candidatus Pelagibacter ubique HTCC1002
29% identity, 90% coverage
- Molecular cloning and heterologous expression of the dehydrophos biosynthetic gene cluster
Circello, Chemistry & biology 2010 - “...Aciduliprofundum boonei malate/ l -lactate dehydrogenase (YP_002578914) 130/367(35%) dhpD 397 Candidatus Pelagibacter ubique aspartate transaminase (ZP_01264754) 112/403 (28%) dhpE 296 Frankia alni putative PEP phosphomutase (YP_716511) 147/275 (53%) dhpF 381 Amycolatopsis orientalis putative phosphonopyruvate decarboxylase (CAB45023) 182/371(49%) dhpG 396 Amycolatopsis orientalis putative alcohol dehydrogenase (CAB45024) 152/367(41%)...”
LOC105033000 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase from Elaeis guineensis
31% identity, 76% coverage
- Developing functional markers for vitamin E biosynthesis in oil palm
Dou, PloS one 2021 - “...EgPK-2 AtPK 3E-70 Scaffold - LOC105036025 6 539 XP_010910062.1 307 EgTAT-2 AtTAT2 5E-22 Scaffold - LOC105033000 2 850 XP_010905916.1 474 EgTAT-3 AtTAT2 2E-16 chr1 + LOC105050532 9 153 XP_010928898.1 481 EgTAT-4 AtTAT2 2E-24 chr2 - LOC105037948 4 940 XP_010911890.1 412 EgTAT-5 AtTAT2 0 chr3 + LOC105040940...”
PAT_ARATH / Q9SIE1 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase; AtAAT; AtPPA-AT; Protein MATERNAL EFFECT EMBRYO ARREST 17; EC 2.6.1.1; EC 2.6.1.78; EC 2.6.1.79 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
Q9SIE1 aspartate transaminase (EC 2.6.1.1); aspartate-prephenate aminotransferase (EC 2.6.1.78); glutamate-prephenate aminotransferase (EC 2.6.1.79) from Arabidopsis thaliana (see 4 papers)
AT2G22250 aminotransferase class I and II family protein from Arabidopsis thaliana
31% identity, 75% coverage
- function: Prokaryotic-type aspartate aminotransferase. Has also a prenate transaminase activity. Involved in the aromatic amino acids biosynthesis pathway via the arogenate route. Required for the transamination of prephenate into arogenate. Required for early development of the embryo.
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + oxaloacetate = prephenate + L-aspartate (RHEA:20445)
catalytic activity: L-arogenate + 2-oxoglutarate = prephenate + L-glutamate (RHEA:22880)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - The Shared Proteome of the Apomictic Fern Dryopteris affinis ssp. affinis and Its Sexual Relative Dryopteris oreades
Ojosnegros, International journal of molecular sciences 2022 - “...10 175 Reproduction 135880-210_3_ORF2 Q8LD94 CYP5 Glucan endo-1,3-beta-glucosidase, acidic isoform 25 251 0 Reproduction 152971-191_2_ORF2 Q9SIE1 PAT Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase 55 778 0 Reproduction 116951-229_1_ORF2 F4IFC5 EMB2761 Threonine--tRNA ligase, chloroplastic/mitochondrial 2 45 625 0 Reproduction 76060-289_2_ORF2 Q9CAE3 FLD Protein flowering locus D 52...”
- Identification and biochemical characterisation of tyrosine aminotransferase from Anthoceros agrestis unveils the conceivable entry point into rosmarinic acid biosynthesis in hornworts
Busch, Planta 2021 - “...related aminotransferases were added: A. thaliana TrpAT (AAO63403), A. thaliana IPAAT (AAP68293), A. thaliana PrephenAT (Q9SIE1), Oryza sativa AlaAT (BAA77261), A. thaliana AlaAT (Q9SR86), A. thaliana Ala/GluAT (AAN62333), Glycine max AspAT (AAC50015), A. thaliana (mitochondrial) AspAT1 (P46643), A. thaliana (cytoplasmic) AspAT2 (P46645), A. thaliana (plastidic) AspAT5...”
- “...related aminotransferases (open squares): Arabidopsis thaliana TrpAT (AAO63403), A. thaliana IPAAT (AAP68293), A. thaliana PrephenAT (Q9SIE1), Oryza sativa AlaAT (BAA77261), A. thaliana AlaAT (Q9SR86), A. thaliana Ala/GluAT (AAN62333), Glycine max AspAT (AAC50015), A. thaliana (mitochondrial) AspAT1 (P46643), A. thaliana (cytoplasmic) AspAT2 (P46645), A. thaliana (plastidic) AspAT5...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase 30 Q9SQT8, At3g06350 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase 64, 66 Q9SIE1, At2g22250 Branched-chain-amino-acid aminotransferase 3 64, 66 Q9M401, At3g49680 Chorismate synthase 71, 73 P57720, At1g48850 Cysteine synthase 82, 83, 102 P47999, At2g43750 D-3-phosphoglycerate dehydrogenase 1 18, 2224, 30, 4547, 54, 55...”
- Involvement of a universal amino acid synthesis impediment in cytoplasmic male sterility in pepper
Fang, Scientific reports 2016 - “...were derived from the conserved protein sequences of the UniProt accessions Q9C5U8 (HDH), Q9LIR4 (DAD), Q9SIE1 (ATAAT), P47999 (CS), P54887 (P5CS), Q9LV03 (GS), and CAC80377 (GAPDH). Each peptide, along with an additional N-terminal cysteine (not part of the protein sequence), was synthesized and purified on a...”
- “...ATP-dependent zinc metalloprotease FTSH 8 129.67 2 685 5.17 65.61 1 1.130.12 2.530.21 1.730.16 0.490.21 Q9SIE1 Bifunctional aspartate aminotransferase 429.34 5 475 5.97 45.36 1 1.530.11 1.090.25 0.770.12 0.620.18 Q94AW8 Chaperone protein dnaJ 3 54.22 2 420 5.76 46.12 1 1.730.19 1.820.15 0.860.24 0.340.04 P21238 Chaperonin...”
- Transcription factor OsMYB2 triggers amino acid transporter OsANT1 expression to regulate rice growth and salt tolerance
Nie, Plant physiology 2025 (no snippet) - Genome-Wide Association and RNA-Seq Analyses Reveal a Potential Candidate Gene Related to Oil Content in Soybean Seeds
Jia, International journal of molecular sciences 2024 - “...Glyma.20G208400 6.94 AT1G62510 Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein E3 rs33193693 Glyma.11G238300 3.64 AT2G22250 Aspartate aminotransferase E3 rs18054299 Glyma.09G099500 3.54 AT1G16310 Cation efflux family protein E3 rs18196571 Glyma.17G174700 3.07 AT5G09910 Ras-related small GTP-binding family protein Note: E1: Nenjiang, E2: Beian, E3: five connected lakes....”
- Rewiring of primary metabolism for ammonium recycling under short-term low CO2 treatment - its implication for C4 evolution
Miao, Frontiers in plant science 2024 - “...dikinase regulatory protein (PPDK-RP, AT4G21210), alanine aminotransferase (AlaAT1, AT1G17290), and aspartate aminotransferase (AspAT, AT4G31990, AT5G19550, AT2G22250) were also upregulated ( Figure1 ; Supplementary Dataset S1 ). In addition, the genes encoding C 4 related transporters were upregulated significantly, which includes phosphoenolpyruvate/phosphate translocator (PPT1, AT3G01550; PPT2, AT5G33320),...”
- Accumulation of the Auxin Precursor Indole-3-Acetamide Curtails Growth through the Repression of Ribosome-Biogenesis and Development-Related Transcriptional Networks
Sánchez-Parra, International journal of molecular sciences 2021 - “...], utilizing the 1.7 crystal structure of a bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase (PAT, At2g22250) deposited in the Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Base (PDB: 6F5V) [ 30 ]. In accordance with [ 29 ], we used the PAT structure as a...”
- The entry reaction of the plant shikimate pathway is subjected to highly complex metabolite-mediated regulation
Yokoyama, The Plant cell 2021 - “...enzymatic assay For arogenate production, prephenate was enzymatically converted into arogenate by Arabidopsis prephenate aminotransferase (AT2G22250) recombinant enzyme and purified by an anion-exchange chromatography, as previously described ( Maeda et al., 2010 ; Schenck et al., 2015 ). For treatment of AthCM2 (AT5G10870) recombinant protein to...”
- Gel-based proteomic map of Arabidopsis thaliana root plastids and mitochondria
Grabsztunowicz, BMC plant biology 2020 - “...3-dehydroquinate dehydratase/shikimate dehydrogenase 30 Q9SQT8, At3g06350 Bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase 64, 66 Q9SIE1, At2g22250 Branched-chain-amino-acid aminotransferase 3 64, 66 Q9M401, At3g49680 Chorismate synthase 71, 73 P57720, At1g48850 Cysteine synthase 82, 83, 102 P47999, At2g43750 D-3-phosphoglycerate dehydrogenase 1 18, 2224, 30, 4547, 54, 55 O49485,...”
- “...was represented in root plastid samples, as the bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase (PAT-AT; At2g22250) [ 39 ] was identified in spots 64 and 66 (Table 1 , Fig. 3 ). Carbohydrate metabolism and assimilatory pathways Twenty percent of the identified plastidial proteins represented carbohydrate...”
- AtDRO1 is nuclear localized in root tips under native conditions and impacts auxin localization
Waite, Plant molecular biology 2020 - “...B2 127.33 2.70E02 AT1G60460 AT1G60460 Unknown protein 129.52 1.98E02 AT3G62620 AT3G62620 Sucrose-phosphate related 237.57 4.66E02 AT2G22250 AAT Aspartate aminotransferase 334.24 2.63E02 RNA from pooled lateral and primary root tips, from populations of eight 14-day-old atdro1 or WT seedlings, revealed a relatively small number DEGs. 87 DEGs...”
- Intact salicylic acid signalling is required for potato defence against the necrotrophic fungus Alternaria solani
Brouwer, Plant molecular biology 2020 - “...genes (Table 4 ). The homolog of one of these genes (PGSC0003DMG400027919) in A. thaliana (AT2G22250) encodes a prephenate aminotransferase (PAT), involved in the aromatic amino acid pathway. This is an important pathway involved in the biosynthesis of many aromatic secondary metabolites (Graindorge et al. 2010...”
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NP_850022 aspartate aminotransferase from Arabidopsis thaliana
31% identity, 83% coverage
SSU0569 aspartate aminotransferase from Streptococcus suis P1/7
30% identity, 92% coverage
FTT_1165c aspartate aminotransferase from Francisella tularensis subsp. tularensis SCHU S4
31% identity, 89% coverage
EF0891 aspartate aminotransferase, putative from Enterococcus faecalis V583
32% identity, 88% coverage
- Influence of the Alternative Sigma Factor RpoN on Global Gene Expression and Carbon Catabolism in Enterococcus faecalis V583
Keffeler, mBio 2021 - “...performed quantitative real-time PCR (qRT-PCR) on a set of genes that represented both upregulated ( ef0891 and ef2223 ) and downregulated ( ef0019 [ mptB ], ef1017 [ lptB ], and ef3210 [ xpoA ]) genes in the rpoN mutant. This list of validated genes included...”
- “...were found to be unique to the rpoN mutant ( ef1017 , ef3210 , and ef0891 ). We examined the expression of ef0891 and ef2223 in the rpoN mutant by using qRT-PCR to compare transcript abundance to that in the parental and rpoN -complemented strains. The...”
- Drosophila host model reveals new enterococcus faecalis quorum-sensing associated virulence factors
Teixeira, PloS one 2013 - “...4 LemA family protein +3 EF0563 5 Hypothetical protein +3 EF0776 6 Hypothetical protein +11 EF0891 7 Aspartate aminotransferase putative 4 EF0892 Aminoacid ABC transporter,ATP-binding protein 3 EF0893 Aminoacid ABC transporter/permease 3 3 3 EF1097 Putative Bacteriocin +31 +23 +30 +47 EF1218 8 spermidine/putrescine ABC transporter,permease...”
- “...mRNA levels were altered only when both proteases were absent ( ef0411 , ef0563 , ef0891 , ef0892 , ef1218 ); those for which mRNA accumulated only in the presence of both proteases ( ef3193 and ef3194 ) and those affected in the absence of only...”
- Global transcriptional analysis of the stringent response in Enterococcus faecalis
Gaca, Microbiology (Reading, England) 2012 - “...EF0674 EF0675 EF0706 EF0758 EF0759 EF0807 EF0820 EF0867 EF0891 EF0893 EF0910 EF1117 EF1118 EF1119 EF1120 EF1211 EF1405 EF1413 EF1513 EF1584 EF1585 EF1597 EF1672...”
- Comparative genomic analysis of pathogenic and probiotic Enterococcus faecalis isolates, and their transcriptional responses to growth in human urine
Vebø, PloS one 2010 - “...An operon comprising a putative amino acid ABC transporter (EF0893-92) and a putative aspartate aminotransferase (EF0891) was highly up-regulated in all strains. The latter gene is predicted to facilitate the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate, which may also in turn explain the...”
- Transcriptional response of Enterococcus faecalis V583 to erythromycin
Aakra, Antimicrobial agents and chemotherapy 2005 - “...Cellular processes Cellular processes Cellular processes EF0105 EF0289 EF0290 EF0676 EF0891 EF0902 0 0 0.71 0 1.00 0.75 0 0 0 0 1.08 0.73 1.37 0 0 0 0.96...”
LSEI_1486 Aspartate/tyrosine/aromatic aminotransferase from Lactobacillus casei ATCC 334
33% identity, 90% coverage
LP_RS11350 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Lactiplantibacillus plantarum WCFS1
F9URG9 Aminotransferase from Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
lp_2684 aromatic amino acid specific aminotransferase from Lactobacillus plantarum WCFS1
31% identity, 93% coverage
FTL_0789 aspartate aminotransferase from Francisella tularensis subsp. holarctica
31% identity, 89% coverage
- Systems approach to investigating host-pathogen interactions in infections with the biothreat agent Francisella. Constraints-based model of Francisella tularensis
Raghunathan, BMC systems biology 2010 - “...+ FTL_1262 FTT_0945 + + FTL_0838 FTT_1124 metN + + FTL_0837 FTT_1125 metIQ + + FTL_0789 FTT_1165c aspC2 - + FTL_0606 FTT_1450 wbtM - + FTL_0594 FTT_1462c wbtC - + FTL_0592 FTT_1464c wbtA - + FTL_0304 FTT_1490 - + FTL_1701 FTT_1631c gplX - + FTL_0058 FTT_1168...”
- Exploitation of host cell biology and evasion of immunity by francisella tularensis
Asare, Frontiers in microbiology 2010 - “...4,6-dehydratase, WbtM, O-antigen polysaccharide biosynthesis FTL_0766 ggt Gamma-glutamyl transpeptidase; amino acid, arachidonic acid, and glutathione FTL_0789 aspC2 Aspartate aminotransferase; amino acid biosynthesis FTL_1262 Chorismate family binding protein; aromatic amino acid, and folate biosynthesis FTL_1415 capC Capsule biosynthesis protein CapC FTL_1416 capB Capsule biosynthesis protein CapB FTN_0020...”
