Clusters of Characterized Proteins

Clustering the characterized proteins for mcl ((S)-citramalyl-CoA pyruvate-lyase) in L-glutamate catabolism

Or see other characterized proteins similar to mcl

Or see all steps for L-glutamate catabolism

Or cluster curated proteins matching a keyword

Definition of mcl

• Curated proteins or TIGRFams with EC 4.1.3.25
• Ignore hits to GFF3000 when looking for 'other' hits (Malyl-CoA lyase (EC 4.1.3.24))
• Ignore hits to B6E2X2 when looking for 'other' hits (L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; EC 4.1.3.24. malyl-CoA lyase subunit (EC 4.1.3.24))
• Comment: Although the citramalate lyase is described as a single reaction in MetaCyc, the enzyme database entry (EC 4.1.3.22) suggests that it is a two-step reaction: citramalate CoA-transferase (EC 2.8.3.11) and citramalyl-CoA lyase (EC 4.1.3.25). No citramalate CoA-transferase proteins have been linked to sequence, but citramalyl-CoA lyases are known, so only this step is included. Some (S-citramalyl-CoA lyases are very similar to malyl-CoA lyases, which are marked ignore.

Fetched 6 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 6 of these sequences

Found 2 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 275-340 amino acids (not heteromeric)

A0A172MLA1 (S)-citramalyl-CoA lyase (EC 4.1.3.25); (R)-citramalyl-CoA lyase (EC 4.1.3.46) from Aspergillus terreus
PFams: HpcH_HpaI
322 amino acids: PaperBLAST, CDD, Compare to cluster

Q8N0X4 malate/β-methylmalate synthase (EC 2.3.3.9) from Homo sapiens
CLYBL_HUMAN / Q8N0X4 Citramalyl-CoA lyase, mitochondrial; (3S)-malyl-CoA thioesterase; Beta-methylmalate synthase; Citrate lyase subunit beta-like protein; Citrate lyase beta-like; Malate synthase; EC 4.1.3.25; EC 3.1.2.30; EC 2.3.3.-; EC 2.3.3.9 from Homo sapiens (Human)
Q8N0X4 citrate (pro-3S)-lyase (EC 4.1.3.6) from Homo sapiens
PFams: HpcH_HpaI
340 amino acids: PaperBLAST, CDD, Compare to cluster

Q9I562 (S)-citramalyl-CoA lyase (EC 4.1.3.25) from Pseudomonas aeruginosa
PFams: HpcH_HpaI
275 amino acids: PaperBLAST, CDD, Compare to cluster

CLYBL_MOUSE / Q8R4N0 Citramalyl-CoA lyase, mitochondrial; (3S)-malyl-CoA thioesterase; Beta-methylmalate synthase; Citrate lyase subunit beta-like protein, mitochondrial; Citrate lyase beta-like; Malate synthase; EC 4.1.3.25; EC 3.1.2.30; EC 2.3.3.-; EC 2.3.3.9 from Mus musculus (Mouse)
Q8R4N0 citrate (pro-3S)-lyase (EC 4.1.3.6) from Mus musculus
PFams: HpcH_HpaI, C-C_Bond_Lyase
338 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 318-348 amino acids (not heteromeric)

MCAL_RHOS4 / Q3J5L6 L-malyl-CoA/beta-methylmalyl-CoA lyase; (3S)-malyl-CoA/beta-methylmalyl-CoA lyase; (S)-citramalyl-CoA lyase; EC 4.1.3.24; EC 4.1.3.25 from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
Q3J5L6 malyl-CoA lyase (EC 4.1.3.24) from Rhodobacter sphaeroides
PFams: HpcH_HpaI
318 amino acids: PaperBLAST, CDD, Compare to cluster

A9WC35 L-malyl CoA lyase subunit (EC 4.1.3.25; EC 4.1.3.24) from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)
MCLA_CHLAU / S5N020 Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase; (3S)-3-carboxy-3-hydroxypropanoyl-CoA glyoxylate-lyase; (3S)-citramalyl-CoA pyruvate-lyase; (S)-citramalyl-CoA lyase; Erythro-beta-methylmalyl-CoA; L-malyl-CoA lyase; EC 4.1.3.24; EC 4.1.3.25 from Chloroflexus aurantiacus
S5N020 malyl-CoA lyase (EC 4.1.3.24); (S)-citramalyl-CoA lyase (EC 4.1.3.25) from Chloroflexus aurantiacus
PFams: HpcH_HpaI
348 amino acids: PaperBLAST, CDD, Compare to cluster