Clusters of Characterized Proteins

Clustering the characterized proteins for cimA ((R)-citramalate synthase) in L-isoleucine biosynthesis

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Definition of cimA

• Curated proteins or TIGRFams with EC 2.3.1.182
• UniProt sequence Q8F3Q1_LEPIN: RecName: Full=(R)-citramalate synthase CimA {ECO:0000305}; EC=2.3.1.182 {ECO:0000269|PubMed:15292141, ECO:0000269|PubMed:18498255, ECO:0000269|PubMed:19351325}; AltName: Full=LiCMS {ECO:0000303|PubMed:18498255};
• UniProt sequence D4GSQ2: SubName: Full=2-isopropylmalate synthase / (R)-citramalatesynthase {ECO:0000313|EMBL:ADE04954.1}; EC=2.3.1.182 {ECO:0000313|EMBL:ADE04954.1}; EC=2.3.3.13 {ECO:0000313|EMBL:ADE04954.1};
• Comment: MetaCyc L-isoleucine biosynthesis II describes the formation of 2-oxobutanoate via citramalate. The other steps are the same (although it gives a different EC number for ilvC because of different cofactor preference) The citramalate synthase from Leptopsira interrogans (LA_2350, NP_712531, or Q8F3Q1_LEPIN) has been characterized biochemically but is not in the curated databases, see PMID:18498255 The putative citramalate synthase HVO_0644 (D4GSQ2) from Haloferax volcanii is required for isoleucine biosynthesis, see PMC4300041

Fetched 5 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 5 of these sequences

Found 1 clusters of similar sequences. Another 1 sequences are not clustered. Download as table or as draft rules or view by organism

Cluster 1 491-520 amino acids (not heteromeric)

Q58787 (R)-citramalate synthase CimA; EC 2.3.1.182
PFams: HMGL-like, LeuA_dimer
491 amino acids: PaperBLAST, CDD, Compare to cluster

B0K6M2 (R)-citramalate synthase (EC 2.3.1.182)
PFams: HMGL-like, LeuA_dimer
520 amino acids: PaperBLAST, CDD, Compare to cluster

D4GSQ2 2-isopropylmalate synthase / (R)-citramalatesynthase; EC=2.3.1.182; EC=2.3.3.13
512 amino acids: PaperBLAST, CDD, Compare to cluster

Q8F3Q1_LEPIN (R)-citramalate synthase CimA; EC=2.3.1.182 {ECO:0000269|PubMed:15292141, ECO:0000269|PubMed:18498255, ECO:0000269|PubMed:19351325}; LiCMS {ECO:0000303|PubMed:18498255}
516 amino acids: PaperBLAST, CDD, Compare to cluster

Singletons (0/1 heteromeric)

Q74C76 (R)-citramalate synthase; Citramalate synthase; EC 2.3.1.182
Q74C76 (R)-citramalate synthase (EC 2.3.1.182)
PFams: HMGL-like, LeuA_dimer
528 amino acids: PaperBLAST, CDD
(similar to BRENDA::B0K6M2 from Cluster 1, but similarity to seed sequence is below thresholds)