Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for argA (N-acylglutamate synthase) in L-arginine biosynthesis

Or see other characterized proteins similar to argA

Or see all steps for L-arginine biosynthesis

Or cluster curated proteins matching a keyword

Definition of argA

Curated proteins or TIGRFams with EC 2.3.1.1
Curated proteins matching N-succinylglutamate synthase
UniProt sequence Q8A1A5_BACTN: RecName: Full=GNAT family N-acetyltransferase {ECO:0008006|Google:ProtNLM};
UniProt sequence L0G3H4_ECHVK: SubName: Full=Acetyltransferase, N-acetylglutamate synthase {ECO:0000313|EMBL:AGA80057.1};
Curated sequence A0A0H2X8L7: acetylglutamate kinase (EC 2.7.2.8)
Curated sequence Q8P8J6: acetylglutamate kinase (EC 2.7.2.8)
Ignore hits to G6759-MONOMER when looking for 'other' hits (L-amino acid N-acyltransferase MnaT; L-methionine N-acyltransferase; L-methionine sulfoximine/L-methionine sulfone N-acetyltransferase; L-phenylglycine N-acetyltransferase; EC 2.3.1.-. L-amino acid N-acyltransferase (EC 2.3.1.1). L-amino acid N-acyltransferase (EC 2.3.1.1))
Ignore hits to items matching EC 2.3.1.35 when looking for 'other' hits
Ignore hits to CH_123299 when looking for 'other' hits (arginine biosynthetic enzyme activities)
Curated sequence CH_122594: glutamate N-acetyltransferase (Eurofung)
Ignore hits to Q9I3W7 when looking for 'other' hits (amino-acid N-acetyltransferase (EC 2.3.1.1))
Ignore hits to Q8P8J6 when looking for 'other' hits (acetylglutamate kinase (EC 2.7.2.8))
Predicted: UniProt sequence A0A127FCT3: RecName: Full=DUF1611 domain-containing protein {ECO:0008006|Google:ProtNLM};
Comment: Bacteroidetes have a divergent N-acylglutamate synthase, see BT3761 (Q8A1A5_BACTN) or Echvi_3845 (L0G3H4_ECHVK). Bacteroides use succinylated intermediates (PMID:16704984), so their proteins are probably N-succinylglutamate synthases. (These enzymes are also known as argA2, see PMC9026213.) Q8P8J6 is annotated as argB in BRENDA, but it is also argA (a fusion protein). N515DRAFT_3768 (A0A1I2DIM7) is similar to ArgAB fusion proteins and mutants are rescued by arginine. It is not clear if mnaT (link) would acetylate arginine, so it is ignored. Some EC 2.3.1.35 enzymes are probably dedicated for converting N-acetylornithine back to ornithine, while others are bifunctional for N-acetylglutamate formation as well (argJ); so similarity to this EC number is inconclusive. For some CharProtDB entries it is unclear if they have this activity or not, while others are labeled with this function but without the EC number. Q9I3W7 is annotated as this in BRENDA but we did not find experimental evidence of its activity on glutamate, so it is ignored. In Steroidobacter denitrificans, argA is missing but the arginine synthesis cluster includes a potential N-acetyltransferase ACG33_RS14135 (A0A127FCT3). Homologs of this protein are conserved in the cluster, and it is never found together with argA; it is distantly related to D-glutamate acetyltransferase dgcN (formerly DUF1611, see PMC10030869).

