Clusters of Characterized Proteins

Clustering the characterized proteins for metH (vitamin B12-dependent methionine synthase) in L-methionine biosynthesis

Or see other characterized proteins similar to metH

Or see all steps for L-methionine biosynthesis

Or cluster curated proteins matching a keyword

Definition of metH

• Curated proteins or TIGRFams with EC 2.1.1.13
• Ignore hits to GFF1501 when looking for 'other' hits (ATP-dependent reduction of co(II)balamin (RamA-like) (EC:2.1.1.13))
• Ignore hits to GFF1318 when looking for 'other' hits (Accessory protein for co(II)balamin reduction (DUF1638) (EC:2.1.1.13))
• Ignore hits to GFF1321 when looking for 'other' hits (Methionine synthase component, methyltransferase domain (EC:2.1.1.13))
• Ignore hits to GFF1319 when looking for 'other' hits (Methionine synthase component, B12 binding and B12-binding cap domains (EC:2.1.1.13))
• Ignore hits to GFF1582 when looking for 'other' hits (Methionine synthase component, pterin-binding domain (EC:2.1.1.13))
• UniProt sequence Q72BP9_DESVH: RecName: Full=Methionine synthase {ECO:0000256|ARBA:ARBA00013998}; EC=2.1.1.13 {ECO:0000256|ARBA:ARBA00012032}; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase {ECO:0000256|ARBA:ARBA00031040};
• UniProt sequence B8DKK4_DESVM: RecName: Full=Methionine synthase {ECO:0000256|ARBA:ARBA00013998}; EC=2.1.1.13 {ECO:0000256|ARBA:ARBA00012032}; AltName: Full=5-methyltetrahydrofolate--homocysteine methyltransferase {ECO:0000256|ARBA:ARBA00031040};
• Comment: Desulfovibrio have a somewhat diverged MetH, without the activation domain, but confirmed by cofitness (DVU1585 = Q72BP9_DESVH is cofit with MetF; DvMF_0476 = B8DKK4_DESVM is cofit with a RamA- like activation protein). 3-part split MetH proteins from Phaeobacter are ignored.

Fetched 9 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 9 of these sequences

Found 2 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 768-818 amino acids (not heteromeric)

Q9WYA5 methionine synthase (EC 2.1.1.13) from Thermotoga maritima
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding
768 amino acids: PaperBLAST, CDD, Compare to cluster

B8DKK4_DESVM Methionine synthase; EC=2.1.1.13; 5-methyltetrahydrofolate--homocysteine methyltransferase
818 amino acids: PaperBLAST, CDD, Compare to cluster

Q72BP9_DESVH Methionine synthase; EC=2.1.1.13; 5-methyltetrahydrofolate--homocysteine methyltransferase
804 amino acids: PaperBLAST, CDD, Compare to cluster

DVU1585 Methionine synthase (cobalamin-dependent) (EC 2.1.1.13) from Desulfovibrio vulgaris Hildenborough JW710
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding
804 amino acids: PaperBLAST, CDD, Compare to cluster

DvMF_0476 vitamin B12-dependent methionine synthase without a reactivation domain (EC 2.1.1.13) from Desulfovibrio vulgaris Miyazaki F
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding
818 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 1221-1265 amino acids (not heteromeric)

Q99707 cobalamin-dependent methionine synthase (EC 2.1.1.13) from Homo sapiens
METH_HUMAN / Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human)
Q99707 methionine synthase (EC 2.1.1.13) from Homo sapiens
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding, Met_synt_B12
1265 amino acids: PaperBLAST, CDD, Compare to cluster

METH_RAT / Q9Z2Q4 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Rattus norvegicus (Rat)
Q9Z2Q4 methionine synthase (EC 2.1.1.13) from Rattus norvegicus
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding, Met_synt_B12
1253 amino acids: PaperBLAST, CDD, Compare to cluster

b4019 cobalamin-dependent methionine synthase (EC 2.1.1.13) from Escherichia coli K-12 substr. MG1655
P13009 cobalamin-dependent methionine synthase (EC 2.1.1.13) from Escherichia coli (strain K12)
METH_ECOLI / P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12)
CH_090726 methionine synthase; EC 2.1.1.13 from Escherichia coli K12
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding, Met_synt_B12
1227 amino acids: PaperBLAST, CDD, Compare to cluster

Q8NQD1 cobalamin-dependent methionine synthase (EC 2.1.1.13) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
PFams: S-methyl_trans, Pterin_bind, B12-binding_2, B12-binding, Met_synt_B12
1221 amino acids: PaperBLAST, CDD, Compare to cluster