Other Characterized Proteins Similar to aspS2

GapMind for Amino acid biosynthesis

Other Characterized Proteins Similar to aspS2

Finding the characterized proteins for aspS2 (aspartyl-tRNA(Asp/Asn) synthetase) in L-asparagine biosynthesis

Or see clustering for step aspS2

Or see all steps for L-asparagine biosynthesis

Or cluster curated proteins matching a keyword

Definition of aspS2

Curated proteins or TIGRFams with EC 6.1.1.23
UniProt sequence A0A0H3C7V8_CAUVN: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_DINSH: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
UniProt sequence A0A2I7KAZ8: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence B8DMM5_DESVM: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_DESVH: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_SYNE7: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
Ignore hits to P9WFW3 when looking for 'other' hits (aspartate-tRNA ligase (EC 6.1.1.12))
Ignore hits to ASPS-MONOMER when looking for 'other' hits (Aspartate--tRNA ligase; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12. aspartate—tRNA ligase (EC 6.1.1.12). aspartate—tRNA ligase (EC 6.1.1.12; EC 6.1.1.23))
Ignore hits to MONOMER-124420 when looking for 'other' hits (aspartate--tRNA ligase/ergosteryl-3β-O-L-aspartate synthase (EC 6.1.1.23; EC 2.3.2.37))
Comment: AspS2 forms both Asp-tRNA(Asp) and Asp-tRNA(Asn). It is difficult to distinguish the "non-discriminatory" synthase (aspS2) from the discriminatory synthase (aspRS) by similarity. Also, the presence of the tRNA-dependent amidotransferase gatABC is not sufficient to conclude that aspS2 is present because gatABC are also involved in tRNA-dependent synthesis of glutamine. However, if aspargine synthase and asparginyl-tRNA synthetase (asnRS) are absent, then we can conclude that the aspartyl-tRNA synthetase is non-discriminatory. This is the basis for annotating CCNA_01969 (A0A0H3C7V8_CAUVN), Dshi_2633 (SYDND_DINSH), and PGA1_c24530 (A0A2I7KAZ8). In Desulfovibrio vulgaris (2 strains) and in Synechococcus, the situation is more complicated -- there is an asnRS, but it is not essential, or even improtant for fitness in most conditions. This also indicates the presence of the tRNA-dependent pathway. (It is also doubtful whether any of those genomes encode asnB or asnA.) This is the basis for annotating DvMF_2038 (B8DMM5_DESVM), DVU3367 (SYDND_DESVH), and Synpcc7942_1313 (SYDND_SYNE7). Although the asp-tRNA synthetase from Mycobacterium tuberculosis (P9WFW3) is annotated as aspartate-specific in BRENDA, this organism seems to lack asparaginyl-tRNA synthetase, so this is ignored. MetaCyc annotates E.coli aspS (link) as both discriminating and non-discriminating, which is incorrect, so this is ignored. And MetaCyc gives erdS (link) this EC number, but it is not reported to act on asparagine.

Fetched 33 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 6 other characterized sequences. (Found 33 hits including self hits.)

Removing ignored items reduces this to 3 close sequences.

There are also 9 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for aspS2.)

SYD_THETH / P36419 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermus thermophilus
SYD_THET8 / Q5SKD2 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase 1; AspRS1; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
P36419 aspartate-tRNA ligase (EC 6.1.1.12) from Thermus thermophilus
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
580 amino acids: PaperBLAST, CDD, BRENDA::P36419
59% identical to

Q9RUN7 aspartyl-tRNA synthetase subunit (EC 6.1.1.23) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
SYD_DEIRA / Q9RUN7 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase 1; AspRS1; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
577 amino acids: PaperBLAST, CDD

SYDM_ARATH / F4JJT9 Aspartate--tRNA ligase, chloroplastic/mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Arabidopsis thaliana (Mouse-ear cress)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
664 amino acids: PaperBLAST, CDD, SwissProt::F4JJT9
58% identical to

SYDND_SYNE7 Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044}
599 amino acids: PaperBLAST, CDD

SYDM_HUMAN / Q6PI48 Aspartate--tRNA ligase, mitochondrial; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Homo sapiens (Human)
Q6PI48 aspartate-tRNA ligase (EC 6.1.1.12) from Homo sapiens
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
645 amino acids: PaperBLAST, CDD, BRENDA::Q6PI48
43% identical to

O32038 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Bacillus subtilis
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
592 amino acids: PaperBLAST, CDD

HMM-only sequences

(Since these sequences' annotations are outside the definition for aspS2, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

SYDC2_ARATH / Q9M084 Aspartate--tRNA ligase 2, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Protein IMPAIRED IN BABA-INDUCED DISEASE IMMUNITY 1; EC 6.1.1.12 from Arabidopsis thaliana (Mouse-ear cress)
PFams: tRNA_anti-codon, tRNA-synt_2
558 amino acids: PaperBLAST, CDD

SYDC_YEAST / P04802 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: tRNA_anti-codon, tRNA-synt_2
557 amino acids: PaperBLAST, CDD

SYDC_MOUSE / Q922B2 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Mus musculus (Mouse)
Q922B2 aspartate-tRNA ligase (EC 6.1.1.12) from Mus musculus
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD

SYDC_RAT / P15178 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Rattus norvegicus (Rat)
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD

Q585M3 aspartate-tRNA ligase (EC 6.1.1.12) from Trypanosoma brucei
PFams: tRNA_anti-codon, tRNA-synt_2
560 amino acids: PaperBLAST, CDD

SYD_THEKO / Q52428 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Q52428 aspartate-tRNA ligase (EC 6.1.1.12) from Thermococcus kodakarensis
PFams: tRNA_anti-codon, tRNA-synt_2
438 amino acids: PaperBLAST, CDD

SYDC_SCHPO / O74407 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: tRNA_anti-codon, tRNA-synt_2
580 amino acids: PaperBLAST, CDD

SYDC_HUMAN / P14868 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; EC 6.1.1.12 from Homo sapiens (Human)
P14868 aspartate-tRNA ligase (EC 6.1.1.12) from Homo sapiens
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD

Q8I2B1 aspartate-tRNA ligase (EC 6.1.1.12) from Plasmodium falciparum
PFams: tRNA_anti-codon, tRNA-synt_2
626 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for aspS2.)

b1866 aspartate—tRNA ligase (EC 6.1.1.12) from Escherichia coli K-12 substr. MG1655
P21889 aspartate—tRNA ligase (EC 6.1.1.12; EC 6.1.1.23) from Escherichia coli (strain K12)
SYD_ECOLI / P21889 Aspartate--tRNA ligase; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 from Escherichia coli (strain K12)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
590 amino acids: PaperBLAST, CDD, SwissProt::P21889
63% identical to

SYDND_PSEAE / Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
591 amino acids: PaperBLAST, CDD

P9WFW3 aspartate-tRNA ligase (EC 6.1.1.12) from Mycobacterium tuberculosis
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
594 amino acids: PaperBLAST, CDD, BRENDA::P9WFW3
49% identical to

Q4WKG3 aspartate--tRNA ligase/ergosteryl-3β-O-L-aspartate synthase (EC 6.1.1.23; EC 2.3.2.37) from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293)
PFams: tRNA-synt_2, LPG_synthase_C
947 amino acids: PaperBLAST, CDD, metacyc::MONOMER-124420
45% identical to