Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for aroL (shikimate kinase) in chorismate biosynthesis

Or see other characterized proteins similar to aroL

Or see all steps for chorismate biosynthesis

Or cluster curated proteins matching a keyword

Definition of aroL

Curated proteins or TIGRFams with EC 2.7.1.71
UniProt sequence AROK_BACSU: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_BACTN: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence L0FT15_ECHVK: RecName: Full=Shikimate kinase {ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|HAMAP-Rule:MF_00109};
UniProt sequence AROK_DESVH: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_CAUVN: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_RHIME: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence I7EWF3_PHAIB: RecName: Full=Shikimate kinase {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109};
Comment: In E. coli, AroL and AroK are isozymes. In Bacillus subtilis, this gene was known as AroI, and it was cloned by complementation (see A. Nakane et al, J. Fermentation and Bioengineering 1994, 77:312-314.) That sequence is identical to AROK_BACSU. Manually add BT3393 (AROK_BACTN) from B. thetaiotaomicron because it is diverged, is the only good candidate, and is essential in various Bacteroidetes. Similarly for Echvi_0140 (L0FT15_ECHVK) from Echinicola vietnamensis. And DVU0892 (AROK_DESVH) from D. vulgaris Hildenborough is confirmed by cofitness CCNA_03103 (AROK_CAUVN) is confirmed by cofitness and similar proteins such as SMc00695 (AROK_RHIME) and PGA1_c14090 (I7EWF3_PHAIB) are essential.

Fetched 22 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 21 of these sequences

Found 3 clusters of similar sequences. Another 3 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 162-200 amino acids (not heteromeric)

A0A0M3KL09 shikimate kinase (EC 2.7.1.71)
PFams: AAA_33, SKI
179 amino acids: PaperBLAST, CDD, Compare to cluster

P56073 Shikimate kinase; SK; EC 2.7.1.71
P56073 shikimate kinase (EC 2.7.1.71)
PFams: SKI
162 amino acids: PaperBLAST, CDD, Compare to cluster

P9WPY3 Shikimate kinase; SK; EC 2.7.1.71
P9WPY3 shikimate kinase (EC 2.7.1.71)
PFams: AAA_33, AAA_18, SKI
176 amino acids: PaperBLAST, CDD, Compare to cluster

AROK-MONOMER shikimate kinase 1 (EC 2.7.1.71)
P0A6D7 Shikimate kinase 1; SK 1; Shikimate kinase I; SKI; EC 2.7.1.71
PFams: AAA_18, AAA_33, Cytidylate_kin, SKI
173 amino acids: PaperBLAST, CDD, Compare to cluster

AROL-MONOMER shikimate kinase 2 (EC 2.7.1.71)
AROL-MONOMER shikimate kinase II (EC 2.7.1.71)
P0A6E1 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71
PFams: AAA_18, AAA, SKI
174 amino acids: PaperBLAST, CDD, Compare to cluster

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71
PFams: AAA_18, AAA_33, SKI
173 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_BACSU Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
186 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_BACTN Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
175 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_CAUVN Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
200 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_DESVH Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
175 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_RHIME Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
192 amino acids: PaperBLAST, CDD, Compare to cluster

I7EWF3_PHAIB Shikimate kinase {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}
196 amino acids: PaperBLAST, CDD, Compare to cluster

L0FT15_ECHVK Shikimate kinase {ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|HAMAP-Rule:MF_00109}
176 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 287-308 amino acids (not heteromeric)

Q7X7H9 Shikimate kinase 3, chloroplastic; OsSK3; EC 2.7.1.71
Q7X7H9 shikimate kinase (EC 2.7.1.71)
PFams: AAA_18, SKI
287 amino acids: PaperBLAST, CDD, Compare to cluster

Q5NTH4 Shikimate kinase 1, chloroplastic; OsSK1; EC 2.7.1.71
Q5NTH4 shikimate kinase (EC 2.7.1.71)
PFams: AAA_18, SKI
308 amino acids: PaperBLAST, CDD, Compare to cluster

Q00497 Shikimate kinase, chloroplastic; EC 2.7.1.71
PFams: AAA_18, SKI
300 amino acids: PaperBLAST, CDD, Compare to cluster

AT2G21940-MONOMER shikimate kinase (EC 2.7.1.71)
Q9SJ05 Shikimate kinase 1, chloroplastic; AtSK1; EC 2.7.1.71
PFams: AAA_18, SKI
303 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 1583-1588 amino acids (not heteromeric)

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25
P07547 shikimate dehydrogenase (EC 1.1.1.25)
CH_122161 pentafunctional AROM polypeptide; EC 1.1.1.25; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 4.2.3.4
PFams: Fe-ADH_2, DHQ_synthase, EPSP_synthase, SKI, DHquinase_I, Shikimate_dh_N, SDH_C
1583 amino acids: PaperBLAST, CDD, Compare to cluster

YDR127W-MONOMER pentafunctional AROM polypeptide (EC 4.2.3.4; EC 1.1.1.25; EC 2.7.1.71; EC 2.5.1.19; EC 4.2.1.10)
P08566 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25
PFams: DHQ_synthase, EPSP_synthase, SKI, DHquinase_I, Shikimate_dh_N, Shikimate_DH, SDH_C
1588 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/3 heteromeric)

Q5NTH3 Shikimate kinase 2, chloroplastic; OsSK2; EC 2.7.1.71
Q5NTH3 shikimate kinase (EC 2.7.1.71)
PFams: CoaE, AAA_18, SKI
307 amino acids: PaperBLAST, CDD
(similar to BRENDA::Q5NTH4 from Cluster 2, but similarity to seed sequence is below thresholds)

AT4G39540-MONOMER shikimate kinase (EC 2.7.1.71)
Q8GY88 Shikimate kinase 2, chloroplastic; AtSK2; EC 2.7.1.71
Q8GY88 shikimate kinase (EC 2.7.1.71)
PFams: AAA_18, CoaE, SKI
300 amino acids: PaperBLAST, CDD
(similar to SwissProt::Q00497 from Cluster 2, but similarity to seed sequence is below thresholds)

MONOMER-18801 shikimate kinase (EC 2.7.1.71)
Q58835 Shikimate kinase; SK; EC 2.7.1.71
PFams: GHMP_kinases_N, GHMP_kinases_C
282 amino acids: PaperBLAST, CDD