Fetched 33 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 24 of these sequences

Found 4 clusters of similar sequences. Another 9 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 397-468 amino acids (8/10 heteromeric)

ARGJ_MYCTU / P9WPZ3 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WPZ3 glutamate N-acetyltransferase (EC 2.3.1.35) from Mycobacterium tuberculosis
PFams: ArgJ
Heteromeric, 404 amino acids: PaperBLAST, CDD, Compare to cluster

CH_000559 glutamate N-acetyltransferase/amino-acid acetyltransferase; EC 2.3.1.35 2.3.1.1 from Neisseria gonorrhoeae
PFams: ArgJ
406 amino acids: PaperBLAST, CDD, Compare to cluster

CH_122594 glutamate N-acetyltransferase (Eurofung) from Emericella nidulans
PFams: ArgJ
464 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_SCHPO / O94346 Arginine biosynthesis bifunctional protein ArgJ, mitochondrial; EC 2.3.1.35; EC 2.3.1.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: ArgJ
Heteromeric, 445 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_YEAST / Q04728 Arginine biosynthesis bifunctional protein ArgJ, mitochondrial; Extracellular mutant protein 40; EC 2.3.1.35; EC 2.3.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: ArgJ
Heteromeric, 441 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_GEOSE / Q07908 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Geobacillus stearothermophilus (Bacillus stearothermophilus)
PFams: ArgJ
Heteromeric, 410 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_CITLA / Q3C251 Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 from Citrullus lanatus (Watermelon) (Citrullus vulgaris)
PFams: ArgJ
Heteromeric, 460 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_HALH5 / Q9K8V3 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Halalkalibacterium halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) (Bacillus halodurans)
PFams: ArgJ
Heteromeric, 411 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_THENN / Q9Z4S1 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E)
PFams: ArgJ
Heteromeric, 397 amino acids: PaperBLAST, CDD, Compare to cluster

ARGJ_ARATH / Q9ZUR7 Arginine biosynthesis bifunctional protein ArgJ, chloroplastic; EC 2.3.1.35; EC 2.3.1.1 from Arabidopsis thaliana (Mouse-ear cress)
PFams: ArgJ
Heteromeric, 468 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 368-534 amino acids (not heteromeric)

Q87EL2 amino-acid N-acetyltransferase (EC 2.3.1.1) from Xylella fastidiosa
PFams: AA_kinase, NAT
421 amino acids: PaperBLAST, CDD, Compare to cluster

A0A0H2X8L7 acetylglutamate kinase (EC 2.7.2.8) from Xanthomonas campestris
PFams: AA_kinase, NAT
447 amino acids: PaperBLAST, CDD, Compare to cluster

A4GTP3 amino-acid N-acetyltransferase (EC 2.3.1.1) from Sus scrofa
PFams: AA_kinase, NAT
368 amino acids: PaperBLAST, CDD, Compare to cluster

Q0ASS9 amino-acid N-acetyltransferase (EC 2.3.1.1); acetylglutamate kinase (EC 2.7.2.8) from Maricaulis maris
PFams: AA_kinase, NAT, Acetyltransf_1
441 amino acids: PaperBLAST, CDD, Compare to cluster

NAGS_HUMAN / Q8N159 N-acetylglutamate synthase, mitochondrial; Amino-acid acetyltransferase; EC 2.3.1.1 from Homo sapiens (Human)
Q8N159 amino-acid N-acetyltransferase (EC 2.3.1.1) from Homo sapiens
PFams: AA_kinase, NAT
534 amino acids: PaperBLAST, CDD, Compare to cluster

NAGS_MOUSE / Q8R4H7 N-acetylglutamate synthase, mitochondrial; Amino-acid acetyltransferase; EC 2.3.1.1 from Mus musculus (Mouse)
Q8R4H7 amino-acid N-acetyltransferase (EC 2.3.1.1) from Mus musculus
PFams: NAT
527 amino acids: PaperBLAST, CDD, Compare to cluster

NAGS_DANRE / E7FCP8 N-acetylglutamate synthase, mitochondrial; EC 2.3.1.1 from Danio rerio (Zebrafish) (Brachydanio rerio)
PFams: NAT
527 amino acids: PaperBLAST, CDD, Compare to cluster

N515DRAFT_3768 N-acetylglutamate synthase; N-acetylglutamate kinase (EC 2.3.1.1; EC 2.7.2.8) from Dyella japonica UNC79MFTsu3.2
PFams: AA_kinase, NAT
438 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 192-224 amino acids (not heteromeric)

