Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for leuC (3-isopropylmalate dehydratase / citramalate isomerase, large subunit) in L-leucine biosynthesis

Or see other characterized proteins similar to leuC

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Or cluster curated proteins matching a keyword

Definition of leuC

Curated proteins matching citramalate isomerase large subunit
Curated proteins matching 3-isopropylmalate dehydratase large subunit
Curated proteins matching 3-isopropylmalate dehydratase%LeuC
HMM TIGR00170
HMM TIGR02083
HMM TIGR02086
Ignore hits to Q0QLE2 when looking for 'other' hits (2,3-dimethylmalate dehydratase large subunit; EC 4.2.1.85. dimethylmaleate hydratase subunit A (EC 4.2.1.85))
Ignore hits to items matching EC 4.2.1.33 when looking for 'other' hits
Ignore hits to items matching EC 4.2.1.35 when looking for 'other' hits
UniProt sequence LEUC_DESVH: RecName: Full=3-isopropylmalate dehydratase large subunit {ECO:0000255|HAMAP-Rule:MF_01027}; EC=4.2.1.33 {ECO:0000255|HAMAP-Rule:MF_01027}; AltName: Full=Alpha-IPM isomerase {ECO:0000255|HAMAP-Rule:MF_01027}; Short=IPMI {ECO:0000255|HAMAP-Rule:MF_01027}; AltName: Full=Isopropylmalate isomerase {ECO:0000255|HAMAP-Rule:MF_01027};
Ignore hits to CH_122621 when looking for 'other' hits (alpha isopropylmalate isomerase (Eurofung))
Ignore hits to S3E7P8 when looking for 'other' hits (3-isopropylmalate dehydratase large subunit gloJ; L-homotyrosine biosynthesis sub-cluster protein gloJ; Pneumocandin biosynthesis cluster protein J; EC 4.2.1.33)
UniProt sequence A0A1X9Z7T5: RecName: Full=3-isopropylmalate dehydratase large subunit {ECO:0000256|HAMAP-Rule:MF_01026}; EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01026}; AltName: Full=Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01026}; Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01026}; AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01026};
Comment: In leucine synthesis, LeuCD allows the dehydration of 2-isopropylmalate and hydration to 3-isopropylmalate. Similarly, many of these enzymes allow the isomerization of citramalate to 3-methylmalate via citraconate. Citramalate isomerase is usually given as EC 4.2.1.35, as opposed to 4.2.1.33 for traditional leuCD. However, many the bacteria with the citramalate pathway appeared to have "typical" leuBCD (i.e., Desulfovibrio vulgaris Hildenborough, Desulfovibrio vulgaris Miyazaki F, Bacteroides thetaiotaomicron, Magnetospirillum magneticum AMB-1, and Synechococcus elongatus PCC 7942). So we do not try to distinguish between 3-isoprpylmalate dehydratase and citramalate isomerase. Ignore a 2,3-methylmalate dehydratase (Q0QLE2,Q0QLE1) which is >50% identical to leuCD from DvH (DVU2982,DVU2983). Ignore some BRENDA annotations without subunit information, and ignore CharProtDB::CH_122621 (leuCD fusion) which is not actually characterized. DvH leuC (DVU2982) has similarity to both LeuC and to homoaconitase, and fitness data confirms its role in amino acid biosynthesis, so explicitly include it. CA265_RS15830 (A0A1X9Z7T5) from Pedobacter sp. GW460-11-11-14-LB5 is important for fitness unless amino acids are added. S3E7P8 is annotated as this in SwissProt but we did not find experimental evidence, so it is ignored.

Fetched 12 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 12 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 418-569 amino acids (9/12 heteromeric)

The first sequence in each cluster is the seed.

P81291 isopropylmalate isomerase large subunit (EC 4.2.1.35) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
LEUC_METJA / P81291 Isopropylmalate/citramalate isomerase large subunit; (R)-2-methylmalate dehydratase; (R)-citramalate dehydratase; 3-isopropylmalate dehydratase; Alpha-isopropylmalate dehydratase; Citraconate hydratase; Isopropylmalate isomerase; IPMI; Maleate hydratase; Malease; EC 4.2.1.33; EC 4.2.1.35; EC 4.2.1.31 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
P81291 3-isopropylmalate dehydratase (EC 4.2.1.33); homoaconitate hydratase (EC 4.2.1.36) from Methanocaldococcus jannaschii
PFams: Aconitase, Aconitase
Heteromeric, 424 amino acids: PaperBLAST, CDD, Compare to cluster

