Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for aroL (shikimate kinase) in chorismate biosynthesis

Or see other characterized proteins similar to aroL

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Definition of aroL

Curated proteins or TIGRFams with EC 2.7.1.71
UniProt sequence AROK_BACSU: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_BACTN: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence L0FT15_ECHVK: RecName: Full=Shikimate kinase {ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|HAMAP-Rule:MF_00109};
UniProt sequence AROK_DESVH: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_CAUVN: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence AROK_RHIME: RecName: Full=Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109};
UniProt sequence A0A135IJ25: RecName: Full=Shikimate kinase {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109};
Comment: In E. coli, AroL and AroK are isozymes. In Bacillus subtilis, this gene was known as AroI, and it was cloned by complementation (see A. Nakane et al, J. Fermentation and Bioengineering 1994, 77:312-314.) That sequence is identical to AROK_BACSU. Manually add BT3393 (AROK_BACTN) from B. thetaiotaomicron because it is diverged, is the only good candidate, and is essential in various Bacteroidetes. Similarly for Echvi_0140 (L0FT15_ECHVK) from Echinicola vietnamensis. And DVU0892 (AROK_DESVH) from D. vulgaris Hildenborough is confirmed by cofitness CCNA_03103 (AROK_CAUVN) is confirmed by cofitness and similar proteins such as SMc00695 (AROK_RHIME) and PGA1_c14090 (A0A135IJ25) are essential.

Fetched 24 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 23 of these sequences

Found 3 clusters of similar sequences. Another 3 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 162-200 amino acids (not heteromeric)

A0A0M3KL09 shikimate kinase (EC 2.7.1.71) from Acinetobacter baumannii
PFams: AAA_33, SKI
179 amino acids: PaperBLAST, CDD, Compare to cluster

b3390 shikimate kinase 1 (EC 2.7.1.71) from Escherichia coli K-12 substr. MG1655
P0A6D7 shikimate kinase 1 (EC 2.7.1.71) from Escherichia coli (strain K12)
AROK_ECOLI / P0A6D7 Shikimate kinase 1; SK 1; Shikimate kinase I; SKI; EC 2.7.1.71 from Escherichia coli (strain K12)
P0A6D7 shikimate kinase (EC 2.7.1.71) from Escherichia coli
PFams: AAA_18, AAA_33, SKI
173 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_MYCTU / P9WPY3 Shikimate kinase; SK; EC 2.7.1.71 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
P9WPY3 shikimate kinase (EC 2.7.1.71) from Mycobacterium tuberculosis
PFams: AAA_33, AAA_18, SKI
176 amino acids: PaperBLAST, CDD, Compare to cluster

Q6GGG1 shikimate kinase (EC 2.7.1.71) from Staphylococcus aureus
PFams: AAA_18, SKI
174 amino acids: PaperBLAST, CDD, Compare to cluster

b0388 shikimate kinase 2 (EC 2.7.1.71) from Escherichia coli K-12 substr. MG1655
P0A6E1 shikimate kinase 2 (EC 2.7.1.71) from Escherichia coli (strain K12)
AROL_ECOLI / P0A6E1 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Escherichia coli (strain K12)
PFams: AAA_18, AAA, SKI
174 amino acids: PaperBLAST, CDD, Compare to cluster

AROL_DICCH / P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi)
PFams: AAA_18, AAA_33, SKI
173 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_HELPY / P56073 Shikimate kinase; SK; EC 2.7.1.71 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
PFams: AAA_18, SKI
162 amino acids: PaperBLAST, CDD, Compare to cluster

A0A135IJ25 Shikimate kinase {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|ARBA:ARBA00012154, ECO:0000256|HAMAP-Rule:MF_00109}
196 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_BACSU Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
186 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_BACTN Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
175 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_CAUVN Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
200 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_DESVH Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
175 amino acids: PaperBLAST, CDD, Compare to cluster

AROK_RHIME Shikimate kinase {ECO:0000255|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000255|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000255|HAMAP-Rule:MF_00109}
192 amino acids: PaperBLAST, CDD, Compare to cluster

