Other Characterized Proteins Similar to leuA

GapMind for Amino acid biosynthesis

Other Characterized Proteins Similar to leuA

Finding the characterized proteins for leuA (2-isopropylmalate synthase) in L-leucine biosynthesis

Or see clustering for step leuA

Or see all steps for L-leucine biosynthesis

Or cluster curated proteins matching a keyword

Definition of leuA

Curated proteins or TIGRFams with EC 2.3.3.13
UniProt sequence L0G5E1_ECHVK: SubName: Full=Isopropylmalate/homocitrate/citramalate synthase {ECO:0000313|EMBL:AGA80045.1};
UniProt sequence A0A1X9Z750_9SPHI: SubName: Full=2-isopropylmalate synthase {ECO:0000313|EMBL:ARS41048.1};
Ignore hits to items matching isopropylmalate synthase when looking for 'other' hits
Ignore hits to P85362 when looking for 'other' hits (2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13)
Ignore hits to S3D9F8 when looking for 'other' hits (Isopropyl malate synthase gloH; L-homotyrosine biosynthesis sub-cluster protein gloH; Pneumocandin biosynthesis cluster protein H; EC 2.3.3.13)
Ignore hits to K0E4E5 when looking for 'other' hits (Isopropyl malate synthase htyA; L-homotyrosine biosynthetic cluster protein A; EC 2.3.3.13. 2-(4-hydroxybenzyl)-malate synthase)
Comment: Ignore CharProtDB entries with no EC number. Add Echvi_3833 (L0G5E1_ECHVK) and CA265_RS15855 (A0A1X9Z750_9SPHI), which are supported by fitness data. P85362 is ignored because it is just 9 amino acids. S3D9F8 and K0E4E5 are involved in secondary metabolite synthesis and it is not clear if they actually have this activity, so they are ignored.

Fetched 31 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 10 other characterized sequences. (Found 39 hits including self hits.)

Removing ignored items reduces this to 7 close sequences.

There are also 1 HMM-only high-coverage hits.

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for leuA.)

MAM2_ARATH / Q8VX04 Methylthioalkylmalate synthase 2, chloroplastic; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress)
Q8VX04 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD, SwissProt::Q8VX04
95% identical to

Q9FG67 methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
MAM1_ARATH / Q9FG67 Methylthioalkylmalate synthase 1, chloroplastic; 2-isopropylmalate synthase 3; EC 2.3.3.17 from Arabidopsis thaliana (Mouse-ear cress)
Q9FG67 2-isopropylmalate synthase (EC 2.3.3.13); methylthioalkylmalate synthase (EC 2.3.3.17) from Arabidopsis thaliana
PFams: HMGL-like
506 amino acids: PaperBLAST, CDD

A0A0B5GPY2 methylthioalkylmalate synthase (EC 2.3.3.17) from Brassica oleracea
PFams: HMGL-like
387 amino acids: PaperBLAST, CDD, BRENDA::A0A0B5GPY2
80% identical to

Q58787 (R)-citratemalate synthase subunit (EC 2.3.3.21) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
CIMA_METJA / Q58787 (R)-citramalate synthase CimA; EC 2.3.3.21 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
Q58787 (R)-citramalate synthase (EC 2.3.3.21) from Methanocaldococcus jannaschii
PFams: HMGL-like, LeuA_dimer
491 amino acids: PaperBLAST, CDD, BRENDA::Q58787
53% identical to

LEU1_SULAC / Q4JA78 2-isopropylmalate synthase; IPMS; Alpha-isopropylmalate synthase; Alpha-IPM synthase; EC 2.3.3.13 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
PFams: HMGL-like
386 amino acids: PaperBLAST, CDD

AKSA_METJA / Q57926 Homocitrate synthase AksA; (R)-homo(2)citrate synthase; (R)-homo(3)citrate synthase; EC 2.3.3.14; EC 2.3.3.- from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)
PFams: HMGL-like
406 amino acids: PaperBLAST, CDD, SwissProt::Q57926
46% identical to

P58637 homocitrate synthase (EC 2.3.3.14) from Nostoc sp. PCC 7120 = FACHB-418
PFams: HMGL-like
376 amino acids: PaperBLAST, CDD, BRENDA::P58637
46% identical to

HOSA_SULTO / Q971S5 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii)
PFams: HMGL-like, AsnC_trans_reg
460 amino acids: PaperBLAST, CDD, SwissProt::Q971S5
43% identical to

HOSA_SULAC / Q4J989 Homocitrate synthase; HCS; EC 2.3.3.14 from Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Q4J989 homocitrate synthase (EC 2.3.3.14) from Sulfolobus acidocaldarius
PFams: HMGL-like, AsnC_trans_reg
468 amino acids: PaperBLAST, CDD, BRENDA::Q4J989
42% identical to

HMM-only sequences

(Since these sequences' annotations are outside the definition for leuA, HMM hits that are over 40% similar to these sequences will be scored as moderate confidence.)

D0VY45 homocitrate synthase (EC 2.3.3.14) from Lotus japonicus
PFams: HMGL-like, LeuA_dimer
540 amino acids: PaperBLAST, CDD

Close but ignored sequences

(Sequences that are similar to these will still be high-confidence candidates for leuA.)

CH_124190 2-isopropylmalate synthase 2 from Magnaporthe grisea 70-15
PFams: HMGL-like, LeuA_dimer
616 amino acids: PaperBLAST, CDD, CharProtDB::CH_124190
57% identical to

LEU1_YEAST / P06208 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
PFams: HMGL-like, LeuA_dimer
619 amino acids: PaperBLAST, CDD

K0E4E5 2-(4-hydroxybenzyl)-malate synthase from Aspergillus rugulosus
HTYA_ASPRU / K0E4E5 Isopropyl malate synthase htyA; L-homotyrosine biosynthetic cluster protein A; EC 2.3.3.13 from Aspergillus rugulosus (Emericella rugulosa)
PFams: HMGL-like
584 amino acids: PaperBLAST, CDD, SwissProt::K0E4E5
51% identical to

AMT7_ALTAL / C9K7B8 Isopropyl malate synthase AMT7; AM-toxin biosynthesis protein 7; EC 2.3.3.13 from Alternaria alternata (Alternaria rot fungus) (Torula alternata)
PFams: HMGL-like
582 amino acids: PaperBLAST, CDD

GLOH_GLAL2 / S3D9F8 Isopropyl malate synthase gloH; L-homotyrosine biosynthesis sub-cluster protein gloH; Pneumocandin biosynthesis cluster protein H; EC 2.3.3.13 from Glarea lozoyensis (strain ATCC 20868 / MF5171)
PFams: HMGL-like
617 amino acids: PaperBLAST, CDD, SwissProt::S3D9F8
49% identical to