Clusters of Characterized Proteins

Clustering the characterized proteins for metB (cystathionine gamma-synthase) in L-methionine biosynthesis

Or see other characterized proteins similar to metB

Or see all steps for L-methionine biosynthesis

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Definition of metB

• Curated proteins or TIGRFams with EC 2.5.1.48
• Curated sequence O31631: Cystathionine gamma-synthase/O-acetylhomoserine (thiol)-lyase; CGS/OAH thiolyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase; EC 2.5.1.-
• Ignore hits to Q1M0P5 when looking for 'other' hits (Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48. cystathionine gamma-synthase (EC 2.5.1.48))
• Comment: Many metB proteins have some activity as O-succinylhomoserine sulfhydrylase (metZ) as well, and many metZ proteins are annotated with this EC number. So this step may include both enzymes. METI_BACSU (O31631) has activity as CGS but is given a more vague EC number. Q1M0P5 is now thought to be a gamma-lyase instead (see link_RS00540-MONOMER), so it is ignored.

Fetched 14 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 14 of these sequences

Found 3 clusters of similar sequences. Another 1 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 373-415 amino acids (not heteromeric)

A0PKT3 cystathionine gamma-synthase (EC 2.5.1.48) from Mycobacterium ulcerans
PFams: Cys_Met_Meta_PP, Met_gamma_lyase, DegT_DnrJ_EryC1, Aminotran_5
388 amino acids: PaperBLAST, CDD, Compare to cluster

H7C6A2 cystathionine gamma-synthase (EC 2.5.1.48) from Corynebacterium glutamicum
PFams: Cys_Met_Meta_PP, Aminotran_1_2, DegT_DnrJ_EryC1
386 amino acids: PaperBLAST, CDD, Compare to cluster

METI_BACSU / O31631 Cystathionine gamma-synthase/O-acetylhomoserine (thiol)-lyase; CGS/OAH thiolyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase; EC 2.5.1.- from Bacillus subtilis (strain 168)
PFams: Cys_Met_Meta_PP, DegT_DnrJ_EryC1, Aminotran_1_2
373 amino acids: PaperBLAST, CDD, Compare to cluster

b3939 O-succinylhomoserine(thiol)-lyase / O-succinylhomoserine lyase (EC 2.5.1.48) from Escherichia coli K-12 substr. MG1655
P00935 O-succinylhomoserine(thiol)-lyase / O-succinylhomoserine lyase (EC 2.5.1.48) from Escherichia coli (strain K12)
METB_ECOLI / P00935 Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Escherichia coli (strain K12)
PFams: Cys_Met_Meta_PP, Aminotran_1_2
386 amino acids: PaperBLAST, CDD, Compare to cluster

P55218 O-succinyl-L-homoserine sulfhydrylase (EC 2.5.1.48) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
METZ_PSEAE / P55218 O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PFams: Cys_Met_Meta_PP, Aminotran_1_2, Aminotran_5, DegT_DnrJ_EryC1
403 amino acids: PaperBLAST, CDD, Compare to cluster

METB_MYCTU / P9WGB7 Cystathionine gamma-synthase; CGS; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
PFams: Cys_Met_Meta_PP, Met_gamma_lyase, DegT_DnrJ_EryC1, Aminotran_1_2
388 amino acids: PaperBLAST, CDD, Compare to cluster

HSERO_RS16440 O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) from Herbaspirillum seropedicae SmR1
PFams: Cys_Met_Meta_PP, DegT_DnrJ_EryC1, Aminotran_1_2, Aminotran_5
413 amino acids: PaperBLAST, CDD, Compare to cluster

Ac3H11_2452 O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) from Acidovorax sp. GW101-3H11
PFams: Cys_Met_Meta_PP, Aminotran_1_2, Aminotran_5
415 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 610-649 amino acids (not heteromeric)

MET7_SCHPO / O74314 Probable cystathionine gamma-synthase; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: Cys_Met_Meta_PP
610 amino acids: PaperBLAST, CDD, Compare to cluster

P47164 cystathionine γ-synthase (EC 2.5.1.48) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MET7_YEAST / P47164 Cystathionine gamma-synthase; O-succinylhomoserine (thiol)-lyase; Sulfur transfer protein 2; EC 2.5.1.48 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: Cys_Met_Meta_PP
639 amino acids: PaperBLAST, CDD, Compare to cluster

METX_YEAST / Q04533 Putative cystathionine gamma-synthase YML082W; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: Cys_Met_Meta_PP
649 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 534-535 amino acids (not heteromeric)

L0EK12 cystathionine gamma-synthase (EC 2.5.1.48) from Cicer arietinum
PFams: Cys_Met_Meta_PP, Aminotran_1_2, Beta_elim_lyase
534 amino acids: PaperBLAST, CDD, Compare to cluster

L0EKR2 cystathionine gamma-synthase (EC 2.5.1.48) from Lens culinaris
PFams: Cys_Met_Meta_PP, Aminotran_1_2
535 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/1 heteromeric)

P55217 cystathionine γ-synthase (EC 2.5.1.48) from Arabidopsis thaliana
CGS1_ARATH / P55217 Cystathionine gamma-synthase 1, chloroplastic; AtCGS1; METHIONINE OVERACCUMULATION 1; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Arabidopsis thaliana (Mouse-ear cress)
PFams: Cys_Met_Meta_PP
563 amino acids: PaperBLAST, CDD
(similar to BRENDA::L0EK12 from Cluster 3, but similarity to seed sequence is below thresholds)