Other Characterized Proteins Similar to ilvD

Finding the characterized proteins for ilvD (dihydroxy-acid dehydratase) in L-isoleucine biosynthesis

Or see clustering for step ilvD

Or see all steps for L-isoleucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of ilvD

• Curated proteins or TIGRFams with EC 4.2.1.9
• Ignore hits to MONOMER-15882 when looking for 'other' hits (dihydroxy-acid dehydratase)
• Comment: IlvD is involved in the biosynthesis of isoleucine, with (R)-2,3-dihydroxy-3-methylpentanoate as the substrate, and in the biosynthesis of valine and leucine, with (R)-2,3-dihydroxy-3-methylbutanoate as the substrate. The ignored enzyme is involved in salinosporamide A biosynthesis but does a very similar reaction and is >50% identical to N515DRAFT_0569, which is confirmed by fitness data to be biosynthetic

Fetched 12 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 2 other characterized sequences. (Found 14 hits including self hits.)

Removing ignored items reduces this to 1 close sequences.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for ilvD.)

SM_b20890 L-arabonate dehydratase (EC 4.2.1.25) from Sinorhizobium meliloti 1021
PFams: ILVD_EDD
579 amino acids: PaperBLAST, CDD, reanno::Smeli:SM_b20890
40% identical to

O27498 dihydroxy-acid dehydratase subunit (EC 4.2.1.9) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
PFams: ILVD_EDD
549 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for ilvD.)

B0L7G4 dihydroxy-acid dehydratase from Salinispora tropica
PFams: ILVD_EDD
592 amino acids: PaperBLAST, CDD, metacyc::MONOMER-15882
50% identical to

ILVD_MYCTU / P9WKJ5 Dihydroxy-acid dehydratase; DAD; EC 4.2.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
PFams: ILVD_EDD
575 amino acids: PaperBLAST, CDD