Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for cimA ((R)-citramalate synthase) in L-isoleucine biosynthesis

Or see other characterized proteins similar to cimA

Or see all steps for L-isoleucine biosynthesis

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Definition of cimA

Curated proteins or TIGRFams with EC 2.3.1.182
Curated proteins or TIGRFams with EC 2.3.3.21
UniProt sequence Q8F3Q1: RecName: Full=(R)-citramalate synthase CimA {ECO:0000305}; EC=2.3.3.21 {ECO:0000269|PubMed:15292141, ECO:0000269|PubMed:18498255, ECO:0000269|PubMed:19351325}; AltName: Full=LiCMS {ECO:0000303|PubMed:18498255};
UniProt sequence D4GSQ2: SubName: Full=2-isopropylmalate synthase / (R)-citramalate synthase {ECO:0000313|EMBL:ADE04954.1}; EC=2.3.1.182 {ECO:0000313|EMBL:ADE04954.1}; EC=2.3.3.13 {ECO:0000313|EMBL:ADE04954.1};
Comment: MetaCyc L-isoleucine biosynthesis II describes the formation of 2-oxobutanoate via citramalate. The other steps are the same (although it gives a different EC number for ilvC because of different cofactor preference) The citramalate synthase from Leptopsira interrogans (LA_2350, NP_712531, or Q8F3Q1) has been characterized biochemically but is not in the curated databases, see PMID:18498255 The putative citramalate synthase HVO_0644 (D4GSQ2) from Haloferax volcanii is required for isoleucine biosynthesis, see PMC4300041.

Fetched 9 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 9 of these sequences

Found 2 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 491-523 amino acids (not heteromeric)

Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii
PFams: HMGL-like, LeuA_dimer
491 amino acids: PaperBLAST, CDD, Compare to cluster

B0K6M2 (R)-citramalate synthase (EC 2.3.3.21) from Thermoanaerobacter sp.
PFams: HMGL-like, LeuA_dimer
520 amino acids: PaperBLAST, CDD, Compare to cluster

CIMA_SULAC / Q4J6H1 (R)-citramalate synthase; Citramalate synthase; CMS; EC 2.3.3.21 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
PFams: HMGL-like, LeuA_dimer
523 amino acids: PaperBLAST, CDD, Compare to cluster

Q8F3Q1 (R)-citramalate synthase subunit (EC 2.3.3.21) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
CIMA_LEPIN / Q8F3Q1 (R)-citramalate synthase CimA; LiCMS; EC 2.3.3.21 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
PFams: HMGL-like, LeuA_dimer
516 amino acids: PaperBLAST, CDD, Compare to cluster

D4GSQ2 2-isopropylmalate synthase / (R)-citramalate synthase; EC=2.3.1.182; EC=2.3.3.13
512 amino acids: PaperBLAST, CDD, Compare to cluster

Q8F3Q1 (R)-citramalate synthase CimA; EC=2.3.3.21 {ECO:0000269|PubMed:15292141, ECO:0000269|PubMed:18498255, ECO:0000269|PubMed:19351325}; LiCMS {ECO:0000303|PubMed:18498255}
516 amino acids: PaperBLAST, CDD, Compare to cluster

BT1858 (R)-citramalate synthase (EC 2.3.1.182) from Bacteroides thetaiotaomicron VPI-5482
PFams: HMGL-like, LeuA_dimer
499 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 528-538 amino acids (not heteromeric)

Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
CIMA_GEOSL / Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.3.21 from Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Q74C76 (R)-citramalate synthase (EC 2.3.3.21) from Geobacter sulfurreducens
PFams: HMGL-like, LeuA_dimer
528 amino acids: PaperBLAST, CDD, Compare to cluster

DVU1914 (R)-citramalate synthase (EC 2.3.3.21) from Desulfovibrio vulgaris Hildenborough JW710
PFams: HMGL-like, LeuA_dimer
538 amino acids: PaperBLAST, CDD, Compare to cluster