Clusters of Characterized Proteins

Clustering the characterized proteins for ilvH (acetolactate/acetohydroxybutanoate synthase regulatory subunit ) in L-isoleucine biosynthesis

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Definition of ilvH

• HMM TIGR00119
• Curated proteins matching acetohydroxy-acid synthase%small
• Curated proteins matching acetohydroxybutanoate synthase, regulatory subunit
• Curated proteins matching small subunit of acetolactate synthase
• Curated sequence P0ADG1: acetolactate synthase (subunit 1/2) (EC 2.2.1.6). acetolactate synthase II subunit IlvM (EC 2.2.1.6)
• Ignore hits to items matching EC 2.2.1.6 when looking for 'other' hits
• UniProt sequence A0A154R0Y7: RecName: Full=Acetolactate synthase small subunit {ECO:0000256|RuleBase:RU368092}; Short=AHAS {ECO:0000256|RuleBase:RU368092}; Short=ALS {ECO:0000256|RuleBase:RU368092}; EC=2.2.1.6 {ECO:0000256|RuleBase:RU368092}; AltName: Full=Acetohydroxy-acid synthase small subunit {ECO:0000256|RuleBase:RU368092};
• Curated sequence Q93YZ7: Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1
• UniProt sequence A0A0H2X4P1: SubName: Full=Acetolactate synthase isozyme II small subunit {ECO:0000313|EMBL:AAY47914.1};
• Predicted: UniProt sequence A0A7J3AYJ4: SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:HGD33185.1};
• Predicted: UniProt sequence A0A161J739: SubName: Full=Acetolactate synthase 3 regulatory subunit domain protein {ECO:0000313|EMBL:ANC53960.1};
• Comment: The isolated catalytic subunit can have some activity on its own, so it's not clear if the regulatory subunit (ilvH) is always required, but ilvH does always seem to be present. P0ADG1 is annotated with this EC number but not explicitly as the small regulatory subunit, so it was added manually. Q93YZ7 is annotated as this but without the EC number, so is added manually. Most regulatory subunits have an N-terminal ACT domain and a C-terminal ACT-like domain, but E. coli IlvM, which is required for the activity of E. coli acetohydroxyacid synthase isoenzyme II, has the N-terminal ACT domain only. We identified several other short (one-domain) regulatory subunits. In Rhodanobacter and related genera, the putative regulatory subunit has just one ACT domain (i.e., LRK54_RS10305, which is nearly identical to A0A154R0Y7). Based on sequence analysis, short ilvH probably maintains the ability to bind valine and to bind the catalytic subunit, but not the ability to bind ATP or other regulatory subunits. Mutant fitness data confirms that LRK54_RS10305 is involved in amino acid biosynthesis. In Xanthomonas campestris, the one ACT-domain protein Xcc-8004.1058.1 (A0A0H2X4P1), which is conserved next to ilvI, has a similar fitness pattern as ilvI (Alice Castaing, unpublished data). Furthermore, its AlphaFold structure is very similar to that of E. coli IlvM (TM-score 0.89, RMSD 1.49 A, foldseek). So Xcc-8004.1058.1 is another short regulatory subunit. Similarly, in Brevundimonas sp. GW460-12-10-14-LB2, the putative ilvH has the ACT domain only (Brev2_1981 = A0A161J739). Many Thermoproteota seem to have a diverged short regulatory subunit, such as the ACT domain protein KCR_RS03285 (A0A7J3AYJ4), which is conserved next to ilvI. Foldseek shows that this protein is similar to the ACT domain of (p)ppGpp synthase but also to IlvH of Staphylococcus aureus, so we predict that it is the regulatory subunit.

Fetched 14 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 12 of these sequences

Found 4 clusters of similar sequences. Another 2 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 289-326 amino acids (not heteromeric)

CH_122620 small subunit of acetolactate synthase from Emericella nidulans
PFams: ACT, ACT_5, ALS_ss_C
326 amino acids: PaperBLAST, CDD, Compare to cluster

ILV6_SCHPO / O60086 Probable acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: ACT, ACT_5, ALS_ss_C
289 amino acids: PaperBLAST, CDD, Compare to cluster

ILV6_YEAST / P25605 Acetolactate synthase small subunit, mitochondrial; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: ACT, ACT_5, ALS_ss_C
309 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 449-491 amino acids (not heteromeric)

ILVH2_ARATH / Q9FFF4 Acetolactate synthase small subunit 2, chloroplastic; ALS-interacting protein 3; Acetohydroxy-acid synthase small subunit 2; Protein valine-tolerant 1 from Arabidopsis thaliana (Mouse-ear cress)
PFams: ACT, ACT_5, ALS_ss_C, ACT, ACT_5, ALS_ss_C
477 amino acids: PaperBLAST, CDD, Compare to cluster

ILVH1_ARATH / Q93YZ7 Acetolactate synthase small subunit 1, chloroplastic; ALS-interacting protein 1; Acetohydroxyacid synthase small subunit 1 from Arabidopsis thaliana (Mouse-ear cress)
PFams: ACT, ACT_5, ALS_ss_C, ACT, ACT_5, ALS_ss_C
491 amino acids: PaperBLAST, CDD, Compare to cluster

ILVH_NICPL / Q9SMC2 Acetolactate synthase small subunit 1, chloroplastic; Acetohydroxy-acid synthase small subunit; AHAS; ALS from Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
PFams: ACT_5, ACT, ALS_ss_C, ACT, ACT_5, ALS_ss_C
449 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 163-169 amino acids (heteromeric)

b0078 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655
P00894 acetolactate synthase / acetohydroxybutanoate synthase, regulatory subunit (EC 2.2.1.6) from Escherichia coli (strain K12)
P00894 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Escherichia coli
GB|BAB96647.2 acetolactate synthase, small subunit; EC 2.2.1.6 from Escherichia coli
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 163 amino acids: PaperBLAST, CDD, Compare to cluster

ILVH_MYCTU / P9WKJ3 Putative acetolactate synthase small subunit; Acetohydroxy-acid synthase small subunit; AHAS; ALS; EC 2.2.1.6 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WKJ3 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Mycobacterium tuberculosis
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 168 amino acids: PaperBLAST, CDD, Compare to cluster

O08354 acetohydroxy-acid synthase small subunit (EC 2.2.1.6) from Methanococcus aeolicus
PFams: ACT, ACT_5, ALS_ss_C
Heteromeric, 169 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 4 82-87 amino acids (not heteromeric)

A0A0H2X4P1 Acetolactate synthase isozyme II small subunit
85 amino acids: PaperBLAST, CDD, Compare to cluster

b3769 acetolactate synthase II subunit IlvM (EC 2.2.1.6) from Escherichia coli K-12 substr. MG1655
P0ADG1 acetolactate synthase (subunit 1/2) (EC 2.2.1.6) from Escherichia coli
PFams: ACT_5
87 amino acids: PaperBLAST, CDD, Compare to cluster

A0A154R0Y7 Acetolactate synthase small subunit {ECO:0000256|RuleBase:RU368092}; Short=AHAS {ECO:0000256|RuleBase:RU368092}; Short=ALS {ECO:0000256|RuleBase:RU368092}; EC=2.2.1.6 {ECO:0000256|RuleBase:RU368092}; Acetohydroxy-acid synthase small subunit {ECO:0000256|RuleBase:RU368092}
82 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/2 heteromeric)

A0A161J739 Acetolactate synthase 3 regulatory subunit domain protein
83 amino acids: PaperBLAST, CDD

A0A7J3AYJ4 Uncharacterized protein
88 amino acids: PaperBLAST, CDD