Clusters of Characterized Proteins

Clustering the characterized proteins for argX (glutamate--LysW ligase) in L-arginine biosynthesis

Or see other characterized proteins similar to argX

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Definition of argX

• Curated proteins or TIGRFams with EC 6.3.2.60
• Curated sequence Q970U6: Glutamate--LysW ligase ArgX; EC 6.3.2.-
• Ignore hits to items matching 6.3.2.43 when looking for 'other' hits
• Comment: There is also an archaeal pathway from glutamate to ornithine with LysW as the carrier protein, instead of N-acyl intermediates. This pathway is analogous to the conversion of alpha-aminoadipate to lysine, and many of the enzymes are bifunctional. But the initial ligation of LysW to arginine has a dedicated enzyme in many archaea. Q970U6 is given a generic EC number in SwissProt, but has this function. EC 6.4.2.43 (LysW-2-aminoadipate ligases) are ignored because some are bifunctional (PMC5076833).

Fetched 3 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 3 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 273-282 amino acids (not heteromeric)

Q4J8E7 glutamate--LysW ligase monomer (EC 6.3.2.60) from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
ARGX_SULAC / Q4J8E7 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Q4J8E7 glutamate---[amino group carrier protein] ligase (EC 6.3.2.60) from Sulfolobus acidocaldarius
PFams: ATP-grasp_3, RimK, GSH-S_ATP
282 amino acids: PaperBLAST, CDD, Compare to cluster

Q5JFW0 lysyltransferase (EC 2.3.2.3); [amino group carrier protein]-L-2-aminoadipate ligase (EC 6.3.2.43); glutamate---[amino group carrier protein] ligase (EC 6.3.2.60) from Thermococcus kodakarensis
PFams: RimK
273 amino acids: PaperBLAST, CDD, Compare to cluster

ARGX_SULTO / Q970U6 Glutamate--LysW ligase ArgX; EC 6.3.2.- from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii)
PFams: ATP-grasp_3, RimK, GSH-S_ATP
282 amino acids: PaperBLAST, CDD, Compare to cluster