Clusters of Characterized Proteins

GapMind for catabolism of small carbon sources

Clusters of Characterized Proteins

Clustering the characterized proteins for ech (2-methyl-3-hydroxybutyryl-CoA hydro-lyase) in L-isoleucine catabolism

Or see other characterized proteins similar to ech

Or see all steps for L-isoleucine catabolism

Or cluster curated proteins matching a keyword

Definition of ech

Curated proteins or TIGRFams with EC 4.2.1.17
Curated sequence GFF2389: Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17)
Ignore hits to items matching 4.2.1.150 when looking for 'other' hits
Ignore hits to Q9SE41 when looking for 'other' hits (enoyl-CoA hydratase (EC 4.2.1.17))
Ignore hits to MONOMER-11695 when looking for 'other' hits (enoyl-CoA hydratase subunit (EC 4.2.1.17))
Ignore hits to PP_3358 when looking for 'other' hits (hydroxycinnamoyl-CoA hydratase-lyase (EC 4.2.1.17; EC 4.1.2.-))
Comment: Psest_2437 (GFF2389) is the enoyl-CoA hydrotase for both isoleucine and valine degradation. A paper claiming that Q9SE41 (PMID:23809632) is ech relies on homology modeling, and it could well be a hydrolase instead; it is 69% identical to the hydrolase from Arabidopsis (Q9LKJ1). And MetaCyc linked Q51969 (MONOMER-11695) from P. putida to ech, but the sequence shown in the paper (PMID:12115060, Fig. 3) is different. And PP_3358 (hydroxycinnamoyl-CoA hydratase-lyase) was incorrectly given this EC number.

Fetched 36 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 34 of these sequences

Found 2 clusters of similar sequences. Another 3 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 254-296 amino acids (not heteromeric)

Q9I5I4 enoyl-CoA hydratase (EC 4.2.1.17) from Pseudomonas aeruginosa
PFams: ECH_1, ECH_2
272 amino acids: PaperBLAST, CDD, Compare to cluster

ECHM_RAT / P14604 Enoyl-CoA hydratase, mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17 from Rattus norvegicus (Rat)
P14604 enoyl-CoA hydratase (EC 4.2.1.17); DELTA3,5-DELTA2,4-dienoyl-CoA isomerase (EC 5.3.3.21) from Rattus norvegicus
PFams: ECH_1, ECH_2
290 amino acids: PaperBLAST, CDD, Compare to cluster

P30084 mitochondrial enoyl-CoA hydratase (EC 4.2.1.17) from Homo sapiens
ECHM_HUMAN / P30084 Enoyl-CoA hydratase, mitochondrial; Enoyl-CoA hydratase 1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17 from Homo sapiens (Human)
P30084 enoyl-CoA hydratase (EC 4.2.1.17) from Homo sapiens
PFams: ECH_1, ECH_2
290 amino acids: PaperBLAST, CDD, Compare to cluster

PAAF_ECOLI / P76082 2,3-dehydroadipyl-CoA hydratase; Enoyl-CoA hydratase; EC 4.2.1.17 from Escherichia coli (strain K12)
P76082 enoyl-CoA hydratase (EC 4.2.1.17) from Escherichia coli
PFams: ECH_1, ECH_2
255 amino acids: PaperBLAST, CDD, Compare to cluster

PAAG_ECOLI / P77467 1,2-epoxyphenylacetyl-CoA isomerase; EC 5.3.3.18 (from HMM only) from Escherichia coli (strain K12)
P77467 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) from Escherichia coli
PFams: ECH_1, ECH_2
262 amino acids: PaperBLAST, CDD, Compare to cluster

DPGD_AMYOR / G4V4T7 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis)
PFams: ECH_1, ECH_2
265 amino acids: PaperBLAST, CDD, Compare to cluster

FADB_BACSU / P94549 Probable enoyl-CoA hydratase; EC 4.2.1.17 from Bacillus subtilis (strain 168)
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD, Compare to cluster