- Working toward the future: insights into Francisella tularensis pathogenesis and vaccine development
Pechous, Microbiology and molecular biology reviews : MMBR 2009 - “...from http://mmbr.asm.org/ on April 16, 2019 by guest FTL_0789 Function VOL. 73, 2009 VACCINES FOR FRANCISELLA 699 TABLE 3--Continued Locus tag Name katG FTT0708...”
- Identification of Francisella tularensis Himar1-based transposon mutants defective for replication in macrophages
Maier, Infection and immunity 2007 - “...1 3 1 2 2 1 1 FTL_0838 FTL_0837 FTL_0789 FTL_0766 FTT1124 FTT1125 FTT1165c FTT1181c 5 9 3 7 FTL_0706 FTL_0606 FTL_0594 FTL_0592 FTL_0304 FTL_0544 FTL_1701...”
- Genome-wide identification of Francisella tularensis virulence determinants
Su, Infection and immunity 2007 - “...FTL_0382 FTL_0387 FTL_0483 FTL_0485 FTL_0525 FTL_0584 FTL_0789 FTL_0846 FTL_0960 FTL_1240 FTL_1266 FTL_1273 FTL_1274 FTL_1275 FTL_1419 FTL_1553 FTL_1554...”
O66630 LL-diaminopimelate aminotransferase (EC 2.6.1.83) from Aquifex aeolicus (see paper)
31% identity, 92% coverage
5wmlA / Q9SIE1 Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
31% identity, 88% coverage
- Ligands: 4'-deoxy-4'-aminopyridoxal-5'-phosphate; glutamic acid (5wmlA)
sll0006 aspartate aminotransferase from Synechocystis sp. PCC 6803
33% identity, 91% coverage
AAPAT_NITEU / Q82WA8 Aspartate/prephenate aminotransferase; AspAT / PAT; EC 2.6.1.1; EC 2.6.1.79 from Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298) (see paper)
31% identity, 89% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:24302739). Can also transaminate prephenate in the presence of glutamate, with lower efficiency (PubMed:24302739).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + 2-oxoglutarate = prephenate + L-glutamate (RHEA:22880)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms
Graindorge, The Journal of biological chemistry 2014 - “...Q55128, Q55828, Q3AJX2, Q82IK5, Q82DR2, Q82JN7, Q82EU6, O50434, Q82WA8, Q82UJ8, Q82S89, and Q82TJ4. 1 Supported by a Ph.D. fellowship from the University of...”
LHK_01340 aspartate aminotransferase from Laribacter hongkongensis HLHK9
31% identity, 89% coverage
- General metabolism of Laribacter hongkongensis: a genome-wide analysis
Curreem, Cell & bioscience 2011 - “...LHK_02172 Fumarate, oxaloacetate, ammonia, ATP TCA cycle Argininosuccinate lyase LHK_03122 L-aspartate oxidase LHK_00001 Aspartate aminotransferase LHK_01340 Glutamine Glutamine synthetase LHK_01876 -ketoglutarate, ammonia TCA cycle Glutamate dehydrogenase LHK_01886 Glycine Glycine cleavage system P-protein LHK_02722 Ammonia, CO 2 , NADH Ammonia assimilation Glycine cleavage system H protein LHK_02723...”
mfnC / Q58097 2-furaldehyde phosphate aminotransferase (EC 2.6.1.108) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (see 2 papers)
MFNC_METJA / Q58097 (5-formylfuran-3-yl)methyl phosphate transaminase; 4-HFC-P:alanine aminotransferase; EC 2.6.1.108 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 2 papers)
Q58097 (5-formylfuran-3-yl)methyl phosphate transaminase (EC 2.6.1.108) from Methanocaldococcus jannaschii (see paper)
MJ0684 aspartate aminotransferase (aspB2) from Methanocaldococcus jannaschii DSM 2661
30% identity, 90% coverage
- function: Catalyzes the transamination reaction between 4- (hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) and alanine to produce pyruvate and 5-(aminomethyl)-3-furanmethanol phosphate (F1- P), the precursor for the furan moiety in methanofuran.
catalytic activity: 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + L-alanine = [5-(aminomethyl)-3-furyl]methyl phosphate + pyruvate (RHEA:43564)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Biosynthesis of the 5-(Aminomethyl)-3-furanmethanol moiety of methanofuran
Miller, Biochemistry 2014 (PubMed)- “...purified pyridoxal 5-phosphate-dependent aminotransferase, derived from the MJ0684 gene, was found to be specific for catalyzing the transamination reaction...”
- “...by 4-HFC-P:alanine aminotransferase (MfnC), the product of the MJ0684 gene. Support for this pathway comes from the identification of 4-HFC-P and F1-P in M....”
- β-alanine biosynthesis in Methanocaldococcus jannaschii
Wang, Journal of bacteriology 2014 - “...enzyme was responsible. The products of the MJ0610, MJ0684, and MJ0959 genes are predicted to encode PLP-dependent enzymes for which no function has yet...”
lmo2252 similar to aspartate aminotransferase from Listeria monocytogenes EGD-e
31% identity, 89% coverage
- Transcriptomic response of Listeria monocytogenes to iron limitation and Fur mutation
Ledala, Applied and environmental microbiology 2010 - “...2.47 Amino acid biosynthesis lmo2090 lmo2091 lmo2252 Argininosuccinate synthase (argG) Argininosuccinate lyase (argH) Aspartate aminotransferase (aspB) Energy...”
- “...In our experiments, various other genes, lmo0569 (hisZ), lmo2252 (aspartate aminotransferase), and amino acid transport genes of the ABC type, lmo1738, lmo1739,...”
aspB-2 / P14909 aspartate aminotransferase subunit (EC 2.6.1.1) from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (see paper)
P14909 aspartate transaminase (EC 2.6.1.1) from Saccharolobus solfataricus (see 5 papers)
SSO0897 Aspartate aminotransferase (aspB-2) from Sulfolobus solfataricus P2
27% identity, 92% coverage
LOC109013250 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase-like from Juglans regia
31% identity, 73% coverage
AAT_STRAW / Q82DR2 Aspartate aminotransferase; AAT; AspAT; EC 2.6.1.1 from Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) (see paper)
34% identity, 88% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:24302739). Does not have prephenate aminotransferase activity (PubMed:24302739).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms
Graindorge, The Journal of biological chemistry 2014 - “...numbers: Q02635, A3PMF8, P54691, Q55128, Q55828, Q3AJX2, Q82IK5, Q82DR2, Q82JN7, Q82EU6, O50434, Q82WA8, Q82UJ8, Q82S89, and Q82TJ4. 1 Supported by a Ph.D....”
LOC108987596 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase-like from Juglans regia
31% identity, 75% coverage
- Combined transcriptomic and metabolomic analysis of the mechanism by which <i>Bacillus velezensis</i> induces resistance to anthracnose in walnut
Wang, Frontiers in microbiology 2024 - “...LOC108996646; LOC108985317; LOC108979963; LOC109004987; LOC108996947; LOC108988942; LOC108999180; LOC108988131; LOC108984074; LOC108981179; LOC108998652; LOC108992456; LOC108980812 LOC108989738; LOC109018267; LOC108987596; LOC108984851; LOC109019460; LOC108988131; LOC108984074; LOC108995469; LOC109009357; LOC108982978; LOC109011808; LOC108981208; LOC108983086; LOC109010098; LOC109002259; LOC109017388; LOC109003474; LOC109002260 DEM up D-xylulose 5-phosphate; 5-O-caffeoylquinic acid; Palmitic acid; UDP-N-acetyl--D-glucosamine; UDP-N-acetylglucosamine; -ketoglutaric acid; ferulic acid Spermine;...”
- “...LOC118347625; LOC108992695; LOC109019734; LOC118347949; LOC109009364; LOC109009682; LOC109002958; LOC108991725; LOC109011733; LOC108989481; LOC109005096; LOC118348746; LOC109004928; LOC109013250; LOC109018267; LOC108987596; LOC108996646; LOC109012843; LOC109012863; LOC109007996; LOC109007998; LOC108983187 DEM up / Coumarin; adenosine 5-diphosphate (ADP) Group B.v.: solely inoculated with B. velezensis ; Group CK: blank control; Group C.g.: solely inoculated with...”
PFLU3176 aspartate aminotransferase from Pseudomonas fluorescens SBW25
32% identity, 90% coverage
lpg0070 aspartate aminotransferase A from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
31% identity, 90% coverage
8wkjA / A0A130QXX8 The crystal structure of aspartate aminotransferases lpg0070 from legionella pneumophila (see paper)
31% identity, 90% coverage
- Ligand: pyridoxal-5'-phosphate (8wkjA)
SAG0019 aminotransferase, class I from Streptococcus agalactiae 2603V/R
33% identity, 89% coverage
DR75_1102 pyridoxal phosphate-dependent aminotransferase from Enterococcus faecalis ATCC 29212
31% identity, 88% coverage
ING2E5A_3029 pyridoxal phosphate-dependent aminotransferase from Petrimonas mucosa
31% identity, 89% coverage
BT_2415 aspartate aminotransferase from Bacteroides thetaiotaomicron VPI-5482
33% identity, 91% coverage
ORF01685 aminotransferase, classes I and II from Desulfovibrio vulgaris Hildenborough
33% identity, 90% coverage
EF2372 aspartate aminotransferase from Enterococcus faecalis V583
31% identity, 88% coverage
Q82EU6 N-succinyldiaminopimelate aminotransferase from Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680)
37% identity, 87% coverage
- Three different classes of aminotransferases evolved prephenate aminotransferase functionality in arogenate-competent microorganisms
Graindorge, The Journal of biological chemistry 2014 - “...A3PMF8, P54691, Q55128, Q55828, Q3AJX2, Q82IK5, Q82DR2, Q82JN7, Q82EU6, O50434, Q82WA8, Q82UJ8, Q82S89, and Q82TJ4. 1 Supported by a Ph.D. fellowship from the...”
lp_1280 aromatic amino acid specific aminotransferase from Lactobacillus plantarum WCFS1
30% identity, 89% coverage
AAPAT_CHLTE / Q8KDS8 Aspartate/prephenate aminotransferase; AspAT / PAT; EC 2.6.1.1; EC 2.6.1.78 from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (Chlorobium tepidum) (see paper)
31% identity, 88% coverage
- function: Catalyzes the reversible conversion of aspartate and 2- oxoglutarate to glutamate and oxaloacetate (PubMed:25070637). Can also transaminate prephenate in the presence of aspartate (PubMed:25070637).
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
catalytic activity: L-arogenate + oxaloacetate = prephenate + L-aspartate (RHEA:20445)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
LOC109018267 bifunctional aspartate aminotransferase and glutamate/aspartate-prephenate aminotransferase-like from Juglans regia
31% identity, 75% coverage
- Combined transcriptomic and metabolomic analysis of the mechanism by which <i>Bacillus velezensis</i> induces resistance to anthracnose in walnut
Wang, Frontiers in microbiology 2024 - “...LOC108998278; LOC108996646; LOC108985317; LOC108979963; LOC109004987; LOC108996947; LOC108988942; LOC108999180; LOC108988131; LOC108984074; LOC108981179; LOC108998652; LOC108992456; LOC108980812 LOC108989738; LOC109018267; LOC108987596; LOC108984851; LOC109019460; LOC108988131; LOC108984074; LOC108995469; LOC109009357; LOC108982978; LOC109011808; LOC108981208; LOC108983086; LOC109010098; LOC109002259; LOC109017388; LOC109003474; LOC109002260 DEM up D-xylulose 5-phosphate; 5-O-caffeoylquinic acid; Palmitic acid; UDP-N-acetyl--D-glucosamine; UDP-N-acetylglucosamine; -ketoglutaric acid; ferulic acid...”
- “...LOC108992092; LOC118347625; LOC108992695; LOC109019734; LOC118347949; LOC109009364; LOC109009682; LOC109002958; LOC108991725; LOC109011733; LOC108989481; LOC109005096; LOC118348746; LOC109004928; LOC109013250; LOC109018267; LOC108987596; LOC108996646; LOC109012843; LOC109012863; LOC109007996; LOC109007998; LOC108983187 DEM up / Coumarin; adenosine 5-diphosphate (ADP) Group B.v.: solely inoculated with B. velezensis ; Group CK: blank control; Group C.g.: solely inoculated...”
lmo1006 similar to aminotransferases (to B. subtilis PatA protein) from Listeria monocytogenes EGD-e
31% identity, 91% coverage
- Comparative genomics analysis to explore the biodiversity and mining novel target genes of Listeria monocytogenes strains from different regions
Zhang, Frontiers in microbiology 2024 - “...469 This study tilS lmo0219 1946 343 (100%) 0 tRNA(Ile)-lysidine synthase 648 This study dapX lmo1006 1,146 343 (100%) 0 Putative N-acetyl-LL-diaminopimelate aminotransferase 381 This study iolC lmo0385 978 343 (100%) 0 5-dehydro-2-deoxygluconokinase 325 This study gshAB lmo2770 2,331 343 (100%) 0 Glutathione biosynthesis bifunctional protein...”
- In vivo transcriptional profiling of Listeria monocytogenes and mutagenesis identify new virulence factors involved in infection
Camejo, PLoS pathogens 2009 - “...), and amino acids of the aspartate and glutamate families ( ansB , lmo0594 , lmo1006 , lmo1011 , lmo2413 and glnA , lmo2770 , respectively), was also increased in vivo ( Table S8 ). Significantly, mannose ( lmo0781lmo0784 ), maltose ( lmo0278 ) and cellobiose...”
- Presence of GadD1 glutamate decarboxylase in selected Listeria monocytogenes strains is associated with an ability to grow at low pH
Cotter, Applied and environmental microbiology 2005 - “...succinyl-coenzyme A or by the products of lmo1006 and lmo1005, putatively encoding an aminotransferase and a 3-hydroxyisobutyrate dehydrogenase, respectively,...”
APH_0660 aspartate aminotransferase from Anaplasma phagocytophilum HZ
33% identity, 90% coverage
H3R21_07190 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Lactobacillus sp. W8172
30% identity, 91% coverage
BVU_0144 aspartate aminotransferase from Bacteroides vulgatus ATCC 8482
30% identity, 89% coverage
SMc02251 PUTATIVE AMINOTRANSFERASE PROTEIN from Sinorhizobium meliloti 1021
33% identity, 89% coverage
- Transcriptomic Insight in the Control of Legume Root Secondary Infection by the Sinorhizobium meliloti Transcriptional Regulator Clr
Zou, Frontiers in microbiology 2017 - “...CheR chemotaxis protein methyltransferase -1,4759 -2,4545 smc03049 FlgL putative flagellar hook associated protein -1,08 -2,1779 smc02251 Putative aminotransferase protein -1,3324 -1,9797 smc04114 PilA1 putative pilin subunit protein -1,3701 -1,9591 smc01122 Conserved hypothetical protein -1,2814 -1,7944 smb20025 Conserved hypothetical protein -1,7088 -1,716 smc01468 CheW2 chemotaxis protein -1,717...”
