Clusters of Characterized Proteins
Clustering the characterized proteins for paaZ1 (oxepin-CoA hydrolase) in phenylacetate catabolism
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Definition of paaZ1
- Curated proteins or TIGRFams with EC 3.3.2.12
- UniProt sequence A0A2Z5MCI7: SubName: Full=Enoyl-CoA hydratase {ECO:0000313|EMBL:AXF14912.1}; EC=4.2.1.17 {ECO:0000313|EMBL:AXF14912.1};
- Comment: PaaZ is a fusion protein of hydrolase and aldehyde dehydrogenase domains. However, in many bacteria that use this pathway, the 3-oxo-5,6-didehydrosuberyl-CoA dehydrogenase is a separate protein (PMC3064157). That study identified a single-domain protein (CAI08632.1) with oxepin-CoA hydrolase activity, but it was ~1,000x more active as a crotonyl-CoA hydrolase; since we are not sure if its oxepin-CoA hydrolase activity is physiologically relevant, we did not include it. In Paraburkholderia bryophila 376MFSha3.1, which has a single-domain 3-oxo-5,6-didehydrosuberyl-CoA dehydrogenase, the putative enoyl-CoA hydrolase H281DRAFT_04594 (A0A2Z5MCI7) is very important for phenylacetate utilization, and we predict that it is the missing oxepin-CoA hydrolase. (H281DRAFT_04594 is related to enoyl-CoA hydratases that form (S)-3-hydroxylacyl-CoA, while the hydrolase domain of PaaZ is related to enoyl-CoA hydratases that form (R)-3-hydroxylacyl-CoA. In fact, PaaZ can dehydrate (R)-3-hydroxybutyryl-CoA (PMC3064157).)
Fetched 2 sequences
Running BLASTp
Found similarities, at above 30% identity and 75% coverage, for 0 of these sequences
Found 0 clusters of similar sequences. Another 2 sequences are not clustered. Download as table or as draft rules or view by organism
Singletons (0/2 heteromeric)
b1387 oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (EC 3.3.2.12; EC 1.2.1.91; EC 4.2.1.55) from Escherichia coli K-12 substr. MG1655
P77455 oxepin-CoA hydrolase/3-oxo-5,6-dehydrosuberyl-CoA semialdehyde dehydrogenase (EC 3.3.2.12; EC 1.2.1.91; EC 4.2.1.55) from Escherichia coli (strain K12)
PAAZ_ECOLI / P77455 Bifunctional protein PaaZ; EC 3.3.2.12; EC 1.2.1.91 from Escherichia coli (strain K12)
P77455 oxepin-CoA hydrolase (EC 3.3.2.12) from Escherichia coli
PFams: Aldedh, MaoC_dehydratas
681 amino acids: PaperBLAST, CDD
A0A2Z5MCI7 Enoyl-CoA hydratase; EC=4.2.1.17
262 amino acids: PaperBLAST, CDD