Clusters of Characterized Proteins

Clustering the characterized proteins for paaJ1 (3-oxo-5,6-dehydrosuberyl-CoA thiolase) in L-phenylalanine catabolism

Or see other characterized proteins similar to paaJ1

Or see all steps for L-phenylalanine catabolism

Or cluster curated proteins matching a keyword

Definition of paaJ1

• Curated proteins or TIGRFams with EC 2.3.1.223
• Ignore hits to items matching 2.3.1.174 when looking for 'other' hits
• Ignore hits to Q845J3 when looking for 'other' hits (3-oxo-5,6-didehydrosuberyl-CoA thiolase (EC 2.3.1.223))
• UniProt sequence B2SYZ2: RecName: Full=3-oxoadipyl-CoA thiolase {ECO:0000256|ARBA:ARBA00012233}; EC=2.3.1.174 {ECO:0000256|ARBA:ARBA00012233};
• UniProt sequence A0A2Z5MFE9: RecName: Full=3-oxoadipyl-CoA thiolase {ECO:0000256|ARBA:ARBA00012233}; EC=2.3.1.174 {ECO:0000256|ARBA:ARBA00012233};
• UniProt sequence D8ITH5: RecName: Full=3-oxoadipyl-CoA thiolase {ECO:0000256|ARBA:ARBA00012233}; EC=2.3.1.174 {ECO:0000256|ARBA:ARBA00012233};
• Curated sequence GFF2751: Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)
• Comment: PaaJ is a thiolase with two activities that are linked to two different EC numbers, so it is listed twice, as paaJ1 and paaJ2. The product of the first thiolase reaction should be 3,4-dehydroadipyl-CoA, not 2,3-dehydro-, so there is probably a second isomerization step, which might be catalyzed by paaG or by paaJ itself. In Burkholderia phytofirmans PsJN, this enzyme is BPHYT_RS17345 (B2SYZ2). In Paraburkholderia bryophila 376MFSha3.1, it is H281DRAFT_05723 (A0A2Z5MFE9). In Herbaspirillum seropedicae, it is HSERO_RS20660 (D8ITH5). In Marinobacter adhaerens, it is HP15_2695 (GFF2751). In BRENDA, Q845J3 is misannotated as paaJ; it is probably an accessory protein for assembly of the epoxidase (paaD, not included here).

Fetched 7 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 7 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 400-424 amino acids (not heteromeric)

P09110 3-ketoacyl-CoA thiolase, peroxisomal (EC 2.3.1.223; EC 2.3.1.16) from Homo sapiens
THIK_HUMAN / P09110 3-ketoacyl-CoA thiolase, peroxisomal; Acetyl-CoA acyltransferase; Beta-ketothiolase; Peroxisomal 3-oxoacyl-CoA thiolase; EC 2.3.1.16 from Homo sapiens (Human)
PFams: Thiolase_N, Thiolase_C
424 amino acids: PaperBLAST, CDD, Compare to cluster

b1397 β-ketoadipyl-CoA thiolase (EC 2.3.1.223; EC 2.3.1.174) from Escherichia coli K-12 substr. MG1655
P0C7L2 β-ketoadipyl-CoA thiolase (EC 2.3.1.223; EC 2.3.1.174) from Escherichia coli (strain K12)
PAAJ_ECOLI / P0C7L2 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase; EC 2.3.1.174; EC 2.3.1.223 from Escherichia coli (strain K12)
PFams: Thiolase_N, Thiolase_C
401 amino acids: PaperBLAST, CDD, Compare to cluster

A0A2Z5MFE9 3-oxoadipyl-CoA thiolase; EC=2.3.1.174
400 amino acids: PaperBLAST, CDD, Compare to cluster

B2SYZ2 3-oxoadipyl-CoA thiolase; EC=2.3.1.174
400 amino acids: PaperBLAST, CDD, Compare to cluster

D8ITH5 3-oxoadipyl-CoA thiolase; EC=2.3.1.174
401 amino acids: PaperBLAST, CDD, Compare to cluster

Q845J8 β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) from Pseudomonas sp. Y2
PFams: Thiolase_N, ketoacyl-synt, Thiolase_C
401 amino acids: PaperBLAST, CDD, Compare to cluster

HP15_2695 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) from Marinobacter adhaerens HP15
PFams: Thiolase_N, ketoacyl-synt, Thiolase_C
415 amino acids: PaperBLAST, CDD, Compare to cluster