- Transcriptome analysis of the role of GlnD/GlnBK in nitrogen stress adaptation by Sinorhizobium meliloti Rm1021
Yurgel, PloS one 2013 - “...glutamine synthetase type I (SMc01594), glutamine synthetase III ( glnT ) and an aspartate aminotransferase (SMc02251). The expression of several amino acid and oligopeptide transporters ( tauABC , oopABCD, SMc023572359, SMc02257-02258-02260, SMc0013800139, hypNMPQ ) was affected by mutations in the GlnD-GlnBK regulatory cascade. The transporters for...”
T303_01310 pyridoxal phosphate-dependent aminotransferase from Streptococcus thermophilus ASCC 1275
31% identity, 89% coverage
LOC100780254 aminotransferase superfamily protein from Glycine max
30% identity, 79% coverage
PA3798 putative aminotransferase from Pseudomonas aeruginosa PAO1
35% identity, 89% coverage
- Tailored Pyridoxal Probes Unravel Novel Cofactor-Dependent Targets and Antibiotic Hits in Critical Bacterial Pathogens
Pfanzelt, Angewandte Chemie (International ed. in English) 2022 - “...transamination.[ 39 , 40 ] F)UV/Vis screening from 300 to 600nm of aminotransferases PA3659 and PA3798 with 20 l amino acids (AA) and a mixture of 11 d amino acids (each 10 equivalents). Changes in absorbance at 390 or 430nm (external aldimine) were calculated and normalised....”
- “...(Figure 4 D). Furthermore, two putative aminotransferases (ATs) (by InterPro [29] and GO [42] ), PA3798 and PA3659 , identified in P. aeruginosa , were cloned and overexpressed for a closer inspection of substrate scope. PLP has different absorbance properties during its catalytic cycle in transamination...”
- A High-Throughput Method for Identifying Novel Genes That Influence Metabolic Pathways Reveals New Iron and Heme Regulation in Pseudomonas aeruginosa
Glanville, mSystems 2021 - “...a predicted kynurenine aminotransferase, whose precise function remains to be determined ( 84 ) ( PA3798 ; enriched by >41-fold) ( TableS1A ). In addition to PQS, several other metabolic pathways connected with that of chorismate were identified in the screen, including that of (i) the...”
- Tryptophan catabolism in Pseudomonas aeruginosa and potential for inter-kingdom relationship
Bortolotti, BMC microbiology 2016 - “...several hits. The best match originating from P. aeruginosa was from the open reading frame PA3798 ( ybdL ). We also found with lower BLAST scores GntR (PA2320) and PhhC (PA0870), an essential aminotransferase for aromatic amino acid catabolism. However, the difference between anthranilate and kynurenic...”
Q5N9Z8 Os01g0871300 protein from Oryza sativa subsp. japonica
A3A017 Aspartate aminotransferase from Oryza sativa subsp. japonica
30% identity, 79% coverage
Ot16g00690 Aminotransferases,class-I, pyridoxal-phosphate-binding site from Ostreococcus tauri
35% identity, 79% coverage
- Shikimate and phenylalanine biosynthesis in the green lineage
Tohge, Frontiers in plant science 2013 - “...Zm03g31000 Bd2g50800 Os01g55870 Osi01g52850 Sb04G005480 Zm05g21270 Bd3g06050 Os02g08410 Osi02g08160 Zm08g34320 Os12g38900 Zm08g34330 PAT Cr02g15900 Mrcc06g00860 Ot16g00690 Sb03G041180 Zm03g25600 Bd2g24300 Os01g65090 Osi01g61700 Sb09G021360 Zm08g15210 Bd2g56330 ADT Cr06g02760 Mrcc01g05870 Ot01g01250 Sb01G038740 Zm01g12020 Bd5g09020 Os04g33390 Osi03g16350 Sb06G015310 Zm02g16320 Bd5g09030 Os03g17730 Osi04g25440 Zm10g16000 Bd1g16517 Os07g49390 Osi07g41390 Bd1g65800 No. ID 13...”
D7SW04 Aminotransferase class I/classII large domain-containing protein from Vitis vinifera
31% identity, 76% coverage
LAR_0041 aromatic amino acid aminotransferase from Lactobacillus reuteri JCM 1112
Lreu_0044 aromatic amino acid aminotransferase from Lactobacillus reuteri DSM 20016
32% identity, 89% coverage
- Lactobacillus maintains healthy gut mucosa by producing L-Ornithine
Qi, Communications biology 2019 - “...of L. reuteri DSM 20016 (NCBI GI 148530277, Lreu_0044) and JCM 1112 (NCBI GI 183223999, LAR_0041) were used for identifying a homologous gene in the genome of L. reuteri ATCC PTA 4659. The locus of the gene encoding the OCT and flanking nucleotide sequences in L....”
- Lactobacillus maintains healthy gut mucosa by producing L-Ornithine
Qi, Communications biology 2019 - “...OCT-deficient lactobacillus Ornithine carbamoyltransferase (OCT) nucleotide sequences of L. reuteri DSM 20016 (NCBI GI 148530277, Lreu_0044) and JCM 1112 (NCBI GI 183223999, LAR_0041) were used for identifying a homologous gene in the genome of L. reuteri ATCC PTA 4659. The locus of the gene encoding the...”
Q8BTY1 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Mus musculus (see paper)
35% identity, 72% coverage
D3D012 Aminotransferase class I and II from Parafrankia sp. EUN1f
37% identity, 86% coverage
Q8YTF2 aspartate transaminase (EC 2.6.1.1) from Nostoc sp. (see paper)
alr2765 aminotransferase from Nostoc sp. PCC 7120
32% identity, 89% coverage
Q9RAT0 aromatic-amino-acid transaminase (EC 2.6.1.57) from Lactococcus lactis (see paper)
llmg_0066 aromatic amino acid aminotransferase from Lactococcus lactis subsp. cremoris MG1363
32% identity, 89% coverage
Sb03g041180 No description from Sorghum bicolor
31% identity, 81% coverage
- Shikimate and phenylalanine biosynthesis in the green lineage
Tohge, Frontiers in plant science 2013 - “...Bd2g50800 Os01g55870 Osi01g52850 Sb04G005480 Zm05g21270 Bd3g06050 Os02g08410 Osi02g08160 Zm08g34320 Os12g38900 Zm08g34330 PAT Cr02g15900 Mrcc06g00860 Ot16g00690 Sb03G041180 Zm03g25600 Bd2g24300 Os01g65090 Osi01g61700 Sb09G021360 Zm08g15210 Bd2g56330 ADT Cr06g02760 Mrcc01g05870 Ot01g01250 Sb01G038740 Zm01g12020 Bd5g09020 Os04g33390 Osi03g16350 Sb06G015310 Zm02g16320 Bd5g09030 Os03g17730 Osi04g25440 Zm10g16000 Bd1g16517 Os07g49390 Osi07g41390 Bd1g65800 No. ID 13 VV...”
WD1029 aspartate aminotransferase from Wolbachia endosymbiont of Drosophila melanogaster
29% identity, 91% coverage
SCO3658 hypothetical protein from Streptomyces coelicolor A3(2)
38% identity, 81% coverage
- A BioBricks toolbox for metabolic engineering of the tetracenomycin pathway
Nguyen, Biotechnology journal 2022 - “...for single-copy chromosomal engineering ( Supplementary Figure 1 ). The TG1 integrase recombines into the sco3658 aminotransferase gene in the middle of the chromosome [ 25 ] . In addition, we obtained the BioBrick-compatible vector pOSV808, which includes the VWB actinophage integrase, attP site, oriT ,...”
D9S304 LL-diaminopimelate aminotransferase from Thermosediminibacter oceani (strain ATCC BAA-1034 / DSM 16646 / JW/IW-1228P)
30% identity, 90% coverage
- Allelic imbalance in the miR-31 host gene locus in lung cancer--its potential role in carcinogenesis.
Okudela, PloS one 2014 - “...CDKN2A gene locus (D9S974) and another distant locus of the short arm of chromosome 9 (D9S304) were also analyzed using the primer sets of F 5- GAGCCTGGTCTGGATCATAA and R 5- AAGCTTACAGAACCAGACAG , and F 5- GTGCACCTCTACACCCAGAC and R 5- TGTGCCCACACACATCTATC , respectively. In situ hybridization for...”
- “...cell lines The statuses of the miR-31 gene locus, CDKN2A locus, and the adjacent locus (D9S304), were investigated using PCR analysis. Among the forty-one cell lines examined, six cell lines (14.6%, 6/41 cell lines; H322, TKB1, TKB2, TKB5, TKB8, and TKB9) lost the miR-31 host gene...”
- Selection of microsatellite markers for bladder cancer diagnosis without the need for corresponding blood.
van, PloS one 2012 - “...152176 9q 0.75 F CAAATTTGGCCTTGAACCAT R AGCCCAGATATCCCCAAGTT D9S299 178198 9q 0.70 F AAGTGTTGCATCAGAGCCTC R AGTGTGAACATTATTTCATTCTGG D9S304 135175 9q 0.86 F GTGCACCTCTACACCCAGAC R TGTGCCCACACACATCTATC D9S752 178201 9q 0.73 F CAGAGGTTGCAGTGAGCTA R GCAAAGTCAGGCCATTATAC D9S1118 141177 9q 0.79 F CAGGATATTATGTGATGGAATCC R CTGCTGACTCCAAAAATATGC D11S1981 134178 11p 0.85 F AATTCCTTTACTCCAGAAAGG R...”
- “...1.04 0.08 0.851.19 D8S1130 1.15 0.09 1.001.40 D9S252 1.08 0.06 0.971.23 D9S299 1.10 0.07 0.951.27 D9S304 1.10 0.11 0.861.33 D9S752 1.12 0.08 0.921.31 D9S1118 1.10 0.10 0.971.42 D11S1981 1.14 0.09 0.931.39 D11S1999 1.12 0.09 0.931.45 D17S969 1.11 0.08 0.951.33 G10693 1.11 0.07 1.001.29 Performance of the...”
- [Allele-specific chromosome 9p deletion in oral cancer].
Xiao, Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology 2001 (PubMed) - Post-mortem forensic identity testing: application of PCR to the identification of fire victim.
Soares-Vieira, Sao Paulo medical journal = Revista paulista de medicina 2000 - “...cardiac chamber, and 15 different loci (D1S80, ApoB, D17S30, D3S1744, D18S849, D12S1090, FGA, D7S820, D1S533, D9S304, HUMCSF1PO, HUMTPOX, HUMTHO1, amelogenin and HLA-DQA1) were analyzed using the PCR technique. Results from all loci typing of the corpse were then compared to that of his alleged biological parents,...”
- “...de cmara cardaca e 15 loci (D1S80, ApoB, D17S30, D3S1744, D18S849, D12S1090, FGA, D7S820, D1S533, D9S304, HUMCSF1PO, HUMTPOX, HUMTHO1, amelogenina e HLA-DQA1) foram analisados utilizando-se a tcnica da PCR. Os resultados de todos os loci analisados a partir de DNA genmico extrado do corpo carbonizado foram...”
cg0931 aminotransferase from Corynebacterium glutamicum ATCC 13032
34% identity, 88% coverage
- Screening of a genome-reduced Corynebacterium glutamicum strain library for improved heterologous cutinase secretion
Hemmerich, Microbial biotechnology 2020 - “...CR099 MB001 IS Cg 12 Baumgart et al . ( 2013 ) GRS21 CR099 rrnB cg0931 Unthan et al . ( 2015b ) GRS25 CR099 cg1281cg1289 Unthan et al . ( 2015b ) GRS41 CR099 cg2801cg2828 Unthan et al . ( 2015b ) GRS45 CR099 cg2990cg3006...”
- “...) GRS55 CR099 cg3000cg3006 This study GRS56 CR099 rrnB This study GRS21_41 CR099 cg2801cg2828 rrnB cg0931 Unthan et al . ( 2015b ) GRS41_51 CR099 cg2801cg2828 rrnC cg3298 Unthan et al . ( 2015b ) GRS48_52 CR099 cg3102cg3111 cg3263cg3301 Baumgart et al . ( 2018 )...”
CD0107 aspartate aminotransferase from Clostridium difficile 630
27% identity, 90% coverage
SACOL1058 aminotransferase, class I from Staphylococcus aureus subsp. aureus COL
SAUSA300_0952 aminotransferase, class I from Staphylococcus aureus subsp. aureus USA300_FPR3757
28% identity, 91% coverage
C3K1K9 Aminotransferase from Pseudomonas fluorescens (strain SBW25)
33% identity, 89% coverage
NJ7G_1211 pyridoxal phosphate-dependent aminotransferase from Natrinema sp. J7-2
33% identity, 90% coverage
- Effects of salinity on the cellular physiological responses of Natrinema sp. J7-2
Mei, PloS one 2017 - “...Upregulated genes Ala, Asp and Glu nat: NJ7G_0685, 1314, 1637, 2607, 3072, 3447, 3448 nat: NJ7G_1211, 1599, 1939, 1940, 1943, 2460, 2673, Phe nat: NJ7G_ 1211, 1899, 1908,1914, 1915,1916, 3496 beta-Ala nat: NJ7G_0204, 1012 nat: NJ7G_ 1141, 1940, 1943, 4348, Porphyrin and chlorophyll nat: NJ7G_ 2498...”
Q58FK9 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Rattus norvegicus (see paper)
31% identity, 73% coverage
- Fluoxetine Enhances Synaptic Vesicle Trafficking and Energy Metabolism in the Hippocampus of Socially Isolated Rats.
Filipović, International journal of molecular sciences 2022 - “...shock 70kDa protein 12A (Predicted), isoform CRA_a D3ZC55 Hspa12a 1.86 3 3 Kynurenine--oxoglutarate transaminase 3 Q58FK9 Kyat3 1.86 3 3 Adducin 1 (Alpha), isoform CRA_b A0A0G2JSM7 Add1 1.86 5 4 Synaptotagmin-1 P21707 Syt1 1.85 4 3 Dihydropyrimidinase-related protein Q9JMG8 N/A 1.83 3 3 Glutaminase kidney isoform,...”
- Proteomic Expression Changes in Large Cerebral Arteries After Experimental Subarachnoid Hemorrhage in Rat Are Regulated by the MEK-ERK1/2 Pathway.
Müller, Journal of molecular neuroscience : MN 2017 - “...mitochondrial 0.87 P19804 Nucleoside diphosphate kinase B 0.86 P85972 Vinculin 0.85 P15865 Histone H1.2 0.84 Q58FK9 Kynurenine--oxoglutarate transaminase 3 0.84 P62260 14-3-3 protein epsilon 0.84 Q5XI78 2-Oxoglutarate dehydrogenase, mitochondrial 0.82 B2GV06 Succinyl-CoA:3-ketoacid-coenzyme A transferase 1, mitochondrial 0.82 P31977 Ezrin 0.81 Q01129 Decorin 0.80 O88483 [Pyruvate dehydrogenase...”
- Functional proteomic analysis of corticosteroid pharmacodynamics in rat liver: Relationship to hepatic stress, signaling, energy regulation, and drug metabolism.
Ayyar, Journal of proteomics 2017 - “...Q07523 Hao2 Hydroxyacid oxidase 2 Oxidation of L-alpha-hydroxy acids as well as L-alpha-amino acids DOWN Q58FK9 Kat3 Kynurenine-oxoglutarate transaminase 3 L-kynurenine to kynurenic acid UP Q5XIT9 Mccc2 Methylcrotonoyl-CoA carboxylase beta chain Amino acid (L-leucine) metabolism UP P04182 Oat Ornithine aminotransferase, mitochondrial Formation of proline from acid...”
