Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for hisN (histidinol-phosphate phosphatase) in L-histidine biosynthesis

Or see other characterized proteins similar to hisN

Or see all steps for L-histidine biosynthesis

Or cluster curated proteins matching a keyword

Definition of hisN

Curated proteins or TIGRFams with EC 3.1.3.15
UniProt sequence B9E9Z0_MACCJ: RecName: Full=Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003};
UniProt sequence C0ZH63_BREBN: RecName: Full=Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003};
UniProt sequence Q73B87_BACC1: RecName: Full=Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003};
UniProt sequence A9VLI0_BACMK: RecName: Full=Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003};
UniProt sequence HIS9_BACHD: RecName: Full=Histidinol-phosphatase; Short=HolPase; EC=3.1.3.15;
UniProt sequence Q5KW52_GEOKA: RecName: Full=Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003};
UniProt sequence Q8DT80_STRMU: RecName: Full=Histidinol-phosphatase {ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|RuleBase:RU366003};
UniProt sequence HIS9_BACSU: RecName: Full=Histidinol-phosphatase; Short=HolPase; EC=3.1.3.15;
UniProt sequence A0A0L7BRC5: SubName: Full=HAD family hydrolase {ECO:0000313|EMBL:KOA49733.1};
Ignore hits to S5FT07 when looking for 'other' hits (Carbon disulfide hydrolase; CS(2) hydrolase; EC 3.13.1.5. carbon disulfide hydrolase (EC 3.13.1.5))
Ignore hits to S5FU55 when looking for 'other' hits (Carbon disulfide hydrolase; CS(2) hydrolase; EC 3.13.1.5. carbon disulfide hydrolase (EC 3.13.1.5))
Ignore hits to ALKAPHOSPHA-MONOMER when looking for 'other' hits (alkaline phosphatase (EC 3.1.3.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.108; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.108; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1))
UniProt sequence D4GRX2: SubName: Full=HAD superfamily hydrolase {ECO:0000313|EMBL:ADE03629.1};
UniProt sequence A0A1I1YPH6: SubName: Full=HAD-superfamily subfamily IB hydrolase, TIGR01490 {ECO:0000313|EMBL:SFE20043.1};
UniProt sequence A9A5X4: SubName: Full=Haloacid dehalogenase domain protein hydrolase {ECO:0000313|EMBL:ABX13452.1};
Comment: In Bacillus subtilis and some related bacteria, histidinol-phosphate phosphatase is known as HisJ and has been confirmed by biochemical assays of purified proteins (PMC3570733). The identifiers given (see their Table 3) are MCCL_0344 BBR47_00270 BCE_1533 BcerKBAB4_1335 BcerKBAB4_1335 BSU29620 BH3206 GK2799 SMU_1486c (B9E9Z0_MACCJ C0ZH63_BREBN Q73B87_BACC1 A9VLI0_BACMK HIS9_BACHD Q5KW52_GEOKA Q8DT80_STRMU HIS9_BACSU). In Bifidobacterium breve, the phosphatase activity is provided by Bbr_0982 (A0A0L7BRC5, link). S5FT07 and S5FU55 are misannotated as this in BRENDA. E. coli phoA (link) has this activity but is ignored because it is periplasmic. HVO_0431 (D4GRX2) from Haloferax has auxotrophic phenotypes and must be the missing hisN (PMC4300041; PMC8305020). A0A1I1YPH6 ("Beta1") and Nmar_1556 (A9A5X4) were shown biochemically to be hisN (PMC10804674); Beta1 is similar to BPHYT_RS03625, which has auxotrophic phenotypes; Nmar_1556 lies in a histidine synthesis operon and must be the missing hisN.

Fetched 34 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 27 of these sequences

Found 5 clusters of similar sequences. Another 8 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 250-326 amino acids (not heteromeric)

PGA1_c21860 Histidinol-phosphatase [alternative form] (EC 3.1.3.15) from Phaeobacter inhibens BS107
PFams: Inositol_P
250 amino acids: PaperBLAST, CDD, Compare to cluster

G7J7Q5 histidinol-phosphatase (EC 3.1.3.15); inositol-phosphate phosphatase (EC 3.1.3.25) from Medicago truncatula
PFams: Inositol_P
326 amino acids: PaperBLAST, CDD, Compare to cluster

HISN_MYCTU / P95189 Histidinol-phosphatase; HolPase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P95189 histidinol-phosphatase (EC 3.1.3.15) from Mycobacterium tuberculosis
PFams: Inositol_P
260 amino acids: PaperBLAST, CDD, Compare to cluster

Q8NS79 histidinol-phosphatase (EC 3.1.3.15) from Corynebacterium glutamicum
PFams: Inositol_P
273 amino acids: PaperBLAST, CDD, Compare to cluster

Q8NS80 histidinol-phosphatase (EC 3.1.3.15) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
HISN_CORGL / Q8NS80 Histidinol-phosphatase; HolPase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
Q8NS80 histidinol-phosphatase (EC 3.1.3.15) from Corynebacterium glutamicum
PFams: Inositol_P
260 amino acids: PaperBLAST, CDD, Compare to cluster

HISN_STRCO / Q9K4B1 Histidinol-phosphatase; HolPase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
PFams: Inositol_P
266 amino acids: PaperBLAST, CDD, Compare to cluster

Ga0059261_2035 Histidinol-phosphatase [alternative form] (EC 3.1.3.15) from Sphingomonas koreensis DSMZ 15582
PFams: Inositol_P
260 amino acids: PaperBLAST, CDD, Compare to cluster

