Other Characterized Proteins Similar to argA

GapMind for Amino acid biosynthesis

Other Characterized Proteins Similar to argA

Finding the characterized proteins for argA (N-acylglutamate synthase) in L-arginine biosynthesis

Or see clustering for step argA

Or see all steps for L-arginine biosynthesis

Or cluster curated proteins matching a keyword

Definition of argA

Curated proteins or TIGRFams with EC 2.3.1.1
Curated proteins matching N-succinylglutamate synthase
UniProt sequence Q8A1A5_BACTN: RecName: Full=GNAT family N-acetyltransferase {ECO:0008006|Google:ProtNLM};
UniProt sequence L0G3H4_ECHVK: SubName: Full=Acetyltransferase, N-acetylglutamate synthase {ECO:0000313|EMBL:AGA80057.1};
Curated sequence A0A0H2X8L7: acetylglutamate kinase (EC 2.7.2.8)
Curated sequence Q8P8J6: acetylglutamate kinase (EC 2.7.2.8)
Ignore hits to G6759-MONOMER when looking for 'other' hits (L-amino acid N-acyltransferase MnaT; L-methionine N-acyltransferase; L-methionine sulfoximine/L-methionine sulfone N-acetyltransferase; L-phenylglycine N-acetyltransferase; EC 2.3.1.-. L-amino acid N-acyltransferase (EC 2.3.1.1). L-amino acid N-acyltransferase (EC 2.3.1.1))
Ignore hits to items matching EC 2.3.1.35 when looking for 'other' hits
Ignore hits to CH_123299 when looking for 'other' hits (arginine biosynthetic enzyme activities)
Curated sequence CH_122594: glutamate N-acetyltransferase (Eurofung)
Ignore hits to Q9I3W7 when looking for 'other' hits (amino-acid N-acetyltransferase (EC 2.3.1.1))
Ignore hits to Q8P8J6 when looking for 'other' hits (acetylglutamate kinase (EC 2.7.2.8))
Predicted: UniProt sequence A0A127FCT3: RecName: Full=DUF1611 domain-containing protein {ECO:0008006|Google:ProtNLM};
Comment: Bacteroidetes have a divergent N-acylglutamate synthase, see BT3761 (Q8A1A5_BACTN) or Echvi_3845 (L0G3H4_ECHVK). Bacteroides use succinylated intermediates (PMID:16704984), so their proteins are probably N-succinylglutamate synthases. (These enzymes are also known as argA2, see PMC9026213.) Q8P8J6 is annotated as argB in BRENDA, but it is also argA (a fusion protein). N515DRAFT_3768 (A0A1I2DIM7) is similar to ArgAB fusion proteins and mutants are rescued by arginine. It is not clear if mnaT (link) would acetylate arginine, so it is ignored. Some EC 2.3.1.35 enzymes are probably dedicated for converting N-acetylornithine back to ornithine, while others are bifunctional for N-acetylglutamate formation as well (argJ); so similarity to this EC number is inconclusive. For some CharProtDB entries it is unclear if they have this activity or not, while others are labeled with this function but without the EC number. Q9I3W7 is annotated as this in BRENDA but we did not find experimental evidence of its activity on glutamate, so it is ignored. In Steroidobacter denitrificans, argA is missing but the arginine synthesis cluster includes a potential N-acetyltransferase ACG33_RS14135 (A0A127FCT3). Homologs of this protein are conserved in the cluster, and it is never found together with argA; it is distantly related to D-glutamate acetyltransferase dgcN (formerly DUF1611, see PMC10030869).

Fetched 33 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 10 other characterized sequences. (Found 40 hits including self hits.)

Removing ignored items reduces this to 2 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for argA.)