L0G3H4_ECHVK Acetyltransferase, N-acetylglutamate synthase
224 amino acids: PaperBLAST, CDD, Compare to cluster

Q8A1A5_BACTN GNAT family N-acetyltransferase {ECO:0008006|Google:ProtNLM}
192 amino acids: PaperBLAST, CDD, Compare to cluster

BT3761 N-succinylglutamate synthase from Bacteroides thetaiotaomicron VPI-5482
PFams: Acetyltransf_1
192 amino acids: PaperBLAST, CDD, Compare to cluster

Echvi_3845 N-succinylglutamate synthase from Echinicola vietnamensis KMM 6221, DSM 17526
PFams: Acetyltransf_1
224 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 4 432-443 amino acids (not heteromeric)

P22567 amino-acid N-acetyltransferase (EC 2.3.1.1) from Pseudomonas aeruginosa
PFams: AA_kinase, Acetyltransf_1, Acetyltransf_7
432 amino acids: PaperBLAST, CDD, Compare to cluster

b2818 N-acetylglutamate synthase (EC 2.3.1.1) from Escherichia coli K-12 substr. MG1655
P0A6C5 N-acetylglutamate synthase (EC 2.3.1.1) from Escherichia coli (strain K12)
ARGA_ECOLI / P0A6C5 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Escherichia coli (strain K12)
RF|NP_417295 amino-acid acetyltransferase from Escherichia coli K12
PFams: AA_kinase, Acetyltransf_1, Acetyltransf_7
443 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/9 heteromeric)

C4NFG1 amino-acid N-acetyltransferase (EC 2.3.1.1) from Solanum lycopersicum
PFams: AA_kinase, Acetyltransf_1, Acetyltransf_7
603 amino acids: PaperBLAST, CDD

ARGA_MYCTU / O33289 Amino-acid acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
O33289 amino-acid N-acetyltransferase (EC 2.3.1.1) from Mycobacterium tuberculosis
PFams: Acetyltransf_1, Acetyltransf_7
174 amino acids: PaperBLAST, CDD

Q8NM40 L-glutamate N-acetyltransferase (EC 2.3.1.1) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
Q8NM40 amino-acid N-acetyltransferase (EC 2.3.1.1) from Corynebacterium glutamicum
PFams: Acetyltransf_7, Acetyltransf_1, Acetyltransf_10, FR47
335 amino acids: PaperBLAST, CDD

NAGS_SCHPO / O94330 Amino-acid acetyltransferase, mitochondrial; Arginine-requiring protein 6; Glutamate N-acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: NAT
500 amino acids: PaperBLAST, CDD

NAGS_YEAST / P40360 Amino-acid acetyltransferase, mitochondrial; Arginine-requiring protein 2; Glutamate N-acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: NAT
574 amino acids: PaperBLAST, CDD

NAGS_NEUCR / Q1K8F6 Amino-acid acetyltransferase, mitochondrial; Arginine-requiring protein 14; Glutamate N-acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
PFams: NAT
712 amino acids: PaperBLAST, CDD

NAGS_CANAL / Q59MB6 Amino-acid acetyltransferase, mitochondrial; Arginine-requiring protein 2; Glutamate N-acetyltransferase; N-acetylglutamate synthase; AGS; NAGS; EC 2.3.1.1 from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
PFams: NAT
580 amino acids: PaperBLAST, CDD

A0A127FCT3 DUF1611 domain-containing protein {ECO:0008006|Google:ProtNLM}
357 amino acids: PaperBLAST, CDD

DVU1587 N-acetylglutamate synthase (EC 2.3.1.1) from Desulfovibrio vulgaris Hildenborough JW710
PFams: Acetyltransf_10, Acetyltransf_1, Acetyltransf_7
154 amino acids: PaperBLAST, CDD