Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for asp-kinase (aspartate kinase) in L-lysine biosynthesis

Or see other characterized proteins similar to asp-kinase

Or see all steps for L-lysine biosynthesis

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Definition of asp-kinase

Curated proteins or TIGRFams with EC 2.7.2.4
Ignore hits to O63067 when looking for 'other' hits (homoserine dehydrogenase (EC 1.1.1.3))
Ignore hits to Q46133 when looking for 'other' hits (aspartate kinase (EC 2.7.2.4))
Comment: For BRENDA::O63067 -- the paper describes a monofunctional hom but the sequence of O63067 is much longer and has a close homolog of functional aspartate kinase (due to alternative splicing?). In Corynebacterium, aspartate kinase has two subunits, both apparently encoded by the same gene by using start codons (PMID:1956296); Q46133 is the shorter regulatory subunit and lacks the catalytic domain, so it does not suffice for activity and is ignored.

Fetched 36 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 33 of these sequences

Found 3 clusters of similar sequences. Another 4 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 404-600 amino acids (2/22 heteromeric)

Q57991 aspartate kinase (EC 2.7.2.4) from Methanocaldococcus jannaschii
PFams: AA_kinase, ACT_7, ACT, ACT_7, ACT
473 amino acids: PaperBLAST, CDD, Compare to cluster

AK2_ARATH / O23653 Aspartokinase 2, chloroplastic; Aspartate kinase 2; EC 2.7.2.4 from Arabidopsis thaliana (Mouse-ear cress)
O23653 aspartate kinase (EC 2.7.2.4) from Arabidopsis thaliana
PFams: AA_kinase
544 amino acids: PaperBLAST, CDD, Compare to cluster

O69077 aspartate kinase (EC 2.7.2.4) from Pseudomonas aeruginosa
PFams: AA_kinase, ACT, ACT_7
412 amino acids: PaperBLAST, CDD, Compare to cluster

P08495 aspartate kinase II α subunit (EC 2.7.2.4) from Bacillus subtilis (strain 168)
P08495 aspartate kinase II β subunit (EC 2.7.2.4) from Bacillus subtilis (strain 168)
AK2_BACSU / P08495 Aspartokinase 2; Aspartate kinase 2; Aspartokinase II; EC 2.7.2.4 from Bacillus subtilis (strain 168)
P08495 aspartate kinase (EC 2.7.2.4) from Bacillus subtilis
GB|CAA99580.1 aspartate kinase 2; EC 2.7.2.4 from Bacillus subtilis
PFams: AA_kinase, ACT, ACT_7
Heteromeric, 408 amino acids: PaperBLAST, CDD, Compare to cluster

b4024 aspartate kinase III (EC 2.7.2.4) from Escherichia coli K-12 substr. MG1655
P08660 aspartate kinase III (EC 2.7.2.4) from Escherichia coli (strain K12)
AK3_ECOLI / P08660 Lysine-sensitive aspartokinase 3; Aspartate kinase III; AKIII; Lysine-sensitive aspartokinase III; EC 2.7.2.4 from Escherichia coli (strain K12)
P08660 aspartate kinase (EC 2.7.2.4) from Escherichia coli
PFams: AA_kinase
449 amino acids: PaperBLAST, CDD, Compare to cluster

AK_YEAST / P10869 Aspartokinase; Aspartate kinase; EC 2.7.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
P10869 aspartate kinase (EC 2.7.2.4) from Saccharomyces cerevisiae
PFams: AA_kinase, ACT_7
527 amino acids: PaperBLAST, CDD, Compare to cluster

P26512 aspartokinase α subunit (EC 2.7.2.4) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
P26512 aspartokinase β subunit (EC 2.7.2.4) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
AK_CORGL / P26512 Aspartokinase; Aspartate kinase; EC 2.7.2.4 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
P26512 aspartate kinase (EC 2.7.2.4) from Corynebacterium glutamicum
CH_091815 aspartate kinase 2; EC 2.7.2.4 from Corynebacterium glutamicum
PFams: AA_kinase, ACT, ACT_7
Heteromeric, 421 amino acids: PaperBLAST, CDD, Compare to cluster

P74569 aspartate kinase (EC 2.7.2.4) from Synechocystis sp.
PFams: AA_kinase, ACT, ACT_7, ACT, ACT_7
600 amino acids: PaperBLAST, CDD, Compare to cluster

