Other Characterized Proteins Similar to dapE

Finding the characterized proteins for dapE (succinyl-diaminopimelate desuccinylase) in L-lysine biosynthesis

Or see clustering for step dapE

Or see all steps for L-lysine biosynthesis

Or cluster curated proteins matching a keyword

Definition of dapE

• Curated proteins or TIGRFams with EC 3.5.1.18
• Predicted: UniProt sequence L0FXC2: SubName: Full=Amidohydrolase {ECO:0000313|EMBL:AGA77693.1};
• Comment: DapE is missing in several genera of Bacteroidota, and a search for conserved essential genes identified the putative aminohydrolase Echvi_1427 (L0FXC2)as a candidate. This protein is 43% identical to the dipeptidase DradM20D (PMID:26456402) and 42% identical to an carboxypeptidase/aminoacylase from Pyrococcus horikoshii (PMC92634); it also has more distant homology to N-acetyl-L,L-diaminopimelate deactylase; these are all very similar reactions. Echvi_1427 has conserved active site residues and docking N-succinyl-L,L-diaminopimelate to its predicted structure with AutoDock Vina gave predicted binding (-6.5 kcal/mol) with the amide bond near the catalytic arginine (R264).

Fetched 10 sequences

Running ublast to find other characterized proteins with 40% identity and 70% coverage

Found hits to 3 other characterized sequences. (Found 12 hits including self hits.)

Close sequences

(Sequences that are similar to these will not be high-confidence candidates for dapE.)

O58754 N-acyl-aliphatic-L-amino acid amidohydrolase (EC 3.5.1.14) from Pyrococcus horikoshii
PFams: Peptidase_M20, M20_dimer
387 amino acids: PaperBLAST, CDD, BRENDA::O58754
41% identical to

L0FXC2 Amidohydrolase
397 amino acids: PaperBLAST, CDD

O58453 N-acyl-aliphatic-L-amino acid amidohydrolase (EC 3.5.1.14) from Pyrococcus horikoshii
PFams: Peptidase_M20, M20_dimer
388 amino acids: PaperBLAST, CDD, BRENDA::O58453
41% identical to

L0FXC2 Amidohydrolase
397 amino acids: PaperBLAST, CDD

ILR1_ARATH / P54968 IAA-amino acid hydrolase ILR1; EC 3.5.1.- from Arabidopsis thaliana (Mouse-ear cress)
PFams: Peptidase_M20, M20_dimer
442 amino acids: PaperBLAST, CDD, SwissProt::P54968
40% identical to

L0FXC2 Amidohydrolase
397 amino acids: PaperBLAST, CDD