FER4_USTMA / Q4PEN0 Enoyl-CoA isomerase/hydratase fer4; Fe-regulated protein 4; Ferrichrome A biosynthesis protein fer4; EC 4.2.1.17 from Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
PFams: ECH_1, ECH_2
274 amino acids: PaperBLAST, CDD, Compare to cluster

ACTT3_ALTAL / Q589W8 Enoyl-CoA hydratase ACTT3; ACT-toxin biosynthesis protein 3; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: ECH_1, ECH_2
296 amino acids: PaperBLAST, CDD, Compare to cluster

ECH17_MYCBO / Q7TXE1 Probable enoyl-CoA hydratase echA17; EC 4.2.1.17 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
PFams: ECH_1, ECH_2
254 amino acids: PaperBLAST, CDD, Compare to cluster

AFT31_ALTAL / Q96VB3 Enoyl-CoA hydratase AFT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: ECH_1, ECH_2
296 amino acids: PaperBLAST, CDD, Compare to cluster

AKT32_ALTAL / Q9P4U7 Enoyl-CoA hydratase AKT3-2; AK-toxin biosynthesis protein 3-2; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: ECH_1, ECH_2
296 amino acids: PaperBLAST, CDD, Compare to cluster

AKT31_ALTAL / Q9P4U9 Enoyl-CoA hydratase AKT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: ECH_1, ECH_2
296 amino acids: PaperBLAST, CDD, Compare to cluster

Q58DM8 short chain enoyl-CoA hydratase subunit (EC 4.2.1.17) from Bos taurus
PFams: ECH_1, ECH_2
290 amino acids: PaperBLAST, CDD, Compare to cluster

Q845K2 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Pseudomonas sp. Y2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD, Compare to cluster

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD, Compare to cluster

Psest_2437 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) from Pseudomonas stutzeri RCH2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 714-763 amino acids (5/16 heteromeric)

ECHP_RAT / P07896 Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Rattus norvegicus (Rat)
P07896 enoyl-CoA hydratase (EC 4.2.1.17) from Rattus norvegicus
RF|NP_598290.1 noyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase/3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
722 amino acids: PaperBLAST, CDD, Compare to cluster

ECHA_HUMAN / P40939 Trifunctional enzyme subunit alpha, mitochondrial; 78 kDa gastrin-binding protein; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Homo sapiens (Human)
P40939 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211) from Homo sapiens
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
Heteromeric, 763 amino acids: PaperBLAST, CDD, Compare to cluster

ECHA_RAT / Q64428 Trifunctional enzyme subunit alpha, mitochondrial; Monolysocardiolipin acyltransferase; TP-alpha; EC 2.3.1.-; EC 4.2.1.17; EC 1.1.1.211 from Rattus norvegicus (Rat)
Q64428 long-chain-3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.211); acetyl-CoA C-acyltransferase (EC 2.3.1.16) from Rattus norvegicus
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
Heteromeric, 763 amino acids: PaperBLAST, CDD, Compare to cluster

MFP2_ARATH / Q9ZPI5 Peroxisomal fatty acid beta-oxidation multifunctional protein MFP2; AtMPF2; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Arabidopsis thaliana (Mouse-ear cress)
Q9ZPI5 enoyl-CoA hydratase (EC 4.2.1.17) from Arabidopsis thaliana
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
725 amino acids: PaperBLAST, CDD, Compare to cluster

b2341 3-hydroxyacyl-CoA dehydrogenase FadJ (EC 1.1.1.35; EC 1.1.1.211; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8) from Escherichia coli K-12 substr. MG1655
P77399 3-hydroxyacyl-CoA dehydrogenase FadJ (EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8) from Escherichia coli (strain K12)
FADJ_ECOLI / P77399 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 1.1.1.35 from Escherichia coli (strain K12)
RF|NP_416843 fatty acid oxidation complex subunit alpha; EC 1.1.1.35; EC 4.2.1.17; EC 5.1.2.3 from Escherichia coli K12
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
Heteromeric, 714 amino acids: PaperBLAST, CDD, Compare to cluster

