Other Characterized Proteins Similar to asnB

Finding the characterized proteins for asnB (asparagine synthase (glutamine-hydrolysing)) in L-asparagine biosynthesis

Or see clustering for step asnB

Or see all steps for L-asparagine biosynthesis

Or cluster curated proteins matching a keyword

Definition of asnB

• Curated proteins or TIGRFams with EC 6.3.5.4
• Ignore hits to A4I213 when looking for 'other' hits (asparagine synthase (glutamine-hydrolysing) (EC 6.3.5.4))
• Ignore hits to items matching EC 6.3.1.1 when looking for 'other' hits
• Ignore hits to CH_123066 when looking for 'other' hits (asparagine synthetase)
• Comment: In BRENDA, A4I213 is misannotated as asnB. Many asparagine synthases are bifunctional (obtaining nitrogen from either ammonia or glutamine) so similarity to EC 6.3.1.1 is ignored. CH_123066 has a vague annotation and it is not clear if it is actually characterized.

Fetched 38 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 1 other characterized sequences. (Found 39 hits including self hits.)

Removing ignored items reduces this to 0 close sequences.

There are also 6 HMM-only high-coverage hits.

HMM-only sequences

(Since these sequences' annotations are outside the definition for asnB, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

CHYD_PENRW / B6HLP8 Amidase chyE; Chrysogine biosynthesis cluster protein D; EC 3.-.-.- from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum)
PFams: GATase_6, GATase_7, Asn_synthase
672 amino acids: PaperBLAST, CDD

Q92W82 N-acetylglutaminylglutamine amide synthase from Rhizobium meliloti (strain 1021)
PFams: GATase_6, GATase_7, Asn_synthase
591 amino acids: PaperBLAST, CDD

E2JA33 N-3-fumaroyl-(S)-2,3-diaminopropanoate aminotransferase from Enterobacter agglomerans
PFams: GATase_6, GATase_7, NAD_synthase, Asn_synthase
607 amino acids: PaperBLAST, CDD

Q9CDJ2 peptidoglycan asparagine synthase from Lactococcus lactis subsp. lactis (strain IL1403)
PFams: GATase_6, GATase_7, Asn_synthase
625 amino acids: PaperBLAST, CDD

L8EVF2 malonamoyl-CoA synthetase from Streptomyces rimosus subsp. rimosus (strain ATCC 10970 / DSM 40260 / JCM 4667 / NRRL 2234)
PFams: GATase_6, GATase_7, Asn_synthase
612 amino acids: PaperBLAST, CDD

CHRY2_GIBZE / I1S3K8 Amidase chry2; Chrysogine biosynthesis cluster protein 2; EC 3.-.-.- from Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
PFams: GATase_6, GATase_7, Asn_synthase
671 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for asnB.)

CH_123066 asparagine synthetase from Candida albicans
PFams: GATase_6, GATase_7, Asn_synthase, Asn_synthase
573 amino acids: PaperBLAST, CDD, CharProtDB::CH_123066
74% identical to

ASNS2_YEAST / P49090 Asparagine synthetase [glutamine-hydrolyzing] 2; Glutamine-dependent asparagine synthetase 2; EC 6.3.5.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: GATase_6, GATase_7, DUF3700, Asn_synthase, Asn_synthase
572 amino acids: PaperBLAST, CDD