SA0902 hypothetical protein from Staphylococcus aureus subsp. aureus N315
28% identity, 91% coverage
Mhun_2943 LL-diaminopimelate aminotransferase from Methanospirillum hungatei JF-1
YP_504354 aminotransferase, class I and II from Methanospirillum hungatei JF-1
32% identity, 92% coverage
- Synonymous codon bias and functional constraint on GC3-related DNA backbone dynamics in the prokaryotic nucleoid
Babbitt, Nucleic acids research 2014 - “...villosus KIN24-T80 MetviDRAFT_0533 Methanocella paludicola SANAE MCPlv_0872 Methanomethylovorans hollandica DSM 15978 Metho_1006 Methanospirillum hungatei JF-1 Mhun_2943 Methanobacterium thermoautotrophicum str. Delta H* Mth_52 Ferroglobus placidus DSM 10642 Ferp_0809 Bacteria Bacteroides fragilis BF2643 Chlamydia trachomatis D(s)2923 CtraD_010100002050 Clostridium thermocellum DSM 2360 ClothDRAFT_2722 Ethanoligenens harbinense YUAN-3 Ethha_0920 Faecalibacterium prausnitzii...”
- Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase
Nachar, Frontiers in microbiology 2012 - “...PCC 7120 (NP_488367), Gloeobacter violaceus PCC 7421 (NP_927054), Methanosaeta thermophila PT (YP_843230), Methanospirillum hungatei JF1 (YP_504354), Dehalococcoides CBDB1 (YP_307791), Methanococcoides burtonii DSM 6242 (YP_565702), Methanosarcina barkeri Fusaro (YP_306095), Methanosarcina acetivorans C2A (NP_616639), Syntrophobacter fumaroxidans MPOB (YP_844192), Planctomyces brasiliensis DSM 5305 (YP_004269630), Planctomyces limnophilus DSM 3776 (YP_003632005),...”
ZMO1727 aminotransferase from Zymomonas mobilis subsp. mobilis ZM4
31% identity, 87% coverage
B1B6U5 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Haemaphysalis longicornis (see paper)
GI|170172430 kynurenine aminotransferase; EC 2.6.1.7 from Haemaphysalis longicornis (see paper)
32% identity, 79% coverage
PAT_PINPS / Q5F4K8 Aspartate aminotransferase; PpAAT; EC 2.6.1.1 from Pinus pinaster (Maritime pine) (see paper)
Q5F4K8 aspartate transaminase (EC 2.6.1.1) from Pinus pinaster (see paper)
30% identity, 73% coverage
- function: Prokaryotic-type aspartate aminotransferase. Specific for aspartate and no activity with glutamine, asparagine, alanine, histidine, leucine, methionine, lysine, arginine, tryptophan, tyrosine, phenylalanine or kynurenine.
catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate (RHEA:21824)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer
APA386B_991 LL-diaminopimelate aminotransferase from Acetobacter pasteurianus 386B
32% identity, 88% coverage
YP_307791 putative aspartate aminotransferase from Dehalococcoides sp. CBDB1
31% identity, 90% coverage
- Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase
Nachar, Frontiers in microbiology 2012 - “...Gloeobacter violaceus PCC 7421 (NP_927054), Methanosaeta thermophila PT (YP_843230), Methanospirillum hungatei JF1 (YP_504354), Dehalococcoides CBDB1 (YP_307791), Methanococcoides burtonii DSM 6242 (YP_565702), Methanosarcina barkeri Fusaro (YP_306095), Methanosarcina acetivorans C2A (NP_616639), Syntrophobacter fumaroxidans MPOB (YP_844192), Planctomyces brasiliensis DSM 5305 (YP_004269630), Planctomyces limnophilus DSM 3776 (YP_003632005), Verrucomicrobiae bacterium DG1235...”
aat / Q9P9M8 alanine aminotransferase subunit (EC 2.6.1.2) from Pyrococcus furiosus (see paper)
Q9P9M8 alanine transaminase (EC 2.6.1.2) from Pyrococcus furiosus (see 3 papers)
PF1497 pyridoxal phosphate-dependent aminotransferase from Pyrococcus furiosus DSM 3638
NP_579226 putative transaminase from Pyrococcus furiosus DSM 3638
29% identity, 91% coverage
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...( Chon et al., 2005 ; Okada et al., 2014 ). Concerning other groups, the PF1497 protein from P. furiosus (G3), referred to as AlaAT, displays highest activity with Ala. The enzyme did not display activity toward Phe and Tyr ( Ward et al., 2000 )....”
- “...G1 Aromatic aminotransferase Phe, Tyr, Trp 2-OG Ward et al. (2002) PF0522 G2 (Aspartate aminotransferase) PF1497 G3 Alanine aminotransferase Ala, Glu, (Asp, Ile, Leu) 2-OG, Pyr Ward et al. (2000) PF1702 G4 Aspartate aminotransferase Asp, (Glu) 2-OG, (Pyr, phenylpyruvate) Ward et al. (2002) PF0121 G6 Aromatic...”
- Impact of substrate glycoside linkage and elemental sulfur on bioenergetics of and hydrogen production by the hyperthermophilic archaeon Pyrococcus furiosus
Chou, Applied and environmental microbiology 2007 - “...Putative carbohydrate binding protein Aminotransferase PF0121 PF1497 3.1 3.4 2.6 2.2 Aromatic amino acid aminotransferase Alanine aminotransferase Oligopeptide...”
- Whole-genome DNA microarray analysis of a hyperthermophile and an archaeon: Pyrococcus furiosus grown on carbohydrates or peptides
Schut, Journal of bacteriology 2003 - “...that have been purified, PF0121 (4.5-fold) (3) and PF1497 (2.5-fold) (62). These transamination reactions produce various 2-ketoacids as well as glutamate from...”
- Alanine aminotransferase variants conferring diverse NUE phenotypes in Arabidopsis thaliana
McAllister, PloS one 2015 - “...Rong ze Yang, University of Maryland. Pyrococcus furiosus AlaAT ( PfAlaAT ) (GenBank accession no. NP_579226) was amplified from ATCC gDNA (DSM 3638) [ 35 ]. Restriction enzyme cut sites for Asc 1 and Pac 1 were added to the 5 and 3 ends, respectively, of...”
- Analysis of the enzymatic properties of a broad family of alanine aminotransferases
McAllister, PloS one 2013 - “...MtAlaAT2 contained the point mutation F177S. Pyrococcus furiosus AlaAT ( PfAlaAT ) (GenBank accession no. NP_579226) was amplified from ATCC gDNA (DSM 3638). Arabidopsis thaliana AlaAT1 ( AtAlaAT1 ) (TAIR reference no. AtG17290) and AlaAT2 ( AtAlaAT2 ) (TAIR reference no. At1G72330) sequences were obtained from...”
PP0858 aminotransferase, class I from Pseudomonas putida KT2440
33% identity, 90% coverage
lpg1459 aspartate aminotransferase from Legionella pneumophila subsp. pneumophila str. Philadelphia 1
32% identity, 89% coverage
LJ1390 aspartate aminotransferase from Lactobacillus johnsonii NCC 533
30% identity, 89% coverage
- L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches
van, Applied and environmental microbiology 2004 - “...to L. johnsonii proteins; however, two proteins, LJ0915 and LJ1390, were found to exhibit 23 and 25% amino acid identity, respectively, with AspC of Lactococcus...”
- “...the two previously identified PLP-dependent ATs, LJ0915 and LJ1390 (Table 2). LJ0915 showed 50% sequence identity to a recently described L. delbrueckii...”
- The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533
Pridmore, Proceedings of the National Academy of Sciences of the United States of America 2004 - “...oxaloacetate and Glu, using aspartate aminotransferase (aspC, LJ1390). Additionally, the presence of glutamine synthetase (glnA, LJ1614) and a probable...”
1xi9C / Q9P9M8 Alanine aminotransferase from pyrococcus furiosus pfu-1397077-001
29% identity, 92% coverage
- Ligand: pyridoxal-5'-phosphate (1xi9C)
KAT3_MOUSE / Q71RI9 Kynurenine--oxoglutarate transaminase 3; Cysteine-S-conjugate beta-lyase 2; Kynurenine aminotransferase 3; Kynurenine aminotransferase III; KATIII; Kynurenine--glyoxylate transaminase; Kynurenine--oxoglutarate transaminase III; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.63 from Mus musculus (Mouse) (see 2 papers)
Q71RI9 glutamine-pyruvate transaminase (EC 2.6.1.15); kynurenine-oxoglutarate transaminase (EC 2.6.1.7); cysteine-S-conjugate beta-lyase (EC 4.4.1.13) from Mus musculus (see 5 papers)
32% identity, 71% coverage
- function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others (PubMed:19029248). May catalyze the beta-elimination of S- conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (PubMed:19029248). Has transaminase activity towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine and asparagine with glyoxylate as an amino group acceptor (in vitro). Has lower activity with 2-oxoglutarate as amino group acceptor (in vitro) (PubMed:19029248).
catalytic activity: L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O (RHEA:65560)
catalytic activity: L-kynurenine + glyoxylate = kynurenate + glycine + H2O (RHEA:65896)
catalytic activity: 3-hydroxy-L-kynurenine + glyoxylate = xanthurenate + glycine + H2O (RHEA:65900)
catalytic activity: an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+) (RHEA:18121)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Age-related disruption of the proteome and acetylome in mouse hearts is associated with loss of function and attenuated by elamipretide (SS-31) and nicotinamide mononucleotide (NMN) treatment.
Whitson, GeroScience 2022 - Characterization and Proteomic-Transcriptomic Investigation of Monocarboxylate Transporter 6 Knockout Mice: Evidence of a Potential Role in Glucose and Lipid Metabolism
Jones, Molecular pharmacology 2019 (secret) - Mitochondrial Respiration Is Impaired during Late-Stage Hamster Prion Infection.
Faris, Journal of virology 2017
XP_006501494 kynurenine--oxoglutarate transaminase 3 isoform X1 from Mus musculus
32% identity, 77% coverage
H375_5400 pyridoxal phosphate-dependent aminotransferase from Rickettsia prowazekii str. Breinl
28% identity, 90% coverage
CCBL2 / Q6YP21 Kynurenine--oxoglutarate transaminase 3 (EC 2.6.1.7; EC 4.4.1.13) from Homo sapiens (see 3 papers)
Q6YP21 kynurenine-oxoglutarate transaminase (EC 2.6.1.7); cysteine-S-conjugate beta-lyase (EC 4.4.1.13) from Homo sapiens (see 3 papers)
30% identity, 71% coverage
- The Biology and Biochemistry of Kynurenic Acid, a Potential Nutraceutical with Multiple Biological Effects.
Alves, International journal of molecular sciences 2024 - “...207 ], and mechanisms are far from clear cut. 4.6. KAT III KAT III (Uniprot Q6YP21), most closely related in sequence to KAT I (unpublished alignment search using the tools provided in [ 156 ]), has 454 amino acids and, like KAT I, a preference for...”
- GSK3 inhibition reduces ECM production and prevents age-related macular degeneration-like pathology.
DiCesare, bioRxiv : the preprint server for biology 2023 - “...GRB2 Growth factor receptor-bound protein 2 0.502 P05386 RPLP1 60S acidic ribosomal protein P1 0.519 Q6YP21 KYAT3 Kynurenine--oxoglutarate transaminase 3 0.524 Q9UNH7 SNX6 Sorting nexin-6 0.525 P78344 EIF4G2 Eukaryotic translation initiation factor 4 gamma 2 0.526 Q16769 QPCT Glutaminyl-peptide cyclotransferase 0.527 O43405 COCH Cochlin 0.529 P26583...”
- Proteomic profile of follicular fluid from patients with polycystic ovary syndrome (PCOS) submitted to in vitro fertilization (IVF) compared to oocyte donors
Domingues, JBRA assisted reproduction 2019 - “...protein(PC00219) nucleic acid binding(GO:0005488) NA 1 Q63HN8 E3 ubiquitin-protein ligase RNF213 NA NA NA 1 Q6YP21 Kynurenine--oxoglutarate transaminase 3 transaminase(PC00220) transaminase activity(GO:0003824) NA 1 Q70CQ2 Ubiquitin carboxyl-terminal hydrolase 34 NA NA NA 1 Q7Z6M1 Rab9 effector protein with kelch motifs NA NA NA 1 Q86YQ8 Copine-8...”
- Proteomic Study to Survey the CIGB-552 Antitumor Effect
Rodríguez-Ulloa, BioMed research international 2015 - “...GNPAT 46.58 4.5 3.1 1.9 Q86Y56 HEAT repeat-containing protein 2 HEATR2 63.07 1.5 5 1.4 Q6YP21 Kynurenine-oxoglutarate transaminase 3 CCBL2 39.9 1.7 OFF 1.5 P43243 Matrin-3 MATR3 269.16 1 1.2 4.5 O75439 Mitochondrial-processing peptidase subunit beta PMPCB 99.73 2.9 1.2 1.1 O43847 Nardilysin NRD1 41.16 1.3...”
3e2yA / Q71RI9 Crystal structure of mouse kynurenine aminotransferase iii in complex with glutamine (see paper)
32% identity, 79% coverage
- Ligands: glutamine; 4'-deoxy-4'-aminopyridoxal-5'-phosphate (3e2yA)
NP_001008662 kynurenine--oxoglutarate transaminase 3 isoform 2 from Homo sapiens
30% identity, 76% coverage
- Cysteine conjugate beta-lyase 2 (CCBL2) expression as a prognostic marker of survival in breast cancer patients.
Meng, PloS one 2022 - GeneRIF: Cysteine conjugate beta-lyase 2 (CCBL2) expression as a prognostic marker of survival in breast cancer patients.
- The presence of kynurenine aminotransferases in the human cornea: Evidence from bioinformatics analysis of gene expression and immunohistochemical staining.
Matysik-Woźniak, Molecular vision 2017 - GeneRIF: Immunohistochemical analysis revealed the presence of KAT I, II, and III in all examined corneal sections.
- Kynurenine aminotransferase III and glutamine transaminase L are identical enzymes that have cysteine S-conjugate β-lyase activity and can transaminate L-selenomethionine.
Pinto, The Journal of biological chemistry 2014 - GeneRIF: human KAT III/CCBL2 possesses cysteine S-conjugate beta-lyase activity, as does mouse KAT II
- The kynurenine pathway in major depression: haplotype analysis of three related functional candidate genes.
Claes, Psychiatry research 2011 (PubMed)- GeneRIF: The haplotype of KAT III gene CGCTCT may have effect on the function of this enzyme in formation of kynurenic acid in some patients with major depressive episodes.
- Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson, PloS one 2010 - GeneRIF: Observational study of gene-disease association. (HuGE Navigator)
- Prefrontal cortex shotgun proteome analysis reveals altered calcium homeostasis and immune system imbalance in schizophrenia.
Martins-de-Souza, European archives of psychiatry and clinical neuroscience 2009 (PubMed)- GeneRIF: Using shotgun mass spectrometry, it was found that this protein is differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia.
- Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
Rose, Molecular medicine (Cambridge, Mass.) - GeneRIF: Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
BAD06364.1 aminotransferase from Thermus thermophilus (see paper)
39% identity, 72% coverage
1v2eA / Q75WK2 Crystal structure of t.Th hb8 glutamine aminotransferase complex with a-keto-g-methylthiobutyrate (see paper)
39% identity, 72% coverage
- Ligands: pyridoxal-5'-phosphate; 4-(methylsulfanyl)-2-oxobutanoic acid (1v2eA)
6l1lB / P39643 Apo-bacf structure from bacillus subtillis (see paper)
30% identity, 92% coverage
- Ligand: pyridoxal-5'-phosphate (6l1lB)
bacF / P39643 3-[(2S,5R)-5-hydroxy-7-oxabicyclo[4.1.0]heptan-2-yl]-2-oxopropanoate aminotransferase (EC 2.6.1.57) from Bacillus subtilis (strain 168) (see 2 papers)
BACF_BACSU / P39643 Transaminase BacF; Transaminase A; EC 2.6.1.- from Bacillus subtilis (strain 168) (see 3 papers)
NP_391649 phenylalanine aminotransferase forming tetrahydrotyrosine in bacilysin synthesis from Bacillus subtilis subsp. subtilis str. 168
BSU37690 transaminase from Bacillus subtilis subsp. subtilis str. 168
30% identity, 90% coverage
- function: Part of the bacABCDEF operon responsible for the biosynthesis of the nonribosomally synthesized dipeptide antibiotic bacilysin, composed of L-alanine and L-anticapsin. Bacilysin is an irreversible inactivator of the glutaminase domain of glucosamine synthetase (PubMed:20052993). Catalyzes the reductive amination of the C2 ketone of tetrahydro-hydroxyphenylpyruvate (H4HPP), with L-Phe as an amino donor, to yield tetrahydrotyrosine (H4Tyr) diastereomer (PubMed:22765234). D-Phe is not an effective amino donor (PubMed:22765234). BacF associated to BacG converts 3E,7R- and 3Z,7R- ex-H2HPP to 2S,4R,7R- and 2S,4S,7R-H4Tyr, respectively. Given that bacilysin has the 2S,4S stereochemistry in its anticapsin moiety, it is likely that the 2S,4S-H4Tyr is the diastereomer used for the biosynthesis (PubMed:22765234).
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Detection of Antagonistic Compounds Synthesized by Bacillus velezensis against Xanthomonas citri subsp. citri by Metabolome and RNA Sequencing
Rabbee, Microorganisms 2023 - “...isomerase bacC Q8KWT4 Dihydroanticapsin dehydrogenase bacD Q8KWT3 L-alanine-anticapsin ligase bacE Q8KWT2 Putative bacilysin exporter ywfG P39643 Probable aspartate aminotransferase ywfH P39644 Bacilysin biosynthesis oxidoreductase Plipastatin or Fengycin ppsA P39845 Plipastatin synthase subunit A ppsB P39846 Plipastatin synthase subunit B ppsC P39847 Plipastatin synthase subunit C ppsD...”
- Investigation of anticapsin biosynthesis reveals a four-enzyme pathway to tetrahydrotyrosine in Bacillus subtilis.
Mahlstedt, Biochemistry 2010 - GeneRIF: BacA, BacB, YwfH, and YwfG act in sequence in a four-enzyme pathway to make tetrahydrotyrosine, which has not previously been identified in B. subtilis.
- A Survey of Pyridoxal 5'-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis
Richts, Frontiers in molecular biosciences 2019 - “...subfamily Gardan et al., 1995 ; Molle et al., 2003a , b Antibacterial compounds BacF BSU37690 No 2.6.1.1 201B ( S. aureus , 40%) Aminotransferase Bacilysin biosynthesis AbrB (), CodY (), ScoC () MtnE (50%)/ PLP AAT class I family Inaoka et al., 2003 , 2009...”
- Data on genome analysis of Bacillus velezensis LS69
Liu, Data in brief 2017 - “...(locus_tag of the bacABCDE,ywfG : BSU37740 / BSU37730 / BSU37720 / BSU37710 / BSU37700 , BSU37690 ); macrolactin of B.amyloliquefaciens FZB42 (AJ 6340601.2); bacillaene of B.amyloliquefaciens FZB42 (AJ 634060.2); difficidin of B.amyloliquefaciens FZB42 (AJ 6340602.2); amylolysin of Bacillus amyloliquefaciens GA1 ( KC415250.1 ); amylocyclicin of B.amyloliquefaciens...”
- Secondary structural entropy in RNA switch (Riboswitch) identification
Manzourolajdad, BMC bioinformatics 2015 - “...3666640 3666796 reverse BSU35680 ggaB -490 0.4968 1335 mnaA BSU35660 0.908 7 3866327 3866483 reverse BSU37690 ywfG -1881 0.3503 79 eutD BSU37660 0.903 8 681153 681309 forward BSU06260 ydjN -201 0.3885 5731 yeaB BSU06320 0.899 9 2987548 2987704 reverse BSU29200 accA -104 0.4268 79 pfkA BSU29190...”
- “...3714883 3715039 forward BSU36030 ywrK -859 0.3949 2277 cotG BSU36070 0.922 3 3866327 3866483 reverse BSU37690 ywfG -1881 0.3503 79 eutD BSU37660 0.903 4 681153 681309 forward BSU06260 ydjN -201 0.3885 5731 yeaB BSU06320 0.899 5 1680274 1680430 forward BSU16080 ylqH 63 0.3822 79 sucC BSU16090...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...DapL paralogs of E. coli (b2379) and Bacillus subtilis (BSU37690) showed that these paralogs had lower Eds with archaeal DapL2 and methanococcal DapL groups and...”
- “...coli 2, b3359; Gloeobacter, glr4108; B. subtilis 1, BSU37690; B. subtilis 2, BSU13580; B. subtilis 3, BSU11220; B. subtilis 4, BSU03900; Bacteroides, BF2666;...”
HISP_16825 pyridoxal phosphate-dependent aminotransferase from Haloarcula hispanica N601
33% identity, 89% coverage
- Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica
Lee, MicrobiologyOpen 2020 - “...PROCESSES AND SIGNALING Signal transduction mechanisms Signal transduction histidine kinase 1.366 0.435 0.872 0.929 M HISP_16825 METABOLISM Amino acid transport and metabolism Aspartate/tyrosine/aromatic aminotransferase 1.069 0.725 0.472 1.322 M HISP_17040 METABOLISM Amino acid transport and metabolism phenylacetateCoA oxygenase 1.015 0.559 0.363 1.211 M HISP_17215 METABOLISM Inorganic...”
1u08A / P77806 Crystal structure and reactivity of ybdl from escherichia coli identify a methionine aminotransferase function. (see paper)
32% identity, 92% coverage
- Ligand: pyridoxal-5'-phosphate (1u08A)
YbdL / b0600 methionine transaminase (EC 2.6.1.88) from Escherichia coli K-12 substr. MG1655 (see 4 papers)
ybdL / P77806 methionine transaminase (EC 2.6.1.88) from Escherichia coli (strain K12) (see 3 papers)
YBDL_ECOLI / P77806 Methionine aminotransferase; Methionine-oxo-acid transaminase; EC 2.6.1.88 from Escherichia coli (strain K12) (see paper)
P77806 methionine transaminase (EC 2.6.1.88) from Escherichia coli (see paper)
ybdL / MB|P77806 aminotransferase ybdL; EC 2.6.1.- from Escherichia coli K12 (see 5 papers)
b0600 putative aminotransferase from Escherichia coli str. K-12 substr. MG1655
32% identity, 92% coverage
- function: Shows aminotransferase activity with methionine and histidine as substrates, and to a lesser extent also with phenylalanine.
catalytic activity: a 2-oxocarboxylate + L-methionine = 4-methylsulfanyl-2- oxobutanoate + an L-alpha-amino acid (RHEA:31763)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Gap-filling analysis of the iJO1366 Escherichia coli metabolic network reconstruction for discovery of metabolic functions
Orth, BMC systems biology 2012 - “...R07176 (R) IV global orphan R07463 (F) IV dadA (b1189) 2.00E-18 R07613 (R) II ydbL (b0600) 7.00E-26 ydcR (b1439) 6.00E-15 R08553 (R) IV ysaA (b3573) 4.00E-5 The second category of SMILEY solutions to be investigated in detail was all optimal solutions that fixed more false negatives...”
- “...-ketoglutarate. Two candidate genes with high sequence homology to known genes from other organisms, ydbL (b0600) and ydcR (b1439), were found. The third category to be investigated consisted of the suboptimal solutions that fixed at least one false negative while producing no new false positives. A...”
- EFICAz: a comprehensive approach for accurate genome-scale enzyme function inference
Tian, Nucleic acids research 2004 - “...corresponding partial three digit EC number (e.g. KEGG annotates b0600 as EC 2.6.1.1, which we find difficult to distinguish from EC 2.6.1.57). Third, the EC...”
- Combined, functional genomic-biochemical approach to intermediary metabolism: interaction of acivicin, a glutamine amidotransferase inhibitor, with Escherichia coli K-12
Smulski, Journal of bacteriology 2001 - “...Fold induction b0305 b0358 b0458 b0485 b0572 b0573 b0581 b0600 b0607 b0643 b0662 b0707 b0753 b0786 b0789 b0790 b0800 b0806 b0836 b0865 b0897 b0964 b0966 b1003...”
- “...b2135 b2299 b0305 b0358 b0458 b0485 b0572 b0573 b0581 b0600 b0607 b0643 b0662 b0707 b0753 b0786 b0789 b0790 b0800 b0806 b0836 b0865 b0897 b0964 b0966 b1003...”
- Knowns and Unknowns of Vitamin B6 Metabolism in Escherichia coli
Tramonti, EcoSal Plus 2021 (secret) - Kynurenine aminotransferase and glutamine transaminase K of Escherichia coli: identity with aspartate aminotransferase
Han, The Biochemical journal 2001 (secret)
S0520 putative aminotransferase from Shigella flexneri 2a str. 2457T
32% identity, 92% coverage
- A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
Wuyts, Applied microbiology and biotechnology 2015 - “...isolates related to two different outbreaks with the MOL-PCR assay. The first outbreak included isolates S0520 up to S0524 with S0525 as out-group; the second outbreak consisted of isolates S0526 up to S0530 with isolates S0531 and S0532 as out-group. The developed subtyping assay assigned identical...”
- “...to two different outbreaks Sample Serovar MOL-PCR profile MOL-PCR in-house code Phage type MLVA profile S0520 a Typhimurium 1.9110 7 -3.2610 10 -1 21-19-1 DT195 3-12-10-NA-311 S0521 a Typhimurium 1.9110 7 -3.2610 10 -1 21-19-1 DT195 3-12-10-NA-311 S0522 a Typhimurium 1.9110 7 -3.2610 10 -1 21-19-1...”
B7Z4W5 Cysteine conjugate-beta lyase cytoplasmic (Glutamine transaminase K, kyneurenine aminotransferase), isoform CRA_b from Homo sapiens
NP_001274319 kynurenine--oxoglutarate transaminase 1 isoform c from Homo sapiens
33% identity, 59% coverage
- Multiplexed, Quantitative Workflow for Sensitive Biomarker Discovery in Plasma Yields Novel Candidates for Early Myocardial Injury.
Keshishian, Molecular & cellular proteomics : MCP 2015 - Upregulation of the Glutaminase II Pathway Contributes to Glutamate Production upon Glutaminase 1 Inhibition in Pancreatic Cancer.
Udupa, Proteomics 2019 - GeneRIF: genetic suppression of glutamine transaminase K (GTK), a key enzyme of the glutaminase II pathway, leads to the complete inhibition of pancreatic tumorigenesis in vivo unveiling GTK as a new metabolic target for cancer therapy
- The presence of kynurenine aminotransferases in the human cornea: Evidence from bioinformatics analysis of gene expression and immunohistochemical staining.
Matysik-Woźniak, Molecular vision 2017 - GeneRIF: Immunohistochemical analysis revealed the presence of KAT I, II, and III in all examined corneal sections.
- New insights into the metabolism of organomercury compounds: mercury-containing cysteine S-conjugates are substrates of human glutamine transaminase K and potent inactivators of cystathionine γ-lyase.
Bridges, Archives of biochemistry and biophysics 2012 - GeneRIF: CH(3)Hg-S-Cys and Cys-S-Hg-S-Cys are substrates and reversible inhibitors of GTK.
- Substrate specificity of human glutamine transaminase K as an aminotransferase and as a cysteine S-conjugate beta-lyase.
Cooper, Archives of biochemistry and biophysics 2008 - GeneRIF: alpha-keto acids generated by transamination/l-amino acid oxidase activity of the two catechol cysteine S-conjugates are unstable
- Preliminary evidence for a link between schizophrenia and NMDA-glycine site receptor ligand metabolic enzymes, d-amino acid oxidase (DAAO) and kynurenine aminotransferase-1 (KAT-1).
Kapoor, Brain research 2006 (PubMed)- GeneRIF: We found elevated cerebellar KAT-1 activities in post-mortem brain samples from schizophrenic versus normal individuals.
- The role of glutamine transaminase K (GTK) in sulfur and alpha-keto acid metabolism in the brain, and in the possible bioactivation of neurotoxicants.
Cooper, Neurochemistry international 2004 (PubMed)- GeneRIF: major biological roles of glutamine transaminase K are to maintain low levels of phenylpyruvate and to close the methionine salvage pathway--REVIEW
CCBL1 / Q16773 kynurenine aminotransferase I subunit (EC 2.6.1.117; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.64) from Homo sapiens (see 6 papers)
KAT1_HUMAN / Q16773 Kynurenine--oxoglutarate transaminase 1; Cysteine-S-conjugate beta-lyase; Glutamine transaminase K; GTK; Glutamine--phenylpyruvate transaminase; Kynurenine aminotransferase 1; Kynurenine aminotransferase I; KATI; Kynurenine--oxoglutarate transaminase I; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.64 from Homo sapiens (Human) (see 4 papers)
Q16773 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Homo sapiens (see 8 papers)
33% identity, 72% coverage
- function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others (PubMed:19338303, PubMed:28097769). Also metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites (PubMed:7883047). Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (PubMed:7883047).
catalytic activity: L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O (RHEA:65560)
catalytic activity: 3-phenylpyruvate + L-glutamine = 2-oxoglutaramate + L- phenylalanine (RHEA:17593)
catalytic activity: an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+) (RHEA:18121)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - MMP28 recruits M2-type tumor-associated macrophages through MAPK/JNK signaling pathway-dependent cytokine secretion to promote the malignant progression of pancreatic cancer.
Dong, Journal of experimental & clinical cancer research : CR 2025 - The Biology and Biochemistry of Kynurenic Acid, a Potential Nutraceutical with Multiple Biological Effects.
Alves, International journal of molecular sciences 2024 - “...KYNA synthesis, we consider each in turn. 4.4. KAT1 The main isoform of KAT1 (Uniprot Q16773) has 422 amino acids, a broad substrate specificity as an aminotransferase and also catalyzes -lyase reactions using several cysteine S -conjugates as substrates. It exhibits a preference for glutamine as...”