b3846 dodecenoyl-CoA δ-isomerase, enoyl-CoA hydratase, 3-hydroxybutyryl-CoA epimerase, 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35; EC 1.1.1.211; EC 4.2.1.17; EC 5.3.3.8; EC 5.1.2.3) from Escherichia coli K-12 substr. MG1655
P21177 dodecenoyl-CoA δ-isomerase, enoyl-CoA hydratase, 3-hydroxybutyryl-CoA epimerase, 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35; EC 4.2.1.17; EC 5.3.3.8; EC 5.1.2.3) from Escherichia coli (strain K12)
FADB_ECOLI / P21177 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Escherichia coli (strain K12)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
Heteromeric, 729 amino acids: PaperBLAST, CDD, Compare to cluster

FADB_PSEFR / P28793 Fatty acid oxidation complex subunit alpha; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Pseudomonas fragi
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
Heteromeric, 715 amino acids: PaperBLAST, CDD, Compare to cluster

Q08426 peroxisomal bifunctional enzyme (EC 4.2.1.17; EC 1.1.1.35) from Homo sapiens
ECHP_HUMAN / Q08426 Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 from Homo sapiens (Human)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH, 3HCDH
723 amino acids: PaperBLAST, CDD, Compare to cluster

MFPA_CUCSA / Q39659 Glyoxysomal fatty acid beta-oxidation multifunctional protein MFP-a; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Cucumis sativus (Cucumber)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
725 amino acids: PaperBLAST, CDD, Compare to cluster

MFP_ORYSJ / Q8W1L6 Peroxisomal fatty acid beta-oxidation multifunctional protein; MFP; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Oryza sativa subsp. japonica (Rice)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
726 amino acids: PaperBLAST, CDD, Compare to cluster

Q9ZPI6 peroxisomal fatty acid β-oxidation multifunctional protein AIM1 (EC 4.2.1.17) from Arabidopsis thaliana
AIM1_ARATH / Q9ZPI6 Peroxisomal fatty acid beta-oxidation multifunctional protein AIM1; Protein ABNORMAL INFLORESCENCE MERISTEM 1; AtAIM1; EC 4.2.1.17; EC 5.1.2.3; EC 5.3.3.8; EC 1.1.1.35 from Arabidopsis thaliana (Mouse-ear cress)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
721 amino acids: PaperBLAST, CDD, Compare to cluster

Q88L02 FadB (EC 5.3.3.8; EC 1.1.1.35; EC 5.1.2.3; EC 4.2.1.17; EC 1.1.1.36) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
715 amino acids: PaperBLAST, CDD, Compare to cluster

Q9HZJ2 fatty acid oxidation complex α subunit (EC 1.1.1.35; EC 4.2.1.17) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
715 amino acids: PaperBLAST, CDD, Compare to cluster

Shewana3_0024 Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) from Shewanella sp. ANA-3
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
716 amino acids: PaperBLAST, CDD, Compare to cluster

HP15_1512 Enoyl-CoA hydratase (EC 4.2.1.17) from Marinobacter adhaerens HP15
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
715 amino acids: PaperBLAST, CDD, Compare to cluster

Sama_0032 Enoyl-CoA hydratase (EC 4.2.1.17); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) from Shewanella amazonensis SB2B
PFams: ECH_1, ECH_2, 3HCDH_N, 3HCDH
716 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/3 heteromeric)

Q0KEG0 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); enoyl-CoA hydratase (EC 4.2.1.17) from Cupriavidus necator
PFams: 3HCDH_N, 3HCDH, ECH_1, ECH_2
807 amino acids: PaperBLAST, CDD

DPGB_AMYOR / G4V4T5 Enoyl-CoA-hydratase; EC 4.2.1.17 from Amycolatopsis orientalis (Nocardia orientalis)
217 amino acids: PaperBLAST, CDD

ECH14_MYCBO / P64019 Probable enoyl-CoA hydratase echA14; EC 4.2.1.17 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
PFams: ECH_1, ECH_2
256 amino acids: PaperBLAST, CDD
(similar to BRENDA::P76082 from Cluster 1, but similarity to seed sequence is below thresholds)