ARG56_YEAST / Q01217 Protein ARG5,6, mitochondrial; EC 1.2.1.38; EC 2.7.2.8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Q01217 acetylglutamate kinase (EC 2.7.2.8) from Saccharomyces cerevisiae
PFams: AA_kinase, NAT, Semialdhyde_dh, Semialdhyde_dhC
863 amino acids: PaperBLAST, CDD, BRENDA::Q01217
41% identical to

Q87EL2 amino-acid N-acetyltransferase (EC 2.3.1.1) from Xylella fastidiosa
PFams: AA_kinase, NAT
421 amino acids: PaperBLAST, CDD

ARG56_SCHPO / P31318 Protein arg11, mitochondrial; EC 1.2.1.38; EC 2.7.2.8 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
GI|1204210 N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase; EC 1.2.1.38; EC 2.7.2.8 from Schizosaccharomyces pombe
PFams: AA_kinase, NAT, Semialdhyde_dh
885 amino acids: PaperBLAST, CDD, CharProtDB::CH_125213
41% identical to

A0A0H2X8L7 acetylglutamate kinase (EC 2.7.2.8) from Xanthomonas campestris
PFams: AA_kinase, NAT
447 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for argA.)

Q8P8J6 acetylglutamate kinase (EC 2.7.2.8) from Xanthomonas campestris
PFams: AA_kinase, NAT
426 amino acids: PaperBLAST, CDD, BRENDA::Q8P8J6
100% identical to

A0A0H2X8L7 acetylglutamate kinase (EC 2.7.2.8) from Xanthomonas campestris
PFams: AA_kinase, NAT
447 amino acids: PaperBLAST, CDD

Q59280 glutamate N-acetyltransferase (EC 2.3.1.35) from Corynebacterium glutamicum
PFams: ArgJ
388 amino acids: PaperBLAST, CDD, BRENDA::Q59280
57% identical to

ARGJ_MYCTU / P9WPZ3 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WPZ3 glutamate N-acetyltransferase (EC 2.3.1.35) from Mycobacterium tuberculosis
PFams: ArgJ
Heteromeric, 404 amino acids: PaperBLAST, CDD

C1KGU6 glutamate N-acetyltransferase (EC 2.3.1.35) from Corynebacterium crenatum
PFams: ArgJ
388 amino acids: PaperBLAST, CDD, BRENDA::C1KGU6
57% identical to

P62058 glutamate N-acetyltransferase (EC 2.3.1.35) from Corynebacterium crenatum
PFams: ArgJ
388 amino acids: PaperBLAST, CDD, BRENDA::P62058
56% identical to

ARGJ_METJA / Q57645 Glutamate N-acetyltransferase; Ornithine acetyltransferase; OATase; Ornithine transacetylase; EC 2.3.1.35 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
PFams: ArgJ
Heteromeric, 402 amino acids: PaperBLAST, CDD, SwissProt::Q57645
43% identical to

ARGJ_THENN / Q9Z4S1 Arginine biosynthesis bifunctional protein ArgJ; EC 2.3.1.35; EC 2.3.1.1 from Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E)
PFams: ArgJ
Heteromeric, 397 amino acids: PaperBLAST, CDD

DVU0823 Glutamate N-acetyltransferase (EC 2.3.1.35) from Desulfovibrio vulgaris Hildenborough JW710
PFams: ArgJ
393 amino acids: PaperBLAST, CDD, reanno::DvH:206250
43% identical to

Q92MJ1 glutamate N-acetyltransferase (EC 2.3.1.35) from Sinorhizobium meliloti
PFams: ArgJ
413 amino acids: PaperBLAST, CDD, BRENDA::Q92MJ1
42% identical to

CH_000559 glutamate N-acetyltransferase/amino-acid acetyltransferase; EC 2.3.1.35 2.3.1.1 from Neisseria gonorrhoeae
PFams: ArgJ
406 amino acids: PaperBLAST, CDD

CH_123299 arginine biosynthetic enzyme activities from Candida albicans
PFams: AA_kinase, NAT, Semialdhyde_dh
857 amino acids: PaperBLAST, CDD, CharProtDB::CH_123299
40% identical to

N515DRAFT_3768 N-acetylglutamate synthase; N-acetylglutamate kinase (EC 2.3.1.1; EC 2.7.2.8) from Dyella japonica UNC79MFTsu3.2
PFams: AA_kinase, NAT
438 amino acids: PaperBLAST, CDD