- Proteome profiling identifies circulating biomarkers associated with hepatic steatosis in subjects with Prader-Willi syndrome
Pascut, Frontiers in endocrinology 2023 - “...0.002 ADGRE2 Q9UHX3 Olink Metabolism 5.40 (5.14 - 5.68) 5.66 (5.59 - 5.90) 0.036 KYAT1 Q16773 Olink Metabolism 6.24 (5.70 - 6.62) 6.57 (6.21 - 7.51) 0.015 MCFD2 Q8NI22 Olink Metabolism 2.38 (2.14 - 2.51) 2.60 (2.40 - 2.86) 0.020 SEMA3F Q13275 Olink Metabolism 4.05 (3.80...”
- “...3.9 0.019 ADGRE2 Q9UHX3 5.39 (5.14-5.67) 5.50 (5.15-5.69) 5.73 (5.60-6.13) 5.85 (5.66-6.16) 1.1 0.02 KYAT1 Q16773 6.24 (5.69-6.62) 6.39 (5.97-7.81) 6.36 (6.13-6.80) 7.45 (7.32-8.26) 5.3 0.02 MCFD2 Q8NI22 2.38 (2.14-2.51) 2.40 (2.24-2.68) 2.60 (2.43-2.81) 2.86 (3.01-2.67) 0.8 0.02 SEMA3F Q13275 4.05 (3.80-4.19) 4.15 (3.98-4.27) 4.37 (4.19-4.57)...”
- Human amygdala involvement in Alzheimer's disease revealed by stereological and dia-PASEF analysis.
Gonzalez-Rodriguez, Brain pathology (Zurich, Switzerland) 2023 - “...Importin subunit alpha4 0.52 0.0167 Q9NTM9 CUTC_HUMAN CUTC Copper homeostasis protein cutC homolog 0.52 0.0427 Q16773 KAT1_HUMAN KYAT1 Kynurenineoxoglutarate transaminase 1 0.52 0.0048 O76094 SRP72_HUMAN SRP72 Signal recognition particle subunit SRP72 0.52 0.0355 Q9H9Q2 CSN7B_HUMAN COPS7B COP9 signalosome complex subunit 7b 0.52 0.0287 O95292 VAPB_HUMAN VAPB...”
- Global analysis of HBV-mediated host proteome and ubiquitylome change in HepG2.2.15 human hepatoblastoma cell line.
Yuan, Cell & bioscience 2021 - “...ligase UBR7 UBR7 0.479 Down Q13616 Cullin-1 CUL1 0.410 Down Q13619 Cullin-4A CUL4A 0.267 Down Q16773 Kynurenineoxoglutarate transaminase 1 CCBL1 0.319 Down Q9Y4X5 E3 ubiquitin-protein ligase ARIH1 ARIH1 0.476 Down Q14527 Helicase-like transcription factor HLTF 0.200 Down Q8IU81 Interferon regulatory factor 2-binding protein 1 IRF2BP1 2.531...”
- A Novel Network Pharmacology Strategy to Decode Metabolic Biomarkers and Targets Interactions for Depression.
Gao, Frontiers in psychiatry 2020 - “...Hydrolase, Serine esterase, Transferase P06276 Cholinesterase 2 Hydrolase, Serine esterase Q13510 Acid ceramidase 2 Hydrolase Q16773 Kynurenineoxoglutarate transaminase 1 2 Aminotransferase, Lyase, Transferase Q5QJU3 Alkaline ceramidase 2 2 Hydrolase Q8TDN7 Alkaline ceramidase 1 2 Hydrolase Q969I3 Glycine N-acyltransferase-like protein 1 2 Acyltransferase, Transferase Q9HCG7 Non-lysosomal glucosylceramidase...”
- THE CONCISE GUIDE TO PHARMACOLOGY 2017/18: Enzymes.
Alexander, British journal of pharmacology 2017 - “...lyase LCysteine:2oxoglutarate aminotransferase 3Mercaptopyruvate sulfurtransferase HGNC, UniProt CBS , P35520 CTH , P32929 KYAT1 , Q16773 MPST , P25325 EC number 4.2.1.22 4.4.1.1 4.4.1.13 2.8.1.2 Common abreviation CBS CSE CAT MPST Endogenous substrates Lcysteine ( K m 610 3 M) [ 81 ], Lhomocysteine Lcysteine Lcysteine...”
- The Concise Guide to PHARMACOLOGY 2015/16: Enzymes.
Alexander, British journal of pharmacology 2015 - “...abreviation CBS CSE CAT MPST HGNC, UniProt CBS , P35520 CTH , P32929 CCBL1 , Q16773 MPST , P25325 EC number 4.2.1.22 4.4.1.1 4.4.1.13 2.8.1.2 Endogenous substrates Lcysteine ( K m 6 10 3 M) [ 74 ], Lhomocysteine Lcysteine Lcysteine 3mercaptopyruvic acid ( K m...”
- More
PFREUD_23330 pyridoxal phosphate-dependent aminotransferase from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
32% identity, 89% coverage
D2Z0I0 alanine-glyoxylate transaminase (EC 2.6.1.44) from Hydrogenobacter thermophilus (see paper)
32% identity, 88% coverage
3fvsB / Q16773 Human kynurenine aminotransferase i in complex with glycerol (see paper)
33% identity, 73% coverage
OCC_03788 pyridoxal phosphate-dependent aminotransferase from Thermococcus litoralis DSM 5473
Q7LYW0 Alanine aminotransferase-like protein from Thermococcus litoralis
30% identity, 91% coverage
OENOO_47017 aromatic amino acid specific aminotransferase from Oenococcus oeni ATCC BAA-1163
30% identity, 91% coverage
DAPAT_SYNFM / A0LEA5 LL-diaminopimelate aminotransferase; DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase; EC 2.6.1.83 from Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) (see paper)
A0LEA5 LL-diaminopimelate aminotransferase (EC 2.6.1.83) from Syntrophobacter fumaroxidans (see paper)
YP_844192 aminotransferase, class I and II from Syntrophobacter fumaroxidans MPOB
31% identity, 90% coverage
- function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL- diaminopimelate. Can also use m-DAP instead of LL-DAP as the amino- group donor.
catalytic activity: (2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)- 2,3,4,5-tetrahydrodipicolinate + L-glutamate + H2O + H(+) (RHEA:23988)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer. - Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase
Nachar, Frontiers in microbiology 2012 - “...burtonii DSM 6242 (YP_565702), Methanosarcina barkeri Fusaro (YP_306095), Methanosarcina acetivorans C2A (NP_616639), Syntrophobacter fumaroxidans MPOB (YP_844192), Planctomyces brasiliensis DSM 5305 (YP_004269630), Planctomyces limnophilus DSM 3776 (YP_003632005), Verrucomicrobiae bacterium DG1235 (ZP_05058547). The accession number for the Yersinia pseudotuberculosis IP 32953 aspartate aminotransferase (AspC) is (CAH20674). A homology...”
MM2649 Aspartate aminotransferase from Methanosarcina mazei Goe1
30% identity, 90% coverage
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...acetivorans, MA1712; Methanosarcina mazei, MM2649; Methanosaeta, Mthe0801; Methanothermobacter, MTH52; Corynebacterium 1, NCgl1343; Corynebacterium...”
- Lysine-2,3-aminomutase and beta-lysine acetyltransferase genes of methanogenic archaea are salt induced and are essential for the biosynthesis of Nepsilon-acetyl-beta-lysine and growth at high salinity
Pflüger, Applied and environmental microbiology 2003 - “...reductase (MM1202), succinyl diaminopimelate aminotransferase (MM2649), and diaminopimelate decarboxylase (MM1885) could be identified. -Lysine is proposed...”
Q8SXC2 kynurenine--oxoglutarate transaminase from Drosophila melanogaster
31% identity, 73% coverage
SSU05_0022 aromatic amino acid aminotransferase from Streptococcus suis 05ZYH33
30% identity, 89% coverage
Ferp_0809 LL-diaminopimelate aminotransferase from Ferroglobus placidus DSM 10642
30% identity, 91% coverage
- Synonymous codon bias and functional constraint on GC3-related DNA backbone dynamics in the prokaryotic nucleoid
Babbitt, Nucleic acids research 2014 - “...Metho_1006 Methanospirillum hungatei JF-1 Mhun_2943 Methanobacterium thermoautotrophicum str. Delta H* Mth_52 Ferroglobus placidus DSM 10642 Ferp_0809 Bacteria Bacteroides fragilis BF2643 Chlamydia trachomatis D(s)2923 CtraD_010100002050 Clostridium thermocellum DSM 2360 ClothDRAFT_2722 Ethanoligenens harbinense YUAN-3 Ethha_0920 Faecalibacterium prausnitzii M21 FAEPRAM212_00561 Geopsychrobacter electrodiphilus DSM 16401 D888DRAFT_2709 Prevotella histicola F0411 HMPREF9138_01762...”
XP_006498378 kynurenine--oxoglutarate transaminase 1 isoform X1 from Mus musculus
36% identity, 45% coverage
XP_008759880 kynurenine--oxoglutarate transaminase 1 isoform X1 from Rattus norvegicus
34% identity, 67% coverage
Q8TQ40 LL-diaminopimelate aminotransferase (EC 2.6.1.83) from Methanosarcina acetivorans (see paper)
MA1712, NP_616639 aspartate aminotransferase from Methanosarcina acetivorans C2A
30% identity, 90% coverage
- Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase
Nachar, Frontiers in microbiology 2012 - “...Dehalococcoides CBDB1 (YP_307791), Methanococcoides burtonii DSM 6242 (YP_565702), Methanosarcina barkeri Fusaro (YP_306095), Methanosarcina acetivorans C2A (NP_616639), Syntrophobacter fumaroxidans MPOB (YP_844192), Planctomyces brasiliensis DSM 5305 (YP_004269630), Planctomyces limnophilus DSM 3776 (YP_003632005), Verrucomicrobiae bacterium DG1235 (ZP_05058547). The accession number for the Yersinia pseudotuberculosis IP 32953 aspartate aminotransferase (AspC)...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...Archaeoglobus, AF0409; Methanosarcina acetivorans, MA1712; Methanosarcina mazei, MM2649; Methanosaeta, Mthe0801; Methanothermobacter, MTH52; Corynebacterium...”
PH1322 aspartate aminotransferase from Pyrococcus horikoshii OT3
28% identity, 90% coverage
KAT1_RAT / Q08415 Kynurenine--oxoglutarate transaminase 1; Kynurenine--oxoglutarate transaminase I; Cysteine-S-conjugate beta-lyase; Glutamine transaminase K; GTK; Glutamine--phenylpyruvate transaminase; Kynurenine aminotransferase 1; Kynurenine aminotransferase I; KATI; EC 2.6.1.7; EC 4.4.1.13; EC 2.6.1.64 from Rattus norvegicus (Rat) (see 4 papers)
Q08415 kynurenine-oxoglutarate transaminase (EC 2.6.1.7) from Rattus norvegicus (see 5 papers)
34% identity, 72% coverage
- function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others (PubMed:2072101, PubMed:6783036, PubMed:7796908). Metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond (PubMed:8502223).
catalytic activity: L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O (RHEA:65560)
catalytic activity: 3-phenylpyruvate + L-glutamine = 2-oxoglutaramate + L- phenylalanine (RHEA:17593)
catalytic activity: an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+) (RHEA:18121)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
LFER_138 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Limosilactobacillus fermentum
31% identity, 88% coverage
BB5012 probable aminotransferase from Bordetella bronchiseptica RB50
33% identity, 84% coverage
BCAL0049 putative aminotransferase from Burkholderia cenocepacia J2315
34% identity, 89% coverage
BP3870 probable aminotransferase from Bordetella pertussis Tohama I
32% identity, 85% coverage
BH1060 hypothetical protein from Bacillus halodurans C-125
30% identity, 89% coverage
BA2899 aminotransferase, classes I and II from Bacillus anthracis str. Ames
30% identity, 89% coverage
BPP4424 probable aminotransferase from Bordetella parapertussis 12822
33% identity, 84% coverage
BAS2700 aminotransferase, classes I and II from Bacillus anthracis str. Sterne
30% identity, 89% coverage
OENOO_60020 aromatic amino acid aminotransferase from Oenococcus oeni ATCC BAA-1163
29% identity, 95% coverage
DAPAT_MOOTA / Q2RK33 LL-diaminopimelate aminotransferase; DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase; EC 2.6.1.83 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
Q2RK33 LL-diaminopimelate aminotransferase (EC 2.6.1.83) from Moorella thermoacetica (see paper)
30% identity, 90% coverage
- function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL- diaminopimelate. Is also able to catalyze the reverse reaction in vitro, i.e. the transamination of LL-diaminopimelate with 2- oxoglutarate to produce tetrahydrodipicolinate and glutamate. Can also use m-DAP instead of LL-DAP as the amino-group donor, and oxaloacetate instead of 2-oxoglutarate as the amino-group acceptor.
catalytic activity: (2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)- 2,3,4,5-tetrahydrodipicolinate + L-glutamate + H2O + H(+) (RHEA:23988)
cofactor: pyridoxal 5'-phosphate
subunit: Homodimer.
Q3MDN5 LL-diaminopimelate aminotransferase (EC 2.6.1.83) from Trichormus variabilis (see paper)
31% identity, 93% coverage
D805_0525 pyridoxal phosphate-dependent aminotransferase from Bifidobacterium thermophilum RBL67
33% identity, 81% coverage
- Bifidobacterium thermophilum RBL67 impacts on growth and virulence gene expression of Salmonella enterica subsp. enterica serovar Typhimurium
Tanner, BMC microbiology 2016 - “...1.5E-15 D805_0512 hypothetical protein 2.01 2.39 0.03026 D805_0524 D-lactate dehydrogenase (EC 1.1.1.28) 2.85 3.15 0.00049 D805_0525 Aspartate aminotransferase (EC 2.6.1.1) 1.91 3.11 0.01352 D805_0652 Oligopeptide transport system permease protein OppC (EC 3.A.1.5.1) 2.25 2.47 0.00227 D805_0656 hypothetical protein 1.57 3.01 0.01267 D805_0665 hypothetical protein 1.18 5.85...”
- “...A putative operon encoding glycosyltransferases (ORF D805_0351-D805_0356), three genes involved in amino acid metabolism (D805_0341, D805_0525 and D805_1313), including the glutamate producing enzyme aspartate aminotransferase (EC 2.6.1.1) and two metal transporters (D805_0345 and D805_0885) were higher expressed. Mapping the co-culture reads to the Salmonella Typhimurium LT2...”
BASU_3402 pyridoxal phosphate-dependent aminotransferase from Bacillus velezensis UCMB5113
31% identity, 90% coverage
YP_306095 aspartate aminotransferase from Methanosarcina barkeri str. fusaro
30% identity, 90% coverage
- Genomic and Biochemical Analysis of the Diaminopimelate and Lysine Biosynthesis Pathway in Verrucomicrobium spinosum: Identification and Partial Characterization of L,L-Diaminopimelate Aminotransferase and UDP-N-Acetylmuramoylalanyl-D-glutamyl-2,6-meso-Diaminopimelate Ligase
Nachar, Frontiers in microbiology 2012 - “...Methanospirillum hungatei JF1 (YP_504354), Dehalococcoides CBDB1 (YP_307791), Methanococcoides burtonii DSM 6242 (YP_565702), Methanosarcina barkeri Fusaro (YP_306095), Methanosarcina acetivorans C2A (NP_616639), Syntrophobacter fumaroxidans MPOB (YP_844192), Planctomyces brasiliensis DSM 5305 (YP_004269630), Planctomyces limnophilus DSM 3776 (YP_003632005), Verrucomicrobiae bacterium DG1235 (ZP_05058547). The accession number for the Yersinia pseudotuberculosis IP...”
