Other Characterized Proteins Similar to ilvA

Finding the characterized proteins for ilvA (threonine deaminase) in L-isoleucine biosynthesis

Or see clustering for step ilvA

Or see all steps for L-isoleucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of ilvA

• Curated proteins or TIGRFams with EC 4.3.1.19
• Ignore hits to items matching threonine deaminase when looking for 'other' hits
• Curated sequence B1N2N4: L-serine ammonia-lyase (EC 4.3.1.17)
• Ignore hits to P09367 when looking for 'other' hits (L-serine ammonia-lyase (EC 4.3.1.17))
• Comment: (Ignore some CharProtDB annotations with threonine deaminase but no EC). B1N2N4 is included because it is active on both serine and threonine (PMID:19931317). P09367 is ignored because it may be active on threonine as well as serine.

Fetched 23 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 4 other characterized sequences. (Found 27 hits including self hits.)

Removing ignored items reduces this to 2 close sequences.

There are also 1 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for ilvA.)

A0A0X1KHE6 L-serine ammonia-lyase (EC 4.3.1.17) from Rhizomucor miehei
PFams: PALP
366 amino acids: PaperBLAST, CDD, BRENDA::A0A0X1KHE6
42% identical to

P20132 L-serine dehydratase (EC 4.3.1.19; EC 4.3.1.17) from Homo sapiens
SDHL_HUMAN / P20132 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Homo sapiens (Human)
P20132 L-serine ammonia-lyase (EC 4.3.1.17) from Homo sapiens
PFams: PALP
328 amino acids: PaperBLAST, CDD

A0QY48 L-serine ammonia-lyase (EC 4.3.1.17) from Mycolicibacterium smegmatis
PFams: PALP
316 amino acids: PaperBLAST, CDD, BRENDA::A0QY48
41% identical to

TSAL_THEMA / Q9WYJ1 L-threonine ammonia-lyase; L-serine ammonia-lyase; Threonine dehydratase; EC 4.3.1.19; EC 4.3.1.17 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
PFams: PALP, ACT
401 amino acids: PaperBLAST, CDD

HMM-only sequences

(Since these sequences' annotations are outside the definition for ilvA, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

Q2V0H1 serine racemase (EC 5.1.1.18) from Pyrobaculum islandicum
PFams: PALP, ACT
402 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for ilvA.)

CH_124219 threonine deaminase [Arxula adeninivorans] from Magnaporthe grisea 70-15
PFams: PALP, Thr_dehydrat_C, Thr_dehydrat_C
618 amino acids: PaperBLAST, CDD, CharProtDB::CH_124219
74% identical to

V6QU65 threonine ammonia-lyase (EC 4.3.1.19) from Fusarium graminearum
PFams: PALP, Thr_dehydrat_C, Thr_dehydrat_C
581 amino acids: PaperBLAST, CDD

P09367 L-serine ammonia-lyase (EC 4.3.1.17) from Rattus norvegicus
PFams: PALP, PALP
363 amino acids: PaperBLAST, CDD, BRENDA::P09367
84% identical to

P20132 L-serine dehydratase (EC 4.3.1.19; EC 4.3.1.17) from Homo sapiens
SDHL_HUMAN / P20132 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Homo sapiens (Human)
P20132 L-serine ammonia-lyase (EC 4.3.1.17) from Homo sapiens
PFams: PALP
328 amino acids: PaperBLAST, CDD