Clusters of Characterized Proteins

Clustering the characterized proteins for trpA (indoleglycerol phosphate aldolase) in L-tryptophan biosynthesis

Or see other characterized proteins similar to trpA

Or see all steps for L-tryptophan biosynthesis

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Definition of trpA

• HMM TIGR00262
• Curated sequence Q9YGA9: tryptophan synthase subunit α (EC 4.1.2.8). tryptophan synthase (subunit 1/2) (EC 4.2.1.20)
• UniProt sequence A0A3N7DTH5: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
• UniProt sequence H0BV16: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
• UniProt sequence M4NLA4: RecName: Full=Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131};
• Ignore hits to items matching EC 4.2.1.20 when looking for 'other' hits
• Ignore hits to items matching EC 4.1.2.8 when looking for 'other' hits
• Comment: This reaction is 4.1.2.8 by itself or part of 4.2.1.20. The enzyme from Pyrolobus is not matched by the HMM, but is similar to the characterized trpA from Thermococcus (Q9YGA9, see PMID:11029579) APJEIK_06300 from Pedobacter sp. FW305-3-2-15-E-R2A2 (A0A3N7DTH5) can complement a trpA- strain of E. coli (Bradley Biggs). GAMENC_07330 from Acidovorax sp. FHTAMBA (H0BV16) can complement a trpA- strain of E. coli (Bradley Biggs); also it is very similar to Ac3H11_1535, which has auxotrophic phenotypes. LRK54_RS01680 from Rhodanobacter sp. FW104-10B01 (M4NLA4) can complement a trpA- strain of E. coli (Bradley Biggs); also it is similar to N515DRAFT_0116, which has auxotrophic pheonotypes.

Fetched 6 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 6 of these sequences

Found 2 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 251-269 amino acids (1/4 heteromeric)

A0A3N7DTH5 Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131}
255 amino acids: PaperBLAST, CDD, Compare to cluster

H0BV16 Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131}
269 amino acids: PaperBLAST, CDD, Compare to cluster

M4NLA4 Tryptophan synthase alpha chain {ECO:0000256|HAMAP-Rule:MF_00131}; EC=4.2.1.20 {ECO:0000256|HAMAP-Rule:MF_00131}
266 amino acids: PaperBLAST, CDD, Compare to cluster

Q9YGA9 tryptophan synthase subunit α (EC 4.1.2.8) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Q9YGA9 tryptophan synthase (subunit 1/2) (EC 4.2.1.20) from Thermococcus kodakarensis
PFams: Trp_syntA, Ribul_P_3_epim, His_biosynth
Heteromeric, 251 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 702-723 amino acids (not heteromeric)

CH_122413 bifunctional tryptophan synthase TRPB (from HMM only) from Emericella nidulans
PFams: Trp_syntA, PALP
723 amino acids: PaperBLAST, CDD, Compare to cluster

CH_123254 likely tryptophan synthetase alpha chain (from HMM only) from Candida albicans
PFams: Trp_syntA, PALP
702 amino acids: PaperBLAST, CDD, Compare to cluster