Other Characterized Proteins Similar to aroE

Finding the characterized proteins for aroE (shikimate dehydrogenase) in chorismate biosynthesis

Or see clustering for step aroE

Or see all steps for chorismate biosynthesis

Or cluster curated proteins matching a keyword

Definition of aroE

• Curated proteins or TIGRFams with EC 1.1.1.25
• Curated proteins or TIGRFams with EC 1.1.1.282
• UniProt sequence Q8A006_BACTN: SubName: Full=Shikimate 5-dehydrogenase {ECO:0000313|EMBL:AAO79320.1};
• UniProt sequence A0A2M8WD96: RecName: Full=Shikimate dehydrogenase (NADP(+)) {ECO:0000256|HAMAP-Rule:MF_00222}; Short=SDH {ECO:0000256|HAMAP-Rule:MF_00222}; EC=1.1.1.25 {ECO:0000256|HAMAP-Rule:MF_00222};
• Ignore hits to CH_122204 when looking for 'other' hits (quinate dehydrogenase; EC 1.1.1.24. quinate dehydrogenase (EC 1.1.1.282))
• Comment: EC 1.1.1.282 is with NAD(P)H instead of NADPH. BT4215 from Bacteroides thetaiotaomicron (Q8A006_BACTN) is diverged, is the only good candidate, and is essential in various Bacteroidetes. Ga0059261_2194 / BDW16_RS10815 (A0A2M8WD96) has auxotrophic phenotypes in RB-TnSeq data and can complement an aroE- strain of E. coli (Bradley Biggs). CH_122204 in CharProtDB is ignored because it is probably quinate dehydrogenase, not shikimate dehydrogenase.

Fetched 48 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 2 other characterized sequences. (Found 48 hits including self hits.)

There are also 1 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for aroE.)

A0A5H2X0F8 quinate/shikimate dehydrogenase (NAD+) (EC 1.1.1.24) from Eucalyptus camaldulensis
PFams: DHquinase_I, Shikimate_dh_N, SDH_C
520 amino acids: PaperBLAST, CDD, BRENDA::A0A5H2X0F8
69% identical to

D7TUX1 shikimate dehydrogenase (NADP+) (EC 1.1.1.25) from Vitis vinifera
PFams: DHquinase_I, Shikimate_dh_N, SDH_C
519 amino acids: PaperBLAST, CDD

A0A5H2WXM2 quinate/shikimate dehydrogenase (NAD+) (EC 1.1.1.24) from Eucalyptus camaldulensis
PFams: DHquinase_I, Shikimate_dh_N, SDH_C
520 amino acids: PaperBLAST, CDD, BRENDA::A0A5H2WXM2
69% identical to

D7TUX1 shikimate dehydrogenase (NADP+) (EC 1.1.1.25) from Vitis vinifera
PFams: DHquinase_I, Shikimate_dh_N, SDH_C
519 amino acids: PaperBLAST, CDD

HMM-only sequences

(Since these sequences' annotations are outside the definition for aroE, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

A0A095ULP3 5-ketofructose reductase (NADP) monomer (EC 1.1.1.124) from Tatumella morbirosei
PFams: Shikimate_dh_N, Shikimate_DH, SDH_C
284 amino acids: PaperBLAST, CDD