Clusters of Characterized Proteins

Clustering the characterized proteins for aatJ (aspartate/asparagine ABC transporter, substrate-binding component AatJ) in L-asparagine catabolism

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Definition of aatJ

• Curated sequence Q88NY2: PP1071, component of Acidic amino acid uptake porter, AatJMQP
• Curated sequence AO353_16290: ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, periplasmic substrate-binding component
• Curated sequence Pf1N1B4_771: ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component
• Curated sequence PfGW456L13_4770: ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component
• Ignore hits to Q9I402 when looking for 'other' hits (Probable binding protein component of ABC transporter, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida)
• Curated sequence CH_002441: glutamate-aspartate periplasmic-binding protein. Glutamate/aspartate import solute-binding protein. YBEJ aka GltI aka B0655, component of Glutamate/aspartate porter. glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1). glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1)
• Comment: AatJMQP from Pseudomonas putida KT2440, TC 3.A.1.3.19, is quite similar to an aspartate ABC transporter from P. fluorescens N2E3 and to GltIJKL from E. coli. It is also very similar to asparagine/glutamate transporters from other Pseudomonas, which might transport aspartate as well. (aatJ = PP1071 or AO353_16290 or gltI)

Fetched 5 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 5 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 302-306 amino acids (heteromeric)

b0655 glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli K-12 substr. MG1655
P37902 glutamate/aspartate ABC transporter periplasmic binding protein (EC 7.4.2.1) from Escherichia coli (strain K12)
GLTI_ECOLI / P37902 Glutamate/aspartate import solute-binding protein from Escherichia coli (strain K12)
TC 3.A.1.3.4 / P37902 YBEJ aka GltI aka B0655, component of Glutamate/aspartate porter from Escherichia coli
GB|BAA35307.2 glutamate-aspartate periplasmic-binding protein from Escherichia coli K12
PFams: SBP_bac_3
Heteromeric, 302 amino acids: PaperBLAST, CDD, Compare to cluster

TC 3.A.1.3.19 / Q88NY2 PP1071, component of Acidic amino acid uptake porter, AatJMQP from Pseudomonas putida (strain KT2440)
PFams: SBP_bac_3
Heteromeric, 306 amino acids: PaperBLAST, CDD, Compare to cluster

PfGW456L13_4770 ABC transporter for L-Asparagine and possibly other L-amino acids, periplasmic substrate-binding component from Pseudomonas fluorescens GW456-L13
PFams: SBP_bac_3
Heteromeric, 304 amino acids: PaperBLAST, CDD, Compare to cluster

Pf1N1B4_771 ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N1B4
PFams: SBP_bac_3, NMT1
Heteromeric, 304 amino acids: PaperBLAST, CDD, Compare to cluster

AO353_16290 ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, periplasmic substrate-binding component from Pseudomonas fluorescens FW300-N2E3
PFams: SBP_bac_3, NMT1
Heteromeric, 304 amino acids: PaperBLAST, CDD, Compare to cluster