Other Characterized Proteins Similar to argB

Finding the characterized proteins for argB (N-acylglutamate kinase) in L-arginine biosynthesis

Or see clustering for step argB

Or see all steps for L-arginine biosynthesis

Or cluster curated proteins matching a keyword

Definition of argB

• Curated proteins or TIGRFams with EC 2.7.2.8
• Curated proteins matching N-succinylglutamate kinase
• Ignore hits to O67848 when looking for 'other' hits (UDP-3-O-acyl-N-acetylglucosamine deacetylase (EC 3.5.1.108))
• Ignore hits to Q87EL2 when looking for 'other' hits (amino-acid N-acetyltransferase (EC 2.3.1.1))
• Curated sequence CH_123299: arginine biosynthetic enzyme activities
• Comment: ArgB includes Bacteroides proteins that act on N-succinylglutamate instead of the usual N-acetylglutamate (i.e. BT3395). See "Discovery of novel pathways of microbial arginine biosynthesis" (2010), PhD thesis of Juan Manuel Cabrera Luque, which shows that argB from B. fragilis is N-succinylglutamate kinase. O67848 in BRENDA seems likely to be argB but is misannotated as a deacetylase; also, Q87EL2 is likely to be argB as well as argA. CH_123299 has a broader annotation but probably has this activity.

Fetched 21 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 2 other characterized sequences. (Found 23 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

There are also 3 HMM-only high-coverage hits.

HMM-only sequences

(Since these sequences' annotations are outside the definition for argB, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

Q5SH27 [LysW]-L-2-aminoadipate 6-kinase monomer (EC 2.7.2.17) from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
PFams: AA_kinase
269 amino acids: PaperBLAST, CDD

LYSZ_THET2 / O50147 [LysW]-aminoadipate kinase; EC 2.7.2.17 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27)
PFams: AA_kinase
269 amino acids: PaperBLAST, CDD

Q4JAQ2 [LysW}-glutamate/[LysW]-aminoadipate kinase (EC 2.7.2.17; EC 2.7.2.19) from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
LYSZ_SULAC / Q4JAQ2 [LysW]-aminoadipate/[LysW]-glutamate kinase; EC 2.7.2.17; EC 2.7.2.19 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Q4JAQ2 [amino group carrier protein]-L-2-aminoadipate 6-kinase (EC 2.7.2.17) from Sulfolobus acidocaldarius
PFams: AA_kinase
261 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for argB.)

Q87EL2 amino-acid N-acetyltransferase (EC 2.3.1.1) from Xylella fastidiosa
PFams: AA_kinase, NAT
421 amino acids: PaperBLAST, CDD, BRENDA::Q87EL2
79% identical to

Q8P8J6 acetylglutamate kinase (EC 2.7.2.8) from Xanthomonas campestris
PFams: AA_kinase, NAT
426 amino acids: PaperBLAST, CDD

O67848 UDP-3-O-acyl-N-acetylglucosamine deacetylase (EC 3.5.1.108) from Aquifex aeolicus
PFams: AA_kinase
298 amino acids: PaperBLAST, CDD, BRENDA::O67848
54% identical to

ARGB_PSEAE / Q9HTN2 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Q9HTN2 acetylglutamate kinase (EC 2.7.2.8) from Pseudomonas aeruginosa
PFams: AA_kinase
301 amino acids: PaperBLAST, CDD