AZOBR_RS03845 Histidinol-phosphatase [alternative form] (EC 3.1.3.15) from Azospirillum brasilense Sp245
PFams: Inositol_P
260 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 217-234 amino acids (not heteromeric)

HIS9_NEIM8 / P0DV34 Histidinol-phosphatase; Hol-Pase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Neisseria meningitidis serogroup C (strain 8013)
PFams: Hydrolase, HAD
222 amino acids: PaperBLAST, CDD, Compare to cluster

HIS9_PSEAE / Q9I6F6 Histidinol-phosphatase; Hol-Pase; Histidinol-phosphate phosphatase; EC 3.1.3.15 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PFams: Hydrolase, HAD
217 amino acids: PaperBLAST, CDD, Compare to cluster

A0A1I1YPH6 HAD-superfamily subfamily IB hydrolase, TIGR01490
234 amino acids: PaperBLAST, CDD, Compare to cluster

BPHYT_RS03625 Histidinol-phosphatase (EC:3.1.3.15) from Burkholderia phytofirmans PsJN
PFams: HAD
228 amino acids: PaperBLAST, CDD, Compare to cluster

HP15_461 Histidinol-phosphatase (EC:3.1.3.15) from Marinobacter adhaerens HP15
PFams: Hydrolase, HAD
218 amino acids: PaperBLAST, CDD, Compare to cluster

Psest_3864 Histidinol-phosphatase (EC:3.1.3.15) from Pseudomonas stutzeri RCH2
PFams: HAD
217 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 267-339 amino acids (not heteromeric)

C0ZH63_BREBN Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}
334 amino acids: PaperBLAST, CDD, Compare to cluster

A9VLI0_BACMK Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}
339 amino acids: PaperBLAST, CDD, Compare to cluster

B9E9Z0_MACCJ Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}
335 amino acids: PaperBLAST, CDD, Compare to cluster

Q73B87_BACC1 Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}
338 amino acids: PaperBLAST, CDD, Compare to cluster

Q5SLG2 histidinol phosphate phosphatase subunit (EC 3.1.3.15) from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
PFams: PHP, PHP_C
267 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 4 355-382 amino acids (not heteromeric)

HIS7_SPILD / D2QPE6 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Spirosoma linguale (strain ATCC 33905 / DSM 74 / LMG 10896 / Claus 1)
PFams: PNK3P, Hydrolase_like, IGPD
382 amino acids: PaperBLAST, CDD, Compare to cluster

HIS7_BACTN / Q8ABA7 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
PFams: Hydrolase_like, IGPD
374 amino acids: PaperBLAST, CDD, Compare to cluster

HIS7_ECO57 / Q9S5G5 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Escherichia coli O157:H7
PFams: PNK3P, Hydrolase_like, IGPD
355 amino acids: PaperBLAST, CDD, Compare to cluster

b2022 imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (EC 4.2.1.19; EC 3.1.3.15) from Escherichia coli K-12 substr. MG1655
P06987 imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (EC 4.2.1.19; EC 3.1.3.15) from Escherichia coli (strain K12)
PFams: PNK3P, Hydrolase_like, IGPD
355 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 5 268-274 amino acids (not heteromeric)

HIS9_BACHD Histidinol-phosphatase; Short=HolPase; EC=3.1.3.15
273 amino acids: PaperBLAST, CDD, Compare to cluster

HIS9_BACSU Histidinol-phosphatase; Short=HolPase; EC=3.1.3.15
268 amino acids: PaperBLAST, CDD, Compare to cluster

Q5KW52_GEOKA Histidinol-phosphatase {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|ARBA:ARBA00013085, ECO:0000256|RuleBase:RU366003}
274 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/8 heteromeric)

B1PRL6 histidinol-phosphatase (EC 3.1.3.15) from Thermococcus onnurineus NA1
240 amino acids: PaperBLAST, CDD

Q02150 histidinol-phosphatase (EC 3.1.3.15) from Lactococcus lactis subsp. lactis
PFams: PHP
269 amino acids: PaperBLAST, CDD

Q6NPM8 histidinol phosphate phosphatase (EC 3.1.3.15; EC 3.1.3.93; EC 3.1.3.25) from Arabidopsis thaliana
HIS7_ARATH / Q6NPM8 Bifunctional phosphatase IMPL2, chloroplastic; Histidinol-phosphatase; Histidinol-phosphate phosphatase; HPP; Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Protein HISTIDINE BIOSYNTHESIS 7; Protein MYO-INOSITOL MONOPHOSPHATASE-LIKE 2; EC 3.1.3.15; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress)
Q6NPM8 histidinol-phosphatase (EC 3.1.3.15); inositol-phosphate phosphatase (EC 3.1.3.25) from Arabidopsis thaliana
PFams: Inositol_P
346 amino acids: PaperBLAST, CDD
(similar to BRENDA::G7J7Q5 from Cluster 1, but similarity to seed sequence is below thresholds)

HIS9_YEAST / P38635 Histidinol-phosphatase; HolPase; EC 3.1.3.15 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: PHP
335 amino acids: PaperBLAST, CDD

A0A0L7BRC5 HAD family hydrolase
209 amino acids: PaperBLAST, CDD

A9A5X4 Haloacid dehalogenase domain protein hydrolase
306 amino acids: PaperBLAST, CDD

D4GRX2 HAD superfamily hydrolase
284 amino acids: PaperBLAST, CDD

Q8DT80_STRMU Histidinol-phosphatase {ECO:0000256|RuleBase:RU366003}; Short=HolPase {ECO:0000256|RuleBase:RU366003}; EC=3.1.3.15 {ECO:0000256|RuleBase:RU366003}
244 amino acids: PaperBLAST, CDD