Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for asnB (asparagine synthase (glutamine-hydrolysing)) in L-asparagine biosynthesis

Or see other characterized proteins similar to asnB

Or see all steps for L-asparagine biosynthesis

Or cluster curated proteins matching a keyword

Definition of asnB

Curated proteins or TIGRFams with EC 6.3.5.4
Ignore hits to A4I213 when looking for 'other' hits (asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4))
Ignore hits to items matching EC 6.3.1.1 when looking for 'other' hits
Ignore hits to CH_123066 when looking for 'other' hits (asparagine synthetase)
Comment: In BRENDA, A4I213 is misannotated as asnB. Many asparagine synthases are bifunctional (obtaining nitrogen from either ammonia or glutamine) so similarity to EC 6.3.1.1 is ignored. CH_123066 has a vague annotation and it is not clear if it is actually characterized.

Fetched 38 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 38 of these sequences

Found 3 clusters of similar sequences. Another 1 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 475-604 amino acids (not heteromeric)

A0A1D5VHV8 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Triticum aestivum
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
581 amino acids: PaperBLAST, CDD, Compare to cluster

A0A1D5YL35 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Triticum aestivum
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
585 amino acids: PaperBLAST, CDD, Compare to cluster

A9XS73 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
584 amino acids: PaperBLAST, CDD, Compare to cluster

B3KYI2 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
570 amino acids: PaperBLAST, CDD, Compare to cluster

D9IXC8 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus pinaster
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
590 amino acids: PaperBLAST, CDD, Compare to cluster

E7EAQ2 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus pinaster
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase
593 amino acids: PaperBLAST, CDD, Compare to cluster

F2CQ60 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
591 amino acids: PaperBLAST, CDD, Compare to cluster

F2CWZ7 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
581 amino acids: PaperBLAST, CDD, Compare to cluster

F2DDF6 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
475 amino acids: PaperBLAST, CDD, Compare to cluster

F2DJ31 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
589 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS_HUMAN / P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human)
P08243 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Homo sapiens
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
561 amino acids: PaperBLAST, CDD, Compare to cluster

Q10MX3 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Oryza sativa Japonica Group
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
604 amino acids: PaperBLAST, CDD, Compare to cluster

Q14SM9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Glycine max
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
569 amino acids: PaperBLAST, CDD, Compare to cluster

Q2L9S5 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Bombyx mori
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
551 amino acids: PaperBLAST, CDD, Compare to cluster

Q6HA26 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Pinus sylvestris
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase
593 amino acids: PaperBLAST, CDD, Compare to cluster

Q84LA5 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
581 amino acids: PaperBLAST, CDD, Compare to cluster

Q93XP9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Hordeum vulgare subsp. vulgare
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
585 amino acids: PaperBLAST, CDD, Compare to cluster

Q9LKQ9 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
589 amino acids: PaperBLAST, CDD, Compare to cluster

Q9SM55 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Phaseolus vulgaris
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
579 amino acids: PaperBLAST, CDD, Compare to cluster

Q9SP19 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
558 amino acids: PaperBLAST, CDD, Compare to cluster

Q9SP20 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Helianthus annuus
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
591 amino acids: PaperBLAST, CDD, Compare to cluster

b0674 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38; EC 3.5.1.2) from Escherichia coli K-12 substr. MG1655
P22106 asparagine synthetase B (EC 6.3.5.4; EC 6.3.1.1; EC 3.5.1.38) from Escherichia coli (strain K12)
ASNB_ECOLI / P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12)
CH_002444 asparagine synthetase B, glutamine-hydrolyzing; EC 6.3.5.4 from Escherichia coli
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
554 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS_SCHPO / P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
RF|NP_595291.1 asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
557 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS1_ARATH / P49078 Asparagine synthetase [glutamine-hydrolyzing] 1; Glutamine-dependent asparagine synthetase 1; Protein DARK INDUCIBLE 6; EC 6.3.5.4 from Arabidopsis thaliana (Mouse-ear cress)
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
584 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS2_YEAST / P49090 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
572 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS2_ORYSJ / Q43011 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Oryza sativa subsp. japonica (Rice)
Q43011 asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4) from Oryza sativa Japonica Group
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
591 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS_MOUSE / Q61024 Asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Mus musculus (Mouse)
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
561 amino acids: PaperBLAST, CDD, Compare to cluster

ASNS2_ARATH / Q9LV77 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Arabidopsis thaliana (Mouse-ear cress)
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
578 amino acids: PaperBLAST, CDD, Compare to cluster

P49088 asparagine synthetase subunit (EC 6.3.5.4) from Rattus norvegicus
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
561 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 591-652 amino acids (not heteromeric)

GB|CAB15032.1 asparagine synthase (glutamine-hydrolysing) 1; EC 6.3.5.4 from Bacillus subtilis
PFams: GATase_6, GATase_7, Asn_synthase
632 amino acids: PaperBLAST, CDD, Compare to cluster

GI|1934835 asparagine synthase (glutamine-hydrolysing) 3; EC 6.3.5.4 from Bacillus subtilis
PFams: GATase_6, GATase_7, Asn_synthase
614 amino acids: PaperBLAST, CDD, Compare to cluster

ASNH_MYCBO / P64248 Putative asparagine synthetase [glutamine-hydrolyzing]; EC 6.3.5.4 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
PFams: GATase_6, GATase_7, Asn_synthase
652 amino acids: PaperBLAST, CDD, Compare to cluster

Q9CDJ2 peptidoglycan asparagine synthase (from HMM only) from Lactococcus lactis subsp. lactis (strain IL1403)
PFams: GATase_6, GATase_7, Asn_synthase
625 amino acids: PaperBLAST, CDD, Compare to cluster

Q92W82 N-acetylglutaminylglutamine amide synthase (from HMM only) from Rhizobium meliloti (strain 1021)
PFams: GATase_6, GATase_7, Asn_synthase
591 amino acids: PaperBLAST, CDD, Compare to cluster

E2JA33 N-3-fumaroyl-(S)-2,3-diaminopropanoate aminotransferase (from HMM only) from Enterobacter agglomerans
PFams: GATase_6, GATase_7, NAD_synthase, Asn_synthase
607 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 671-672 amino acids (not heteromeric)

CHYD_PENRW / B6HLP8 Amidase chyE; Chrysogine biosynthesis cluster protein D; EC 3.-.-.- (from HMM only) from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
PFams: GATase_6, GATase_7, Asn_synthase
672 amino acids: PaperBLAST, CDD, Compare to cluster

CHRY2_GIBZE / I1S3K8 Amidase chry2; Chrysogine biosynthesis cluster protein 2; EC 3.-.-.- (from HMM only) from Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
PFams: GATase_6, GATase_7, Asn_synthase
671 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/1 heteromeric)

L8EVF2 malonamoyl-CoA synthetase (from HMM only) from Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234)
PFams: GATase_6, GATase_7, Asn_synthase
612 amino acids: PaperBLAST, CDD
(similar to CharProtDB::CH_007942 from Cluster 2, but similarity to seed sequence is below thresholds)