Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for aspS2 (aspartyl-tRNA(Asp/Asn) synthetase) in L-asparagine biosynthesis

Or see other characterized proteins similar to aspS2

Or see all steps for L-asparagine biosynthesis

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Definition of aspS2

Curated proteins or TIGRFams with EC 6.1.1.23
UniProt sequence A0A0H3C7V8_CAUVN: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_DINSH: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
UniProt sequence A0A2I7KAZ8: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence B8DMM5_DESVM: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_DESVH: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
UniProt sequence SYDND_SYNE7: RecName: Full=Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044};
Ignore hits to P9WFW3 when looking for 'other' hits (aspartate-tRNA ligase (EC 6.1.1.12))
Ignore hits to ASPS-MONOMER when looking for 'other' hits (Aspartate--tRNA ligase; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12. aspartate—tRNA ligase (EC 6.1.1.12). aspartate—tRNA ligase (EC 6.1.1.12; EC 6.1.1.23))
Ignore hits to MONOMER-124420 when looking for 'other' hits (aspartate--tRNA ligase/ergosteryl-3β-O-L-aspartate synthase (EC 6.1.1.23; EC 2.3.2.37))
Comment: AspS2 forms both Asp-tRNA(Asp) and Asp-tRNA(Asn). It is difficult to distinguish the "non-discriminatory" synthase (aspS2) from the discriminatory synthase (aspRS) by similarity. Also, the presence of the tRNA-dependent amidotransferase gatABC is not sufficient to conclude that aspS2 is present because gatABC are also involved in tRNA-dependent synthesis of glutamine. However, if aspargine synthase and asparginyl-tRNA synthetase (asnRS) are absent, then we can conclude that the aspartyl-tRNA synthetase is non-discriminatory. This is the basis for annotating CCNA_01969 (A0A0H3C7V8_CAUVN), Dshi_2633 (SYDND_DINSH), and PGA1_c24530 (A0A2I7KAZ8). In Desulfovibrio vulgaris (2 strains) and in Synechococcus, the situation is more complicated -- there is an asnRS, but it is not essential, or even improtant for fitness in most conditions. This also indicates the presence of the tRNA-dependent pathway. (It is also doubtful whether any of those genomes encode asnB or asnA.) This is the basis for annotating DvMF_2038 (B8DMM5_DESVM), DVU3367 (SYDND_DESVH), and Synpcc7942_1313 (SYDND_SYNE7). Although the asp-tRNA synthetase from Mycobacterium tuberculosis (P9WFW3) is annotated as aspartate-specific in BRENDA, this organism seems to lack asparaginyl-tRNA synthetase, so this is ignored. MetaCyc annotates E.coli aspS (link) as both discriminating and non-discriminating, which is incorrect, so this is ignored. And MetaCyc gives erdS (link) this EC number, but it is not reported to act on asparagine.

Fetched 33 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 33 of these sequences

Found 2 clusters of similar sequences. Download as table or as draft rules or view by cluster

Alkalihalobacillus halodurans

Q9KDG1 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Alkalihalobacillus halodurans
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
595 amino acids: PaperBLAST, CDD (Cluster 1)

Arabidopsis thaliana

SYDC2_ARATH / Q9M084 Aspartate--tRNA ligase 2, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Protein IMPAIRED IN BABA-INDUCED DISEASE IMMUNITY 1; EC 6.1.1.12 (from HMM only) from Arabidopsis thaliana (Mouse-ear cress)
PFams: tRNA_anti-codon, tRNA-synt_2
558 amino acids: PaperBLAST, CDD (Cluster 2)

Bacillus subtilis

O32038 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Bacillus subtilis
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
592 amino acids: PaperBLAST, CDD (Cluster 1)

Bdellovibrio bacteriovorus

Q6MI59 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Bdellovibrio bacteriovorus
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
642 amino acids: PaperBLAST, CDD (Cluster 1)

Caulobacter crescentus

CCNA_01969 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Caulobacter crescentus NA1000
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
612 amino acids: PaperBLAST, CDD (Cluster 1)

Chlamydia trachomatis

SYDND_CHLTR / O84546 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Chlamydia trachomatis (strain D/UW-3/Cx)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
582 amino acids: PaperBLAST, CDD (Cluster 1)

Deinococcus radiodurans

Q9RUN7 aspartyl-tRNA synthetase subunit (EC 6.1.1.23) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
SYD_DEIRA / Q9RUN7 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase 1; AspRS1; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
577 amino acids: PaperBLAST, CDD (Cluster 1)

Q9RVH4 aspartyl-tRNA synthetase, non-discriminating (EC 6.1.1.23) from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
SYDND_DEIRA / Q9RVH4 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase 2; AspRS2; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / CCUG 27074 / LMG 4051 / NBRC 15346 / NCIMB 9279 / VKM B-1422 / R1)
PFams: tRNA_anti-codon, tRNA-synt_2
435 amino acids: PaperBLAST, CDD (Cluster 2)

Desulfovibrio vulgaris

DvMF_2038 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Desulfovibrio vulgaris Miyazaki F
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
614 amino acids: PaperBLAST, CDD (Cluster 1)

Dinoroseobacter shibae

Dshi_2633 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Dinoroseobacter shibae DFL-12
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
590 amino acids: PaperBLAST, CDD (Cluster 1)

Entamoeba histolytica

C4LZN0 aspartate-tRNA ligase (EC 6.1.1.12); aspartate-tRNAAsn ligase (EC 6.1.1.23) from Entamoeba histolytica
PFams: tRNA_anti-codon, tRNA-synt_2
544 amino acids: PaperBLAST, CDD (Cluster 2)