AK_MYCTU / P9WPX3 Aspartokinase; Aspartate kinase; ASK; EC 2.7.2.4 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WPX3 aspartate kinase (EC 2.7.2.4) from Mycobacterium tuberculosis
PFams: AA_kinase, ACT, ACT_7
421 amino acids: PaperBLAST, CDD, Compare to cluster

Q5JK18 aspartate kinase (EC 2.7.2.4) from Oryza sativa Japonica Group
PFams: AA_kinase
570 amino acids: PaperBLAST, CDD, Compare to cluster

Q97MC0 aspartate kinase (EC 2.7.2.4) from Clostridium acetobutylicum
PFams: AA_kinase, ACT
437 amino acids: PaperBLAST, CDD, Compare to cluster

AK1_ARATH / Q9LYU8 Aspartokinase 1, chloroplastic; Aspartate kinase 1; EC 2.7.2.4 from Arabidopsis thaliana (Mouse-ear cress)
Q9LYU8 aspartate kinase (EC 2.7.2.4) from Arabidopsis thaliana
PFams: AA_kinase
569 amino acids: PaperBLAST, CDD, Compare to cluster

Q9RUL9 aspartate kinase (EC 2.7.2.4) from Deinococcus radiodurans
PFams: AA_kinase, ACT_7, ACT_7
473 amino acids: PaperBLAST, CDD, Compare to cluster

Q9S702 aspartate kinase (EC 2.7.2.4) from Arabidopsis thaliana
AK3_ARATH / Q9S702 Aspartokinase 3, chloroplastic; Aspartate kinase 3; EC 2.7.2.4 from Arabidopsis thaliana (Mouse-ear cress)
Q9S702 aspartate kinase (EC 2.7.2.4) from Arabidopsis thaliana
PFams: AA_kinase
559 amino acids: PaperBLAST, CDD, Compare to cluster

CH_090816 aspartate kinase 1; EC 2.7.2.4 from Bacillus subtilis
PFams: AA_kinase, ACT_7
404 amino acids: PaperBLAST, CDD, Compare to cluster

AKLYS_STUS1 / A4VJB4 Aspartate kinase Ask_LysC; Aspartokinase; EC 2.7.2.4 from Stutzerimonas stutzeri (strain A1501) (Pseudomonas stutzeri)
PFams: AA_kinase, ACT, ACT_7
412 amino acids: PaperBLAST, CDD, Compare to cluster

AK_PSEFS / C3JXY0 Aspartate kinase; Aspartokinase; EC 2.7.2.4 from Pseudomonas fluorescens (strain SBW25)
PFams: AA_kinase, ACT, ACT_7
413 amino acids: PaperBLAST, CDD, Compare to cluster

AK_CORFL / P41398 Aspartokinase; Aspartate kinase; EC 2.7.2.4 from Corynebacterium flavescens
GB|AAA23293.1 aspartate kinase; EC 2.7.2.4 from Corynebacterium flavescens
PFams: AA_kinase, ACT, ACT_7
421 amino acids: PaperBLAST, CDD, Compare to cluster

AK_THET2 / P61488 Aspartokinase; Aspartate kinase; ASK; EC 2.7.2.4 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
PFams: AA_kinase, ACT, ACT_7
405 amino acids: PaperBLAST, CDD, Compare to cluster

AK_THETH / P61489 Aspartokinase; Aspartate kinase; AK; ASK; Threonine-sensitive AK; ThrA; EC 2.7.2.4 from Thermus thermophilus
P61489 aspartate kinase (EC 2.7.2.4) from Thermus thermophilus
PFams: AA_kinase, ACT, ACT_7
405 amino acids: PaperBLAST, CDD, Compare to cluster

AK_PSEPK / Q88EI9 Aspartate kinase; Aspartokinase; EC 2.7.2.4 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
PFams: AA_kinase, ACT, ACT_7
411 amino acids: PaperBLAST, CDD, Compare to cluster

Q04795 aspartokinase I (alpha and beta subunits) (EC 2.7.2.4) from Bacillus subtilis (strain 168)
PFams: AA_kinase, ACT_7
404 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 810-916 amino acids (not heteromeric)

b0002 fused aspartate kinase/homoserine dehydrogenase 1 (EC 2.7.2.4; EC 1.1.1.3) from Escherichia coli K-12 substr. MG1655
P00561 fused aspartate kinase/homoserine dehydrogenase 1 (EC 2.7.2.4; EC 1.1.1.3) from Escherichia coli (strain K12)
P00561 homoserine dehydrogenase (EC 1.1.1.3); aspartate kinase (EC 2.7.2.4) from Escherichia coli
PFams: AA_kinase, ACT_7, ACT_7, NAD_binding_3, Homoserine_dh
820 amino acids: PaperBLAST, CDD, Compare to cluster