TK1094 alanine aminotransferase from Thermococcus kodakaraensis KOD1
28% identity, 94% coverage
- TK2268 encodes the major aminotransferase involved in the conversion from oxaloacetic acid to aspartic acid in <i>Thermococcus kodakarensis</i>
Su, Applied and environmental microbiology 2025 (PubMed)- “...aspartate is unknown. Here, four Class I aminotransferases in T. kodakarensis encoded by TK0186, TK0548, TK1094, and TK2268 were examined to identify the enzyme(s) responsible for the conversion of oxaloacetate to aspartate. Among the four proteins, the TK2268 protein (TK2268p) was the only protein to recognize...”
- Removal of phosphoglycolate in hyperthermophilic archaea
Michimori, Proceedings of the National Academy of Sciences of the United States of America 2024 - “...respectively. ( D ) AGAT activity in cell extracts from KU216, TK0186, TK0548, TK2268, and TK1094 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. Activity measurements were carried out as described in the Methods...”
- “...the T. kodakarensis proteome resulted in the identification of multiple proteins (encoded by TK0186, TK2268, TK1094, TK0548, TK0260, and TK0250) with varying degrees of homology, suggesting that multiple proteins might exhibit aminotransferase activity with glyoxylate as the amino acceptor. Among the six candidate genes, TK0250 and...”
- Biochemical and genetic examination of two aminotransferases from the hyperthermophilic archaeon Thermococcus kodakarensis
Su, Frontiers in microbiology 2023 - “...activity toward Phe and Tyr ( Ward et al., 2000 ). A genetic analysis on TK1094 from T. kodakarensis (G3) suggested a role in the conversion between pyruvate and Ala ( Kanai et al., 2015 ). The PF1702 protein (G4), referred to as AspAT, displays highest...”
- “...His 2-OG, (Pyr) This study TK2268 G2 Aspartate aminotransferase Glu, Asp 2-OG, (Pyr) This study TK1094 G3 (Alanine aminotransferase) Kanai et al. (2015) TK0260 G4 Aspartate aminotransferase TK0186 G6 (2-Aminoadipate transaminase) TK0250 G7 (Histidinol-phosphate aminotransferase) TK0864 G8 (Threonine- O -3-phosphate decarboxylase) II TK0275 LysW--L-lysine aminotransferase Yoshida...”
- Genetic examination of initial amino acid oxidation and glutamate catabolism in the hyperthermophilic archaeon Thermococcus kodakarensis
Yokooji, Journal of bacteriology 2013 - “...have observed that transcript levels of TK1094, encoding alanine aminotransferase, and TK0186, encoding multiple-substrate aminotransferase, increase more than...”
- Deletion of alternative pathways for reductant recycling in Thermococcus kodakarensis increases hydrogen production
Santangelo, Molecular microbiology 2011 - “...are available to re-oxidize reduced cofactors. Alanine is produced from pyruvate by an alanine aminotransferase (TK1094; Fig. 1 ; grey) that concomitantly generates 2-oxoglutarate from glutamate. Regeneration of glutamate by a glutamate dehydrogenase (TK1431) then oxidizes NADPH to NADP ( Fukui et al. , 2005 )....”
- “...et al. , 2010 ; Santangelo and Reeve, 2010 ) illustrated for the deletion of TK1094 ( Fig. 2 ), was repeated to generate all the T. kodakarensis strains listed in Table 1 . For each strain, the genes/operons shown in Fig. 3 were precisely deleted....”
- Distinct physiological roles of the three [NiFe]-hydrogenase orthologs in the hyperthermophilic archaeon Thermococcus kodakarensis
Kanai, Journal of bacteriology 2011 - “...for disruption of hyhBGSL (TK2072 to TK2069), aat (TK1094), mbhJKL (TK2089 to TK2091), and mbxJKL (TK1217 to TK1215) in T. kodakarensis (named pUDHY1, pUDAT1,...”
- “...In this study using MA-YT-Pyr medium, disruption of TK1094, encoding AlaAT, resulted in a significant decrease in alanine formation, indicating that this gene...”
- Complete genome sequence of the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 and comparison with Pyrococcus genomes
Fukui, Genome research 2005 - “...is also converted to alanine by alanine aminotransferase (TK1094) using glutamate as an amino donor, which is reductively regenerated from 2-oxoglutarate by...”
AF_2366 pyridoxal phosphate-dependent aminotransferase from Archaeoglobus fulgidus DSM 4304
29% identity, 90% coverage
- A novel methoxydotrophic metabolism discovered in the hyperthermophilic archaeon Archaeoglobus fulgidus
Welte, Environmental microbiology 2021 - “...2oxoglutarate/2oxoacid ferredoxin oxidoreductase KorABDG (AF_046871), glutamate synthase GltB13 (AF_09524), aspartate aminotransferase AspBC (AF_0409, AF_1623, AF_2129, AF_2366, AF_1417), isocitrate dehydrogenase Icd (AF_0647), aconitase Acn (AF_1963), citrate synthase CitZ (AF_1340), sulfate adenylyltransferase Sat (AF_1667), adenylylsulfate reductase AprAB (AF_166970), dissimilatory sulfite reductase DsrAB (AF_04234), DsrC (AF_2228), quinonemodifying oxidoreductase QmoABC...”
YPO0623 putative aminotransferase from Yersinia pestis CO92
y3555 aspartate aminotransferase from Yersinia pestis KIM
30% identity, 79% coverage
- Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in Yersinia pestis
Jin, Frontiers in cellular and infection microbiology 2023 - “...10713766 10.3389/fcimb.2023.1288371 Cellular and Infection Microbiology Original Research Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in Yersinia pestis Jin Junyan 1 Xiao Liting 1 2 Wu Yarong 1 Sun Zhulin 1 Xiong Ziyao 1 3 Li Yanbing 1 4 Zhao Yanting...”
- “...from Yersinia pseudotuberculosis approximately 7,400 years ago. We observed unusually frequent mutations in Y. pestis YPO0623, mostly resulting in protein translation termination, which implies a strong natural selection. These mutations were found in all phylogenetic lineages of Y. pestis , and there was no apparent pattern...”
- Small Insertions and Deletions Drive Genomic Plasticity during Adaptive Evolution of Yersinia pestis
Wu, Microbiology spectrum 2022 - “...of indels along the genome, we found 5 genes, sspA , rpoS , rnd , YPO0623, and YPO0624, with extraordinarily more indels enriched than common genes ( Fig.1D ). Surprisingly, except for YPO0623, the indel-variants, as well as substitutions, in these 4 genes, mainly occurred in...”
- “...Ribonuclease D 1,122 7/7 3/3 YPO3355/ rpoS RNA polymerase sigma factor RpoS 999 10/11 4/6 YPO0623 Aminotransferase 1,233 1/12 0/5 We then recalled both indels and SNPs for 119 genomes isolated from Brazil ( 23 ), which all belonged to a sublineage of 1.ORI2 (Fig. S3)....”
- Temporal global changes in gene expression during temperature transition in Yersinia pestis
Motin, Journal of bacteriology 2004 - “...(putA), proline permease (putP), and a putative aminotransferase (YPO0623). Some of these enzymes account for the bulk of released metabolic ammonia via...”
- Characterization of an aspartate aminotransferase encoded by YPO0623 with frequent nonsense mutations in Yersinia pestis
Jin, Frontiers in cellular and infection microbiology 2023 - “...promoting the growth of the bacterium. Notably, a recent study found that YPO0623 (referred as y3555 in strain KIM10+), in conjunction with other genes, plays a crucial role in optimizing aromatic amino acid metabolism and is induced under conditions of hyperosmotic salinity stress. It might contribute...”
- Putative Horizontally Acquired Genes, Highly Transcribed during Yersinia pestis Flea Infection, Are Induced by Hyperosmotic Stress and Function in Aromatic Amino Acid Metabolism
Martínez-Chavarría, Journal of bacteriology 2020 - “...two physiologically disparate host environments. In fleas competent to transmit Y. pestis , low-GC-content genes y3555 , y3551, and y3550 are highly transcribed, suggesting that these genes have a highly prioritized role in flea infection. Here, we demonstrate that y3555 , y3551, and y3550 are transcribed...”
- The Diverse Roles of the Global Transcriptional Regulator PhoP in the Lifecycle of Yersinia pestis
Fukuto, Pathogens (Basel, Switzerland) 2020 - “...acid metabolism and are stimulated by hyperosmotic salinity [ 67 ]. Together with phoP and y3555 , y3553 and y3550 rank among the mostly highly expressed genes in wild-type Y. pestis during flea blockage [ 34 ]. Another example is that of the YhcN family genes...”
- Differential Gene Expression Patterns of Yersinia pestis and Yersinia pseudotuberculosis during Infection and Biofilm Formation in the Flea Digestive Tract
Chouikha, mSystems 2019 - “...LPS extracts (see Fig.S1 in the supplemental material). Deletion of nhaC locus genes y3550 to y3555 in Y. pestis KIM6+ was accomplished using a lambda Red recombinase system ( 88 ). PCR primers were designed to amplify a kanamycin resistance gene and to incorporate approximately 50...”
- “...genes were deleted, including approximately 200 bp upstream of y3550 and 100 bp downstream of y3555. YPTB0553 ( lsrR ) and YPTB3828 (PDE4) genes were amplified by PCR and cloned into pCR2.1-TOPO (Invitrogen). Deletion of YTPB3828 was accomplished using inverse PCR primers to delete amino acids...”
- Global gene expression profiling of Yersinia pestis replicating inside macrophages reveals the roles of a putative stress-induced operon in regulating type III secretion and intracellular cell division
Fukuto, Infection and immunity 2010 - “...YPO1366 y1607 y0655 y2882 y1189 y1041 y0310 y1607 YPO1423a y3555 y0509 y0016 y0463 y0655 y1821 y2881 y2125 y3804 y2236 y0015 YPO3681 y3836 y2591 50S ribosomal...”
DAPC_MYCTU / P9WPZ5 Probable N-succinyldiaminopimelate aminotransferase DapC; DAP-AT; EC 2.6.1.17 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
P9WPZ5 succinyldiaminopimelate transaminase (EC 2.6.1.17) from Mycobacterium tuberculosis (see paper)
Rv0858c hypothetical protein from Mycobacterium tuberculosis H37Rv
36% identity, 88% coverage
- function: Involved in the lysine biosynthetic pathways. It catalyzes the transfer of an amino group from L-glutamate to N-succinyl-2-l- amino-6-oxoheptanedioate (N-succinyl-2-l-amino-6-ketopimelate) in a PLP-dependent reaction, yielding as products N-succinyl-l-2,6- diaminoheptanedioate (N-succinyl-diaminopimelate) and 2-oxoglutarate (Probable).
catalytic activity: N-succinyl-(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2-succinylamino-6-oxoheptanedioate + L-glutamate (RHEA:11960)
cofactor: pyridoxal 5'-phosphate Note=Binds 1 pyridoxal phosphate per subunit
subunit: Homodimer. - The efflux pumps Rv1877 and Rv0191 play differential roles in the protection of Mycobacterium tuberculosis against chemical stress
Sao, Frontiers in microbiology 2024 - “...involved in the biosynthesis of arginine from glutamate ( Tiwari et al., 2018 ) and rv0858c encoding N-succinyldiaminopimelate aminotransferase (DapC; Weyand et al., 2006 ) which were downregulated. Also, rv3290c (encoding lysine 6-aminotransferase; Mani Tripathi and Ramachandran, 2006 ) that was shown to be contribute to...”
- “...S. ( 2006 ). Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapC (Rv0858c) from Mycobacterium tuberculosis . Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62 , 794 797 . doi: 10.1107/S1744309106026753 , PMID: 16880560 Wietzerbin J. Lederer F. Petit J. F. (...”
- The cell envelope glycoconjugates of Mycobacterium tuberculosis
Angala, Critical reviews in biochemistry and molecular biology 2014 - “...of eight reactions involving the enzymes Ask (Rv3709c), Asd (Rv3708c), DapA (Rv2753c), DapB (Rv2773c), DapC (Rv0858c), DapD (Rv1201c), DapE (Rv1202) and DapF (Rv2726c). As in other bacteria, the fully assembled sugar-peptide moiety of the Parks nucleotide is then transferred to a lipid carrier by the phospho-...”
- A central role for aspartate in Mycobacterium tuberculosis physiology and virulence
Gouzy, Frontiers in cellular and infection microbiology 2013 - “...be required for M. tuberculosis virulence. Noteworthy, AspB and AspC possess two homologue proteins, called Rv0858c and Rv1178, that are predicted to act as aspartate aminotransferases, and that might provide alternative pathways for aspartate-derived nitrogen assimilation in the TB bacillus (Cole et al., 1998 ). Finally,...”
- Proteomic definition of the cell wall of Mycobacterium tuberculosis
Wolfe, Journal of proteome research 2010 - “...CONSERVED HYPOTHETICAL PROTEIN 10 V B Rv0771 Rv0771 POSSIBLE 4-CARBOXYMUCONOLACTONE DECARBOXYLASE (CMD) 7 II.B.6 D Rv0858c Rv0858c PROBABLE AMINOTRANSFERASE 7 IV.H A Rv0892 Rv0892 PROBABLE MONOOXYGENASE 7 I.B.7 B Rv0899 ompA OUTER MEMBRANE PROTEIN A OMPA 3 II.C.2 D Rv0902c prrB TWO COMPONENT SENSOR HISTIDINE KINASE...”
- “...yes ( 16 , 19 ) Rv0622 Rv0622 x Rv0830 Rv0830 x ( 46 ) Rv0858c Rv0858c x Rv0892 Rv0892 x Rv0902c prrB x Rv0928 pstS3 x yes yes ( 16 , 45 ) Rv0934 pstS1 x x x yes yes yes ( 42 , 19...”
- The three-dimensional structure of N-succinyldiaminopimelate aminotransferase from Mycobacterium tuberculosis
Weyand, Journal of molecular biology 2007 (PubMed)- “...three-dimensional structure of N-succinyldiaminopimelate aminotransferase (DapC, DAP-AT, Rv0858c) to a resolution of 2.0. This structure is the first DAP-AT...”
- “...to be present. The reaction catalysed by Mtb-DAP-AT (Rv0858c) is highlighted in bold. (b) Reaction catalysed by Mtb-DAP-AT (Rv0858c). Table 1 Refinement and...”
- Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapC (Rv0858c) from Mycobacterium tuberculosis
Weyand, Acta crystallographica. Section F, Structural biology and crystallization communications 2006 - “...and preliminary X-ray diffraction analysis of DapC (Rv0858c) from Mycobacterium tuberculosis ISSN 1744-3091 Simone Weyand, Georgia Kefala and Manfred S. Weiss*...”
- “...aminotransferase from Mycobacterium tuberculosis (DAP-AT; DapC; Rv0858c) has been cloned, heterologously expressed in Escherichia coli, purified using standard...”
