Clusters of Characterized Proteins

GapMind for catabolism of small carbon sources

Clusters of Characterized Proteins

Clustering the characterized proteins for lctO (L-lactate oxidase or 2-monooxygenase) in L-threonine catabolism

Or see other characterized proteins similar to lctO

Or see all steps for L-threonine catabolism

Or cluster curated proteins matching a keyword

Definition of lctO

Curated proteins or TIGRFams with EC 1.13.12.4
Curated proteins or TIGRFams with EC 1.1.3.2
Ignore hits to items matching 1.1.3.15 when looking for 'other' hits
Comment: L-lactate oxidase (EC 1.13.12.4, formerly 1.1.3.2) oxidizes L-lactate to acetate and CO2 under aerobic conditions. Some of these enzymes produce pyruvate (and hydroxgen peroxide) instead, but are still given this EC number. Either way, the acetate can be used for growth. However this enzyme is mostly found in fermentative bacteria, so its role could be to detoxify the accumulated lactate. Since L-lactate is a (S)-2-hydroxy-acid, ignore any similarities to (S)-2-hydroxy-acid oxidases (1.1.3.15)

Fetched 7 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 7 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 365-407 amino acids (not heteromeric)

The first sequence in each cluster is the seed.

A9QH69 (S)-2-hydroxy-acid oxidase (EC 1.1.3.15); L-lactate oxidase (EC 1.1.3.2) from Streptococcus iniae
PFams: FMN_dh
390 amino acids: PaperBLAST, CDD, Compare to cluster

D4YFM2 lactate oxidase monomer (EC 1.1.3.2) from Aerococcus viridans ATCC 11563
D4YFM2 L-lactate oxidase (EC 1.1.3.2) from Aerococcus viridans
PFams: FMN_dh
374 amino acids: PaperBLAST, CDD, Compare to cluster

F8WQN2 L-lactate oxidase (EC 1.1.3.2) from Chlamydomonas reinhardtii
PFams: FMN_dh, IMPDH
384 amino acids: PaperBLAST, CDD, Compare to cluster

Q44467 L-lactate oxidase (EC 1.1.3.2) from Aerococcus viridans
PFams: FMN_dh
374 amino acids: PaperBLAST, CDD, Compare to cluster

Q8Z0C8 L-lactate oxidase (EC 1.1.3.2) from Nostoc sp. PCC 7120
PFams: FMN_dh
365 amino acids: PaperBLAST, CDD, Compare to cluster

LA2M_STRIN / O33655 Lactate 2-monooxygenase; Lactate oxidase; EC 1.13.12.4 from Streptococcus iniae (Streptococcus shiloi)
O33655 L-lactate oxidase (EC 1.1.3.2) from Streptococcus iniae
PFams: FMN_dh
403 amino acids: PaperBLAST, CDD, Compare to cluster

YKN3_SCHPO / Q9HDX2 Uncharacterized lactate 2-monooxygenase PB1A11.03; EC 1.13.12.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: FMN_dh
407 amino acids: PaperBLAST, CDD, Compare to cluster