Clusters of Characterized Proteins

Clustering the characterized proteins for ech ((S)-3-hydroxybutanoyl-CoA hydro-lyase) in L-valine catabolism

Or see other characterized proteins similar to ech

Or see all steps for L-valine catabolism

Or cluster curated proteins matching a keyword

Definition of ech

• Curated proteins or TIGRFams with EC 4.2.1.150
• Ignore hits to Q97MS7 when looking for 'other' hits (short-chain-enoyl-CoA hydratase (EC 4.2.1.150))
• Curated sequence BPHYT_RS17335: trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17)
• Curated sequence GFF2389: Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17)
• Ignore hits to items matching 4.2.1.17 when looking for 'other' hits
• Comment: Psest_2437 (GFF2389) is the enoyl-CoA hydrotase for both isoleucine and valine degradation, which implies that (S)-3-hydroxybutanoyl-CoA is a substrate. Q97MS7 is misannotated in BRENDA. BPHYT_RS17335 was misannotated as paaF; it is very similar to the ech H16_A3307, which is a different explanation for its role in phenylacetate utilization. Short-chain enoyl-CoA hydratases are sometimes given EC 4.2.1.17 instead, so those are ignored.

Fetched 8 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 7 of these sequences

Found 2 clusters of similar sequences. Another 1 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 257-261 amino acids (not heteromeric)

P52046 crotonase monomer (EC 4.2.1.150) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
CRT_CLOAB / P52046 Short-chain-enoyl-CoA hydratase; 3-hydroxybutyryl-CoA dehydratase; Crotonase; EC 4.2.1.150 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
CH_091794 3-hydroxybutyryl-CoA dehydratase; EC 4.2.1.55 from Clostridium acetobutylicum
PFams: ECH_1, ECH_2
261 amino acids: PaperBLAST, CDD, Compare to cluster

CRCH_SYNWW / Q0AVM1 Crotonyl-CoA hydratase; EC 4.2.1.150 from Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
PFams: ECH_1, ECH_2
260 amino acids: PaperBLAST, CDD, Compare to cluster

A5N5C7 crotonase (EC 4.2.1.150) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
PFams: ECH_1, ECH_2
259 amino acids: PaperBLAST, CDD, Compare to cluster

BPHYT_RS17335 trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) from Burkholderia phytofirmans PsJN
PFams: ECH_1, ECH_2
258 amino acids: PaperBLAST, CDD, Compare to cluster

Psest_2437 Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) from Pseudomonas stutzeri RCH2
PFams: ECH_1, ECH_2
257 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 593-598 amino acids (not heteromeric)

CDYL_MOUSE / Q9WTK2 Chromodomain Y-like protein; CDY-like; Crotonyl-CoA hydratase; Putative histone acetyltransferase Cdyl; EC 4.2.1.-; EC 2.3.1.48 from Mus musculus (Mouse)
Q9WTK2 short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Mus musculus
PFams: Chromo, ECH_1, ECH_2
593 amino acids: PaperBLAST, CDD, Compare to cluster

CDYL_HUMAN / Q9Y232 Chromodomain Y-like protein; CDY-like; Crotonyl-CoA hydratase; EC 4.2.1.- from Homo sapiens (Human)
Q9Y232 short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Homo sapiens
PFams: Chromo, ECH_1, ECH_2
598 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/1 heteromeric)

A4YDS4 crotonyl-CoA hydratase/(S)-3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.35; EC 4.2.1.150) from Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)
A4YDS4 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) from Metallosphaera sedula
PFams: 3HCDH_N, NAD_binding_2, 3HCDH, 3HCDH, ECH_1, ECH_2
651 amino acids: PaperBLAST, CDD