Other Characterized Proteins Similar to leuA

Finding the characterized proteins for leuA (2-isopropylmalate synthase) in L-leucine biosynthesis

Or see clustering for step leuA

Or see all steps for L-leucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of leuA

• Curated proteins or TIGRFams with EC 2.3.3.13
• UniProt sequence L0G5E1_ECHVK: SubName: Full=Isopropylmalate/homocitrate/citramalate synthase {ECO:0000313|EMBL:AGA80045.1};
• UniProt sequence A0A1X9Z750_9SPHI: SubName: Full=2-isopropylmalate synthase {ECO:0000313|EMBL:ARS41048.1};
• Ignore hits to items matching isopropylmalate synthase when looking for 'other' hits
• Comment: Ignore CharProtDB entries with no EC number. Add Echvi_3833 (L0G5E1_ECHVK) and CA265_RS15855 (A0A1X9Z750_9SPHI), which are supported by fitness data

Fetched 23 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 6 other characterized sequences. (Found 26 hits including self hits.)

Removing ignored items reduces this to 5 close sequences.

There are also 1 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for leuA.)

Q8VX04 Methylthioalkylmalate synthase 2, chloroplastic; EC 2.3.3.17
Q8VX04 methylthioalkylmalate synthase (EC 2.3.3.17)
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD, BRENDA::Q8VX04
95% identical to

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17)
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD

A0A0B5GPY2 methylthioalkylmalate synthase (EC 2.3.3.17)
PFams: HMGL-like
387 amino acids: PaperBLAST, CDD, BRENDA::A0A0B5GPY2
80% identical to

Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17)
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD

Q58787 (R)-citramalate synthase CimA; EC 2.3.1.182
PFams: HMGL-like, LeuA_dimer
491 amino acids: PaperBLAST, CDD, SwissProt::Q58787
51% identical to

Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13
Q58595 2-isopropylmalate synthase (EC 2.3.3.13)
PFams: HMGL-like, LeuA_dimer
518 amino acids: PaperBLAST, CDD

Q57926 Probable homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.-
PFams: HMGL-like
406 amino acids: PaperBLAST, CDD, SwissProt::Q57926
41% identical to

L0G5E1_ECHVK Isopropylmalate/homocitrate/citramalate synthase
390 amino acids: PaperBLAST, CDD

P58637 homocitrate synthase (EC 2.3.3.14)
PFams: HMGL-like
376 amino acids: PaperBLAST, CDD, BRENDA::P58637
42% identical to

Q58595 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13
Q58595 2-isopropylmalate synthase (EC 2.3.3.13)
PFams: HMGL-like, LeuA_dimer
518 amino acids: PaperBLAST, CDD

HMM-only sequences

(Since these sequences' annotations are outside the definition for leuA, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

D0VY45 homocitrate synthase (EC 2.3.3.14)
PFams: HMGL-like, LeuA_dimer
540 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for leuA.)

CH_124190 2-isopropylmalate synthase 2
PFams: HMGL-like, LeuA_dimer
616 amino acids: PaperBLAST, CDD, CharProtDB::CH_124190
57% identical to

P06208 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13
PFams: HMGL-like, LeuA_dimer
619 amino acids: PaperBLAST, CDD