AKH2_ARATH / O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress)
PFams: AA_kinase, ACT_7, ACT, NAD_binding_3, Homoserine_dh
916 amino acids: PaperBLAST, CDD, Compare to cluster

AKH1_ARATH / Q9SA18 Bifunctional aspartokinase/homoserine dehydrogenase 1, chloroplastic; AK-HD 1; AK-HSDH 1; Beta-aspartyl phosphate homoserine 1; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress)
PFams: AA_kinase, ACT_7, ACT, NAD_binding_3, Homoserine_dh
911 amino acids: PaperBLAST, CDD, Compare to cluster

b3940 fused aspartate kinase/homoserine dehydrogenase 2 (EC 2.7.2.4; EC 1.1.1.3) from Escherichia coli K-12 substr. MG1655
P00562 fused aspartate kinase/homoserine dehydrogenase 2 (EC 2.7.2.4; EC 1.1.1.3) from Escherichia coli (strain K12)
PFams: AA_kinase, NAD_binding_3, Homoserine_dh
810 amino acids: PaperBLAST, CDD, Compare to cluster

BT2403 aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) from Bacteroides thetaiotaomicron VPI-5482
PFams: AA_kinase, ACT_7, ACT_7, NAD_binding_3, Homoserine_dh
811 amino acids: PaperBLAST, CDD, Compare to cluster

Echvi_2000 aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) from Echinicola vietnamensis KMM 6221, DSM 17526
PFams: AA_kinase, ACT_7, NAD_binding_3, Homoserine_dh
815 amino acids: PaperBLAST, CDD, Compare to cluster

CA265_RS23475 aspartate kinase; homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) from Pedobacter sp. GW460-11-11-14-LB5
PFams: AA_kinase, ACT_7, ACT, NAD_binding_3, Homoserine_dh
817 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 510-542 amino acids (not heteromeric)

FUB3_FUSO4 / A0A0D2YG09 Aspartate kinase FUB3; Fusaric acid biosynthesis protein 3; EC 2.7.2.4 from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936) (Fusarium vascular wilt of tomato)
PFams: YCII, AA_kinase, ACT_7
542 amino acids: PaperBLAST, CDD, Compare to cluster

FUB3_GIBF5 / S0DVT6 Aspartate kinase FUB3; Fusaric acid biosynthesis protein 3; EC 2.7.2.4 from Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
PFams: AA_kinase, ACT_7
510 amino acids: PaperBLAST, CDD, Compare to cluster

FUB3_GIBM7 / W7MS01 Aspartate kinase FUB3; Fusaric acid biosynthesis protein 3; EC 2.7.2.4 from Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot fungus) (Fusarium verticillioides)
PFams: AA_kinase, ACT_7
510 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/4 heteromeric)

Q9WZ17 homoserine dehydrogenase (EC 1.1.1.3); aspartate kinase (EC 2.7.2.4) from Thermotoga maritima
PFams: NAD_binding_3, Homoserine_dh, AA_kinase, ACT, ACT_7
739 amino acids: PaperBLAST, CDD

AKECT_STUS1 / A4VFY3 Aspartate kinase Ask_Ect; Aspartokinase; EC 2.7.2.4 from Stutzerimonas stutzeri (strain A1501) (Pseudomonas stutzeri)
PFams: AA_kinase, ACT_7, ACT
476 amino acids: PaperBLAST, CDD

P94417 aspartate kinase III (EC 2.7.2.4) from Bacillus subtilis (strain 168)
AK3_BACSU / P94417 Aspartokinase 3; Aspartate kinase 3; Aspartokinase III; EC 2.7.2.4 from Bacillus subtilis (strain 168)
PFams: AA_kinase, ACT_7
454 amino acids: PaperBLAST, CDD
(similar to BRENDA::Q5JK18 from Cluster 1, but similarity to seed sequence is below thresholds)

N515DRAFT_4002 aspartate kinase / diaminopimelate decarboxylase (EC 4.1.1.20; EC 2.7.2.4) from Dyella japonica UNC79MFTsu3.2
PFams: AA_kinase, Orn_Arg_deC_N, Orn_DAP_Arg_deC
853 amino acids: PaperBLAST, CDD