L0FT15_ECHVK Shikimate kinase {ECO:0000256|HAMAP-Rule:MF_00109}; Short=SK {ECO:0000256|HAMAP-Rule:MF_00109}; EC=2.7.1.71 {ECO:0000256|HAMAP-Rule:MF_00109}
176 amino acids: PaperBLAST, CDD, Compare to cluster

CCNA_03103 shikimate kinase (EC 2.7.1.71) from Caulobacter crescentus NA1000
PFams: SKI
200 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 2 287-308 amino acids (not heteromeric)

SK3_ORYSJ / Q7X7H9 Shikimate kinase 3, chloroplastic; OsSK3; EC 2.7.1.71 from Oryza sativa subsp. japonica (Rice)
Q7X7H9 shikimate kinase (EC 2.7.1.71) from Oryza sativa
PFams: AAA_18, SKI
287 amino acids: PaperBLAST, CDD, Compare to cluster

SK1_ORYSJ / Q5NTH4 Shikimate kinase 1, chloroplastic; OsSK1; EC 2.7.1.71 from Oryza sativa subsp. japonica (Rice)
Q5NTH4 shikimate kinase (EC 2.7.1.71) from Oryza sativa
PFams: AAA_18, SKI
308 amino acids: PaperBLAST, CDD, Compare to cluster

SK_SOLLC / Q00497 Shikimate kinase, chloroplastic; EC 2.7.1.71 from Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
PFams: AAA_18, SKI
300 amino acids: PaperBLAST, CDD, Compare to cluster

Q9SJ05 shikimate kinase (EC 2.7.1.71) from Arabidopsis thaliana
SK1_ARATH / Q9SJ05 Shikimate kinase 1, chloroplastic; AtSK1; EC 2.7.1.71 from Arabidopsis thaliana (Mouse-ear cress)
PFams: AAA_18, SKI
303 amino acids: PaperBLAST, CDD, Compare to cluster

Cluster 3 1583-1588 amino acids (not heteromeric)

ARO1_EMENI / P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
P07547 shikimate dehydrogenase (NADP+) (EC 1.1.1.25) from Aspergillus nidulans
CH_122161 pentafunctional AROM polypeptide; EC 1.1.1.25; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 4.2.3.4 from Emericella nidulans
PFams: DHQ_synthase, Fe-ADH_2, EPSP_synthase, SKI, DHquinase_I, Shikimate_dh_N, SDH_C
1583 amino acids: PaperBLAST, CDD, Compare to cluster

P08566 pentafunctional AROM polypeptide (EC 4.2.3.4; EC 1.1.1.25; EC 2.7.1.71; EC 2.5.1.19; EC 4.2.1.10) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
ARO1_YEAST / P08566 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: DHQ_synthase, EPSP_synthase, SKI, DHquinase_I, Shikimate_dh_N, Shikimate_DH, SDH_C
1588 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/3 heteromeric)

SK2_ORYSJ / Q5NTH3 Shikimate kinase 2, chloroplastic; OsSK2; EC 2.7.1.71 from Oryza sativa subsp. japonica (Rice)
Q5NTH3 shikimate kinase (EC 2.7.1.71) from Oryza sativa
PFams: AAA_18, SKI
307 amino acids: PaperBLAST, CDD
(similar to BRENDA::Q5NTH4 from Cluster 2, but similarity to seed sequence is below thresholds)

Q8GY88 shikimate kinase (EC 2.7.1.71) from Arabidopsis thaliana
SK2_ARATH / Q8GY88 Shikimate kinase 2, chloroplastic; AtSK2; EC 2.7.1.71 from Arabidopsis thaliana (Mouse-ear cress)
Q8GY88 shikimate kinase (EC 2.7.1.71) from Arabidopsis thaliana
PFams: AAA_18, SKI
300 amino acids: PaperBLAST, CDD
(similar to SwissProt::Q00497 from Cluster 2, but similarity to seed sequence is below thresholds)

Q58835 shikimate kinase (EC 2.7.1.71) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
AROK_METJA / Q58835 Shikimate kinase; SK; EC 2.7.1.71 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
PFams: GHMP_kinases_N, GHMP_kinases_C
282 amino acids: PaperBLAST, CDD