Clusters of Characterized Proteins

Clustering the characterized proteins for asnA (aspartate--ammonia ligase) in L-asparagine biosynthesis

Or see other characterized proteins similar to asnA

Or see all steps for L-asparagine biosynthesis

Or cluster curated proteins matching a keyword

Definition of asnA

• Curated proteins or TIGRFams with EC 6.3.1.1
• Curated sequence A4I213: asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4)
• Ignore hits to items matching EC 6.3.5.4 when looking for 'other' hits
• Ignore hits to CH_123066 when looking for 'other' hits (asparagine synthetase)
• Comment: In BRENDA, A4I213 (asnA) is misannotated as asnB. Many asparagine synthases are bifunctional (obtaining nitrogen from either ammonia or glutamine) so similarity to EC 6.3.5.4 is ignored. CH_123066 has a vague annotation and it is not clear if it is actually characterized.

Fetched 5 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 3 of these sequences

Found 1 clusters of similar sequences. Another 2 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 330-353 amino acids (not heteromeric)

A4I213 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Leishmania infantum
PFams: AsnA
353 amino acids: PaperBLAST, CDD, Compare to cluster

Q57WT9 aspartate-ammonia ligase (EC 6.3.1.1) from Trypanosoma brucei brucei
PFams: AsnA
351 amino acids: PaperBLAST, CDD, Compare to cluster

b3744 asparagine synthetase A (EC 6.3.1.1) from Escherichia coli K-12 substr. MG1655
P00963 asparagine synthetase A (EC 6.3.1.1) from Escherichia coli (strain K12)
ASNA_ECOLI / P00963 Aspartate--ammonia ligase; Asparagine synthetase A; EC 6.3.1.1 from Escherichia coli (strain K12)
PFams: AsnA
330 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/2 heteromeric)

Q9V228 asparagine-tRNA ligase (EC 6.1.1.22); aspartate-ammonia ligase (EC 6.3.1.1) from Pyrococcus abyssi
PFams: tRNA-synt_2
294 amino acids: PaperBLAST, CDD

b0674 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38; EC 3.5.1.2) from Escherichia coli K-12 substr. MG1655
P22106 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38) from Escherichia coli (strain K12)
ASNB_ECOLI / P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12)
CH_002444 asparagine synthetase B, glutamine-hydrolyzing; EC 6.3.5.4 from Escherichia coli
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
554 amino acids: PaperBLAST, CDD