Q58409 homoaconitase α subunit (EC 4.2.1.36) (from HMM only) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
HACA_METJA / Q58409 Methanogen homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.114 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Q58409 methanogen homoaconitase (EC 4.2.1.114) from Methanocaldococcus jannaschii
CH_091727 homoaconitase; EC 4.2.1.-; EC 4.2.1.36 from Methanocaldococcus jannaschii
PFams: Aconitase, Aconitase
Heteromeric, 420 amino acids: PaperBLAST, CDD, Compare to cluster

Q94AR8 isopropylmalate isomerase large subunit (EC 4.2.1.33) from Arabidopsis thaliana
LEUC_ARATH / Q94AR8 3-isopropylmalate dehydratase large subunit, chloroplastic; 2-(omega-methylthio)alkylmalate dehydratase large subunit; AtLEUC; Isopropylmalate isomerase large subunit 1; AtIIL1; IPMI LSU1; Methylthioalkylmalate isomerase large subunit; MAM-IL; EC 4.2.1.33; EC 4.2.1.170 from Arabidopsis thaliana (Mouse-ear cress)
Q94AR8 2-(omega-methylthio)alkylmalate dehydratase (subunit 2/2) (EC 4.2.1.170); 3-isopropylmalate dehydratase (EC 4.2.1.33) from Arabidopsis thaliana
PFams: Aconitase, Aconitase
Heteromeric, 509 amino acids: PaperBLAST, CDD, Compare to cluster

b0072 3-isopropylmalate dehydratase subunit LeuC (EC 4.2.1.33) from Escherichia coli K-12 substr. MG1655
P0A6A6 3-isopropylmalate dehydratase subunit LeuC (EC 4.2.1.33) from Escherichia coli (strain K12)
ECOCYC|LEUC-MONOMER 3-isopropylmalate dehydratase large subunit; EC 4.2.1.33 from Escherichia coli K12
PFams: Aconitase
Heteromeric, 466 amino acids: PaperBLAST, CDD, Compare to cluster

LEUC1_SALTY / P15717 3-isopropylmalate dehydratase large subunit 1; Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1; EC 4.2.1.33 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
PFams: Aconitase
Heteromeric, 466 amino acids: PaperBLAST, CDD, Compare to cluster

LEUC_CORGL / P58946 3-isopropylmalate dehydratase large subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
PFams: Aconitase
Heteromeric, 481 amino acids: PaperBLAST, CDD, Compare to cluster

LEUC_MYCBO / Q7TXH6 3-isopropylmalate dehydratase large subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
PFams: Aconitase
Heteromeric, 473 amino acids: PaperBLAST, CDD, Compare to cluster

Q9ZNE0 homoaconitase large subunit (EC 4.2.1.36) (from HMM only) from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
HACA_THET2 / Q9ZNE0 Homoaconitase large subunit; HACN; Homoaconitate hydratase; EC 4.2.1.36 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
PFams: Aconitase
Heteromeric, 418 amino acids: PaperBLAST, CDD, Compare to cluster

A0A1X9Z7T5 3-isopropylmalate dehydratase large subunit {ECO:0000256|HAMAP-Rule:MF_01026}; EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01026}; Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01026}; Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01026}; AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01026}
470 amino acids: PaperBLAST, CDD, Compare to cluster

LEUC_DESVH 3-isopropylmalate dehydratase large subunit {ECO:0000255|HAMAP-Rule:MF_01027}; EC=4.2.1.33 {ECO:0000255|HAMAP-Rule:MF_01027}; Alpha-IPM isomerase {ECO:0000255|HAMAP-Rule:MF_01027}; Short=IPMI {ECO:0000255|HAMAP-Rule:MF_01027}; AltName: Full=Isopropylmalate isomerase {ECO:0000255|HAMAP-Rule:MF_01027}
419 amino acids: PaperBLAST, CDD, Compare to cluster

B2J8P2 benzylmalate isomerase (from HMM only) from Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
PFams: Aconitase_C, Aconitase, Aconitase
569 amino acids: PaperBLAST, CDD, Compare to cluster

DVU2982 isopropylmalate/citramalate isomerase large subunit LeuC (EC 4.2.1.33; EC 4.2.1.35) from Desulfovibrio vulgaris Hildenborough JW710
PFams: Aconitase, Aconitase
Heteromeric, 419 amino acids: PaperBLAST, CDD, Compare to cluster