Halobacterium salinarum

SYDND_HALSA / O07683 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
O07683 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Halobacterium salinarum
PFams: tRNA_anti-codon, tRNA-synt_2
436 amino acids: PaperBLAST, CDD (Cluster 2)

Helicobacter pylori

SYDND_HELPY / P56459 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
P56459 aspartate-tRNA ligase (EC 6.1.1.12) from Helicobacter pylori
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
577 amino acids: PaperBLAST, CDD (Cluster 1)

Homo sapiens

SYDC_HUMAN / P14868 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; EC 6.1.1.12 (from HMM only) from Homo sapiens (Human)
P14868 aspartate-tRNA ligase (EC 6.1.1.12) from Homo sapiens
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD (Cluster 2)

Methanosarcina mazei

SYDND_METMA / Q8Q0R2 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
PFams: tRNA_anti-codon, tRNA-synt_2
444 amino acids: PaperBLAST, CDD (Cluster 2)

Methanothermobacter thermautotrophicus

SYDND_METTH / O26328 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum)
O26328 aspartate-tRNAAsn ligase (EC 6.1.1.23) from Methanothermobacter thermautotrophicus
PFams: tRNA_anti-codon, tRNA-synt_2
437 amino acids: PaperBLAST, CDD (Cluster 2)

Mus musculus

SYDC_MOUSE / Q922B2 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 (from HMM only) from Mus musculus (Mouse)
Q922B2 aspartate-tRNA ligase (EC 6.1.1.12) from Mus musculus
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD (Cluster 2)

Organism unknown

A0A0H3C7V8_CAUVN Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044}
612 amino acids: PaperBLAST, CDD (Cluster 1)

A0A2I7KAZ8 Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044}
592 amino acids: PaperBLAST, CDD (Cluster 1)

B8DMM5_DESVM Aspartate--tRNA(Asp/Asn) ligase {ECO:0000256|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000256|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000256|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000256|HAMAP-Rule:MF_00044}
614 amino acids: PaperBLAST, CDD (Cluster 1)

SYDND_DESVH Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044}
610 amino acids: PaperBLAST, CDD (Cluster 1)

SYDND_DINSH Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044}
590 amino acids: PaperBLAST, CDD (Cluster 1)

SYDND_SYNE7 Aspartate--tRNA(Asp/Asn) ligase {ECO:0000255|HAMAP-Rule:MF_00044}; EC=6.1.1.23 {ECO:0000255|HAMAP-Rule:MF_00044}; Aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=AspRS {ECO:0000255|HAMAP-Rule:MF_00044}; AltName: Full=Non-discriminating aspartyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00044}; Short=ND-AspRS {ECO:0000255|HAMAP-Rule:MF_00044}
599 amino acids: PaperBLAST, CDD (Cluster 1)

Phaeobacter inhibens

PGA1_c24530 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Phaeobacter inhibens BS107
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
592 amino acids: PaperBLAST, CDD (Cluster 1)

Plasmodium falciparum

Q8I2B1 aspartate-tRNA ligase (EC 6.1.1.12) (from HMM only) from Plasmodium falciparum
PFams: tRNA_anti-codon, tRNA-synt_2
626 amino acids: PaperBLAST, CDD (Cluster 2)

Pseudomonas aeruginosa

SYDND_PSEAE / Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
591 amino acids: PaperBLAST, CDD (Cluster 1)

Rattus norvegicus

SYDC_RAT / P15178 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 (from HMM only) from Rattus norvegicus (Rat)
PFams: tRNA_anti-codon, tRNA-synt_2
501 amino acids: PaperBLAST, CDD (Cluster 2)

Saccharomyces cerevisiae

SYDC_YEAST / P04802 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 (from HMM only) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: tRNA_anti-codon, tRNA-synt_2
557 amino acids: PaperBLAST, CDD (Cluster 2)

Schizosaccharomyces pombe

SYDC_SCHPO / O74407 Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; EC 6.1.1.12 (from HMM only) from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
PFams: tRNA_anti-codon, tRNA-synt_2
580 amino acids: PaperBLAST, CDD (Cluster 2)

Synechococcus elongatus

Synpcc7942_1313 aspartyl-tRNA(Asp/Asn) synthetase (EC 6.1.1.23) from Synechococcus elongatus PCC 7942
PFams: tRNA_anti-codon, tRNA-synt_2, GAD
599 amino acids: PaperBLAST, CDD (Cluster 1)

Thermococcus kodakarensis

SYD_THEKO / Q52428 Aspartate--tRNA(Asp) ligase; Aspartyl-tRNA synthetase; AspRS; Discriminating aspartyl-tRNA synthetase; D-AspRS; EC 6.1.1.12 (from HMM only) from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
Q52428 aspartate-tRNA ligase (EC 6.1.1.12) from Thermococcus kodakarensis
PFams: tRNA_anti-codon, tRNA-synt_2
438 amino acids: PaperBLAST, CDD (Cluster 2)

Thermus thermophilus

SYDND_THET8 / Q5SIC2 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase 2; AspRS2; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
Q5SIC2 aspartate-tRNA ligase (EC 6.1.1.12) from Thermus thermophilus
PFams: tRNA_anti-codon, tRNA-synt_2
422 amino acids: PaperBLAST, CDD (Cluster 2)

Trypanosoma brucei

Q585M3 aspartate-tRNA ligase (EC 6.1.1.12) (from HMM only) from Trypanosoma brucei
PFams: tRNA_anti-codon, tRNA-synt_2
560 amino acids: PaperBLAST, CDD (Cluster 2)