Clusters of Characterized Proteins

GapMind for Amino acid biosynthesis

Clusters of Characterized Proteins

Clustering the characterized proteins for ofoa (2-oxobutanoate:ferredoxin oxidoreductase, alpha subunit) in L-isoleucine biosynthesis

Or see other characterized proteins similar to ofoa

Or see all steps for L-isoleucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of ofoa

UniProt sequence OFOA1_SULTO: RecName: Full=2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha {ECO:0000303|PubMed:27619895}; Short=OFOR1 {ECO:0000303|PubMed:27619895}; EC=1.2.7.11 {ECO:0000269|PubMed:27619895, ECO:0000305|PubMed:19027887};
UniProt sequence OFOA_SULSP: RecName: Full=2-oxoacid:ferredoxin oxidoreductase subunit alpha {ECO:0000303|PubMed:8902625}; Short=OFOR {ECO:0000303|PubMed:11683888}; EC=1.2.7.11 {ECO:0000269|PubMed:11683888, ECO:0000269|PubMed:12009405, ECO:0000269|PubMed:8902625};
UniProt sequence OFOA_SACSO: RecName: Full=2-oxoacid:ferredoxin oxidoreductase subunit alpha {ECO:0000303|PubMed:16466637}; Short=OFOR {ECO:0000305}; EC=1.2.7.11 {ECO:0000269|PubMed:16466637};
UniProt sequence OFOA2_SULTO: RecName: Full=2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha {ECO:0000303|PubMed:27619895}; Short=OFOR2 {ECO:0000303|PubMed:27619895}; EC=1.2.7.11 {ECO:0000269|PubMed:27619895};
UniProt sequence OFOA1_AERPE: RecName: Full=2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha {ECO:0000303|PubMed:15848165}; Short=OFOR1 {ECO:0000303|PubMed:15848165}; EC=1.2.7.11 {ECO:0000269|PubMed:15848165};
UniProt sequence OFOA2_AERPE: RecName: Full=2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha {ECO:0000303|PubMed:15848165}; Short=OFOR2 {ECO:0000303|PubMed:15848165}; EC=1.2.7.11 {ECO:0000269|PubMed:15848165};
Comment: The key reaction is alpha-ketobutyrate synthase or 2-oxobutanoate:ferredoxin oxidoreductase (in reverse) These are heterodimeric enzymes and the only one mentioned by MetaCyc is an enzyme from Sulfolobus tokodaii 7 that includes ST2300 (alpha subunit, OFOA_SULSP). The beta subunit is OFOB_SULSP (but metacyc seems not to know this). Some related enzymes also are believed to do this

Fetched 6 sequences

Running BLASTp

Found similarities, at above 30% identity and 75% coverage, for 6 of these sequences

Found 1 clusters of similar sequences. Download as table or as draft rules or view by organism

Cluster 1 627-642 amino acids (not heteromeric)

The first sequence in each cluster is the seed.

OFOA1_AERPE 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha {ECO:0000303|PubMed:15848165}; Short=OFOR1 {ECO:0000303|PubMed:15848165}; EC=1.2.7.11 {ECO:0000269|PubMed:15848165}
639 amino acids: PaperBLAST, CDD, Compare to cluster

OFOA1_SULTO 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha {ECO:0000303|PubMed:27619895}; Short=OFOR1 {ECO:0000303|PubMed:27619895}; EC=1.2.7.11 {ECO:0000269|PubMed:27619895, ECO:0000305|PubMed:19027887}
627 amino acids: PaperBLAST, CDD, Compare to cluster

OFOA2_AERPE 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha {ECO:0000303|PubMed:15848165}; Short=OFOR2 {ECO:0000303|PubMed:15848165}; EC=1.2.7.11 {ECO:0000269|PubMed:15848165}
642 amino acids: PaperBLAST, CDD, Compare to cluster

OFOA2_SULTO 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha {ECO:0000303|PubMed:27619895}; Short=OFOR2 {ECO:0000303|PubMed:27619895}; EC=1.2.7.11 {ECO:0000269|PubMed:27619895}
628 amino acids: PaperBLAST, CDD, Compare to cluster

OFOA_SACSO 2-oxoacid:ferredoxin oxidoreductase subunit alpha {ECO:0000303|PubMed:16466637}; Short=OFOR; EC=1.2.7.11 {ECO:0000269|PubMed:16466637}
632 amino acids: PaperBLAST, CDD, Compare to cluster

OFOA_SULSP 2-oxoacid:ferredoxin oxidoreductase subunit alpha {ECO:0000303|PubMed:8902625}; Short=OFOR {ECO:0000303|PubMed:11683888}; EC=1.2.7.11 {ECO:0000269|PubMed:11683888, ECO:0000269|PubMed:12009405, ECO:0000269|PubMed:8902625}
632 amino acids: PaperBLAST, CDD, Compare to cluster