Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for leuA (2-isopropylmalate synthase) in L-leucine biosynthesis

Or see other characterized proteins similar to leuA

Or see all steps for L-leucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of leuA

Curated proteins or TIGRFams with EC 2.3.3.13
UniProt sequence L0G5E1_ECHVK: SubName: Full=Isopropylmalate/homocitrate/citramalate synthase {ECO:0000313|EMBL:AGA80045.1};
UniProt sequence A0A1X9Z750_9SPHI: SubName: Full=2-isopropylmalate synthase {ECO:0000313|EMBL:ARS41048.1};
Ignore hits to items matching isopropylmalate synthase when looking for 'other' hits
Ignore hits to P85362 when looking for 'other' hits (2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13)
Ignore hits to S3D9F8 when looking for 'other' hits (Isopropyl malate synthase gloH; L-homotyrosine biosynthesis sub-cluster protein gloH; Pneumocandin biosynthesis cluster protein H; EC 2.3.3.13)
Ignore hits to K0E4E5 when looking for 'other' hits (Isopropyl malate synthase htyA; L-homotyrosine biosynthetic cluster protein A; EC 2.3.3.13. 2-(4-hydroxybenzyl)-malate synthase)
Comment: Ignore CharProtDB entries with no EC number. Add Echvi_3833 (L0G5E1_ECHVK) and CA265_RS15855 (A0A1X9Z750_9SPHI), which are supported by fitness data. P85362 is ignored because it is just 9 amino acids. S3D9F8 and K0E4E5 are involved in secondary metabolite synthesis and it is not clear if they actually have this activity, so they are ignored.

Fetched 31 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 31 of these sequences

Found 3 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 386-635 amino acids (not heteromeric)

LEU11_LEPIN / Q8F445 2-isopropylmalate synthase 1; Alpha-IPM synthase 1; Alpha-isopropylmalate synthase 1; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
PFams: HMGL-like, LeuA_dimer
501 amino acids: PaperBLAST, CDD, Compare to cluster

D0VY45 homocitrate synthase (EC 2.3.3.14) (from HMM only) from Lotus japonicus
PFams: HMGL-like, LeuA_dimer
540 amino acids: PaperBLAST, CDD, Compare to cluster

K4C627 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum
PFams: HMGL-like, LeuA_dimer
612 amino acids: PaperBLAST, CDD, Compare to cluster

K4CJ56 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum lycopersicum
PFams: HMGL-like
445 amino acids: PaperBLAST, CDD, Compare to cluster

Q2QXY9 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group
PFams: HMGL-like, LeuA_dimer
635 amino acids: PaperBLAST, CDD, Compare to cluster

Q2RAP8 2-isopropylmalate synthase (EC 2.3.3.13) from Oryza sativa Japonica Group
PFams: HMGL-like, LeuA_dimer
635 amino acids: PaperBLAST, CDD, Compare to cluster

Q30DX9 2-isopropylmalate synthase (EC 2.3.3.13) from Brassica insularis
PFams: HMGL-like, LeuA_dimer
623 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_METJA / Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Q58595 2-isopropylmalate synthase (EC 2.3.3.13) from Methanocaldococcus jannaschii
PFams: HMGL-like, LeuA_dimer
518 amino acids: PaperBLAST, CDD, Compare to cluster

Q72JC9 2-isopropylmalate synthase (EC 2.3.3.13) from Thermus thermophilus
PFams: HMGL-like, LeuA_dimer
520 amino acids: PaperBLAST, CDD, Compare to cluster

LEU12_ARATH / Q9C550 2-isopropylmalate synthase 2, chloroplastic; 2-isopropylmalate synthase 1; Methylthioalkylmalate synthase-like 3; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress)
Q9C550 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana
PFams: HMGL-like, LeuA_dimer
631 amino acids: PaperBLAST, CDD, Compare to cluster

Q9FG67 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
MAM1_ARATH / Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress)
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD, Compare to cluster