- Genomic approach to identifying the putative target of and mechanisms of resistance to mefloquine in mycobacteria
Danelishvili, Antimicrobial agents and chemotherapy 2005 - “...Rv3466 Rv3467 Intermediary metabolism and respiration Rv0334 Rv0389 Rv0777 Rv0858c Rv1090 Rv1092 Rv1178 rmlA purT purB celA2b coaA Fold change 2.5 2 2.3 2 2.2 2...”
- Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis
Venos, BMC microbiology 2004 - “...while M. tuberculosis has only the one branched-chain aminotransferase, it does contain a coding sequence (Rv0858c) with a high similarity to the B. subtilis ykrV gene product. YkrV was found to be a subfamily If aminotransferase and could also catalyse the conversion of KMTB to Met...”
- “...as the only effective amino donor [ 16 ]. Therefore, it is possible that the Rv0858c gene product might be capable of KMTB transamination. It should be stressed that while the recombinant B. subtilis YkrV could transaminate KMTB with glutamine, B. subtilis cell homogenates did not...”
WP_010273745 pyridoxal phosphate-dependent aminotransferase from Paenibacillus senegalensis JC66
31% identity, 91% coverage
PFREUD_04930 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme from Propionibacterium freudenreichii subsp. shermanii CIRM-BIA1
33% identity, 93% coverage
SCLAV_p1070 pyridoxal phosphate-dependent aminotransferase from Streptomyces clavuligerus
35% identity, 82% coverage
BP1062 probable aminotransferase from Bordetella pertussis Tohama I
32% identity, 90% coverage
PAP_08180 pyridoxal phosphate-dependent aminotransferase from Palaeococcus pacificus DY20341
30% identity, 81% coverage
2o0rA / P9WPZ5 The three-dimensional structure of n-succinyldiaminopimelate aminotransferase from mycobacterium tuberculosis (see paper)
36% identity, 89% coverage
CJJ81176_0186 aminotransferase, classes I and II from Campylobacter jejuni subsp. jejuni 81-176
29% identity, 90% coverage
XNC1_2154 pyridoxal phosphate-dependent aminotransferase from Xenorhabdus nematophila ATCC 19061
27% identity, 90% coverage
- The Global Regulators Lrp, LeuO, and HexA Control Secondary Metabolism in Entomopathogenic Bacteria
Engel, Frontiers in microbiology 2017 - “...XNC1_2039 NRPS 2.68 XNC1_2040 NRPS 2.11 Unknown XNC1_2152 Hypothetical protein 3.67 XNC1_2153 Arginine aminomutase 29.52 XNC1_2154 Aminotransferase 16.99 XNC1_2155 Putative clavaminate synthase 11.40 XNC1_2156 PKS 9.17 XNC1_2157 PKS/NRPS hybrid 5.75 XNC1_2158 Putative membrane protein 5.41 XNC1_2159 Putative 2-dehydropantoate 2-reductase 4.05 XNC1_2160 Putative dioxygenase 2.88 XNC1_2161 NRPS...”
AF_0409 LL-diaminopimelate aminotransferase from Archaeoglobus fulgidus DSM 4304
AF0409 aspartate aminotransferase (aspB-4) from Archaeoglobus fulgidus DSM 4304
31% identity, 91% coverage
- A novel methoxydotrophic metabolism discovered in the hyperthermophilic archaeon Archaeoglobus fulgidus
Welte, Environmental microbiology 2021 - “...(AF_1539/40 & AF_2185/6), 2oxoglutarate/2oxoacid ferredoxin oxidoreductase KorABDG (AF_046871), glutamate synthase GltB13 (AF_09524), aspartate aminotransferase AspBC (AF_0409, AF_1623, AF_2129, AF_2366, AF_1417), isocitrate dehydrogenase Icd (AF_0647), aconitase Acn (AF_1963), citrate synthase CitZ (AF_1340), sulfate adenylyltransferase Sat (AF_1667), adenylylsulfate reductase AprAB (AF_166970), dissimilatory sulfite reductase DsrAB (AF_04234), DsrC (AF_2228),...”
- Synonymous codon bias and functional constraint on GC3-related DNA backbone dynamics in the prokaryotic nucleoid
Babbitt, Nucleic acids research 2014 - “...for each ortholog used in the study. Organism Locus tag Archaea Archaeoglobus fulgidus DSM 4304 AF_0409 Candidatus methanoregulaboonei 6A8 Mboo_2096 Geoarchaeota archaeon OSPB-1 Cren1_8best_scaffolds_00013940 Methanobacterium formicicum DSM 3637 A994_03433 Methanocaldococcus villosus KIN24-T80 MetviDRAFT_0533 Methanocella paludicola SANAE MCPlv_0872 Methanomethylovorans hollandica DSM 15978 Metho_1006 Methanospirillum hungatei JF-1 Mhun_2943...”
- Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis
Liu, Journal of bacteriology 2010 - “...Methanospirillum, Mhun2943; Moorella, Moth0889; Archaeoglobus, AF0409; Methanosarcina acetivorans, MA1712; Methanosarcina mazei, MM2649; Methanosaeta, Mthe0801;...”
TON_0231 alanine aminotransferase from Thermococcus onnurineus NA1
28% identity, 93% coverage
Clocel_3812 pyridoxal phosphate-dependent aminotransferase from Clostridium cellulovorans 743B
27% identity, 90% coverage
- Clostridium cellulovorans Proteomic Responses to Butanol Stress
Costa, Frontiers in microbiology 2021 - “...and Supplementary Table 2 ). In addition, up-regulated proteins include four aminotransferases (Clocel_1948, Clocel_2059, Clocel_2390, Clocel_3812). The role of amino acids in the cellular stress response is a well-known concern. Increase in pyrroline-5-carboxylate reductase and intracellular levels of proline in response to butanol stress has been...”
For advice on how to use these tools together, see
Interactive tools for functional annotation of bacterial genomes.
The PaperBLAST database links 793,807 different protein sequences to 1,259,118 scientific articles. Searches against EuropePMC were last performed on March 13 2025.
PaperBLAST builds a database of protein sequences that are linked
to scientific articles. These links come from automated text searches
against the articles in EuropePMC
and from manually-curated information from GeneRIF, UniProtKB/Swiss-Prot,
BRENDA,
CAZy (as made available by dbCAN),
BioLiP,
CharProtDB,
MetaCyc,
EcoCyc,
TCDB,
REBASE,
the Fitness Browser,
and a subset of the European Nucleotide Archive with the /experiment tag.
Given this database and a protein sequence query,
PaperBLAST uses protein-protein BLAST
to find similar sequences with E < 0.001.
To build the database, we query EuropePMC with locus tags, with RefSeq protein
identifiers, and with UniProt
accessions. We obtain the locus tags from RefSeq or from MicrobesOnline. We use
queries of the form "locus_tag AND genus_name" to try to ensure that
the paper is actually discussing that gene. Because EuropePMC indexes
most recent biomedical papers, even if they are not open access, some
of the links may be to papers that you cannot read or that our
computers cannot read. We query each of these identifiers that
appears in the open access part of EuropePMC, as well as every locus
tag that appears in the 500 most-referenced genomes, so that a gene
may appear in the PaperBLAST results even though none of the papers
that mention it are open access. We also incorporate text-mined links
from EuropePMC that link open access articles to UniProt or RefSeq
identifiers. (This yields some additional links because EuropePMC
uses different heuristics for their text mining than we do.)
For every article that mentions a locus tag, a RefSeq protein
identifier, or a UniProt accession, we try to select one or two
snippets of text that refer to the protein. If we cannot get access to
the full text, we try to select a snippet from the abstract, but
unfortunately, unique identifiers such as locus tags are rarely
provided in abstracts.
PaperBLAST also incorporates manually-curated protein functions:
- Proteins from NCBI's RefSeq are included if a
GeneRIF
entry links the gene to an article in
PubMed®.
GeneRIF also provides a short summary of the article's claim about the
protein, which is shown instead of a snippet.
- Proteins from Swiss-Prot (the curated part of UniProt)
are included if the curators
identified experimental evidence for the protein's function (evidence
code ECO:0000269). For these proteins, the fields of the Swiss-Prot entry that
describe the protein's function are shown (with bold headings).
- Proteins from BRENDA,
a curated database of enzymes, are included if they are linked to a paper in PubMed
and their full sequence is known.
- Every protein from the non-redundant subset of
BioLiP,
a database
of ligand-binding sites and catalytic residues in protein structures, is included. Since BioLiP itself
does not include descriptions of the proteins, those are taken from the
Protein Data Bank.
Descriptions from PDB rely on the original submitter of the
structure and cannot be updated by others, so they may be less reliable.
(For SitesBLAST and Sites on a Tree, we use a larger subset of BioLiP so that every
ligand is represented among a group of structures with similar sequences, but for
PaperBLAST, we use the non-redundant set provided by BioLiP.)
- Every protein from EcoCyc, a curated
database of the proteins in Escherichia coli K-12, is included, regardless
of whether they are characterized or not.
- Proteins from the MetaCyc metabolic pathway database
are included if they are linked to a paper in PubMed and their full sequence is known.
- Proteins from the Transport Classification Database (TCDB)
are included if they have known substrate(s), have reference(s),
and are not described as uncharacterized or putative.
(Some of the references are not visible on the PaperBLAST web site.)
- Every protein from CharProtDB,
a database of experimentally characterized protein annotations, is included.
- Proteins from the CAZy database of carbohydrate-active enzymes
are included if they are associated with an Enzyme Classification number.
Even though CAZy does not provide links from individual protein sequences to papers,
these should all be experimentally-characterized proteins.
- Proteins from the REBASE database
of restriction enzymes are included if they have known specificity.
- Every protein with an evidence-based reannotation (based on mutant phenotypes)
in the Fitness Browser is included.
- Sequence-specific transcription factors (including sigma factors and DNA-binding response regulators)
with experimentally-determined DNA binding sites from the
PRODORIC database of gene regulation in prokaryotes.
- Putative transcription factors from RegPrecise
that have manually-curated predictions for their binding sites. These predictions are based on
conserved putative regulatory sites across genomes that contain similar transcription factors,
so PaperBLAST clusters the TFs at 70% identity and retains just one member of each cluster.
- Coding sequence (CDS) features from the
European Nucleotide Archive (ENA)
are included if the /experiment tag is set (implying that there is experimental evidence for the annotation),
the nucleotide entry links to paper(s) in PubMed,
and the nucleotide entry is from the STD data class
(implying that these are targeted annotated sequences, not from shotgun sequencing).
Also, to filter out genes whose transcription or translation was detected, but whose function
was not studied, nucleotide entries or papers with more than 25 such proteins are excluded.
Descriptions from ENA rely on the original submitter of the
sequence and cannot be updated by others, so they may be less reliable.
Except for GeneRIF and ENA,
the curated entries include a short curated
description of the protein's function.
For entries from BioLiP, the protein's function may not be known beyond binding to the ligand.
Many of these entries also link to articles in PubMed.
For more information see the
PaperBLAST paper (mSystems 2017)
or the code.
You can download PaperBLAST's database here.
Changes to PaperBLAST since the paper was written:
- November 2023: incorporated PRODORIC and RegPrecise. Many PRODORIC entries were not linked to a protein sequence (no UniProt identifier), so we added this information.
- February 2023: BioLiP changed their download format. PaperBLAST now includes their non-redundant subset. SitesBLAST and Sites on a Tree use a larger non-redundant subset that ensures that every ligand is represented within each cluster. This should ensure that every binding site is represented.
- June 2022: incorporated some coding sequences from ENA with the /experiment tag.
- March 2022: incorporated BioLiP.
- April 2020: incorporated TCDB.
- April 2019: EuropePMC now returns table entries in their search results. This has expanded PaperBLAST's database, but most of the new entries are of low relevance, and the resulting snippets are often just lists of locus tags with annotations.
- February 2018: the alignment page reports the conservation of the hit's functional sites (if available from from Swiss-Prot or UniProt)
- January 2018: incorporated BRENDA.
- December 2017: incorporated MetaCyc, CharProtDB, CAZy, REBASE, and the reannotations from the Fitness Browser.
- September 2017: EuropePMC no longer returns some table entries in their search results. This has shrunk PaperBLAST's database, but has also reduced the number of low-relevance hits.
Many of these changes are described in Interactive tools for functional annotation of bacterial genomes.
PaperBLAST cannot provide snippets for many of the papers that are
published in non-open-access journals. This limitation applies even if
the paper is marked as "free" on the publisher's web site and is
available in PubmedCentral or EuropePMC. If a journal that you publish
in is marked as "secret," please consider publishing elsewhere.
Many important articles are missing from PaperBLAST, either because
the article's full text is not in EuropePMC (as for many older
articles), or because the paper does not mention a protein identifier such as a locus tag, or because of PaperBLAST's heuristics. If you notice an
article that characterizes a protein's function but is missing from
PaperBLAST, please notify the curators at UniProt
or add an entry to GeneRIF.
Entries in either of these databases will eventually be incorporated
into PaperBLAST. Note that to add an entry to UniProt, you will need
to find the UniProt identifier for the protein. If the protein is not
already in UniProt, you can ask them to create an entry. To add an
entry to GeneRIF, you will need an NCBI Gene identifier, but
unfortunately many prokaryotic proteins in RefSeq do not have
corresponding Gene identifers.
References
PaperBLAST: Text-mining papers for information about homologs.
M. N. Price and A. P. Arkin (2017). mSystems, 10.1128/mSystems.00039-17.
Europe PMC in 2017.
M. Levchenko et al (2017). Nucleic Acids Research, 10.1093/nar/gkx1005.
Gene indexing: characterization and analysis of NLM's GeneRIFs.
J. A. Mitchell et al (2003). AMIA Annu Symp Proc 2003:460-464.
UniProt: the universal protein knowledgebase.
The UniProt Consortium (2016). Nucleic Acids Research, 10.1093/nar/gkw1099.
BRENDA in 2017: new perspectives and new tools in BRENDA.
S. Placzek et al (2017). Nucleic Acids Research, 10.1093/nar/gkw952.
The EcoCyc database: reflecting new knowledge about Escherichia coli K-12.
I. M. Keeseler et al (2016). Nucleic Acids Research, 10.1093/nar/gkw1003.
The MetaCyc database of metabolic pathways and enzymes.
R. Caspi et al (2018). Nucleic Acids Research, 10.1093/nar/gkx935.
CharProtDB: a database of experimentally characterized protein annotations.
R. Madupu et al (2012). Nucleic Acids Research, 10.1093/nar/gkr1133.
The carbohydrate-active enzymes database (CAZy) in 2013.
V. Lombard et al (2014). Nucleic Acids Research, 10.1093/nar/gkt1178.
The Transporter Classification Database (TCDB): recent advances
M. H. Saier, Jr. et al (2016). Nucleic Acids Research, 10.1093/nar/gkv1103.
REBASE - a database for DNA restriction and modification: enzymes, genes and genomes.
R. J. Roberts et al (2015). Nucleic Acids Research, 10.1093/nar/gku1046.
Deep annotation of protein function across diverse bacteria from mutant phenotypes.
M. N. Price et al (2016). bioRxiv, 10.1101/072470.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory