Clusters of Characterized Proteins

Clustering the characterized proteins for prpF (methylaconitate isomerase) in L-valine catabolism

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Definition of prpF

• Curated proteins or TIGRFams with EC 5.3.3.7
• Curated sequence Q937N7: 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.-
• Ignore hits to A0A0A1H8I4 when looking for 'other' hits (Aconitate isomerase; AI; EC 5.3.3.7. aconitate DELTA-isomerase (EC 5.3.3.7))
• Comment: SwissProt annotates Q937N7 with this activity, but with a vague EC number. The periplasmic substrate-binding protein A0A0A1H8I4 has aconitate isomerase activity but has a high Km (PMID:26293748), and its periplasmic location would prevent it from participating in the methylcitrate cycle, so it is ignored.

Fetched 3 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 3 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 396-443 amino acids (not heteromeric)

PRPF_SHEON / Q8EJW4 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- from Shewanella oneidensis (strain MR-1)
Q8EJW4 aconitate DELTA-isomerase (EC 5.3.3.7) from Shewanella oneidensis
PFams: PrpF
397 amino acids: PaperBLAST, CDD, Compare to cluster

A0A0U2X0E4 aconitate-Δ-isomerase 1 (EC 5.3.3.7) from Ustilago maydis
ADI1_USTMD / A0A0U2X0E4 Aconitate-delta-isomerase 1; Itaconic acid/2-hydroxyparaconate biosynthesis cluster protein ADI1; EC 5.-.-.- from Ustilago maydis (Corn smut fungus)
PFams: PrpF
443 amino acids: PaperBLAST, CDD, Compare to cluster

PRPF_CUPNE / Q937N7 2-methyl-aconitate isomerase; Cis-trans isomerase; EC 5.3.3.- from Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha)
PFams: PrpF
396 amino acids: PaperBLAST, CDD, Compare to cluster