Q9FN52 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
MAM3_ARATH / Q9FN52 Methylthioalkylmalate synthase 3, chloroplastic; 2-isopropylmalate synthase 2; Methylthioalkylmalate synthase-like; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress)
Q9FN52 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
PFams: HMGL-like
503 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_NEIMB / Q9JZG1 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Neisseria meningitidis serogroup B (strain MC58)
Q9JZG1 2-isopropylmalate synthase (EC 2.3.3.13) from Neisseria meningitidis
PFams: HMGL-like, LeuA_dimer
517 amino acids: PaperBLAST, CDD, Compare to cluster

LEU11_ARATH / Q9LPR4 2-isopropylmalate synthase 1, chloroplastic; Methylthioalkylmalate synthase-like 4; EC 2.3.3.13 from Arabidopsis thaliana (Mouse-ear cress)
Q9LPR4 2-isopropylmalate synthase (EC 2.3.3.13) from Arabidopsis thaliana
PFams: HMGL-like, LeuA_dimer
631 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_SALTY / P15875 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
PFams: HMGL-like, LeuA_dimer
523 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_SOYBN / Q39891 Probable 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; Late nodulin-56; N-56; EC 2.3.3.13 from Glycine max (Soybean) (Glycine hispida)
PFams: HMGL-like, LeuA_dimer
565 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
PFams: HMGL-like
386 amino acids: PaperBLAST, CDD, Compare to cluster

A0A1X9Z750_9SPHI 2-isopropylmalate synthase
386 amino acids: PaperBLAST, CDD, Compare to cluster

L0G5E1_ECHVK Isopropylmalate/homocitrate/citramalate synthase
390 amino acids: PaperBLAST, CDD, Compare to cluster

b0074 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli K-12 substr. MG1655
P09151 2-isopropylmalate synthase (EC 2.3.3.13) from Escherichia coli (strain K12)
PFams: HMGL-like, LeuA_dimer
523 amino acids: PaperBLAST, CDD, Compare to cluster

BT1861 2-isopropylmalate synthase (EC 2.3.3.13) from Bacteroides thetaiotaomicron VPI-5482
PFams: HMGL-like, LeuA_dimer
498 amino acids: PaperBLAST, CDD, Compare to cluster

Echvi_3833 2-isopropylmalate synthase (EC 2.3.3.13) from Echinicola vietnamensis KMM 6221, DSM 17526
PFams: HMGL-like
390 amino acids: PaperBLAST, CDD, Compare to cluster

CA265_RS15855 2-isopropylmalate synthase (EC 2.3.3.13) from Pedobacter sp. GW460-11-11-14-LB5
PFams: HMGL-like
386 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 582-644 amino acids (not heteromeric)

LEU1_MYCTO / P9WQB2 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
LEU1_MYCTU / P9WQB3 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WQB3 2-isopropylmalate synthase (EC 2.3.3.13) from Mycobacterium tuberculosis
PFams: HMGL-like, LeuA_dimer
644 amino acids: PaperBLAST, CDD, Compare to cluster

AMT7_ALTAL / C9K7B8 Isopropyl malate synthase AMT7; AM-toxin biosynthesis protein 7; EC 2.3.3.13 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: HMGL-like
582 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_YEAST / P06208 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: HMGL-like, LeuA_dimer
619 amino acids: PaperBLAST, CDD, Compare to cluster

LEU1_CORGL / P42455 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
PFams: HMGL-like, LeuA_dimer
616 amino acids: PaperBLAST, CDD, Compare to cluster

LEU9_YEAST / Q12166 2-isopropylmalate synthase 2, mitochondrial; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; Alpha-isopropylmalate synthase II; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: HMGL-like, LeuA_dimer
604 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 394-428 amino acids (not heteromeric)

B0SN40 2-isopropylmalate synthase (EC 2.3.3.13) from Leptospira biflexa
PFams: HMGL-like
394 amino acids: PaperBLAST, CDD, Compare to cluster

A0A0G2T6D7 2-isopropylmalate synthase (EC 2.3.3.13) from Solanum pennellii
PFams: HMGL-like
394 amino acids: PaperBLAST, CDD, Compare to cluster

LEU12_LEPIN / Q8F8T4 2-isopropylmalate synthase 2; Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2; EC 2.3.3.13 from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
PFams: HMGL-like
428 amino acids: PaperBLAST, CDD, Compare to cluster